Base de dados : MEDLINE
Pesquisa : D09.254.330 [Categoria DeCS]
Referências encontradas : 888 [refinar]
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[PMID]:28853563
[Au] Autor:Wright EP; Lamparska K; Smith SS; Waller ZAE
[Ad] Endereço:School of Pharmacy, University of East Anglia , Norwich Research Park, Norwich NR4 7TJ, U.K.
[Ti] Título:Substitution of Cytosine with Guanylurea Decreases the Stability of i-Motif DNA.
[So] Source:Biochemistry;56(36):4879-4883, 2017 Sep 12.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Both 5-aza-2'-deoxycytidine (decitabine) and its primary breakdown product, 2'-deoxyriboguanylurea (GuaUre-dR), have been shown to act as mutagens and epimutagens that cause replication stress and alter both DNA methylation and gene expression patterns. As cytosine analogues, both are expected to be preferentially incorporated into regions of GC skew where runs of cytosine residues are sequestered on one strand and guanine residues on the other. Given that such regions have been identified as sites with the potential for effects on gene expression and replication stress linked to formation of alternative DNA secondary structures, it is of interest to determine the influence that these base analogues might have on the stability of structures of this kind. Here we report that incorporation of GuaUre-dR into an i-motif-forming sequence decreases both the thermal and pH stability of an i-motif despite the apparent ability of GuaUre-dR to base pair with cytosine.
[Mh] Termos MeSH primário: Citosina/química
DNA/química
Desoxirribose/análogos & derivados
Guanidinas/química
[Mh] Termos MeSH secundário: Sequência de Bases
Dicroísmo Circular
Desoxirribose/química
Seres Humanos
Conformação de Ácido Nucleico
Mutação Puntual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2'-deoxyriboguanylurea); 0 (Guanidines); 533-67-5 (Deoxyribose); 8J337D1HZY (Cytosine); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00628


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[PMID]:27991936
[Au] Autor:Sadowska-Bartosz I; Galiniak S; Bartosz G
[Ad] Endereço:Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.
[Ti] Título:Modification of the deoxyribose test to detect strong iron binding.
[So] Source:Acta Biochim Pol;64(1):195-198, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe concentration, at a constant concentration of a chelating agent.
[Mh] Termos MeSH primário: Desoxirribose/química
Peróxido de Hidrogênio/química
Radical Hidroxila/química
Ferro/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Quelantes/química
Técnicas de Laboratório Clínico/métodos
Relação Dose-Resposta a Droga
Quelantes de Ferro/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Fenton's reagent); 0 (Iron Chelating Agents); 3352-57-6 (Hydroxyl Radical); 533-67-5 (Deoxyribose); BBX060AN9V (Hydrogen Peroxide); E1UOL152H7 (Iron)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2016_1385


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[PMID]:27825024
[Au] Autor:Brizzolari A; Campisi GM; Santaniello E; Razzaghi-Asl N; Saso L; Foti MC
[Ad] Endereço:Dipartimento di Scienze della Salute, Università degli Studi di Milano, c/o Ospedale S. Paolo, Via A. di Rudinì, 8-20142 Milano, Italy.
[Ti] Título:Effect of organic co-solvents in the evaluation of the hydroxyl radical scavenging activity by the 2-deoxyribose degradation assay: The paradigmatic case of α-lipoic acid.
[So] Source:Biophys Chem;220:1-6, 2017 Jan.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The 2-deoxyribose degradation assay (2-DR assay) is an in vitro method broadly used for evaluating the scavenging activity against the hydroxyl radical (HO). One of the major drawbacks of the assay, however, is that only water soluble compounds can be tested for their radical-scavenging activity. Lipoic acid (LA) is an excellent scavenger of HO but it exhibits a low solubility in the aqueous milieu of the 2-DR assay and a high tendency to polymerize under a variety of conditions. We used LA as a paradigmatic substrate to evaluate the effect of several organic co-solvents in increasing its solubility. Most of these solvents, however, demonstrated to be potent scavengers of HO making their use in the 2-DR assay improper. On the other hand, acetonitrile showed a remarkably low reactivity toward HO (rate constant ~8.7×10 M s ) which allowed us to use it as a co-solvent in the preparation of stock solutions of LA ~5mM. We therefore evaluated the radical-scavenging activity of LA by the 2-DR assay in a relatively large range of concentrations, 1-200µM. We found that the rate constant for LA+HO is diffusion-controlled (~1×10 M s in water at 25°C) and uninfluenced by the presence of small quantities of acetonitrile. Therefore, the use of acetonitrile in the 2-DR assay does not interfere with the test and may increase the solubility of the radical scavengers.
[Mh] Termos MeSH primário: Desoxirribose/química
Depuradores de Radicais Livres/química
Radical Hidroxila/química
Solventes/química
Ácido Tióctico/química
[Mh] Termos MeSH secundário: Acetonitrilos
Compostos Orgânicos/química
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Free Radical Scavengers); 0 (Organic Chemicals); 0 (Solvents); 3352-57-6 (Hydroxyl Radical); 533-67-5 (Deoxyribose); 73Y7P0K73Y (Thioctic Acid); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


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[PMID]:28042856
[Au] Autor:de Graft-Johnson J; Nowak D
[Ad] Endereço:Heart and Vascular Institute of North Florida, 2623 Centennial Blvd., Suite 102, Tallahassee, FL 32308, USA. adegraft@umich.edu.
[Ti] Título:Effect of Selected Plant Phenolics on Fe -EDTA-H2O2 System Mediated Deoxyribose Oxidation: Molecular Structure-Derived Relationships of Anti- and Pro-Oxidant Actions.
[So] Source:Molecules;22(1), 2016 Dec 31.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In the presence of transition metal ions and peroxides, polyphenols, well-known dietary antioxidants, can act as pro-oxidants. We investigated the effect of 13 polyphenols and their metabolites on oxidative degradation of deoxyribose by an OH generating Fenton system (Fe -ethylenediaminetetraacetic acid (EDTA)-H2O2). The relationship between phenolics pro-oxidant/anti-oxidant effects and their molecular structure was analyzed using multivariate analysis with multiple linear regression and a backward stepwise technique. Four phenolics revealed a significant inhibitory effect on OH-induced deoxyribose degradation, ranging from 54.4% ± 28.6% (3,4-dihydroxycinnamic acid) to 38.5% ± 10.4% (catechin) ( = 6), correlating with the number of -OH substitutions ( = 0.58). Seven phenolics augmented the oxidative degradation of deoxyribose with the highest enhancement at 95.0% ± 21.3% (quercetin) and 60.6% ± 12.2% (phloridzin). The pro-oxidant effect correlated ( < 0.05) with the number of -OH groups ( = 0.59), and aliphatic substitutes ( = -0.22) and weakly correlated with the occurrence of a catechol structure within the compound molecule ( = 0.17). Selective dietary supplementation with phenolics exhibiting pro-oxidant activity may increase the possibility of systemic oxidative stress in patients treated with medications containing chelating properties or those with high plasma concentrations of H2O2 and non-transferrin bound iron.
[Mh] Termos MeSH primário: Antioxidantes/química
Desoxirribose/metabolismo
Ácido Edético/química
Oxidantes/química
Fenóis/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Desoxirribose/química
Frutas/química
Seres Humanos
Peróxido de Hidrogênio/química
Oxirredução
Estresse Oxidativo
Verduras/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Oxidants); 0 (Phenols); 0 (Plant Extracts); 533-67-5 (Deoxyribose); 9G34HU7RV0 (Edetic Acid); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE


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[PMID]:27594348
[Au] Autor:Rideout MC; Liet B; Gasparutto D; Berthet N
[Ad] Endereço:Département de Chimie Moléculaire (DCM), Laboratoire Ingénierie et Interactions BioMoléculaires (I2BM), UMR-5250, ICMG FR-2607, CNRS, Université Grenoble Alpes (UGA), 570 Rue de la Chimie, BP-53, 38041 Grenoble Cedex 9, France. Electronic address: rideout.marc@gmail.com.
[Ti] Título:A high-throughput screen for detection of compound-dependent phosphodiester bond cleavage at abasic sites.
[So] Source:Anal Biochem;513:93-97, 2016 Nov 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have employed a DNA molecular beacon with a real abasic site, namely a 2-deoxyribose, in a fluorescent high-throughput assay to identify artificial nucleases that cleave at abasic sites. We screened a 1280 compound chemical library and identified a compound that functions as an artificial nuclease. We validated a key structure-activity relationship necessary for abasic site cleavage using available analogs of the identified artificial nuclease. We also addressed the activity of the identified compound with dose titrations in the absence and presence of a source of non-specific DNA. Finally, we characterized the phosphodiester backbone cleavage at the abasic site using denaturing gel electrophoresis. This study provides a useful template for researchers seeking to rapidly identify new artificial nucleases.
[Mh] Termos MeSH primário: DNA/química
Desoxirribonucleases/química
Desoxirribose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
533-67-5 (Deoxyribose); 9007-49-2 (DNA); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE


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[PMID]:27581636
[Au] Autor:Nishioka T; Oshiro I; Onizuka K; Taniguchi Y; Sasaki S
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Kyushu University.
[Ti] Título:Efficient Thymidine-Selective DNA Interstrand Photo-activated Crosslinking by the 6-Thioguanine Connected via an Ethylene-Linker to the 2'-Deoxyribose Unit.
[So] Source:Chem Pharm Bull (Tokyo);64(9):1315-20, 2016.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Cross-linking is a widely-used technology in the studies of DNA, RNA and their complexes with proteins. Intrinsically active alkylating moieties and photo-activated agents are chemically or enzymatically incorporated into nucleic acids. Thionucleobases resemble the corresponding natural bases, and form cross-links by UVA irradiation. They form cross-links only with a site in close contact, thereby allowing identification of the contacts within the nucleic acids and/or between the nucleic acids and proteins in complex nucleoprotein assemblies. On the other hand, the thionucleobase forms a cross-link less efficiently for the reaction with the opposite natural base in the DNA duplex. In this study, 6-thioguanine was connected to 2'-deoxyribose through an ethylene linker at the 1'-position (Et-thioG). The linker was expected to bring the 6-thio group close to the nucleobase in the opposite strand. In a duplex in which the 2'-deoxy-6-thioguanosine (6-thio-dG) did not form a crosslink, Et-thioG efficiently formed crosslink with a high selectivity for T by UVA irradiation, but with a much lower efficiency for dA, dG, dC, 5-methyl-dC or dU. Interestingly, the yield of the photo-crosslinked product with dT was effectively improved in the presence of dithiothreitol or sodium hydrosulfide (NaSH) at a low UVA irradiation dose. The efficient and selective cross-link formation at a low UVA dose may be beneficial for the biological application of Et-thioG.
[Mh] Termos MeSH primário: Reagentes para Ligações Cruzadas/química
DNA/química
Desoxirribose/química
Etilenos/química
Tioguanina/química
Timidina/química
[Mh] Termos MeSH secundário: Reagentes para Ligações Cruzadas/síntese química
Processos Fotoquímicos
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Ethylenes); 533-67-5 (Deoxyribose); 9007-49-2 (DNA); 91GW059KN7 (ethylene); FTK8U1GZNX (Thioguanine); VC2W18DGKR (Thymidine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c16-00310


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[PMID]:27555494
[Au] Autor:Tulub AA
[Ad] Endereço:Argonne National Laboratory, 9700S, Argonne, IL, 60439, USA; St.Petersburg State University, Universitetskaya Nab. 7/9, 199034, St.Petersburg, Russia. Electronic address: atulub@yahoo.co.uk.
[Ti] Título:Magnesium cations assist with unpairing hydrogen-bonded 2-deoxyribose trinucleotides.
[So] Source:Arch Biochem Biophys;607:44-6, 2016 Oct 01.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:2-deoxyribose trinucleotides are essential units for storage and transfer of the genetic information. Nucleotide transpositions in trinucleotide sequences affect production of different amino acids. The study focuses on the mechanism of unpairing initially H-bonded trinucleotides. In living cells, the unpairing proceeds through DNA polymerase operating only in the presence of Mg cations. The DNA polymerase is a very complex system to be studied quantum chemically. In our simplistic approach, the polymerase is replaced by two Mg cations attached to both sides of the complementary trinucleotides. A distinguished feature of Mg in cell is in its easiness to accept and donate the electron density. In a particular molecular configuration, this makes Mg singly charged. As to the current case, we observe an unpaired electron on the Mg(+) and an unpaired electron on the trinucleotide - totally, a radical pair which coupling produces either triplet or singlet state. The study, based on the DFT B3LYP (6-311G** basis set) computations, shows that the singlet state energetically is less preferable than the triplet state. The latter is unstable and makes the trinucleotide strands unpair in the region where the singlet and triplet states cross.
[Mh] Termos MeSH primário: Desoxirribose/química
Magnésio/química
Nucleotídeos/química
[Mh] Termos MeSH secundário: Cátions
Simulação por Computador
DNA/química
DNA Polimerase Dirigida por DNA/química
Elétrons
Ligações de Hidrogênio
Modelos Moleculares
Conformação Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Nucleotides); 533-67-5 (Deoxyribose); 9007-49-2 (DNA); EC 2.7.7.7 (DNA-Directed DNA Polymerase); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE


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[PMID]:27538665
[Au] Autor:Formichi P; Radi E; Branca C; Battisti C; Brunetti J; Da Pozzo P; Giannini F; Dotti MT; Bracci L; Federico A
[Ad] Endereço:Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy.
[Ti] Título:Oxidative stress-induced apoptosis in peripheral blood lymphocytes from patients with POLG-related disorders.
[So] Source:J Neurol Sci;368:359-68, 2016 Sep 15.
[Is] ISSN:1878-5883
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: POLG-related disorders are a group of heterogeneous diseases characterized by an overlapping clinical presentations and associated with mutations in the POLG gene. POLG codes for the catalytic subunit of mitochondrial polymerase gamma (POLG), essential for mitochondrial DNA (mtDNA) replication and repair. Studies on mutator POLG mice showed an increase in oxidative stress and apoptosis. In this regard we analysed the involvement of POLG mutations in the apoptotic regulation, evaluating apoptosis in peripheral blood lymphocytes (PBLs) from patients with POLG-related diseases. METHODS: Cells were cultured under basal conditions and with 2-deoxy-d-ribose (dRib), a reducing sugar that induces apoptosis by oxidative stress. Apoptosis rate was assessed by flow cytometry. Phosphatidylserine translocation, mitochondrial membrane depolarization and caspase 3 activation were also analysed. RESULTS: Our data showed higher percentages of apoptosis after dRib treatment in patients with POLG mutations than in controls, while under basal culture conditions, apoptosis levels were similar in the two groups. CONCLUSIONS: Cells with POLG mutations are more sensitive than control cells to oxidative stress-induced apoptosis, confirming that mtDNA mutations may have a role in mitochondrial apoptosis pathway. We also suggest that redox state homeostasis may play a crucial role in phenotypic expression of POLG-related diseases.
[Mh] Termos MeSH primário: Apoptose/genética
DNA Polimerase Dirigida por DNA/genética
Doenças Genéticas Inatas/genética
Doenças Genéticas Inatas/patologia
Linfócitos/patologia
Mutação/genética
Estresse Oxidativo/fisiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Estudos de Casos e Controles
Caspase 3
Células Cultivadas
DNA Polimerase gama
Desoxirribose/farmacologia
Feminino
Citometria de Fluxo
Seres Humanos
Linfócitos/metabolismo
Masculino
Potencial da Membrana Mitocondrial/genética
Meia-Idade
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
533-67-5 (Deoxyribose); EC 2.7.7.7 (DNA Polymerase gamma); EC 2.7.7.7 (DNA-Directed DNA Polymerase); EC 2.7.7.7 (POLG protein, human); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE


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[PMID]:27471832
[Au] Autor:Pieczykolan M; Staszewska-Krajewska O; Furman B; Chmielewski M
[Ad] Endereço:Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
[Ti] Título:1,3-Dipolar cycloaddition of a cyclic nitrone derived from 2-deoxy-D-ribose to α,ß-unsaturated lactones: An entry to carbapenem antibiotics.
[So] Source:Carbohydr Res;433:89-96, 2016 Oct 04.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:1,3-Dipolar cycloadditions of 2-deoxy-D-ribose-derived L-threo five-membered cyclic nitrone to α,ß-unsaturated γ- and δ-lactones were investigated. Cycloadducts obtained from δ-lactones, after NO bond cleavage, opening of the lactone ring, and protection of hydroxyl groups were subjected to ß-lactam ring formation by using Mukaiyama's salt. Cycloadducts from γ-lactones subjected to the same reaction sequence undergo ß-elimination of a water molecule to provide pyrrolidine-substituted unsaturated γ-lactones.
[Mh] Termos MeSH primário: Lactonas/química
Óxidos de Nitrogênio/química
beta-Lactamas/síntese química
[Mh] Termos MeSH secundário: Carbapenêmicos/síntese química
Carbapenêmicos/química
Reação de Cicloadição
Desoxirribose/química
Estrutura Molecular
Estereoisomerismo
beta-Lactamas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbapenems); 0 (Lactones); 0 (Nitrogen Oxides); 0 (beta-Lactams); 0 (nitrones); 533-67-5 (Deoxyribose)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE


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[PMID]:27264558
[Au] Autor:Quiñones JL; Demple B
[Ad] Endereço:Stony Brook University School of Medicine, Department of Pharmacological Sciences, Stony Brook, NY, 11794, USA.
[Ti] Título:When DNA repair goes wrong: BER-generated DNA-protein crosslinks to oxidative lesions.
[So] Source:DNA Repair (Amst);44:103-109, 2016 Aug.
[Is] ISSN:1568-7856
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Free radicals generate an array of DNA lesions affecting all parts of the molecule. The damage to deoxyribose receives less attention than base damage, even though the former accounts for ∼20% of the total. Oxidative deoxyribose fragments (e.g., 3'-phosphoglycolate esters) are removed by the Ape1 AP endonuclease and other enzymes in mammalian cells to enable DNA repair synthesis. Oxidized abasic sites are initially incised by Ape1, thus recruiting these lesions into base excision repair (BER) pathways. Lesions such as 2-deoxypentos-4-ulose can be removed by conventional (single-nucleotide) BER, which proceeds through a covalent Schiff base intermediate with DNA polymerase ß (Polß) that is resolved by hydrolysis. In contrast, the lesion 2-deoxyribonolactone (dL) must be processed by multinucleotide ("long-patch") BER: attempted repair via the single-nucleotide pathway leads to a dead-end, covalent complex with Polß cross- linked to the DNA by an amide bond. We recently detected these stable DNA-protein crosslinks (DPC) between Polß and dL in intact cells. The features of the DPC formation in vivo are exactly in keeping with the mechanistic properties seen in vitro: Polß-DPC are formed by oxidative agents in line with their ability to form the dL lesion; they are not formed by non-oxidative agents; DPC formation absolutely requires the active-site lysine-72 that attacks the 5'-deoxyribose; and DPC formation depends on Ape1 to incise the dL lesion first. The Polß-DPC are rapidly processed in vivo, the signal disappearing with a half-life of 15-30min in both mouse and human cells. This removal is blocked by inhibiting the proteasome, which leads to the accumulation of ubiquitin associated with the Polß-DPC. While other proteins (e.g., topoisomerases) also form DPC under these conditions, 60-70% of the trapped ubiquitin depends on Polß. The mechanism of ubiquitin targeting to Polß-DPC, the subsequent processing of the expected 5'-peptidyl-dL, and the biological consequences of unrepaired DPC are important to assess. Many other lyase enzymes that attack dL can also be trapped in DPC, so the processing mechanisms may apply quite broadly.
[Mh] Termos MeSH primário: DNA Polimerase beta/metabolismo
Reparo do DNA
DNA Liase (Sítios Apurínicos ou Apirimidínicos)/metabolismo
DNA/metabolismo
Desoxirribose/metabolismo
[Mh] Termos MeSH secundário: Animais
Dano ao DNA
DNA Polimerase beta/genética
DNA Liase (Sítios Apurínicos ou Apirimidínicos)/genética
Desoxirribose/química
Meia-Vida
Seres Humanos
Cetoses/metabolismo
Cinética
Camundongos
Estresse Oxidativo
Complexo de Endopeptidases do Proteassoma/metabolismo
Ligação Proteica
Proteólise
Açúcares Ácidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Ketoses); 0 (Sugar Acids); 34371-14-7 (2,4,5-trihydroxypentanoic acid gamma-lactone); 533-67-5 (Deoxyribose); 9007-49-2 (DNA); EC 2.7.7.- (DNA Polymerase beta); EC 3.4.25.1 (Proteasome Endopeptidase Complex); EC 4.2.99.18 (APEX1 protein, human); EC 4.2.99.18 (DNA-(Apurinic or Apyrimidinic Site) Lyase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160607
[St] Status:MEDLINE



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