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| [PMID]: | 29247645 |
| [Au] Autor: | Qin C; Yang L; Zheng W; Yan X; Lu R; Xie D; Nie G |
| [Ad] Endereço: | Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, No. 46 Jianshe Road, Xinxiang 453007, PR China. |
| [Ti] Título: | Effects of dietary glucose and sodium chloride on intestinal glucose absorption of common carp (Cyprinus carpio L.). |
| [So] Source: | Biochem Biophys Res Commun;495(2):1948-1955, 2018 01 08. | | [Is] ISSN: | 1090-2104 |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | The co-transport of sodium and glucose is the first step for intestinal glucose absorption. Dietary glucose and sodium chloride (NaCl) may facilitate this physiological process in common carp (Cyprinus carpio L.). To test this hypothesis, we first investigated the feeding rhythm of intestinal glucose absorption. Carps were fed to satiety once a day (09:00 a.m.) for 1 month. Intestinal samples were collected at 01:00, 05:00, 09:00, 13:00, 17:00 and 21:00. Result showed that food intake greatly enhanced sodium/glucose cotransporter 1 (SGLT1) and glucose transporter type 2 (GLUT2) expressions, and improved glucose absorption, with highest levels at 09:00 a.m.. Then we designed iso-nitrogenous and iso-energetic diets with graded levels of glucose (10%, 20%, 30%, 40% and 50%) and NaCl (0%, 1%, 3% and 5%), and submitted to feeding trial for 10 weeks. The expressions of SGLT1 and GLUT2, brush border membrane vesicles (BBMVs) glucose transport and intestinal villus height were determined after the feeding trial. Increasing levels of dietary glucose and NaCl up-regulated mRNA and protein levels of SGLT1 and GLUT2, enhanced BBMVs glucose transport in the proximal, mid and distal intestine. As for histological adaptive response, however, high-glucose diet prolonged while high-NaCl diet shrank intestinal villus height. Furthermore, we also found that higher mRNA levels of SGLT1 and GLUT2, higher glucose transport capacity of BBMVs, and higher intestinal villus were detected in the proximal and mid intestine, compared to the distal part. Taken together, our study indicated that intestinal glucose absorption in carp was primarily occurred in the proximal and mid intestine, and increasing levels of dietary glucose and NaCl enhanced intestinal glucose absorption in carp. |
| [Mh] Termos MeSH primário: |
Carpas/metabolismo
Açúcares da Dieta/metabolismo
Ingestão de Alimentos/fisiologia
Glucose/metabolismo
Absorção Intestinal/fisiologia
Cloreto de Sódio na Dieta/metabolismo
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| [Mh] Termos MeSH secundário: |
Animais
Taxa de Depuração Metabólica
Distribuição Tecidual
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Dietary Sugars); 0 (Sodium Chloride, Dietary); IY9XDZ35W2 (Glucose) |
| [Em] Mês de entrada: | 1802 |
| [Cu] Atualização por classe: | 180214 |
[Lr] Data última revisão:
| 180214 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 171217 |
| [St] Status: | MEDLINE |
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