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[PMID]:29458566
[Au] Autor:Kim YS; Cha CJ
[Ad] Endereço:Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
[Ti] Título:Paenibacillus translucens sp. nov., isolated from tidal flat sediment.
[So] Source:Int J Syst Evol Microbiol;68(3):936-941, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11 , was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691 (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11 was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691 . The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11 contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11 (=KACC 19304 =JCM 32080 ).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Paenibacillus/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002613


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[PMID]:29458505
[Au] Autor:Yan ZF; Lin P; Li CT; Kook M; Yi TH
[Ad] Endereço:1​Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
[Ti] Título:Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea.
[So] Source:Int J Syst Evol Microbiol;68(3):788-794, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121 , was isolated from forest soil. Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121 were identified as Actinotalea ferrariae KCTC 29134 (97.9 %), Actinotalea fermentans KCTC 3251 (97.3 %), Cellulomonas carbonis KCTC 19824 (97.2 %). 16S rRNA gene sequence similarities among strain THG-T121 and other recognized species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid. The isoprenoid quinone was menaquinone (MK-10(H4)). The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The whole-cell sugars of strain THG-T121 were rhamnose, ribose, mannose and glucose. The peptidoglycan type of strain THG-T121 is A4ß, containing l-Orn-D-Ser-L-Asp. The DNA G+C content of strain THG-T121 was 72.4 mol%. DNA-DNA hybridization values between strain THG-T121 and A. ferrariae KCTC 29134 , A. fermentans KCTC 3251 and C. carbonis KCTC 19824 were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T121 represents a novel species of the genus Actinotalea, for which the name Actinotaleasolisilvae sp. nov. is proposed. The type strain is THG-T121 (=KACC 19191 =CGMCC 4.7389 ).
[Mh] Termos MeSH primário: Actinomycetales/classificação
Florestas
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Actinomycetales/genética
Actinomycetales/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002584


  3 / 10483 MEDLINE  
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[PMID]:29458462
[Au] Autor:Wang C; Huang Y; Li L; Guo J; Wu Z; Deng Y; Dai L; Ma S
[Ad] Endereço:1​Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
[Ti] Título:Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread.
[So] Source:Int J Syst Evol Microbiol;68(3):703-708, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus, designated strain Bb 2-3 , was isolated from bee bread of Apis cerana collected from a hive in Kunming, China. The strain was regular rod-shaped. Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l NaCl. The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso. Respiratory quinones were not detected. Seven glycolipids, three lipids, phosphatidylglycerol and diphosphatidylglycerol were detected. The peptidoglycan type A4α l-Lys-d-Asp was determined. Strain Bb 2-3 was closely related to Lactobacillus bombicola DSM 28793 , Lactobacillus apis LMG 26964 and Lactobacillus helsingborgensis DSM 26265 , with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively. A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3 was well separated from the reference strains of species of the genus Lactobacillus. The average nucleotide identity between strain Bb 2-3 and the type strains of closely related species was lower than the 95-96 % threshold value for delineation of genomic prokaryotic species. The G+C content of the genomic DNA of strain Bb 2-3 was 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3 is proposed to represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus panisapium sp. nov. The type strain is Bb 2-3 (=DSM 102188 =ACCC 19955 ).
[Mh] Termos MeSH primário: Abelhas/microbiologia
Lactobacillus/classificação
Filogenia
Própole
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Lactobacillus/genética
Lactobacillus/isolamento & purificação
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 9009-62-5 (Propolis)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002538


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[PMID]:29184406
[Au] Autor:Yuan M; Ding S; Meng T; Lu B; Shao S; Zhang X; Yuan H; Hu F
[Ad] Endereço:Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan.
[Ti] Título:Effect of A-317491 delivered by glycolipid-like polymer micelles on endometriosis pain.
[So] Source:Int J Nanomedicine;12:8171-8183, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Endometriosis is a common gynecological disease with a lack of effective clinical treatment. Current therapy often results in endometriosis pain recurrence and serious side effects. P2X receptor, an adenosine triphosphate (ATP)-gated ion channel, might be implicated in endometriosis pain. In this study, chitosan oligosaccharide-g-stearic acid (CSOSA) polymer micelles-coated nanostructured lipid carriers (NLCs) were developed as a novel delivery system for A-317491, a selective P2X receptor antagonist for endometriosis pain therapy. A-317491-loaded NLC (NLC/A-317491) could be coated by CSOSA micelles to form CSOSA/NLC/A-317491 nanoparticles. Pheochromocytoma PC12 cells, which highly expressed P2X receptors, were used as a cell model, and the CSOSA/NLC/A-317491 partly blocked the Ca influx induced by ATP stimulation. In nude mouse and rat endometriotic models, CSOSA/NLC could accumulate into endometriotic lesions after vein injection. In endometriotic rats, CSOSA/NLC/A-317491 reversed mechanical and heat hyperalgesia with long-term efficacy, which might be attributed to the massive CSOSA/NLC/A-317491 distribution in the endometriotic lesions. In conclusion, A-317491 delivered by CSOSA/NLC nanoparticles attenuated endometriosis pain in rats, and CSOSA/NLC/A-317491 could be used as an effective treatment strategy for P2X -targeted therapy in endometriosis pain.
[Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos/métodos
Endometriose/tratamento farmacológico
Nanopartículas/administração & dosagem
Dor/tratamento farmacológico
Fenóis/administração & dosagem
Compostos Policíclicos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Feminino
Glicolipídeos/química
Seres Humanos
Camundongos Nus
Micelas
Nanopartículas/química
Oligossacarídeos/química
Células PC12
Fenóis/química
Fenóis/farmacologia
Compostos Policíclicos/química
Compostos Policíclicos/farmacologia
Polímeros/química
Antagonistas do Receptor Purinérgico P2X/farmacologia
Ratos
Receptores Purinérgicos P2X3
Ácidos Esteáricos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A-317491); 0 (Glycolipids); 0 (Micelles); 0 (Oligosaccharides); 0 (Phenols); 0 (Polycyclic Compounds); 0 (Polymers); 0 (Purinergic P2X Receptor Antagonists); 0 (Receptors, Purinergic P2X3); 0 (Stearic Acids); 4ELV7Z65AP (stearic acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146569


  5 / 10483 MEDLINE  
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[PMID]:29311511
[Au] Autor:Sakurai K
[Ad] Endereço:Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology.
[Ti] Título:A Peptide-Glycolipid Interaction Probed by Retroinverso Peptide Analogues.
[So] Source:Chem Pharm Bull (Tokyo);66(1):45-50, 2018.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Cell surface glycolipids are implicated in the formation of lipid rafts and membrane microdomains, where they interact with protein receptors to mediate a variety of cellular processes such as cell-cell recognition, cell adhesion, and membrane signaling. Studies of glycolipid function at the local membrane structures have not been straightforward to date, because the locally clustered structures are labile and their protein binding affinities tend to be weak. While specific glycolipid-binding proteins have been employed as molecular probes for detecting lipid rafts, small peptides may be more suitable for probing glycolipids at the cell surface due to their small size as well as their ease of synthetic preparation and functionalization. Here we report an application of the retroinverso approach as a rapid method to obtain novel glycolipid-binding D-peptide sequences. We have prepared analogues of two known GM1-binding peptides by replacing L-amino acids with D-amino acids, followed by inverting the sequences and characterized their conformational propensity and glycolipid binding properties. Circular dichroism (CD) spectroscopic analysis indicated that all the peptide sequences interacted with GM1 under a micellar condition. We found, by a microplate-based competitive glycolipid binding assay, that one of the retroinverso D-peptide analogues, peptide 3, also binds GM1 as the parent L-peptide 1. These results suggested that in this glycolipid-peptide interaction, the positioning of the side chain functionalities of the peptide is important, while the peptide backbone polarity is not. Glycolipid binding retroinverso D-peptides should be useful for the design of new peptide-based probes for investigating the biological role of cell surface glycolipids.
[Mh] Termos MeSH primário: Glicolipídeos/química
Peptídeos/química
[Mh] Termos MeSH secundário: Conformação Molecular
Peptídeos/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolipids); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00455


  6 / 10483 MEDLINE  
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[PMID]:28467902
[Au] Autor:Furukawa A; Yoshikaie K; Mori T; Mori H; Morimoto YV; Sugano Y; Iwaki S; Minamino T; Sugita Y; Tanaka Y; Tsukazaki T
[Ad] Endereço:Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
[Ti] Título:Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF.
[So] Source:Cell Rep;19(5):895-901, 2017 May 02.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein secretion mediated by SecYEG translocon and SecA ATPase is enhanced by membrane-embedded SecDF by using proton motive force. A previous structural study of SecDF indicated that it comprises 12 transmembrane helices that can conduct protons and three periplasmic domains, which form at least two characterized transition states, termed the F and I forms. We report the structures of full-length SecDF in I form at 2.6- to 2.8-Å resolution. The structures revealed that SecDF in I form can generate a tunnel that penetrates the transmembrane region and functions as a proton pathway regulated by a conserved Asp residue of the transmembrane region. In one crystal structure, periplasmic cavity interacts with a molecule, potentially polyethylene glycol, which may mimic a substrate peptide. This study provides structural insights into the Sec protein translocation that allows future analyses to develop a more detailed working model for SecDF.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Prótons
Canais de Translocação SEC/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Sítios de Ligação
Membrana Celular/química
Membrana Celular/metabolismo
Cristalografia por Raios X
Deinococcus/química
Glicolipídeos
Peptídeos/química
Peptídeos/metabolismo
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Glycolipids); 0 (Peptides); 0 (Protons); 0 (SEC Translocation Channels); 0 (deinococcucin A)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29388549
[Au] Autor:Yan ZF; Lin P; Won KH; Li CT; Park G; Chin B; Kook M; Wang QJ; Yi TH
[Ad] Endereço:1​College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
[Ti] Título:Sphingomonas rhizophila sp. nov., isolated from rhizosphere of Hibiscus syriacus.
[So] Source:Int J Syst Evol Microbiol;68(2):681-686, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61 ), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61 were identified as Sphingomonas ginsengisoli KCTC 12630 (97.9 %), Sphingomonas jaspsi DSM 18422 (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146 (97.4 %), Sphingomonassediminicola KCTC 12629 (97.2 %), 'Sphingomonas swuensis' KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718 (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1ω7c, C18 : 1ω9t and/or C18 : 1ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61 was 65.6 mol%. The DNA-DNA relatedness values between strain THG-T61 and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61 represents a novel species of the genus Sphingomonas, for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61 (=KACC 19189 =CCTCC AB 2016245 ).
[Mh] Termos MeSH primário: Hibiscus/microbiologia
Filogenia
Rizosfera
Microbiologia do Solo
Sphingomonas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Espermidina/química
Sphingomonas/genética
Sphingomonas/isolamento & purificação
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002566


  8 / 10483 MEDLINE  
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[PMID]:29388546
[Au] Autor:Subhash Y; Lee SS
[Ad] Endereço:Department of Life Science, College of Natural Science, Kyonggi University, 154-42 Gwanggyosan-ro Yeongtong-gu Suwon-Si, Gyeonggi-Do, 16227, Republic of Korea.
[Ti] Título:Roseomonas deserti sp. nov., isolated from crude oil contaminated desert sand.
[So] Source:Int J Syst Evol Microbiol;68(2):675-680, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two dark pink pigmented bacterial strains (M3 and M11) were isolated from crude oil contaminated desert sand from Kuwait. Both strains were Gram-stain-negative and small-rod to oval-shaped bacteria. Strains M3 and M11 grew at 13-42 °C (optimum, 30-35 °C) and pH 6.5-9.0 (optimum, 7.0-7.5). No additional NaCl was required for the growth of both strains. The genomic DNA G+C content of strains M3 and M11 were 69.5 and 69.0 mol%, respectively. Both strains were closely related and the mean DNA-DNA hybridization value was 92±1 %. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus Roseomonas. Strains M3 and M11 had a sequence similarity of 97.3 and 97.4 % with Roseomonas oryzae JC288 , respectively. Both strains had <97 % 16S rRNA gene sequence similarity with other members of the genus Roseomonas. Strain M3 showed 18±2 and 13±2 % reassociation (based on DNA-DNA hybridization) with R. oryzae KCTC 42542 and Roseomonas cervicalis KACC 11686 , respectively. The major cellular fatty acids (>5 %) were identified as C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c and C16 : 0 in both strains. Both strains showed diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidylcholine and unidentified glycolipid as major polar lipids. Based on distinct phenotypic, genotypic and phylogenetic differences from the previously described taxa, we propose the classification of strains M3 and M11 as representative of a novel species in the genus Roseomonas, for which the name Roseomonas deserti sp. nov. is suggested. The type strain is M3 (=KEMB 2255-459 =JCM 31275 ).
[Mh] Termos MeSH primário: Clima Desértico
Methylobacteriaceae/classificação
Petróleo/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Kuweit
Methylobacteriaceae/genética
Methylobacteriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Dióxido de Silício
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Petroleum); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002565


  9 / 10483 MEDLINE  
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[PMID]:29388545
[Au] Autor:Wu H; Liu B; Shao Y; Ou X; Huang F
[Ad] Endereço:Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning, PR China.
[Ti] Título:Thermostaphylospora grisealba gen. nov., sp. nov., isolated from mushroom compost and transfer of Thermomonospora chromogena Zhang et al. 1998 to Thermostaphylospora chromogena comb. nov.
[So] Source:Int J Syst Evol Microbiol;68(2):602-608, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel thermophilic actinomycete, designated strain 3-12X , was isolated from mushroom compost in Guangxi University, Nanning, China. The novel isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were glucose and ribose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The polar phospholipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, ninhydrin-positive phosphoglycolipids and glycolipids. Major fatty acids were so-C16 : 0 and C17 : 0. The G+C content of the genomic DNA was 74.6 %. The 16S rRNA gene sequence analysis showed that the closest phylogenetic neighbour of strain 3-12X was Thermomonospora chromogena ATCC 43196 (97.0 %), other closely related strains all belonged to the family Streptosporangiaceae and showed more than 6 % divergence. The chemotaxonomic characteristics of strain 3-12X were significantly different from Thermomonospora chromogena ATCC 43196 and DNA-DNA hybridization showed low relatedness (48.6-55.6 %) between them, so they should be different species. Thermomonospora chromogena was removed from the genus Thermomonospora by Zhang et al. 1998 on the basis of phylogenetic, chemotaxonomic and phenotypic evidence, but its taxonomic position remains uncertain. Based on the phenotypic and phylogenetic data, strain 3-12X represents a novel species in a new genus in the family Streptosporangiaceae. The name Thermostaphylospora griseoalba gen. nov., sp. nov. is proposed. The type strain of Thermostaphylospora grisealba is 3-12X (=DSM 46781 =CGMCC 4.7160 ). We also propose transferring Thermomonospora chromogenaZhang et al. 1998 to Thermostaphylospora chromogena comb. nov. (type strain ATCC 43196 =JCM 6244 ).
[Mh] Termos MeSH primário: Actinomycetales/classificação
Agaricales
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Actinomycetales/genética
Actinomycetales/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
Compostagem
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002551


  10 / 10483 MEDLINE  
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[PMID]:29265998
[Au] Autor:Li Y; Xu G; Lin C; Wang X; Piao CG
[Ad] Endereço:The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
[Ti] Título:Aureimonas populi sp. nov., isolated from poplar tree bark.
[So] Source:Int J Syst Evol Microbiol;68(2):487-491, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two novel bacterial strains (4M3-2 and 10-107-7) were isolated from poplar tree bark. The strains were Gram-stain-negative facultative aerobes, and produced short rods that were motile because of polar flagella. A phylogenetic tree was reconstructed based on 16S rRNA gene sequences indicating that the two novel strains are related to species of the genus Aureimonas and Aurantimonas. The two novel strains shared the highest 16S rRNA gene sequence similarities with Aureimonasfrigidaquae CW5 7Y-4 (97.1 %) and Aureimonasaltamirensis DSM 21988 (96.6 %)o. The lipids of the novel strain contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and sulfoquinovosyl diacylglycerol. The presence of a distinct glycolipid (sulfoquinovosyl diacylglycerol) is an important chemotaxonomic feature used to distinguish between species of the genera, Aurantimonas and Aureimonas. Additionally, the DNA-DNA hybridization results indicated that the two novel strains represent a novel taxon distinct from Aureimonas frigidaquae. The results of the 16S rRNA gene sequence analysis, as well as the physiological and biochemical characteristics imply that the two novel strains should be assigned to a novel species, with the proposed name Aureimonas populi sp. nov. The type strain is 4M3-2 (=CFCC 11187 =KCTC 42087 ).
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Filogenia
Casca de Planta/microbiologia
Populus/microbiologia
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Glicolipídeos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002479



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