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[PMID]:28454589
[Au] Autor:Beyene T; Hayishe H; Gizaw F; Beyi AF; Abunna F; Mammo B; Ayana D; Waktole H; Abdi RD
[Ad] Endereço:Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia. takele.beyene@aau.edu.et.
[Ti] Título:Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia.
[So] Source:BMC Res Notes;10(1):171, 2017 Apr 28.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Staphylococcus species cause mastitis and wound infection in livestock and food poisoning in humans through ingestion of contaminated foods, including meat and dairy products. They are evolving pathogens in that they readily acquire drug resistance, and multiple drug-resistant (MDR) isolates are increasing in human and veterinary healthcare. Therefore, this study was conducted to evaluate the prevalence of Staphylococci and their drug resistance in dairy farms and abattoir settings of Addis Ababa. METHODS: In this cross-sectional study, 193 samples of milk, meat, equipment and humans working in the dairy farms and abattoir were collected (dairy farms = 72 and abattoir sources = 121). Staphylococcus isolation and identification at the species level was done according to ISO-6888-3 using biochemical characteristics. An antimicrobial susceptibility test was conducted for 43 of the isolates using 15 antimicrobial agents commonly used for humans and livestock by the Kirby Bauer disk diffusion method following CLSI guidelines. RESULTS: Staphylococcus organism were isolated from 92 (47.7%) of the total 193 samples, 50% in the dairy farms and 46.3% in the abattoir. The isolated species were S. aureus (n = 31; 16.1%), S. intermedius (n = 21; 10.9%), S. hyicus (n = 16; 8.3%), and coagulase negative Staphylococcus (CNS) (n = 24; 12.4%). Gentamycin was effective drug as all isolates (n = 43; 100%) were susceptible to it and followed by kanamycin (n = 39; 90.7%). However, the majority of the isolates showed resistance to penicillin-G (95.3%), nalidixic acid (88.4%), cloxacillin (79.1%), vancomycin (65.1%) and cefoxitin (55.8%). Of the 15 S. aureus tested for drug susceptibility, 73.3% of them were phenotypically resistant to vancomycin (VRSA) and all of the 15 isolates showed multi-drug resistance (MDR) to >3 drugs. Also, all of the tested CNS (100%), S. hyicus (100%) and the majority of S. intermedius isolates (88.9%) developed MDR. CONCLUSION: Alarmingly, the Staphylococcus isolates circulating in the dairy farms and abattoir in the study area harbor MDR. High level of Staphylococcus species isolation from personnel and equipment besides food (meat and milk) samples in dairy farms and abattoir settings reveals that the hygiene practice in the dairy farm and abattoir is substandard. Prudent drug use and improved hygienic practice is recommended in the dairy farms and abattoir to safeguard the public from the risk of acquiring infections and MDR pathogenic Staphylococcus.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana/genética
Mastite Bovina/epidemiologia
Staphylococcus aureus Resistente à Meticilina/genética
Saúde do Trabalhador/educação
Infecções Estafilocócicas/epidemiologia
Staphylococcus/genética
[Mh] Termos MeSH secundário: Matadouros
Criação de Animais Domésticos
Animais
Antibacterianos/farmacologia
Técnicas de Tipagem Bacteriana
Bovinos
Estudos Transversais
Etiópia/epidemiologia
Fazendas
Feminino
Gentamicinas/farmacologia
Seres Humanos
Canamicina/farmacologia
Mastite Bovina/microbiologia
Mastite Bovina/transmissão
Carne/microbiologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Leite/microbiologia
Prevalência
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/transmissão
Staphylococcus/classificação
Staphylococcus/efeitos dos fármacos
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-017-2487-y


  2 / 17370 MEDLINE  
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[PMID]:27777409
[Au] Autor:El-Chaar GM; Supaswud-Franks T; Venugopalan L; Kohn N; Castro-Alcaraz S
[Ad] Endereço:Clinical Health Professions, St. John's University, Jamaica, USA.
[Ti] Título:Response to "Extended-interval gentamicin administration in neonates: an over-simplified approach".
[So] Source:J Perinatol;36(11):1028, 2016 11.
[Is] ISSN:1476-5543
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antibacterianos
Gentamicinas
[Mh] Termos MeSH secundário: Seres Humanos
Recém-Nascido
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1038/jp.2016.148


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[PMID]:27777408
[Au] Autor:Dersch-Mills D; AlShaikh B; Akierman A; Yusuf K
[Ad] Endereço:Department of Medicine, Alberta Health Services, Calgary, AB, Canada.
[Ti] Título:Extended-interval gentamicin administration in neonates: an over-simplified approach.
[So] Source:J Perinatol;36(11):1027, 2016 11.
[Is] ISSN:1476-5543
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antibacterianos
Gentamicinas
[Mh] Termos MeSH secundário: Seres Humanos
Recém-Nascido
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1038/jp.2016.141


  4 / 17370 MEDLINE  
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[PMID]:29187685
[Au] Autor:Kajikawa S; Oeda T; Park K; Yamamoto K; Sugiyama H; Sawada H
[Ad] Endereço:Department of Neurology, Utano Hospital, National Hospital Organization.
[Ti] Título:[A case of subarachnoid hemorrhage due to infective endocarditis by methicillin-resistant coagulase-negative staphylococcus].
[So] Source:Rinsho Shinkeigaku;57(12):775-777, 2017 Dec 27.
[Is] ISSN:1882-0654
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:A 77-year-old man visited our hospital with unstable gait following 2 months of anorexia. Brain MRI showed multiple infarcts; cardiac echocardiography revealed mitral-valve vegetation; and blood culture revealed methicillin-resistant coagulase-negative staphylococci. The patient was diagnosed with infective endocarditis (IE). Subarachnoid hemorrhage (SAH) developed ten days after antibiotic treatment. Intracranial aneurysm was not found. We speculated that chronic inflammation of the cerebral arterial walls by bacteria of low virulence was associated with SAH complication. The vegetation disappeared following additional gentamicin administration and the patient recovered to walk.
[Mh] Termos MeSH primário: Endocardite/complicações
Endocardite/microbiologia
Staphylococcus aureus Resistente à Meticilina
Infecções Estafilocócicas
Hemorragia Subaracnóidea/etiologia
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/administração & dosagem
Ceftriaxona/administração & dosagem
Ecocardiografia Transesofagiana
Endocardite/diagnóstico por imagem
Endocardite/tratamento farmacológico
Gentamicinas/administração & dosagem
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Hemorragia Subaracnóidea/diagnóstico por imagem
Tomografia Computadorizada por Raios X
Resultado do Tratamento
Vancomicina/administração & dosagem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 6Q205EH1VU (Vancomycin); 75J73V1629 (Ceftriaxone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.5692/clinicalneurol.cn-001053


  5 / 17370 MEDLINE  
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[PMID]:28450199
[Au] Autor:Calin R; Caumes E; Reibel F; Ali Mohamed A; Brossier F; Foltz V; Boussouar S; Fautrel B; Maurin M; Katlama C; Pourcher V
[Ad] Endereço:Infectious and Tropical Diseases Department, Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris, Pierre et Marie Curie University, Paris, France. Electronic address: ruxandra.calin@aphp.fr.
[Ti] Título:Severe glandular tularemia in a patient treated with anti-tumour necrosis factor for psoriatic arthritis.
[So] Source:Int J Infect Dis;60:1-3, 2017 Jul.
[Is] ISSN:1878-3511
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:A case of severe glandular tularemia in a patient receiving anti-tumour necrosis factor (TNF) therapy is reported here. The patient required prolonged treatment with doxycycline-ciprofloxacin due to early relapse after ciprofloxacin was stopped. Tularemia may have a more severe course in patients receiving anti-TNF. This may thus be an indication for more aggressive treatment.
[Mh] Termos MeSH primário: Adalimumab/efeitos adversos
Antirreumáticos/efeitos adversos
Artrite Psoriásica/tratamento farmacológico
Tularemia/etiologia
Fator de Necrose Tumoral alfa/imunologia
[Mh] Termos MeSH secundário: Adalimumab/uso terapêutico
Adulto
Animais
Antibacterianos/uso terapêutico
Anticorpos Monoclonais/uso terapêutico
Antirreumáticos/uso terapêutico
Artrite Psoriásica/complicações
Gatos
Certolizumab Pegol/uso terapêutico
Ciprofloxacino/uso terapêutico
Cães
Doxiciclina/uso terapêutico
Fazendas
Feminino
França
Francisella tularensis/isolamento & purificação
Gentamicinas/uso terapêutico
Seres Humanos
Metotrexato/uso terapêutico
Coelhos
População Rural
Tularemia/induzido quimicamente
Tularemia/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antibodies, Monoclonal); 0 (Antirheumatic Agents); 0 (Gentamicins); 0 (Tumor Necrosis Factor-alpha); 5E8K9I0O4U (Ciprofloxacin); FYS6T7F842 (Adalimumab); N12000U13O (Doxycycline); UMD07X179E (Certolizumab Pegol); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 17370 MEDLINE  
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[PMID]:28988110
[Au] Autor:Fadel MA; El-Gebaly RH; Mohamed SA; Abdelbacki AMM
[Ad] Endereço:Biophysics Department, Faculty of Science, Cairo University, Egypt.
[Ti] Título:Biophysical control of the growth of Agrobacterium tumefaciens using extremely low frequency electromagnetic waves at resonance frequency.
[So] Source:Biochem Biophys Res Commun;494(1-2):365-371, 2017 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isolated Agrobacterium tumefaciens was exposed to different extremely low frequencies of square amplitude modulated waves (QAMW) from two generators to determine the resonance frequency that causes growth inhibition. The carrier was 10 MHz sine wave with amplitude ±10 Vpp which was modulated by a second wave generator with a modulation depth of ± 2Vpp and constant field strength of 200 V/m at 28 °C. The exposure of A. tumefaciens to 1.0 Hz QAMW for 90 min inhibited the bacterial growth by 49.2%. In addition, the tested antibiotics became more effective against A. tumefaciens after the exposure. Furthermore, results of DNA, dielectric relaxation and TEM showed highly significant molecular and morphological changes due to the exposure to 1.0 Hz QAMW for 90 min. An in-vivo study has been carried out on healthy tomato plants to test the pathogenicity of A. tumefaciens before and after the exposure to QAMW at the inhibiting frequency. Symptoms of crown gall and all pathological symptoms were more aggressive in tomato plants treated with non-exposed bacteria, comparing with those treated with exposed bacteria. We concluded that, the exposure of A. tumefaciens to 1.0 Hz QAMW for 90 min modified its cellular activity and DNA structure, which inhibited the growth and affected the microbe pathogenicity.
[Mh] Termos MeSH primário: Agrobacterium tumefaciens/efeitos da radiação
Antibacterianos/farmacologia
DNA Bacteriano/efeitos da radiação
Radiação Eletromagnética
[Mh] Termos MeSH secundário: Agrobacterium tumefaciens/efeitos dos fármacos
Agrobacterium tumefaciens/genética
Agrobacterium tumefaciens/crescimento & desenvolvimento
Amicacina/farmacologia
Carbenicilina/farmacologia
Cefaclor/farmacologia
Cloranfenicol/farmacologia
Ciprofloxacino/farmacologia
DNA Bacteriano/efeitos dos fármacos
Fluoroquinolonas/farmacologia
Gentamicinas/farmacologia
Lycopersicon esculentum/microbiologia
Tumores de Planta/microbiologia
Rifampina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Fluoroquinolones); 0 (Gentamicins); 5E8K9I0O4U (Ciprofloxacin); 66974FR9Q1 (Chloramphenicol); 69K7K19H4L (Cefaclor); 84319SGC3C (Amikacin); G42ZU72N5G (Carbenicillin); L4618BD7KJ (gatifloxacin); VJT6J7R4TR (Rifampin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  7 / 17370 MEDLINE  
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[PMID]:28916193
[Au] Autor:Gu L; Cui X; Wei W; Yang J; Li X
[Ad] Endereço:Department of Otolaryngology-Head and Neck Surgery, Qianfo Shan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China.
[Ti] Título:Ferulic acid promotes survival and differentiation of neural stem cells to prevent gentamicin-induced neuronal hearing loss.
[So] Source:Exp Cell Res;360(2):257-263, 2017 Nov 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neural stem cells (NSCs) have exhibited promising potential in therapies against neuronal hearing loss. Ferulic acid (FA) has been widely reported to enhance neurogenic differentiation of different stem cells. We investigated the role of FA in promoting NSC transplant therapy to prevent gentamicin-induced neuronal hearing loss. NSCs were isolated from mouse cochlear tissues to establish in vitro culture, which were then treated with FA. The survival and differentiation of NSCs were evaluated. Subsequently, neurite outgrowth and excitability of the in vitro neuronal network were assessed. Gentamicin was used to induce neuronal hearing loss in mice, in the presence and absence of FA, followed by assessments of auditory brainstem response (ABR) and distortion product optoacoustic emissions (DPOAE) amplitude. FA promoted survival, neurosphere formation and differentiation of NSCs, as well as neurite outgrowth and excitability of in vitro neuronal network. Furthermore, FA restored ABR threshold shifts and DPOAE in gentamicin-induced neuronal hearing loss mouse model in vivo. Our data, for the first time, support potential therapeutic efficacy of FA in promoting survival and differentiation of NSCs to prevent gentamicin-induced neuronal hearing loss.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Ácidos Cumáricos/farmacologia
Gentamicinas
Perda Auditiva/induzido quimicamente
Células-Tronco Neurais/efeitos dos fármacos
Células Receptoras Sensoriais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Cóclea/citologia
Cóclea/efeitos dos fármacos
Perda Auditiva/patologia
Camundongos
Camundongos Endogâmicos BALB C
Células-Tronco Neurais/fisiologia
Neurogênese/efeitos dos fármacos
Células Receptoras Sensoriais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumaric Acids); 0 (Gentamicins); AVM951ZWST (ferulic acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170917
[St] Status:MEDLINE


  8 / 17370 MEDLINE  
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[PMID]:28893358
[Au] Autor:Ko JH; Baek JY; Peck KR; Cho SY; Ha YE; Kim SH; Huh HJ; Lee NY; Kang CI; Chung DR; Song JH
[Ad] Endereço:1​Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Republic of Korea.
[Ti] Título:Discrepant susceptibility to gentamicin despite amikacin resistance in Klebsiella pneumoniae by VITEK 2 represents false susceptibility associated with the armA 16S rRNA methylase gene.
[So] Source:J Med Microbiol;66(10):1448-1450, 2017 Oct.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Because we experienced gentamicin failure in Klebsiella pneumoniae bacteraemia that was susceptible to gentamicin despite amikacin resistance, as determined by VITEK 2, we evaluated the true susceptibility and mechanism of resistance. We screened 2818 K. pneumoniae isolates during a 1-year period at a university hospital and reviewed anti-microbial susceptibility reports using the VITEK 2 system. The minimum inhibitory concentration was substantiated by broth microdilution (BMD), and the presence of 16S rRNA methylase genes and aminoglycoside-modifying enzymes was also investigated. A total of 131 amikacin-resistant isolates from 19 patients were gentamicin non-resistant according to the VITEK 2 system. Among these, we were able to collect isolates from 12 patients (63.2 %), and a single isolate from each patient was tested. Eleven of the gentamicin non-resistant isolates (91.7 %) showed high-level resistance to both amikacin and gentamicin by BMD in association with the armA gene. Gentamicin is not an adequate treatment option for amikacin-resistant K. pneumoniae, even if VITEK 2 reports susceptibility.
[Mh] Termos MeSH primário: Amicacina/farmacologia
Antibacterianos/farmacologia
Gentamicinas/farmacologia
Klebsiella pneumoniae/efeitos dos fármacos
Metiltransferases/metabolismo
[Mh] Termos MeSH secundário: Farmacorresistência Bacteriana Múltipla
Regulação Bacteriana da Expressão Gênica
Regulação Enzimológica da Expressão Gênica
Metiltransferases/genética
Testes de Sensibilidade Microbiana/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 84319SGC3C (Amikacin); EC 2.1.1.- (Methyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000583


  9 / 17370 MEDLINE  
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[PMID]:28847079
[Au] Autor:Liu Y; Feng Y; Cheng D; Xue J; Wakelin SA; Hu H; Li Z
[Ad] Endereço:Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, China.
[Ti] Título:Gentamicin degradation and changes in fungal diversity and physicochemical properties during composting of gentamicin production residue.
[So] Source:Bioresour Technol;244(Pt 1):905-912, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An indoor co-composting of gentamicin fermentation residues (GFR) and lovastatin fermentation residues (LFR) inoculated with gentamicin-degrading Aspergillus terreus FZC3 was conducted to remove gentamicin residues. The results showed that treatment MFZC3, consisting of a 10:1 blend of GFR and LFR (w/w), had the longest thermophilic phase (7days), quickest gentamicin degradation (t =4.4days), and relatively higher gentamicin degradation percentage (96.7%) at the end of composting. Addition of Aspergillus terreus FZC3 affected fungal diversity of the compost and improved the removal of gentamicin during composting of the 15:1 GFR:LFR blend. By analyzing the variations of gentamicin and fungal community dynamics, it was speculated that Aspergillus terreus could accelerate gentamicin degradation. The microbial community and dynamic during composting were deeply affected by the physicochemical properties, and vice versa. In conclusion, co-composting of GFR with LFR could be a promising technology to solve the problem of gentamicin residue in GFR waste.
[Mh] Termos MeSH primário: Gentamicinas
Solo
[Mh] Termos MeSH secundário: Fermentação
Fungos
Eliminação de Resíduos
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gentamicins); 0 (Soil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


  10 / 17370 MEDLINE  
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[PMID]:28813520
[Au] Autor:Khosravi AD; Motahar M; Abbasi Montazeri E
[Ad] Endereço:Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
[Ti] Título:The frequency of class1 and 2 integrons in Pseudomonas aeruginosa strains isolated from burn patients in a burn center of Ahvaz, Iran.
[So] Source:PLoS One;12(8):e0183061, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen with the ability to cause severe nosocomial infections and remains a major problem in burn patients. This organism shows a remarkable antimicrobial resistance and is often resistant to multiple antibiotics. Integron genes as mobile genetic elements are playing an important role in the spread of P. aeruginosa antibiotic resistance. This study was aimed to investigate the occurrence of class 1, and 2 integron genes (int1, int2), among P. aeruginosa strains isolated from patients with burn infections. METHODS: In total 93 clinical isolates of P. aeruginosa were screened. The antimicrobial susceptibilities of 9 common antimicrobial agents were tested against the isolates using disk diffusion method. PCR amplification was performed on extracted DNAs for the detection of int1, and int2 genes using the set of specific primers. RESULTS: The majority of P. aeruginosa isolates were from wound infection (69.9%). In disk diffusion method, most isolates showed remarkable resistance to tested antibiotics with highest against gentamicin (94.62%) and ciprofloxacin (93.55%). PCR amplification revealed that 89(95.7%) of P. aeruginosa strains carried int1, but none of them harbored int2 genes. The distribution of int1 gene was highest in blood (100%), followed by wound isolates (95.38%). CONCLUSIONS: We demonstrated a high antimicrobial resistance among P. aeruginosa isolates in our setting. int1 was prevalent and seems to play an important role in multidrug resistance among the isolates. So, performance of antibiotic surveillance programs is necessary for choosing the appropriate therapy and management of infection control practices.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Queimaduras/microbiologia
Integrons/fisiologia
Pseudomonas aeruginosa/isolamento & purificação
Pseudomonas aeruginosa/fisiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Unidades de Queimados/estatística & dados numéricos
Ciprofloxacino/farmacologia
Gentamicinas/farmacologia
Seres Humanos
Integrons/genética
Irã (Geográfico)
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Gentamicins); 5E8K9I0O4U (Ciprofloxacin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183061



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