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[PMID]:28454589
[Au] Autor:Beyene T; Hayishe H; Gizaw F; Beyi AF; Abunna F; Mammo B; Ayana D; Waktole H; Abdi RD
[Ad] Endereço:Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia. takele.beyene@aau.edu.et.
[Ti] Título:Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia.
[So] Source:BMC Res Notes;10(1):171, 2017 Apr 28.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Staphylococcus species cause mastitis and wound infection in livestock and food poisoning in humans through ingestion of contaminated foods, including meat and dairy products. They are evolving pathogens in that they readily acquire drug resistance, and multiple drug-resistant (MDR) isolates are increasing in human and veterinary healthcare. Therefore, this study was conducted to evaluate the prevalence of Staphylococci and their drug resistance in dairy farms and abattoir settings of Addis Ababa. METHODS: In this cross-sectional study, 193 samples of milk, meat, equipment and humans working in the dairy farms and abattoir were collected (dairy farms = 72 and abattoir sources = 121). Staphylococcus isolation and identification at the species level was done according to ISO-6888-3 using biochemical characteristics. An antimicrobial susceptibility test was conducted for 43 of the isolates using 15 antimicrobial agents commonly used for humans and livestock by the Kirby Bauer disk diffusion method following CLSI guidelines. RESULTS: Staphylococcus organism were isolated from 92 (47.7%) of the total 193 samples, 50% in the dairy farms and 46.3% in the abattoir. The isolated species were S. aureus (n = 31; 16.1%), S. intermedius (n = 21; 10.9%), S. hyicus (n = 16; 8.3%), and coagulase negative Staphylococcus (CNS) (n = 24; 12.4%). Gentamycin was effective drug as all isolates (n = 43; 100%) were susceptible to it and followed by kanamycin (n = 39; 90.7%). However, the majority of the isolates showed resistance to penicillin-G (95.3%), nalidixic acid (88.4%), cloxacillin (79.1%), vancomycin (65.1%) and cefoxitin (55.8%). Of the 15 S. aureus tested for drug susceptibility, 73.3% of them were phenotypically resistant to vancomycin (VRSA) and all of the 15 isolates showed multi-drug resistance (MDR) to >3 drugs. Also, all of the tested CNS (100%), S. hyicus (100%) and the majority of S. intermedius isolates (88.9%) developed MDR. CONCLUSION: Alarmingly, the Staphylococcus isolates circulating in the dairy farms and abattoir in the study area harbor MDR. High level of Staphylococcus species isolation from personnel and equipment besides food (meat and milk) samples in dairy farms and abattoir settings reveals that the hygiene practice in the dairy farm and abattoir is substandard. Prudent drug use and improved hygienic practice is recommended in the dairy farms and abattoir to safeguard the public from the risk of acquiring infections and MDR pathogenic Staphylococcus.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana/genética
Mastite Bovina/epidemiologia
Staphylococcus aureus Resistente à Meticilina/genética
Saúde do Trabalhador/educação
Infecções Estafilocócicas/epidemiologia
Staphylococcus/genética
[Mh] Termos MeSH secundário: Matadouros
Criação de Animais Domésticos
Animais
Antibacterianos/farmacologia
Técnicas de Tipagem Bacteriana
Bovinos
Estudos Transversais
Etiópia/epidemiologia
Fazendas
Feminino
Gentamicinas/farmacologia
Seres Humanos
Canamicina/farmacologia
Mastite Bovina/microbiologia
Mastite Bovina/transmissão
Carne/microbiologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Leite/microbiologia
Prevalência
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/transmissão
Staphylococcus/classificação
Staphylococcus/efeitos dos fármacos
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-017-2487-y


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[PMID]:27776591
[Au] Autor:Lopez AL; Aldaba JG; Ama CG; Sylim PG; Geraldino XD; Sarol JN; Salonga AM
[Ad] Endereço:National Institutes of Health, University of the Philippines Manila, Manila, The Philippines.
[Ti] Título:Surveillance for tuberculosis in a rural community in The Philippines.
[So] Source:Int J Tuberc Lung Dis;20(11):1495-1500, 2016 Nov.
[Is] ISSN:1815-7920
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:SETTING: Estimates of the tuberculosis (TB) burden in the Philippines are largely dependent on prevalence surveys. OBJECTIVE: To conduct a prospective community-based survey to generate epidemiological data on TB among patients seeking care in public health centres in a rural municipality in the Philippines. DESIGN: Prospective surveillance and follow-up of presumptive TB cases from May 2013 to July 2015. RESULTS: Of 1622 participants with presumptive TB, 468 (28.8%) (95%CI 26.6-31.1) were diagnosed with TB. The annual TB case notification rate in San Juan was 212 (95%CI 184-242) per 100 000 population. There were nine TB-attributable deaths during the study period. Only 8.8% (95%CI 6.2-11.32) of the cases were children aged <15 years; 274 (58.5%) cases were bacteriologically confirmed. Of 210 isolates tested for antimicrobial resistance, 49 (23.3%, 95%CI 17.58-29.02) were resistant. Resistance to isoniazid (INH) was common (n = 33, 15.7%); multidrug-resistant TB was 1.9%. CONCLUSION: TB remains an important health problem in the Philippines. We identified low case detection of TB in children and high INH resistance rates in this rural community.
[Mh] Termos MeSH primário: Vigilância da População
População Rural
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
Tuberculose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Antituberculosos/uso terapêutico
Criança
Pré-Escolar
Feminino
Seguimentos
Seres Humanos
Lactente
Recém-Nascido
Isoniazida/uso terapêutico
Canamicina/uso terapêutico
Masculino
Meia-Idade
Filipinas/epidemiologia
Prevalência
Estudos Prospectivos
Saúde Pública
Rifampina/uso terapêutico
Estreptomicina/uso terapêutico
Resultado do Tratamento
Tuberculose/diagnóstico
Tuberculose/tratamento farmacológico
Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico
Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 59-01-8 (Kanamycin); V83O1VOZ8L (Isoniazid); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28753625
[Au] Autor:Gao W; Wu Z; Sun J; Ni X; Xia H
[Ad] Endereço:School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, No.103 Wenhua Road, Shenyang, Liaoning, China.
[Ti] Título:Modulation of kanamycin B and kanamycin A biosynthesis in Streptomyces kanamyceticus via metabolic engineering.
[So] Source:PLoS One;12(7):e0181971, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Both kanamycin A and kanamycin B, antibiotic components produced by Streptomyces kanamyceticus, have medical value. Two different pathways for kanamycin biosynthesis have been reported by two research groups. In this study, to obtain an optimal kanamycin A-producing strain and a kanamycin B-high-yield strain, we first examined the native kanamycin biosynthetic pathway in vivo. Based on the proposed parallel biosynthetic pathway, kanN disruption should lead to kanamycin A accumulation; however, the kanN-disruption strain produced neither kanamycin A nor kanamycin B. We then tested the function of kanJ and kanK. The main metabolite of the kanJ-disruption strain was identified as kanamycin B. These results clarified that kanamycin biosynthesis does not proceed through the parallel pathway and that synthesis of kanamycin A from kanamycin B is catalyzed by KanJ and KanK in S. kanamyceticus. As expected, the kanamycin B yield of the kanJ-disruption strain was 3268±255 µg/mL, 12-fold higher than that of the original strain. To improve the purity of kanamycin A and reduce the yield of kanamycin B in the fermentation broth, four different kanJ- and kanK-overexpressing strains were constructed through either homologous recombination or site-specific integration. The overexpressing strain containing three copies of kanJ and kanK in its genome exhibited the lowest kanamycin B yield (128±20 µg/mL), which was 54% lower than that of the original strain. Our experimental results demonstrate that kanamycin A is derived from KanJ-and-KanK-catalyzed conversion of kanamycin B in S. kanamyceticus. Moreover, based on the clarified biosynthetic pathway, we obtained a kanamycin B-high-yield strain and an optimized kanamycin A-producing strain with minimal byproduct.
[Mh] Termos MeSH primário: Vias Biossintéticas
Canamicina/análogos & derivados
Canamicina/biossíntese
Engenharia Metabólica/métodos
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Vias Biossintéticas/genética
Proliferação Celular
Genótipo
Metaboloma
Metabolômica
Família Multigênica
Reação em Cadeia da Polimerase em Tempo Real
Recombinação Genética/genética
Streptomyces/genética
Streptomyces/crescimento & desenvolvimento
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
15JT14C3GI (bekanamycin); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181971


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[PMID]:28654744
[Au] Autor:Luan Q; Gan N; Cao Y; Li T
[Ad] Endereço:State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University , Ningbo, 315211, PR China.
[Ti] Título:Mimicking an Enzyme-Based Colorimetric Aptasensor for Antibiotic Residue Detection in Milk Combining Magnetic Loop-DNA Probes and CHA-Assisted Target Recycling Amplification.
[So] Source:J Agric Food Chem;65(28):5731-5740, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A mimicking-enzyme-based colorimetric aptasensor was developed for the detection of kanamycin (KANA) in milk using magnetic loop-DNA-NMOF-Pt (m-L-DNA) probes and catalytic hairpin assembly (CHA)-assisted target recycling for signal amplification. The m-L-DNA probes were constructed via hybridization of hairpin DNA H1 (containing aptamer sequence) immobilized magnetic beads (m-H1) and signal DNA (sDNA, partial hybridization with H1) labeled nano Fe-MIL-88NH -Pt (NMOF-Pt-sDNA). In the presence of KANA and complementary hairpin DNA H2, the m-L-DNA probes decomposed and formed an m-H1/KANA intermediate, which triggered the CHA reaction to form a stable duplex strand (m-H1-H2) while releasing KANA again for recycling. Consequently, numerous NMOF-Pt-sDNA as mimicking enzymes can synergistically catalyze 3,3',5,5'-tetramethylbenzidine (TMB) for color development. The aptasensor exhibited high selectivity and sensitivity for KANA in milk with a detection limit of 0.2 pg mL within 30 min. The assay can be conveniently extended for on-site screening of other antibiotics in foods by simply changing the base sequence of the probes.
[Mh] Termos MeSH primário: Antibacterianos/análise
Técnicas Biossensoriais/métodos
Resíduos de Drogas/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Aptâmeros de Nucleotídeos/química
Benzidinas/química
Técnicas Biossensoriais/instrumentação
Bovinos
Colorimetria
DNA/química
Contaminação de Alimentos/análise
Ouro/química
Canamicina/análise
Limite de Detecção
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Aptamers, Nucleotide); 0 (Benzidines); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 59-01-8 (Kanamycin); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02139


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[PMID]:28640905
[Au] Autor:Zhao T; Wang Z; Su L; Sun X; Cheng J; Zhang L; Karungo SK; Han Y; Li S; Xin H
[Ad] Endereço:Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P.R. China.
[Ti] Título:An efficient method for transgenic callus induction from Vitis amurensis petiole.
[So] Source:PLoS One;12(6):e0179730, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species. Herein, an efficient method was established to produce transformed calli of V. amurensis. Segments of petioles from micropropagated plantlets of V. amurensis exhibited better capacity to differentiate calli than leaf-discs and stem segments, and thus was chosen as target tissue for Agrobacterium-mediated transformation. Both neomycin phosphotransferase II (NPTII) and enhanced green fluorescent protein (eGFP) genes were used for simultaneous selection of transgenic calli based on kanamycin resistance and eGFP fluorescence. Several parameters affecting the transformation efficiency were optimized including the concentration of kanamycin, Agrobacterium stains, bacterial densities, infection treatments and co-cultivation time. The transgenic callus lines were verified by checking the integration of NPTII gene into calli genomes, the expression of eGFP gene and the fluorescence of eGFP. Up to 20% of the petiole segments produced transformed calli after 2 months of cultivation. This efficient transformation system will facilitate the functional analysis of agronomic characteristics and related genes not only in V. amurensis but also in other grapevine species.
[Mh] Termos MeSH primário: Engenharia Genética/métodos
Folhas de Planta/genética
Transformação Genética
Vitis/genética
[Mh] Termos MeSH secundário: Agrobacterium/genética
Agrobacterium/crescimento & desenvolvimento
Técnicas de Cocultura
Relação Dose-Resposta a Droga
Proteínas de Fluorescência Verde/genética
Canamicina/farmacologia
Plantas Geneticamente Modificadas
Fatores de Tempo
Vitis/efeitos dos fármacos
Vitis/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179730


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[PMID]:28612062
[Au] Autor:Olajuyigbe OO; Adeoye-Isijola MO; Okon V; Adedayo O; Coopoosamy RM
[Ad] Endereço:Biosciences & Biotechnology Department, Babcock University, PMB 4005, Ilisan Remo, Ogun State, Nigeria.
[Ti] Título:In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens.
[So] Source:Acta Biochim Pol;64(2):255-263, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The in vitro antibacterial activity of pure caffeine powder and its interaction with first line antibiotic against bacterial isolates were investigated with the macrobroth dilution and the checkerboard assay methods. This study showed that caffeine and the antibiotics exhibited various degrees of antibacterial activities. While caffeine had MICs ranging between 67.19 and 268.75 µg/ml, chloramphenicol was characterized by MICs between 0.98 and 31.25 µg/ml, kanamycin - 15.63-62.5 µg/ml, nalidixic acid - 0.49-250 µg/ml, erythromycin - 0.49-62.5 µg/ml, tetracycline - 1.99-62.5 µg/ml and metronidazole - 15.63-31.25 µg/ml. Combining ½ MICs and MICs of caffeine with the antibiotics as well as direct combination of caffeine and the antibiotics resulted in significant reduction of antibiotics' effectiveness. The fractional inhibitory concentration index (FICI) for the combination of ½ MICs of caffeine with different antibiotics showed antagonistic interactions with the antibiotics except kanamycin which had additive and indifferent interactions with caffeine. The FICI of the MICs of caffeine combined with antibiotics showed a reduction in the number of antagonistic interactions as chloramphenicol, nalidixic acid and erythromycin showed some indifferent interactions while kanamycin was the only antibiotic that showed indifferent interaction against all the bacterial isolates. The direct combination of caffeine and the antibiotics resulted in significant antagonistic interactions higher than in the case when caffeine, at the ½ MICs and MICs, was combined with the antibiotics. Although caffeine demonstrated significant antibacterial activity against the selected bacterial isolates, its combination with the selected antibiotics resulted in significant antagonistic interactions. Caffeine should not be combined with antibiotics as this could result in serious therapeutic failure and, possibly, drug toxicity in vivo.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Cafeína/farmacologia
Sinergismo Farmacológico
[Mh] Termos MeSH secundário: Bactérias/patogenicidade
Cafeína/química
Cloranfenicol/farmacologia
Eritromicina/farmacologia
Seres Humanos
Canamicina/farmacologia
Metronidazol/farmacologia
Testes de Sensibilidade Microbiana
Ácido Nalidíxico/farmacologia
Tetraciclina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 140QMO216E (Metronidazole); 3B91HWA56M (Nalidixic Acid); 3G6A5W338E (Caffeine); 59-01-8 (Kanamycin); 63937KV33D (Erythromycin); 66974FR9Q1 (Chloramphenicol); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2016_1327


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[PMID]:28500800
[Au] Autor:Varghese B; Al-Hajoj S
[Ad] Endereço:Mycobacteriology Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
[Ti] Título:First Insight Into the Fluoroquinolone and Aminoglycoside Resistance of Multidrug-Resistant in Saudi Arabia.
[So] Source:Am J Trop Med Hyg;96(5):1066-1070, 2017 May.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AbstractIn Saudi Arabia, there were no nationwide screening studies conducted so far to determine the aminoglycoside and fluoroquinolone resistance among multidrug-resistant tuberculosis (MDR-TB) isolates. Therefore, as the first attempt in the country, a retrospective analysis has been conducted on a nationwide collection of 2,956 clinical isolates screened with phenotypic drug susceptibility testing to define MDR-TB. Enrolled MDR-TB isolates were subjected to second-line drug susceptibility testing, detection of mutations conferring resistance to aminoglycosides and fluoroquinolone, followed by 24-loci mycobacterial interspersed repetitive unit-variable number of tandem repeat typing and spoligotyping. Overall, 83 isolates were identified as MDR-TB, and 13 (15.7%) isolates showed resistance to second-line drugs. Moxifloxacin (low level) showed higher resistant rates (10.8%) followed by ofloxacin (7.2%), capreomycin (3.6%), kanamycin (3.6%), and amikacin (2.4%). Overall fluoroquinolone resistance was 12%, whereas aminoglycoside resistance was 7.2%. Predominant mutations conferring resistance to fluoroquinolone were found in A90V and D94G, whereas aminoglycoside resistance was observed only with gene A1401G mutation. The corresponding strain lineages predominated with Indo-Oceanic and East-African Indian origin. Interestingly, none of the isolates with second-line drug resistance was defined as extensively drug-resistant TB (XDR-TB). Surprisingly, many isolates (50.6%) were panresistant to first-line drugs. Saudi Arabia faces considerable burden of fluoroquinolone- and aminoglycoside-resistant MDR-TB. Higher incidence of panresistant MDR-TB reveals a threat for the emergence of XDR-TB strains in the near future.
[Mh] Termos MeSH primário: Antituberculosos/uso terapêutico
Farmacorresistência Bacteriana Múltipla/genética
Tuberculose Extensivamente Resistente a Medicamentos/tratamento farmacológico
Genes Bacterianos
Mycobacterium tuberculosis/isolamento & purificação
Tuberculose Pulmonar/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Idoso
Amicacina/uso terapêutico
Capreomicina/uso terapêutico
Tuberculose Extensivamente Resistente a Medicamentos/diagnóstico
Tuberculose Extensivamente Resistente a Medicamentos/microbiologia
Feminino
Fluoroquinolonas/uso terapêutico
Seres Humanos
Canamicina/uso terapêutico
Masculino
Testes de Sensibilidade Microbiana
Tipagem Molecular
Mutação
Mycobacterium tuberculosis/classificação
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/genética
Estudos Retrospectivos
Fatores de Risco
Arábia Saudita
Sequências de Repetição em Tandem
Tuberculose Pulmonar/diagnóstico
Tuberculose Pulmonar/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Fluoroquinolones); 11003-38-6 (Capreomycin); 59-01-8 (Kanamycin); 84319SGC3C (Amikacin); U188XYD42P (moxifloxacin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170514
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0579


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[PMID]:28475419
[Au] Autor:Kaneshima T; Myoda T; Toeda K; Fujimori T; Nishizawa M
[Ad] Endereço:a Department of Food and Cosmetic Science , Tokyo University of Agriculture , Abashiri , Japan.
[Ti] Título:Antimicrobial constituents of peel and seeds of camu-camu (Myrciaria dubia).
[So] Source:Biosci Biotechnol Biochem;81(8):1461-1465, 2017 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Various antimicrobial constituents of camu-camu fruit were isolated. Acylphloroglucinol (compound 1) and rhodomyrtone (compound 2) were isolated from the peel of camu-camu (Myrciaria dubia) fruit, while two other acylphloroglucinols (compounds 3 and 4) were obtained from camu-camu seeds. The structures of the isolated compounds were characterized by spectrophotometric methods. Compounds 1 and 4 were confirmed to be new acylphloroglucinols with different substituents at the C7 or C9 position of 2, and were named myrciarone A and B, respectively. Compound 3 was determined to be isomyrtucommulone B. This is the first report of the isolation of 3 from a natural resource. The antimicrobial activities of compounds 1, 3, and 4 were similar to those of 2, and the minimum inhibitory concentrations were either similar to or lower than that of kanamycin. These results suggest that the peel and seeds of camu-camu fruit could be utilized for therapeutic applications.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Frutas/química
Myrtaceae/química
Floroglucinol/isolamento & purificação
Sementes/química
Xantonas/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Candida albicans/efeitos dos fármacos
Candida albicans/crescimento & desenvolvimento
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Canamicina/farmacologia
Testes de Sensibilidade Microbiana
Estrutura Molecular
Floroglucinol/análogos & derivados
Floroglucinol/farmacologia
Extratos Vegetais/química
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/crescimento & desenvolvimento
Xantonas/isolamento & purificação
Xantonas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Plant Extracts); 0 (Xanthones); 0 (rhodomyrtone); 59-01-8 (Kanamycin); DHD7FFG6YS (Phloroglucinol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1320517


  9 / 5694 MEDLINE  
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[PMID]:28359737
[Au] Autor:Kraemer LS; Brenner TA; Krumholz JO; Rosenberg HF
[Ad] Endereço:Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
[Ti] Título:A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.
[So] Source:J Microbiol Methods;137:19-24, 2017 Jun.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.
[Mh] Termos MeSH primário: Eosinófilos/microbiologia
Citometria de Fluxo/métodos
Lactobacillus reuteri/metabolismo
Probióticos/farmacocinética
[Mh] Termos MeSH secundário: Aminoglicosídeos/química
Animais
Escherichia coli/efeitos dos fármacos
Granulócitos/microbiologia
Mucosa Intestinal/citologia
Mucosa Intestinal/microbiologia
Canamicina/farmacologia
Lactobacillus reuteri/isolamento & purificação
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


  10 / 5694 MEDLINE  
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[PMID]:28343755
[Au] Autor:Shavit M; Pokrovskaya V; Belakhov V; Baasov T
[Ad] Endereço:The Edith and Joseph Fischer Enzyme Inhibitors Laboratory, Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa 3200003, Israel.
[Ti] Título:Covalently linked kanamycin - Ciprofloxacin hybrid antibiotics as a tool to fight bacterial resistance.
[So] Source:Bioorg Med Chem;25(11):2917-2925, 2017 Jun 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To address the growing problem of antibiotic resistance, a set of 12 hybrid compounds that covalently link fluoroquinolone (ciprofloxacin) and aminoglycoside (kanamycin A) antibiotics were synthesized, and their activity was determined against both Gram-negative and Gram-positive bacteria, including resistant strains. The hybrids were antagonistic relative to the ciprofloxacin, but were substantially more potent than the parent kanamycin against Gram-negative bacteria, and overcame most dominant resistance mechanisms to aminoglycosides. Selected hybrids were 42-640 fold poorer inhibitors of bacterial protein synthesis than the parent kanamycin, while they displayed similar inhibitory activity to that of ciprofloxacin against DNA gyrase and topoisomerase IV enzymes. The hybrids showed significant delay of resistance development in both E. coli and B. subtilis in comparison to that of component drugs alone or their 1:1 mixture. More generally, the data suggest that an antagonistic combination of aminoglycoside-fluoroquinolone hybrids can lead to new compounds that slowdown/prevent the emergence of resistance.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Ciprofloxacino/farmacologia
Farmacorresistência Bacteriana/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Canamicina/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Antibacterianos/química
Ciprofloxacino/química
Relação Dose-Resposta a Droga
Canamicina/química
Testes de Sensibilidade Microbiana
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 59-01-8 (Kanamycin); 5E8K9I0O4U (Ciprofloxacin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE



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