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[PMID]:29214789
[Au] Autor:Kim CW; Han JH; Wu L; Choi JY
[Ad] Endereço:Department of Otorhinolaryngology, Hallym University College of Medicine, Seoul, Korea.
[Ti] Título:microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish.
[So] Source:Yonsei Med J;59(1):141-147, 2018 Jan.
[Is] ISSN:1976-2437
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: microRNAs (miRNAs) are non-coding RNAs composed of 20 to 22 nucleotides that regulate development and differentiation in various organs by silencing specific RNAs and regulating gene expression. In the present study, we show that the microRNA (miR)-183 cluster is upregulated during hair cell regeneration and that its inhibition reduces hair cell regeneration following neomycin-induced ototoxicity in zebrafish. MATERIALS AND METHODS: miRNA expression patterns after neomycin exposure were analyzed using microarray chips. Quantitative polymerase chain reaction was performed to validate miR-183 cluster expression patterns following neomycin exposure (500 µM for 2 h). After injection of an antisense morpholino (MO) to miR-183 (MO-183) immediately after fertilization, hair cell regeneration after neomycin exposure in neuromast cells was evaluated by fluorescent staining (YO-PRO1). The MO-183 effect also was assessed in transgenic zebrafish larvae expressing green fluorescent protein (GFP) in inner ear hair cells. RESULTS: Microarray analysis clearly showed that the miR-183 cluster (miR-96, miR-182, and miR-183) was upregulated after neomycin treatment. We also confirmed upregulated expression of the miR-183 cluster during hair cell regeneration after neomycin-induced ototoxicity. miR-183 inhibition using MO-183 reduced hair cell regeneration in both wild-type and GFP transgenic zebrafish larvae. CONCLUSION: Our work demonstrates that the miR-183 cluster is essential for the regeneration of hair cells following ototoxic injury in zebrafish larvae. Therefore, regulation of the miR-183 cluster can be a novel target for stimulation of hair cell regeneration.
[Mh] Termos MeSH primário: Células Ciliadas Auditivas/fisiologia
MicroRNAs/metabolismo
Regeneração/genética
Peixe-Zebra/genética
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Contagem de Células
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/efeitos dos fármacos
Técnicas de Silenciamento de Genes
Proteínas de Fluorescência Verde/metabolismo
Células Ciliadas Auditivas/efeitos dos fármacos
Larva/efeitos dos fármacos
Larva/genética
MicroRNAs/genética
Morfolinos/farmacologia
Neomicina/toxicidade
Regeneração/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN183 microRNA, zebrafish); 0 (MicroRNAs); 0 (Morpholinos); 1404-04-2 (Neomycin); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.3349/ymj.2018.59.1.141


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[PMID]:29292089
[Au] Autor:Yu X; Fan Z; Han Y; Zhang D; Xu L; Wang M; Yang Q; Li H; Zhou M; Zhang L; Sun G; Bai X; Li J; Wang H
[Ad] Endereço:Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, China; Shandong Institute of Otolaryngology, Jinan, China.
[Ti] Título:Paeoniflorin reduces neomycin-induced ototoxicity in hair cells by suppression of reactive oxygen species generation and extracellularly regulated kinase signalization.
[So] Source:Toxicol Lett;285:9-19, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study was designed to investigate the effect of paeoniflorin (PF) on neomycin-induced ototoxicity in hair cells (HCs). Here, we took advantage of C57BL/6 mice and cochlear explants culture to determine the role of PF in vivo and in vitro. We demonstrated that neomycin exposure induced severe hearing loss and HC damage, which was mediated by activated mitochondrial apoptosis pathway, promoted extracellular signal-regulated kinase (ERK) signaling as well as enhanced reactive oxygen species (ROS) generation in HCs. Interestingly, we found that PF pretreatment significantly alleviated neomycin-induced hearing loss, attenuated HC injury and decreased HC apoptosis caused by neomycin. Mechanistic studies revealed that PF could decrease cellular ROS levels, suppress the activation of ERK signaling and, subsequently, mitigate the imbalance of mitochondrial apoptotic pathway, thus protecting HCs from neomycin-induced apoptosis. This study indicates that PF may serve as an antioxidative and anti-apoptotic agent to prevent hearing loss caused by neomycin.
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Glucosídeos/uso terapêutico
Células Ciliadas Auditivas/efeitos dos fármacos
Perda Auditiva/prevenção & controle
Monoterpenos/uso terapêutico
Neomicina/toxicidade
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes/administração & dosagem
Apoptose/efeitos dos fármacos
Células Cultivadas
Potenciais Evocados Auditivos do Tronco Encefálico/efeitos dos fármacos
Glucosídeos/administração & dosagem
Células Ciliadas Auditivas/metabolismo
Células Ciliadas Auditivas/patologia
Perda Auditiva/induzido quimicamente
Perda Auditiva/metabolismo
Perda Auditiva/patologia
Camundongos Endogâmicos C57BL
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/patologia
Monoterpenos/administração & dosagem
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Glucosides); 0 (Monoterpenes); 0 (Reactive Oxygen Species); 1404-04-2 (Neomycin); 21AIQ4EV64 (peoniflorin); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


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[PMID]:27773647
[Au] Autor:Kandathil CK; Stakhovskaya O; Leake PA
[Ad] Endereço:Epstein Laboratory, Dept. of Otolaryngology-Head & Neck Surgery, University of California San Francisco, 533 Parnassus Avenue, Room U490, San Francisco, CA 94143-0526, USA. Electronic address: cherian.kandathil@outlook.com.
[Ti] Título:Effects of brain-derived neurotrophic factor (BDNF) on the cochlear nucleus in cats deafened as neonates.
[So] Source:Hear Res;342:134-143, 2016 Dec.
[Is] ISSN:1878-5891
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Many previous studies have shown significant neurotrophic effects of intracochlear delivery of BDNF in preventing degeneration of cochlear spiral ganglion (SG) neurons after deafness in rodents and our laboratory has shown similar results in developing cats deafened prior to hearing onset. This study examined the morphology of the cochlear nucleus (CN) in a group of neonatally deafened cats from a previous study in which infusion of BDNF elicited a significant improvement in survival of the SG neurons. Five cats were deafened by systemic injections of neomycin sulfate (60 mg/kg, SQ, SID) starting one day after birth, and continuing for 16-18 days until auditory brainstem response (ABR) testing demonstrated profound bilateral hearing loss. The animals were implanted unilaterally at about 1 month of age using custom-designed electrodes with a drug-delivery cannula connected to an osmotic pump. BDNF (94 µg/ml; 0.25 µl/hr) was delivered for 10 weeks. The animals were euthanized and studied at 14-23 weeks of age. Consistent with the neurotrophic effects of BDNF on SG survival, the total CN volume in these animals was significantly larger on the BDNF-treated side than on the contralateral side. However, total CN volume, both ipsi- and contralateral to the implants in these deafened juvenile animals, was markedly smaller than the CN in normal adult animals, reflecting the severe effects of deafness on the central auditory system during development. Data from the individual major CN subdivisions (DCN, Dorsal Cochlear Nucleus; PVCN, Posteroventral Cochlear Nucleus; AVCN, Anteroventral Cochlear Nucleus) also were analyzed. A significant difference was observed between the BDNF-treated and control sides only in the AVCN. Measurements of the cross-sectional areas of spherical cells showed that cells were significantly larger in the AVCN ipsilateral to the implant than on the contralateral side. Further, the numerical density of spherical cells was significantly lower in the AVCN ipsilateral to the implant than on the contralateral side, consistent with the larger AVCN volume observed with BDNF treatment. Together, findings indicate significant neurotrophic effects of intracochlear BDNF infusion on the developing CN.
[Mh] Termos MeSH primário: Fator Neurotrófico Derivado do Encéfalo/administração & dosagem
Núcleo Coclear/efeitos dos fármacos
Núcleo Coclear/patologia
Surdez/tratamento farmacológico
Surdez/patologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Gatos
Núcleo Coclear/crescimento & desenvolvimento
Surdez/induzido quimicamente
Sistemas de Liberação de Medicamentos
Potenciais Evocados Auditivos do Tronco Encefálico
Neomicina/toxicidade
Tamanho do Órgão/efeitos dos fármacos
Gânglio Espiral da Cóclea/efeitos dos fármacos
Gânglio Espiral da Cóclea/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brain-Derived Neurotrophic Factor); 1404-04-2 (Neomycin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:28438376
[Au] Autor:Wheat CM; Bickley RJ; Hsueh YH; Cohen BA
[Ad] Endereço:Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD. Electronic address: cwheat1@jhmi.edu.
[Ti] Título:Current Trends in the Use of Two Combination Antifungal/Corticosteroid Creams.
[So] Source:J Pediatr;186:192-195.e1, 2017 Jul.
[Is] ISSN:1097-6833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Superficial fungal infections are among the most commonly managed skin problems by general practitioners. Although evidence shows combination antifungal/corticosteroid topicals are more expensive and less effective than single-agent antifungals, practitioners continue to prescribe combination agents. We examined current prescription trends of 2 combination antifungal/corticosteroid medications, Lotrisone and Mycolog-II.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Antifúngicos/uso terapêutico
Betametasona/uso terapêutico
Clotrimazol/uso terapêutico
Dermatomicoses/tratamento farmacológico
Glucocorticoides/uso terapêutico
Gramicidina/uso terapêutico
Neomicina/uso terapêutico
Nistatina/uso terapêutico
Triancinolona Acetonida/uso terapêutico
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Combinação de Medicamentos
Seres Humanos
Lactente
Recém-Nascido
Padrões de Prática Médica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antifungal Agents); 0 (Drug Combinations); 0 (Glucocorticoids); 0 (Gramicidin, Neomycin Sulfate, Nystatin, Triamcinolone Acetonide Drug Combination); 0 (Lotrisone); 1400-61-9 (Nystatin); 1404-04-2 (Neomycin); 1405-97-6 (Gramicidin); 9842X06Q6M (Betamethasone); F446C597KA (Triamcinolone Acetonide); G07GZ97H65 (Clotrimazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


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[PMID]:28373074
[Au] Autor:Yamahara K; Nakagawa T; Ito J; Kinoshita K; Omori K; Yamamoto N
[Ad] Endereço:Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Kyoto 606-8507, Japan.
[Ti] Título:Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells.
[So] Source:Neuropharmacology;119:26-39, 2017 Jun.
[Is] ISSN:1873-7064
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sensorineural hearing loss (SNHL) is mainly caused by the damage of cochlear hair cells (HCs). As HCs and supporting cells (SCs) do not proliferate in postnatal mammals, the loss of HCs and SCs is irreversible, emphasizing the importance of preserving their numbers to prevent SNHL. It is known that insulin-like growth factor 1 (IGF1) is instrumental in the treatment of SNHL. Our previous study indicates that IGF1 protects HCs against aminoglycoside by activating IGF1 receptor and its two major downstream pathways, PI3K/AKT and MEK/ERK, in SCs, which results in the upregulation of the expression of the Netrin1-encoding gene (Ntn1). However, the mechanisms underlying IGF1-induced protection of HCs via SC activation as well as the role of NTN1 in this process have not been elucidated. Here, we demonstrated that NTN1, similar to IGF1, promoted HC survival. NTN1 blocking antibody attenuated IGF1-induced HC protection from aminoglycoside, indicating that NTN1 is the effector molecule of IGF1 signaling during HC protection. In situ hybridization demonstrated that IGF1 potently induced Ntn1 expression in SCs. NTN1 receptors were abundantly expressed in the cochlea; among them, UNC5B mediated IGF1 protective effects on HCs, as NTN1 binding to UNC5B inhibited HC apoptosis. These results provide new insights into the mechanisms underlying IGF1 protection of cochlear HCs, suggesting a possibility of using NTN1 as a new treatment for SNHL.
[Mh] Termos MeSH primário: Cóclea/citologia
Células Ciliadas Auditivas/efeitos dos fármacos
Fator de Crescimento Insulin-Like I/metabolismo
Fatores de Crescimento Neural/metabolismo
Fatores de Crescimento Neural/farmacologia
Proteínas Supressoras de Tumor/metabolismo
Proteínas Supressoras de Tumor/farmacologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Antibacterianos/farmacologia
Anticorpos/farmacologia
Caspase 3/metabolismo
Contagem de Células
Inibidores Enzimáticos/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Técnicas In Vitro
Camundongos
Camundongos Endogâmicos ICR
Neomicina/farmacologia
Fatores de Crescimento Neural/imunologia
Receptores de Netrina
Netrina-1
Técnicas de Cultura de Órgãos
Fosfatidilinositol 3-Quinases/metabolismo
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Transdução de Sinais/efeitos dos fármacos
Proteínas Supressoras de Tumor/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antibodies); 0 (Enzyme Inhibitors); 0 (Nerve Growth Factors); 0 (Netrin Receptors); 0 (Ntn1 protein, mouse); 0 (Receptors, Cell Surface); 0 (Tumor Suppressor Proteins); 1404-04-2 (Neomycin); 158651-98-0 (Netrin-1); 67763-96-6 (Insulin-Like Growth Factor I); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28250994
[Au] Autor:Wang C; Zhong Z; Sun P; Zhong H; Li H; Chen F
[Ad] Endereço:Department of Biomedical Engineering, South University of Science and Technology of China, Guangdong, China.
[Ti] Título:Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses.
[So] Source:Neural Plast;2017:8283075, 2017.
[Is] ISSN:1687-5443
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their function. Startle response is a behavior closely related to the auditory function of hair cells; therefore it can be used to measure the function of hair cells. In this study, we developed a device to measure the startle response of zebrafish larvae. By applying various levels of stimulus, it showed that the system can discern a 10 dB difference. The hair cell in zebrafish can regenerate after damage due to noise exposure or drug treatment. With this device, we measured the startle response of zebrafish larvae during and after drug treatment. The results show a similar trend to the classical hair cell staining method. The startle response was reduced with drug treatment and recovered after removal of the drug. Together it demonstrated the capability of this behavioral assay in evaluating the hair cell functions of fish larvae and its potential as a high-throughput screening tool for auditory-related gene and drug discovery.
[Mh] Termos MeSH primário: Comportamento Animal
Células Ciliadas Auditivas Internas/fisiologia
Reflexo de Sobressalto
Regeneração
[Mh] Termos MeSH secundário: Estimulação Acústica/métodos
Animais
Células Ciliadas Auditivas Internas/efeitos dos fármacos
Células Ciliadas Auditivas Internas/patologia
Larva
Neomicina/administração & dosagem
Reflexo de Sobressalto/efeitos dos fármacos
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1404-04-2 (Neomycin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1155/2017/8283075


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[PMID]:28129992
[Au] Autor:Watkins D; Gong C; Kellish P; Arya DP
[Ad] Endereço:NUBAD, LLC, Greenville, SC 29607, USA.
[Ti] Título:Probing A-form DNA: A fluorescent aminosugar probe and dual recognition by anthraquinone-neomycin conjugates.
[So] Source:Bioorg Med Chem;25(4):1309-1319, 2017 Feb 15.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nucleic acids adopt a broad array of hydrogen-bonded structures that enable their diverse roles in the cell; even the familiar DNA double helix displays subtle architectural nuances that are sequence dependent. While there have been many approaches for recognition of B-form nucleic acids, A-form DNA recognition has lagged behind. Here, using a tight binding fluorescein-neomycin (F-neo) conjugate that can probe the electrostatic environment of A-form DNA major groove, we developed a fluorescent displacement assay to be used as a screen for DNA duplex-binding compounds. As opposed to intercalating dyes that can significantly perturb DNA structure, the groove binding F-neo allows the probing of native DNA conformation. In combination with the assay development and probing of DNA grooves, we also report the synthesis and binding of a series of neomycin-anthraquinone conjugates, two units with a known preference for binding GC rich DNA. The assay can be used to identify duplex DNA-binding compounds, as well as probe structural features of a target DNA duplex, and can easily be scaled up for high throughput screening of compound libraries.
[Mh] Termos MeSH primário: DNA Forma A/análise
Fluoresceína/química
Corantes Fluorescentes/análise
Neomicina/química
[Mh] Termos MeSH secundário: Simulação de Acoplamento Molecular
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, A-Form); 0 (Fluorescent Dyes); 1404-04-2 (Neomycin); TPY09G7XIR (Fluorescein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


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[PMID]:28093206
[Au] Autor:Wang J; Zhao Q; Jiang N; Li W; Chen L; Lin X; Xie Z; You L; Zhang Q
[Ad] Endereço:Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China. Electronic address: jbwang@fzu.edu.cn.
[Ti] Título:Urea-formaldehyde monolithic column for hydrophilic in-tube solid-phase microextraction of aminoglycosides.
[So] Source:J Chromatogr A;1485:24-31, 2017 Feb 17.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel urea-formaldehyde (UF) monolithic column has been developed and exploited as a sorbent for hydrophilic in-tube solid-phase microextraction (in-tube SPME) of aminoglycosides (AGs). Because of the innate hydrophilicity, UF monolith showed high extraction efficiency towards these hydrophilic analytes. The adsorption capacities for target compounds dissolved in water/ACN (1:1, v/v) were in the range of 5.18-7.36µg/cm. Due to the lack of a chromophore, evaporative light scattering detector (ELSD) was selected as the detector for AGs, and coupled with the online in-tube SPME-HPLC system. Several factors of the online system, such as trifluoroacetic acid (TFA) and ACN percentage in the sampling solution, ionic strength in the sample solution, elution volume, sampling and elution flow rate, were optimized with respect to the extraction efficiencies. Under the optimized conditions, the limits of detection (LODs) of streptomycin, tobramycin and neomycin were discovered in the range of 3.0-5.2µg/kg. The recoveries were ranged from 82.1 to 96.7% with relative standard deviations (RSDs) of 2.3-5.1% (n=4) at spiking levels of 50, 200 and 500µg/kg, respectively. The excellent applicability of the UF monolithic column was examined by the determination of streptomycin in practical tilapia samples, which showed the potential advantages for the analysis of polar analytes in complicated samples.
[Mh] Termos MeSH primário: Aminoglicosídeos/isolamento & purificação
Antibacterianos/isolamento & purificação
Microextração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Adsorção
Animais
Cromatografia Líquida de Alta Pressão
Interações Hidrofóbicas e Hidrofílicas
Limite de Detecção
Neomicina/isolamento & purificação
Polímeros/síntese química
Microextração em Fase Sólida/instrumentação
Estreptomicina/isolamento & purificação
Tilápia
Tobramicina/isolamento & purificação
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Anti-Bacterial Agents); 0 (Polymers); 059QF0KO0R (Water); 1404-04-2 (Neomycin); VZ8RRZ51VK (Tobramycin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE


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[PMID]:28064050
[Au] Autor:Jabeen S; Islam A; Ghaffar A; Gull N; Hameed A; Bashir A; Jamil T; Hussain T
[Ad] Endereço:Department of Chemistry, University of Engineering and Technology, Lahore, Pakistan; Department of Polymer Engineering and Technology, University of the Punjab, Lahore, Pakistan.
[Ti] Título:Development of a novel pH sensitive silane crosslinked injectable hydrogel for controlled release of neomycin sulfate.
[So] Source:Int J Biol Macromol;97:218-227, 2017 Apr.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Silane crosslinked biopolymer based novel pH-responsive hydrogels were fabricated by blending the cationic (chitosan) and anionic (alginate) polymers with poly(vinyl alcohol). Tetraethoxysilane (TEOS) was used, as a crosslinker in different amounts due to its nonhazardous nature, to study its impact on physical and chemical properties of the prepared injectable hydrogels along with the controlled release of drug. The swelling response of the prepared hydrogels was examined in different solvent media which exhibited decreased swelling ratio with increase in the amount of TEOS. All the fabricated hydrogels represented highest swelling at acidic pH while low swelling at basic and neutral pH. This specific pH sensitive behavior at pH 7 made them an appropriate candidate for the injectable controlled drug delivery in which Neomycin Sulfate (NMS) was successfully loaded on suitable hydrogel (comprising 50µL TEOS) to study its release mechanism. The results revealed that in simulated gastric fluid (SGF), hydrogel released the entire drug (NMS) in initial 30min while in simulated intestinal fluid (SIF), NMS was released in a controlled way up to 83% in 80min. These results endorsed that the hydrogels could be practiced as a smart intelligent material for injectable controlled drug delivery as well as for other biomedical applications at physiological pH.
[Mh] Termos MeSH primário: Portadores de Fármacos/química
Hidrogéis/química
Neomicina/química
Silanos/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Antibacterianos/toxicidade
Tampões (Química)
Preparações de Ação Retardada
Portadores de Fármacos/farmacologia
Portadores de Fármacos/toxicidade
Células HeLa
Seres Humanos
Hidrogéis/farmacologia
Hidrogéis/toxicidade
Injeções
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Buffers); 0 (Delayed-Action Preparations); 0 (Drug Carriers); 0 (Hydrogels); 0 (Silanes); 059QF0KO0R (Water); 1404-04-2 (Neomycin); 42064KRE49 (tetraethoxysilane)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170109
[St] Status:MEDLINE


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[PMID]:27878810
[Au] Autor:Marie D; Le Gall F; Edern R; Gourvil P; Vaulot D
[Ad] Endereço:UPMC Université Paris 06, CNRS, UMR7144, Station Biologique de Roscoff, Sorbonne Universités, Roscoff, France.
[Ti] Título:Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry.
[So] Source:J Phycol;53(2):271-282, 2017 Apr.
[Is] ISSN:1529-8817
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flow cytometry provides a tool to physically sort single algal cells in order to obtain clonal cultures. During sorting, cells are submitted to physical stress factors such as high fluidic pressure, exposure to the laser beam, electrostatic charges, deflection through high voltage fields, and collisions with container surfaces. All of these can damage the cells of interest and success rates for initiation of cultures from flow-sorted cells are generally very low. We found that the addition of bovine serum albumin in the culture medium into which cells were sorted drastically improved the success of initiation of pico- and nano-eukaryotic phytoplankton strains. Adding a mixture of antibiotics (Penicillin, Neomycin, Streptomycin) to the medium in order to slow down bacterial growth further improved culture development. This approach was successfully used to isolate taxonomically diverse strains, including novel taxa, from a fresh sample obtained in the English Channel and from enrichment cultures established during an Atlantic meridional transect cruise. We anticipate that these improvements will be useful to clone or purify existing cultures and to isolate novel cultures from oceanic samples.
[Mh] Termos MeSH primário: Fitoplâncton/citologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Citometria de Fluxo
Neomicina/farmacologia
Penicilinas/farmacologia
Fitoplâncton/efeitos dos fármacos
Estreptomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Penicillins); 1404-04-2 (Neomycin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.1111/jpy.12495



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