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[PMID]:28470446
[Au] Autor:Sundaran PS; Bhaskaran A; Alex ST; Prasad T; Haritha VH; Anie Y; Kumary TV; Anil Kumar PR
[Ad] Endereço:Division of Tissue Culture, Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, 695 012, India.
[Ti] Título:Drug loaded microbeads entrapped electrospun mat for wound dressing application.
[So] Source:J Mater Sci Mater Med;28(6):88, 2017 Jun.
[Is] ISSN:1573-4838
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new design of antibiotic loaded wound dressing and its initial in vitro evaluation is described. Chitosan microbeads loaded with ampicillin were sandwiched within polycaprolactone electrospun mat (MbAPPCL). The morphology was analyzed by scanning electron microscopy and surface chemistry was characterized by Fourier Transform Infrared Spectroscopy. In vitro cytotoxicity using L-929 fibroblast cells by direct contact test and elution assay revealed non-cytotoxic nature of MbAPPCL. The cell adhesion and viability analysis further confirmed the cytocompatibility of MbAPPCL as a wound dressing material. Percentage hemolysis and platelet adhesion on the mat exposed to blood substantiated the hemocompatibility. The antibiotic susceptibility test analyzed on Staphylococcus aureus by agar plate method confirmed the drug release and antimicrobial property. The proposed wound dressing model explained with ampicillin as a candidate drug has the potential to include microbeads with different antibiotics for multi drug treatment.
[Mh] Termos MeSH primário: Bandagens
Portadores de Fármacos
Microesferas
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Materiais Biocompatíveis
Plaquetas
Linhagem Celular
Quitosana
Técnicas Eletroquímicas
Fibroblastos/fisiologia
Teste de Materiais
Camundongos
Penicilinas/química
Penicilinas/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Estreptomicina/química
Estreptomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biocompatible Materials); 0 (Drug Carriers); 0 (Penicillins); 9012-76-4 (Chitosan); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10856-017-5893-8


  2 / 10523 MEDLINE  
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[PMID]:27776591
[Au] Autor:Lopez AL; Aldaba JG; Ama CG; Sylim PG; Geraldino XD; Sarol JN; Salonga AM
[Ad] Endereço:National Institutes of Health, University of the Philippines Manila, Manila, The Philippines.
[Ti] Título:Surveillance for tuberculosis in a rural community in The Philippines.
[So] Source:Int J Tuberc Lung Dis;20(11):1495-1500, 2016 Nov.
[Is] ISSN:1815-7920
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:SETTING: Estimates of the tuberculosis (TB) burden in the Philippines are largely dependent on prevalence surveys. OBJECTIVE: To conduct a prospective community-based survey to generate epidemiological data on TB among patients seeking care in public health centres in a rural municipality in the Philippines. DESIGN: Prospective surveillance and follow-up of presumptive TB cases from May 2013 to July 2015. RESULTS: Of 1622 participants with presumptive TB, 468 (28.8%) (95%CI 26.6-31.1) were diagnosed with TB. The annual TB case notification rate in San Juan was 212 (95%CI 184-242) per 100 000 population. There were nine TB-attributable deaths during the study period. Only 8.8% (95%CI 6.2-11.32) of the cases were children aged <15 years; 274 (58.5%) cases were bacteriologically confirmed. Of 210 isolates tested for antimicrobial resistance, 49 (23.3%, 95%CI 17.58-29.02) were resistant. Resistance to isoniazid (INH) was common (n = 33, 15.7%); multidrug-resistant TB was 1.9%. CONCLUSION: TB remains an important health problem in the Philippines. We identified low case detection of TB in children and high INH resistance rates in this rural community.
[Mh] Termos MeSH primário: Vigilância da População
População Rural
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
Tuberculose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Antituberculosos/uso terapêutico
Criança
Pré-Escolar
Feminino
Seguimentos
Seres Humanos
Lactente
Recém-Nascido
Isoniazida/uso terapêutico
Canamicina/uso terapêutico
Masculino
Meia-Idade
Filipinas/epidemiologia
Prevalência
Estudos Prospectivos
Saúde Pública
Rifampina/uso terapêutico
Estreptomicina/uso terapêutico
Resultado do Tratamento
Tuberculose/diagnóstico
Tuberculose/tratamento farmacológico
Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico
Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 59-01-8 (Kanamycin); V83O1VOZ8L (Isoniazid); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28951283
[Au] Autor:Wälti MA; Clore GM
[Ad] Endereço:Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, United States.
[Ti] Título:Disassembly/reassembly strategy for the production of highly pure GroEL, a tetradecameric supramolecular machine, suitable for quantitative NMR, EPR and mutational studies.
[So] Source:Protein Expr Purif;142:8-15, 2018 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:GroEL, a prototypical member of the chaperonin class of chaperones, is a large supramocular machine that assists protein folding and plays an important role in proteostasis. GroEL comprises two heptameric rings, each of which encloses a large cavity that provides a folding chamber for protein substrates. Many questions remain regarding the mechanistic details of GroEL facilitated protein folding. Thus, data at atomic resolution of the type provided by NMR and EPR are invaluable. Such studies often require complete deuteration of GroEL, uniform or residue specific C and N isotope labeling, and the introduction of selective cysteine mutations for site-specific spin labeling. In addition, high purity GroEL is essential for detailed studies of substrate-GroEL interactions as quantitative interpretation is impossible if the cavities are already occupied and blocked by other protein substrates present in the bacterial expression system. Here we present a new purification protocol designed to provide highly pure GroEL devoid of non-specific protein substrate contamination.
[Mh] Termos MeSH primário: Chaperonina 60/isolamento & purificação
Cromatografia em Gel/métodos
Cromatografia por Troca Iônica/métodos
Proteínas de Escherichia coli/isolamento & purificação
Mutação Puntual
[Mh] Termos MeSH secundário: Sulfato de Amônio/química
Chaperonina 60/química
Chaperonina 60/genética
Chaperonina 60/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Expressão Gênica
Isótopos de Nitrogênio/química
Ressonância Magnética Nuclear Biomolecular
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Estreptomicina/química
Ureia/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chaperonin 60); 0 (Escherichia coli Proteins); 0 (Nitrogen Isotopes); 0 (Recombinant Proteins); 8W8T17847W (Urea); SU46BAM238 (Ammonium Sulfate); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE


  4 / 10523 MEDLINE  
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[PMID]:28841532
[Au] Autor:Zhang X; Qi S; Liu C; Zhao X
[Ad] Endereço:Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi'an 710129, China; Department of Pharmacy, Xi'an Medical University, Xi'an 710021, China.
[Ti] Título:Enantiomeric separation of five acidic drugs via capillary electrophoresis using streptomycin as chiral selector.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:31-35, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple capillary zone electrophoresis (CZE) method was developed to achieve the rapid enantiomeric separation of a set of acidic drugs using streptomycin as chiral selector. The enantiomers of 5 chiral phenyl-containing acidic pharmaceutical compounds were separated excellently by CE using an uncoated silica capillary. Several experimental parameters such as the concentration of streptomycin, buffer concentration and pH, running voltage, and capillary temperature were all investigated systematically in order to optimize the chiral separation. All analytes were got baseline separation within 10min. And the results showed that streptomycin can be used as a chiral selector to the enantioseparation of five acidic drugs by CZE method.
[Mh] Termos MeSH primário: Eletroforese Capilar/métodos
Preparações Farmacêuticas/isolamento & purificação
Estreptomicina/química
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Preparações Farmacêuticas/análise
Preparações Farmacêuticas/química
Estereoisomerismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pharmaceutical Preparations); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  5 / 10523 MEDLINE  
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[PMID]:28808159
[Au] Autor:Machelart A; Khadrawi A; Demars A; Willemart K; De Trez C; Letesson JJ; Muraille E
[Ad] Endereço:Unité de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Université de Namur, Namur, Belgium.
[Ti] Título:Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen.
[So] Source:Infect Immun;85(11), 2017 Nov.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The spleen is known as an important filter for blood-borne pathogens that are trapped by specialized macrophages in the marginal zone (MZ): the CD209 MZ macrophages (MZMs) and the CD169 marginal metallophilic macrophages (MMMs). Acute systemic infection strongly impacts MZ populations and the location of T and B lymphocytes. This phenomenon has been linked to reduced chemokine secretion by stromal cells. spp. are the causative agent of brucellosis, a widespread zoonotic disease. Here, we used infection as a model to investigate the impact of chronic stealth infection on splenic MZ macrophage populations. During the late phase of infection, we observed a loss of both MZMs and MMMs, with a durable disappearance of MZMs, leading to a reduction of the ability of the spleen to take up soluble antigens, beads, and unrelated bacteria. This effect appears to be selective as every other lymphoid and myeloid population analyzed increased during infection, which was also observed following and infection. Comparison of wild-type and deficient mice suggested that MZ macrophage population loss is dependent on interferon gamma (IFN-γ) receptor but independent of T cells or tumor necrosis factor alpha receptor 1 (TNF-αR1) signaling pathways and is not correlated to an alteration of CCL19, CCL21, and CXCL13 chemokine mRNA expression. Our results suggest that MZ macrophage populations are particularly sensitive to persistent low-level IFN-γ-mediated inflammation and that infection could reduce the ability of the spleen to perform certain MZM- and MMM-dependent tasks, such as antigen delivery to lymphocytes and control of systemic infection.
[Mh] Termos MeSH primário: Brucelose/imunologia
Interações Hospedeiro-Patógeno
Interferon gama/imunologia
Macrófagos/imunologia
Receptores de Interferon/imunologia
Baço/imunologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Linfócitos B/imunologia
Linfócitos B/microbiologia
Brucella abortus/efeitos dos fármacos
Brucella abortus/imunologia
Brucella abortus/patogenicidade
Brucella melitensis/efeitos dos fármacos
Brucella melitensis/imunologia
Brucella melitensis/patogenicidade
Brucella suis/efeitos dos fármacos
Brucella suis/imunologia
Brucella suis/patogenicidade
Brucelose/tratamento farmacológico
Brucelose/genética
Brucelose/microbiologia
Quimiocina CCL19/genética
Quimiocina CCL19/imunologia
Quimiocina CCL21/genética
Quimiocina CCL21/imunologia
Quimiocina CXCL13/genética
Quimiocina CXCL13/imunologia
Doença Crônica
Regulação da Expressão Gênica
Interferon gama/genética
Macrófagos/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
RNA Mensageiro/genética
RNA Mensageiro/imunologia
Receptores de Interferon/deficiência
Receptores de Interferon/genética
Receptores Tipo I de Fatores de Necrose Tumoral/deficiência
Receptores Tipo I de Fatores de Necrose Tumoral/genética
Receptores Tipo I de Fatores de Necrose Tumoral/imunologia
Rifampina/farmacologia
Transdução de Sinais
Baço/microbiologia
Estreptomicina/farmacologia
Linfócitos T/imunologia
Linfócitos T/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Ccl19 protein, mouse); 0 (Chemokine CCL19); 0 (Chemokine CCL21); 0 (Chemokine CXCL13); 0 (Cxcl13 protein, mouse); 0 (RNA, Messenger); 0 (Receptors, Interferon); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (interferon gamma receptor); 82115-62-6 (Interferon-gamma); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  6 / 10523 MEDLINE  
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[PMID]:28757138
[Au] Autor:Mehta H; Goulet PO; Mashiko S; Desjardins J; Pérez G; Koenig M; Senécal JL; Constante M; Santos MM; Sarfati M
[Ad] Endereço:Immunoregulation Laboratory, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, Québec, Canada.
[Ti] Título:Early-Life Antibiotic Exposure Causes Intestinal Dysbiosis and Exacerbates Skin and Lung Pathology in Experimental Systemic Sclerosis.
[So] Source:J Invest Dermatol;137(11):2316-2325, 2017 Nov.
[Is] ISSN:1523-1747
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Patients with systemic sclerosis (SSc) display altered intestinal microbiota. However, the influence of intestinal dysbiosis on the development of experimental SSc remains unknown. Topoisomerase I peptide-loaded dendritic cell immunization induces SSc-like disease, with progressive skin and lung fibrosis. Breeders were given streptomycin and pups continued to receive antibiotic (ATB) until endpoint (lifelongATB). Alternately, ATB was withdrawn (earlyATB) or initiated (adultATB) during adulthood. Topoisomerase I peptide-loaded dendritic cell (no ATB) immunization induced pronounced skin fibrosis, with increased matrix (Col1a1), profibrotic (Il13, Tweakr), and vascular function (Serpine1) gene expression. Remarkably, earlyATB exposure was sufficient to augment skin Col5a1 and Il13 expression, and inflammatory cell infiltration, which included IL-13 cells, mononuclear phagocytes, and mast cells. Moreover, skin pathology exacerbation was also observed in lifelongATB and adultATB groups. Oral streptomycin administration induced intestinal dysbiosis, with exposure limited to early life (earlyATB) being sufficient to cause long-term modification of the microbiota and a shift toward increased Bacteroidetes/Firmicutes ratio. Finally, aggravated lung fibrosis and dysregulated pulmonary T-cell responses were observed in earlyATB and lifelongATB but not adultATB-exposed mice. Collectively, intestinal microbiota manipulation with streptomycin exacerbated pathology in two distinct sites, skin and lungs, with early life being a critical window to affect the course of SSc-like disease.
[Mh] Termos MeSH primário: Disbiose/genética
Microbioma Gastrointestinal/efeitos dos fármacos
Fibrose Pulmonar/patologia
Escleroderma Sistêmico/genética
Escleroderma Sistêmico/imunologia
Estreptomicina/farmacologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Células Cultivadas
DNA Bacteriano/análise
Células Dendríticas/efeitos dos fármacos
Modelos Animais de Doenças
Disbiose/microbiologia
Feminino
Microbioma Gastrointestinal/genética
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Transgênicos
Fibrose Pulmonar/genética
Distribuição Aleatória
Reação em Cadeia da Polimerase em Tempo Real/métodos
Fatores de Risco
Escleroderma Sistêmico/patologia
Estatísticas não Paramétricas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  7 / 10523 MEDLINE  
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[PMID]:28628661
[Au] Autor:Wang Y; Li J; Xiong K; Chen Z; Zheng C; Tan Y; Cong Y
[Ad] Endereço:Department of Microbiology, Third Military Medical University, Chongqing, China.
[Ti] Título:Elimination of persistent vaccine bacteria of Salmonella enterica serovar Typhimurium in the guts of immunized mice by inducible expression of truncated YncE.
[So] Source:PLoS One;12(6):e0179649, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Orally administered vaccine bacteria usually persist for a period of time in the intestinal tracts of immunized individuals, and are excreted in feces to the environment resulting in a potential biosafety issue. The releasing risk can be minimized by immediate elimination of the persistent vaccine bacteria once adequate protective immune responses have been elicited by the vaccine bacteria. In a previous study, inducible expression of truncated yncE gene (yncE*) was found lethal to host bacteria. This feature has an application potential in biosafety control. Here, we assessed the efficacy of YncE* in eliminating an attenuated strain of Salmonella enterica serovar Typhimurium in a mouse model. To this end, a pBAD-derived plasmid containing yncE* under the control of the Ara promoter was transformed into a ΔphoPQ mutant of S. Typhimurium. Our data show that the induced expression of yncE* in the presence of arabinose eliminated the vaccine bacteria both in vitro and in vivo. BALB/c mice with or without streptomycin-pretreatment were used to assess the efficacy of YncE* in vivo. Oral administration of 500 µl of 20% arabinose at 24 h postvaccination removed the vaccine bacteria from the guts of the tested mice without streptomycin-pretreatment. For streptomycin-pretreated mice, which were colonized with higher levels of Salmonella, an additional gavage of arabinose was required to completely eliminate the vaccine bacteria in the guts of the tested mice. The orally administered arabinose did not affect the persistence of bacteria that had penetrated the intestinal mucosa of the immunized mice. Furthermore, there was no significant difference in the protection rate between the routine immunization and the immunization with the arabinose treatment. The results indicate that the yncE* element improves the biosafety of the bacterial vaccine, and can be taken in consideration in future design of live bacterial vaccines.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Infecções por Salmonella/prevenção & controle
Vacinas contra Salmonella/imunologia
Salmonella typhimurium/metabolismo
Vacinas Atenuadas/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Arabinose/farmacologia
Proteínas de Bactérias/genética
Modelos Animais de Doenças
Feminino
Expressão Gênica/efeitos dos fármacos
Intestinos/microbiologia
Dose Letal Mediana
Lipopolissacarídeos/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Plasmídeos/genética
Plasmídeos/metabolismo
Infecções por Salmonella/microbiologia
Salmonella typhimurium/efeitos dos fármacos
Estreptomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Bacterial Proteins); 0 (Lipopolysaccharides); 0 (PhoQ protein, Bacteria); 0 (Salmonella Vaccines); 0 (Vaccines, Attenuated); 125360-99-8 (PhoP protein, Bacteria); B40ROO395Z (Arabinose); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179649


  8 / 10523 MEDLINE  
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[PMID]:28419091
[Au] Autor:Moura de Sousa J; Balbontín R; Durão P; Gordo I
[Ad] Endereço:Instituto Gulbenkian de Ciência, Oeiras, Portugal.
[Ti] Título:Multidrug-resistant bacteria compensate for the epistasis between resistances.
[So] Source:PLoS Biol;15(4):e2001741, 2017 Apr.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutations conferring resistance to antibiotics are typically costly in the absence of the drug, but bacteria can reduce this cost by acquiring compensatory mutations. Thus, the rate of acquisition of compensatory mutations and their effects are key for the maintenance and dissemination of antibiotic resistances. While compensation for single resistances has been extensively studied, compensatory evolution of multiresistant bacteria remains unexplored. Importantly, since resistance mutations often interact epistatically, compensation of multiresistant bacteria may significantly differ from that of single-resistant strains. We used experimental evolution, next-generation sequencing, in silico simulations, and genome editing to compare the compensatory process of a streptomycin and rifampicin double-resistant Escherichia coli with those of single-resistant clones. We demonstrate that low-fitness double-resistant bacteria compensate faster than single-resistant strains due to the acquisition of compensatory mutations with larger effects. Strikingly, we identified mutations that only compensate for double resistance, being neutral or deleterious in sensitive or single-resistant backgrounds. Moreover, we show that their beneficial effects strongly decrease or disappear in conditions where the epistatic interaction between resistance alleles is absent, demonstrating that these mutations compensate for the epistasis. In summary, our data indicate that epistatic interactions between antibiotic resistances, leading to large fitness costs, possibly open alternative paths for rapid compensatory evolution, thereby potentially stabilizing costly multiple resistances in bacterial populations.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana/genética
Farmacorresistência Bacteriana Múltipla/genética
Epistasia Genética
Escherichia coli/genética
Mutação
[Mh] Termos MeSH secundário: Alelos
Antibacterianos/farmacologia
Sequência de Bases
Evolução Molecular Direcionada
Escherichia coli/efeitos dos fármacos
Proteínas de Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Aptidão Genética/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Fatores de Alongamento de Peptídeos/genética
Sequências Reguladoras de Ácido Nucleico/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas Ribossômicas/genética
Rifampina/farmacologia
Estreptomicina/farmacologia
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Escherichia coli Proteins); 0 (NusG protein, E coli); 0 (Peptide Elongation Factors); 0 (Ribosomal Proteins); 0 (Transcription Factors); 0 (ribosomal protein S10); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2001741


  9 / 10523 MEDLINE  
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[PMID]:28416581
[Au] Autor:Goralczyk A; van Vijven M; Koch M; Badowski C; Yassin MS; Toh SA; Shabbir A; Franco-Obregón A; Raghunath M
[Ad] Endereço:Department of Biomedical Engineering, National University of Singapore, Singapore.
[Ti] Título:TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin.
[So] Source:FASEB J;31(8):3251-3266, 2017 Aug.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transient receptor potential (TRP) channels are polymodal cell sensors responding to diverse stimuli and widely implicated in the developmental programs of numerous tissues. The evidence for an involvement of TRP family members in adipogenesis, however, is scant. We present the first comprehensive expression profile of all known 27 human TRP genes in mesenchymal progenitors cells during white or brown adipogenesis. Using positive trilineage differentiation as an exclusion criterion, TRP polycystic (P)3, and TPR melastatin (M)8 were found to be uniquely adipospecific. Knockdown of TRPP3 repressed the expression of the brown fat signature genes uncoupling protein (UCP)-1 and peroxisome proliferator-activated receptor γ coactivator (PGC)-1α as well as attenuated forskolin-stimulated uncoupled respiration. However, indices of generalized adipogenesis, such as lipid droplet morphology and fatty acid binding protein (FAPB)-4 expression, were not affected, indicating a principal mitochondrial role of TRPP3. Conversely, activating TRPM8 with menthol up-regulated UCP-1 expression and augmented uncoupled respiration predominantly in white adipocytes (browning), whereas streptomycin antagonized TRPM8-mediated calcium entry, downregulated UCP-1 expression, and mitigated uncoupled respiration; menthol was less capable of augmenting uncoupled respiration (thermogenesis) in brown adipocytes. TRPP3 and TRPM8 hence appear to be involved in the priming of mitochondria to perform uncoupled respiration downstream of adenylate cyclase. Our results also underscore the developmental caveats of using antibiotics in adipogenic studies.-Goralczyk, A., van Vijven, M., Koch, M., Badowski, C., Yassin, M. S., Toh, S.-A., Shabbir, A., Franco-Obregón, A., Raghunath, M. TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin.
[Mh] Termos MeSH primário: Adipogenia/fisiologia
Tecido Adiposo Marrom/metabolismo
Tecido Adiposo Branco/metabolismo
Antibacterianos/efeitos adversos
Estreptomicina/efeitos adversos
Canais de Receptores Transientes de Potencial/metabolismo
[Mh] Termos MeSH secundário: Adulto
Canais de Cálcio/genética
Canais de Cálcio/metabolismo
Diferenciação Celular
Regulação da Expressão Gênica/fisiologia
Seres Humanos
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Isoformas de Proteínas
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Canais de Receptores Transientes de Potencial/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Calcium Channels); 0 (Membrane Proteins); 0 (PKD2L1 protein, human); 0 (Protein Isoforms); 0 (Receptors, Cell Surface); 0 (TRPM8 channel-associated factor 1 protein, human); 0 (Transient Receptor Potential Channels); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201601081RR


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[PMID]:28333978
[Au] Autor:Zhou Y; van den Hof S; Wang S; Pang Y; Zhao B; Xia H; Anthony R; Ou X; Li Q; Zheng Y; Song Y; Zhao Y; van Soolingen D
[Ad] Endereço:Chinese Centre for Disease Control and Prevention, Changping district, Beijing, China, P.R.
[Ti] Título:Association between genotype and drug resistance profiles of Mycobacterium tuberculosis strains circulating in China in a national drug resistance survey.
[So] Source:PLoS One;12(3):e0174197, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe the population structure of a representative collection of 3,133 Mycobacterium tuberculosis isolates, collected within the framework of a national resistance survey from 2007 in China. Genotyping data indicate that the epidemic strains in China can be divided into seven major complexes, of which 92% belonged to the East Asian (mainly Beijing strains) or the Euro-American lineage. The epidemic Beijing strains in China are closely related to the Beijing B0/W148 strain earlier described in Russia and a large cluster of these strains has spread national wide. The density of Beijing strains is high in the whole of China (average 70%), but the highest prevalence was found North of the Yellow river. The Euro-American lineage consists of three sublineages (sublineage_1, 2, and 3) and is more prevalent in the South. Beijing lineage showed the highest cluster rate of 48% and a significantly higher level of resistance to rifampicin (14%, p<0.001), ethambutol (9%, p = 0.001), and ofloxacin (5%, p = 0.011). Within the Euro-American Lineage, sublineage_3 revealed the highest cluster rate (28%) and presented a significantly elevated level of resistance to streptomycin (44%, p<0.001). Our findings suggest that standardised treatment in this region may have contributed to the successful spread of certain strains: sublineage_3 in the Euro-American lineage may have thrived when streptomycin was used without rifampicin for treatment, while later under DOTS based treatment, in which rifampicin plays a key role, Beijing lineage appears to be spreading.
[Mh] Termos MeSH primário: Antituberculosos/uso terapêutico
Mycobacterium tuberculosis/genética
Tuberculose Resistente a Múltiplos Medicamentos/genética
[Mh] Termos MeSH secundário: China/epidemiologia
Genótipo
Seres Humanos
Testes de Sensibilidade Microbiana
Repetições Minissatélites/genética
Mycobacterium tuberculosis/efeitos dos fármacos
Rifampina/uso terapêutico
Estreptomicina/uso terapêutico
Inquéritos e Questionários
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174197



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