Base de dados : MEDLINE
Pesquisa : D09.408.620 [Categoria DeCS]
Referências encontradas : 17271 [refinar]
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  1 / 17271 MEDLINE  
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[PMID]:28467092
[Au] Autor:Wu M; Ye H; Shao C; Zheng X; Li Q; Wang L; Zhao M; Lu G; Chen B; Zhang J; Wang Y; Wang G; Hao H
[Ad] Endereço:Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines and ‡School of Pharmacy, China Pharmaceutical University , Tongjiaxiang #24, Nanjing 210009, China.
[Ti] Título:Metabolomics-Proteomics Combined Approach Identifies Differential Metabolism-Associated Molecular Events between Senescence and Apoptosis.
[So] Source:J Proteome Res;16(6):2250-2261, 2017 Jun 02.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apoptosis and senescence are two types of cell fates in response to chemotherapy. Besides canonical pathways that mediate cell fates, cancer cell metabolism has been revealed as a crucial factor affecting cell fate decisions and thus represents a new target for antitumor therapy. Therefore, a comprehensive description of metabolic pathways underlying cell senescence and apoptosis in response to chemotherapy is highly demanded for therapeutic exploitation of both processes. Herein we employed a metabolomics-proteomics combined approach to identify metabolism-associated molecular events that mediate cellular responses to senescence and apoptosis using doxorubicin-treated human breast cancer cells MCF7 as models. Such biomics approach revealed that tricarboxylic acid cycle, pentose phosphate pathway, and nucleotide synthesis pathways were significantly upregulated in the senescent model, whereas fatty acid synthesis was reduced. In apoptotic cells, an overall reduced activity of major metabolic pathways was observed except for the arginine and proline pathway. Combinatorially, these data show the utility of biomics in exploring biochemical mechanism-based differences between apoptosis and senescence and reveal an unprecedented finding of the metabolic events that were induced for survival by facilitating ROS elimination and DNA damage repair in senescent cells, while they were downregulated in apoptotic cells when DNA damage was irreparable.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Senescência Celular/efeitos dos fármacos
Redes e Vias Metabólicas/efeitos dos fármacos
Metabolômica/métodos
Proteômica/métodos
[Mh] Termos MeSH secundário: Ciclo do Ácido Cítrico
Dano ao DNA
Doxorrubicina/farmacologia
Doxorrubicina/uso terapêutico
Ácidos Graxos/biossíntese
Seres Humanos
Células MCF-7
Nucleotídeos/biossíntese
Via de Pentose Fosfato
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Nucleotides); 0 (Reactive Oxygen Species); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jproteome.7b00111


  2 / 17271 MEDLINE  
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[PMID]:28454775
[Au] Autor:Eggert F; Kulikov K; Domnick C; Leifels P; Kath-Schorr S
[Ad] Endereço:LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany.
[Ti] Título:Iluminated by foreign letters - Strategies for site-specific cyclopropene modification of large functional RNAs via in vitro transcription.
[So] Source:Methods;120:17-27, 2017 May 01.
[Is] ISSN:1095-9130
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The synthesis of sequence-specifically modified long RNA molecules, which cannot entirely be prepared via solid phase synthesis methods is experimentally challenging. We are using a new approach based on an expanded genetic alphabet preparing site-specifically modified RNA molecules via standard in vitro transcription. In this report, the site-specific labeling of functional RNAs, in particular ribozymes and a long non-coding RNA with cyclopropene moieties, is presented. We provide detailed instructions for RNA labeling via in vitro transcription and include required analytical methods to verify production and identity of the transcript. We further present post-transcriptional inverse electron demand Diels-Alder cycloaddition reactions on the cyclopropene-modified sequences and discuss applications of the genetic alphabet expansion transcription for in vitro preparation of labeled functional RNAs with complex foldings. In detail, the glmS and CPEB3 ribozymes were site-specifically decorated with methyl cyclopropene moieties using the unnatural TPT3 triphosphate and were proven to be still functional. In addition, the structurally complex A region of the Xist lncRNA (401nt) was site-specifically modified with methyl cyclopropene and detected by fluorescence after cycloaddition reaction with a tetrazine-BODIPY conjugate.
[Mh] Termos MeSH primário: Reação de Cicloadição/métodos
Ciclopropanos/química
RNA Catalítico/química
RNA Longo não Codificante/química
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Elétrons
Corantes Fluorescentes/química
Técnicas In Vitro/métodos
Nucleotídeos/química
Processamento Pós-Transcricional do RNA
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopropanes); 0 (Fluorescent Dyes); 0 (Nucleotides); 0 (RNA, Catalytic); 0 (RNA, Long Noncoding); J6UJO23JGU (1-methylcyclopropene)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  3 / 17271 MEDLINE  
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[PMID]:29386460
[Au] Autor:Shuto S
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Hokkaido University.
[Ti] Título:Foreword.
[So] Source:Chem Pharm Bull (Tokyo);66(2):116, 2018.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Antivirais/farmacologia
Nucleosídeos/farmacologia
Nucleotídeos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Antivirais/síntese química
Antivirais/química
Pesquisa Biomédica
Química Farmacêutica
Sistemas de Liberação de Medicamentos
Seres Humanos
Nucleosídeos/síntese química
Nucleosídeos/química
Nucleotídeos/síntese química
Nucleotídeos/química
[Pt] Tipo de publicação:INTRODUCTORY JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antiviral Agents); 0 (Nucleosides); 0 (Nucleotides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c18-ctf6602


  4 / 17271 MEDLINE  
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[PMID]:29334633
[Au] Autor:Kiyokawa E; Hayama T; Yoshida H; Yamaguchi M; Nohta H
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan.
[Ti] Título:Fluorous-assisted metal chelate affinity extraction for nucleotides followed by HILIC-MS/MS analysis.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:86-90, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We herein developed a selective method for the determination of nucleotides by fluorous-assisted metal chelate affinity extraction followed by hydrophilic interaction liquid chromatography (HILIC) combined with tandem mass spectrometric (MS/MS) analysis. In this study, the nucleotides were selectively chelated by Fe(III)-immobilized perfluoroalkyliminodiacetic acid, and the resulting chelates were subsequently extracted into a fluorous solvent. The nucleotides present in the fluorous solvent were then back-extracted into a non-fluorous solution, such as a solution of ammonia in aqueous acetonitrile. The resulting non-fluorous solution containing the nucleotides was then directly injected into an amide-type HILIC column using a mixture of acetonitrile and aqueous ammonium bicarbonate as the mobile phase for gradient elution, and the nucleotides were detected using the negative electrospray ionization MS/MS mode. In this method, the extraction recoveries of the nucleotides ranged from 43.2 to 94.7% within a relative standard deviation of 17%. This method enabled the determination of intracellular concentrations of nucleotides.
[Mh] Termos MeSH primário: Quelantes/química
Cromatografia Líquida/métodos
Fluoretos/química
Nucleotídeos/análise
Nucleotídeos/isolamento & purificação
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Células Jurkat
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Nucleotides); Q80VPU408O (Fluorides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180116
[St] Status:MEDLINE


  5 / 17271 MEDLINE  
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[PMID]:29265824
[Au] Autor:Mlýnský V; Bussi G
[Ad] Endereço:Scuola Internazionale Superiore di Studi Avanzati, SISSA , via Bonomea 265, 34136 Trieste, Italy.
[Ti] Título:Molecular Dynamics Simulations Reveal an Interplay between SHAPE Reagent Binding and RNA Flexibility.
[So] Source:J Phys Chem Lett;9(2):313-318, 2018 Jan 18.
[Is] ISSN:1948-7185
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The function of RNA molecules usually depends on their overall fold and on the presence of specific structural motifs. Chemical probing methods are routinely used in combination with nearest-neighbor models to determine RNA secondary structure. Among the available methods, SHAPE is relevant due to its capability to probe all RNA nucleotides and the possibility to be used in vivo. However, the structural determinants for SHAPE reactivity and its mechanism of reaction are still unclear. Here molecular dynamics simulations and enhanced sampling techniques are used to predict the accessibility of nucleotide analogs and larger RNA structural motifs to SHAPE reagents. We show that local RNA reconformations are crucial in allowing reagents to reach the 2'-OH group of a particular nucleotide and that sugar pucker is a major structural factor influencing SHAPE reactivity.
[Mh] Termos MeSH primário: Simulação de Dinâmica Molecular
Nucleotídeos
Dobramento de RNA
RNA/química
[Mh] Termos MeSH secundário: Modelos Moleculares
Conformação de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleotides); 63231-63-0 (RNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180304
[Lr] Data última revisão:
180304
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jpclett.7b02921


  6 / 17271 MEDLINE  
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[PMID]:29289891
[Au] Autor:Szymanska-Michalak A; Wawrzyniak D; Framski G; Stawinski J; Barciszewski J; Kraszewski A
[Ad] Endereço:Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
[Ti] Título:New antiglioma zwitterionic pronucleotides with an FdUMP framework.
[So] Source:Eur J Med Chem;144:682-691, 2018 Jan 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:We have designed and synthesized new 5-fluoro-2'-deoxyuridine 5'-phosphate pronucleotides which can function as potential agents against the glioblastoma multiforme tumor. Their anti-malignant potency has been tested against T98G, U-118 MG, U-87 MG gliomas, HeLa, and Caco-2 cancer cell lines, using MRC-5 healthy cells as a reference. Five of the sixteen compounds (4c, 4f-i) exhibited significant anticancer potency and high selectivity indices (SI 12-66). It is likely that these zwitterionic pronucleotides may function in a similar manner to zwitterionic phospholipids, by inducing cell membrane charge disorder, making the cell permeable to bioactive agents. The most promising therapeutic pronucleotides 4c, 4f-h, have high intestinal-blood uptake potency (Caco-2 cell line), and may be considered as potential, orally administrated, anticancer drugs.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Citidina Monofosfato/análogos & derivados
Glioblastoma/tratamento farmacológico
Nucleotídeos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Citidina Monofosfato/química
Citidina Monofosfato/farmacologia
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Glioblastoma/patologia
Seres Humanos
Estrutura Molecular
Nucleotídeos/síntese química
Nucleotídeos/química
Relação Estrutura-Atividade
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Nucleotides); 847-22-3 (5-fluoro-2'-deoxycytidine 5'-monophosphate); F469818O25 (Cytidine Monophosphate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180101
[St] Status:MEDLINE


  7 / 17271 MEDLINE  
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[PMID]:29297910
[Au] Autor:Pedreira-Segade U; Michot LJ; Daniel I
[Ad] Endereço:Univ Lyon, Ens de Lyon, Université Lyon 1, CNRS, UMR 5276 LGL-TPE, F-69342, Lyon, France. ulysse.pedreira@ens-lyon.org.
[Ti] Título:Effects of salinity on the adsorption of nucleotides onto phyllosilicates.
[So] Source:Phys Chem Chem Phys;20(3):1938-1952, 2018 Jan 17.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the context of the origin of life, phyllosilicate surfaces might favor the adsorption, concentration and reactivity of otherwise diluted prebiotic molecules. The primitive oceanic seafloor was certainly rich in Fe-Mg-rich phyllosilicates. The salinity of the primitive seawater remains largely unknown. Values ranging from 1 to 15 times modern salinity have been proposed and the salt composition of the primitive ocean also remains elusive although it may have played a role in the interactions between nucleotides and mineral surfaces. Therefore we studied the adsorption of 5'-monophosphate deoxyguanosine (dGMP) as a model nucleotide onto a Fe-rich swelling clay, i.e. nontronite, and an Al-rich phyllosilicate, i.e. pyrophyllite, for comparison. Experiments were carried out at atmospheric pressure, 25 °C and natural pH, with a series of salts NaCl, MgCl , CaCl , MgSO , NaH PO and LaCl in order to evaluate the effect of cations and anions on dGMP adsorption. The present study shows that nucleotides are adsorbed on both phyllosilicates via a ligand exchange mechanism. The phosphate group of the nucleotide is adsorbed on the lateral metal hydroxyls of the broken edges of phyllosilicates. The presence of divalent cations or molecular anions, such as phosphate or sulfate, tends to inhibit this interaction on mineral surfaces. However, in the presence of divalent cations, cationic bridging on the basal surfaces of the swelling clay also occurs and could induce a higher retention capacity of the swelling clays compared to non-swelling phyllosilicates in primitive and modern natural environments.
[Mh] Termos MeSH primário: Silicatos de Alumínio/química
Nucleotídeos/química
[Mh] Termos MeSH secundário: Adsorção
Cátions Bivalentes/química
Nucleotídeos de Desoxiguanina/química
Concentração de Íons de Hidrogênio
Sais/química
Espalhamento a Baixo Ângulo
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Cations, Divalent); 0 (Deoxyguanine Nucleotides); 0 (Nucleotides); 0 (Salts); 1302-87-0 (clay); 7EAM4TG712 (2'-deoxyguanosine 5'-phosphate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07004g


  8 / 17271 MEDLINE  
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[PMID]:29254895
[Au] Autor:Xu P; Feng X; Luan H; Wang J; Ge R; Li Z; Bian J
[Ad] Endereço:Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, People's Republic of China; Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
[Ti] Título:Current knowledge on the nucleotide agonists for the P2Y2 receptor.
[So] Source:Bioorg Med Chem;26(2):366-375, 2018 01 15.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:P2Y receptors are G-protein-coupled receptors (GPCRs) for extracellular nucleotides. There are eight mammalian P2Y receptor subtypes (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and P2Y14). P2Y2 receptors are widely expressed and play important roles in multiple functionalities. Diquafosol tetrasodium, known as INS365, which was the first P2Y2 receptor agonists that had been approved in April 2010 and launched in Japan by Santen Pharmaceuticals. Besides, a series of similar agonists for the P2Y2 receptor are undergoing development to cure different diseases related to the P2Y2 receptor. This article illustrated the structure and functions of the P2Y2 receptor and focused on several kinds of agonists about their molecular structures, research progress and chemical synthesis methods. Last but not the least, we summarized the structures-activity relationship (SAR) of agonists for the P2Y2 receptor and expected more efficient agonists for the P2Y2 receptor.
[Mh] Termos MeSH primário: Nucleotídeos/farmacologia
Receptores Purinérgicos P2Y2/metabolismo
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Nucleotídeos/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nucleotides); 0 (Receptors, Purinergic P2Y2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  9 / 17271 MEDLINE  
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[PMID]:28462530
[Au] Autor:Blander JM
[Ad] Endereço:Jill Roberts Institute for Research in Inflammatory Bowel Disease, Joan and Sanford I. Weill Department of Medicine, Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, New York, NY, USA.
[Ti] Título:The many ways tissue phagocytes respond to dying cells.
[So] Source:Immunol Rev;277(1):158-173, 2017 05.
[Is] ISSN:1600-065X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Apoptosis is an important component of normal tissue physiology, and the prompt removal of apoptotic cells is equally essential to avoid the undesirable consequences of their accumulation and disintegration. Professional phagocytes are highly specialized for engulfing apoptotic cells. The recent ability to track cells that have undergone apoptosis in situ has revealed a division of labor among the tissue resident phagocytes that sample them. Macrophages are uniquely programmed to process internalized apoptotic cell-derived fatty acids, cholesterol and nucleotides, as a reflection of their dominant role in clearing the bulk of apoptotic cells. Dendritic cells carry apoptotic cells to lymph nodes where they signal the emergence and expansion of highly suppressive regulatory CD4 T cells. A broad suppression of inflammation is executed through distinct phagocyte-specific mechanisms. A clever induction of negative regulatory nodes is notable in dendritic cells serving to simultaneously shut down multiple pathways of inflammation. Several of the genes and pathways modulated in phagocytes in response to apoptotic cells have been linked to chronic inflammatory and autoimmune diseases such as atherosclerosis, inflammatory bowel disease and systemic lupus erythematosus. Our collective understanding of old and new phagocyte functions after apoptotic cell phagocytosis demonstrates the enormity of ways to mediate immune suppression and enforce tissue homeostasis.
[Mh] Termos MeSH primário: Aterosclerose/imunologia
Doenças Inflamatórias Intestinais/imunologia
Fagócitos/fisiologia
Fagocitose
[Mh] Termos MeSH secundário: Animais
Apoptose
Colesterol/metabolismo
Células Dendríticas/imunologia
Ácidos Graxos/metabolismo
Homeostase
Seres Humanos
Tolerância Imunológica
Lúpus Eritematoso Sistêmico
Nucleotídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Nucleotides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/imr.12537


  10 / 17271 MEDLINE  
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[PMID]:28747171
[Au] Autor:Fragomeni BO; Lourenco DAL; Masuda Y; Legarra A; Misztal I
[Ad] Endereço:Edgar L. Rhodes Center for Animal and Dairy Science, University of Georgia, Athens, GA, USA. fragomen@uga.edu.
[Ti] Título:Incorporation of causative quantitative trait nucleotides in single-step GBLUP.
[So] Source:Genet Sel Evol;49(1):59, 2017 07 26.
[Is] ISSN:1297-9686
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Much effort is put into identifying causative quantitative trait nucleotides (QTN) in animal breeding, empowered by the availability of dense single nucleotide polymorphism (SNP) information. Genomic selection using traditional SNP information is easily implemented for any number of genotyped individuals using single-step genomic best linear unbiased predictor (ssGBLUP) with the algorithm for proven and young (APY). Our aim was to investigate whether ssGBLUP is useful for genomic prediction when some or all QTN are known. METHODS: Simulations included 180,000 animals across 11 generations. Phenotypes were available for all animals in generations 6 to 10. Genotypes for 60,000 SNPs across 10 chromosomes were available for 29,000 individuals. The genetic variance was fully accounted for by 100 or 1000 biallelic QTN. Raw genomic relationship matrices (GRM) were computed from (a) unweighted SNPs, (b) unweighted SNPs and causative QTN, (c) SNPs and causative QTN weighted with results obtained with genome-wide association studies, (d) unweighted SNPs and causative QTN with simulated weights, (e) only unweighted causative QTN, (f-h) as in (b-d) but using only the top 10% causative QTN, and (i) using only causative QTN with simulated weight. Predictions were computed by pedigree-based BLUP (PBLUP) and ssGBLUP. Raw GRM were blended with 1 or 5% of the numerator relationship matrix, or 1% of the identity matrix. Inverses of GRM were obtained directly or with APY. RESULTS: Accuracy of breeding values for 5000 genotyped animals in the last generation with PBLUP was 0.32, and for ssGBLUP it increased to 0.49 with an unweighted GRM, 0.53 after adding unweighted QTN, 0.63 when QTN weights were estimated, and 0.89 when QTN weights were based on true effects known from the simulation. When the GRM was constructed from causative QTN only, accuracy was 0.95 and 0.99 with blending at 5 and 1%, respectively. Accuracies simulating 1000 QTN were generally lower, with a similar trend. Accuracies using the APY inverse were equal or higher than those with a regular inverse. CONCLUSIONS: Single-step GBLUP can account for causative QTN via a weighted GRM. Accuracy gains are maximum when variances of causative QTN are known and blending is at 1%.
[Mh] Termos MeSH primário: Cruzamento
Genoma/genética
Modelos Genéticos
Locos de Características Quantitativas/genética
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
Estudo de Associação Genômica Ampla
Genótipo
Nucleotídeos/genética
Fenótipo
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Nucleotides)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180218
[Lr] Data última revisão:
180218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1186/s12711-017-0335-0



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde