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| [PMID]: | 28279980 |
| [Au] Autor: | McMillin SL; Schmidt DL; Kahn BB; Witczak CA |
| [Ad] Endereço: | Department of Kinesiology, East Carolina University, Greenville, NC. |
| [Ti] Título: | GLUT4 Is Not Necessary for Overload-Induced Glucose Uptake or Hypertrophic Growth in Mouse Skeletal Muscle. |
| [So] Source: | Diabetes;66(6):1491-1500, 2017 Jun. | | [Is] ISSN: | 1939-327X |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | GLUT4 is necessary for acute insulin- and contraction-induced skeletal muscle glucose uptake, but its role in chronic muscle loading (overload)-induced glucose uptake is unknown. Our goal was to determine whether GLUT4 is required for overload-induced glucose uptake. Overload was induced in mouse plantaris muscle by unilateral synergist ablation. After 5 days, muscle weights and ex vivo [ H]-2-deoxy-d-glucose uptake were assessed. Overload-induced muscle glucose uptake and hypertrophic growth were not impaired in muscle-specific GLUT4 knockout mice, demonstrating that GLUT4 is not necessary for these processes. To assess which transporters mediate overload-induced glucose uptake, chemical inhibitors were used. The facilitative GLUT inhibitor cytochalasin B, but not the sodium-dependent glucose cotransport inhibitor phloridzin, prevented overload-induced uptake demonstrating that GLUTs mediate this effect. To assess which GLUT, hexose competition experiments were performed. Overload-induced [ H]-2-deoxy-d-glucose uptake was not inhibited by d-fructose, demonstrating that the fructose-transporting GLUT2, GLUT5, GLUT8, and GLUT12 do not mediate this effect. To assess additional GLUTs, immunoblots were performed. Overload increased GLUT1, GLUT3, GLUT6, and GLUT10 protein levels twofold to fivefold. Collectively, these results demonstrate that GLUT4 is not necessary for overload-induced muscle glucose uptake or hypertrophic growth and suggest that GLUT1, GLUT3, GLUT6, and/or GLUT10 mediate overload-induced glucose uptake. |
| [Mh] Termos MeSH primário: |
Transportador de Glucose Tipo 4/genética
Glucose/metabolismo
Músculo Esquelético/metabolismo
Suporte de Carga
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| [Mh] Termos MeSH secundário: |
Animais
Citocalasina B/farmacologia
Desoxiglucose/metabolismo
Frutose/farmacologia
Proteínas Facilitadoras de Transporte de Glucose/metabolismo
Transportador de Glucose Tipo 1/metabolismo
Transportador de Glucose Tipo 2
Transportador de Glucose Tipo 4/metabolismo
Hipertrofia/genética
Immunoblotting
Camundongos
Camundongos Knockout
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/patologia
Florizina/farmacologia
Trítio
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Glucose Transport Proteins, Facilitative); 0 (Glucose Transporter Type 1); 0 (Glucose Transporter Type 2); 0 (Glucose Transporter Type 4); 0 (Slc2A10 protein, mouse); 0 (Slc2a1 protein, mouse); 0 (Slc2a12 protein, mouse); 0 (Slc2a2 protein, mouse); 0 (Slc2a4 protein, mouse); 0 (Slc2a5 protein, mouse); 0 (Slc2a8 protein, mouse); 0 (Slc2a9 protein, mouse); 10028-17-8 (Tritium); 30237-26-4 (Fructose); 3CHI920QS7 (Cytochalasin B); 9G2MP84A8W (Deoxyglucose); CU9S17279X (Phlorhizin); IY9XDZ35W2 (Glucose) |
| [Em] Mês de entrada: | 1708 |
| [Cu] Atualização por classe: | 170822 |
[Lr] Data última revisão:
| 170822 |
| [Sb] Subgrupo de revista: | AIM; IM |
| [Da] Data de entrada para processamento: | 170311 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.2337/db16-1075 |
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