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Lajolo, Franco M
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[PMID]:28867106
[Au] Autor:Tobaruela EC; Santos AO; Almeida-Muradian LB; Araujo EDS; Lajolo FM; Menezes EW
[Ad] Endereço:Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil; Food Research Center (FoRC), São Paulo, SP, Brazil; Food and Nutrition Research Center (NAPAN) - USP, Brazil.
[Ti] Título:Application of dietary fiber method AOAC 2011.25 in fruit and comparison with AOAC 991.43 method.
[So] Source:Food Chem;238:87-93, 2018 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AOAC 2011.25 method enables the quantification of most of the dietary fiber (DF) components according to the definition proposed by Codex Alimentarius. This study aimed to compare the DF content in fruits analyzed by the AOAC 2011.25 and AOAC 991.43 methods. Plums (Prunus salicina), atemoyas (Annona x atemoya), jackfruits (Artocarpus heterophyllus), and mature coconuts (Cocos nucifera) from different Brazilian regions (3 lots/fruit) were analyzed for DF, resistant starch, and fructans contents. The AOAC 2011.25 method was evaluated for precision, accuracy, and linearity in different food matrices and carbohydrate standards. The DF contents of plums, atemoyas, and jackfruits obtained by AOAC 2011.25 was higher than those obtained by AOAC 991.43 due to the presence of fructans. The DF content of mature coconuts obtained by the same methods did not present a significant difference. The AOAC 2011.25 method is recommended for fruits with considerable fructans content because it achieves more accurate values.
[Mh] Termos MeSH primário: Frutas
[Mh] Termos MeSH secundário: Brasil
Carboidratos
Fibras na Dieta
Frutanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Dietary Fiber); 0 (Fructans)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


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[PMID]:28867090
[Au] Autor:Judprasong K; Archeepsudcharit N; Chantapiriyapoon K; Tanaviyutpakdee P; Temviriyanukul P
[Ad] Endereço:Institute of Nutrition, Mahidol University, Putthamonthon 4 Rd., Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand. Electronic address: Kunchit.jud@mahidol.ac.th.
[Ti] Título:Nutrients and natural toxic substances in commonly consumed Jerusalem artichoke (Helianthus tuberosus L.) tuber.
[So] Source:Food Chem;238:173-179, 2018 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes-Kaentawan in the Thai language-grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard methods. All Kaentawan samples contained considerable amounts of fructans and dietary fiber (15.4±0.2gand3.2±0.8g/100gfresh weight [FW], respectively), as well as potassium and iron (339±61and0.32±0.05mg/100gFW, respectively). All samples had very low amounts of insecticide residues (37 compounds), cyanide, and trypsin inhibitor, as well as Pb, Cd, nitrate, and nitrite (0.82±0.09, 0.10±0.02, 1.9-17.5, and 0.01-0.24mgkg FW, respectively), in addition to oxalate and phytate (14±9and0.17±0.02mg/100gFW, respectively). This study's data can be used for food composition databases and for safety consumption.
[Mh] Termos MeSH primário: Helianthus
[Mh] Termos MeSH secundário: Alimentos
Frutanos
Tubérculos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fructans)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


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[PMID]:28954640
[Au] Autor:Clarke ST; Brooks SPJ; Inglis GD; Yanke LJ; Green J; Petronella N; Ramdath DD; Bercik P; Green-Johnson JM; Kalmokoff M
[Ad] Endereço:1Applied Bioscience Graduate Program, Faculty of Science,University of Ontario Institute of Technology,Oshawa,ON,Canada,L1H 7K4.
[Ti] Título:Impact of ß2-1 fructan on faecal community change: results from a placebo-controlled, randomised, double-blinded, cross-over study in healthy adults.
[So] Source:Br J Nutr;118(6):441-453, 2017 Sep.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Healthy adults (n 30) participated in a placebo-controlled, randomised, double-blinded, cross-over study consisting of two 28 d treatments (ß2-1 fructan or maltodextrin; 3×5 g/d) separated by a 14-d washout. Subjects provided 1 d faecal collections at days 0 and 28 of each treatment. The ability of faecal bacteria to metabolise ß2-1 fructan was common; eighty-seven species (thirty genera, and four phyla) were isolated using anaerobic medium containing ß2-1 fructan as the sole carbohydrate source. ß2-1 fructan altered the faecal community as determined through analysis of terminal restriction fragment length polymorphisms and 16S rRNA genes. Supplementation with ß2-1 fructan reduced faecal community richness, and two patterns of community change were observed. In most subjects, ß2-1 fructan reduced the content of phylotypes aligning within the Bacteroides, whereas increasing those aligning within bifidobacteria, Faecalibacterium and the family Lachnospiraceae. In the remaining subjects, supplementation increased the abundance of Bacteroidetes and to a lesser extent bifidobacteria, accompanied by decreases within the Faecalibacterium and family Lachnospiraceae. ß2-1 Fructan had no impact on the metagenome or glycoside hydrolase profiles in faeces from four subjects. Few relationships were found between the faecal bacterial community and various host parameters; Bacteroidetes content correlated with faecal propionate, subjects whose faecal community contained higher Bacteroidetes produced more caproic acid independent of treatment, and subjects having lower faecal Bacteroidetes exhibited increased concentrations of serum lipopolysaccharide and lipopolysaccharide binding protein independent of treatment. We found no evidence to support a defined health benefit for the use of ß2-1 fructans in healthy subjects.
[Mh] Termos MeSH primário: Bacteroidetes/metabolismo
Bifidobacterium/metabolismo
Fezes/microbiologia
Frutanos/administração & dosagem
[Mh] Termos MeSH secundário: Adolescente
Adulto
Bacteroidetes/isolamento & purificação
Bifidobacterium/isolamento & purificação
Estudos Cross-Over
Suplementos Nutricionais
Método Duplo-Cego
Feminino
Seres Humanos
Masculino
Metagenoma
Meia-Idade
Polimorfismo de Fragmento de Restrição
Polissacarídeos/administração & dosagem
RNA Ribossômico 16S/isolamento & purificação
Análise de Sequência de DNA
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Fructans); 0 (Polysaccharides); 0 (RNA, Ribosomal, 16S); 7CVR7L4A2D (maltodextrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002318


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[PMID]:28764075
[Au] Autor:Moreno-Vilet L; Bostyn S; Flores-Montaño JL; Camacho-Ruiz RM
[Ad] Endereço:CONACYT- Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Guadalajara, Jalisco, Mexico.
[Ti] Título:Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans.
[So] Source:Food Chem;237:833-840, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7°C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36mL/min. The exclusion range is from 1 to 49 of polymerization degree (180-7966Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity.
[Mh] Termos MeSH primário: Agave
Cromatografia Líquida de Alta Pressão
[Mh] Termos MeSH secundário: Cromatografia em Gel
Frutanos
Peso Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fructans)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28552955
[Au] Autor:Yáñez A; Tapia G; Guerra F; Del Pozo A
[Ad] Endereço:Centro de Mejoramiento Genético y Fenómica Vegetal, Facultad de Ciencias Agrarias, PIEI Adaptación de la Agricultura al Cambio Climático (A2C2), Universidad de Talca, Talca, Chile.
[Ti] Título:Stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (Triticum aestivum L.) genotypes with contrasting tolerance to water stress.
[So] Source:PLoS One;12(5):e0177667, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genetic and physiological mechanisms underlying the relationship between water-soluble carbohydrates (WSC) and water stress tolerance are scarcely known. This study aimed to evaluate the main WSC in stems, and the expression of genes involved in fructan metabolism in wheat genotypes growing in a glasshouse with water stress (WS; 50% field capacity from heading) and full irrigation (FI; 100% field capacity). Eight wheat genotypes (five tolerant and three susceptible to water stress) were evaluated initially (experiment 1) and the two most contrasting genotypes in terms of WSC accumulation were evaluated in a subsequent experiment (experiment 2). Maximum accumulation of WSC occurred 10-20 days after anthesis. Under WS, the stress-tolerant genotype exhibited higher concentrations of WSC, glucose, fructose and fructan in the stems, compared to FI. In addition, the stress-tolerant genotype exhibited higher up-regulation of the fructan 1-fructosyltransferase B (1-FFTB) and fructan 1-exohydrolase w2 (1-FEHw2) genes, whereas the susceptible cultivar presented an up-regulation of the fructan 6-fructosyltransferase (6-SFT) and fructan 1-exohydrolase w3 (1-FEHw3) genes. Our results indicated clear differences in the pattern of WSC accumulation and the expression of genes regulating fructan metabolism between the tolerant and susceptible genotypes under WS.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Secas
Frutanos/metabolismo
Genes de Plantas
Caules de Planta/metabolismo
Triticum/metabolismo
[Mh] Termos MeSH secundário: Triticum/crescimento & desenvolvimento
Triticum/fisiologia
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fructans); 059QF0KO0R (Water)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177667


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[PMID]:28421913
[Au] Autor:Tian Y; Zhao Y; Shi L; Cui Z; Hu B; Zhao Y
[Ad] Endereço:First, third, and fifth authors: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China; second author: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences
[Ti] Título:Type VI Secretion Systems of Erwinia amylovora Contribute to Bacterial Competition, Virulence, and Exopolysaccharide Production.
[So] Source:Phytopathology;107(6):654-661, 2017 06.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The type VI secretion system (T6SS) plays a major role in mediating interbacterial competition and might contribute to virulence in plant pathogenic bacteria. However, the role of T6SS in Erwinia amylovora remains unknown. In this study, 33 deletion mutants within three T6SS clusters were generated in E. amylovora strain NCPPB1665. Our results showed that all 33 mutants displayed reduced antibacterial activities against Escherichia coli as compared with that of the wild-type (WT) strain, indicating that Erwinia amylovora T6SS are functional. Of the 33 mutants, 19 exhibited reduced virulence on immature pear fruit as compared with that of the WT strain. Among them, 6, 1, and 12 genes belonged to T6SS-1, T6SS-2, and T6SS-3 clusters, respectively. Interestingly, these 19 mutants also produced less amylovoran or levan or both. These findings suggest that E. amylovora T6SS play a role in bacterial competition and virulence possibly by influencing exopolysaccharide production.
[Mh] Termos MeSH primário: Erwinia amylovora
Frutanos/metabolismo
Doenças das Plantas/microbiologia
Polissacarídeos Bacterianos/metabolismo
Pyrus/microbiologia
Sistemas de Secreção Tipo VI/metabolismo
[Mh] Termos MeSH secundário: Erwinia amylovora/genética
Erwinia amylovora/patogenicidade
Erwinia amylovora/fisiologia
Frutas/microbiologia
Deleção de Sequência
Sistemas de Secreção Tipo VI/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fructans); 0 (Polysaccharides, Bacterial); 0 (Type VI Secretion Systems); 0 (amylovoran); 9013-95-0 (levan)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-11-16-0393-R


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[PMID]:28400025
[Au] Autor:Ua-Arak T; Jakob F; Vogel RF
[Ad] Endereço:Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
[Ti] Título:Influence of levan-producing acetic acid bacteria on buckwheat-sourdough breads.
[So] Source:Food Microbiol;65:95-104, 2017 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Buckwheat sourdoughs supplemented with molasses as natural sucrose source were fermented with levan-producing Gluconobacter (G.) albidus TMW 2.1191 and Kozakia (K.) baliensis NBRC 16680. Cell growth, concomitant levan and low-molecular-weight metabolite production were monitored. Sourdough breads were prepared with different sourdoughs from both strains (24, 30 and 48 h fermentation, respectively) and analyzed with respect to bread volume, crumb hardness and sensory characteristics. During fermentation, levan, acetic and gluconic acids were increasingly produced, while spontaneously co-growing lactic acid bacteria additionally formed acetic and lactic acids. Sourdoughs from both strains obtained upon 24 h of fermentation significantly improved the bread sensory and quality, including higher specific volume as well as lower crumb hardness. Buckwheat doughs containing isolated levan, with similar molecular size and mass compared to in situ produced levan in the sourdough at 48 h, verified the positive effect of levan on bread quality. However, the positive effects of levan were masked to a certain extent by the impact from the natural acidification during fermentations. While levan-producing acetic acid bacteria are a promising alternative for the development of clean-label gluten-free breads without the need of additives, an appropriate balance between acidification and levan production (amount and structure) must be reached.
[Mh] Termos MeSH primário: Ácido Acético/metabolismo
Acetobacteraceae/metabolismo
Pão/microbiologia
Fagopyrum/microbiologia
Frutanos/biossíntese
Gluconobacter/metabolismo
[Mh] Termos MeSH secundário: Acetobacteraceae/crescimento & desenvolvimento
Antineoplásicos
Bactérias/metabolismo
Pão/análise
Fermentação
Farinha/microbiologia
Microbiologia de Alimentos
Frutanos/metabolismo
Gluconobacter/crescimento & desenvolvimento
Glutens
Lactobacillaceae/crescimento & desenvolvimento
Lactobacillaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Fructans); 8002-80-0 (Glutens); 9013-95-0 (levan); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170427
[Lr] Data última revisão:
170427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


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[PMID]:28367622
[Au] Autor:Struyf N; Laurent J; Verspreet J; Verstrepen KJ; Courtin CM
[Ad] Endereço:Laboratory of Food Chemistry and Biochemistry, Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven , Kasteelpark Arenberg 20, B-3001 Leuven, Belgium.
[Ti] Título:Substrate-Limited Saccharomyces cerevisiae Yeast Strains Allow Control of Fermentation during Bread Making.
[So] Source:J Agric Food Chem;65(16):3368-3377, 2017 Apr 26.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Identification and use of yeast strains that are unable to consume one or more otherwise fermentable substrate types could allow a more controlled fermentation process with more flexibility regarding fermentation times. In this study, Saccharomyces cerevisiae strains with different capacities to consume substrates present in wheat were selected to investigate the impact of substrate limitation on dough fermentation and final bread volume. Results show that fermentation of dough with maltose-negative strains relies on the presence of fructan and sucrose as fermentable substrates and can be used for regular bread making. Levels of fructan and sucrose, endogenously present or added, hence determine the extent of fermentation and timing at the proofing stage. Whole meal is inherently more suitable for substrate-limited fermentation than white flour due to the presence of higher native levels of these substrates. Bread making protocols with long fermentation times are accommodated by addition of substrates such as sucrose.
[Mh] Termos MeSH primário: Pão/microbiologia
Saccharomyces cerevisiae/metabolismo
Triticum/microbiologia
[Mh] Termos MeSH secundário: Pão/análise
Fermentação
Farinha/análise
Farinha/microbiologia
Frutanos/metabolismo
Maltose/metabolismo
Sacarose/metabolismo
Fatores de Tempo
Triticum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fructans); 57-50-1 (Sucrose); 69-79-4 (Maltose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170612
[Lr] Data última revisão:
170612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00313


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[PMID]:28293641
[Au] Autor:Rivera-Huerta M; Lizárraga-Grimes VL; Castro-Torres IG; Tinoco-Méndez M; Macías-Rosales L; Sánchez-Bartéz F; Tapia-Pérez GG; Romero-Romero L; Gracia-Mora MI
[Ad] Endereço:Unidad de Investigación Preclínica (UNIPREC), Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
[Ti] Título:Functional Effects of Prebiotic Fructans in Colon Cancer and Calcium Metabolism in Animal Models.
[So] Source:Biomed Res Int;2017:9758982, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inulin-type fructans are polymers of fructose molecules and are known for their capacity to enhance absorption of calcium and magnesium, to modulate gut microbiota and energy metabolism, and to improve glycemia. We evaluated and compared the effects of Chicory inulin "Synergy 1®" and inulin from Mexican agave "Metlin®" in two experimental models of colon cancer and bone calcium metabolism in mice and rats. Inulins inhibited the development of dextran sulfate sodium-induced colitis and colon cancer in mice; these fructans reduced the concentration of tumor necrosis factor alpha and prevented the formation of intestinal polyps, villous atrophy, and lymphoid hyperplasia. On the other hand, inulin treatments significantly increased bone densitometry (femur and vertebra) in ovariectomized rats without altering the concentration of many serum biochemical parameters and urinary parameters. Histopathology results were compared between different experimental groups. There were no apparent histological changes in rats treated with inulins and a mixture of inulins-isoflavones. Our results showed that inulin-type fructans have health-promoting properties related to enhanced calcium absorption, potential anticancer properties, and anti-inflammatory effects. The use of inulin as a prebiotic can improve health and prevent development of chronic diseases such as cancer and osteoporosis.
[Mh] Termos MeSH primário: Cálcio na Dieta/metabolismo
Neoplasias do Colo/tratamento farmacológico
Frutanos/química
Prebióticos
[Mh] Termos MeSH secundário: Agave
Animais
Osso e Ossos/efeitos dos fármacos
Densitometria
Suplementos Nutricionais
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Feminino
Absorção Intestinal
Inulina/química
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Fitoterapia
Extratos Vegetais/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium, Dietary); 0 (Fructans); 0 (Plant Extracts); 0 (Prebiotics); 9005-80-5 (Inulin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1155/2017/9758982


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[PMID]:28274423
[Au] Autor:Miranda-Molina A; Castillo E; Lopez Munguia A
[Ad] Endereço:Departamento Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, C.P. 62209 Cuernavaca-Mor., Mexico.
[Ti] Título:A novel two-step enzymatic synthesis of blastose, a ß-d-fructofuranosyl-(2↔6)-d-glucopyranose sucrose analogue.
[So] Source:Food Chem;227:202-210, 2017 Jul 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Blastose, a natural disaccharide found in honey, is usually found as a byproduct of fructo-oligosaccharide synthesis from sucrose with fructosyltransferases. In this study, we describe a novel two-step biosynthetic route to obtain blastose, designed from a detailed observation of B. subtilis levansucrase (SacB) acceptor structural requirements for fructosylation. The strategy consisted first in the synthesis of the trisaccharide O-ß-d-Fruf-(2↔6)-O-α-d-Glcp-(1↔1)-α-d-Glcp, through a regioselective ß-d-transfructosylation of trehalose (Tre) which acts as acceptor in a reaction catalyzed by SacB using sucrose or levan as fructosyl donor. In this reaction, levansucrase (LS) transfers regioselectively a fructosyl residue to either C -OH group of the glucose residues in Tre. The resulting trisaccharide obtained in 23% molar yield based on trehalose, was purified and fully characterized by extensive NMR studies. In the second step, the trisaccharide is specifically hydrolyzed by trehalase, to obtain blastose in 43.2% molar yield based on the trisaccharide. This is the first report describing the formation of blastose through a sequential transfuctosylation-hydrolysis reaction.
[Mh] Termos MeSH primário: Dissacaridases/metabolismo
Hexosiltransferases/metabolismo
Trealose/metabolismo
Trissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Bacillus subtilis/enzimologia
Proteínas de Bactérias/metabolismo
Frutanos/metabolismo
Hidrólise
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fructans); 0 (Trisaccharides); 0 (blastose); 57-50-1 (Sucrose); 9013-95-0 (levan); B8WCK70T7I (Trehalose); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.10 (levansucrase); EC 3.2.1.- (Disaccharidases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE



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