Base de dados : MEDLINE
Pesquisa : D09.698.357 [Categoria DeCS]
Referências encontradas : 382 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 39 ir para página                         

  1 / 382 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28461332
[Au] Autor:Temple MJ; Cuskin F; Baslé A; Hickey N; Speciale G; Williams SJ; Gilbert HJ; Lowe EC
[Ad] Endereço:From the Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE4 2HH, United Kingdom and.
[Ti] Título:A Bacteroidetes locus dedicated to fungal 1,6-ß-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-ß-glucanase.
[So] Source:J Biol Chem;292(25):10639-10650, 2017 06 23.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycans are major nutrients available to the human gut microbiota. The are generalist glycan degraders, and this function is mediated largely by polysaccharide utilization loci (PULs). The genomes of several species contain a PUL, PUL , that was predicted to target mixed linked plant 1,3;1,4-ß-glucans. To test this hypothesis we characterized the proteins encoded by this locus in , a member of the human gut microbiota. We show here that PUL does not orchestrate the degradation of a plant polysaccharide but targets a fungal cell wall glycan, 1,6-ß-glucan, which is a growth substrate for the bacterium. The locus is up-regulated by 1,6-ß-glucan and encodes two enzymes, a surface endo-1,6-ß-glucanase, BT3312, and a periplasmic ß-glucosidase that targets primarily 1,6-ß-glucans. The non-catalytic proteins encoded by PUL target 1,6-ß-glucans and comprise a surface glycan-binding protein and a SusD homologue that delivers glycans to the outer membrane transporter. We identified the central role of the endo-1,6-ß-glucanase in 1,6-ß-glucan depolymerization by deleting , which prevented the growth of on 1,6-ß-glucan. The crystal structure of BT3312 in complex with ß-glucosyl-1,6-deoxynojirimycin revealed a TIM barrel catalytic domain that contains a deep substrate-binding cleft tailored to accommodate the hook-like structure adopted by 1,6-ß-glucan. Specificity is driven by the complementarity of the enzyme active site cleft and the conformation of the substrate. We also noted that PUL is syntenic to many PULs from other Bacteroidetes, suggesting that utilization of yeast and fungal cell wall 1,6-ß-glucans is a widespread adaptation within the human microbiota.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Bacteroidetes/enzimologia
Polissacarídeos Fúngicos/química
Glicosídeo Hidrolases/química
beta-Glucanas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Bacteroidetes/genética
Configuração de Carboidratos
Cristalografia por Raios X
Loci Gênicos
Glicosídeo Hidrolases/genética
Seres Humanos
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fungal Polysaccharides); 0 (beta-Glucans); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.75 (endo-1,6-beta-glucanase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.787606


  2 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28566667
[Au] Autor:Tsunemi Y
[Ad] Endereço:Department of Dermatology, Tokyo Women's Medical University.
[Ti] Título:Dermatophyte Antigen Kit.
[So] Source:Med Mycol J;58(2):J51-J54, 2017.
[Is] ISSN:1882-0476
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:The dermatophyte antigen kit uses monoclonal antibodies that react with polysaccharides present in the dermatophyte cell wall to detect dermatophyte antigens in specimens based on the principle of immunochromatography. Clinical studies showed that the kit was very useful in the diagnosis of tinea unguium but not tinea pedis. The kit was therefore further developed as an in vitro diagnostic tool for tinea unguium and was approved by the Pharmaceuticals and Medical Devices Agency of Japan. The kit's extraction solution can extract antigens from nail specimens quickly and efficiently. When direct microscopy fails to detect fungal elements in a specimen of suspected tinea unguium, the kit can be used so that positive samples are re-examined by direct microscopy, in order to reduce the likelihood of false-negative detection. In addition, in settings where direct microscopy is unavailable, the kit can be used so that treatment for dermatophytes is withheld when results are negative. Such an approach can reduce both wasteful treatment and medical costs. It is important to note that the kit is used to complement conventional fungus testing methods and that direct microscopy must be used to confirm the final morphological diagnosis of the pathogenic fungal infection. Use of a combination of direct microscopy and this kit should improve the accuracy of diagnosis of tinea unguium.
[Mh] Termos MeSH primário: Arthrodermataceae/imunologia
Onicomicose/diagnóstico
Kit de Reagentes para Diagnóstico
[Mh] Termos MeSH secundário: Anticorpos Monoclonais
Antígenos de Fungos/imunologia
Antígenos de Fungos/isolamento & purificação
Arthrodermataceae/patogenicidade
Arthrodermataceae/ultraestrutura
Reações Falso-Negativas
Polissacarídeos Fúngicos/imunologia
Seres Humanos
Imunocromatografia
Microscopia
Onicomicose/diagnóstico por imagem
Onicomicose/microbiologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens, Fungal); 0 (Fungal Polysaccharides); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171015
[Lr] Data última revisão:
171015
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.3314/mmj.17.005


  3 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28257189
[Au] Autor:Span EA; Suess DLM; Deller MC; Britt RD; Marletta MA
[Ad] Endereço:Biophysics Graduate Group, University of California, Berkeley , Berkeley, California 94720, United States.
[Ti] Título:The Role of the Secondary Coordination Sphere in a Fungal Polysaccharide Monooxygenase.
[So] Source:ACS Chem Biol;12(4):1095-1103, 2017 Apr 21.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polysaccharide monooxygenases (PMOs) are secreted metalloenzymes that catalyze the oxidative degradation of polysaccharides in a copper-, oxygen-, and reductant-dependent manner. Cellulose-active fungal PMOs degrade cellulosic substrates to be utilized as a carbon source for fungal growth. To gain insight into the PMO mechanism, the role of conserved residues in the copper coordination sphere was investigated. Here, we report active-site hydrogen-bonding motifs in the secondary copper coordination sphere of MtPMO3*, a C1-oxidizing PMO from the ascomycete fungus Myceliophthora thermophila. A series of point substitutions that disrupt this conserved network are used to interrogate its function. Activity assays, in conjunction with EPR spectroscopy, demonstrate that residues H161 and Q167 are involved in stabilizing bound oxygen, and H161 appears to play a role in proton transfer. Additionally, Q167 increases the ligand donor strength of Y169 to the copper via a hydrogen-bonding interaction. Altogether, H161 and Q167 are important for oxygen activation, and the results are suggestive of a copper-oxyl active intermediate.
[Mh] Termos MeSH primário: Polissacarídeos Fúngicos/química
Oxigenases de Função Mista/química
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Ligações de Hidrogênio
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Polysaccharides); EC 1.- (Mixed Function Oxygenases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00016


  4 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28167111
[Au] Autor:Jiao F; Wang X; Song X; Jing H; Li S; Ren Z; Gao Z; Zhang J; Jia L
[Ad] Endereço:College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China; School of Public Health, Taishan Medical University, Taian, Shandong 271016, PR China.
[Ti] Título:Processing optimization and anti-oxidative activity of enzymatic extractable polysaccharides from Pleurotus djamor.
[So] Source:Int J Biol Macromol;98:469-478, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In present study, a quadratic regression model based on the response surface methodology (RSM) coupled with Box-Behnken design (BBD) was employed to optimize the enzyme-assisted extraction (EAE) process of enzymatic-extractable Pleurotus djamor polysaccharides (EnPPs). By solving the regression equations and analyzing the model graphs, the optimum conditions were at pH 7.36, water to material ratio 56.78, extraction time 44.77min and extraction temperature 35.36°C, respectively. Under these conditions, the experimental yields of EnPPs reached 3.61%, which were in good agreement with the validated values (3.57±0.51%). Basic physicochemical properties including molecular weights, structural identification by FT-IR and monosaccharide compositions were processed. In addition, the in vivo inhibiting activities against oxidative stress were investigated. The results provided an alternative bioresource for the exploitation of EnPPs under EAE from P. djamor, and the EnPPs had potential effects in prevention of oxidative stress.
[Mh] Termos MeSH primário: Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Enzimas/metabolismo
Polissacarídeos Fúngicos/isolamento & purificação
Polissacarídeos Fúngicos/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Pleurotus/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Polissacarídeos Fúngicos/química
Concentração de Íons de Hidrogênio
Modelos Teóricos
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Enzymes); 0 (Fungal Polysaccharides)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


  5 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28165422
[Au] Autor:Liu Q; Zhu M; Geng X; Wang H; Ng TB
[Ad] Endereço:Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. liuqin_bio@hotmail.com.
[Ti] Título:Characterization of Polysaccharides with Antioxidant and Hepatoprotective Activities from the Edible Mushroom Oudemansiella radicata.
[So] Source:Molecules;22(2), 2017 Feb 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The preliminary structure, in vitro antioxidant and in vivo hepatoprotective activities of water-soluble polysaccharides (ORWP) and alkali-soluble polysaccharides (ORAP), prepared from the mushroom , were investigated. Both ORWP and ORAP were heteropolysaccharides with mannose, glucose and galactose being the main monosaccharide components. Regarding the antioxidant activities, ORWP and ORAP showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide scavenging activity and lipid peroxidation inhibitory effects, as well as moderate reducing power and Fe chelating activity. For the hepatoprotective activity, administration of ORWP and ORAP prevented the increase in serum alanine aminotransferase and aspartate aminotransferase activities in a carbon tetrachloride-induced acute liver damage model, suppressed hepatic malondialdehyde formation and stimulated the activities of hepatic superoxide dismutase and glutathione peroxidase. Thus, we speculate that ORWP and ORAP may protect the liver from CCl4-induced hepatic damage via antioxidant mechanisms.
[Mh] Termos MeSH primário: Agaricales/química
Antioxidantes/química
Antioxidantes/farmacologia
Polissacarídeos Fúngicos/química
Polissacarídeos Fúngicos/farmacologia
Hepatócitos/efeitos dos fármacos
Hepatócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes/isolamento & purificação
Radicais Livres/antagonistas & inibidores
Polissacarídeos Fúngicos/isolamento & purificação
Quelantes de Ferro/química
Quelantes de Ferro/farmacologia
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/metabolismo
Testes de Função Hepática
Camundongos
Oxirredução/efeitos dos fármacos
Substâncias Protetoras/química
Substâncias Protetoras/isolamento & purificação
Substâncias Protetoras/farmacologia
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Free Radicals); 0 (Fungal Polysaccharides); 0 (Iron Chelating Agents); 0 (Protective Agents)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE


  6 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28108410
[Au] Autor:Zhang YS; Li WJ; Zhang XY; Yan YX; Nie SP; Gong DM; Tang XF; He M; Xie MY
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
[Ti] Título:Ganoderma atrum polysaccharide ameliorates anoxia/reoxygenation-mediated oxidative stress and apoptosis in human umbilical vein endothelial cells.
[So] Source:Int J Biol Macromol;98:398-406, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ganoderma atrum polysaccharide (PSG-1), a main polysaccharide from Ganoderma atrum, possesses potent antioxidant capacity and cardiovascular benefits. The aim of this study was to investigate the role of PSG-1 in oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) under anoxia/reoxygenation (A/R) injury conditions. The results showed that exposure of HUVECs to A/R triggered cell death and apoptosis. Administration of PSG-1 significantly inhibited A/R-induced cell death and apoptosis in HUVECs. PSG-1-reduced A/R injury was mediated via mitochondrial apoptotic pathway, as evidenced by elevation of mitochondrial Bcl-2 protein and mitochondrial membrane potential, and attenuation of Bax translocation, cytochrome c release and caspases activation. Furthermore, PSG-1 enhanced the activities of superoxide dismutase, catalase and glutathione peroxidase and glutathione content, and concomitantly attenuated reactive oxygen species generation, lipid peroxidation and glutathione disulfide content. The antioxidant, N-acetyl-l-cysteine, significantly ameliorated all of these endothelial injuries caused by A/R, suggesting that antioxidant activities might play a key role in PSG-1-induced endothelial protection. Taken together, these findings suggested that PSG-1 could be as a promising adjuvant against endothelial dysfunction through ameliorating oxidative stress and apoptosis.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Polissacarídeos Fúngicos/farmacologia
Ganoderma/química
Células Endoteliais da Veia Umbilical Humana/citologia
Células Endoteliais da Veia Umbilical Humana/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Caspases/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Citocromos c/secreção
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Seres Humanos
Peroxidação de Lipídeos/efeitos dos fármacos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fungal Polysaccharides); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Reactive Oxygen Species); 0 (bcl-2-Associated X Protein); 9007-43-6 (Cytochromes c); EC 3.4.22.- (Caspases); S88TT14065 (Oxygen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE


  7 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28105862
[Au] Autor:Carrieri R; Manco R; Sapio D; Iannaccone M; Fulgione A; Papaianni M; de Falco B; Grauso L; Tarantino P; Ianniello F; Lanzotti V; Lahoz E; Capparelli R
[Ad] Endereço:a Dipartimento di Colture Industriali , Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA) , Caserta , Italy.
[Ti] Título:Structural data and immunomodulatory properties of a water-soluble heteroglycan extracted from the mycelium of an Italian isolate of Ganoderma lucidum.
[So] Source:Nat Prod Res;31(18):2119-2125, 2017 Sep.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mushrooms produce a wide range of bioactive polysaccharides, different from each other in chemical structure and biological effects. In the last years, the idea to develop functional foods or drugs containing fungal polysaccharides is attracting great attention. Fruiting bodies of Basidiomycetes Ganoderma lucidum are commonly used in Oriental medicine to treat several disorders. G. lucidum polysaccharides - mainly ß-glucans and heteroglycans - have numerous biological properties such as antitumour and immunomodulatory activities. This report shows, by gene expression analyses and bioenergetic assays, immunomodulatory properties and capacity to improve glucose metabolism of a water-soluble heteroglycan extracted from mycelium of an Italian isolate of G. lucidum. The findings suggest the use of the heteroglycan as probiotic or ingredient in functional foods, being easy to produce and disperse in a food matrix thanks to its water-solubility. Heteroglycan could exert protective effects in pro-inflammatory conditions and benefits for people characterised by suppressed immune response.
[Mh] Termos MeSH primário: Polissacarídeos Fúngicos/química
Polissacarídeos Fúngicos/farmacologia
Glucanos/farmacologia
Fatores Imunológicos/farmacologia
Reishi/química
[Mh] Termos MeSH secundário: Linhagem Celular
Citocinas/genética
Avaliação Pré-Clínica de Medicamentos/métodos
Regulação da Expressão Gênica/efeitos dos fármacos
Glucanos/química
Glucose/metabolismo
Seres Humanos
Fatores Imunológicos/química
Itália
Espectroscopia de Ressonância Magnética
Medicina Tradicional do Leste Asiático
Micélio/química
Solubilidade
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Fungal Polysaccharides); 0 (Glucans); 0 (Immunologic Factors); 059QF0KO0R (Water); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1278593


  8 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28089931
[Au] Autor:Liu F; Wang Y; Zhang K; Wang Y; Zhou R; Zeng Y; Han Y; Ng TB
[Ad] Endereço:The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
[Ti] Título:A novel polysaccharide with antioxidant, HIV protease inhibiting and HIV integrase inhibiting activities from Fomitiporia punctata (P. karst.) murrill (Basidiomycota, hymenochaetales).
[So] Source:Int J Biol Macromol;97:339-347, 2017 Apr.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel polysaccharide fraction (G ) was obtained from the fungus Fomitiporia punctata (P. Karst.) Murrill. G exhibited a molecular weight of approximately 151kDa. The FT-IR results suggested that the monosaccharide components of G1 possessed furanoid rings and there were ß-glycosidic bonds between the sugar units. The H NMR results showed that G1 was composed of arabinose, fructose, galactose and glucose in the molar ratio of 1.6:3.8:19.7:19.7, as determined by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). G produced significant antioxidant effects as evidenced by its potency in inhibiting erythrocyte hemolysis, and in scavenging hydroxyl radicals and superoxide radicals. The highest rates of inhibition achieved were 73.58%, 36.55% and 50.98% respectively. In addition, G brought about 19.6% inhibition of HIV-1 protease activity at the concentration of 50µg/mL. G1 displayed inhibitory activity toward HIV-1 integrase in the concentration range of 100-1000µg/mL. The present study indicates that G from Fomitiporia punctate (P. Karst.) Murrill is a novel natural antioxidant.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Basidiomycota/química
Polissacarídeos Fúngicos/farmacologia
Inibidores de Integrase de HIV/farmacologia
Integrase de HIV/metabolismo
Inibidores da Protease de HIV/farmacologia
Protease de HIV/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Polissacarídeos Fúngicos/química
Polissacarídeos Fúngicos/isolamento & purificação
Inibidores de Integrase de HIV/química
Inibidores de Integrase de HIV/isolamento & purificação
Inibidores da Protease de HIV/química
Inibidores da Protease de HIV/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fungal Polysaccharides); 0 (HIV Integrase Inhibitors); 0 (HIV Protease Inhibitors); EC 2.7.7.- (HIV Integrase); EC 3.4.23.- (HIV Protease)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE


  9 / 382 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28069348
[Au] Autor:Li X; Wang L; Wang Z
[Ad] Endereço:School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. Electronic address: lixiaoyu@ysu.edu.cn.
[Ti] Título:Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina.
[So] Source:Int J Biol Macromol;98:59-66, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previous research found that the polysaccharides isolated from Hohenbuehelia serotina possessed various biological activities, such as antioxidant, immunomodulation and radioprotective effects. However, the structural information of H. serotina polysaccharides has not yet been reported. Therefore, based on the investigation of the antioxidative tracking, a novel polysaccharide named as NTHSP-A1 was isolated from H. serotina by ultrasonic-assistance extraction and anion-exchange and gel permeation chromatography approaches. Structural characterization revealed that NTHSP-A1 had an average molecular weight of 8.09×10 Da and composed of arabinose, mannose, glucose and galactose in a molar ratio of 4:16:28:11. The polysaccharide was semi-crystalline substance with multi-branching structure. The backbone of NTHSP-A1 was shown to contain →3,6)-α-d-Glcp-(1→, with branches substituted at C-3 of →2)-α-l-Arap-(1→, C-3 of α-d-Manp-(1→, and C-6 of →6)-ß-d-Galp-(1→, respectively. This study provides a theoretical basis for the further research on the relationship between biological activity and structure of NTHSP-A1.
[Mh] Termos MeSH primário: Agaricales/química
Antioxidantes/química
Antioxidantes/farmacologia
Polissacarídeos Fúngicos/química
Polissacarídeos Fúngicos/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/isolamento & purificação
Sequência de Carboidratos
Polissacarídeos Fúngicos/isolamento & purificação
Metilação
Peso Molecular
Monossacarídeos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fungal Polysaccharides); 0 (Monosaccharides)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


  10 / 382 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28031206
[Au] Autor:Grossman NT; Casadevall A
[Ad] Endereço:Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
[Ti] Título:Physiological Differences in Cryptococcus neoformans Strains versus and Their Effects on Antifungal Susceptibility.
[So] Source:Antimicrob Agents Chemother;61(3), 2017 Mar.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:is an environmentally ubiquitous fungal pathogen that primarily causes disease in people with compromised immune systems, particularly those with advanced AIDS. There are estimated to be almost 1 million cases per year of cryptococcal meningitis in patients infected with human immunodeficiency virus, leading to over 600,000 annual deaths, with a particular burden in sub-Saharan Africa. Amphotericin B (AMB) and fluconazole (FLC) are key components of cryptococcal meningitis treatment: AMB is used for induction, and FLC is for consolidation, maintenance and, for occasional individuals, prophylaxis. However, the results of standard antifungal susceptibility testing (AFST) for AMB and FLC do not correlate well with therapeutic outcomes and, consequently, no clinical breakpoints have been established. While a number of explanations for this absence of correlation have been proffered, one potential reason that has not been adequately explored is the possibility that the physiological differences between the infection environment and the AFST environment lead to disparate drug susceptibilities. These susceptibility-influencing factors include melanization, which does not occur during AFST, the size of the polysaccharide capsule, which is larger in infecting cells than in those grown under normal laboratory conditions, and the presence of large polyploid "titan cells," which rarely occur under laboratory conditions. Understanding whether and how differentially expresses mechanisms of resistance to AMB and FLC in the AFST environment compared to the environment could enhance our ability to interpret AFST results and possibly lead to the development of more applicable testing methods.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Cryptococcus neoformans/efeitos dos fármacos
Cápsulas Fúngicas/efeitos dos fármacos
Proteínas Fúngicas/genética
Regulação Fúngica da Expressão Gênica
Melaninas/biossíntese
[Mh] Termos MeSH secundário: Anfotericina B/farmacologia
Cryptococcus neoformans/patogenicidade
Cryptococcus neoformans/fisiologia
Farmacorresistência Fúngica/fisiologia
Fluconazol/farmacologia
Cápsulas Fúngicas/fisiologia
Polissacarídeos Fúngicos/biossíntese
Proteínas Fúngicas/biossíntese
Infecções por HIV/complicações
Infecções por HIV/microbiologia
Infecções por HIV/patologia
Infecções por HIV/virologia
Seres Humanos
Melaninas/genética
Meningite Criptocócica/complicações
Meningite Criptocócica/microbiologia
Meningite Criptocócica/patologia
Meningite Criptocócica/virologia
Testes de Sensibilidade Microbiana
Pigmentação/fisiologia
Poliploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fungal Polysaccharides); 0 (Fungal Proteins); 0 (Melanins); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161230
[St] Status:MEDLINE



página 1 de 39 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde