Base de dados : MEDLINE
Pesquisa : D09.698.360 [Categoria DeCS]
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[PMID]:29446584
[Au] Autor:Sosedova LM; Novikov MA; Titov EA; Rukavishnikov VS
[Ti] Título:[Induction of apoptosis in neurons of white rats under exposure of nanobiocomposite based on ag (0) nanoparticles and arabinogalactan].
[So] Source:Gig Sanit;95(12):1210-13, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:There are presented results of the immunohistochemical study of neural tissue of outbred albino rats exposed for 9 days to the influence of the silver nanobiocomposite consisted of silver nanoparticles encapsulated into a matrix of a natural polymer - arabinogalactan. The research of albino rats was performed in 2 stages: half of the rats in each groups were decapitated immediately after the exposure (early period) and the rest animals - 6 months after the end of exposure (remote period). The impact of the studied substance was proved to cause functional changes in cells of the nervous tissue. After the subacute administration of the nanobiocomposite - argentum-arabinogalactan (nano-Ag-AG) in cells of the nervous tissue of the brain of albino rats the expression of apoptotic and anti-apoptotic protein (caspase-3 and bcl-2) was established to be changed. The number of normal neurons producing protein caspase-3 sharply increases. Herewith the number of immunonegative neurons fairly declines. Along with this there is noted the high level of bcl-2 content, one function ofwhich is the preclusion ofapoptosis. In preparations there is revealed a significant gain in the number of bcl-2 expressing neurons, however, the protective effect of the protein is not fully realized, that leads to the significantly increase in the content of damaged hyperchromatic cells. The evaluation of results of the immunohistochemical study of the nervous tissue of albino rats according to data concerning the proteins caspase-3 and bcl-2 expression permits to make a conclusion about the capability of nanoargentum encapsulated into polymer matrix by passing the blood-brain barrier to induce the triggering apoptosis cascade in neurons of the cerebral cortex.
[Mh] Termos MeSH primário: Encéfalo
Galactanos/farmacologia
Larix
Nanopartículas Metálicas/efeitos adversos
Prata
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Biopolímeros/farmacologia
Barreira Hematoencefálica/metabolismo
Encéfalo/efeitos dos fármacos
Encéfalo/patologia
Encéfalo/fisiopatologia
Caspase 3/metabolismo
Imuno-Histoquímica
Modelos Animais
Preparações de Plantas/farmacologia
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Ratos
Prata/efeitos adversos
Prata/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biopolymers); 0 (Galactans); 0 (Plant Preparations); 0 (Proto-Oncogene Proteins c-bcl-2); 3M4G523W1G (Silver); EC 3.4.22.- (Caspase 3); SL4SX1O487 (arabinogalactan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


  2 / 2162 MEDLINE  
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[PMID]:28450249
[Au] Autor:Kurt A; Toker OS; Tornuk F
[Ad] Endereço:Department of Food Engineering, Engineering Faculty, Ondokuz Mayis University, 55139, Samsun, Turkey; Department of Food Engineering, Engineering and Architecture Faculty, Bitlis Eren University, 13000 Bitlis, Turkey. Electronic address: abdullahkurt48@gmail.com.
[Ti] Título:Effect of xanthan and locust bean gum synergistic interaction on characteristics of biodegradable edible film.
[So] Source:Int J Biol Macromol;102:1035-1044, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study was aimed to use different combinations of xanthan (XG) and locust bean gum (LBG) in the biodegradable edible film preparation by benefitting from their synergistic interactions for the first time. Concentrations of LBG, XG and glycerol of the optimized film sample were found to be 89.6%, 10.4% and 20%, respectively. At the optimum point the WVP, TS, E% and EM values of film were found 0.22gmmh m kPa, 86.97MPa, 33.34% and 177.25MPa, respectively. The optimized film was characterized for its physical, thermal and structural behavior. The scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FTIR) analyses exhibited miscibility and presence of interaction between polymers. In conclusion, XG and LBG interaction was used successfully to get biodegradable films and coatings with improved characteristics.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Galactanos/química
Mananas/química
Gomas Vegetais/química
Polissacarídeos Bacterianos/química
Embalagem de Produtos
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/metabolismo
Composição de Medicamentos
Glicerol/química
Fenômenos Mecânicos
Permeabilidade
Reologia
Vapor
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides, Bacterial); 0 (Steam); PDC6A3C0OX (Glycerol); TTV12P4NEE (xanthan gum); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  3 / 2162 MEDLINE  
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[PMID]:28873574
[Au] Autor:Owens C; Griffin K; Khouryieh H; Williams K
[Ad] Endereço:Department of Chemistry, Western Kentucky University, Bowling Green, KY, USA.
[Ti] Título:Creaming and oxidative stability of fish oil-in-water emulsions stabilized by whey protein-xanthan-locust bean complexes: Impact of pH.
[So] Source:Food Chem;239:314-322, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impact of pH on the physicochemical properties of 10% menhaden oil-in-water (O/W) emulsions containing 2% whey protein isolate (WPI) and 0.1% xanthan (XG)-locust bean gum (LBG) mixtures was investigated. The O/W emulsions containing 0.1% XG-LBG mixtures were compared to emulsions with 0.1% XG and 0.1% LBG. The results indicated that stability is dependent on pH and biopolymer type. At both pH 3 and 5, emulsions containing either XG or XG-LBG mixtures had large particle sizes, viscosity, droplet aggregation, and creaming index, resulting in poor physical stability which can be related to the adsorbed protein-polysaccharide interactions. At pH7, the XG-LBG emulsions showed the greatest resistance to phase separation and resulted in stable emulsions. Lipid oxidation measurements also indicated that XG-LBG mixtures can be used to form stable emulsions at pH 3 and pH 7. These results have significant implications for the development of novel structures containing lipid phases susceptible to lipid oxidation.
[Mh] Termos MeSH primário: Óleos de Peixe/química
[Mh] Termos MeSH secundário: Animais
Emulsões
Galactanos
Gafanhotos
Concentração de Íons de Hidrogênio
Mananas
Gomas Vegetais
Polissacarídeos Bacterianos
Água
Proteínas do Soro do Leite
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Fish Oils); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides, Bacterial); 0 (Whey Proteins); 059QF0KO0R (Water); TTV12P4NEE (xanthan gum); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  4 / 2162 MEDLINE  
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Bittencourt, Vânia Rita Elias Pinheiro
Texto completo
[PMID]:28655584
[Au] Autor:Tunholi VM; Tunholi-Alves VM; Monteiro CO; Silva LCD; Dolinski CM; Castro RN; Bittencourt VREP; Silva JPD; Freire Martins IV
[Ad] Endereço:Departamento de Medicina Veterinária, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo - UFES, Alegre, ES, Brazil. Electronic address: vinnytunholi@yahoo.com.br.
[Ti] Título:Biological, biochemical and histological features of Bradybaena similaris (Gastropoda: Pulmonata) infected by Heterorabditis indica (Rhabditida: Heterorhabditidae) strain LPP1.
[So] Source:Exp Parasitol;179:28-35, 2017 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the possible biological, biochemical and histological changes in Bradybaena similaris(Gastropoda: Pulmonata) infected by Heterorhabditis indica (Rhabditida: Heterorhabditidae), strain LPP1. Two groups of 16 snails were formed: the control group (unexposed) and the treated group, which was exposed for three weeks to infective juveniles (J ) of H. indica LPP1. The experiment was conducted in duplicate, using a total of 64 snails. After the exposure period, the snails were dissected to collect the hemolymph and tissues, for evaluation of the physiological changes caused by the infection. The number of eggs laid/snail and the viability of these eggs were also assessed as indicators of the reproductive activity of B. similaris. Intense glycogenolysis was accompanied by a significant reduction (p < 0.05) in the glucose content of the hemolymph of the exposed snails, indicating that infection by H. indica induces breakdown of the host's glycemic homeostasis. Significant variations (p < 0.05) in the lactate dehydrogenase activity occurred together with changes in the concentration of pyruvic and lactic acid in the hemolymph of the infected B. similaris snails, corroborating the transition from aerobic to anaerobic metabolism in the hosts. These metabolic alterations reflect the parasitic castration process in this interface. The results suggest that the use of H. indica LPP1 is a potential alternative for biological control of B. similaris.
[Mh] Termos MeSH primário: Gastrópodes/parasitologia
Rhabditoidea/fisiologia
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Cromatografia Líquida de Alta Pressão
Galactanos/análise
Gastrópodes/anatomia & histologia
Gastrópodes/metabolismo
Glucose/metabolismo
Glicogênio/metabolismo
Hemolinfa/química
Hemolinfa/enzimologia
Hemolinfa/metabolismo
L-Lactato Desidrogenase/metabolismo
Ácido Láctico/análise
Mariposas/parasitologia
Ácido Pirúvico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 33X04XA5AT (Lactic Acid); 37208-43-8 (galactogen); 8558G7RUTR (Pyruvic Acid); 9005-79-2 (Glycogen); EC 1.1.1.27 (L-Lactate Dehydrogenase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  5 / 2162 MEDLINE  
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[PMID]:28628733
[Au] Autor:Walczak MA; Zhu F
[Ad] Endereço:Department of Chemistry and Biochemistry, University of Colorado, 215 UCB, Boulder, CO, 80309, USA.
[Ti] Título:Size Matters: Chemical Synthesis of a Homogenous Arabinogalactan 92-mer.
[So] Source:Chembiochem;18(18):1789-1791, 2017 Sep 19.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:31+30+31: Ye and colleagues have synthesized a branched 92-mer of arabinogalactan-a major component of the cell wall of M. tuberculosis-by linking a linear oligogalactan 30-mer with two d-arabinofuranose 31-mers. Their approach capitalizes on sequential, one-pot glycosylation reactions that result in a rapid increase in molecular complexity and efficient synthesis of a branched oligosaccharide.
[Mh] Termos MeSH primário: Galactanos/química
[Mh] Termos MeSH secundário: Parede Celular/metabolismo
Galactanos/síntese química
Mesilatos/química
Morfolinas/química
Mycobacterium tuberculosis/metabolismo
Oligossacarídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 0 (Mesylates); 0 (Morpholines); 0 (Oligosaccharides); 8B2ZCK305O (morpholine); JE2SY203E8 (trifluoromethanesulfonic acid); SL4SX1O487 (arabinogalactan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700311


  6 / 2162 MEDLINE  
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[PMID]:28622474
[Au] Autor:Lim SH
[Ad] Endereço:Department of Biochemistry, School of Medicine, Catholic University of Daegu , Daegu, Korea.
[Ti] Título:Larch Arabinogalactan Attenuates Myocardial Injury by Inhibiting Apoptotic Cascades in a Rat Model of Ischemia-Reperfusion.
[So] Source:J Med Food;20(7):691-699, 2017 Jul.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We reported previously that supplementation with apple pectin, a dietary fiber, reduced myocardial injury in a rat model of ischemia-reperfusion. In this study, we further investigated an arabinogalactan, one of the constituent polysaccharides of pectin, to determine which domains comprising pectin were responsible for the protection. In a rat model of 30-min ischemia followed by 3-h reperfusion, supplementation with larch arabinogalactan (LAG) over 50 mg/kg/day significantly reduced infarct size. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and immunoblot analyses showed that intake of LAG blocked the steps involved in apoptotic cascades through downregulation of gelsolin gene expression at the protein (Gelsolin) level, inhibition of p38 phosphorylation in mitogen-activated protein kinase (MAPK) pathways, decreased bax/bcl-2 ratio at the protein (Bax/Bcl-2) level, which was correlated with the ratio at the mRNA level, inhibition of the conversion of Procaspase protein to Caspase-3 protein, and consequently a decrease in apoptotic cells. In addition, the intake of LAG reduced the hif1-α gene expression at the protein (HIF1-α) level. These findings suggest that arabinogalactan is an active component of pectin for reducing myocardial injury by inhibiting apoptosis in postocclusion steps, possibly indicating that arabinogalactan can be developed as a cardioprotectant to prevent myocardial injury.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Galactanos/administração & dosagem
Larix/química
Isquemia Miocárdica/tratamento farmacológico
Extratos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Caspase 3/genética
Caspase 3/metabolismo
Modelos Animais de Doenças
Seres Humanos
Masculino
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Isquemia Miocárdica/genética
Isquemia Miocárdica/metabolismo
Isquemia Miocárdica/fisiopatologia
Fosforilação
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Ratos
Ratos Sprague-Dawley
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (bcl-2-Associated X Protein); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 3.4.22.- (Caspase 3); SL4SX1O487 (arabinogalactan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.3886


  7 / 2162 MEDLINE  
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[PMID]:28608892
[Au] Autor:Torcello-Gómez A; Foster TJ
[Ad] Endereço:School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, UK. M.A.TorcelloGomez@leeds.ac.uk.
[Ti] Título:Instant polysaccharide-based emulsions: impact of microstructure on lipolysis.
[So] Source:Food Funct;8(6):2231-2242, 2017 Jun 21.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The development of emulsion-based products through optimisation of ingredients, reduction in energy-input during manufacture, while fulfilling healthy attributes, are major objectives within the food industry. Instant emulsions can meet these features, but comprehensive studies are necessary to investigate the effect of the initial formulation on the final microstructure and, in turn, on the in vitro lipolysis, comprising the double aim of this work. The instant emulsion is formed within 1.5-3 min after pouring the aqueous phase into the oil phase which contains a mixture of emulsifier (Tween 20), swelling particles (Sephadex) and thickeners (hydroxypropylmethylcellulose, HPMC, and guar gum, GG) under mild shearing (180 rpm). The creation of oil-in-water emulsions is monitored in situ by viscosity analysis, the final microstructure visualised by microscopy and the release of free fatty acids under simulated intestinal conditions quantified by titration. Increasing the concentration and molecular weight (M ) of GG leads to smaller emulsion droplets due to increased bulk viscosity upon shearing. This droplet size reduction is magnified when increasing the M of HPMC or swelling capacity of viscosifying particles. In addition, in the absence of the emulsifier Tween 20, the sole use of high-Mw HPMC is effective in emulsification due to combined increased bulk viscosity and interfacial activity. Hence, optimisation of the ingredient choice and usage level is possible when designing microstructures. Finally, emulsions with larger droplet size (>20 µm) display a slower rate and lower extent of lipolysis, while finer emulsions (droplet size ≤20 µm) exhibit maximum rate and extent profiles. This correlates with the extent of emulsion destabilisation observed under intestinal conditions.
[Mh] Termos MeSH primário: Emulsões/química
Polissacarídeos/química
[Mh] Termos MeSH secundário: Emulsificantes/química
Galactanos/química
Lipólise
Mananas/química
Peso Molecular
Tamanho da Partícula
Gomas Vegetais/química
Polissorbatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsifying Agents); 0 (Emulsions); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides); 0 (Polysorbates); E89I1637KE (guar gum)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00536a


  8 / 2162 MEDLINE  
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[PMID]:28534922
[Au] Autor:Macho-González A; Garcimartín A; López-Oliva ME; Bertocco G; Naes F; Bastida S; Sánchez-Muniz FJ; Benedí J
[Ad] Endereço:Departamento de Nutrición y Bromatología I. Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n., 28040-Madrid, Spain.
[Ti] Título:Fiber purified extracts of carob fruit decrease carbohydrate absorption.
[So] Source:Food Funct;8(6):2258-2265, 2017 Jun 21.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The postprandial state plays a central role in the development and setting of chronic diseases. Condensed tannins (CT) are polyphenols with a known ability to modify carbohydrate digestion and absorption. The high concentration of CT in the pulp of carob fruit suggests a potential antidiabetic effect. The aim of this work was to analyze the in vitro and in vivo effects of carob fruit extract (CFE) on the digestion and absorption of carbohydrates. α-Glucosidase activity and glucose diffusion were tested in vitro using 0.5, 1, 2 and 5 mg mL CFE concentrations. Two in vivo absorption studies, acute and subchronic, were carried out in four groups of 6 two-month-old male Wistar rats (control and CFE 25, 50 and 150 mg per kg b.w.), administering 1 mL of olive oil and 0.5 g per kg b.w. of glucose solution by oral gavage. CFE significantly inhibited α-glucosidase activity, through a competitive mechanism, from 1 mg mL , and also reduced glucose diffusion in a dose-dependent manner. In the acute study, CFE (50 and 150 mg per kg b.w.) significantly reduced the area under the curve (AUC) of blood glucose. Subchronic CFE administration induced further AUC decreases; and CFE at 150 mg per kg b.w. reduced sodium-glucose-linked transporter-1 (SGLT1) levels in the duodenum. This study demonstrates the hypoglycemic properties of CFE, highlighting its potential role as a suitable nutritional strategy in diabetic patients.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/tratamento farmacológico
Galactanos/química
Glucose/metabolismo
Inibidores de Glicosídeo Hidrolases/administração & dosagem
Hipoglicemiantes/administração & dosagem
Mananas/química
Extratos Vegetais/administração & dosagem
Gomas Vegetais/química
[Mh] Termos MeSH secundário: Animais
Diabetes Mellitus Tipo 2/enzimologia
Diabetes Mellitus Tipo 2/metabolismo
Duodeno/metabolismo
Frutas/química
Glucose/química
Seres Humanos
Cinética
Masculino
Ratos
Ratos Wistar
Transportador 1 de Glucose-Sódio/metabolismo
alfa-Glucosidases/química
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 0 (Glycoside Hydrolase Inhibitors); 0 (Hypoglycemic Agents); 0 (Mannans); 0 (Plant Extracts); 0 (Plant Gums); 0 (Slc5a1 protein, rat); 0 (Sodium-Glucose Transporter 1); EC 3.2.1.20 (alpha-Glucosidases); IY9XDZ35W2 (Glucose); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00166e


  9 / 2162 MEDLINE  
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[PMID]:28490083
[Au] Autor:Zietsman AJJ; Moore JP; Fangel JU; Willats WGT; Vivier MA
[Ad] Endereço:Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South Africa. Electronic address: jjv2@sun.ac.za.
[Ti] Título:Combining hydrothermal pretreatment with enzymes de-pectinates and exposes the innermost xyloglucan-rich hemicellulose layers of wine grape pomace.
[So] Source:Food Chem;232:340-350, 2017 Oct 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chardonnay grape pomace was treated with pressurized heat followed by enzymatic hydrolysis, with commercial or pure enzymes, in buffered conditions. The pomace was unfermented as commonly found for white winemaking wastes and treatments aimed to simulate biovalorization processing. Cell wall profiling techniques showed that the pretreatment led to depectination of the outer layers thereby exposing xylan polymers and increasing the extractability of arabinans, galactans, arabinogalactan proteins and mannans. This higher extractability is believed to be linked with partial degradation and opening-up of cell wall networks. Pectinase-rich enzyme preparations were presumably able to access the inner rhamnogalacturonan I dominant coating layers due to the hydrothermal pretreatment. Patterns of epitope abundance and the sequential release of cell wall polymers with specific combinations of enzymes led to a working model of the hitherto, poorly understood innermost xyloglucan-rich hemicellulose layers of unfermented grape pomace.
[Mh] Termos MeSH primário: Vitis
Vinho
[Mh] Termos MeSH secundário: Parede Celular
Manipulação de Alimentos
Galactanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


  10 / 2162 MEDLINE  
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[PMID]:28486974
[Au] Autor:Prakash R; Hallett IC; Wong SF; Johnston SL; O'Donoghue EM; McAtee PA; Seal AG; Atkinson RG; Schröder R
[Ad] Endereço:The New Zealand Institute for Plant & Food Research Limited (PFR), Mount Albert Research Centre, Private Bag 92169, Auckland, 1142, New Zealand.
[Ti] Título:Cell separation in kiwifruit without development of a specialised detachment zone.
[So] Source:BMC Plant Biol;17(1):86, 2017 May 10.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Unlike in abscission or dehiscence, fruit of kiwifruit Actinidia eriantha develop the ability for peel detachment when they are ripe and soft in the absence of a morphologically identifiable abscission zone. Two closely-related genotypes with contrasting detachment behaviour have been identified. The 'good-peeling' genotype has detachment with clean debonding of cells, and a peel tissue that does not tear. The 'poor-peeling' genotype has poor detachability, with cells that rupture upon debonding, and peel tissue that fragments easily. RESULTS: Structural studies indicated that peel detachability in both genotypes occurred in the outer pericarp beneath the hypodermis. Immunolabelling showed differences in methylesterification of pectin, where the interface of labelling coincided with the location of detachment in the good-peeling genotype, whereas in the poor-peeling genotype, no such interface existed. This zone of difference in methylesterification was enhanced by differential cell wall changes between the peel and outer pericarp tissue. Although both genotypes expressed two polygalacturonase genes, no enzyme activity was detected in the good-peeling genotype, suggesting limited pectin breakdown, keeping cell walls strong without tearing or fragmentation of the peel and flesh upon detachment. Differences in location and amounts of wall-stiffening galactan in the peel of the good-peeling genotype possibly contributed to this phenotype. Hemicellulose-acting transglycosylases were more active in the good-peeling genotype, suggesting an influence on peel flexibility by remodelling their substrates during development of detachability. High xyloglucanase activity in the peel of the good-peeling genotype may contribute by having a strengthening effect on the cellulose-xyloglucan network. CONCLUSIONS: In fruit of A. eriantha, peel detachability is due to the establishment of a zone of discontinuity created by differential cell wall changes in peel and outer pericarp tissues that lead to changes in mechanical properties of the peel. During ripening, the peel becomes flexible and the cells continue to adhere strongly to each other, preventing breakage, whereas the underlying outer pericarp loses cell wall strength as softening proceeds. Together these results reveal a novel and interesting mechanism for enabling cell separation.
[Mh] Termos MeSH primário: Actinidia/fisiologia
[Mh] Termos MeSH secundário: Actinidia/citologia
Actinidia/enzimologia
Actinidia/genética
Parede Celular/fisiologia
Esterificação
Frutas/fisiologia
Galactanos/metabolismo
Expressão Gênica
Genes de Plantas
Genótipo
Metilação
Monossacarídeos/metabolismo
Pectinas/metabolismo
Células Vegetais/fisiologia
Polissacarídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 0 (Monosaccharides); 0 (Pectins); 0 (Polysaccharides); 8024-50-8 (hemicellulose); 89NA02M4RX (pectin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-017-1034-2



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