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  1 / 233 MEDLINE  
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[PMID]:28738674
[Au] Autor:Mochizuki S; Morishita H; Sakurai K
[Ad] Endereço:Department of Chemistry and Biochemistry, The University of Kitakyushu , 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
[Ti] Título:Complex Consisting of ß-Glucan and Antigenic Peptides with Cleavage Site for Glutathione and Aminopeptidases Induces Potent Cytotoxic T Lymphocytes.
[So] Source:Bioconjug Chem;28(9):2246-2253, 2017 Sep 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The efficient induction of antigen-specific immune responses requires not only promotion of the uptake of antigens and adjuvant molecules into antigen-presenting cells but also control of their intracellular behavior. We previously demonstrated that the ß-glucan schizophyllan (SPG) can form complexes with CpG oligonucleotides with attached dA (CpG-dA/SPG), which can accumulate in macrophages in the draining inguinal lymph nodes and induce strong immune responses. In this study, we prepared various conjugates composed of antigenic peptide (OVA ) and dA and made complexes with SPG. The conjugates with a disulfide bond between OVA and dA were easily cleaved by glutathione. The resultant peptides with a hydrophobic amino acid at the C-terminal end was recognized by puromycin-insensitive leucine aminopeptidase (PILS-AP), which trims antigenic peptide precursors and prepares peptides of eight or nine amino acids in length, which is the optimal length for binding to major histocompatibility complex (MHC)-I. The conjugate exposed to such enzymes induced a high antigen presentation level. The antigen presentation level was almost the same before and after the complexation with SPG. Immunization with a mixture of dA-OVA /SPG and CpG-dA/SPG induced high antigen-specific cytotoxic T-lymphocyte activity at a much lower peptide dose than in previous studies. These results can be strongly ascribed to not only the cell-specific delivery by SPG but also the control of the intracellular behavior by the introduction of cleavage sites. Therefore, peptide-dA/SPG complexes could be used as potent vaccine antigens for the treatment of cancers and infectious diseases.
[Mh] Termos MeSH primário: Aminopeptidases/metabolismo
Glutationa/metabolismo
Oligodesoxirribonucleotídeos/imunologia
Ovalbumina/imunologia
Sizofirano/imunologia
Linfócitos T Citotóxicos/imunologia
[Mh] Termos MeSH secundário: Animais
Células Apresentadoras de Antígenos/imunologia
Células Apresentadoras de Antígenos/metabolismo
Controle de Doenças Transmissíveis
Doenças Transmissíveis/imunologia
Doenças Transmissíveis/metabolismo
Imunização
Macrófagos/imunologia
Camundongos
Neoplasias/imunologia
Neoplasias/metabolismo
Neoplasias/prevenção & controle
Oligodesoxirribonucleotídeos/química
Oligodesoxirribonucleotídeos/metabolismo
Ovalbumina/química
Ovalbumina/metabolismo
Sizofirano/química
Sizofirano/metabolismo
Linfócitos T Citotóxicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CPG-oligonucleotide); 0 (Oligodeoxyribonucleotides); 9006-59-1 (Ovalbumin); 9050-67-3 (Sizofiran); EC 3.4.11.- (Aminopeptidases); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00159


  2 / 233 MEDLINE  
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[PMID]:28390274
[Au] Autor:Singh MK; Kumar M; Thakur IS
[Ad] Endereço:School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
[Ti] Título:Proteomic characterization and schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena leucocephala wood under submerged fermentation.
[So] Source:Bioresour Technol;236:29-36, 2017 Jul.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study Schizophyllum commune ISTL04 was grown on Leucaena leucocephala wood (LLW) for secretome analysis and schizophyllan production. There is no report on extracellular protein profile and schizophyllan production on woody biomass by this fungus under submerged fermentation conditions. Leucaena leucocephala, a promising bioenergy crop having high holocellulose content was used as substrate without pretreatment. The maximum sugar, extracellular protein and exopolysaccharide (EPS) production during fermentation was found to be 8.53±0.07mgmL , 391±7.51mgL and 4.2±0.1gL or 0.21gg LLW on day 18 respectively. The secretome profile was dominated by glycoside hydrolases followed by carbohydrate esterase and other oxidative enzymes. EPS was further characterized by FTIR and GC-MS for functional group, monomer composition and linkage analysis and was identified as schizophyllan. The result indicated that LLW can be utilized as a low cost substrate for enzyme cocktail and schizophyllan production.
[Mh] Termos MeSH primário: Schizophyllum/enzimologia
[Mh] Termos MeSH secundário: Fermentação
Proteômica
Sizofirano
Madeira
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9050-67-3 (Sizofiran)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE


  3 / 233 MEDLINE  
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[PMID]:27807909
[Au] Autor:Ito H; Ando T; Nakamura M; Ishida H; Kanbe A; Kobiyama K; Yamamoto T; Ishii KJ; Hara A; Seishima M; Ishikawa T
[Ad] Endereço:Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
[Ti] Título:Induction of humoral and cellular immune response to hepatitis B virus (HBV) vaccine can be upregulated by CpG oligonucleotides complexed with Dectin-1 ligand.
[So] Source:J Viral Hepat;24(2):155-162, 2017 Feb.
[Is] ISSN:1365-2893
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A persistent hepatitis B virus (HBV) infection is characterized by a lack of or a weak immune response to HBV, which may be reflective of tolerance to HBV. Efficient induction of HBV-specific immune response leads to the clearance of HBV in patients with a chronic HBV infection. CpG oligodeoxynucleotides (ODN) has a powerful adjuvant effect in HBV vaccination. A recent report demonstrated that the immunization by B/K CpG ODN (K3) wrapped by the nonagonistic Dectin-1 ligand, schizophyllan (SPG), namely K3-SPG, was more effective in the induction of antigen-specific immune response than that by K3. In this study, we examined the efficacy of K3-SPG as a HBV vaccine adjuvant. Wild-type (WT) mice and HBV transgenic (HBV-Tg) mice were subcutaneously immunized with hepatitis B surface antigen (HBsAg) alone, HBsAg and K3, or HBsAg and K3-SPG. The vaccination with HBsAg and K3-SPG significantly enhanced humoral and cellular immune response to HBV antigen compared to the other vaccinations in WT and HBV-Tg mice. K3-SPG induced the accumulation of dendritic cells (DCs) into draining lymph node and the activation of DCs. The expression of cytokines and chemokines related to Th1 and Th2 responses was upregulated after the vaccination including with K3-SPG. In conclusion, these results indicated that the vaccination using K3-SPG may overcome tolerance even in patients with chronic HBV infection.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Vacinas contra Hepatite B/administração & dosagem
Vacinas contra Hepatite B/imunologia
Imunidade Celular
Imunidade Humoral
Oligodesoxirribonucleotídeos/administração & dosagem
Sizofirano/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Antígenos de Superfície da Hepatite B/administração & dosagem
Antígenos de Superfície da Hepatite B/imunologia
Injeções Subcutâneas
Masculino
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (CPG-oligonucleotide); 0 (Hepatitis B Surface Antigens); 0 (Hepatitis B Vaccines); 0 (Oligodeoxyribonucleotides); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.1111/jvh.12629


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[PMID]:27443590
[Au] Autor:Jamshidian H; Shojaosadati SA; Vilaplana F; Mousavi SM; Soudi MR
[Ad] Endereço:Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran.
[Ti] Título:Characterization and optimization of schizophyllan production from date syrup.
[So] Source:Int J Biol Macromol;92:484-493, 2016 Nov.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture. Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively. Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues. Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
[Mh] Termos MeSH primário: Phoeniceae/química
Sizofirano/biossíntese
[Mh] Termos MeSH secundário: Agricultura
Biomassa
Carbono/farmacologia
Meios de Cultura/química
Glicosídeos/química
Espectroscopia de Ressonância Magnética
Modelos Teóricos
Peso Molecular
Monossacarídeos/análise
Nitrogênio/farmacologia
Phoeniceae/efeitos dos fármacos
Polissacarídeos/análise
Espectroscopia de Infravermelho com Transformada de Fourier
Fatores de Tempo
Zea mays/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Glycosides); 0 (Monosaccharides); 0 (Polysaccharides); 7440-44-0 (Carbon); 9050-67-3 (Sizofiran); N762921K75 (Nitrogen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


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[PMID]:27035516
[Au] Autor:Izumi H; Nagao S; Mochizuki S; Fujiwara N; Sakurai K; Morimoto Y
[Ad] Endereço:University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan.
[Ti] Título:Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system.
[So] Source:Int J Oncol;48(6):2472-8, 2016 Jun.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Silencing Y-box binding protein 1 (YB-1) can be an excellent target for cancer therapy and many lung cancer cells express the polysaccharide-recognition receptor Dectin-1. We designed a Dectin-1 targeting vehicle delivering YB-1-antisense DNA. First, we selected five optimal antisense DNA sequences to silence YB-1 from among 153 candidates. We chose the sequence closest to the start codon (AS014), and attached dA40 to the 3' end; dA40 promotes complex formation with a ß-(1➝3)-d-glucan called schizophyllan (SPG). The resultant complexes were applied to 12 human-oriented lung cancer cell lines, and cell viability was examined. The cell lines exhibited decreased viability and showed strong affinity to bind SPG, suggesting the AS014/SPG complex entered the cells via the Dectin-1 mediated pathway.
[Mh] Termos MeSH primário: DNA Antissenso/farmacologia
Lectinas Tipo C/química
Neoplasias Pulmonares/genética
Sizofirano/química
Proteína 1 de Ligação a Y-Box/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
DNA Antissenso/química
DNA Antissenso/genética
Sistemas de Liberação de Medicamentos
Inativação Gênica
Seres Humanos
Proteína 1 de Ligação a Y-Box/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Antisense); 0 (Lectins, C-Type); 0 (Y-Box-Binding Protein 1); 0 (YBX1 protein, human); 0 (dectin 1); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2016.3451


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[PMID]:26946401
[Au] Autor:Kim YR; Hwang J; Koh HJ; Jang K; Lee JD; Choi J; Yang CS
[Ad] Endereço:Department of Molecular and Life Science, Hanyang University, Ansan 15588, Republic of Korea.
[Ti] Título:The targeted delivery of the c-Src peptide complexed with schizophyllan to macrophages inhibits polymicrobial sepsis and ulcerative colitis in mice.
[So] Source:Biomaterials;89:1-13, 2016 May.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hyper-inflammatory responses triggered by intracellular reactive oxygen species (ROS) can lead to a variety of diseases, including sepsis and colitis. However, the regulators of this process remain poorly defined. In this study, we demonstrate that c-Src is a negative regulator of cellular ROS generation through its binding to p47phox. This molecule also competitively inhibits the NADPH oxidase complex (NOX) assembly. Furthermore, we developed the schizophyllan (SPG)-c-Src SH3 peptide, which is a ß-1,3-glucan conjugated c-Src SH3-derived peptide composed of amino acids 91-108 and 121-140 of c-Src. The SPG-SH3 peptide has a significant therapeutic effect on mouse ROS-mediated inflammatory disease models, cecal-ligation-puncture-induced sepsis, and dextran sodium sulfate-induced colitis. It does so by inhibiting the NOX subunit assembly and proinflammatory mediator production. Therefore, the SPG-SH3 peptide is a potential therapeutic agent for ROS-associated lethal inflammatory diseases. Our findings provide clues for the development of new peptide-base drugs that will target p47phox.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/uso terapêutico
Colite Ulcerativa/tratamento farmacológico
Macrófagos/efeitos dos fármacos
Oligopeptídeos/uso terapêutico
Sepse/tratamento farmacológico
Sizofirano/uso terapêutico
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Adjuvantes Imunológicos/química
Sequência de Aminoácidos
Animais
Linhagem Celular
Colite Ulcerativa/imunologia
Colite Ulcerativa/microbiologia
Sistemas de Liberação de Medicamentos
Feminino
Células HEK293
Seres Humanos
Macrófagos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
NADPH Oxidases/imunologia
Oligopeptídeos/administração & dosagem
Oligopeptídeos/química
Espécies Reativas de Oxigênio/imunologia
Sepse/imunologia
Sepse/microbiologia
Sizofirano/administração & dosagem
Sizofirano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Oligopeptides); 0 (Reactive Oxygen Species); 0 (Src peptide); 9050-67-3 (Sizofiran); EC 1.6.3.- (NADPH Oxidases); EC 1.6.3.1 (neutrophil cytosolic factor 1)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160307
[St] Status:MEDLINE


  7 / 233 MEDLINE  
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[PMID]:25830418
[Au] Autor:Sutivisedsak N; Leathers TD; Biresaw G; Nunnally MS; Bischoff KM
[Ad] Endereço:a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
[Ti] Título:Simplified process for preparation of schizophyllan solutions for biomaterial applications.
[So] Source:Prep Biochem Biotechnol;46(3):313-9, 2016.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Schizophyllan is a biopolymer commercially produced for pharmaceutical and cosmetics uses. However, schizophyllan also has potential biomaterial applications. Schizophyllan is conventionally produced from glucose and recovered by diafiltration and ultrafiltration to produce a highly purified product. Here we demonstrate a simplified process for preparation of schizophyllan solutions for biomaterial applications. Schizophyllan was produced in 1.5-L bioreactors from distiller's dried grains with solubles (DDGS), an abundant coproduct of dry grind fuel ethanol production. Downstream processing eliminated filtration and concentration steps, providing solutions containing 4.2 ± 0.3 g schizophyllan/L. Solutions contained high-molecular-weight schizophyllan and exhibited viscosity properties similar to those of commercial schizophyllan. Schizophyllan solutions showed promise as a component of biolubricants in friction and wear tests and by dynamic surface and interfacial tension measurements.
[Mh] Termos MeSH primário: Materiais Biocompatíveis
Sizofirano/química
[Mh] Termos MeSH secundário: Reatores Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150402
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2015.1031392


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[PMID]:26562685
[Au] Autor:Mochizuki S; Morishita H; Kobiyama K; Aoshi T; Ishii KJ; Sakurai K
[Ad] Endereço:Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan. Electronic address: mochizuki@kitakyu-u.ac.jp.
[Ti] Título:Immunization with antigenic peptides complexed with ß-glucan induces potent cytotoxic T-lymphocyte activity in combination with CpG-ODNs.
[So] Source:J Control Release;220(Pt A):495-502, 2015 Dec 28.
[Is] ISSN:1873-4995
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The induction of antigen-specific immune responses requires immunization with not only antigens, but also adjuvants. CpG oligonucleotides (CpG-ODNs) are well-known ligands for Toll-like receptor 9 and a potent adjuvant that induces both Th1-type humoral and cellular immune responses including cytotoxic T-lymphocyte responses. We previously demonstrated that ß-glucan schizophyllan (SPG) can form complexes with CpG-ODNs with attached dA40 (CpG-dA/SPG), which can accumulate in macrophages in the draining inguinal lymph nodes and induce strong immune responses by co-administration of antigenic proteins, namely ovalbumin (OVA). Immunization with antigenic peptides, OVA257-264, did not induce these antigen-specific immune responses even in combination with CpG-dA/SPG, indicating that peptides require a carrier to antigen presenting cells. In this study, we prepared conjugates comprising OVA257-264 and dA40, and made complexes with SPG. Immunization with OVA257-264-dA/SPG induced peptide-specific immune responses in combination with CpG-dA regardless of complexation with SPG both in vitro and in vivo. When splenocytes from immunized mice were incubated with E.G7-OVA tumor model cells presenting OVA peptides, the number of cells drastically decreased after 24h. Furthermore, mice pre-immunized with OVA257-264-dA/SPG and CpG-ODNs exhibited a long delay in tumor growth after tumor inoculation. Therefore, these peptide-dA/SPG and CpG-dA/SPG complexes could be used as a potent vaccine for the treatment of cancers and infectious diseases.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Vacinas Anticâncer/administração & dosagem
Imunização
Ativação Linfocitária/efeitos dos fármacos
Linfócitos do Interstício Tumoral/efeitos dos fármacos
Neoplasias/tratamento farmacológico
Oligodesoxirribonucleotídeos/administração & dosagem
Ovalbumina/administração & dosagem
Sizofirano/administração & dosagem
Linfócitos T Citotóxicos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/farmacocinética
Animais
Células Apresentadoras de Antígenos/efeitos dos fármacos
Células Apresentadoras de Antígenos/imunologia
Células Apresentadoras de Antígenos/metabolismo
Vacinas Anticâncer/imunologia
Vacinas Anticâncer/farmacocinética
Células Cultivadas
Linfócitos do Interstício Tumoral/imunologia
Linfócitos do Interstício Tumoral/metabolismo
Linfócitos do Interstício Tumoral/patologia
Camundongos Endogâmicos C57BL
Neoplasias/imunologia
Neoplasias/metabolismo
Neoplasias/patologia
Oligodesoxirribonucleotídeos/imunologia
Oligodesoxirribonucleotídeos/farmacocinética
Ovalbumina/imunologia
Ovalbumina/farmacocinética
Fragmentos de Peptídeos/administração & dosagem
Fragmentos de Peptídeos/imunologia
Fragmentos de Peptídeos/farmacocinética
Sizofirano/imunologia
Sizofirano/farmacocinética
Baço/efeitos dos fármacos
Baço/imunologia
Baço/metabolismo
Linfócitos T Citotóxicos/imunologia
Linfócitos T Citotóxicos/metabolismo
Distribuição Tecidual
Carga Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (CPG-oligonucleotide); 0 (Cancer Vaccines); 0 (OVA-8); 0 (Oligodeoxyribonucleotides); 0 (Peptide Fragments); 9006-59-1 (Ovalbumin); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151113
[St] Status:MEDLINE


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[PMID]:26126943
[Au] Autor:Zhong K; Tong L; Liu L; Zhou X; Liu X; Zhang Q; Zhou S
[Ad] Endereço:Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, PR China; Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, PR China.
[Ti] Título:Immunoregulatory and antitumor activity of schizophyllan under ultrasonic treatment.
[So] Source:Int J Biol Macromol;80:302-8, 2015 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aim of this study was to investigate the effect of ultrasonic treatment on the biological activities of schizophyllan (SPG) from Schizophyllum commune. The immunoregulatory and antitumor activity in vitro and in vivo of SPG and ultrasonic-treated SPG (USPG) were evaluated by splenic lymphocytes, macrophages RAW264.7 and human breast carcinoma T-47D cells. Compared with SPG, USPG fractions had small molecular weight and narrow distribution. Meantime, more enhancement of NO production in macrophages RAW264.7, lymphocytes proliferation rates, IL-2 and TNF-α level from spleen lymphocytes and T-47D cells inhibition rates were observed in USPG fractions groups. This result indicated that the immune-enhancing and antitumor activity of SPG was significantly improved after ultrasonic treatment. USPG60 exhibited the highest biological activity in this study. In conclusion, application of ultrasonic technology on SPG preparation is an efficient approach to get high biological polysaccharide, and USPG60 might be a potential functional component for immunoregulatory and cancer treatment.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Fatores Imunológicos/farmacologia
Sizofirano/farmacologia
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Fatores Imunológicos/química
Interleucina-2/secreção
Ativação Linfocitária/efeitos dos fármacos
Linfócitos/citologia
Linfócitos/efeitos dos fármacos
Linfócitos/imunologia
Linfócitos/metabolismo
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Macrófagos/metabolismo
Masculino
Camundongos
Peso Molecular
Óxido Nítrico/biossíntese
Células RAW 264.7
Ratos
Schizophyllum/química
Sizofirano/química
Baço/efeitos dos fármacos
Baço/imunologia
Fator de Necrose Tumoral alfa/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Immunologic Factors); 0 (Interleukin-2); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150831
[Lr] Data última revisão:
150831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150702
[St] Status:MEDLINE


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[PMID]:25567536
[Au] Autor:Zhang Q; Ichimaru N; Higuchi S; Cai S; Hou J; Fujino M; Nonomura N; Kobayashi M; Ando H; Uno A; Sakurai K; Mochizuki S; Adachi Y; Ohno N; Zou H; Xu J; Li XK; Takahara S
[Ad] Endereço:1] Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan [2] Huashan Hospital, Fudan University, Shanghai, China.
[Ti] Título:Permanent acceptance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system.
[So] Source:Gene Ther;22(3):217-26, 2015 Mar.
[Is] ISSN:1476-5462
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The CD40/CD154 co-stimulatory pathway is crucial in alloimmune response. We developed a novel small interfering RNA (siRNA) delivery system with a poly-dA extension at the 5'-end of the siRNA sense strand that was stably incorporated into 1,3-ß-glucan (schizophyllan, SPG). This was captured and incorporated into dendritic cells (DCs) through its receptor, Dectin-1, specifically silencing CD40 genes (siCD40) to exert immunoregulatory activity. siCD40/SPG-treated CBA mice permanently accepted B10 fully mismatched cardiac allografts. Consistent with graft survival, the infiltration of CD4(+), CD8(+) T cells into the graft was lower, and that the numbers of CD40(low)CD11c(+) DCs cells and CD4(+)Foxp3(+)cells were increased in both the graft and in the recipient spleen. In addition, naive CBA recipients given an adoptive transfer of splenocytes from the primary recipients with siCD40/SPG accepted a heart graft from donor-type B10, but not third-party Balb/c mice. In conclusion, the treatment with siCD40/SPG targeting DCs could generate antigen-specific Tregs, resulting in the permanent acceptance of mouse cardiac allografts. These findings have important implications for clarifying the mechanism underlying the induction of tolerance in DCs, and also highlight the potential of immunomodulation and the feasibility of siRNA-based clinical therapy in the transplantation field.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/metabolismo
Aloenxertos/fisiologia
Antígenos CD40/metabolismo
Transplante de Coração
Células Mieloides/metabolismo
RNA Interferente Pequeno/metabolismo
Sizofirano/metabolismo
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/química
Aloenxertos/citologia
Animais
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Células Cultivadas
Células Dendríticas/imunologia
Modelos Animais de Doenças
Masculino
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos CBA
RNA Interferente Pequeno/química
RNA Interferente Pequeno/genética
Sizofirano/química
Subpopulações de Linfócitos T/imunologia
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (CD40 Antigens); 0 (RNA, Small Interfering); 9050-67-3 (Sizofiran)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150109
[St] Status:MEDLINE
[do] DOI:10.1038/gt.2014.119



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