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[PMID]:28682358
[Ti] Título:Pulsars still dazzle after 50 years.
[So] Source:Nature;547(7661):5-6, 2017 07 04.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: DEAE-Dextrano
Estimulação Luminosa
[Mh] Termos MeSH secundário: Seres Humanos
Luz
Percepção Visual
[Pt] Tipo de publicação:EDITORIAL; COMMENT
[Nm] Nome de substância:
9015-73-0 (DEAE-Dextran)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1038/547005b


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[PMID]:28328957
[Au] Autor:Hong ZW; Yang YC; Pan T; Tzeng HF; Fu HW
[Ad] Endereço:Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
[Ti] Título:Differential effects of DEAE negative mode chromatography and gel-filtration chromatography on the charge status of Helicobacter pylori neutrophil-activating protein.
[So] Source:PLoS One;12(3):e0173632, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Helicobacter pylori neutrophil-activating protein (HP-NAP) is involved in H. pylori-associated gastric inflammation. HP-NAP is also a vaccine candidate, a possible drug target, and a potential diagnostic marker for H. pylori-associated diseases. Previously, we purified recombinant HP-NAP by one-step diethylaminoethyl (DEAE) negative mode chromatography by collecting the unbound fraction at pH 8.0 at 4°C. It remains unclear why HP-NAP does not bind to DEAE resins at the pH above its isoelectric point during the purification. To investigate how pH affects the surface net charge of HP-NAP and its binding to DEAE resins during the purification, recombinant HP-NAP expressed in Escherichia coli was subjected to DEAE negative mode chromatography at pH ranging from 7.0 to 9.0 at 25°C and the surface charge of purified HP-NAP was determined by capillary electrophoresis. A minimal amount of HP-NAP was detected in the elution fraction of DEAE Sepharose resin at pH 8.5, whereas recombinant HP-NAP was detected in the elution fraction of DEAE Sephadex resin only at pH 7.0 and 8.0. The purified recombinant HP-NAP obtained from the unbound fractions was not able to bind to DEAE resins at pH 7.0 to 9.0. In addition, the surface charge of the purified HP-NAP was neutral at pH 7.0 to 8.0 and was either neutral or slightly negative at pH 8.5 and 9.0. However, recombinant HP-NAP purified from gel-filtration chromatography was able to bind to DEAE Sepharose resin at pH 7.0 to 9.0 and DEAE Sephadex resin at pH 7.0. At pH 8.5 and 9.0, only the negatively charged species of HP-NAP were found. Thus, recombinant HP-NAP with different charge status can be differentially purified by DEAE negative mode chromatography and gel-filtration chromatography. Furthermore, the charge distribution on the surface of HP-NAP, the presence of impure proteins, and the overall net charge of the resins all affect the binding of HP-NAP to DEAE resins during the negative purification.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/isolamento & purificação
Helicobacter pylori/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/imunologia
Cromatografia em Gel
Cromatografia por Troca Iônica
DEAE-Dextrano
Eletroquímica
Eletroforese Capilar
Etanolaminas
Infecções por Helicobacter/diagnóstico
Infecções por Helicobacter/imunologia
Helicobacter pylori/imunologia
Helicobacter pylori/patogenicidade
Seres Humanos
Concentração de Íons de Hidrogênio
Resinas de Troca Iônica
Neutrófilos/imunologia
Proteínas Recombinantes/química
Proteínas Recombinantes/imunologia
Proteínas Recombinantes/isolamento & purificação
Sefarose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Ethanolamines); 0 (Ion Exchange Resins); 0 (Recombinant Proteins); 0 (neutrophil-activating protein A, Helicobacter pylori); 9012-36-6 (Sepharose); 9015-73-0 (DEAE-Dextran); S6DL4M053U (2-diethylaminoethanol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173632


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[PMID]:27779713
[Au] Autor:Sonoda A; Nitta N; Tsuchiya K; Nitta-Seko A; Ohta S; Otani H; Murata K
[Ad] Endereço:Department of Radiology, Shiga University of Medical Science, Otsu, Shiga 520­2192, Japan.
[Ti] Título:A novel blood-pooling MR contrast agent: Carboxymethyl-diethylaminoethyl dextran magnetite.
[So] Source:Mol Med Rep;14(6):5195-5198, 2016 Dec.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Gadofosveset trisodium is available as a prolonged pooling vascular contrast agent for magnetic resonance imaging. As gadolinium (Gd)-based agents may increase the risk for nephrogenic systemic fibrosis in patients with severe renal insufficiency, the present study synthesized carboxymethyl-diethylaminoethyl dextran magnetite (CMEADM) particles as a blood-pooling, non-Gd­based contrast agent. CMEADM particles carry a negative or positive charge due to the binding of amino and carboxyl groups to the hydroxyl group of dextran. The present study evaluated whether the degree of charge alters the blood­pooling time. The evaluation was performed by injecting four groups of three Japanese white rabbits each with CMEADM­, CMEADM2­, CMEADM+ (surface charges: ­10.4, ­41.0 and +9.6 mV, respectively) or with ultrasmall superparamagnetic iron oxide (USPIO; ­11.5 mV). The relative signal intensity (SIrel) of each was calculated using the following formula: SIrel = (SI post­contrast ­ SI pre­contrast / SI pre­contrast) x 100. Following injection with the CMEADMs, but not with USPIO, the in vivo pooling time was prolonged to >300 min. No significant differences were attributable to the electric charge among the CMEADM­, CMEADM2­ or and CMEADM+ particles when analyzed with analysis of variance and Tukey's HSD test. Taken together, all three differently­charged CMEADM2 particles exhibited prolonged vascular enhancing effects, compared with the USPIO. The degree of charge of the contrast agents used in the present study did not result in alteration of the prolonged blood pooling time.
[Mh] Termos MeSH primário: Meios de Contraste
Imagem por Ressonância Magnética
[Mh] Termos MeSH secundário: Animais
Meios de Contraste/administração & dosagem
Meios de Contraste/química
DEAE-Dextrano/química
DEAE-Dextrano/metabolismo
Feminino
Óxido Ferroso-Férrico/química
Óxido Ferroso-Férrico/metabolismo
Angiografia por Ressonância Magnética
Imagem por Ressonância Magnética/métodos
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 9015-73-0 (DEAE-Dextran); XM0M87F357 (Ferrosoferric Oxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5874


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[PMID]:27260131
[Au] Autor:Abioye AO; Chi GT; Simone E; Nagy Z
[Ad] Endereço:Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, UK. Electronic address: aabioye@dmu.ac.uk.
[Ti] Título:Real-time monitoring of the mechanism of ibuprofen-cationic dextran crystanule formation using crystallization process informatics system (CryPRINS).
[So] Source:Int J Pharm;509(1-2):264-278, 2016 Jul 25.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:One step aqueous melt-crystallization and in situ granulation was utilized to produce ibuprofen-cationic dextran [diethylaminoethyl dextran (Ddex)] conjugate crystanules without the use of surfactants or organic solvents. This study investigates the mechanism of in situ granulation-induced crystanule formation using ibuprofen (Ibu) and Ddex. Laboratory scale batch aqueous crystallization system containing in situ monitoring probes for particle vision measurement (PVM), UV-vis measurement and focused beam reflectance measurements (FBRM) was adapted using pre-defined formulation and process parameters. Pure ibuprofen showed nucleation domain between 25 and 64°C, producing minicrystals with onset of melting at 76°C and enthalpy of fusion (ΔH) of 26.22kJ/mol. On the other hand Ibu-Ddex crystanules showed heterogeneous nucleation which produced spherical core-shell structure. PVM images suggest that internalization of ibuprofen in Ddex corona occurred during the melting phase (before nucleation) which inhibited crystal growth inside the Ddex corona. The remarkable decrease in ΔH of the crystanules from 26.22 to 11.96kJ/mol and the presence of broad overlapping DSC thermogram suggests formation of ibuprofen-Ddex complex and crystalline-amorphous transformation. However Raman and FTIR spectra did not show any significant chemical interaction between ibuprofen and Ddex. A significant increase in dissolution efficiency from 45 to 81% within 24h and reduced burst release provide evidence for potential application of crystanules in controlled drug delivery systems. It was evident that in situ granulation of ibuprofen inhibited the aqueous crystallization process. It was concluded that in situ granulation-aqueous crystallization technique is a novel unit operation with potential application in continuous pharmaceutical processing.
[Mh] Termos MeSH primário: Cátions/química
DEAE-Dextrano/química
Dextranos/química
Ibuprofeno/química
[Mh] Termos MeSH secundário: Química Farmacêutica/métodos
Cristalização/métodos
Preparações de Ação Retardada/química
Portadores de Fármacos/química
Composição de Medicamentos/métodos
Sistemas de Liberação de Medicamentos/métodos
Estabilidade de Medicamentos
Tamanho da Partícula
Solubilidade
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Delayed-Action Preparations); 0 (Dextrans); 0 (Drug Carriers); 9015-73-0 (DEAE-Dextran); WK2XYI10QM (Ibuprofen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160605
[St] Status:MEDLINE


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[PMID]:26915059
[Au] Autor:Türkes C; Söyüt H; Beydemir S
[Ad] Endereço:Department of Chemistry, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.
[Ti] Título:In vitro inhibitory effects of palonosetron hydrochloride, bevacizumab and cyclophosphamide on purified paraoxonase-I (hPON1) from human serum.
[So] Source:Environ Toxicol Pharmacol;42:252-7, 2016 Mar.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we investigated the effects of the drugs, palonosetron hydrochloride, bevacizumab and cyclophosphamide, on human serum paraoxonase-I (hPON1) enzyme activity in in vitro conditions. The enzyme was purified ∼231-fold with 34.2% yield by using ammonium sulphate precipitation, DEAE-Sephadex A-50 ion-exchange chromatography and Sephadex G-200 gel-filtration chromatography from human serum. hPON1 exhibited a single protein band on the SDS polyacrylamide gel electrophoresis. The inhibition studies were performed on paraoxonase activity of palonosetron hydrochloride, bevacizumab and cyclophosphamide. Ki constants were found as 0.033±0.001, 0.054±0.003 mM and 3.419±0.518 mM, respectively. Compared to the inhibition rates of the drugs, palonosetron hydrochloride has the maximum inhibition rate. However, inhibition mechanisms of the drugs were determined as noncompetitive by Lineweaver-Burk curves.
[Mh] Termos MeSH primário: Arildialquilfosfatase/metabolismo
Bevacizumab/farmacologia
Ciclofosfamida/farmacologia
Inibidores Enzimáticos/farmacologia
Isoquinolinas/farmacologia
Quinuclidinas/farmacologia
[Mh] Termos MeSH secundário: Cromatografia em Gel
DEAE-Dextrano/análogos & derivados
Dextranos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dextrans); 0 (Enzyme Inhibitors); 0 (Isoquinolines); 0 (Quinuclidines); 2S9ZZM9Q9V (Bevacizumab); 39455-31-7 (DEAE-Sephadex A-50); 5D06587D6R (palonosetron); 8N3DW7272P (Cyclophosphamide); 9014-76-0 (sephadex); 9015-73-0 (DEAE-Dextran); EC 3.1.8.1 (Aryldialkylphosphatase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE


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[PMID]:25826253
[Au] Autor:Abioye AO; Kola-Mustapha A
[Ad] Endereço:a Leicester School of Pharmacy, De Montfort University , The Gateway , Leicester , United Kingdom.
[Ti] Título:Formulation studies on ibuprofen sodium-cationic dextran conjugate: effect on tableting and dissolution characteristics of ibuprofen.
[So] Source:Drug Dev Ind Pharm;42(1):39-59, 2016 Jan.
[Is] ISSN:1520-5762
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of electrostatic interaction between ibuprofen sodium (IbS) and cationic diethylaminoethyl dextran (Ddex), on the tableting properties and ibuprofen release from the conjugate tablet was investigated. Ibuprofen exhibits poor flow, compaction (tableting) and dissolution behavior due to its hydrophobic structure, high cohesive, adhesive and viscoelastic properties therefore it was granulated with cationic Ddex to improve its compression and dissolution characteristics. Electrostatic interaction and hydrogen bonding between IbS and Ddex was confirmed with FT-IR and DSC results showed a stepwise endothermic solid-solid structural transformation from racemic to anhydrous forms between 120 and 175 °C which melted into liquid form at 208.15 °C. The broad and diffused DSC peaks of the conjugate granules as well as the disappearance of ibuprofen melting peak provided evidence for their highly amorphous state. It was evident that Ddex improved the flowability and densification of the granules and increased the mechanical and tensile strengths of the resulting tablets as the tensile strength increased from 0.67 ± 0.0172 to 1.90 ± 0.0038 MPa with increasing Ddex concentration. Both tapping and compression processes showed that the most prominent mechanism of densification were particle slippage, rearrangement and plastic deformation while fragmentation was minimized. Ddex retarded the extent of dissolution in general, indicating potentials for controlled release formulations. Multiple release mechanisms including diffusion; anomalous transport and super case II transport were noted. It was concluded that interaction between ibuprofen sodium and Ddex produced a novel formulation with improved flowability, tableting and dissolution characteristics with potential controlled drug release characteristics dictated by Ddex concentration.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/química
DEAE-Dextrano/química
Composição de Medicamentos/métodos
Liberação Controlada de Fármacos
Ibuprofeno/química
[Mh] Termos MeSH secundário: Anti-Inflamatórios não Esteroides/administração & dosagem
Anti-Inflamatórios não Esteroides/farmacocinética
Varredura Diferencial de Calorimetria
Cátions
Preparações de Ação Retardada/química
Portadores de Fármacos/química
Ligações de Hidrogênio
Ibuprofeno/administração & dosagem
Ibuprofeno/farmacocinética
Tamanho da Partícula
Solubilidade
Espectroscopia de Infravermelho com Transformada de Fourier
Eletricidade Estática
Comprimidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cations); 0 (Delayed-Action Preparations); 0 (Drug Carriers); 0 (Tablets); 9015-73-0 (DEAE-Dextran); WK2XYI10QM (Ibuprofen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150401
[St] Status:MEDLINE


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[PMID]:25662061
[Au] Autor:Li J; Song CX; Li YP; Li L; Wei XH; Wang JL; Liu XS
[Ad] Endereço:Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
[Ti] Título:Rab3 is involved in cellular immune responses of the cotton bollworm, Helicoverpa armigera.
[So] Source:Dev Comp Immunol;50(2):78-86, 2015 Jun.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rab3, a member of the Rab GTPase family, has been found to be involved in innate immunity. However, the precise function of this GTPase in innate immunity remains unknown. In this study, we identified a Rab3 gene (Ha-Rab3) from the cotton bollworm, Helicoverpa armigera and studied its roles in innate immune responses. Expression of Ha-Rab3 was upregulated in the hemocytes of H. armigera larvae after the injection of Escherichia coli or chromatography beads. The dsRNA-mediated knockdown of Ha-Rab3 gene in H. armigera larval hemocytes led to significant reduction in the phagocytosis and nodulation activities of hemocytes against E. coli, significant increase in the bacterial load in larval hemolymph, and significant reduction in the encapsulation activities of hemocytes toward invading chromatography beads. Furthermore, Ha-Rab3 knockdown significantly suppressed spreading of plasmatocytes. These results suggest that Ha-Rab3 plays important roles in H. armigera cellular immune responses, possibly by mediating spreading of hemocytes.
[Mh] Termos MeSH primário: Hemócitos/imunologia
Larva/imunologia
Mariposas/imunologia
Proteínas rab3 de Ligação ao GTP/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Carga Bacteriana/genética
Carga Bacteriana/imunologia
Sequência de Bases
DEAE-Dextrano/imunologia
Escherichia coli/imunologia
Hemolinfa/imunologia
Imunidade Celular/imunologia
Imunidade Inata
Microesferas
Dados de Sequência Molecular
Fagocitose/genética
Fagocitose/imunologia
Interferência de RNA
RNA Interferente Pequeno
Proteínas Recombinantes/genética
Análise de Sequência de DNA
Proteínas rab3 de Ligação ao GTP/biossíntese
Proteínas rab3 de Ligação ao GTP/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Small Interfering); 0 (Recombinant Proteins); 9015-73-0 (DEAE-Dextran); EC 3.6.5.2 (rab3 GTP-Binding Proteins)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150324
[Lr] Data última revisão:
150324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE


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[PMID]:25527142
[Au] Autor:Abioye AO; Kola-Mustapha A
[Ad] Endereço:Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, UK, aabioye@dmu.ac.uk.
[Ti] Título:Controlled Electrostatic Self-Assembly of Ibuprofen-Cationic Dextran Nanoconjugates Prepared by low Energy Green Process - a Novel Delivery Tool for Poorly Soluble Drugs.
[So] Source:Pharm Res;32(6):2110-31, 2015 Jun.
[Is] ISSN:1573-904X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The direct effect of electrostatic interaction between ibuprofen and cationic dextran on the system-specific physicochemical parameters and intrinsic dissolution characteristics of ibuprofen was evaluated in order to develop drug-polymer nanoconjugate as a delivery strategy for poorly soluble drugs. METHODS: Amorphous ibuprofen-DEAE dextran (Ddex) nanoconjugate was prepared using a low energy, controlled amphiphile-polyelectrolyte electrostatic self-assembly technique optimized by ibuprofen critical solubility and Ddex charge screening. Physicochemical characteristics of the nanoconjugates were evaluated using FTIR, DSC, TGA, NMR and SEM relative to pure ibuprofen. The in vitro release profiles and mechanism of ibuprofen release were determined using mathematical models including zero and first order kinetics; Higuchi; Hixson-Crowell and Korsmeyer-Peppas. RESULTS: Electrostatic interaction between ibuprofen and Ddex was confirmed with FT-IR, (1)H NMR and (13)C NMR spectroscopy. The broad and diffused DSC peaks of the nanoconjugate as well as the disappearance of ibuprofen melting peak provided evidence for their highly amorphous state. Low concentrations of Ddex up to 1.0 × 10(-6) g/dm(3) enhanced dissolution of ibuprofen to a maximum of 81.32% beyond which retardation occurred steadily. Multiple release mechanisms including diffusion; discrete drug dissolution; anomalous transport and super case II transport were noted. CONCLUSIONS: Controlled assembly of ibuprofen and Ddex produced a novel formulation with potential extended drug release dictated by Ddex concentration.
[Mh] Termos MeSH primário: DEAE-Dextrano/química
Portadores de Fármacos
Química Verde
Ibuprofeno/química
Nanopartículas
Tecnologia Farmacêutica/métodos
[Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria
Cátions
Química Farmacêutica
Preparações de Ação Retardada
Cinética
Espectroscopia de Ressonância Magnética
Microscopia Eletrônica de Varredura
Nanotecnologia
Solubilidade
Espectroscopia de Infravermelho com Transformada de Fourier
Eletricidade Estática
Termogravimetria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Delayed-Action Preparations); 0 (Drug Carriers); 9015-73-0 (DEAE-Dextran); WK2XYI10QM (Ibuprofen)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141221
[St] Status:MEDLINE
[do] DOI:10.1007/s11095-014-1603-0


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[PMID]:25394479
[Au] Autor:Zarogoulidis P; Hohenforst-Schmidt W; Huang H; Sahpatzidou D; Freitag L; Sakkas L; Rapti A; Kioumis I; Pitsiou G; Kouzi-Koliakos K; Papamichail A; Papaiwannou A; Tsiouda T; Tsakiridis K; Porpodis K; Lampaki S; Organtzis J; Gschwendtner A; Zarogoulidis K
[Ad] Endereço:Pulmonary Department, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece. pzarog@hotmail.com.
[Ti] Título:A gene therapy induced emphysema model and the protective role of stem cells.
[So] Source:Diagn Pathol;9:195, 2014 Nov 14.
[Is] ISSN:1746-1596
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chronic obstructive pulmonary disease presents with two different phenotypes: chronic bronchitis and emphysema with parenchymal destruction. Decreased expression of vascular endothelial growth factor and increased endothelial cell apoptosis are considered major factors for emphysema. Stem cells have the ability of vascular regeneration and function as a repair mechanism for the damaged endothelial cells. Currently, minimally invasive interventional procedures such as placement of valves, bio-foam or coils are performed in order to improve the disturbed mechanical function in emphysema patients. However, these procedures cannot restore functional lung tissue. Additionally stem cell instillation into the parenchyma has been used in clinical studies aiming to improve overall respiratory function and quality of life. METHODS: In our current experiment we induced emphysema with a DDMC non-viral vector in BALBC mice and simultaneously instilled stem cells testing the hyposthesis that they might have a protective role against the development of emphysema. The mice were divided into four groups: a) control, b) 50.000 cells, c) 75.000 and d) 100.000 cells. RESULTS: Lung pathological findings revealed that all treatment groups had less damage compared to the control group. Additionally, we observed that emphysema lesions were less around vessels in an area of 10 µm. CONCLUSIONS: Our findings indicate that stem cell instillation can have a regenerative role if applied upon a tissue scaffold with vessel around. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_195.
[Mh] Termos MeSH primário: DEAE-Dextrano
Pulmão/irrigação sanguínea
Pulmão/patologia
Transplante de Células-Tronco Mesenquimais
Células Mesenquimais Estromais
Enfisema Pulmonar/prevenção & controle
Regeneração
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Modelos Animais de Doenças
Genes Reporter
Seres Humanos
Pulmão/metabolismo
Células Mesenquimais Estromais/metabolismo
Camundongos Endogâmicos BALB C
Enfisema Pulmonar/induzido quimicamente
Enfisema Pulmonar/metabolismo
Enfisema Pulmonar/patologia
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9015-73-0 (DEAE-Dextran)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141115
[St] Status:MEDLINE
[do] DOI:10.1186/s13000-014-0195-7


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[PMID]:25276808
[Au] Autor:Benni S; Avramoglou T; Hlawaty H; Mora L
[Ad] Endereço:INSERM, U1148, LVTS, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 93430 Villetaneuse, France.
[Ti] Título:Dynamic contact angle analysis of protein adsorption on polysaccharide multilayer's films for biomaterial reendothelialization.
[So] Source:Biomed Res Int;2014:679031, 2014.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Atherosclerosis is a major cardiovascular disease. One of the side effects is restenosis. The aim of this work was to study the coating of stents by dextran derivates based polyelectrolyte's multilayer (PEM) films in order to increase endothelialization of injured arterial wall after stent implantation. Films were composed with diethylaminoethyl dextran (DEAE) as polycation and dextran sulphate (DS) as polyanion. One film was composed with 4 bilayers of (DEAE-DS)4 and was labeled D-. The other film was the same as D- but with an added terminal layer of DEAE polycation: (DEAE-DS)4-DEAE (labeled D+). The dynamic adsorption/desorption of proteins on the films were characterized by dynamic contact angle (DCA) and atomic force microscopy (AFM). Human endothelial cell (HUVEC) adhesion and proliferation were quantified and correlated to protein adsorption analyzed by DCA for fibronectin, vitronectin, and bovine serum albumin (BSA). Our results showed that the endothelial cell response was optimal for films composed of DS as external layer. Fibronectin was found to be the only protein to exhibit a reversible change in conformation after desorption test. This behavior was only observed for (DEAE-DS)4 films. (DEAE-DS)4 films could enhance HUVEC proliferation in agreement with fibronectin ability to easily change from conformation.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/farmacologia
Células Endoteliais da Veia Umbilical Humana/citologia
Polissacarídeos/química
Proteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Adsorção
Animais
Bovinos
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
DEAE-Dextrano/química
Sulfato de Dextrana/química
Fibronectinas/isolamento & purificação
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Microscopia de Força Atômica
Microscopia de Fluorescência
Soroalbumina Bovina/isolamento & purificação
Tensão Superficial/efeitos dos fármacos
Vitronectina/isolamento & purificação
Molhabilidade/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Fibronectins); 0 (Polysaccharides); 0 (Proteins); 0 (Vitronectin); 27432CM55Q (Serum Albumin, Bovine); 9015-73-0 (DEAE-Dextran); 9042-14-2 (Dextran Sulfate)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141003
[St] Status:MEDLINE
[do] DOI:10.1155/2014/679031



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