Base de dados : MEDLINE
Pesquisa : D09.698.550 [Categoria DeCS]
Referências encontradas : 4738 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 474 ir para página                         

  1 / 4738 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28450249
[Au] Autor:Kurt A; Toker OS; Tornuk F
[Ad] Endereço:Department of Food Engineering, Engineering Faculty, Ondokuz Mayis University, 55139, Samsun, Turkey; Department of Food Engineering, Engineering and Architecture Faculty, Bitlis Eren University, 13000 Bitlis, Turkey. Electronic address: abdullahkurt48@gmail.com.
[Ti] Título:Effect of xanthan and locust bean gum synergistic interaction on characteristics of biodegradable edible film.
[So] Source:Int J Biol Macromol;102:1035-1044, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study was aimed to use different combinations of xanthan (XG) and locust bean gum (LBG) in the biodegradable edible film preparation by benefitting from their synergistic interactions for the first time. Concentrations of LBG, XG and glycerol of the optimized film sample were found to be 89.6%, 10.4% and 20%, respectively. At the optimum point the WVP, TS, E% and EM values of film were found 0.22gmmh m kPa, 86.97MPa, 33.34% and 177.25MPa, respectively. The optimized film was characterized for its physical, thermal and structural behavior. The scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FTIR) analyses exhibited miscibility and presence of interaction between polymers. In conclusion, XG and LBG interaction was used successfully to get biodegradable films and coatings with improved characteristics.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Galactanos/química
Mananas/química
Gomas Vegetais/química
Polissacarídeos Bacterianos/química
Embalagem de Produtos
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/metabolismo
Composição de Medicamentos
Glicerol/química
Fenômenos Mecânicos
Permeabilidade
Reologia
Vapor
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides, Bacterial); 0 (Steam); PDC6A3C0OX (Glycerol); TTV12P4NEE (xanthan gum); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  2 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29361938
[Au] Autor:Maia-Landim A; Ramírez JM; Lancho C; Poblador MS; Lancho JL
[Ad] Endereço:Department of Morphological Sciences, School of Medicine, University of Córdoba, Avenida de Menéndez Pidal s/n, 14071, Córdoba, Spain.
[Ti] Título:Long-term effects of Garcinia cambogia/Glucomannan on weight loss in people with obesity, PLIN4, FTO and Trp64Arg polymorphisms.
[So] Source:BMC Complement Altern Med;18(1):26, 2018 Jan 24.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Overweight and obesity are considered major health problems that contribute to increase mortality and quality of life. Both conditions have a high prevalence across the world reaching epidemic numbers. Our aim was to evaluate the effects of the administration of Garcinia cambogia (GC) and Glucomannan (GNN) on long-term weight loss in people with overweight or obesity. METHODS: Prospective, not-randomized controlled intervention trial was conducted. We treated 214 subjects with overweight or obesity with GC and GNN (500 mg twice a day, each) for 6 months evaluating weight, fat mass, visceral fat, basal metabolic rate, and lipid and glucose blood profiles comparing them with basal values. Some patients were carriers of polymorphisms PLIN4 -11482G > A-, fat mass and obesity-associated (FTO) -rs9939609 A/T- and ß-adrenergic receptor 3 (ADRB3) -Trp64Arg. RESULTS: Treatment produced weight loss, reducing fat mass, visceral fat, lipid and blood glucose profiles while increasing basal metabolic rate. Results were independent of sex, age or suffering from hypertension, diabetes mellitus type 2 or dyslipidemia and were attenuated in carriers of PLIN4, FTO, Trp64Arg polymorphisms. CONCLUSIONS: Administration of GC and GNN reduce weight and improve lipid and glucose blood profiles in people with overweight or obesity, although the presence of polymorphisms PLIN4, FTO and ADRB3 might hinder in some degree these effects. ISRCTN78807585, 19 September 2017, retrospective study.
[Mh] Termos MeSH primário: Dioxigenase FTO Dependente de alfa-Cetoglutarato/genética
Garcinia cambogia
Mananas
Obesidade
Perilipina-4/genética
Receptores Adrenérgicos beta 3/genética
Perda de Peso
[Mh] Termos MeSH secundário: Adulto
Amorphophallus/química
Feminino
Seres Humanos
Masculino
Mananas/farmacologia
Mananas/uso terapêutico
Obesidade/tratamento farmacológico
Obesidade/epidemiologia
Obesidade/genética
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Polimorfismo Genético/genética
Estudos Prospectivos
Perda de Peso/efeitos dos fármacos
Perda de Peso/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ADRB3 protein, human); 0 (Mannans); 0 (PLIN4 protein, human); 0 (Perilipin-4); 0 (Plant Extracts); 0 (Receptors, Adrenergic, beta-3); 36W3E5TAMG ((1-6)-alpha-glucomannan); EC 1.14.11.33 (Alpha-Ketoglutarate-Dependent Dioxygenase FTO); EC 1.14.11.33 (FTO protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2099-7


  3 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460335
[Au] Autor:Feng Y; Li Q; Wu D; Niu Y; Yang C; Dong L; Wang C
[Ad] Endereço:State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China.
[Ti] Título:A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis.
[So] Source:Biomaterials;134:128-142, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Biomaterials scaffolds designed for many regenerative applications are expected to support neo-vascularisation, which is now being hampered by two limitations - the instability of exogenous growth factors (GFs) that are delivered to promote angiogenesis; and the loss of extracellular matrix components that bind and stabilise GFs. Here, we report the design and evaluation of an injectable hydrogel system aimed at restoring a GF-binding microenvironment to enhance the pro-angiogenic functions of endogenous GFs. This gel comprises two polysaccharides with their unique bioactivities: Konjac glucomannan (KGM) as the building block of the gel scaffold, for its demonstrated capacity to activate macrophages/monocytes to secrete pro-angiogenic/-mitogenic GFs; and heparin (Hep), a representative glycosaminoglycan molecule that binds numerous pro-angiogenic GFs, as functional moieties to sequester the macrophage-produced GFs. Modified with tyramine (TA) groups, the two polysaccharides can be co-polymerised and rapidly form into hydrogel upon enzyme catalysis. The designed KGM-TA/Hep-TA hydrogel successfully preserves the macrophage-activating function and GF-binding affinity of the two components, respectively, and, once subcutaneously implanted, effectively sequestered the locally-produced GFs in situ and promote the formation and maturation of blood vessels in mice. In summary, the designed hydrogel system demonstrates a feasible approach to stimulate the production and harness the function of endogenous GFs for inducing blood vessel formation in vivo, without the addition of any exogenous proteins. This design may provide an innovative, open platform to promote vascularisation for various regenerative purposes.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Hidrogel de Polietilenoglicol-Dimetacrilato/química
Hidrogel de Polietilenoglicol-Dimetacrilato/farmacologia
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Macrófagos/metabolismo
[Mh] Termos MeSH secundário: Indutores da Angiogênese/química
Indutores da Angiogênese/farmacologia
Animais
Glicosaminoglicanos/metabolismo
Seres Humanos
Integrina beta1/metabolismo
Lectinas Tipo C/metabolismo
Masculino
Mananas/metabolismo
Lectinas de Ligação a Manose/metabolismo
Camundongos
Neovascularização Fisiológica/efeitos dos fármacos
Polissacarídeos/metabolismo
Células RAW 264.7
Receptores de Superfície Celular/metabolismo
Células THP-1
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inducing Agents); 0 (Biocompatible Materials); 0 (Glycosaminoglycans); 0 (Integrin beta1); 0 (Intercellular Signaling Peptides and Proteins); 0 (Lectins, C-Type); 0 (Mannans); 0 (Mannose-Binding Lectins); 0 (Polysaccharides); 0 (Receptors, Cell Surface); 0 (mannose receptor); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); 36W3E5TAMG ((1-6)-alpha-glucomannan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  4 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29245249
[Au] Autor:Cho SY; Lee DG; Choi JK; Lee HJ; Kim SH; Park SH; Choi SM; Choi JH; Yoo JH; Park YJ; Lee JW
[Ad] Endereço:aDivision of Infectious Diseases, Department of Internal MedicinebVaccine Bio Research InstitutecThe Catholic Blood and Marrow Transplantation CentredDepartment of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
[Ti] Título:Characteristics of culture-positive invasive pulmonary aspergillosis in patients with hematologic diseases: Comparison between Aspergillus fumigatus and non-fumigatus Aspergillus species.
[So] Source:Medicine (Baltimore);96(49):e8841, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While the epidemiology and clinical differences of various Candida spp. has been relatively well-identified, data regarding invasive aspergillosis (IA) caused by different Aspergillus spp. are insufficient.We aimed to determine the epidemiology of culture-positive invasive pulmonary aspergillosis (IPA) and to compare the characteristics and outcomes of Aspergillus fumigatus IPA with those of non-fumigatus IPA in patients with hematologic diseases. All consecutive cases of IPA from 2011 to 2015 were reviewed retrospectively.There were 430 proven/probable IPA and 76 culture-positive proven/probable IPA. Excluding cases of multiple species of fungi or cases having difficulties in species-level identification, 41 A fumigatus and 22 non-fumigatus IPA (Aspergillus flavus [n = 11], Aspergillus niger [n = 6], and Aspergillus terreus [n = 5]) were compared. There were no significant differences in baseline characteristics between the 2 groups. However, disseminated IA was more common in non-fumigatus IPA (2.4% vs 18.2%; P = .046). Paranasal sinus (PNS) involvement was more common in non-fumigatus IPA. There was a trend towards higher peak serum galactomannan values in non-fumigatus IPA than in A fumigatus IPA group (median 1.33 [interquartile 0.98-3.29] vs 0.97 [0.66-1.97]; P = .084). Clinical response and mortality did not differ between groups.The culture-positive rate of proven/probable IPA was 17.7%, of which non-fumigatus Aspergillus accounted for about one-third. Disseminated IA, especially involving the PNS, was more frequent in non-fumigatus IPA than in A fumigatus IPA.
[Mh] Termos MeSH primário: Aspergillus fumigatus
Aspergillus
Doenças Hematológicas/microbiologia
Aspergilose Pulmonar Invasiva/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Doenças Hematológicas/sangue
Seres Humanos
Aspergilose Pulmonar Invasiva/sangue
Masculino
Mananas/sangue
Meia-Idade
Seios Paranasais/microbiologia
Estudos Retrospectivos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannans); 11078-30-1 (galactomannan)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008841


  5 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873574
[Au] Autor:Owens C; Griffin K; Khouryieh H; Williams K
[Ad] Endereço:Department of Chemistry, Western Kentucky University, Bowling Green, KY, USA.
[Ti] Título:Creaming and oxidative stability of fish oil-in-water emulsions stabilized by whey protein-xanthan-locust bean complexes: Impact of pH.
[So] Source:Food Chem;239:314-322, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impact of pH on the physicochemical properties of 10% menhaden oil-in-water (O/W) emulsions containing 2% whey protein isolate (WPI) and 0.1% xanthan (XG)-locust bean gum (LBG) mixtures was investigated. The O/W emulsions containing 0.1% XG-LBG mixtures were compared to emulsions with 0.1% XG and 0.1% LBG. The results indicated that stability is dependent on pH and biopolymer type. At both pH 3 and 5, emulsions containing either XG or XG-LBG mixtures had large particle sizes, viscosity, droplet aggregation, and creaming index, resulting in poor physical stability which can be related to the adsorbed protein-polysaccharide interactions. At pH7, the XG-LBG emulsions showed the greatest resistance to phase separation and resulted in stable emulsions. Lipid oxidation measurements also indicated that XG-LBG mixtures can be used to form stable emulsions at pH 3 and pH 7. These results have significant implications for the development of novel structures containing lipid phases susceptible to lipid oxidation.
[Mh] Termos MeSH primário: Óleos de Peixe/química
[Mh] Termos MeSH secundário: Animais
Emulsões
Galactanos
Gafanhotos
Concentração de Íons de Hidrogênio
Mananas
Gomas Vegetais
Polissacarídeos Bacterianos
Água
Proteínas do Soro do Leite
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Fish Oils); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides, Bacterial); 0 (Whey Proteins); 059QF0KO0R (Water); TTV12P4NEE (xanthan gum); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  6 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973595
[Au] Autor:Miceli MH; Kauffman CA
[Ad] Endereço:Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
[Ti] Título:Aspergillus Galactomannan for Diagnosing Invasive Aspergillosis.
[So] Source:JAMA;318(12):1175-1176, 2017 Sep 26.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Aspergillus fumigatus
Líquido da Lavagem Broncoalveolar/química
Hospedeiro Imunocomprometido
Técnicas Imunoenzimáticas
Aspergilose Pulmonar Invasiva/diagnóstico
Mananas/análise
[Mh] Termos MeSH secundário: Idoso
Aspergillus fumigatus/isolamento & purificação
Biópsia
Líquido da Lavagem Broncoalveolar/microbiologia
Granulomatose com Poliangiite/complicações
Granulomatose com Poliangiite/tratamento farmacológico
Seres Humanos
Masculino
Mananas/sangue
Sensibilidade e Especificidade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannans); 11078-30-1 (galactomannan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.10661


  7 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28855252
[Au] Autor:Rahlwes KC; Ha SA; Motooka D; Mayfield JA; Baumoel LR; Strickland JN; Torres-Ocampo AP; Nakamura S; Morita YS
[Ad] Endereço:From the Department of Microbiology, University of Massachusetts, Amherst, MA 01003.
[Ti] Título:The cell envelope-associated phospholipid-binding protein LmeA is required for mannan polymerization in mycobacteria.
[So] Source:J Biol Chem;292(42):17407-17417, 2017 Oct 20.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The integrity of the distinguishing, multilaminate cell envelope surrounding mycobacteria is critical to their survival and pathogenesis. The prevalence of phosphatidylinositol mannosides in the cell envelope suggests an important role in the mycobacterial life cycle. Indeed, deletion of the gene (Δ ) encoding the first committed step in phosphatidylinositol hexamannoside biosynthesis in results in the formation of smaller colonies than wild-type colonies on Middlebrook 7H10 agar. To further investigate potential contributors to cell-envelope mannan biosynthesis while taking advantage of this colony morphology defect, we isolated spontaneous suppressor mutants of Δ that reverted to wild-type colony size. Of 22 suppressor mutants, 6 accumulated significantly shorter lipomannan or lipoarabinomannan. Genome sequencing of these mutants revealed mutations in genes involved in the lipomannan/lipoarabinomannan biosynthesis, such as those encoding the arabinosyltransferase EmbC and the mannosyltransferase MptA. Furthermore, we identified three mutants carrying a mutation in a previously uncharacterized gene, _ , that we designated Complementation of these suppressor mutants with restored the original Δ phenotypes and deletion of in wild-type resulted in smaller lipomannan, as observed in the suppressor mutants. LmeA carries a predicted N-terminal signal peptide, and density gradient fractionation and detergent extractability experiments indicated that LmeA localizes to the cell envelope. Using a lipid ELISA, we found that LmeA binds to plasma membrane phospholipids, such as phosphatidylethanolamine and phosphatidylinositol. LmeA is widespread throughout the Corynebacteriales; therefore, we concluded that LmeA is an evolutionarily conserved cell-envelope protein critical for controlling the mannan chain length of lipomannan/lipoarabinomannan.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Membrana Celular/metabolismo
Mananas/biossíntese
Manosiltransferases/metabolismo
Mycobacterium smegmatis/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Membrana Celular/genética
Lipopolissacarídeos/biossíntese
Lipopolissacarídeos/genética
Mananas/genética
Manosiltransferases/genética
Mycobacterium smegmatis/genética
Fosfolipídeos/genética
Fosfolipídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lipopolysaccharides); 0 (Mannans); 0 (Phospholipids); 0 (lipoarabinomannan); 0 (lipomannan); EC 2.4.1.- (Mannosyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.804377


  8 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28846383
[Au] Autor:Gerlits OO; Coates L; Woods RJ; Kovalevsky A
[Ad] Endereço:UT/ORNL Joint Institute for Biological Sciences, University of Tennessee , Knoxville, Tennessee 37996, United States.
[Ti] Título:Mannobiose Binding Induces Changes in Hydrogen Bonding and Protonation States of Acidic Residues in Concanavalin A As Revealed by Neutron Crystallography.
[So] Source:Biochemistry;56(36):4747-4750, 2017 Sep 12.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant lectins are carbohydrate-binding proteins with various biomedical applications. Concanavalin A (Con A) holds promise in treating cancerous tumors. To better understand the Con A carbohydrate binding specificity, we obtained a room-temperature neutron structure of this legume lectin in complex with a disaccharide Manα1-2Man, mannobiose. The neutron structure afforded direct visualization of the hydrogen bonding between the protein and ligand, showing that the ligand is able to alter both protonation states and interactions for residues located close to and distant from the binding site. An unprecedented low-barrier hydrogen bond was observed forming between the carboxylic side chains of Asp28 and Glu8, with the D atom positioned equidistant from the oxygen atoms having an O···D···O angle of 101.5°.
[Mh] Termos MeSH primário: Concanavalina A/química
Concanavalina A/metabolismo
Mananas/química
Mananas/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação
Ligações de Hidrogênio
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannans); 11028-71-0 (Concanavalin A); 15548-43-3 (mannobiose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00654


  9 / 4738 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28771901
[Au] Autor:Maiti K; Syal K; Chatterji D; Jayaraman N
[Ad] Endereço:Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.
[Ti] Título:Synthetic Arabinomannan Heptasaccharide Glycolipids Inhibit Biofilm Growth and Augment Isoniazid Effects in Mycobacterium smegmatis.
[So] Source:Chembiochem;18(19):1959-1970, 2017 10 05.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Biofilm formation, involving attachment to an adherent surface, is a critical survival strategy of mycobacterial colonies in hostile environmental conditions. Here we report the synthesis of heptasaccharide glycolipids based on mannopyranoside units anchored on to a branched arabinofuranoside core. Two types of glycolipids-2,3-branched and 2,5-branched-were synthesized and evaluated for their efficacies in inhibiting biofilm growth by the non-pathogenic mycobacterium variant Mycobacterium smegmatis. Biofilm formation was inhibited at a minimum biofilm growth inhibition concentration (MBIC) of 100 µg mL in the case of the 2,5-branched heptasaccharide glycolipid. Further, we were able to ascertain that a combination of the drug isoniazid with the branched heptasaccharide glycolipid (50 µg mL ) potentiates the drug, making it three times more effective, with an improved MBIC of 30 µg mL . These studies establish that synthetic glycolipids not only act as inhibitors of biofilm growth, but also provide a synergistic effect when combined with significantly lowered concentrations of isoniazid to disrupt the biofilm structures of the mycobacteria.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Glicolipídeos/farmacologia
Isoniazida/farmacologia
Mananas/química
Mycobacterium smegmatis/efeitos dos fármacos
Oligossacarídeos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Relação Dose-Resposta a Droga
Glicolipídeos/síntese química
Glicolipídeos/química
Isoniazida/química
Testes de Sensibilidade Microbiana
Estrutura Molecular
Mycobacterium smegmatis/metabolismo
Oligossacarídeos/síntese química
Oligossacarídeos/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Glycolipids); 0 (Mannans); 0 (Oligosaccharides); 53026-40-7 (arabinomannan); V83O1VOZ8L (Isoniazid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700247


  10 / 4738 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28732013
[Au] Autor:Luo M; Yang S; Li X; Liu P; Xue J; Zhou X; Su K; Xu X; Qing Y; Qiu J; Li Y
[Ad] Endereço:School of Public Health and Management, Chongqing Medical University, Chongqing, China.
[Ti] Título:The KP1_4563 gene is regulated by the cAMP receptor protein and controls type 3 fimbrial function in Klebsiella pneumoniae NTUH-K2044.
[So] Source:PLoS One;12(7):e0180666, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen that can adhere to host cells or extracellular matrix via type 1 and type 3 fimbriae. KP1_4563 is a gene encoding a hypothetical protein in K. pneumoniae NTUH-K2044. KP1_4563 is located between the type 1 and type 3 fimbrial gene clusters and is likely associated with fimbrial function given its putative conserved domains of unknown function (DUF1471). Cyclic AMP receptor protein (CRP) regulates virulence-related gene expression and is a crucial transcriptional regulator in many bacteria. The predicted DNA recognition motif of CRP is present in the KP1_4563 promoter region. This study aimed to investigate the function of KP1_4563 in fimbriae and its transcriptional regulation mechanism by CRP. We generated Kp-Δ4563 mutant and complementation strains. We utilized phenotype and adhesion assays to evaluate the role of KP1_4563 in fimbriae. We conducted quantitative RT-PCR (qRT-PCR), LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays to study the transcriptional regulation of KP1_4563 gene by CRP. We found that KP1_4563 negatively regulates the function of type 3 fimbriae. Compared with NTUH-K2044, the absence of KP1_4563 enhanced the ability of Kp-Δ4563 to adhere to A549 cells. CRP negatively regulates KP1_4563 by directly binding to its promoter region. KP1_4563 plays an important role in type 3 fimbrial function. This novel insight will assist in the development of strategies for preventing K. pneumoniae infection.
[Mh] Termos MeSH primário: Proteína Receptora de AMP Cíclico/metabolismo
Proteínas de Fímbrias/metabolismo
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
[Mh] Termos MeSH secundário: Aderência Bacteriana/fisiologia
Pegada de DNA
Desoxirribonuclease I/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Escherichia coli
Regulação da Expressão Gênica/fisiologia
Testes de Hemaglutinação
Óperon Lac
Mananas/química
Fenótipo
Reação em Cadeia da Polimerase em Tempo Real
Saccharomyces cerevisiae
Deleção de Sequência
Transcrição Genética/fisiologia
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclic AMP Receptor Protein); 0 (Mannans); 147680-16-8 (Fimbriae Proteins); EC 3.1.21.1 (Deoxyribonuclease I); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180666



página 1 de 474 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde