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Pesquisa : D09.698.629 [Categoria DeCS]
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  1 / 22230 MEDLINE  
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[PMID]:29200854
[Au] Autor:Qu Y; Wang Z; Zhou H; Kang M; Dong R; Zhao J
[Ad] Endereço:Department of Orthopedics, The Second Hospital of Jilin University, Changchun, People's Republic of China.
[Ti] Título:Oligosaccharide nanomedicine of alginate sodium improves therapeutic results of posterior lumbar interbody fusion with cages for degenerative lumbar disease in osteoporosis patients by downregulating serum miR-155.
[So] Source:Int J Nanomedicine;12:8459-8469, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Degenerative lumbar disease (DLD) is a significant issue for public health. Posterior lumbar intervertebral fusion with cages (PLIFC) has high-level fusion rate and realignment on DLD. However, there are some complications following the surgery. Alginate oligosaccharides (AOS) have antioxidant and anti-inflammatory activities and may be suitable for infection therapy. MiR-155 is a biomarker associated with inflammatory and oxidative stress. AOS may promote PLIFC therapy by regulating miR-155. Pluronic nanoparticles and oligosaccharide nanomedicine of alginate sodium (ONAS) were prepared with ampicillin at size <200 nm. Ninety-six DLD osteoporosis patients received PLIFC and were evenly assigned into ONAS group (OG, oral administration of 100 mg ONAS daily) and control group (PG, 100 mg pluronic nanoparticles). Serum miR-155 level was measured by real-time quantitative PCR. The levels of superoxide dismutase (SOD), glutathione (GSH), aspartate aminotransaminase (AST), alanine aminotransferase (ALT), interleukin-1ß (IL-1ß), and interleukin-1 receptor antagonist (IL-1ra) were measured. Weighted mean difference (WMD), relative risk (RR), complications, surgery infection rate, fusion rate, and Japanese Orthopaedic Association (JOA) scores were used to evaluate therapeutic efficacy. After 1-month therapy, infection rates and side effects were lower in OG than those in PG (RR =0.64, 95% confidence interval [CI] [0.48, 0.84], =0.001). The fusion rates were higher in OG than in PG (WMD =21.96, 95% CI [-0.24, 37.62], =0.021). The JOA scores were higher in OG than in PG (RR =0.52, 95% CI [0.33, 0.84], =0.007), and no significant difference was found for the visual analog scale and Oswestry Disability Index. Serum levels of miR-155, ALT, AST, and IL-1ß were lower while SOD, GSH, and IL-1ra were higher in OG than in PG. MiR-155 mimic increased the levels of ALT, AST, and IL-1ß and reduced the levels of SOD, GSH, and IL-1ra. In contrast, miR-155 inhibitor had reverse results. Therefore, ONAS has better improvement in complications and therapeutic effects on DLD by regulating serum miR-155.
[Mh] Termos MeSH primário: Alginatos/farmacologia
Degeneração do Disco Intervertebral/terapia
Vértebras Lombares/patologia
MicroRNAs/sangue
Nanomedicina/métodos
Oligossacarídeos/farmacologia
Osteoporose/complicações
Fusão Vertebral
[Mh] Termos MeSH secundário: Idoso
Antioxidantes/farmacologia
Linhagem Celular Tumoral
Citocinas/metabolismo
Regulação para Baixo/efeitos dos fármacos
Regulação para Baixo/genética
Feminino
Ácido Glucurônico/farmacologia
Ácidos Hexurônicos/farmacologia
Seres Humanos
Degeneração do Disco Intervertebral/sangue
Degeneração do Disco Intervertebral/genética
Masculino
MicroRNAs/genética
Nanopartículas/ultraestrutura
Estresse Oxidativo/efeitos dos fármacos
Garantia da Qualidade dos Cuidados de Saúde
Resultado do Tratamento
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Antioxidants); 0 (Cytokines); 0 (Hexuronic Acids); 0 (MIRN155 microRNA, human); 0 (MicroRNAs); 0 (Oligosaccharides); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S143824


  2 / 22230 MEDLINE  
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[PMID]:28465178
[Au] Autor:Das S; Ghosh S; De AK; Bera T
[Ad] Endereço:Laboratory of Nanomedicine, Division of Pharmaceutical Biotechnology, Department of Pharmaceutical Technology, Jadavpur University, 188 Raja S.C. Mallick Road, Kolkata, 700 032, W.B., India.
[Ti] Título:Oral delivery of ursolic acid-loaded nanostructured lipid carrier coated with chitosan oligosaccharides: Development, characterization, in vitro and in vivo assessment for the therapy of leishmaniasis.
[So] Source:Int J Biol Macromol;102:996-1008, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Visceral leishmaniasis (VL) is a life-threatening disease caused by Leishmania donovani due to uncontrolled parasitisation of liver, spleen, and bone marrow. Ursolic acid (UA), a promising anti-inflammatory, anti-bacterial and anti-diabetic drug used successfully for treatment of ailments. Development of new delivery system is extremely urgent for UA with better efficacy and fewer side effects. The aim of present research work was to formulate and evaluate the potential anti-leishmanial activity of UA loaded N-octyl-chitosan surface decorated nanostructured lipid carrier system (UA-NLC) for delivery to the macrophages for VL. UA-NLC were prepared and characterized for shape, size, fourier transforms scanning electron microscopy (FESEM), transmittance electron microscopy (TEM), entrapment efficiency and in vitro drug release. The results indicate that the formulated UA-NLC had nano size range (103.7±2.8nm to 143.0±3.8nm) with high drug loading capacity (12.05±0.54%) and entrapment efficiency (88.63±2.7%). Ex vivo drug uptake by macrophage was also evaluated. The UA-NLC was more effective against AG83 wild type (12 fold), SSG-R (4 fold), PMM-R (4 fold) and GE1 field isolated (3 fold) cellular amastigotes than its free form. In vivo study showed orally effective UA-NLC could suppress the parasite burden to 98.75%.
[Mh] Termos MeSH primário: Quitosana/química
Portadores de Fármacos/química
Leishmaniose/tratamento farmacológico
Lipídeos/química
Nanoestruturas/química
Triterpenos/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Desenho de Drogas
Liberação Controlada de Fármacos
Feminino
Macrófagos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Oligossacarídeos/química
Triterpenos/administração & dosagem
Triterpenos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Lipids); 0 (Oligosaccharides); 0 (Triterpenes); 9012-76-4 (Chitosan); P3M2575F3F (ursolic acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 22230 MEDLINE  
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[PMID]:28465177
[Au] Autor:Liu NN; Chi Z; Wang QQ; Hong J; Liu GL; Hu Z; Chi ZM
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
[Ti] Título:Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
[So] Source:Int J Biol Macromol;102:1016-1024, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×10 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×10 and a pullulan titer was 65.3g/L. The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase. During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×10 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×10 . Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5. Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
[Mh] Termos MeSH primário: Ascomicetos/isolamento & purificação
Ascomicetos/metabolismo
Glucanos/biossíntese
Glucanos/química
Oligossacarídeos/biossíntese
Oligossacarídeos/química
Zonas Úmidas
[Mh] Termos MeSH secundário: Fermentação
Peso Molecular
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Oligosaccharides); 8ZQ0AYU1TT (pullulan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  4 / 22230 MEDLINE  
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[PMID]:29177276
[Au] Autor:Dallabernardina P; Ruprecht C; Smith PJ; Hahn MG; Urbanowicz BR; Pfrengle F
[Ad] Endereço:Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany. Fabian.Pfrengle@mpikg.mpg.de.
[Ti] Título:Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies.
[So] Source:Org Biomol Chem;15(47):9996-10000, 2017 Dec 06.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).
[Mh] Termos MeSH primário: Anticorpos Monoclonais/química
Arabidopsis/química
Automação
Parede Celular/química
Oligossacarídeos/síntese química
Polissacarídeos/química
[Mh] Termos MeSH secundário: Arabidopsis/enzimologia
Parede Celular/enzimologia
Análise em Microsséries
Oligossacarídeos/química
Oligossacarídeos/metabolismo
Pentosiltransferases/metabolismo
Polissacarídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Oligosaccharides); 0 (Polysaccharides); EC 2.4.2.- (Pentosyltransferases); EC 2.4.2.- (xyloglucan xylosyltransferase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob02605f


  5 / 22230 MEDLINE  
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[PMID]:29335469
[Au] Autor:Aunkham A; Zahn M; Kesireddy A; Pothula KR; Schulte A; Baslé A; Kleinekathöfer U; Suginta W; van den Berg B
[Ad] Endereço:Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
[Ti] Título:Structural basis for chitin acquisition by marine Vibrio species.
[So] Source:Nat Commun;9(1):220, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chitin, an insoluble polymer of N-acetylglucosamine, is one of the most abundant biopolymers on Earth. By degrading chitin, chitinolytic bacteria such as Vibrio harveyi are critical for chitin recycling and maintenance of carbon and nitrogen cycles in the world's oceans. A decisive step in chitin degradation is the uptake of chito-oligosaccharides by an outer membrane protein channel named chitoporin (ChiP). Here, we report X-ray crystal structures of ChiP from V. harveyi in the presence and absence of chito-oligosaccharides. Structures without bound sugar reveal a trimeric assembly with an unprecedented closing of the transport pore by the N-terminus of a neighboring subunit. Substrate binding ejects the pore plug to open the transport channel. Together with molecular dynamics simulations, electrophysiology and in vitro transport assays our data provide an explanation for the exceptional affinity of ChiP for chito-oligosaccharides and point to an important role of the N-terminal gate in substrate transport.
[Mh] Termos MeSH primário: Carbono/metabolismo
Quitina/metabolismo
Nitrogênio/metabolismo
Vibrio/metabolismo
[Mh] Termos MeSH secundário: Acetilglucosamina/metabolismo
Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/metabolismo
Ciclo do Carbono
Cristalografia por Raios X
Modelos Moleculares
Ciclo do Nitrogênio
Oceanos e Mares
Oligossacarídeos/metabolismo
Porinas/química
Porinas/genética
Porinas/metabolismo
Conformação Proteica
Água do Mar/química
Água do Mar/microbiologia
Vibrio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Oligosaccharides); 0 (Porins); 1398-61-4 (Chitin); 7440-44-0 (Carbon); N762921K75 (Nitrogen); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02523-y


  6 / 22230 MEDLINE  
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[PMID]:29184406
[Au] Autor:Yuan M; Ding S; Meng T; Lu B; Shao S; Zhang X; Yuan H; Hu F
[Ad] Endereço:Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan.
[Ti] Título:Effect of A-317491 delivered by glycolipid-like polymer micelles on endometriosis pain.
[So] Source:Int J Nanomedicine;12:8171-8183, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Endometriosis is a common gynecological disease with a lack of effective clinical treatment. Current therapy often results in endometriosis pain recurrence and serious side effects. P2X receptor, an adenosine triphosphate (ATP)-gated ion channel, might be implicated in endometriosis pain. In this study, chitosan oligosaccharide-g-stearic acid (CSOSA) polymer micelles-coated nanostructured lipid carriers (NLCs) were developed as a novel delivery system for A-317491, a selective P2X receptor antagonist for endometriosis pain therapy. A-317491-loaded NLC (NLC/A-317491) could be coated by CSOSA micelles to form CSOSA/NLC/A-317491 nanoparticles. Pheochromocytoma PC12 cells, which highly expressed P2X receptors, were used as a cell model, and the CSOSA/NLC/A-317491 partly blocked the Ca influx induced by ATP stimulation. In nude mouse and rat endometriotic models, CSOSA/NLC could accumulate into endometriotic lesions after vein injection. In endometriotic rats, CSOSA/NLC/A-317491 reversed mechanical and heat hyperalgesia with long-term efficacy, which might be attributed to the massive CSOSA/NLC/A-317491 distribution in the endometriotic lesions. In conclusion, A-317491 delivered by CSOSA/NLC nanoparticles attenuated endometriosis pain in rats, and CSOSA/NLC/A-317491 could be used as an effective treatment strategy for P2X -targeted therapy in endometriosis pain.
[Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos/métodos
Endometriose/tratamento farmacológico
Nanopartículas/administração & dosagem
Dor/tratamento farmacológico
Fenóis/administração & dosagem
Compostos Policíclicos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Feminino
Glicolipídeos/química
Seres Humanos
Camundongos Nus
Micelas
Nanopartículas/química
Oligossacarídeos/química
Células PC12
Fenóis/química
Fenóis/farmacologia
Compostos Policíclicos/química
Compostos Policíclicos/farmacologia
Polímeros/química
Antagonistas do Receptor Purinérgico P2X/farmacologia
Ratos
Receptores Purinérgicos P2X3
Ácidos Esteáricos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A-317491); 0 (Glycolipids); 0 (Micelles); 0 (Oligosaccharides); 0 (Phenols); 0 (Polycyclic Compounds); 0 (Polymers); 0 (Purinergic P2X Receptor Antagonists); 0 (Receptors, Purinergic P2X3); 0 (Stearic Acids); 4ELV7Z65AP (stearic acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146569


  7 / 22230 MEDLINE  
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[PMID]:29363966
[Au] Autor:Van den Abbeele P; Taminiau B; Pinheiro I; Duysburgh C; Jacobs H; Pijls L; Marzorati M
[Ad] Endereço:ProDigest bvba , Technologiepark 3, 9052 Ghent, Belgium.
[Ti] Título:Arabinoxylo-Oligosaccharides and Inulin Impact Inter-Individual Variation on Microbial Metabolism and Composition, Which Immunomodulates Human Cells.
[So] Source:J Agric Food Chem;66(5):1121-1130, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fecal batch fermentations coupled to cocultures of epithelial cells and macrophages were used to compare how arabinoxylo-oligosaccharides (AXOS) and inulin modulate gut microbial activity and composition of three different human donors and subsequently the epithelial permeability and immune response. Both inulin and AXOS decreased the pH during incubation (-1.5 pH units), leading to increased productions of acetate, propionate, and butyrate. Differences in terms of metabolites production could be linked to specific microbial alterations at genus level upon inulin/AXOS supplementation (i.e., Bifidobacterium, Bacteroides, Prevotella and unclassified Erysipelotrichaceae), as shown by 16S-targeted Illumina sequencing. Both products stimulated gut barrier and immune function with increases in TEER, NF-KB, IL-10, and IL-6. Ingredients with different structures selectively modulate the microbiota of a specific donor leading to differential changes at metabolic level. The extent of this effect is donor specific and is linked to a final specific modulation of the host's immune system.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal/efeitos dos fármacos
Imunomodulação/efeitos dos fármacos
Inulina/farmacologia
Oligossacarídeos/farmacologia
Xilanos/farmacologia
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Butiratos/metabolismo
Células CACO-2
Fezes/microbiologia
Fermentação
Microbioma Gastrointestinal/imunologia
Microbioma Gastrointestinal/fisiologia
Seres Humanos
Concentração de Íons de Hidrogênio
Propionatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Butyrates); 0 (Oligosaccharides); 0 (Propionates); 0 (Xylans); 9005-80-5 (Inulin); 9040-27-1 (arabinoxylan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04611


  8 / 22230 MEDLINE  
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[PMID]:29363955
[Au] Autor:Oh SY; Youn SY; Park MS; Baek NI; Ji GE
[Ad] Endereço:Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University , Seoul 151-742, Republic of Korea.
[Ti] Título:Synthesis of Stachyobifiose Using Bifidobacterial α-Galactosidase Purified from Recombinant Escherichia coli.
[So] Source:J Agric Food Chem;66(5):1184-1190, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The prebiotic effects of GOS (galactooligosaccharides) are known to depend on the glycosidic linkages, degree of polymerization (DP), and the monosaccharide composition. In this study, a novel form of α-GOS with a potentially improved prebiotic effect was synthesized using bifidobacterial α-galactosidase (α-Gal) purified from recombinant Escherichia coli. The carbohydrate produced was identified as α-d-galactopyranosyl-(1→6)-O-α-d-glucopyranosyl-(1→2)-[α-d-galactopyranosyl-(1→6)-O-ß-d-fructofuranoside] and was termed stachyobifiose. Among 17 nonprobiotics, 16 nonprobiotics showed lower growth on stachyobifiose than ß-GOS. In contrast, among the 16 probiotics, 6 probiotics showed higher growth on stachyobifiose than ß-GOS. When compared with raffinose, stachyobifiose was used less by nonprobiotics than raffinose. Moreover, compared with stachyose, stachyobifiose was used less by Escherichia coli, Enterobacter cloacae, and Clostridium butyricum. The average amounts of total short-chain fatty acids (SCFA) produced were in the order of stachyobifiose > stachyose > raffinose > ß-GOS. Taken together, stachyobifiose is expected to contribute to beneficial changes of gut microbiota.
[Mh] Termos MeSH primário: Bifidobacterium/enzimologia
Microbioma Gastrointestinal/fisiologia
Oligossacarídeos/biossíntese
Prebióticos
Proteínas Recombinantes/metabolismo
alfa-Galactosidase/metabolismo
[Mh] Termos MeSH secundário: Bactérias/crescimento & desenvolvimento
Escherichia coli/enzimologia
Escherichia coli/genética
Galactose/metabolismo
Oligossacarídeos/metabolismo
Probióticos
Rafinose/metabolismo
alfa-Galactosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Prebiotics); 0 (Recombinant Proteins); 25VX64653N (stachyose); EC 3.2.1.22 (alpha-Galactosidase); N5O3QU595M (Raffinose); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04703


  9 / 22230 MEDLINE  
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[PMID]:29334221
[Au] Autor:Liu L; Gong W; Sun X; Chen G; Wang L
[Ad] Endereço:State Key Laboratory of Microbial Technology, Shandong University , 27 Shandanan Road, Jinan 250100, China.
[Ti] Título:Extracellular Enzyme Composition and Functional Characteristics of Aspergillus niger An-76 Induced by Food Processing Byproducts and Based on Integrated Functional Omics.
[So] Source:J Agric Food Chem;66(5):1285-1295, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails. In this study, we utilized integrated functional omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts. The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger. Compared with other substrates, wheat bran was more effective at inducing the secretion of ß-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases. Compared with proteins induced by single wheat bran or soybean dregs, the protein yield induced by their mixture was doubled, and the time required to reach peak enzyme activity was shortened by 25%. This study provided a technical platform for the complex formulation of various substrates and functional analysis of extracellular enzymes.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Aspergillus niger/crescimento & desenvolvimento
Indução Enzimática/efeitos dos fármacos
Manipulação de Alimentos
Oligossacarídeos/farmacologia
Resíduos
[Mh] Termos MeSH secundário: Arabinose/farmacologia
Ácido Aspártico Proteases/biossíntese
Celulases/biossíntese
Fibras na Dieta/análise
Grãos Comestíveis/química
Fermentação
Glucose/farmacologia
Glicosídeo Hidrolases/biossíntese
Peptídeo Hidrolases/biossíntese
Xilose/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 0 (Oligosaccharides); 0 (Waste Products); A1TA934AKO (Xylose); B40ROO395Z (Arabinose); EC 3.2.1.- (Cellulases); EC 3.2.1.- (Glycoside Hydrolases); EC 3.4.- (Aspartic Acid Proteases); EC 3.4.- (Peptide Hydrolases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180116
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05164


  10 / 22230 MEDLINE  
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[PMID]:28456975
[Au] Autor:Tokhtaeva E; Mareninova OA; Gukovskaya AS; Vagin O
[Ad] Endereço:David Geffen School of Medicine, University of California at Los Angeles, 10833 Le Conte Ave, Los Angeles, 90095, CA, USA.
[Ti] Título:Analysis of N- and O-Glycosylation of Lysosomal Glycoproteins.
[So] Source:Methods Mol Biol;1594:35-42, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The vast majority of lysosomal proteins are heavily glycosylated. The present protocol describes the method of analyzing N- and O-linked glycans in lysosomal proteins of interest. The method is based on using deglycosylating enzymes, endoglycosidases, and exoglycosidases. Endoglycosidases catalyze the cleavage of an internal bond in an oligosaccharide, while exoglycosidases remove terminal carbohydrates from glycans. Different types of carbohydrate residues or chains can be removed by specific glycosidases. Removing oligosaccharides with glycosidases increases the electrophoretic mobility of a protein. This increase in mobility depends on the size and number of removed carbohydrate chains. Therefore, the treatment of lysosomal proteins with specific glycosidases followed by a western blot analysis of a protein of interest provides a way to determine which types of glycans are present in the protein by comparing the gel mobility before and after treatment.
[Mh] Termos MeSH primário: Glicoproteínas/análise
Polissacarídeos/análise
Proteínas/análise
[Mh] Termos MeSH secundário: Western Blotting
Glicoproteínas/metabolismo
Glicosilação
Oligossacarídeos/análise
Oligossacarídeos/metabolismo
Polissacarídeos/metabolismo
Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Oligosaccharides); 0 (Polysaccharides); 0 (Proteins); 0 (lysosomal proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6934-0_3



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