Base de dados : MEDLINE
Pesquisa : D09.698.629.802.700 [Categoria DeCS]
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[PMID]:29363955
[Au] Autor:Oh SY; Youn SY; Park MS; Baek NI; Ji GE
[Ad] Endereço:Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University , Seoul 151-742, Republic of Korea.
[Ti] Título:Synthesis of Stachyobifiose Using Bifidobacterial α-Galactosidase Purified from Recombinant Escherichia coli.
[So] Source:J Agric Food Chem;66(5):1184-1190, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The prebiotic effects of GOS (galactooligosaccharides) are known to depend on the glycosidic linkages, degree of polymerization (DP), and the monosaccharide composition. In this study, a novel form of α-GOS with a potentially improved prebiotic effect was synthesized using bifidobacterial α-galactosidase (α-Gal) purified from recombinant Escherichia coli. The carbohydrate produced was identified as α-d-galactopyranosyl-(1→6)-O-α-d-glucopyranosyl-(1→2)-[α-d-galactopyranosyl-(1→6)-O-ß-d-fructofuranoside] and was termed stachyobifiose. Among 17 nonprobiotics, 16 nonprobiotics showed lower growth on stachyobifiose than ß-GOS. In contrast, among the 16 probiotics, 6 probiotics showed higher growth on stachyobifiose than ß-GOS. When compared with raffinose, stachyobifiose was used less by nonprobiotics than raffinose. Moreover, compared with stachyose, stachyobifiose was used less by Escherichia coli, Enterobacter cloacae, and Clostridium butyricum. The average amounts of total short-chain fatty acids (SCFA) produced were in the order of stachyobifiose > stachyose > raffinose > ß-GOS. Taken together, stachyobifiose is expected to contribute to beneficial changes of gut microbiota.
[Mh] Termos MeSH primário: Bifidobacterium/enzimologia
Microbioma Gastrointestinal/fisiologia
Oligossacarídeos/biossíntese
Prebióticos
Proteínas Recombinantes/metabolismo
alfa-Galactosidase/metabolismo
[Mh] Termos MeSH secundário: Bactérias/crescimento & desenvolvimento
Escherichia coli/enzimologia
Escherichia coli/genética
Galactose/metabolismo
Oligossacarídeos/metabolismo
Probióticos
Rafinose/metabolismo
alfa-Galactosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Prebiotics); 0 (Recombinant Proteins); 25VX64653N (stachyose); EC 3.2.1.22 (alpha-Galactosidase); N5O3QU595M (Raffinose); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04703


  2 / 2498 MEDLINE  
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[PMID]:29304068
[Au] Autor:Drake AC; Lee Y; Burgess EM; Karlsson JOM; Eroglu A; Higgins AZ
[Ad] Endereço:School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
[So] Source:PLoS One;13(1):e0190713, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.
[Mh] Termos MeSH primário: Crioprotetores/química
Polímeros/química
Açúcares/química
Temperatura de Transição
Água/química
[Mh] Termos MeSH secundário: Tampões (Química)
Varredura Diferencial de Calorimetria
Criopreservação/métodos
Dessecação/métodos
Dimetil Sulfóxido/química
Etilenoglicol/química
Ficoll/química
Vidro/química
Modelos Teóricos
Povidona/química
Propilenoglicol/química
Rafinose/química
Soluções/química
Trealose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Buffers); 0 (Cryoprotective Agents); 0 (Polymers); 0 (Solutions); 0 (Sugars); 059QF0KO0R (Water); 25702-74-3 (Ficoll); 6DC9Q167V3 (Propylene Glycol); B8WCK70T7I (Trehalose); FC72KVT52F (Ethylene Glycol); FZ989GH94E (Povidone); N5O3QU595M (Raffinose); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190713


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[PMID]:29023512
[Au] Autor:Bochimoto H; Matsuno N; Ishihara Y; Shonaka T; Koga D; Hira Y; Nishikawa Y; Furukawa H; Watanabe T
[Ad] Endereço:Health Care Administration Center, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
[Ti] Título:The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation.
[So] Source:PLoS One;12(10):e0186352, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects of warm machine perfusion preservation of liver grafts donated after cardiac death on the intracellular three-dimensional ultrastructure of the organelles in hepatocytes remain unclear. Here we analyzed comparatively the ultrastructure of the endomembrane systems in porcine hepatocytes under warm ischemia and successive hypothermic and midthermic machine perfusion preservation, a type of the warm machine perfusion. Porcine liver grafts which had a warm ischemia time of 60 minutes were perfused for 4 hours with modified University of Wisconsin gluconate solution. Group A grafts were preserved with hypothermic machine perfusion preservation at 8°C constantly for 4 hours. Group B grafts were preserved with rewarming up to 22°C by warm machine perfusion preservation for 4 hours. An analysis of hepatocytes after 60 minutes of warm ischemia by scanning electron microscope revealed the appearance of abnormal vacuoles and invagination of mitochondria. In the hepatocytes preserved by subsequent hypothermic machine perfusion preservation, strongly swollen mitochondria were observed. In contrast, the warm machine perfusion preservation could preserve the functional appearance of mitochondria in hepatocytes. Furthermore, abundant vacuoles and membranous structures sequestrating cellular organelles like autophagic vacuoles were frequently observed in hepatocytes after warm machine perfusion preservation. In conclusion, the ultrastructure of the endomembrane systems in the hepatocytes of liver grafts changed in accordance with the temperature conditions of machine perfusion preservation. In addition, temperature condition of the machine perfusion preservation may also affect the condition of the hepatic graft attributed to autophagy systems, and consequently alleviate the damage of the hepatocytes.
[Mh] Termos MeSH primário: Hepatócitos/ultraestrutura
Fígado/ultraestrutura
Preservação de Órgãos/normas
[Mh] Termos MeSH secundário: Adenosina/farmacologia
Alopurinol/farmacologia
Animais
Membrana Celular/ultraestrutura
Citocromos c/metabolismo
Morte
Feminino
Glutationa/farmacologia
Insulina/farmacologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Microscopia de Fluorescência
Proteínas Associadas aos Microtúbulos/metabolismo
Mitocôndrias/ultraestrutura
Soluções para Preservação de Órgãos/farmacologia
Rafinose/farmacologia
Suínos
Isquemia Quente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Microtubule-Associated Proteins); 0 (Organ Preservation Solutions); 0 (University of Wisconsin-lactobionate solution); 63CZ7GJN5I (Allopurinol); 9007-43-6 (Cytochromes c); GAN16C9B8O (Glutathione); K72T3FS567 (Adenosine); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186352


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[PMID]:28700604
[Au] Autor:Pereira Lima JJ; Buitink J; Lalanne D; Rossi RF; Pelletier S; da Silva EAA; Leprince O
[Ad] Endereço:Faculdade de Ciências Agronômicas, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo State, Brazil.
[Ti] Título:Molecular characterization of the acquisition of longevity during seed maturation in soybean.
[So] Source:PLoS One;12(7):e0180282, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seed longevity, defined as the ability to remain alive during storage, is an important agronomic factor. Poor longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in longevity. Longevity appeared at physiological maturity at the end of seed filling before maturation drying and progressively doubled until the seeds reached the dry state. The increase in longevity was associated with the expression of genes encoding protective chaperones such as heat shock proteins and the repression of nuclear and chloroplast genes involved in a range of chloroplast activities, including photosynthesis. An increase in the raffinose family oligosaccharides (RFO)/sucrose ratio together with changes in RFO metabolism genes was also associated with longevity. A gene co-expression network analysis revealed 27 transcription factors whose expression profiles were highly correlated with longevity. Eight of them were previously identified in the longevity network of Medicago truncatula, including homologues of ERF110, HSF6AB, NFXL1 and members of the DREB2 family. The network also contained several transcription factors associated with auxin and developmental cell fate during flowering, organ growth and differentiation. A transcriptional transition occurred concomitant with seed chlorophyll loss and detachment from the mother plant, suggesting the activation of a post-abscission program. This transition was enriched with AP2/EREBP and WRKY transcription factors and genes associated with growth, germination and post-transcriptional processes, suggesting that this program prepares the seed for the dry quiescent state and germination.
[Mh] Termos MeSH primário: Germinação/genética
Sementes/genética
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Ácidos Indolacéticos/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Rafinose/metabolismo
Sementes/crescimento & desenvolvimento
Sementes/metabolismo
Feijão de Soja/crescimento & desenvolvimento
Feijão de Soja/metabolismo
Sacarose/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Plant Proteins); 0 (Transcription Factors); 57-50-1 (Sucrose); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180282


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[PMID]:28518062
[Au] Autor:Hobeika MJ; Dar WA; Hall DR; Bynon JS
[Ad] Endereço:1 The Center for Abdominal Organ Transplantation and Regenerative Medicine, Department of Surgery, McGovern Medical School, The University of Texas Health Science Center at Houston and Memorial Hermann Hospital-Texas Medical Center, Houston, TX.
[Ti] Título:Retrograde Flushing of Living Donor Renal Allografts via the Renal Vein: A Simple, Effective Technique.
[So] Source:Transplantation;101(9):2111-2114, 2017 Sep.
[Is] ISSN:1534-6080
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Prograde flushing (PF) of living donor renal allografts with preservation solution via the renal artery or arteries is standard practice. PF may be difficult and potentially injurious to the donor kidney, especially in grafts with small or multiple arteries. In this report, we present our experience with retrograde flushing (RF) of 7 living donor kidneys via the renal vein. METHODS: Retrospective review of 7 consecutive living donor renal transplants performed using the RF technique was performed. The 7 preceding living donor renal transplants performed using the standard arterial PF technique served as a control group. RESULTS: All 7 recipients of RF kidneys experienced immediate graft function. At postoperative days 3 and 30, there was no difference in estimated glomerular filtration rate between the RF study group and PF controls. CONCLUSIONS: The RF technique is simple and safe, with results equivalent to the PF technique. The RF technique may be especially useful after recovering kidneys with small and/or multiple arteries.
[Mh] Termos MeSH primário: Transplante de Rim/métodos
Doadores Vivos
Nefrectomia
Soluções para Preservação de Órgãos/administração & dosagem
Veias Renais/cirurgia
Irrigação Terapêutica/métodos
[Mh] Termos MeSH secundário: Adenosina/administração & dosagem
Adenosina/efeitos adversos
Adulto
Idoso
Alopurinol/administração & dosagem
Alopurinol/efeitos adversos
Feminino
Taxa de Filtração Glomerular
Glutationa/administração & dosagem
Glutationa/efeitos adversos
Seres Humanos
Infusões Intravenosas
Insulina/administração & dosagem
Insulina/efeitos adversos
Transplante de Rim/efeitos adversos
Masculino
Meia-Idade
Soluções para Preservação de Órgãos/efeitos adversos
Rafinose/administração & dosagem
Rafinose/efeitos adversos
Recuperação de Função Fisiológica
Estudos Retrospectivos
Irrigação Terapêutica/efeitos adversos
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Organ Preservation Solutions); 0 (University of Wisconsin-lactobionate solution); 63CZ7GJN5I (Allopurinol); GAN16C9B8O (Glutathione); K72T3FS567 (Adenosine); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1097/TP.0000000000001525


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[PMID]:28335485
[Au] Autor:Pacifici S; Song J; Zhang C; Wang Q; Glahn RP; Kolba N; Tako E
[Ad] Endereço:Department of Animal Sciences, Cornell University, Ithaca, NY 14853, USA. sjp233@cornell.edu.
[Ti] Título:Intra Amniotic Administration of Raffinose and Stachyose Affects the Intestinal Brush Border Functionality and Alters Gut Microflora Populations.
[So] Source:Nutrients;9(3), 2017 Mar 19.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This study investigates the effectiveness of two types of prebiotics-stachyose and raffinose-which are present in staple food crops that are widely consumed in regions where dietary Fe deficiency is a health concern. The hypothesis is that these prebiotics will improve Fe status, intestinal functionality, and increase health-promoting bacterial populations in vivo ( ). By using the intra-amniotic administration procedure, prebiotic treatment solutions were injected in ovo (day 17 of embryonic incubation) with varying concentrations of a 1.0 mL pure raffinose or stachyose in 18 MΩ H2O. Four treatment groups (50, 100 mg·mL raffinose or stachyose) and two controls (18 MΩ H2O and non-injected) were utilized. At hatch the cecum, small intestine, liver, and blood were collected for assessment of the relative abundance of the gut microflora, relative expression of Fe-related genes and brush border membrane functional genes, hepatic ferritin levels, and hemoglobin levels, respectively. The prebiotic treatments increased the relative expression of brush border membrane functionality proteins ( < 0.05), decreased the relative expression of Fe-related proteins ( < 0.05), and increased villus surface area. Raffinose and stachyose increased the relative abundance of probiotics ( < 0.05) and decreased that of pathogenic bacteria. Raffinose and stachyose beneficially affected the gut microflora, Fe bioavailability, and brush border membrane functionality. Our investigations have led to a greater understanding of these prebiotics' effects on intestinal health and mineral metabolism.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal/efeitos dos fármacos
Intestinos/efeitos dos fármacos
Microvilosidades/efeitos dos fármacos
Oligossacarídeos/administração & dosagem
Rafinose/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Bifidobacterium/isolamento & purificação
Disponibilidade Biológica
Galinhas
Clostridium/isolamento & purificação
Modelos Animais de Doenças
Escherichia coli/isolamento & purificação
Ferritinas/metabolismo
Intestinos/metabolismo
Intestinos/microbiologia
Ferro/sangue
Lactobacillus/isolamento & purificação
Fígado/metabolismo
Microvilosidades/metabolismo
Microvilosidades/microbiologia
Prebióticos/administração & dosagem
Probióticos/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Prebiotics); 25VX64653N (stachyose); 9007-73-2 (Ferritins); E1UOL152H7 (Iron); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


  7 / 2498 MEDLINE  
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[PMID]:28213171
[Au] Autor:Acket S; Degournay A; Merlier F; Thomasset B
[Ad] Endereço:Sorbonne Universités, Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Technologie de Compiègne, 60203 Compiègne Cedex, France. Electronic address: sebastien.acket@utc.fr.
[Ti] Título:13C labeling analysis of sugars by high resolution-mass spectrometry for metabolic flux analysis.
[So] Source:Anal Biochem;527:45-48, 2017 Jun 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography-high resolution mass spectrometry, without derivatized procedure, adapted for Metabolic flux analysis. Our results showed a good sensitivity, reproducibility and better accuracy to determine isotopic enrichments of free sugars compared to our previous methods [5, 6].
[Mh] Termos MeSH primário: Linho/metabolismo
Marcação por Isótopo/métodos
Análise do Fluxo Metabólico/métodos
Sementes/metabolismo
[Mh] Termos MeSH secundário: Isótopos de Carbono
Cromatografia Líquida
Linho/química
Linho/crescimento & desenvolvimento
Frutose/biossíntese
Frutose/isolamento & purificação
Glucose/biossíntese
Glucose/isolamento & purificação
Maltose/biossíntese
Maltose/isolamento & purificação
Espectrometria de Massas
Rafinose/biossíntese
Rafinose/isolamento & purificação
Reprodutibilidade dos Testes
Sementes/química
Sementes/crescimento & desenvolvimento
Sensibilidade e Especificidade
Sacarose/isolamento & purificação
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 30237-26-4 (Fructose); 57-50-1 (Sucrose); 69-79-4 (Maltose); IY9XDZ35W2 (Glucose); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


  8 / 2498 MEDLINE  
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[PMID]:28199749
[Au] Autor:Pan M; Kumaree KK; Shah NP
[Ad] Endereço:Food and Nutritional Science, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
[Ti] Título:Physiological Changes of Surface Membrane in Lactobacillus with Prebiotics.
[So] Source:J Food Sci;82(3):744-750, 2017 Mar.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synbiotics are always considered to be beneficial in healthy manipulation of gut environment; however, the purpose of this research was to investigate the dominance of synbiotic over the individual potential of probiotics and prebiotics. Four different types of prebiotics, fructo-oligosaccharides, raffinose, inulin, and cellobiose, were evaluated based on their varying degree of polymerization, combined each with 2 different Lactobacilli strains, including Lactobacillus paracasei 276 and Lactobacillus plantarum WCFS1. The effects of synbiotics combination on the surface structure were evaluated by analyzing auto-aggregation, membrane hydrophobicity, and adhesion to Caco-2 cells. Our results showed that both Lactobacilli exhibited significantly greater degree of attachment to Caco-2 cells (23.31% and 16.85%, respectively) when using cellobiose as a substrate than with other prebiotics (P < 0.05). Intestinal adhesion ability was in correlation with the percent of auto-aggregation, both Lactobacillus exhibited higher percent of auto-aggregation in cellobiose compared to other prebiotics. These behavioral changes in terms of attachment and auto-aggregation were further supported with the changes noticed from infrared spectra (FT-IR).
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Lactobacillus/efeitos dos fármacos
Oligossacarídeos/farmacologia
Prebióticos
Probióticos
Simbióticos
[Mh] Termos MeSH secundário: Células CACO-2
Membrana Celular/fisiologia
Celobiose/farmacologia
Frutose/farmacologia
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Intestinos/microbiologia
Inulina/farmacologia
Lactobacillus/crescimento & desenvolvimento
Rafinose/farmacologia
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligosaccharides); 0 (Prebiotics); 16462-44-5 (Cellobiose); 30237-26-4 (Fructose); 9005-80-5 (Inulin); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13608


  9 / 2498 MEDLINE  
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[PMID]:28194034
[Au] Autor:Abe T; Yazawa K; Fujino M; Imamura R; Hatayama N; Kakuta Y; Tsutahara K; Okumi M; Ichimaru N; Kaimori JY; Isaka Y; Seki K; Takahara S; Li XK; Nonomura N
[Ad] Endereço:Department of Specific Organ Regulation (Urology), Osaka University Graduate School of Medicine, Osaka, Japan.
[Ti] Título:High-pressure carbon monoxide preserves rat kidney grafts from apoptosis and inflammation.
[So] Source:Lab Invest;97(4):468-477, 2017 Apr.
[Is] ISSN:1530-0307
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Renal ischemia-reperfusion (I/R) injury is unavoidable in kidney transplantation (KTx) and frequently influences both short- and long-term allograft survival. Carbon monoxide (CO) has attracted attention as a medical gas with anti-inflammatory and anti-apoptotic effects. We investigated a new strategy for organ preservation using ex vivo application of high-pressure CO in an experimental rat KTx model. We preserved kidney grafts using a high-pressure chamber filled with mixed gases composed of CO and O . We found that cold I/R injury resulted in progressive deterioration of renal graft function in University of Wisconsin solution, whereas CO significantly improved renal function. We confirmed that CO decreased oxidative stress and mRNA expression of proinflammatory cytokines and inhibited tubular apoptosis in the early phases. Western blot analysis demonstrated that CO increased phosphatidylinositol-3 kinase and phosphorylation of Akt and p38 mitogen-activated protein kinase. Furthermore, CO significantly alleviated tubular injury scores and suppressed the development of interstitial fibrosis at 100 days after KTx. Thus, high-pressure mixed CO and O gases successfully preserved rat kidney grafts for 24 h by protecting tubular epithelial cells from apoptosis and inhibiting inflammation.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Monóxido de Carbono/farmacologia
Inflamação/prevenção & controle
Transplante de Rim/métodos
Rim/efeitos dos fármacos
Preservação de Órgãos/métodos
[Mh] Termos MeSH secundário: Adenosina/farmacologia
Alopurinol/farmacologia
Animais
Western Blotting
Temperatura Baixa
Citocinas/genética
Citocinas/metabolismo
Expressão Gênica/efeitos dos fármacos
Glutationa/farmacologia
Sobrevivência de Enxerto/efeitos dos fármacos
Inflamação/genética
Inflamação/metabolismo
Mediadores da Inflamação/metabolismo
Insulina/farmacologia
Rim/metabolismo
Túbulos Renais/efeitos dos fármacos
Túbulos Renais/metabolismo
Túbulos Renais/patologia
Masculino
Soluções para Preservação de Órgãos/farmacologia
Oxigênio/farmacologia
Pressão Parcial
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/metabolismo
Rafinose/farmacologia
Ratos Endogâmicos Lew
Traumatismo por Reperfusão
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators); 0 (Insulin); 0 (Organ Preservation Solutions); 0 (University of Wisconsin-lactobionate solution); 63CZ7GJN5I (Allopurinol); 7U1EE4V452 (Carbon Monoxide); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); GAN16C9B8O (Glutathione); K72T3FS567 (Adenosine); N5O3QU595M (Raffinose); S88TT14065 (Oxygen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1038/labinvest.2016.157


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[PMID]:28068432
[Au] Autor:Ivamoto ST; Reis O; Domingues DS; Dos Santos TB; de Oliveira FF; Pot D; Leroy T; Vieira LG; Carazzolle MF; Pereira GA; Pereira LF
[Ad] Endereço:Programa de Pós-Graduação em Genética e Biologia Molecular, Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Brazil.
[Ti] Título:Transcriptome Analysis of Leaves, Flowers and Fruits Perisperm of Coffea arabica L. Reveals the Differential Expression of Genes Involved in Raffinose Biosynthesis.
[So] Source:PLoS One;12(1):e0169595, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coffea arabica L. is an important crop in several developing countries. Despite its economic importance, minimal transcriptome data are available for fruit tissues, especially during fruit development where several compounds related to coffee quality are produced. To understand the molecular aspects related to coffee fruit and grain development, we report a large-scale transcriptome analysis of leaf, flower and perisperm fruit tissue development. Illumina sequencing yielded 41,881,572 high-quality filtered reads. De novo assembly generated 65,364 unigenes with an average length of 1,264 bp. A total of 24,548 unigenes were annotated as protein coding genes, including 12,560 full-length sequences. In the annotation process, we identified nine candidate genes related to the biosynthesis of raffinose family oligossacarides (RFOs). These sugars confer osmoprotection and are accumulated during initial fruit development. Four genes from this pathway had their transcriptional pattern validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we identified ~24,000 putative target sites for microRNAs (miRNAs) and 134 putative transcriptionally active transposable elements (TE) sequences in our dataset. This C. arabica transcriptomic atlas provides an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Our results are the starting point for enhancing our knowledge about the coffee genes that are transcribed during the flowering and initial fruit development stages.
[Mh] Termos MeSH primário: Coffea/genética
Coffea/metabolismo
Flores/genética
Frutas/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Folhas de Planta/genética
Rafinose/biossíntese
[Mh] Termos MeSH secundário: Biologia Computacional/métodos
Elementos de DNA Transponíveis
Anotação de Sequência Molecular
Fases de Leitura Aberta
Especificidade de Órgãos/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); N5O3QU595M (Raffinose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169595



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