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[PMID]:28459063
[Au] Autor:Wang C; Zhao J; Qiu C; Wang S; Shen Y; Du B; Ding Y; Bao X
[Ad] Endereço:College of Life Sciences and Shandong Key Laboratory of Agricultural Microbiology and National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an, China.
[Ti] Título:Coutilization of D-Glucose, D-Xylose, and L-Arabinose in by Coexpressing the Metabolic Pathways and Evolutionary Engineering.
[So] Source:Biomed Res Int;2017:5318232, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Efficient and cost-effective fuel ethanol production from lignocellulosic materials requires simultaneous cofermentation of all hydrolyzed sugars, mainly including D-glucose, D-xylose, and L-arabinose. is a traditional D-glucose fermenting strain and could utilize D-xylose and L-arabinose after introducing the initial metabolic pathways. The efficiency and simultaneous coutilization of the two pentoses and D-glucose for ethanol production in still need to be optimized. Previously, we constructed an L-arabinose-utilizing BSW3AP. In this study, we further introduced the XI and XR-XDH metabolic pathways of D-xylose into BSW3AP to obtain D-glucose, D-xylose, and L-arabinose cofermenting strain. Benefits of evolutionary engineering: the resulting strain BSW4XA3 displayed a simultaneous coutilization of D-xylose and L-arabinose with similar consumption rates, and the D-glucose metabolic capacity was not decreased. After 120 h of fermentation on mixed D-glucose, D-xylose, and L-arabinose, BSW4XA3 consumed 24% more amounts of pentoses and the ethanol yield of mixed sugars was increased by 30% than that of BSW3AP. The resulting strain BSW4XA3 was a useful chassis for further enhancing the coutilization efficiency of mixed sugars for bioethanol production.
[Mh] Termos MeSH primário: Engenharia Metabólica/métodos
Redes e Vias Metabólicas/genética
Monossacarídeos/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Biocombustíveis
Etanol
Fermentação
Monossacarídeos/análise
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Monosaccharides); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1155/2017/5318232


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[PMID]:28460247
[Au] Autor:Zweckmair T; Schiehser S; Rosenau T; Potthast A
[Ad] Endereço:Division of Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Konrad-Lorenz-Strasse 24, A-3430 Tulln, Austria.
[Ti] Título:Improved quantification of monosaccharides in complex lignocellulosic biomass matrices: A gas chromatography-mass spectrometry based approach.
[So] Source:Carbohydr Res;446-447:7-12, 2017 Jun 29.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel method for the precise and accurate quantification of wood monosaccharides by gas-chromatography mass-spectrometry in complex lignocellulosic biomass matrices is presented. Instead of using the syn- or the anti-peak obtained by blocking the anomeric center using e.g. oximation, the sum of each syn- and anti-peak pair is used for quantification rendering the approach of the apparently constant syn- and anti-peak area ratio. Each wood monosaccharide syn- and anti-peak could essentially be distinguished upon O-ethoximation followed by trifluoroacetylation and separation on a 14% cyanopropyl/phenyl- 86% dimethylpoly-siloxane column. Additionally, internal standardization was carried out applying isotopically labeled compounds. Hence, the analytical figures of merit, such as precision and accuracy, could be substantially improved compared to standard gas-chromatography mass spectrometry as well as high-performance anion exchange chromatography (HPAEC) coupled to pulsed-amperometric detection (PAD). The applicability of the novel gas-chromatography mass-spectrometry approach is demonstrated by analysis of five selected lignocellulosic biomass sample matrices typically occurring in a biorefinery scenario. Even in exceedingly complex sample matrices precise and accurate data are obtained according to the methodology presented.
[Mh] Termos MeSH primário: Biomassa
Cromatografia Gasosa-Espectrometria de Massas
Lignina/química
Monossacarídeos/análise
[Mh] Termos MeSH secundário: Madeira/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monosaccharides); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29223588
[Au] Autor:Baek J; Lee D; Lee TK; Song JH; Lee JS; Lee S; Yoo SW; Kang KS; Moon E; Lee S; Kim KH
[Ad] Endereço:School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
[Ti] Título:(-)-9'-O-(α-l-Rhamnopyranosyl)lyoniresinol from Lespedeza cuneata suppresses ovarian cancer cell proliferation through induction of apoptosis.
[So] Source:Bioorg Med Chem Lett;28(2):122-128, 2018 01 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lespedeza cuneata (Dum. Cours.) G. Don. (Fabaceae), known as Chinese bushclover or sericea lespedeza, has been used in traditional medicine to treat diabetes, hematuria, and insomnia, and it has been reported that bioactive compounds from L. cuneata possess various pharmacological properties. However, there has been no study to determine the active compounds from L. cuneata with potential activity against ovarian cancer. This study aimed to isolate cytotoxic compounds from L. cuneata and identify the molecular mechanisms underlying the apoptosis pathway in ovarian cancer cells. Based on cytotoxic activity identified in the screening test, chemical investigation of the active fraction of L. cuneata led to the isolation of nine compounds including four lignanosides (1-4), three flavonoid glycosides (5-7), and two phenolics (8-9). Cytotoxicity and the molecular mechanism were examined by methyl thiazolyl tetrazolium (MTT) assay and Western blot analysis. Of the isolated compounds, (-)-9'-O-(α-l-rhamnopyranosyl)lyoniresinol (3) demonstrated the strongest effect in suppressing A2780 human ovarian carcinoma cell proliferation in a dose-dependent manner, with an IC value of 35.40 ±â€¯2.78 µM. Control A2780 cells had normal morphology, whereas cell blebbing, shrinkage, and condensation were observed after treatment with compound 3. Western blotting analysis showed that compound 3 inhibited A2780 human ovarian cancer cell viability by activating caspase-8, caspase-3, and PARP, which contributed to apoptotic cell death. These results suggest that (-)-9'-O-(α-l-rhamnopyranosyl)lyoniresinol (3) has potent anticancer activities against A2780 human ovarian carcinoma cells through the extrinsic apoptotic pathway. Therefore, (-)-9'-O-(α-l-rhamnopyranosyl)lyoniresinol is an excellent candidate for the development of novel chemotherapeutics.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Lespedeza/química
Monossacarídeos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Estrutura Molecular
Monossacarídeos/química
Monossacarídeos/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Monosaccharides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE


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[PMID]:29311516
[Au] Autor:Katsuyama A; Sato K; Yakushiji F; Matsumaru T; Ichikawa S
[Ad] Endereço:Faculty of Pharmaceutical Science, Hokkaido University.
[Ti] Título:Solid-Phase Modular Synthesis of Park Nucleotide and Lipids I and II Analogues.
[So] Source:Chem Pharm Bull (Tokyo);66(1):84-95, 2018.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A solid-phase synthesis of Park nucleotide as well as lipids I and II analogues, which is applicable to the synthesis of a range of analogues, is described in this work. This technique allows highly functionalized macromolecules to be modularly labeled. Multiple steps are used in a short time (4 d) with a single purification step to synthesize the molecules by solid-phase synthesis.
[Mh] Termos MeSH primário: Monossacarídeos/síntese química
Nucleotídeos/síntese química
Oligopeptídeos/síntese química
Técnicas de Síntese em Fase Sólida
Uridina Difosfato Ácido N-Acetilmurâmico/análogos & derivados
[Mh] Termos MeSH secundário: Conformação Molecular
Monossacarídeos/química
Nucleotídeos/química
Oligopeptídeos/química
Uridina Difosfato Ácido N-Acetilmurâmico/síntese química
Uridina Difosfato Ácido N-Acetilmurâmico/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monosaccharides); 0 (Nucleotides); 0 (Oligopeptides); 0 (Uridine Diphosphate N-Acetylmuramic Acid); 0 (lipid I); 0 (muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00828


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[PMID]:29364604
[Au] Autor:Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV
[Ti] Título:[Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.]
[So] Source:Mikrobiologiia;85(5):568-579, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems.
[Mh] Termos MeSH primário: Deinococcus/metabolismo
Processos Heterotróficos/fisiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/efeitos da radiação
Aminoácidos/metabolismo
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Bacteroidetes/efeitos da radiação
Betaproteobacteria/classificação
Betaproteobacteria/genética
Betaproteobacteria/isolamento & purificação
Betaproteobacteria/efeitos da radiação
Biodiversidade
Deinococcus/classificação
Deinococcus/isolamento & purificação
Deinococcus/efeitos da radiação
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/efeitos da radiação
Dissacarídeos/metabolismo
Ecossistema
Firmicutes/classificação
Firmicutes/genética
Firmicutes/isolamento & purificação
Firmicutes/efeitos da radiação
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/efeitos da radiação
Processos Heterotróficos/efeitos da radiação
Consórcios Microbianos/efeitos da radiação
Monossacarídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Proteobactérias/efeitos da radiação
Sibéria
Propriedades de Superfície
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29210296
[Au] Autor:Zhu LJ; Yi S; Li X; Chen HF; Ming M; Zhang X; Yao XS
[Ad] Endereço:a Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
[Ti] Título:C-glycosides from the stems of Calophyllum membranaceum.
[So] Source:J Asian Nat Prod Res;20(1):49-54, 2018 Jan.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three new C-glycosides, calophymembransides D-F (1-3), were isolated from the stems of Calophyllum membranaceum Gardn. et Champ.. The structures were assigned on the basis of spectroscopic data. RXRα transcriptional inhibition and α-glucosidase inhibition assays indicated that all the isolates were inactive.
[Mh] Termos MeSH primário: Calophyllum/química
Inibidores de Glicosídeo Hidrolases/farmacologia
Monossacarídeos/isolamento & purificação
[Mh] Termos MeSH secundário: Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Estrutura Molecular
Monossacarídeos/química
Caules de Planta/química
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (C-glycoside); 0 (Glycoside Hydrolase Inhibitors); 0 (Monosaccharides); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2017.1409734


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[PMID]:29216560
[Au] Autor:Yuan MC; Yeh TK; Chen CT; Song JS; Huang YC; Hsieh TC; Huang CY; Huang YL; Wang MH; Wu SH; Yao CH; Chao YS; Lee JC
[Ad] Endereço:Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli 35053, Taiwan.
[Ti] Título:Identification of an oxime-containing C-glucosylarene as a potential inhibitor of sodium-dependent glucose co-transporter 2.
[So] Source:Eur J Med Chem;143:611-620, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Treatment of hyperglycemia with drugs that block renal glucose reabsorption via inhibition of sodium-dependent glucose cotransporter 2 (SGLT2) is a novel approach to diabetes management. In this study, twenty-seven aryl C-glycosides bearing a C=N/C-N linkage at the glucosyl C6 position were designed, synthesized and evaluated for their inhibitory activity against human SGLT2 (hSGLT2). Compounds with good hSGLT2 inhibition were further investigated to determine their selectivity over hSGLT1. Of these, five representative aryl C-glycosides were chosen for pharmacokinetic analysis. Oxime 2a was determined to have the most promising pharmacokinetic properties and was selected for in vivo glucosuria and plasma glucose level studies, which found it to exhibit comparable efficacy to dapagliflozin (1). Furthermore, 2a was not found to exhibit either significant cytotoxicity (CC > 50 µM) or human ether-a-go-go related gene (hERG) inhibition (2% inhibition at 10 µM). Taken together, these efforts culminated in the discovery of oxime 2a as a potential SGLT2 inhibitor.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/tratamento farmacológico
Hipoglicemiantes/farmacologia
Monossacarídeos/farmacologia
Oximas/farmacologia
Transportador 2 de Glucose-Sódio/antagonistas & inibidores
[Mh] Termos MeSH secundário: Administração Intravenosa
Administração Oral
Animais
Compostos Benzidrílicos/administração & dosagem
Compostos Benzidrílicos/farmacologia
Glicemia/efeitos dos fármacos
Relação Dose-Resposta a Droga
Glucose/análise
Glucosídeos/administração & dosagem
Glucosídeos/farmacologia
Seres Humanos
Hipoglicemiantes/administração & dosagem
Hipoglicemiantes/química
Estrutura Molecular
Monossacarídeos/química
Oximas/administração & dosagem
Oximas/química
Ratos
Ratos Sprague-Dawley
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(3-(4-ethoxybenzyl)-4-chlorophenyl)-6-hydroxymethyltetrahydro-2H-pyran-3,4,5-triol); 0 (Benzhydryl Compounds); 0 (Blood Glucose); 0 (C-glucosylarene); 0 (Glucosides); 0 (Hypoglycemic Agents); 0 (Monosaccharides); 0 (Oximes); 0 (SLC5A2 protein, human); 0 (Sodium-Glucose Transporter 2); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


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[PMID]:29205038
[Au] Autor:Bonte A; Schweiger R; Pons C; Wagner C; Brühl L; Matthäus B; Müller C
[Ad] Endereço:Department of Safety and Quality of Cereals, Max Rubner-Institut, Federal Research Institute of Nutrition and Food , Schützenberg 12, 32756 Detmold, Germany.
[Ti] Título:Metabolic Changes during Storage of Brassica napus Seeds under Moist Conditions and the Consequences for the Sensory Quality of the Resulting Virgin Oil.
[So] Source:J Agric Food Chem;65(50):11073-11084, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Virgin rapeseed (Brassica napus) oil is a valuable niche product, if delivered with a high quality. In this study, the effects of moist storage of B. napus seeds for 1 to 4 days on the seed metabolome and the chemo-sensory properties of the produced oils were determined. The concentrations of several primary metabolites, including monosaccharides and amino acids, rapidly increased in the seeds, probably indicating the breakdown of storage compounds to support seed germination. Seed concentrations of indole glucosinolates increased with a slight time offset suggesting that amino acids may be used to modify secondary metabolism. The volatile profiles of the oils were pronouncedly influenced by moist seed storage, with the sensory quality of the oils decreasing. This study provides a direct time-resolved link between seed metabolism under moist conditions and the quality of the resulting oils, thereby emphasizing the crucial role of dry seed storage in ensuring high oil quality.
[Mh] Termos MeSH primário: Brassica napus/química
Óleos Vegetais/química
Sementes/metabolismo
Água/análise
[Mh] Termos MeSH secundário: Aminoácidos/análise
Aminoácidos/metabolismo
Brassica napus/crescimento & desenvolvimento
Brassica napus/metabolismo
Armazenamento de Alimentos
Seres Humanos
Monossacarídeos/análise
Monossacarídeos/metabolismo
Óleos Vegetais/metabolismo
Sementes/química
Sementes/crescimento & desenvolvimento
Paladar
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Monosaccharides); 0 (Plant Oils); 059QF0KO0R (Water)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04149


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[PMID]:29174810
[Au] Autor:Liu Y; Peng Y; Lu J; Wang J; Ma H; Song C; Liu B; Qiao Y; Yu W; Wu J; Chang J
[Ad] Endereço:College of Chemistry and Molecular Engineering, Zhengzhou University, Henan 450001, PR China.
[Ti] Título:Design, synthesis, and biological evaluation of new 1,2,3-triazolo-2'-deoxy-2'-fluoro- 4'-azido nucleoside derivatives as potent anti-HBV agents.
[So] Source:Eur J Med Chem;143:137-149, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Novel drugs are urgently needed to combat hepatitis B virus (HBV) infection due to drug-resistant virus. In this paper, a series of novel 4-monosubstituted 2'-deoxy-2'-ß-fluoro-4'-azido-ß-d-arabinofuranosyl 1,2,3-triazole nucleoside analogues (1a-g) were designed, synthesized and screened for in vitro anti-HBV activity. At 5.0 µM in the cellular model, all the synthetic compounds display activities comparable to that of the positive control, lamivudine at 20 µM. Of the compounds tested, the amide-substituted analogue (1a) shows the most promising anti-HBV activity and low cytotoxicity in the cell model. In particular, it retains excellent activity against lamivudine-resistant HBV mutants. In duck HBV (DHBV)-infected duck models, both the serum and liver DHBV DNA levels (67.4% and 53.3%, respectively) were reduced markedly by the treatment with 1a. Analysis of the structure of HBV polymer/1a-triphosphate (1a-TP) complex shows that 1a-TP is stabilized by specific van der Waals interactions with the enzyme residues arising from 4-amino-1,2,3-triazole and the 4'-azido group.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Desenho de Drogas
Vírus da Hepatite B do Pato/efeitos dos fármacos
Vírus da Hepatite B/efeitos dos fármacos
Hepatite B/tratamento farmacológico
Monossacarídeos/farmacologia
Nucleosídeos/farmacologia
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antivirais/síntese química
Antivirais/química
Sobrevivência Celular/efeitos dos fármacos
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Patos
Células Hep G2
Vírus da Hepatite B/genética
Seres Humanos
Modelos Moleculares
Estrutura Molecular
Monossacarídeos/síntese química
Monossacarídeos/química
Nucleosídeos/síntese química
Nucleosídeos/química
Relação Estrutura-Atividade
Triazóis/síntese química
Triazóis/química
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2'-deoxy-2'-beta-fluoro-4'-azido-b-D-arabinofuranosyl 1,2,3-triazole); 0 (Antiviral Agents); 0 (Monosaccharides); 0 (Nucleosides); 0 (Triazoles)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:28543285
[Au] Autor:Zhang XK; Lan YB; Zhu BQ; Xiang XF; Duan CQ; Shi Y
[Ad] Endereço:Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China.
[Ti] Título:Changes in monosaccharides, organic acids and amino acids during Cabernet Sauvignon wine ageing based on a simultaneous analysis using gas chromatography-mass spectrometry.
[So] Source:J Sci Food Agric;98(1):104-112, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Monosaccharides, organic acids and amino acids are the important flavour-related components in wines. The aim of this article is to develop and validate a method that could simultaneously analyse these compounds in wine based on silylation derivatisation and gas chromatography-mass spectrometry (GC-MS), and apply this method to the investigation of the changes of these compounds and speculate upon their related influences on Cabernet Sauvignon wine flavour during wine ageing. This work presented a new approach for wine analysis and provided more information concerning red wine ageing. RESULTS: This method could simultaneously quantitatively analyse 2 monosaccharides, 8 organic acids and 13 amino acids in wine. A validation experiment showed good linearity, sensitivity, reproducibility and recovery. Multiple derivatives of five amino acids have been found but their effects on quantitative analysis were negligible, except for methionine. The evolution pattern of each category was different, and we speculated that the corresponding mechanisms involving microorganism activities, physical interactions and chemical reactions had a great correlation with red wine flavours during ageing. CONCLUSION: Simultaneously quantitative analysis of monosaccharides, organic acids and amino acids in wine was feasible and reliable and this method has extensive application prospects. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Ácidos/química
Aminoácidos/química
Cromatografia Gasosa-Espectrometria de Massas/métodos
Monossacarídeos/química
Vinho/análise
[Mh] Termos MeSH secundário: Seres Humanos
Odorantes/análise
Olfato
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids); 0 (Amino Acids); 0 (Monosaccharides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8444



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