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[PMID]: 29273433 [Au] Autor: Shi YJ; Wang RT; Chu YH; Chen YJ; Tang CC; Fu YS; Lee YC; Wang LJ; Huang CH; Chang LS [Ad] Endereço: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. [Ti] Título: Membrane-damaging activities of mannosylated ovalbumin are involved in its antibacterial action. [So] Source: Arch Biochem Biophys;639:1-8, 2018 02 01. [Is] ISSN: 1096-0384 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Mannosylated ovalbumin (Man-OVA) prepared by modification of carboxyl groups with p-aminophenyl α-d-mannopyranoside shows an increase of net positive charge, which may enhance its binding to bacterial membrane. Thus, we aimed to investigate whether Man-OVA exerts antibacterial activity on Escherichia coli and Staphylococcus aureus via membrane-perturbing effect. Man-OVA inhibited the growth of E. coli and S. aureus, whereas ovalbumin (OVA) did not show any antibacterial activity. Moreover, Man-OVA induced an increase in the membrane permeability of E. coli and S. aureus, which was positively correlated to its bactericidal action. Morphological examination using scanning electron microscopy revealed that Man-OVA disrupted the bacterial membrane integrity. Destabilization of the lipopolysaccharide (LPS) layer and inhibition of lipoteichoic acid (LTA) biosynthesis in the cell wall increased the bactericidal effect of Man-OVA. In contrast to OVA, Man-OVA also induced leakage of bacterial membrane-mimicking liposomes. Color transformation of phospholipid/polydiacetylene membrane assay revealed that the membrane-interaction mode of Man-OVA was distinct from that of OVA. LPS and LTA suppressed the membrane-damaging activity of Man-OVA, whereas an increase in the Man-OVA concentration attenuated the inhibitory action of LPS and LTA. Taken together, our data indicate that the bactericidal activity of Man-OVA depends strongly on its ability to induce membrane permeability. [Mh] Termos MeSH primário: Antibacterianos Membrana Celular Escherichia coli Manose Ovalbumina Staphylococcus aureus [Mh] Termos MeSH secundário: Antibacterianos/química Antibacterianos/farmacologia Membrana Celular/química Membrana Celular/metabolismo Parede Celular/química Parede Celular/metabolismo Escherichia coli/química Escherichia coli/metabolismo Lipopolissacarídeos/química Lipopolissacarídeos/metabolismo Manose/química Manose/farmacologia Microscopia Eletrônica de Varredura Ovalbumina/química Ovalbumina/farmacologia Staphylococcus aureus/química Staphylococcus aureus/metabolismo Ácidos Teicoicos/química Ácidos Teicoicos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Lipopolysaccharides); 0 (Teichoic Acids); 56411-57-5 (lipoteichoic acid); 9006-59-1 (Ovalbumin); PHA4727WTP (Mannose) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180214 [Lr] Data última revisão: 180214 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171224 [St] Status: MEDLINE

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[PMID]: 28458347 [Au] Autor: Panthavee W; Noda M; Danshiitsoodol N; Kumagai T; Sugiyama M [Ad] Endereço: Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University. [Ti] Título: Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand. [So] Source: Biol Pharm Bull;40(5):621-629, 2017. [Is] ISSN: 1347-5215 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plant-derived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources. [Mh] Termos MeSH primário: Frutas/microbiologia Lactobacillus/química Pediococcus/química Polissacarídeos/química Polissacarídeos/farmacologia [Mh] Termos MeSH secundário: Meios de Cultura DNA Bacteriano/genética Fermentação Galactose/química Glucose/química Hialuronoglucosaminidase/antagonistas & inibidores Ácido Láctico/metabolismo Lactobacillus/genética Manose/química Pediococcus/genética Açúcares/análise Temperatura Ambiente Tailândia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Culture Media); 0 (DNA, Bacterial); 0 (Polysaccharides); 0 (Sugars); 33X04XA5AT (Lactic Acid); EC 3.2.1.35 (Hyaluronoglucosaminidase); IY9XDZ35W2 (Glucose); PHA4727WTP (Mannose); X2RN3Q8DNE (Galactose) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE [do] DOI: 10.1248/bpb.b16-00856

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[PMID]: 28743912 [Au] Autor: Gandini R; Reichenbach T; Tan TC; Divne C [Ad] Endereço: School of Biotechnology, KTH Royal Institute of Technology, S-10691, Stockholm, Sweden. [Ti] Título: Structural basis for dolichylphosphate mannose biosynthesis. [So] Source: Nat Commun;8(1):120, 2017 07 25. [Is] ISSN: 2041-1723 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Protein glycosylation is a critical protein modification. In biogenic membranes of eukaryotes and archaea, these reactions require activated mannose in the form of the lipid conjugate dolichylphosphate mannose (Dol-P-Man). The membrane protein dolichylphosphate mannose synthase (DPMS) catalyzes the reaction whereby mannose is transferred from GDP-mannose to the dolichol carrier Dol-P, to yield Dol-P-Man. Failure to produce or utilize Dol-P-Man compromises organism viability, and in humans, several mutations in the human dpm1 gene lead to congenital disorders of glycosylation (CDG). Here, we report three high-resolution crystal structures of archaeal DPMS from Pyrococcus furiosus, in complex with nucleotide, donor, and glycolipid product. The structures offer snapshots along the catalytic cycle, and reveal how lipid binding couples to movements of interface helices, metal binding, and acceptor loop dynamics to control critical events leading to Dol-P-Man synthesis. The structures also rationalize the loss of dolichylphosphate mannose synthase function in dpm1-associated CDG.The generation of glycolipid dolichylphosphate mannose (Dol-P-Man) is a critical step for protein glycosylation and GPI anchor synthesis. Here the authors report the structure of dolichylphosphate mannose synthase in complex with bound nucleotide and donor to provide insight into the mechanism of Dol-P-Man synthesis. [Mh] Termos MeSH primário: Proteínas Arqueais/metabolismo Manose/biossíntese Manosiltransferases/metabolismo Pyrococcus furiosus/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Proteínas Arqueais/química Proteínas Arqueais/genética Sítios de Ligação/genética Biocatálise Cristalografia por Raios X Manose/química Manosiltransferases/química Manosiltransferases/genética Modelos Moleculares Domínios Proteicos Pyrococcus furiosus/enzimologia Pyrococcus furiosus/genética Proteínas Recombinantes/química Proteínas Recombinantes/metabolismo Homologia de Sequência de Aminoácidos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Archaeal Proteins); 0 (Recombinant Proteins); EC 2.4.1.- (Mannosyltransferases); EC 2.4.1.109 (protein O-mannosyltransferase); PHA4727WTP (Mannose) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE [do] DOI: 10.1038/s41467-017-00187-2

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[PMID]: 28994411 [Au] Autor: Gristick HB; Wang H; Bjorkman PJ [Ad] Endereço: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. [Ti] Título: X-ray and EM structures of a natively glycosylated HIV-1 envelope trimer. [So] Source: Acta Crystallogr D Struct Biol;73(Pt 10):822-828, 2017 Oct 01. [Is] ISSN: 2059-7983 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The structural and biochemical characterization of broadly neutralizing anti-HIV-1 antibodies (bNAbs) has been essential in guiding the design of potential vaccines to prevent infection by HIV-1. While these studies have revealed critical mechanisms by which bNAbs recognize and/or accommodate N-glycans on the trimeric envelope glycoprotein (Env), they have been limited to the visualization of high-mannose glycan forms only, since heterogeneity introduced from the presence of complex glycans makes it difficult to obtain high-resolution structures. 3.5 and 3.9 Šresolution crystal structures of the HIV-1 Env trimer with fully processed and native glycosylation were solved, revealing a glycan shield of high-mannose and complex-type N-glycans that were used to define the complete epitopes of two bNAbs. Here, the refinement of the N-glycans in the crystal structures is discussed and comparisons are made with glycan densities in glycosylated Env structures derived by single-particle cryo-electron microscopy. [Mh] Termos MeSH primário: HIV-1/química Manose/análise Polissacarídeos/análise Produtos do Gene env do Vírus da Imunodeficiência Humana/química [Mh] Termos MeSH secundário: Anticorpos Neutralizantes/química Microscopia Crioeletrônica Cristalografia por Raios X Glicosilação Anticorpos Anti-HIV/química Proteína gp120 do Envelope de HIV/química Proteína gp120 do Envelope de HIV/ultraestrutura Proteína gp41 do Envelope de HIV/química Proteína gp41 do Envelope de HIV/ultraestrutura Infecções por HIV/virologia HIV-1/ultraestrutura Seres Humanos Modelos Moleculares Conformação Proteica Multimerização Proteica Produtos do Gene env do Vírus da Imunodeficiência Humana/ultraestrutura [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Neutralizing); 0 (HIV Antibodies); 0 (HIV Envelope Protein gp120); 0 (HIV Envelope Protein gp41); 0 (Polysaccharides); 0 (env Gene Products, Human Immunodeficiency Virus); PHA4727WTP (Mannose) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171101 [Lr] Data última revisão: 171101 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171011 [St] Status: MEDLINE [do] DOI: 10.1107/S2059798317013353

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[PMID]: 28851158 [Au] Autor: Nguyen QA; Cho E; Trinh LTP; Jeong JS; Bae HJ [Ad] Endereço: Bio-energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea. [Ti] Título: Development of an integrated process to produce d-mannose and bioethanol from coffee residue waste. [So] Source: Bioresour Technol;244(Pt 1):1039-1048, 2017 Nov. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A novel, integrated process for economical high-yield production of d-mannose and ethanol from coffee residue waste (CRW), which is abundant and widely available, was reported. The process involves pretreatment, enzymatic hydrolysis, fermentation, color removal, and pervaporation, which can be performed using environmentally friendly technologies. The CRW was pretreated with ethanol at high temperature and then hydrolyzed with enzymes produced in-house to yield sugars. Key points of the process are: manipulations of the fermentation step that allowing bioethanol-producing yeasts to use almost glucose and galactose to produce ethanol, while retaining large amounts of d-mannose in the fermented broth; removal of colored compounds and other components from the fermented broth; and separation of ethanol and d-mannose through pervaporation. Under optimized conditions, approximately 15.7g dry weight (DW) of d-mannose (approximately 46% of the mannose) and approximately 11.3g DW of ethanol from 150g DW of ethanol-pretreated CRW, were recovered. [Mh] Termos MeSH primário: Biocombustíveis Café Manose Saccharomyces cerevisiae [Mh] Termos MeSH secundário: Etanol Fermentação Hidrólise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biofuels); 0 (Coffee); 3K9958V90M (Ethanol); PHA4727WTP (Mannose) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171025 [Lr] Data última revisão: 171025 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170831 [St] Status: MEDLINE

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[PMID]: 28759052 [Au] Autor: Zhang D; Chia C; Jiao X; Jin W; Kasagi S; Wu R; Konkel JE; Nakatsukasa H; Zanvit P; Goldberg N; Chen Q; Sun L; Chen ZJ; Chen W [Ad] Endereço: Mucosal Immunology Section, NIDCR, US National Institutes of Health, Bethesda, Maryland, USA. [Ti] Título: D-mannose induces regulatory T cells and suppresses immunopathology. [So] Source: Nat Med;23(9):1036-1045, 2017 Sep. [Is] ISSN: 1546-170X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: D-mannose, a C-2 epimer of glucose, exists naturally in many plants and fruits, and is found in human blood at concentrations less than one-fiftieth of that of glucose. However, although the roles of glucose in T cell metabolism, diabetes and obesity are well characterized, the function of D-mannose in T cell immune responses remains unknown. Here we show that supraphysiological levels of D-mannose safely achievable by drinking-water supplementation suppressed immunopathology in mouse models of autoimmune diabetes and airway inflammation, and increased the proportion of Foxp3 regulatory T cells (T cells) in mice. In vitro, D-mannose stimulated T cell differentiation in human and mouse cells by promoting TGF-ß activation, which in turn was mediated by upregulation of integrin α ß and reactive oxygen species generated by increased fatty acid oxidation. This previously unrecognized immunoregulatory function of D-mannose may have clinical applications for immunopathology. [Mh] Termos MeSH primário: Colite/imunologia Diabetes Mellitus Tipo 1/imunologia Pneumopatias/imunologia Pulmão/efeitos dos fármacos Manose/farmacologia Pâncreas/efeitos dos fármacos Hipersensibilidade Respiratória/imunologia Linfócitos T Reguladores/efeitos dos fármacos Fator de Crescimento Transformador beta/efeitos dos fármacos [Mh] Termos MeSH secundário: Transferência Adotiva Animais Colo/efeitos dos fármacos Suplementos Nutricionais Modelos Animais de Doenças Ácidos Graxos/metabolismo Citometria de Fluxo Fatores de Transcrição Forkhead/metabolismo Seres Humanos Técnicas In Vitro Inflamação Integrinas/efeitos dos fármacos Integrinas/imunologia Metabolismo dos Lipídeos/efeitos dos fármacos Pulmão/imunologia Pneumopatias/induzido quimicamente Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos NOD Ovalbumina/efeitos adversos Oxirredução/efeitos dos fármacos Pâncreas/imunologia Espécies Reativas de Oxigênio/metabolismo Reação em Cadeia da Polimerase em Tempo Real Hipersensibilidade Respiratória/induzido quimicamente Reação em Cadeia da Polimerase Via Transcriptase Reversa Baço/efeitos dos fármacos Baço/imunologia Linfócitos T Reguladores/imunologia Linfócitos T Reguladores/metabolismo Fator de Crescimento Transformador beta/imunologia Regulação para Cima [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fatty Acids); 0 (Forkhead Transcription Factors); 0 (Foxp3 protein, mouse); 0 (Integrins); 0 (Reactive Oxygen Species); 0 (Transforming Growth Factor beta); 0 (integrin alphavbeta8); 9006-59-1 (Ovalbumin); PHA4727WTP (Mannose) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171002 [Lr] Data última revisão: 171002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170801 [St] Status: MEDLINE [do] DOI: 10.1038/nm.4375

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[PMID]: 28678436 [Au] Autor: Toraskar S; Gade M; Sangabathuni S; Thulasiram HV; Kikkeri R [Ad] Endereço: Department of Chemistry, Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, India. [Ti] Título: Exploring the Influence of Shapes and Heterogeneity of Glyco-Gold Nanoparticles on Bacterial Binding for Preventing Infections. [So] Source: ChemMedChem;12(14):1116-1124, 2017 Jul 20. [Is] ISSN: 1860-7187 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: To investigate the effects of the heterogeneity and shape of glyco-nanoprobes on carbohydrate-protein interactions (CPIs), α-d-mannose- and ß-d-galactose-linked homo- and heterogeneous glycodendrons were synthesized and immobilized on spherical and rod-shaped gold nanoparticles (AuNPs). Lectin and bacterial binding studies of these glyco-AuNPs clearly illustrate that multivalency and shape of AuNPs contribute significantly to CPIs than the heterogeneity of glycodendrons. Finally, bacterial infection of HeLa cells was effectively inhibited by the homogeneous glycodendron-conjugated rod-shaped AuNPs relative to their heterogeneous counterparts. Overall, these results provide insight into the role of AuNP shape and multivalency as potential factors to regulate CPIs. [Mh] Termos MeSH primário: Dendrímeros/química Escherichia coli/efeitos dos fármacos Galactose/química Ouro/química Manose/química Nanopartículas Metálicas/química [Mh] Termos MeSH secundário: Aderência Bacteriana/efeitos dos fármacos Sobrevivência Celular/efeitos dos fármacos Dendrímeros/farmacologia Escherichia coli/fisiologia Células HeLa Seres Humanos Tamanho da Partícula Lectinas de Plantas/química Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Dendrimers); 0 (Plant Lectins); 7440-57-5 (Gold); PHA4727WTP (Mannose); X2RN3Q8DNE (Galactose) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170925 [Lr] Data última revisão: 170925 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170706 [St] Status: MEDLINE [do] DOI: 10.1002/cmdc.201700218

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[PMID]: 28652405 [Au] Autor: Feinberg H; Jégouzo SAF; Rex MJ; Drickamer K; Weis WI; Taylor ME [Ad] Endereço: From the Departments of Structural Biology and Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 and. [Ti] Título: Mechanism of pathogen recognition by human dectin-2. [So] Source: J Biol Chem;292(32):13402-13414, 2017 Aug 11. [Is] ISSN: 1083-351X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Dectin-2, a C-type lectin on macrophages and other cells of the innate immune system, functions in response to pathogens, particularly fungi. The carbohydrate-recognition domain (CRD) in dectin-2 is linked to a transmembrane sequence that interacts with the common Fc receptor γ subunit to initiate immune signaling. The molecular mechanism by which dectin-2 selectively binds to pathogens has been investigated by characterizing the CRD expressed in a bacterial system. Competition binding studies indicated that the CRD binds to monosaccharides with modest affinity and that affinity was greatly enhanced for mannose-linked α1-2 or α1-4 to a second mannose residue. Glycan array analysis confirmed selective binding of the CRD to glycans that contain Manα1-2Man epitopes. Crystals of the CRD in complex with a mammalian-type high-mannose Man GlcNAc oligosaccharide exhibited interaction with Manα1-2Man on two different termini of the glycan, with the reducing-end mannose residue ligated to Ca in a primary binding site and the nonreducing terminal mannose residue occupying an adjacent secondary site. Comparison of the binding sites in DC-SIGN and langerin, two other pathogen-binding receptors of the innate immune system, revealed why these two binding sites accommodate only terminal Manα1-2Man structures, whereas dectin-2 can bind Manα1-2Man in internal positions in mannans and other polysaccharides. The specificity and geometry of the dectin-2-binding site provide the molecular mechanism for binding of dectin-2 to fungal mannans and also to bacterial lipopolysaccharides, capsular polysaccharides, and lipoarabinomannans that contain the Manα1-2Man disaccharide unit. [Mh] Termos MeSH primário: Dissacarídeos/metabolismo Imunidade Inata Lectinas Tipo C/metabolismo Manose/metabolismo Modelos Moleculares Oligossacarídeos/metabolismo Polissacarídeos/metabolismo [Mh] Termos MeSH secundário: Sítios de Ligação Configuração de Carboidratos Cristalografia por Raios X Dissacarídeos/química Epitopos/química Epitopos/metabolismo Escherichia coli/imunologia Escherichia coli/metabolismo Seres Humanos Proteínas Imobilizadas/química Proteínas Imobilizadas/genética Proteínas Imobilizadas/metabolismo Corpos de Inclusão/metabolismo Cinética Lectinas Tipo C/química Lectinas Tipo C/genética Ligantes Manose/química Oligossacarídeos/química Fragmentos de Peptídeos/química Fragmentos de Peptídeos/genética Fragmentos de Peptídeos/metabolismo Filogenia Polissacarídeos/química Conformação Proteica Domínios e Motivos de Interação entre Proteínas Proteínas Recombinantes/química Proteínas Recombinantes/metabolismo [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Disaccharides); 0 (Epitopes); 0 (Immobilized Proteins); 0 (Lectins, C-Type); 0 (Ligands); 0 (Oligosaccharides); 0 (Peptide Fragments); 0 (Polysaccharides); 0 (Recombinant Proteins); 0 (dectin-2, human); PHA4727WTP (Mannose) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170825 [Lr] Data última revisão: 170825 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170628 [St] Status: MEDLINE [do] DOI: 10.1074/jbc.M117.799080

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[PMID]: 28640361 [Au] Autor: Long Y; Sanchez-Espiridion B; Lin M; White L; Mishra L; Raju GS; Kopetz S; Eng C; Hildebrandt MAT; Chang DW; Ye Y; Liang D; Wu X [Ad] Endereço: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas. [Ti] Título: Global and targeted serum metabolic profiling of colorectal cancer progression. [So] Source: Cancer;123(20):4066-4074, 2017 Oct 15. [Is] ISSN: 1097-0142 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Patients with colorectal adenoma polyps (PLPs) are at higher risk for developing colorectal cancer (CRC). However, the development of improved and robust biomarkers to enable the screening, surveillance, and early detection of PLPs and CRC continues to be a challenge. The aim of this study was to identify biomarkers of progression to CRC through metabolomic profiling of human serum samples with a multistage approach. METHODS: Metabolomic profiling was conducted with the Metabolon platform for 30 CRC patients, 30 PLP patients, and 30 control subjects, and this was followed by the targeted validation of the top metabolites in an additional set of 50 CRC patients, 50 PLP patients, and 50 controls with liquid chromatography-tandem mass spectrometry. Unconditional multivariate logistic regression models, adjusted for covariates, were used to evaluate associations with PLP and CRC risk. RESULTS: For the discovery phase, 404 serum metabolites were detected, with 50 metabolites showing differential levels between CRC patients, PLP patients, and controls (P for trend < .05). After validation, the 3 top metabolites (xanthine, hypoxanthine, and d-mannose) were validated: lower levels of xanthine and hypoxanthine and higher levels of d-mannose were found in PLP and CRC cases versus controls. A further exploratory analysis of metabolic pathways revealed key roles for the urea cycle and caffeine metabolism associated with PLP and CRC risk. In addition, a joint effect of the top metabolites with smoking and a significant interaction with the body mass index were observed. An analysis of the ratio of hypoxanthine levels to xanthine levels indicated an association with CRC progression. CONCLUSIONS: These results suggest the potential utility of circulating metabolites as novel biomarkers for the early detection of CRC. Cancer 2017;123:4066-74. © 2017 American Cancer Society. [Mh] Termos MeSH primário: Adenoma/sangue Pólipos do Colo/sangue Neoplasias Colorretais/sangue [Mh] Termos MeSH secundário: Adenoma/metabolismo Adulto Idoso Cafeína/metabolismo Estudos de Casos e Controles Cromatografia Líquida Pólipos do Colo/metabolismo Neoplasias Colorretais/metabolismo Progressão da Doença Feminino Seres Humanos Hipoxantina/sangue Pólipos Intestinais/sangue Pólipos Intestinais/metabolismo Modelos Logísticos Masculino Manose/sangue Metabolômica Meia-Idade Análise Multivariada Espectrometria de Massas em Tandem Ureia/metabolismo Xantina/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 1AVZ07U9S7 (Xanthine); 2TN51YD919 (Hypoxanthine); 3G6A5W338E (Caffeine); 8W8T17847W (Urea); PHA4727WTP (Mannose) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171011 [Lr] Data última revisão: 171011 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170623 [St] Status: MEDLINE [do] DOI: 10.1002/cncr.30829

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[PMID]: 28601009 [Au] Autor: Chiara Pietrogrande M; Barbaro E; Bove MC; Clauser G; Colombi C; Corbella L; Cuccia E; Dalla Torre S; Decesari S; Fermo P; Gambaro A; Gianelle V; Ielpo P; Larcher R; Lazzeri P; Massabò D; Melchionna G; Nardin T; Paglione M; Perrino C; Prati P; Visentin M; Zanca N; Zangrando R [Ad] Endereço: Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy. Electronic address: mpc@unife.it. [Ti] Título: Results of an interlaboratory comparison of analytical methods for quantification of anhydrosugars and biosugars in atmospheric aerosol. [So] Source: Chemosphere;184:269-277, 2017 Oct. [Is] ISSN: 1879-1298 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: An interlaboratory comparison was performed to evaluate the analytical methods for quantification of anhydrosugars - levoglucosan, mannosan, galactosan - and biosugars - arabitol, glucose and mannitol - in atmospheric aerosol. The performance of 10 laboratories in Italy currently involved in such analyses was investigated on twenty-six PM (particulate matter) ambient filters, three synthetic PM filters and three aqueous standard solutions. An acceptable interlaboratory variability was found, determined as the mean relative standard deviation (RSD%) of the results from the participating laboratories, with the mean RSD% values ranging from 25% to 46% and decreasing with increasing sugar concentration. The investigated methods show good accuracy, evaluated as the percentage error (ε%) related to mean values, since method biases ranged within ±20% for most of the analytes measured in the different laboratories. The detailed investigation (ANOVA analysis at p < 0.05) of the contribution of each laboratory to the total variability and the measurement accuracy shows that comparable results are generated by the different methods, despite the great diversity in terms of extraction conditions, chromatographic separation - more recent LC (liquid chromatography) and EC (exchange chromatography) methods compared to more widespread GC (gas chromatography) - and detection systems, namely PAD (pulsed amperometric detection) or mass spectrometry. [Mh] Termos MeSH primário: Aerossóis/análise Poluentes Atmosféricos/análise Carboidratos/análise Monitoramento Ambiental/métodos Espectrometria de Massas/métodos Variações Dependentes do Observador [Mh] Termos MeSH secundário: Cromatografia Líquida Galactose/análogos & derivados Galactose/análise Cromatografia Gasosa-Espectrometria de Massas/métodos Glucose/análogos & derivados Glucose/análise Itália Manose/análogos & derivados Manose/análise Material Particulado/análise Álcoois Açúcares/análise [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aerosols); 0 (Air Pollutants); 0 (Carbohydrates); 0 (Particulate Matter); 0 (Sugar Alcohols); 0 (galactosan); 0 (mannosan); 5132N17FSD (1,6-anhydro-beta-glucopyranose); IY9XDZ35W2 (Glucose); PHA4727WTP (Mannose); X2RN3Q8DNE (Galactose); YFV05Y57M9 (arabitol) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170611 [St] Status: MEDLINE

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