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  1 / 163 MEDLINE  
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[PMID]:27552923
[Au] Autor:Tamari Z; Yona AH; Pilpel Y; Barkai N
[Ad] Endereço:Department of molecular genetics, Weizmann institute of science, Rehovot, 76100, Israel. zvit2468@gmail.com.
[Ti] Título:Rapid evolutionary adaptation to growth on an 'unfamiliar' carbon source.
[So] Source:BMC Genomics;17:674, 2016 08 24.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cells constantly adapt to changes in their environment. When environment shifts between conditions that were previously encountered during the course of evolution, evolutionary-programmed responses are possible. Cells, however, may also encounter a new environment to which a novel response is required. To characterize the first steps in adaptation to a novel condition, we studied budding yeast growth on xylulose, a sugar that is very rarely found in the wild. RESULTS: We previously reported that growth on xylulose induces the expression of amino acid biosynthesis genes in multiple natural yeast isolates. This induction occurs despite the presence of amino acids in the growth medium and is a unique response to xylulose, not triggered by naturally available carbon sources. Propagating these strains for ~300 generations on xylulose significantly improved their growth rate. Notably, the most significant change in gene expression was the loss of amino acid biosynthesis gene induction. Furthermore, the reduction in amino-acid biosynthesis gene expression on xylulose was tightly correlated with the improvement in growth rate, suggesting that internal depletion of amino-acids presented a major bottleneck limiting growth in xylulose. CONCLUSIONS: We discuss the possible implications of our results for explaining how cells maintain the balance between supply and demand of amino acids during growth in evolutionary 'familiar' vs. 'novel' conditions.
[Mh] Termos MeSH primário: Carbono/metabolismo
Proteínas Fúngicas/metabolismo
Saccharomycetales/crescimento & desenvolvimento
Xilulose/metabolismo
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Aminoácidos/biossíntese
Regulação Fúngica da Expressão Gênica
Saccharomycetales/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Amino Acids); 0 (Fungal Proteins); 5962-29-8 (Xylulose); 7440-44-0 (Carbon)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-3010-x


  2 / 163 MEDLINE  
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[PMID]:27166648
[Au] Autor:Gao H; Khera E; Lee JK; Wen F
[Ad] Endereço:Department of Chemical Engineering, Konkuk University.
[Ti] Título:Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability.
[So] Source:J Vis Exp;(110), 2016 Apr 22.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have recently developed a simple, reusable and coupled whole-cell biocatalytic system with the capability of cofactor regeneration and biocatalyst immobilization for improved production yield and sustained synthesis. Described herewith is the experimental procedure for the development of such a system consisting of two E. coli strains that express functionally complementary enzymes. Together, these two enzymes can function co-operatively to mediate the regeneration of expensive cofactors for improving the product yield of the bioreaction. In addition, the method of synthesizing an immobilized form of the coupled biocatalytic system by encapsulation of whole cells in calcium alginate beads is reported. As an example, we present the improved biosynthesis of L-xylulose from L-arabinitol by coupling E. coli cells expressing the enzymes L-arabinitol dehydrogenase or NADH oxidase. Under optimal conditions and using an initial concentration of 150 mM L-arabinitol, the maximal L-xylulose yield reached 96%, which is higher than those reported in the literature. The immobilized form of the coupled whole-cell biocatalysts demonstrated good operational stability, maintaining 65% of the yield obtained in the first cycle after 7 cycles of successive re-use, while the free cell system almost completely lost the catalytic activity. Therefore, the methods reported here provides two strategies that could help improve the industrial production of L-xylulose, as well as other value-added compounds requiring the use of cofactors in general.
[Mh] Termos MeSH primário: Alginatos/química
Coenzimas/metabolismo
Enzimas Imobilizadas/química
[Mh] Termos MeSH secundário: Biocatálise
Coenzimas/química
Enzimas Imobilizadas/metabolismo
Escherichia coli/enzimologia
Escherichia coli/metabolismo
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Desidrogenase do Álcool de Açúcar/química
Desidrogenase do Álcool de Açúcar/metabolismo
Xilulose/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Alginates); 0 (Coenzymes); 0 (Enzymes, Immobilized); 0 (Hexuronic Acids); 5962-29-8 (Xylulose); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); EC 1.1.- (Sugar Alcohol Dehydrogenases); EC 1.1.1.12 (L-arabinitol 4-dehydrogenase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160512
[St] Status:MEDLINE
[do] DOI:10.3791/53944


  3 / 163 MEDLINE  
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[PMID]:26975753
[Au] Autor:Qi XH; Zhu JF; Yun JH; Lin J; Qi YL; Guo Q; Xu H
[Ad] Endereço:School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: qxh@ujs.edu.cn.
[Ti] Título:Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell.
[So] Source:J Biosci Bioeng;122(3):257-62, 2016 Sep.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Xylitol has numerous applications in food and pharmaceutical industry, and it can be biosynthesized by microorganisms. In the present study, xdh gene, encoding xylitol dehydrogenase (XDH), was cloned from the genome of Gluconobacter oxydans CGMCC 1.49 and overexpressed in Escherichia coli BL21. Sequence analysis revealed that XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/reductase family. And then, the enzymatic properties and kinetic parameter of purified recombinant XDH were investigated. Subsequently, transformations of xylitol from d-xylulose and d-arabitol, respectively, were studied through mixed culture of resting cells of G. oxydans wild-type strain and recombinant strain BL21-xdh. We obtained 28.80 g/L xylitol by mixed culture from 30 g/L d-xylulose in 28 h. The production was increased by more than three times as compared with that of wild-type strain. Furthermore, 25.10 g/L xylitol was produced by the mixed culture from 30 g/L d-arabitol in 30 h with a yield of 0.837 g/g, and the max volumetric productivity of 0.990 g/L h was obtained at 22 h. These contrast to the fact that wild-type strain G. oxydans only produced 8.10 g/L xylitol in 30 h with a yield of 0.270 g/g. To our knowledge, these values are the highest among the reported yields and productivity efficiencies of xylitol from d-arabitol with engineering strains.
[Mh] Termos MeSH primário: D-Xilulose Redutase/genética
D-Xilulose Redutase/metabolismo
Escherichia coli/metabolismo
Gluconobacter oxydans/enzimologia
Engenharia Metabólica
Xilitol/biossíntese
[Mh] Termos MeSH secundário: Clonagem Molecular
D-Xilulose Redutase/química
D-Xilulose Redutase/isolamento & purificação
Escherichia coli/genética
Gluconobacter oxydans/genética
Cinética
NAD/metabolismo
Álcoois Açúcares/metabolismo
Xilulose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sugar Alcohols); 0U46U6E8UK (NAD); 5962-29-8 (Xylulose); EC 1.1.1.9 (D-Xylulose Reductase); VCQ006KQ1E (Xylitol); YFV05Y57M9 (arabitol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE


  4 / 163 MEDLINE  
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[PMID]:26526452
[Au] Autor:Meng Q; Zhang T; Jiang B; Mu W; Miao M
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
[Ti] Título:Advances in applications, metabolism, and biotechnological production of L-xylulose.
[So] Source:Appl Microbiol Biotechnol;100(2):535-40, 2016 Jan.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:L-Xylulose is an intermediate in certain metabolic pathways and is classified as a rare sugar. It shows important physiological effects such as acting as an inhibitor of α-glucosidase and decreasing blood glucose, and it can be employed to produce other significant rare sugars, such as L-ribose and L-xylose which contribute to the production of antiviral drugs. Chemical synthesis of L-xylulose was performed, but it is difficult and low yielding. The biotransformation from xylitol to L-xylulose by xylitol 4-dehydrogenase was studied intensively. This review describes the occurrence of L-xylulose in certain metabolic pathways, its bioproduction, and application potential.
[Mh] Termos MeSH primário: Biotecnologia/métodos
D-Xilulose Redutase/metabolismo
Redes e Vias Metabólicas
Xilulose/metabolismo
[Mh] Termos MeSH secundário: Antivirais
Biotecnologia/tendências
Biotransformação
Glucose/metabolismo
Ribose/metabolismo
Desidrogenase do Álcool de Açúcar/metabolismo
Álcoois Açúcares/metabolismo
Xilitol/metabolismo
Xilose/metabolismo
Xilulose/síntese química
Xilulose/farmacologia
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Sugar Alcohols); 5962-29-8 (Xylulose); 681HV46001 (Ribose); A1TA934AKO (Xylose); EC 1.1.- (Sugar Alcohol Dehydrogenases); EC 1.1.1.9 (D-Xylulose Reductase); EC 3.2.1.20 (alpha-Glucosidases); IY9XDZ35W2 (Glucose); VCQ006KQ1E (Xylitol)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151104
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-015-7087-y


  5 / 163 MEDLINE  
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[PMID]:26186096
[Au] Autor:Cam Y; Alkim C; Trichez D; Trebosc V; Vax A; Bartolo F; Besse P; François JM; Walther T
[Ad] Endereço:INSA, UPS, INP, LISBP, Université de Toulouse , 135 Avenue de Rangueil, 31077 Toulouse, France.
[Ti] Título:Engineering of a Synthetic Metabolic Pathway for the Assimilation of (d)-Xylose into Value-Added Chemicals.
[So] Source:ACS Synth Biol;5(7):607-18, 2016 07 15.
[Is] ISSN:2161-5063
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A synthetic pathway for (d)-xylose assimilation was stoichiometrically evaluated and implemented in Escherichia coli strains. The pathway proceeds via isomerization of (d)-xylose to (d)-xylulose, phosphorylation of (d)-xylulose to obtain (d)-xylulose-1-phosphate (X1P), and aldolytic cleavage of the latter to yield glycolaldehyde and DHAP. Stoichiometric analyses showed that this pathway provides access to ethylene glycol with a theoretical molar yield of 1. Alternatively, both glycolaldehyde and DHAP can be converted to glycolic acid with a theoretical yield that is 20% higher than for the exclusive production of this acid via the glyoxylate shunt. Simultaneous expression of xylulose-1 kinase and X1P aldolase activities, provided by human ketohexokinase-C and human aldolase-B, respectively, restored growth of a (d)-xylulose-5-kinase mutant on xylose. This strain produced ethylene glycol as the major metabolic endproduct. Metabolic engineering provided strains that assimilated the entire C2 fraction into the central metabolism or that produced 4.3 g/L glycolic acid at a molar yield of 0.9 in shake flasks.
[Mh] Termos MeSH primário: Escherichia coli/metabolismo
Engenharia Metabólica/métodos
Xilose/metabolismo
[Mh] Termos MeSH secundário: Acetaldeído/análogos & derivados
Acetaldeído/metabolismo
Aldeído Liases/genética
Aldeído Liases/metabolismo
Fosfato de Di-Hidroxiacetona/genética
Fosfato de Di-Hidroxiacetona/metabolismo
Enzimas/genética
Enzimas/metabolismo
Escherichia coli/genética
Glicolatos/metabolismo
Mutação
Pentosefosfatos/genética
Pentosefosfatos/metabolismo
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
Xilose/genética
Xilulose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzymes); 0 (Glycolates); 0 (Pentosephosphates); 0WT12SX38S (glycolic acid); 2547-08-2 (xylulose-1-phosphate); 57-04-5 (Dihydroxyacetone Phosphate); 5962-29-8 (Xylulose); A1TA934AKO (Xylose); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 4.1.2.- (Aldehyde-Lyases); GO1N1ZPR3B (Acetaldehyde); W0A0XPU08U (glycolaldehyde)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150718
[St] Status:MEDLINE
[do] DOI:10.1021/acssynbio.5b00103


  6 / 163 MEDLINE  
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[PMID]:26311112
[Au] Autor:Chang HH; Chao HN; Walker CS; Choong SY; Phillips A; Loomes KM
[Ad] Endereço:School of Biological Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.
[Ti] Título:Renal depletion of myo-inositol is associated with its increased degradation in animal models of metabolic disease.
[So] Source:Am J Physiol Renal Physiol;309(9):F755-63, 2015 Nov 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Renal depletion of myo-inositol (MI) is associated with the pathogenesis of diabetic nephropathy in animal models, but the underlying mechanisms involved are unclear. We hypothesized that MI depletion was due to changes in inositol metabolism and therefore examined the expression of genes regulating de novo biosynthesis, reabsorption, and catabolism of MI. We also extended the analyses from diabetes mellitus to animal models of dietary-induced obesity and hypertension. We found that renal MI depletion was pervasive across these three distinct disease states in the relative order: hypertension (-51%)>diabetes mellitus (-35%)>dietary-induced obesity (-19%). In 4-wk diabetic kidneys and in kidneys derived from insulin-resistant and hypertensive rats, MI depletion was correlated with activity of the MI-degrading enzyme myo-inositol oxygenase (MIOX). By contrast, there was decreased MIOX expression in 8-wk diabetic kidneys. Immunohistochemistry localized the MI-degrading pathway comprising MIOX and the glucuronate-xylulose (GX) pathway to the proximal tubules within the renal cortex. These findings indicate that MI depletion could reflect increased catabolism through MIOX and the GX pathway and implicate a common pathological mechanism contributing to renal oxidative stress in metabolic disease.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/metabolismo
Hipertensão/metabolismo
Inositol/metabolismo
Túbulos Renais Proximais/metabolismo
Obesidade/metabolismo
[Mh] Termos MeSH secundário: Animais
Diabetes Mellitus Experimental/complicações
Diabetes Mellitus Experimental/genética
Nefropatias Diabéticas/etiologia
Nefropatias Diabéticas/metabolismo
Hipertensão/complicações
Hipertensão/genética
Inositol/deficiência
Inositol Oxigenase/genética
Inositol Oxigenase/metabolismo
Resistência à Insulina
Túbulos Renais Proximais/enzimologia
Masculino
Camundongos Endogâmicos C57BL
Obesidade/complicações
Obesidade/genética
Proteínas/genética
Proteínas/metabolismo
Ratos Endogâmicos SHR
Ratos Endogâmicos WKY
Ratos Wistar
Xilulose/genética
Xilulose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proteins); 4L6452S749 (Inositol); 5962-29-8 (Xylulose); EC 1.13.99.1 (Inositol Oxygenase); EC 1.13.99.1 (Miox protein, rat)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:151102
[Lr] Data última revisão:
151102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150828
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00164.2015


  7 / 163 MEDLINE  
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[PMID]:26194109
[Au] Autor:Liu Y; Rainey PB; Zhang XX
[Ad] Endereço:Institute of Natural and Mathematical Sciences, Massey University, Auckland, 0745, New Zealand.
[Ti] Título:Molecular mechanisms of xylose utilization by Pseudomonas fluorescens: overlapping genetic responses to xylose, xylulose, ribose and mannitol.
[So] Source:Mol Microbiol;98(3):553-70, 2015 Oct.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial degradation of xylose is sequentially mediated by two enzymes - an isomerase (XutA) and a xylulokinase (XutB) - with xylulose as an intermediate. Pseudomonas fluorescens SBW25, though capable of growth on xylose as a sole carbon source, encodes only one degradative enzyme XutA at the xylose utilization (xut) locus. Here, using site-directed mutagenesis and transcriptional assays, we have identified two functional xylulokinase-encoding genes (xutB1 and xutB2) and further show that expression of xutB1 is specifically induced by xylose. Surprisingly, xylose-induced xutB1 expression is mediated by the mannitol-responsive regulator MtlR, using xylulose rather than xylose as the direct inducer. In contrast, expression of the xutA operon is regulated by XutR - a transcriptional activator of the AraC family - in a xylose-, xylulose- and ribose-dependent manner. Detailed genetic and biochemical analyses of XutR, including DNase I footprinting assays, suggest an unconventional model of XutR regulation that does not involve DNA-looping, a mechanism typically found for AraC-type regulators from enteric bacteria. XutR functions as a dimer and recognizes two inverted repeat sequences, but binding to one half site is weak thus requiring an inducer molecule such as xylose for activation.
[Mh] Termos MeSH primário: Manitol/metabolismo
Pentoses/metabolismo
Pseudomonas fluorescens/genética
Pseudomonas fluorescens/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Regulação Bacteriana da Expressão Gênica
Genoma Bacteriano
Dados de Sequência Molecular
Mutagênese Sítio-Dirigida
Óperon
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
Ribose/metabolismo
Xilose/metabolismo
Xilulose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pentoses); 3OWL53L36A (Mannitol); 5962-29-8 (Xylulose); 681HV46001 (Ribose); A1TA934AKO (Xylose); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.17 (xylulokinase)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151027
[Lr] Data última revisão:
151027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150722
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13142


  8 / 163 MEDLINE  
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[PMID]:25791332
[Au] Autor:Hohenschuh W; Hector R; Murthy GS
[Ad] Endereço:Oregon State University, United States.
[Ti] Título:A dynamic flux balance model and bottleneck identification of glucose, xylose, xylulose co-fermentation in Saccharomyces cerevisiae.
[So] Source:Bioresour Technol;188:153-60, 2015.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further experiments showed xylulose transport through the HXT family of non-specific glucose transporters. A genome scale flux balance model was developed which included an improved variable sugar uptake constraint controlled by HXT expression. Model predictions closely matched experimental xylulose utilization rates suggesting the combination of transport and xylulokinase constraints is sufficient to explain xylulose utilization limitation in S. cerevisiae.
[Mh] Termos MeSH primário: Glucose/química
Saccharomyces cerevisiae/metabolismo
Xilose/química
Xilulose/química
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/química
Celulose/química
Etanol/química
Fermentação
Genótipo
Microbiologia Industrial
Método de Monte Carlo
Fosfotransferases (Aceptor do Grupo Álcool)/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3K9958V90M (Ethanol); 5962-29-8 (Xylulose); 8L70Q75FXE (Adenosine Triphosphate); 9004-34-6 (Cellulose); A1TA934AKO (Xylose); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.17 (xylulokinase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150508
[Lr] Data última revisão:
150508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150321
[St] Status:MEDLINE


  9 / 163 MEDLINE  
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[PMID]:25769691
[Au] Autor:Patel A; Pruthi V; Singh RP; Pruthi PA
[Ad] Endereço:Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
[Ti] Título:Synergistic effect of fermentable and non-fermentable carbon sources enhances TAG accumulation in oleaginous yeast Rhodosporidium kratochvilovae HIMPA1.
[So] Source:Bioresour Technol;188:136-44, 2015.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Novel strategy for enhancing TAG accumulation by simultaneous utilization of fermentable and non-fermentable carbon sources as substrate for cultivation of oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 were undertaken in this investigation. The yeast strain showed direct correlation between the size of lipid bodies, visualized by BODIPY stain (493-515 nm) and TAG accumulation when examined on individual fermenting and non-fermenting carbon sources and their mixtures. Maximum TAG accumulation (µm) in glucose (2.38 ± 0.52), fructose (4.03 ± 0.38), sucrose (4.24 ± 0.45), glycerol (4.35 ± 0.54), xylulose (3.94 ± 0.12), and arabinose (2.98 ± 0.43) were observed. Synergistic effect of the above carbon sources (fermentable and non-fermentable) in equimolar concentration revealed maximum lipid droplet size of 5.35 ± 0.76 µm and cell size of 6.89 ± 0.97 µm. Total lipid content observed in mixed carbon sources was 9.26 g/l compared to glucose (6.2g/l). FAME profile revealed enhanced longer chain (C14:0-C24:0) fatty acids in mix carbon sources.
[Mh] Termos MeSH primário: Basidiomycota/química
Carbono/química
Fermentação
Triglicerídeos/química
[Mh] Termos MeSH secundário: Arabinose/química
Biomassa
Reatores Biológicos
Ácidos Graxos/química
Frutose/química
Cromatografia Gasosa-Espectrometria de Massas
Glucose/química
Glicerol/química
Microbiologia Industrial/métodos
Gotículas Lipídicas/química
Lipídeos/química
Microscopia de Fluorescência
Saccharomyces cerevisiae
Software
Sacarose/química
Xilulose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Lipids); 0 (Triglycerides); 30237-26-4 (Fructose); 57-50-1 (Sucrose); 5962-29-8 (Xylulose); 7440-44-0 (Carbon); B40ROO395Z (Arabinose); IY9XDZ35W2 (Glucose); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150508
[Lr] Data última revisão:
150508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150315
[St] Status:MEDLINE


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[PMID]:25577107
[Au] Autor:Gao DM; Kobayashi T; Adachi S
[Ad] Endereço:Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
[Ti] Título:Production of rare sugars from common sugars in subcritical aqueous ethanol.
[So] Source:Food Chem;175:465-70, 2015 May 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new isomerization reaction was developed to synthesize rare ketoses. D-tagatose, D-xylulose, and D-ribulose were obtained in the maximum yields of 24%, 38%, and 40%, respectively, from the corresponding aldoses, D-galactose, D-xylose, and D-ribose, by treating the aldoses with 80% (v/v) subcritical aqueous ethanol at 180°C. The maximum productivity of D-tagatose was ca. 80 g/(Lh). Increasing the concentration of ethanol significantly increased the isomerization of D-galactose. Variation in the reaction temperature did not significantly affect the production of D-tagatose from D-galactose. Subcritical aqueous ethanol converted both 2,3-threo and 2,3-erythro aldoses to the corresponding C-2 ketoses in high yields. Thus, the treatment of common aldoses in subcritical aqueous ethanol can be regarded as a new method to synthesize the corresponding rare sugars.
[Mh] Termos MeSH primário: Carboidratos/química
Etanol/química
Cetoses/síntese química
[Mh] Termos MeSH secundário: Carboidratos/síntese química
Hexoses/química
Pentoses/química
Xilulose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Hexoses); 0 (Ketoses); 0 (Pentoses); 3K9958V90M (Ethanol); 5556-48-9 (ribulose); 5962-29-8 (Xylulose); T7A20Y888Y (tagatose)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150112
[St] Status:MEDLINE



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