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[PMID]:29296106
[Au] Autor:Seifert JG; Brumet A; St Cyr JA
[Ad] Endereço:Movement Science Laboratory, Montana State University, Bozeman, MT USA.
[Ti] Título:The influence of D-ribose ingestion and fitness level on performance and recovery.
[So] Source:J Int Soc Sports Nutr;14:47, 2017.
[Is] ISSN:1550-2783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Skeletal muscle adenosine triphosphate (ATP) levels are severely depleted during and following prolonged high intensity exercise. Recovery from these lower ATP levels can take days, which can affect performance on subsequent days of exercise. Untrained individuals often suffer the stress and consequences of acute, repeated bouts of exercise by not having the ability to perform or recovery sufficiently to exercise on subsequent days. Conversely, trained individuals may be able to recover more quickly due to their enhanced metabolic systems. D-Ribose (DR) has been shown to enhance the recovery in ATP; however, it is not known if recovery and performance can be benefitted with DR ingestion. Therefore, this study was designed to determine what influence DR might have on muscular performance, recovery, and metabolism during and following a multi-day exercise regimen. Methods: The study was a double blind, crossover study in 26 healthy subjects compared 10 g/day of DR to 10 g/day of dextrose (DEX, control). All subjects completed 2 days of loading with either DR or DEX, followed by 3 additional days of supplementation and during these 3 days of supplementation, each subject underwent 60 min of high intensity interval exercise in separate daily sessions, which involved cycling (8 min of exercise at 60% and 2 min at 80% VO max), followed by a 2 min power output (PO) test. Subjects were divided into two groups based on peak VO results, lower VO (LVO ) and higher peak VO (HVO ). Results: Mean and peak PO increased significantly from day 1 to day 3 for the DR trial compared to DEX in the LVO group. Rate of perceived exertion (RPE) and creatine kinase (CK) were significantly lower for DR than DEX in the LVO group. No differences in PO, RPE, heart rate, CK, blood urea nitrogen, or glucose were found between either supplement for the HVO group. Conclusion: DR supplementation in the lower VO max group resulted in maintenance in exercise performance, as well as lower levels of RPE and CK. Unlike no observed benefits with DEX supplementation.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Limiar Anaeróbio/efeitos dos fármacos
Desempenho Atlético/fisiologia
Suplementos Nutricionais
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Aptidão Física/fisiologia
Ribose/farmacologia
[Mh] Termos MeSH secundário: Adulto
Limiar Anaeróbio/fisiologia
Estudos Cross-Over
Método Duplo-Cego
Metabolismo Energético/efeitos dos fármacos
Feminino
Seres Humanos
Masculino
Fenômenos Fisiológicos da Nutrição Esportiva
[Pt] Tipo de publicação:CLINICAL TRIAL; COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
681HV46001 (Ribose); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1186/s12970-017-0205-8


  2 / 2907 MEDLINE  
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[PMID]:29337564
[Au] Autor:Bruening EM; Schauss J; Siebert T; Fingerhut BP; Elsaesser T
[Ad] Endereço:Max-Born-Institut für Nichtlineare Optik und Kurzzeitspektroskopie , Max-Born-Str. 2a, D-12489 Berlin, Germany.
[Ti] Título:Vibrational Dynamics and Couplings of the Hydrated RNA Backbone: A Two-Dimensional Infrared Study.
[So] Source:J Phys Chem Lett;9(3):583-587, 2018 Feb 01.
[Is] ISSN:1948-7185
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The equilibrium structure of the RNA sugar-phosphate backbone and its hydration shell is distinctly different from hydrated DNA. Applying femtosecond two-dimensional infrared (2D-IR) spectroscopy in a range from 950 to 1300 cm , we elucidate the character, dynamics, and couplings of backbone modes of a double-stranded RNA A-helix geometry in its aqueous environment. The 2D-IR spectra display a greater number of backbone modes than for DNA, with distinctly different lineshapes of diagonal peaks. Phosphate-ribose interactions and local hydration structures are reflected in the complex coupling pattern of RNA modes. Interactions with the fluctuating water shell give rise to spectral diffusion on a 300 fs time scale, leading to a quasi-homogeneous line shape of the symmetric (PO ) stretching mode of the strongly hydrated phosphate groups. The RNA results are benchmarked by 2D-IR spectra of DNA oligomers in water and analyzed by molecular dynamics and quantum mechanical molecular mechanics simulations.
[Mh] Termos MeSH primário: Simulação de Dinâmica Molecular
RNA/química
Vibração
[Mh] Termos MeSH secundário: DNA/química
Fosfatos/química
Ácidos Fosfóricos
Ribose/metabolismo
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphates); 0 (Phosphoric Acids); 059QF0KO0R (Water); 63231-63-0 (RNA); 681HV46001 (Ribose); 9007-49-2 (DNA); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jpclett.7b03314


  3 / 2907 MEDLINE  
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[PMID]:29280631
[Au] Autor:Zhang N; Tu Z; Wang H; Liu G; Wang Z; Huang T; Qin X; Xie X; Wang A
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang, Jiangxi 330047, China.
[Ti] Título:Liquid Chromatography High-Resolution Mass Spectrometry Identifies the Glycation Sites of Bovine Serum Albumin Induced by d-Ribose with Ultrasonic Treatment.
[So] Source:J Agric Food Chem;66(3):563-570, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ultrasonication is an emerging technology applied in food processing and biological experimental pretreatments. Cavitation phenomena induced during ultrasonic treatment can generate localized high temperature and pressure, which can result in glycation reaction between protein and reducing sugars. In this study, the mixture of bovine serum albumin (BSA) and d-ribose was treated under 600 W for different times. Interestingly, a large amount of carbonized black materials appeared after ultrasonication, while the UV absorbance and intrinsic fluorescence spectra reflecting conformational changes were not obvious. Only 12 sites (11 lysines and 1 arginine) of the BSA with ribose under ultrasonic treatment for 35 min were identified through liquid chromatography high-resolution mass spectrometry (LCHR-MS). K547, K548, R359/R360, and K587 were the most reactive glycated sites, with the average degree of substitution per peptide molecule (DSP) value ranging from 15 to 35%. The glycated modification was distributed not only in domain III, but also in domains I and II. The glycated modification could occur during ultrasonic treatment, thereby influencing the properties of biomacromolecule after extraction.
[Mh] Termos MeSH primário: Ribose/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Animais
Cromatografia Líquida
Glicosilação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Ultrassom
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
27432CM55Q (Serum Albumin, Bovine); 681HV46001 (Ribose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04578


  4 / 2907 MEDLINE  
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[PMID]:28726641
[Au] Autor:Nitzsche R; Günay-Esiyok Ö; Tischer M; Zagoriy V; Gupta N
[Ad] Endereço:From the Department of Molecular Parasitology, Humboldt University, 10115 Berlin, Germany and.
[Ti] Título:A plant/fungal-type phosphoenolpyruvate carboxykinase located in the parasite mitochondrion ensures glucose-independent survival of .
[So] Source:J Biol Chem;292(37):15225-15239, 2017 Sep 15.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:is considered to be one of the most successful intracellular pathogens, because it can reproduce in varied nutritional milieus, encountered in diverse host cell types of essentially any warm-blooded organism. Our earlier work demonstrated that the acute (tachyzoite) stage of depends on cooperativity of glucose and glutamine catabolism to meet biosynthetic demands. Either of these two nutrients can sustain the parasite survival; however, what determines the metabolic plasticity has not yet been resolved. Here, we reveal two discrete phosphoenolpyruvate carboxykinase (PEPCK) enzymes in the parasite, one of which resides in the i ochondrion ( PEPCK ), whereas the other protein is ot xpressed in achyzoites ( PEPCK ). Parasites with an intact glycolysis can tolerate genetic deletions of PEPCK as well as of PEPCK , indicating their nonessential roles for tachyzoite survival. PEPCK can also be ablated in a glycolysis-deficient mutant, while PEPCK is refractory to deletion. Consistent with this, the lytic cycle of a conditional mutant of PEPCK in the glycolysis-impaired strain was aborted upon induced repression of the mitochondrial isoform, demonstrating its essential role for the glucose-independent survival of parasites. Isotope-resolved metabolomics of the conditional mutant revealed defective flux of glutamine-derived carbon into RNA-bound ribose sugar as well as metabolites associated with gluconeogenesis, entailing a critical nodal role of PEPCK in linking catabolism of glucose and glutamine with anabolic pathways. Our data also suggest a homeostatic function of PEPCK in cohesive operation of glycolysis and the tricarboxylic acid cycle in a normal glucose-replete milieu. Conversely, we found that the otherwise integrative enzyme pyruvate carboxylase ( PyC) is dispensable not only in glycolysis-competent but also in glycolysis-deficient tachyzoites despite a mitochondrial localization. Last but not least, the observed physiology of tachyzoites appears to phenocopy cancer cells, which holds promise for developing common therapeutics against both threats.
[Mh] Termos MeSH primário: Mitocôndrias/enzimologia
Modelos Biológicos
Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo
Proteínas de Protozoários/metabolismo
Toxoplasma/metabolismo
[Mh] Termos MeSH secundário: Ciclo do Ácido Cítrico
Deleção de Genes
Gluconeogênese
Glucose/metabolismo
Glutamina/metabolismo
Glicólise
Homeostase
Isoenzimas/genética
Isoenzimas/metabolismo
Metabolômica/métodos
Viabilidade Microbiana
Microscopia de Fluorescência
Mitocôndrias/metabolismo
Mutação
Fosfoenolpiruvato Carboxiquinase (ATP)/genética
Piruvato Carboxilase/genética
Piruvato Carboxilase/metabolismo
Proteínas Recombinantes de Fusão
Ribose/biossíntese
Toxoplasma/citologia
Toxoplasma/crescimento & desenvolvimento
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Protozoan Proteins); 0 (Recombinant Fusion Proteins); 0RH81L854J (Glutamine); 681HV46001 (Ribose); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)); EC 6.4.1.1 (Pyruvate Carboxylase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.802702


  5 / 2907 MEDLINE  
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[PMID]:28716494
[Au] Autor:Avanzo RE; Padrón JM; D'Accorso NB; Fascio ML
[Ad] Endereço:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Buenos Aires, Argentina.
[Ti] Título:Synthesis and in vitro antiproliferative activities of (5-aryl-1,2,4-oxadiazole-3-yl) methyl d-ribofuranosides.
[So] Source:Bioorg Med Chem Lett;27(16):3674-3677, 2017 08 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The emergence of multidrug resistance cell lines is one of the major obstacles in the success of cancer chemotherapeutic treatment. Therefore, it remains a big challenge the development of new and effective drugs to defeat cancer. The presence of nitrogen heterocycles in the architectural design of drugs has led to the discovery of new leading compounds. Herein, we report the synthesis, characterization and in vitro antiproliferative activity against six cancer cell lines of d-ribofuranoside derivatives bearing a 1,2,4-oxadiazolic ring, with the aim of developing new active compounds. Most of these derivatives exhibit significant antiproliferative activities in the micromolar range. Noteworthy, the most potent compound of the series showed better selectivity towards the more resistant colon cancer cell line WiDr.
[Mh] Termos MeSH primário: Antineoplásicos/síntese química
Antineoplásicos/farmacologia
Oxidiazóis/síntese química
Ribose/análogos & derivados
[Mh] Termos MeSH secundário: Antineoplásicos/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Oxidiazóis/química
Oxidiazóis/farmacologia
Ribose/síntese química
Ribose/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Oxadiazoles); 681HV46001 (Ribose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE


  6 / 2907 MEDLINE  
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[PMID]:28504680
[Au] Autor:Dai Q; Moshitch-Moshkovitz S; Han D; Kol N; Amariglio N; Rechavi G; Dominissini D; He C
[Ad] Endereço:Department of Chemistry, The University of Chicago, Chicago, Illinois, USA.
[Ti] Título:Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.
[So] Source:Nat Methods;14(7):695-698, 2017 Jul.
[Is] ISSN:1548-7105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
[Mh] Termos MeSH primário: RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Células HeLa
Seres Humanos
Metagenômica
Metilação
Conformação de Ácido Nucleico
RNA Mensageiro/química
RNA Ribossômico/química
RNA Ribossômico/genética
Ribose/química
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (RNA, Ribosomal); 681HV46001 (Ribose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1038/nmeth.4294


  7 / 2907 MEDLINE  
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[PMID]:28495677
[Au] Autor:Zhu Y; Pirnie SP; Carmichael GG
[Ad] Endereço:Department of Genetics and Genome Sciences, UConn Health, Farmington, Connecticut 06030, USA.
[Ti] Título:High-throughput and site-specific identification of 2'- -methylation sites using ribose oxidation sequencing (RibOxi-seq).
[So] Source:RNA;23(8):1303-1314, 2017 Aug.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ribose methylation (2'- -methylation, 2'- Me) occurs at high frequencies in rRNAs and other small RNAs and is carried out using a shared mechanism across eukaryotes and archaea. As RNA modifications are important for ribosome maturation, and alterations in these modifications are associated with cellular defects and diseases, it is important to characterize the landscape of 2'- -methylation. Here we report the development of a highly sensitive and accurate method for ribose methylation detection using next-generation sequencing. A key feature of this method is the generation of RNA fragments with random 3'-ends, followed by periodate oxidation of all molecules terminating in 2',3'-OH groups. This allows only RNAs harboring 2'-OMe groups at their 3'-ends to be sequenced. Although currently requiring microgram amounts of starting material, this method is robust for the analysis of rRNAs even at low sequencing depth.
[Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala/métodos
RNA Ribossômico/análise
Ribose/química
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Células HeLa
Seres Humanos
Metilação
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal); 681HV46001 (Ribose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1261/rna.061549.117


  8 / 2907 MEDLINE  
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[PMID]:28378934
[Au] Autor:Selvam C; Mutisya D; Prakash S; Ranganna K; Thilagavathi R
[Ad] Endereço:Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
[Ti] Título:Therapeutic potential of chemically modified siRNA: Recent trends.
[So] Source:Chem Biol Drug Des;90(5):665-678, 2017 Nov.
[Is] ISSN:1747-0285
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Small interfering RNAs (siRNAs) are one of the valuable tools to investigate the functions of genes and are also used for gene silencing. It has a wide scope in drug discovery through in vivo target validation. siRNA therapeutics are not optimal drug-like molecules due to poor bioavailability and immunogenic and off-target effects. To overcome the challenges associated with siRNA therapeutics, identification of appropriate chemical modifications that improves the stability, specificity and potency of siRNA is essential. This review focuses on the various chemical modifications and their implications in siRNA therapy.
[Mh] Termos MeSH primário: RNA Interferente Pequeno/química
RNA Interferente Pequeno/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Imunomodulação
Simulação de Acoplamento Molecular
Fosfatos/química
Interferência de RNA
RNA Interferente Pequeno/imunologia
RNA Interferente Pequeno/metabolismo
Terapêutica com RNAi
Ribose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Phosphates); 0 (RNA, Small Interfering); 681HV46001 (Ribose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1111/cbdd.12993


  9 / 2907 MEDLINE  
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[PMID]:28302398
[Au] Autor:Chbib C
[Ad] Endereço:Department of Pharmaceutical Sciences, Larkin College of Pharmacy, 18301 North Miami Ave, Miami, FL 33169, United States. Electronic address: cchbib@ularkin.org.
[Ti] Título:Synthesis of isomeric analogues of S-ribosylhomocysteine analogues with homocysteine unit attached to C2 of ribose.
[So] Source:Bioorg Med Chem Lett;27(8):1681-1685, 2017 04 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:LuxS (S-ribosylhomocysteinase; EC 4.4.1.21) is an enzyme that catalyzes the cleavage of the thioether linkage in the catalytic pathway of S-ribosylhomocysteine (SRH) which produces homocysteine and 4,5-dihydroxy-2,3-pentanedione (DPD). DPD is the precursor of the signaling molecules known as autoinducer 2 (AI-2) responsible for the bacterial quorum sensing (QS) identified as cell to cell communication. Inhibitors of LuxS should be able to interfere with its catalytic pathway thus preventing the formation of the autoinducer molecules. In this work, the synthesis of 2-deoxy-2-bromo-SRH analogues was attempted by the coupling of the corresponding 2-bromo-2-deoxypentafuranosyl sugars with the homocysteinate anion. The displacement of the bromide from C2 rather than the expected substitution of the mesylate group from C5 was observed leading to a novel isomeric analogue of SRH in which Hcy moiety is attached to a ribose ring via C2-sulfur bond.
[Mh] Termos MeSH primário: Bacillus subtilis/enzimologia
Proteínas de Bactérias/antagonistas & inibidores
Liases de Carbono-Enxofre/antagonistas & inibidores
Inibidores Enzimáticos/química
Homocisteína/análogos & derivados
Ribose/análogos & derivados
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Liases de Carbono-Enxofre/metabolismo
Cristalografia por Raios X
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/farmacologia
Homocisteína/síntese química
Homocisteína/farmacologia
Isomerismo
Modelos Moleculares
Ribose/síntese química
Ribose/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Enzyme Inhibitors); 0LVT1QZ0BA (Homocysteine); 681HV46001 (Ribose); EC 4.4.- (Carbon-Sulfur Lyases); EC 4.4.1.21 (LuxS protein, Bacteria)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE


  10 / 2907 MEDLINE  
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[PMID]:28168603
[Au] Autor:Wang C; Liu Y; Zhang TT; Lu CG; Liu Y; Zhang DP; Liu WC
[Ad] Endereço:College of Food Science and Technology, Shi He Zi University, Xinjiang, 832003, China.
[Ti] Título:Metschnikowia persici sp. nov., A Novel Protease-Producing Yeast Species from China.
[So] Source:Curr Microbiol;74(3):365-370, 2017 Mar.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced protease and were presented as single separate group in the genus Metschnikowia by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of the three strains to all sequences of other yeasts accessed into the GenBank/EMBL/DDBJ and other database was very low (≦93%). The phylogenetic tree based on the D1/D2 region of 26S rRNA gene sequences revealed that three strains are most closely related to Metschnikowia koreensis KCTC 7828T (AF257272.1) (sequence similarity: 93.0%) and Metschnikowia reukaufii CBS9709 (AJ716113.1) (sequence similarity: 93.0%). However, the strains are distinguished from M. koreensis by its non-assimilation of galactose, ribitol, and D-xylose, and by its growth at 37 °C or in vitamin-free medium, and are notably different from M. reukaufii by its non-assimilation of galactose, D-xylose, D-arabinose, and D-ribose, and by its growth at 35 °C or in vitamin-free medium. The strain FHL-B formed asci in V8 juice sporulation medium for 3 weeks. Therefore, the name Metschnikowia persici is proposed for the novel species, with FHL-B (= CBS12815 = CFCC 3578T) as the type strain.
[Mh] Termos MeSH primário: Endopeptidases/metabolismo
Metschnikowia/enzimologia
Metschnikowia/metabolismo
Prunus persica/microbiologia
[Mh] Termos MeSH secundário: Arabinose/metabolismo
China
DNA Fúngico/genética
Endopeptidases/genética
Galactose/metabolismo
Metschnikowia/genética
RNA Ribossômico/genética
Ribitol/metabolismo
Ribose/metabolismo
Xilose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 26S); 488-81-3 (Ribitol); 681HV46001 (Ribose); A1TA934AKO (Xylose); B40ROO395Z (Arabinose); EC 3.4.- (Endopeptidases); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-017-1194-1



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