Base de dados : MEDLINE
Pesquisa : D10.212 [Categoria DeCS]
Referências encontradas : 5276 [refinar]
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[PMID]:29363926
[Au] Autor:Kodentsova VM; Kochetkova AA; Risnik DV; Sarkisyan VA; Bessonov VV
[Ti] Título:[The effect of microwaves on the fat component and preserve vitamins in foods].
[So] Source:Vopr Pitan;84(5):16-30, 2015.
[Is] ISSN:0042-8833
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Mh] Termos MeSH primário: Culinária/métodos
Gorduras na Dieta
Gorduras/química
Micro-Ondas
Valor Nutritivo
Vitamina E/química
[Mh] Termos MeSH secundário: Seres Humanos
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Fats); 1406-18-4 (Vitamin E)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  2 / 5276 MEDLINE  
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[PMID]:29244836
[Au] Autor:Schubert MM; Palumbo E; Seay RF; Spain KK; Clarke HE
[Ad] Endereço:Department of Kinesiology, California State University-San Marcos, San Marcos, CA, United States of America.
[Ti] Título:Energy compensation after sprint- and high-intensity interval training.
[So] Source:PLoS One;12(12):e0189590, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Many individuals lose less weight than expected in response to exercise interventions when considering the increased energy expenditure of exercise (ExEE). This is due to energy compensation in response to ExEE, which may include increases in energy intake (EI) and decreases in non-exercise physical activity (NEPA). We examined the degree of energy compensation in healthy young men and women in response to interval training. METHODS: Data were examined from a prior study in which 24 participants (mean age, BMI, & VO2max = 28 yrs, 27.7 kg•m-2, and 32 mL∙kg-1∙min-1) completed either 4 weeks of sprint-interval training or high-intensity interval training. Energy compensation was calculated from changes in body composition (air displacement plethysmography) and exercise energy expenditure was calculated from mean heart rate based on the heart rate-VO2 relationship. Differences between high (≥ 100%) and low (< 100%) levels of energy compensation were assessed. Linear regressions were utilized to determine associations between energy compensation and ΔVO2max, ΔEI, ΔNEPA, and Δresting metabolic rate. RESULTS: Very large individual differences in energy compensation were noted. In comparison to individuals with low levels of compensation, individuals with high levels of energy compensation gained fat mass, lost fat-free mass, and had lower change scores for VO2max and NEPA. Linear regression results indicated that lower levels of energy compensation were associated with increases in ΔVO2max (p < 0.001) and ΔNEPA (p < 0.001). CONCLUSIONS: Considerable variation exists in response to short-term, low dose interval training. In agreement with prior work, increases in ΔVO2max and ΔNEPA were associated with lower energy compensation. Future studies should focus on identifying if a dose-response relationship for energy compensation exists in response to interval training, and what underlying mechanisms and participant traits contribute to the large variation between individuals.
[Mh] Termos MeSH primário: Peso Corporal/fisiologia
Metabolismo Energético
Exercício/fisiologia
Perda de Peso/fisiologia
[Mh] Termos MeSH secundário: Adulto
Gorduras/metabolismo
Feminino
Treinamento Intervalado de Alta Intensidade
Seres Humanos
Masculino
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fats)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189590


  3 / 5276 MEDLINE  
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[PMID]:28543171
[Au] Autor:Yuwang P; Sulaeva I; Hell J; Henniges U; Böhmdorfer S; Rosenau T; Chitsomboon B; Tongta S
[Ad] Endereço:School of Food Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
[Ti] Título:Phenolic compounds and antioxidant properties of arabinoxylan hydrolysates from defatted rice bran.
[So] Source:J Sci Food Agric;98(1):140-146, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The water unextractable arabinoxylans (WUAX) contain beneficial phenolic compounds that can be used for food rather than for animal feed. The antioxidant activities of defatted rice bran obtained by xylanase-aided extraction is reported herein. The chemical and molecular characteristics of extracted fractions were investigated. RESULTS: The WUAX hydrolysate precipitated by 0-60% ethanol (F60), 60-90% ethanol (F6090), and more than 90% ethanol (F90) had decreased molar masses with increasing ethanol concentration. The fractions of interest, F60 and F6090, contained 75% arabinoxylans with ferulic acid as the major bound phenolic acid, followed by p-coumaric acid. According to chemical-based antioxidant assays F60 and F6090 exhibited higher diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric iron reducing ability than F90 which contained minor contents of small sugars and free phenolic acids. In cell-based antioxidant assays, using the fluorescent 2',7'-dichlorofluorescein diacetate probe, all three fractions were potent intracellular scavengers. CONCLUSION: The high molar mass of WUAX hydrolysates with high amount of bound phenolics contributes to the chemical-based antioxidant activity. All fractions of WUAX hydrolysates showed high potent intracellular scavenging activity regardless of molar mass, content and the component of bound phenolics. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Antioxidantes/química
Oryza/química
Fenóis/química
Extratos Vegetais/química
Sementes/química
[Mh] Termos MeSH secundário: Gorduras/análise
Oryza/enzimologia
Xilanos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fats); 0 (Phenols); 0 (Plant Extracts); 0 (Xylans); 9040-27-1 (arabinoxylan)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8448


  4 / 5276 MEDLINE  
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[PMID]:29080839
[Au] Autor:Li Y; Kang H; Chu Y; Jin Y; Zhang L; Yang R; Zhang Z; Zhao S; Zhou L
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, PR China.
[Ti] Título:Cidec differentially regulates lipid deposition and secretion through two tissue-specific isoforms.
[So] Source:Gene;641:265-271, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lipid metabolism has important roles in animal growth, development, and reproduction. As a regulator of lipid metabolism, CIDEc promotes unilocular development of lipid droplets and stimulates intracellular lipid deposition, and has two isoforms, CIDEc-l and CIDEc-s. CIDEc-l has ten more N-terminal amino acids than CIDEc-s. However, the functions of two isoforms are largely unknown. In this study, the expression profiles of two isoforms in Bama pigs differed, with cidec-l dominant in the liver and small intestine, and cidec-s dominant in muscle and adipose tissue. Fasting and consuming a high-fat diet resulted in changes in the expression of the two isoforms that were closely related to changes in blood and muscle triglyceride (TG) concentrations. Comparison of gene expression and TG concentration suggested that CIDEc-l accelerated lipid secretion and that CIDEc-s promoted lipid deposition, implying that the two isoforms had different functions. Study In vitro confirmed that CIDEc-s stimulated lipid deposition in C2C12 muscle cells and CIDEc-l promoted lipid secretion in HepG2 liver cells. The results showed that two tissue-specific CIDEc isoforms had different roles in lipid deposition and secretion. They may be potential targets for regulation of fat content.
[Mh] Termos MeSH primário: Metabolismo dos Lipídeos/fisiologia
Isoformas de Proteínas/metabolismo
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Tecido Adiposo/metabolismo
Tecido Adiposo/fisiologia
Animais
Linhagem Celular Tumoral
Dieta Hiperlipídica
Jejum/metabolismo
Jejum/fisiologia
Gorduras/metabolismo
Células Hep G2
Seres Humanos
Intestino Delgado/metabolismo
Intestino Delgado/fisiologia
Metabolismo dos Lipídeos/genética
Lipídeos/fisiologia
Fígado/metabolismo
Fígado/fisiologia
Músculos/metabolismo
Músculos/fisiologia
Isoformas de Proteínas/genética
Proteínas/genética
Sus scrofa
Transcriptoma/genética
Transcriptoma/fisiologia
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CIDEC protein, human); 0 (Fats); 0 (Lipids); 0 (Protein Isoforms); 0 (Proteins); 0 (Triglycerides)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171030
[St] Status:MEDLINE


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[PMID]:29066304
[Au] Autor:Bahnamiri HZ; Zali A; Ganjkhanlou M; Sadeghi M; Shahrbabak HM
[Ad] Endereço:Department of Animal Science, University of Tehran, P.O. Box # 3158711167-4111, Karaj, Iran. Electronic address: h.zakariapour@ut.ac.ir.
[Ti] Título:Regulation of lipid metabolism in adipose depots of fat-tailed and thin-tailed lambs during negative and positive energy balances.
[So] Source:Gene;641:203-211, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study aimed to evaluate the effects of negative and positive energy balances on gene expression of regulators and enzymes controlling lipogenesis and lipolysis in muscle and adipose depots of fat-tailed and thin-tailed lambs. Lambs were slaughtered during neutral, negative and positive energy balances for sample collection. Real time q-PCR was conducted to measure the gene expression. Expression of PPARγ was increased in response to positive energy balance regardless of genotype and type of tissue (P<0.04). Expression of SREBF1 was reduced in response to negative and positive energy balances in fat-tailed lambs, whereas in thin-tailed lambs, downregulated SREBF1 was restored during positive energy balance (P<0.01). Enhancement in FABP4 expression in response to negative and positive energy balances was respectively higher in thin-tailed and fat-tailed lambs affected by interaction of genotype and energy balance (P<0.11). In thin-tailed lambs, the enhanced FABP4 expression in response to negative energy balance was considerably higher in mesenteric adipose depot, whereas in fat-tailed lambs, positive energy balance induced enhancement in FABP4 expression was considerably higher in fat-tail adipose depot. The results demonstrate that transcription regulation of lipogenesis and lipolysis during negative and positive energy balances occurs differently in fat-tailed and thin-tailed lambs. Thin-tailed and fat-tailed lambs are respectively more responsive to negative and positive energy balances and mesenteric and fat-tail adipose depots respectively in thin-tailed and fat-tailed lambs are the main adipose depots responsible for higher responsiveness of thin-tailed and fat-tailed lambs to negative and positive energy balances.
[Mh] Termos MeSH primário: Tecido Adiposo/metabolismo
Metabolismo Energético/genética
Expressão Gênica/genética
Metabolismo dos Lipídeos/genética
[Mh] Termos MeSH secundário: Animais
Regulação para Baixo/genética
Gorduras/metabolismo
Proteínas de Ligação a Ácido Graxo/genética
Lipogênese/genética
Lipólise/genética
Masculino
Ovinos
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fats); 0 (Fatty Acid-Binding Proteins); 0 (Sterol Regulatory Element Binding Protein 1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171026
[St] Status:MEDLINE


  6 / 5276 MEDLINE  
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[PMID]:28966044
[Au] Autor:Yan Y; Wang H; Hu M; Jiang L; Wang Y; Liu P; Liang X; Liu J; Li C; Lindström-Battle A; Lam SM; Shui G; Deng WM; Jiao R
[Ad] Endereço:Sino-French Hoffmann Institute, School of Basic Sciences, Guangzhou Medical University, Dongfengxi Road 195, Guangzhou 510182, China; State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, the Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China.
[Ti] Título:HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.
[So] Source:Dev Cell;43(1):99-111.e5, 2017 Oct 09.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Age-dependent ectopic fat accumulation (EFA) in animals contributes to the progression of tissue aging and diseases such as obesity, diabetes, and cancer. However, the primary causes of age-dependent EFA remain largely elusive. Here, we characterize the occurrence of age-dependent EFA in Drosophila and identify HDAC6, a cytosolic histone deacetylase, as a suppressor of EFA. Loss of HDAC6 leads to significant age-dependent EFA, lipid composition imbalance, and reduced animal longevity on a high-fat diet. The EFA and longevity phenotypes are ameliorated by a reduction of the lipid-droplet-resident protein PLIN2. We show that HDAC6 is associated physically with the chaperone protein dHsc4/Hsc70 to maintain the proteostasis of PLIN2. These findings indicate that proteostasis collapse serves as an intrinsic cue to cause age-dependent EFA. Our study suggests that manipulation of proteostasis could be an alternative approach to the treatment of age-related metabolic diseases such as obesity and diabetes.
[Mh] Termos MeSH primário: Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Gorduras/metabolismo
Histona Desacetilases/metabolismo
Gotículas Lipídicas/metabolismo
Perilipina-2/metabolismo
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Autofagia/fisiologia
Citosol/metabolismo
Dieta Hiperlipídica
Drosophila melanogaster/genética
Desacetilase 6 de Histona
Longevidade/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Fats); 0 (Perilipin-2); EC 3.5.1.98 (HDAC6 protein, Drosophila); EC 3.5.1.98 (Histone Deacetylase 6); EC 3.5.1.98 (Histone Deacetylases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


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[PMID]:28923880
[Au] Autor:Umpleby AM; Shojaee-Moradie F; Fielding B; Li X; Marino A; Alsini N; Isherwood C; Jackson N; Ahmad A; Stolinski M; Lovegrove JA; Johnsen S; Jeewaka R Mendis AS; Wright J; Wilinska ME; Hovorka R; Bell JD; Thomas EL; Frost GS; Griffin BA
[Ad] Endereço:Faculty of Health and Medical Sciences, University of Surrey, Guildford, U.K.
[Ti] Título:Impact of liver fat on the differential partitioning of hepatic triacylglycerol into VLDL subclasses on high and low sugar diets.
[So] Source:Clin Sci (Lond);131(21):2561-2573, 2017 Nov 01.
[Is] ISSN:1470-8736
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dietary sugars are linked to the development of non-alcoholic fatty liver disease (NAFLD) and dyslipidaemia, but it is unknown if NAFLD itself influences the effects of sugars on plasma lipoproteins. To study this further, men with NAFLD ( = 11) and low liver fat 'controls' ( = 14) were fed two iso-energetic diets, high or low in sugars (26% or 6% total energy) for 12 weeks, in a randomised, cross-over design. Fasting plasma lipid and lipoprotein kinetics were measured after each diet by stable isotope trace-labelling.There were significant differences in the production and catabolic rates of VLDL subclasses between men with NAFLD and controls, in response to the high and low sugar diets. Men with NAFLD had higher plasma concentrations of VLDL -triacylglycerol (TAG) after the high ( <0.02) and low sugar ( <0.0002) diets, a lower VLDL -TAG fractional catabolic rate after the high sugar diet ( <0.01), and a higher VLDL -TAG production rate after the low sugar diet ( <0.01), relative to controls. An effect of the high sugar diet, was to channel hepatic TAG into a higher production of VLDL -TAG ( <0.02) in the controls, but in contrast, a higher production of VLDL -TAG ( <0.05) in NAFLD. These dietary effects on VLDL subclass kinetics could be explained, in part, by differences in the contribution of fatty acids from intra-hepatic stores, and de novo lipogenesis. The present study provides new evidence that liver fat accumulation leads to a differential partitioning of hepatic TAG into large and small VLDL subclasses, in response to high and low intakes of sugars.
[Mh] Termos MeSH primário: Carboidratos da Dieta/administração & dosagem
Gorduras/metabolismo
Lipoproteínas VLDL/metabolismo
Fígado/metabolismo
Hepatopatia Gordurosa não Alcoólica/metabolismo
Triglicerídeos/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Estudos Cross-Over
Carboidratos da Dieta/farmacologia
Ensaio de Imunoadsorção Enzimática
Jejum/sangue
Seres Humanos
Lipídeos/sangue
Lipoproteínas VLDL/sangue
Fígado/efeitos dos fármacos
Imagem por Ressonância Magnética
Masculino
Meia-Idade
Hepatopatia Gordurosa não Alcoólica/sangue
Avaliação de Resultados (Cuidados de Saúde)
Fatores de Tempo
Triglicerídeos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Dietary Carbohydrates); 0 (Fats); 0 (Lipids); 0 (Lipoproteins, VLDL); 0 (Triglycerides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1042/CS20171208


  8 / 5276 MEDLINE  
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[PMID]:28777952
[Au] Autor:Del Mundo DMN; Sutheerawattananonda M
[Ad] Endereço:School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand; Department of Food Science and Chemistry, College of Science and Mathematics, University of the Philippines Mindanao, Barangay Mintal, Davao City 8022, Philippines.
[Ti] Título:Influence of fat and oil type on the yield, physico-chemical properties, and microstructure of fat, oil, and grease (FOG) deposits.
[So] Source:Water Res;124:308-319, 2017 Nov 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fat, oil, and grease (FOG) deposit, in the form of calcium soap, was found to cause sanitary sewer overflows due to its adhesion on pipe walls. To address this problem, laboratory-prepared calcium soaps have been used to investigate the formation mechanisms of FOG deposits. However, the fats and oils previously utilized were limited and some soap characteristics were not examined. This research attempted to probe through the properties of calcium soaps prepared from calcium chloride and the fats and oils of chicken, pork, palm olein, soybean, olive, and coconut to further understand FOG formation and stability. Results revealed that FOG deposits may occur as smooth, paste-like material or coarse, semi-solid substance depending on their exposure to excess fat/oil and calcium. The smooth soaps with more excess fat/oil demonstrated high apparent viscosity and consistency index, while the coarse soaps with large levels of calcium signified higher melting endset. Moreover, a soap microstructure showing evident networks and lesser void area displayed higher heat and rheological stability, respectively. Overall, fats and oils with higher oleic to palmitic acid ratio such as palm olein oil, olive oil, chicken fat, and pork fat produced soaps with greater yield and degree of saponification. Hence, establishments and authorities should be alert in managing and monitoring these wastes. On the other hand, soybean oil high in linoleic acid and coconut oil high in lauric acid do not pose an immediate threat to the sewer system since they only produced soaps in small quantity. However, their soaps showed high melting endset which could pose a serious effect when accumulated at large amount. On the whole, the fatty acid profile of fats and oils, the presence of excess fat/oil, and calcium content mainly dictate the appearance, melting, rheology, and microstructure of calcium soaps. Their distinct properties can be used as criteria in predicting the condition and stability of FOG deposits.
[Mh] Termos MeSH primário: Gorduras
Óleos Vegetais
Carne Vermelha
Esgotos
[Mh] Termos MeSH secundário: Animais
Cálcio
Ácidos Graxos
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fats); 0 (Fatty Acids); 0 (Plant Oils); 0 (Sewage); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  9 / 5276 MEDLINE  
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[PMID]:28764026
[Au] Autor:Lorenzetti AS; Domini CE; Lista AG
[Ad] Endereço:INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Av. Alem 1253, 8000 Bahía Blanca, Argentina.
[Ti] Título:A simple and new reverse liquid-liquid microextraction for the automated spectrometric determination of doxycycline in chicken fat.
[So] Source:Food Chem;237:506-510, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work presents a new, simple and inexpensive reverse liquid-liquid microextraction of doxycycline (DOC) from chicken fat. In this just 13min extraction methodology, acidulated water, as extraction solvent (400µL), was used. A monochannel flow injection system was designed for the spectrometric determination of the analyte (ÊŽ=344nm). The extracted solution containing DOC was loaded into the injection valve of the continuous flow manifold. A lineal range between 100 and 700µgDOCkg sample was obtained. The LOD and LOQ were 33µgkg and 100µgkg respectively. The relative standard deviation was 4.87% and the sample throughput for the entire process was 4.5h . As recovery values when the method was applied to real samples showed variability, the expanded uncertainties were calculated. Their values indicated that the new method is independent of the concentration of the analyte and the origin of the sample.
[Mh] Termos MeSH primário: Galinhas
Doxiciclina/análise
Gorduras/química
[Mh] Termos MeSH secundário: Animais
Microextração em Fase Líquida
Solventes
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fats); 0 (Solvents); 059QF0KO0R (Water); N12000U13O (Doxycycline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


  10 / 5276 MEDLINE  
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[PMID]:28694735
[Au] Autor:Cadamuro J; Wiedemann H; Felder TK; Mrazek C; Kipman U; Hannes O; Haschke-Becher E
[Ad] Endereço:Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria.
[Ti] Título:What´s floating on my plasma?
[So] Source:Biochem Med (Zagreb);27(2):430-433, 2017 Jun 15.
[Is] ISSN:1330-0962
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:We report on a preanalytical issue we encountered during routine clinical chemistry analyses, potentially leading to deviated analysis results and believe that it might help other laboratories to overcome similar problems. In a heparin-gel tube we measured an implausible glucose value of 0.06 mmol/L. Re-measurement of the same sample resulted in a glucose value of 5.4 mmol/L. After excluding an analytical error, we inspected the sample closer and found a white material as well as fatty droplets floating on the surface of the plasma tube. Evaluation of these structures revealed that the white particulate matter (WPM) consisted of fibrinogen, platelets and leukocytes and the fatty droplets most probably originated from the separator gel. We concluded that these structures formed a temporary clot in the instruments pipetting needle thereby altering the sampling volume and subsequently the measured glucose value. The formation of WPM might be attributable to high speed centrifugation, high cholesterol levels, the gel formulation or a combination of several issues such as temperature, heparin concentration, pH and patient-specific factors. The gel droplets were most probably caused by an aberrant gel formulation in combination with an improper storage of the empty tubes on the wards prior to phlebotomy. After adding an additional instrument cleansing cycle and changing to another batch of heparin tubes the problems could be significantly reduced.
[Mh] Termos MeSH primário: Análise Química do Sangue/normas
Glicemia/análise
Coleta de Amostras Sanguíneas/normas
Heparina/química
[Mh] Termos MeSH secundário: Análise Química do Sangue/métodos
Plaquetas/química
Coleta de Amostras Sanguíneas/instrumentação
Coleta de Amostras Sanguíneas/métodos
Colesterol/química
Gorduras/química
Fibrinogênio/química
Géis/química
Seres Humanos
Concentração de Íons de Hidrogênio
Leucócitos/química
Material Particulado/química
Reprodutibilidade dos Testes
Trombose
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Fats); 0 (Gels); 0 (Particulate Matter); 9001-32-5 (Fibrinogen); 9005-49-6 (Heparin); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.11613/BM.2017.047



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