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  1 / 1391 MEDLINE  
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[PMID]:29367484
[Au] Autor:Yamamoto Y; Yoshida H; Nagai T; Hara S
[Ad] Endereço:Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima.
[Ti] Título:Preparation of Chiral Triacylglycerols, sn-POO and sn-OOP, via Lipase-mediated Acidolysis Reaction.
[So] Source:J Oleo Sci;67(2):207-214, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:It is well known that lipases are useful tools for preparing various structured triacylglycerols (TAGs). However, the lipase-mediated preparation of chiral TAGs has never been reported. This study aimed to prepare chiral TAGs (viz., 1-palmitoyl-2,3-dioleoyl-sn-glycerol (sn-POO) or 1,2-dioleoyl-3-palmitoyl-sn-glycerol (sn-OOP)) via lipase mediated acidolysis, using triolein (TO) and palmitic acid (P) as substrates. Three commercially available lipases (viz., Lipozyme RM-IM , Lipozyme TL-IM , and Lipase OF ) were used. Lipozyme RM-IM resulted in an increase 1P-2O (sn-POO + sn-OOP + 1,3-dioleoyl-2-palmitoyl-sn-glycerol) content with reaction time, which plateaued at 2~24 h (max. yield 47.1% at 4 h). The highest sn-POO/sn-OOP ratio of ca. 9 was obtained at 0.25 h, and the rate got close to 1 with reaction time (sn-POO/sn-OOP = 1.3 at 24 h). Lipozyme TL-IM resulted in a lower 1P-2O synthesis rate than Lipozyme RM-IM , where its highest sn-POO/sn-OOP ratio of ca. 2 was obtained at 0.25 h and did not vary much further with reaction time. In the case of Lipase OF , its reaction rate for 1P-2O synthesis was lower than that of the other two lipases, and the highest sn-POO/sn-OOP ratio of ca. 1.4 was obtained at 0.5 h, reaching closer to 1 with a longer reaction time. Reaction solvents (viz., hexane, acetone, and benzene) also affected the 1P-2O preparation, where the highest 1P-2O content was obtained with the solvent-free system. Furthermore, the solvent-free system showed a higher reaction rate for 1P-2O synthesis than did the hexane system, with no effect on chiral specificity of the lipase for the TAG molecules. These results suggested that among three types of commercial lipase, Lipozyme RM-IM is the most useful for the preparation of chiral TAGs by acidolysis reaction.
[Mh] Termos MeSH primário: Lipase/química
Triglicerídeos/síntese química
[Mh] Termos MeSH secundário: Ácido Palmítico/química
Solventes
Estereoisomerismo
Fatores de Tempo
Triglicerídeos/química
Trioleína/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Solvents); 0 (Triglycerides); 122-32-7 (Triolein); 2V16EO95H1 (Palmitic Acid); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17149


  2 / 1391 MEDLINE  
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[PMID]:28744120
[Au] Autor:Chakraborty S; Kar N; Kumari L; De A; Bera T
[Ad] Endereço:Laboratory of Nanomedicine, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
[Ti] Título:Inhibitory effect of a new orally active cedrol-loaded nanostructured lipid carrier on compound 48/80-induced mast cell degranulation and anaphylactic shock in mice.
[So] Source:Int J Nanomedicine;12:4849-4868, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Type I hypersensitivity is an allergic reaction characterized by the overactivity of the immune system provoked by normally harmless substances. Glucocorticoids, anti-histamines, or mast cell stabilizers are the choices of treatment for type I hypersensitivity. Even though these drugs have the anti-allergic effect, they can have several side effects in prolong use. Cedrol is the main bioactive compound of with anti-tumor, anti-oxidative, and platelet-activating factor inhibiting properties. METHODS: In this study, the preparation and anti-anaphylactic effect of cedrol-loaded nanostructured lipid carriers (NLCs) were evaluated. NLCs were prepared using Compritol 888 ATO and triolein as lipid phase and vitamin E d-α-tocopherylpolyethyleneglycol 1000 succinate, soya lecithin, and sodium deoxycholate as nanoparticle stabilizers. RESULTS: The average diameter of cedrol-NLCs (CR-NLCs) was 71.2 nm (NLC-C ) and 91.93 nm (NLC-C ). The particle had negative zeta potential values of -31.9 mV (NLC-C ) and -44.5 mV (NLC-C ). Type I anaphylactoid reaction in the animal model is significantly reduced by cedrol and cedrol-NLC. This in vivo activity of cedrol resulted that cedrol suppressed compound 48/80-induced peritoneal mast cell degranulation and histamine release from mast cells. Furthermore, compound 48/80-evoked Ca uptake into mast cells was reduced in a dose-dependent manner by cedrol and cedrol-NLC. Studies confirmed that the inhibition of type I anaphylactoid response in vivo in mice and compound 48/80-induced mast cell activation in vitro are greatly enhanced by the loading of cedrol into the NLCs. The safety of cedrol and CR-NLC was evaluated as selectivity index (SI) with prednisolone and cromolyn sodium as positive control. SI of CR-NLC-C was found to be 11.5-fold greater than both prednisolone and cromolyn sodium. CONCLUSION: Administration of CR-NLC 24 hours before the onset of anaphylaxis can prevent an anaphylactoid reaction. NLCs could be a promising vehicle for the oral delivery of cedrol to protect anaphylactic reactions.
[Mh] Termos MeSH primário: Anafilaxia/tratamento farmacológico
Portadores de Fármacos/química
Mastócitos/efeitos dos fármacos
Nanoestruturas/administração & dosagem
Terpenos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Degranulação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Portadores de Fármacos/administração & dosagem
Ácidos Graxos
Feminino
Liberação de Histamina/efeitos dos fármacos
Lipídeos/administração & dosagem
Lipídeos/química
Masculino
Mastócitos/fisiologia
Camundongos Endogâmicos BALB C
Nanopartículas/química
Nanoestruturas/química
Terpenos/farmacologia
Trioleína/química
Vitamina E/química
p-Metoxi-N-metilfenetilamina/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Fatty Acids); 0 (Lipids); 0 (Terpenes); 122-32-7 (Triolein); 1406-18-4 (Vitamin E); 18641-57-1 (glyceryl behenate); 4091-50-3 (p-Methoxy-N-methylphenethylamine); 63ZM9703BO (cedrol); O03S90U1F2 (tocophersolan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S132114


  3 / 1391 MEDLINE  
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[PMID]:29303272
[Au] Autor:Chai XH; Meng Z; Cao PR; Jiang J; Liu YF
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University , 1800 Lihu Road, Wuxi 214122, Jiangsu, P
[Ti] Título:Comparative Analysis of Small-Molecule Diffusivity in Different Fat Crystal Network.
[So] Source:J Agric Food Chem;66(4):1015-1022, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oil migration and fat recrystallization in fat-structured food materials can result in significant deterioration in food quality. Consequently, it is important to monitor and quantify the diffusivities of the migrants in fat crystal network. The diffusion coefficients of Nile red dye in liquid oils through fully hydrogenated palm kernel oil (FHPKO)/triolein (OOO) and fully hydrogenated soybean oil (FHSO)/triolein (OOO) systems were evaluated by the fluorescence recovery after photobleaching (FRAP) method. The effective diffusion coefficients (D ) and mobile fraction (M ) increased with the decrease of solid fat contents (SFC), with the changes of microstructure from more densely to slightly larger packed clusters for both FHPKO/OOO and FHSO/OOO systems. In addition, microstructural parameters of these systems were estimated by the image analysis. The results showed that the diffusion of dye and liquid oil was affected by the microstructure. The higher D was associated with lower fractal dimensions, larger crystal thickness, and larger average particle sizes. Finally, higher-permeability coefficients were calculated according to Darcy's Law, and it was significantly correlated to the D .
[Mh] Termos MeSH primário: Óleos Vegetais/química
[Mh] Termos MeSH secundário: Cristalização
Difusão
Recuperação de Fluorescência Após Fotodegradação
Corantes Fluorescentes
Microscopia Confocal
Oxazinas
Óleo de Palmeira/química
Óleo de Soja/química
Trioleína/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Oxazines); 0 (Plant Oils); 122-32-7 (Triolein); 5QUO05548Z (Palm Oil); 8001-22-7 (Soybean Oil); P476F1L81G (nile red)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04677


  4 / 1391 MEDLINE  
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[PMID]:28402884
[Au] Autor:Bacle A; Gautier R; Jackson CL; Fuchs PF; Vanni S
[Ad] Endereço:Institut Jacques Monod, UMR 7592, CNRS Université Paris-Diderot, Sorbonne Paris Cité, Paris, France.
[Ti] Título:Interdigitation between Triglycerides and Lipids Modulates Surface Properties of Lipid Droplets.
[So] Source:Biophys J;112(7):1417-1430, 2017 Apr 11.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intracellular lipid droplets (LDs) are the main cellular site of metabolic energy storage. Their structure is unique inside the cell, with a core of esterified fatty acids and sterols, mainly triglycerides and sterol esters, surrounded by a single monolayer of phospholipids. Numerous peripheral proteins, including several that were previously associated with intracellular compartments surrounded by a lipid bilayer, have been recently shown to target the surface of LDs, but how they are able to selectively target this organelle remains largely unknown. Here, we use atomistic and coarse-grained molecular dynamics simulations to investigate the molecular properties of the LD surface and to characterize how it differs from that of a lipid bilayer. Our data suggest that although several surface properties are remarkably similar between the two structures, key differences originate from the interdigitation between surface phospholipids and core neutral lipids that occurs in LDs. This property is extremely sensitive to membrane undulations, unlike in lipid bilayers, and it strongly affects both lipid-packing defects and the lateral pressure profile. We observed a marked change in overall surface properties for surface tensions >10 mN/m, indicative of a bimodal behavior. Our simulations provide a comprehensive molecular characterization of the unique surface properties of LDs and suggest how the molecular properties of the surface lipid monolayer can be modulated by the underlying neutral lipids.
[Mh] Termos MeSH primário: Gotículas Lipídicas/química
Lipídeos/química
Triglicerídeos/química
[Mh] Termos MeSH secundário: Conformação Molecular
Simulação de Dinâmica Molecular
Tamanho da Partícula
Fosfatidilcolinas/química
Fosfolipídeos/química
Pressão
Tensão Superficial
Trioleína/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (Phosphatidylcholines); 0 (Phospholipids); 0 (Triglycerides); 122-32-7 (Triolein); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


  5 / 1391 MEDLINE  
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[PMID]:28135401
[Au] Autor:Li H; Pan Y; Luo K; Luo T; Fan Y; Deng Z
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
[Ti] Título:Effects of Different Simple Triglycerides on Cell Fatty Acid Compositions, Proliferation-Related Protein, and Gene Expressions Induced by Oxidized-LDL in HUVSMCs.
[So] Source:J Food Sci;82(2):529-535, 2017 Feb.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The proliferating effects of 3 different simple triglycerides (tristearin, triolein, and trilinolein) on the human umbilical vein smooth muscle cells (HUVSMCs) induced by oxidized-LDL (ox-LDL) were investigated in this study. The protein and mRNA gene expressions of proliferating cell nuclear antigen (PCNA), smooth muscle-α-actin (SM-α-actin), and monocyte chemoattractant protein-1 (MCP-1) in HUVSMCs were measured by Western blotting and real-time quantitative polymerase chain reaction (PCR). It was shown that in tristearin (SSS) treated HUVSMCs, the saturated fatty acid content was increased, and the compositions of polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid were decreased significantly. On the other hand, triolein (OOO) and trilinolein (LLL) significantly increased the levels of some typical PUFA such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. Moreover, LLL and OOO could upregulate the protein and mRNA expressions of PCNA, MCP-1 as well as downregulate the expression of SM-α-actin. The results also showed that, SSS had significant promotion effects on the proliferation of HUVSMCs induced by ox-LDL. Although both LLL and OOO could inhibit the proliferation of HUVSMCs induced by ox-LDL, and might have certain inhibition of the atherosclerotic process.
[Mh] Termos MeSH primário: Lipoproteínas LDL/metabolismo
Miócitos de Músculo Liso/metabolismo
Antígeno Nuclear de Célula em Proliferação/metabolismo
Triglicerídeos/química
[Mh] Termos MeSH secundário: Actinas/metabolismo
Aterosclerose/metabolismo
Células Cultivadas
Quimiocina CCL2/metabolismo
Ácidos Graxos Insaturados/química
Expressão Gênica/efeitos dos fármacos
Regulação da Expressão Gênica
Seres Humanos
Músculo Liso Vascular/citologia
Trioleína/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (CCL2 protein, human); 0 (Chemokine CCL2); 0 (Fatty Acids, Unsaturated); 0 (Lipoproteins, LDL); 0 (Proliferating Cell Nuclear Antigen); 0 (Triglycerides); 0 (oxidized low density lipoprotein); 122-32-7 (Triolein); P6OCJ2551R (tristearin); V5LJ52OGS7 (trilinolein)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13621


  6 / 1391 MEDLINE  
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[PMID]:28118562
[Au] Autor:Dalmia A
[Ad] Endereço:Perkin Elmer, 710 Bridgeport Ave, Shelton, CT 06484.
[Ti] Título:Rapid Measurement of Food Adulteration with Minimal Sample Preparation and No Chromatography Using Ambient Ionization Mass Spectrometry.
[So] Source:J AOAC Int;100(2):573-575, 2017 Mar 01.
[Is] ISSN:1060-3271
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A rapid method, with minimal sample preparation and no chromatography, was developed for analyzing food samples such as olive oil and pomegranate juice to measure adulteration with cheaper ingredients using the novel Direct Sample Analysis™ (DSA) ion source in conjunction with a time-of-flight (TOF)-MS. In less than 30 s, with minimal sample preparation and method development, adulteration of olive oil and pomegranate juice with cheaper seed oils and fruit juices, respectively, was measured with DSA/TOF-MS.
[Mh] Termos MeSH primário: Contaminação de Alimentos/análise
Sucos de Frutas e Vegetais/análise
Azeite de Oliva/análise
[Mh] Termos MeSH secundário: Malatos/análise
Espectrometria de Massas
Olea
Punicaceae
Óleo de Soja/análise
Tartaratos/análise
Triglicerídeos/análise
Trioleína/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1,2-dioleoyl-3-palmitoylglycerol); 0 (1,3-dilinolenoyl-2-palmitoylglycerol); 0 (Malates); 0 (Olive Oil); 0 (Tartrates); 0 (Triglycerides); 122-32-7 (Triolein); 8001-22-7 (Soybean Oil); 817L1N4CKP (malic acid); V5LJ52OGS7 (trilinolein); W4888I119H (tartaric acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.5740/jaoacint.16-0343


  7 / 1391 MEDLINE  
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[PMID]:28114790
[Au] Autor:Yamaguchi T; Murano T; Tatsuno I; Hiruta N; Suzuki T; Sawada S; Katagiri H; Shirai K; Schneider WJ; Bujo H
[Ad] Endereço:1 Center of Diabetes, Endocrinology and Metabolism, Toho University Sakura Medical Center, Sakura, Japan.
[Ti] Título:Severely impaired activity of lipoprotein lipase Arg243His is partially ameliorated by emulsifying phospholipids in in vitro triolein hydrolysis analysis.
[So] Source:Ann Clin Biochem;54(6):712-715, 2017 Nov.
[Is] ISSN:1758-1001
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsified with Triton X-100. Methods Lipolytic activities of plasma obtained by heparin injection from a homozygous patient with LPL Arg243His were analysed using triolein emulsified with phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), or Triton X-100 as substrates. Results The hydrolysing activities of the patient's plasma for triolein emulsified with PC, PE, PS, PI, LPC and Triton X-100 were 9.22 ± 1.06 µmol/ml/h/ngLPL, 2.94 ± 1.60 µmol/ml/h/ng LPL, 3.72 ± 1.63 µmol/ml/h/ng LPL, 3.40 ± 1.20 µmol/ml/h/ngLPL, 3.72 ± 1.96 µmol/ml/h/ngLPL and 7.80 ± 4.48 µmol/ml/h/ng LPL, respectively. Thus, the specific activities of the patient's LPL determined with triolein emulsified with PC were significantly higher than those with PE, PS, PI or LPC as emulsifiers. Relative to the activities of normal plasma measured with PC, PE, PS, PI and LPC as emulsifiers, the mutant's activities were 49.1 ± 5.2%, 44.1 ± 5.7%, 31.7 ± 12.6%, 19.2 ± 6.9% and 23.8 ± 11.3%, respectively. Using PC, PE, PS, PI and LPC as emulsifiers, the mutant's activities for triolein-lipolysis relative to normal were significantly increased in comparison to the relative activity measured with the classical emulsifier, Triton X-100 (12.9 ± 6.7%). Conclusions Impaired triolein hydrolysis by LPL Arg243His was partially ameliorated by triolein emulsification with phospholipids. The in vitro analysis of triolein hydrolysis using various phospholipid emulsifiers may be useful for the further understanding of impaired LPL function.
[Mh] Termos MeSH primário: Substituição de Aminoácidos
Emulsificantes/farmacologia
Lipase Lipoproteica/genética
Lipase Lipoproteica/metabolismo
Fosfolipídeos/farmacologia
Trioleína/metabolismo
[Mh] Termos MeSH secundário: Idoso
Feminino
Homozigoto
Seres Humanos
Hidrólise/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsifying Agents); 0 (Phospholipids); 122-32-7 (Triolein); EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1177/0004563217693258


  8 / 1391 MEDLINE  
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[PMID]:28107310
[Au] Autor:Fletcher AN; Molteni A; Ponnapureddy R; Patel C; Pluym M; Poisner AM
[Ad] Endereço:From the University of Kansas Medical Center, Department of Preventive Medicine and Public Health (A.N.F.); Department of Pathology (A.M., C.P.); Department of Internal Medicine (R.P., M.P.), University of Missouri; and Department of Pharmacology and Toxicology (A.M.P.), University of Kansas Medical Center, Kansas, MO.
[Ti] Título:The renin inhibitor aliskiren protects rat lungs from the histopathologic effects of fat embolism.
[So] Source:J Trauma Acute Care Surg;82(2):338-344, 2017 02.
[Is] ISSN:2163-0763
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fat embolism (FE) and the consequent FE syndrome occurring after trauma or surgery can lead to serious pulmonary injury, including ARDS and death. Current treatment of FE syndrome is limited to supportive therapy. We have shown in a rat model that the renin angiotensin system plays a significant role in the pathophysiology of FE because drugs interfering with the renin angiotensin system, captopril and losartan reduce the histopathologic pulmonary damage. The purpose of the current study was to determine if inhibition of renin by aliskiren, an FDA-approved drug for treating hypertension, would produce effective protection in the same model. METHODS: The FE model used intravenous injection of the neutral fat triolein in unanesthetized rats. Intraperitoneal injections of saline or aliskiren at either 50 or 100 mg/kg were performed 1 hour after FE induction via triolein. Rats were euthanized at 48 hours, and various histologic stains were used to examine the lungs. RESULTS: (1) Fibrosis: rats treated with triolein showed significant fibrotic changes with increased collagen and myofibroblast activation (p < 0.0001 for both trichrome and α-smooth muscle actin staining). Aliskiren blocked this inflammatory and profibrotic process to a level indistinguishable from the controls (p < 0.0001 for both trichrome and α-smooth muscle actin staining). (2) Fat: rats treated with triolein showed a statistically significant increase in fat (p = 0.0006). Subsequent aliskiren administration at both doses reduced the size, distribution, and amount of fat droplets (low dose, p = 0.0095; high dose, p = 0.0028). (3) Vessel patency: the low dose of aliskiren blocked the reduction of lumen patency observed after triolein administration (p = 0.0058). CONCLUSIONS: Aliskiren protected the lungs of rats from gross and histopathologic FE-induced pulmonary damage at 48 hours. Clinical implications include the use of aliskiren both prophylactically (before certain orthopedic procedures) and therapeutically (after severe trauma) to prevent the consequent severe pulmonary pathologic sequelae.
[Mh] Termos MeSH primário: Amidas/farmacologia
Embolia Gordurosa/prevenção & controle
Fumaratos/farmacologia
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Masculino
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Trioleína/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Fumarates); 122-32-7 (Triolein); 502FWN4Q32 (aliskiren)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1097/TA.0000000000001278


  9 / 1391 MEDLINE  
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[PMID]:27932255
[Au] Autor:Leirós GJ; Kusinsky AG; Balañá ME; Hagelin K
[Ad] Endereço:Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: gleiros@fundacioncassara.org.ar.
[Ti] Título:Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes.
[So] Source:J Dermatol Sci;85(2):124-130, 2017 Feb.
[Is] ISSN:1873-569X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cytokine production and oxidative stress generated by ultraviolet radiation B (UVB) skin exposure are main factors of skin photoaging. Interleukin-6 (IL-6) produced by irradiated keratinocytes is proposed to have a role in metalloproteinases (MMPs) expression activation in dermal fibroblasts. OBJECTIVES: We examined the effect of triolein treatment of UVB-irradiated keratinocytes on MMP1 (interstitial collagenase) expression response of dermal fibroblasts. We assayed UVB-irradiated keratinocytes soluble signals, mainly IL-6 and reactive oxygen species (ROS). METHODS: IL-6 expression and ROS generation were assayed in UVB-irradiated keratinocytes. MMP1 mRNA expression response was assayed in fibroblasts grown in keratinocytes conditioned medium. We evaluated the effect of treating keratinocytes with triolein on IL-6 expression and ROS generation in keratinocytes, and MMP1 expression in fibroblasts. RESULTS: The irradiation of epidermal cells with sublethal UVB doses increased IL-6 expression and ROS generation. Conditioned culture medium collected from keratinocytes was used to culture dermal fibroblasts. MMP1 mRNA expression increase was observed in fibroblasts cultured in medium collected from UVB-irradiated keratinocytes. Triolein treatment reduced the IL-6 expression and ROS generation in keratinocytes and this effect was reflected in downregulation of MMP1 expression in fibroblasts. CONCLUSIONS: Triolein reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes. It seems to exert an anti-inflammatory and anti-oxidative stress effect on irradiated keratinocytes that in turn reduces MMP1 expression in dermal fibroblasts. Collectively, these results indicate that triolein could act as a photoprotective agent.
[Mh] Termos MeSH primário: Queratinócitos/efeitos dos fármacos
Metaloproteinase 1 da Matriz/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Protetores Solares/farmacologia
Trioleína/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Linhagem Celular
Meios de Cultivo Condicionados
Derme/citologia
Derme/efeitos dos fármacos
Derme/enzimologia
Derme/efeitos da radiação
Regulação para Baixo
Fibroblastos/efeitos dos fármacos
Fibroblastos/enzimologia
Seres Humanos
Interleucina-6/metabolismo
Interleucina-6/secreção
Queratinócitos/enzimologia
Queratinócitos/efeitos da radiação
Estresse Oxidativo/efeitos dos fármacos
RNA Mensageiro/metabolismo
Envelhecimento da Pele/efeitos dos fármacos
Envelhecimento da Pele/efeitos da radiação
Raios Ultravioleta/efeitos adversos
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Culture Media, Conditioned); 0 (IL6 protein, human); 0 (Interleukin-6); 0 (RNA, Messenger); 0 (Reactive Oxygen Species); 0 (Sunscreening Agents); 122-32-7 (Triolein); EC 3.4.24.7 (MMP1 protein, human); EC 3.4.24.7 (Matrix Metalloproteinase 1)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE


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[PMID]:27547896
[Au] Autor:Basu C; Ahmed MA; Kartha RV; Brundage RC; Raymond GV; Cloyd JC; Carlin BP
[Ad] Endereço:a Division of Biostatistics , School of Public Health, University of Minnesota , Minneapolis , Minnesota , USA.
[Ti] Título:A hierarchical Bayesian approach for combining pharmacokinetic/pharmacodynamic modeling and Phase IIa trial design in orphan drugs: Treating adrenoleukodystrophy with Lorenzo's oil.
[So] Source:J Biopharm Stat;26(6):1025-1039, 2016.
[Is] ISSN:1520-5711
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:X-linked adrenoleukodystrophy (X-ALD) is a rare, progressive, and typically fatal neurodegenerative disease. Lorenzo's oil (LO) is one of the few X-ALD treatments available, but little has been done to establish its clinical efficacy or indications for its use. In this article, we analyze data on 116 male asymptomatic pediatric patients who were administered LO. We offer a hierarchical Bayesian statistical approach to understand LO pharmacokinetics (PK) and pharmacodynamics (PD) resulting from an accumulation of very long-chain fatty acids. We experiment with individual- and observational-level errors and various choices of prior distributions and deal with the limitation of having just one observation per administration of the drug, as opposed to the more usual multiple observations per administration. We link LO dose to the plasma erucic acid concentrations by PK modeling, and then link this concentration to a biomarker (C26, a very long-chain fatty acid) by PD modeling. Next, we design a Bayesian Phase IIa study to estimate precisely what improvements in the biomarker can arise from various LO doses while simultaneously modeling a binary toxicity endpoint. Our Bayesian adaptive algorithm emerges as reasonably robust and efficient while still retaining good classical (frequentist) operating characteristics. Future work looks toward using the results of this trial to design a Phase III study linking LO dose to actual improvements in health status, as measured by the appearance of brain lesions observed via magnetic resonance imaging.
[Mh] Termos MeSH primário: Adrenoleucodistrofia/tratamento farmacológico
Teorema de Bayes
Ensaios Clínicos Fase II como Assunto
Ácidos Erúcicos/farmacocinética
Projetos de Pesquisa
Trioleína/farmacocinética
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Combinação de Medicamentos
Ácidos Erúcicos/sangue
Ácidos Erúcicos/uso terapêutico
Seres Humanos
Masculino
Produção de Droga sem Interesse Comercial
Trioleína/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Erucic Acids); 0 (Lorenzo's oil); 075441GMF2 (erucic acid); 122-32-7 (Triolein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE



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