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[PMID]:24649980
[Au] Autor:van den Berk LC; Jansen BJ; Snowden S; Siebers-Vermeulen KG; Gilissen C; Kögler G; Figdor CG; Wheelock CE; Torensma R
[Ad] Endereço:1 Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre , Nijmegen, The Netherlands .
[Ti] Título:Cord blood mesenchymal stem cells suppress DC-T Cell proliferation via prostaglandin B2.
[So] Source:Stem Cells Dev;23(14):1582-93, 2014 Jul 15.
[Is] ISSN:1557-8534
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immune suppression is a very stable property of multipotent stromal cells also known as mesenchymal stem cells (MSCs). All cell lines tested showed robust immune suppression not affected by a long culture history. Several mechanisms were described to account for this capability. Since several of the described mechanisms were not causing the immune suppression, the expression pattern of cord-blood-derived MSCs by microarray experiments was determined. Dendritic cells cocultured with cord blood MSCs were compared with cord blood MSCs. Putative immune suppressive candidates were tested to explain this inhibition. We find that cord blood MSCs themselves are hardly immunogenic as tested with allogeneic T-cells. Dendritic cells cocultured with second-party T-cells evoked abundant proliferation that was inhibited by third-party cord blood MSCs. Optimal inhibition was seen with one cord blood MSC for every dendritic cell. Blocking human leukocyte antigen G only saw partial recovery of proliferation. Several cytokines, gangliosides, enzymes like arginase, NO synthase, and indole amine 2,3-dioxygenase as well as the induction of Treg were not involved in the inhibition. The inhibiting moiety was identified as prostaglandin B2 by lipid metabolite analysis of the culture supernatant and confirmed with purified prostaglandin B2.
[Mh] Termos MeSH primário: Proliferação Celular/genética
Imunossupressão
Células Mesenquimais Estromais/imunologia
Prostaglandinas B/metabolismo
[Mh] Termos MeSH secundário: Técnicas de Cocultura
Células Dendríticas/imunologia
Células Dendríticas/patologia
Sangue Fetal/imunologia
Antígenos HLA/imunologia
Antígenos HLA/metabolismo
Seres Humanos
Células Mesenquimais Estromais/patologia
Prostaglandinas B/imunologia
Linfócitos T/imunologia
Linfócitos T/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HLA Antigens); 0 (Prostaglandins B); BIU61O9T9T (prostaglandin B2)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140322
[St] Status:MEDLINE
[do] DOI:10.1089/scd.2013.0433


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[PMID]:20945130
[Au] Autor:Dang TH; Lee HJ; Yoo ES; Hong J; Choi JS; Jung JH
[Ad] Endereço:College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
[Ti] Título:The occurrence of 15-keto-prostaglandins in the red alga Gracilaria verrucosa.
[So] Source:Arch Pharm Res;33(9):1325-9, 2010 Sep.
[Is] ISSN:0253-6269
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:New 15-keto-prostaglandins (1-4) were isolated from the MeOH extract of the red alga, Gracilaria verrucosa. Their structures were determined to be prostaglandin B congeners (1-3) and a prostaglandin E congener (4) based on the NMR and MS data. Prostaglandins with a C-15 keto function are rare from natural sources. The presence of these metabolites in the alga is notable because 15-keto-prostaglandins (15-keto-PGs) are considered to be the metabolic products of regular prostaglandins in mammals. The occurrence of different prostaglandins in this alga might be due to the existence of different oxidative enzymes, as previously mentioned for oxygenated fatty acids of the red alga Gracilariopsis lemaneiformis. The antiinflammatory activity of these prostaglandins was examined by evaluating their inhibitory effects on nitric oxide production in lipopolysaccharide (LPS)-activated RAW264.7 murine macrophage cells. These prostaglandins showed weak activity on nitric oxide production.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/análise
Anti-Inflamatórios não Esteroides/química
Descoberta de Drogas
Gracilaria/química
Prostaglandinas/análise
Prostaglandinas/química
[Mh] Termos MeSH secundário: Alprostadil/análogos & derivados
Alprostadil/análise
Alprostadil/química
Alprostadil/isolamento & purificação
Alprostadil/farmacologia
Animais
Anti-Inflamatórios não Esteroides/isolamento & purificação
Anti-Inflamatórios não Esteroides/farmacologia
Linhagem Celular Transformada
Sobrevivência Celular/efeitos dos fármacos
Lipopolissacarídeos/toxicidade
Ativação de Macrófagos/efeitos dos fármacos
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Espectroscopia de Ressonância Magnética
Camundongos
Estrutura Molecular
Óxido Nítrico/metabolismo
Extratos Vegetais/química
Prostaglandinas/isolamento & purificação
Prostaglandinas/farmacologia
Prostaglandinas B/análise
Prostaglandinas B/química
Prostaglandinas B/isolamento & purificação
Prostaglandinas B/farmacologia
Espectrometria de Massas de Bombardeamento Rápido de Átomos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (Prostaglandins); 0 (Prostaglandins B); 22973-19-9 (15-ketoprostaglandin E); 31C4KY9ESH (Nitric Oxide); F5TD010360 (Alprostadil)
[Em] Mês de entrada:1102
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101015
[St] Status:MEDLINE
[do] DOI:10.1007/s12272-010-0905-y


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[PMID]:18825978
[Au] Autor:Kafanova TV; Busarova NG; Khudiakova IuV; Isai SV
[Ti] Título:[Marine fungus Stilbella aciculosa as a potential producer of prostaglandins].
[So] Source:Mikrobiologiia;77(4):508-11, 2008 Jul-Aug.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The amount and composition of fatty acids in the fungus Stilbella aciculosa associated with the marine macroorganism Apostichopus japonica (trepang) were determined by gas-liquid chromatography and gas chromatography-mass spectrometry. In the culture liquid of S. aciculosa, prostaglandins (PG) of groups E and F were revealed by UV spectroscopy. This finding was confirmed by the presence of direct precursors of PG, polyunsaturated eicosapentaenoic and docosahexaenoic acids, in the culture liquid. The biomass of this fungus contained PG of group B.
[Mh] Termos MeSH primário: Ascomicetos/metabolismo
Prostaglandinas B/biossíntese
Prostaglandinas E/biossíntese
Prostaglandinas F/biossíntese
[Mh] Termos MeSH secundário: Animais
Ascomicetos/crescimento & desenvolvimento
Ascomicetos/isolamento & purificação
Cromatografia Gasosa
Meios de Cultivo Condicionados/metabolismo
Ácidos Docosa-Hexaenoicos/análise
Ácidos Docosa-Hexaenoicos/metabolismo
Ácido Eicosapentaenoico/análise
Ácido Eicosapentaenoico/metabolismo
Cromatografia Gasosa-Espectrometria de Massas
Prostaglandinas B/isolamento & purificação
Prostaglandinas E/isolamento & purificação
Prostaglandinas F/isolamento & purificação
Stichopus/microbiologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media, Conditioned); 0 (Prostaglandins B); 0 (Prostaglandins E); 0 (Prostaglandins F); 25167-62-8 (Docosahexaenoic Acids); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:0811
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081002
[St] Status:MEDLINE


  4 / 169 MEDLINE  
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[PMID]:15231852
[Au] Autor:Little JM; Kurkela M; Sonka J; Jäntti S; Ketola R; Bratton S; Finel M; Radominska-Pandya A
[Ad] Endereço:Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
[Ti] Título:Glucuronidation of oxidized fatty acids and prostaglandins B1 and E2 by human hepatic and recombinant UDP-glucuronosyltransferases.
[So] Source:J Lipid Res;45(9):1694-703, 2004 Sep.
[Is] ISSN:0022-2275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arachidonic acid (AA) can be metabolized to various metabolites, which can act as mediators of cellular processes. The objective of this work was to identify whether AA, prostaglandin (PG) B1 and E2, and 15- and 20-hydroxyeicosatetraenoic acids (15- and 20-HETE) are metabolized via glucuronidation. Assays with human recombinant UDP-glucuronosyltransferase 1A (UGT1A) isoforms revealed that AA and 15-HETE were glucuronidated by UGT1A1, 1A3, 1A4, 1A9, and 1A10, whereas 20-HETE was glucuronidated by UGT1A1 and 1A4 and PGB1 was glucuronidated by UGT1A1, 1A9, and 1A10. All substrates were glucuronidated by recombinant UGT2B7, with AA and 20-HETE being the best substrates. Kinetic analysis of UGT1A1 and 1A9 with AA resulted in Km values of 37.9 and 45.8 microM, respectively. PGB1 was glucuronidated by UGT1A1 with a Km of 26.3 microM. The Km values for all substrates with UGT2B7 were significantly higher than with the UGT1A isoforms. Liquid chromatography-mass spectrometry of glucuronides biosynthesized from PGB1 and 15-HETE showed that hydroxyl groups were the major target of glucuronidation. This work demonstrates a novel metabolic pathway for HETEs and PGs and the role of UGT1A isoforms in this process. These results indicate that glucuronidation may play a significant role in modulation of the availability of these fatty acid derivatives for cellular processes.
[Mh] Termos MeSH primário: Dinoprostona/metabolismo
Ácidos Graxos/metabolismo
Glucuronídeos/metabolismo
Glucuronosiltransferase/metabolismo
Fígado/enzimologia
Prostaglandinas B/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cromatografia Líquida/métodos
Glucuronídeos/análise
Glucuronídeos/biossíntese
Seres Humanos
Ácidos Hidroxieicosatetraenoicos/química
Ácidos Hidroxieicosatetraenoicos/metabolismo
Intestinos/metabolismo
Cinética
Metabolismo dos Lipídeos
Espectrometria de Massas/métodos
Microssomos Hepáticos/metabolismo
Estrutura Molecular
Oxirredução
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Glucuronides); 0 (Hydroxyeicosatetraenoic Acids); 0 (Prostaglandins B); 0 (Recombinant Proteins); 1TYI1PJ64T (prostaglandin B1); EC 2.4.1.17 (Glucuronosyltransferase); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:0502
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040703
[St] Status:MEDLINE


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[PMID]:12734199
[Au] Autor:Silva J; Beckedorf A; Bieberich E
[Ad] Endereço:Institute of Molecular Medicine and Genetics, Department of Medicine, Medical College of Georgia, Augusta, Georgia 30909, USA.
[Ti] Título:Osteoblast-derived oxysterol is a migration-inducing factor for human breast cancer cells.
[So] Source:J Biol Chem;278(28):25376-85, 2003 Jul 11.
[Is] ISSN:0021-9258
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bone metastasis is the major reason for death caused by breast cancer. We used human breast cancer (MCF-7) cells that are poorly metastatic but show highly inducible migration to determine bone-derived factors that induce migration of initially non-disseminating breast cancer cells. We have found that a lipid fraction from human osteoblast-like MG63 cell-conditioned medium (MG63CM) contains a migration-inducing factor for MCF-7 cells. In this fraction, we have identified oxysterol (OS) as a lipid mediator for tumor cell migration. In MCF-7 cells, insulin-like growth factor 1 elevates the expression of OS-binding protein-related protein 7. Binding of OS to OS-binding protein or OS-binding protein-related protein is known to trigger elevation of sphingomyelin, a sphingolipid that organizes lipid microdomains in the cell membrane. In MCF-7 cells, OS increases the intracellular concentration of sphingomyelin and other phospholipids and induces the translocation of the small GTPase p21Ras to GM1- and cholesterol-rich membrane areas. The induction of migration by MG63CM is prevented by incubation of MG63 cells with mevinolin, a statin-type cholesterol biosynthesis inhibitor that depletes the conditioned medium of OS. Osteoblast-derived OS may, thus, be a yet unrecognized lipid mediator for bone metastasis of breast cancer and a new target for anti-metastasis chemotherapy with statins.
[Mh] Termos MeSH primário: Neoplasias da Mama/metabolismo
Metabolismo dos Lipídeos
Osteoblastos/metabolismo
Esteróis/metabolismo
[Mh] Termos MeSH secundário: Ácido Araquidônico/metabolismo
Aspirina/metabolismo
Membrana Celular/metabolismo
Movimento Celular
Colesterol/metabolismo
Cromatografia Líquida de Alta Pressão
Técnicas de Cocultura
Meios de Cultivo Condicionados/farmacologia
Dinoprostona/metabolismo
Eletroforese em Gel de Poliacrilamida
Proteínas de Choque Térmico HSP70/metabolismo
Seres Humanos
Fator de Crescimento Insulin-Like I/metabolismo
Lovastatina/farmacologia
Sistema de Sinalização das MAP Quinases
Microscopia de Fluorescência
Metástase Neoplásica
Fosfolipídeos/metabolismo
Fosforilação
Prostaglandinas B/metabolismo
Transporte Proteico
Proteínas Proto-Oncogênicas p21(ras)/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sefarose/farmacologia
Transdução de Sinais
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Esfingolipídeos/metabolismo
Tripsina/farmacologia
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Culture Media, Conditioned); 0 (HSP70 Heat-Shock Proteins); 0 (Phospholipids); 0 (Prostaglandins B); 0 (Sphingolipids); 0 (Sterols); 27YG812J1I (Arachidonic Acid); 67763-96-6 (Insulin-Like Growth Factor I); 9012-36-6 (Sepharose); 97C5T2UQ7J (Cholesterol); 9LHU78OQFD (Lovastatin); BIU61O9T9T (prostaglandin B2); EC 3.4.21.4 (Trypsin); EC 3.6.5.2 (HRAS protein, human); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras)); K7Q1JQR04M (Dinoprostone); R16CO5Y76E (Aspirin)
[Em] Mês de entrada:0308
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030508
[St] Status:MEDLINE


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[PMID]:11886778
[Au] Autor:Büyükgüzel K; Tunaz H; Putnam SM; Stanley D
[Ad] Endereço:Insect Biochemical Physiology Laboratory, 311 Plant Industry Building, University of Nebraska, Lincoln, NE 68583-0816, USA.
[Ti] Título:Prostaglandin biosynthesis by midgut tissue isolated from the tobacco hornworm, Manduca sexta.
[So] Source:Insect Biochem Mol Biol;32(4):435-43, 2002 Apr.
[Is] ISSN:0965-1748
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We describe prostaglandin (PG) biosynthesis by isolated midgut preparations from tobacco hornworms, Manduca sexta. Microsomal-enriched midgut preparations yielded four PGs, PGA/B(2), PGD(2), PGE(2) and PGF(2alpha), all of which were confirmed by analysis on gas chromatography--mass spectrometry (GC--MS). PGA and PGB are double bond isomers which do not resolve on TLC but do resolve by GC; for convenience, we use the single term PGA(2) for this product. PGA(2) was the major product under most conditions. The midgut preparations were sensitive to reaction conditions, including radioactive substrate, protein concentration (optimal at 1mg/reaction), reaction time (optimal at 0.5 min), temperature (optimal at 22 degrees C), buffer pH (highest at pH 6), and the presence of a co-factor cocktail composed of reduced glutathione, hydroquinine and hemoglobin. In vitro PG biosynthesis was inhibited by two cyclooxygenase inhibitors, indomethacin and naproxen. Subcellular localization of PG biosynthetic activity in midgut preparations, determined by ultracentrifugation, revealed the presence of PG biosynthetic activity in the cytosolic and microsomal fractions, although most activity was found in the cytosolic fractions. This is similar to other invertebrates, and different from mammalian preparations, in which the activity is exclusively associated with the microsomal fractions. Midgut preparations from M. sexta pupae, adult cockroach, Periplaneta americana, and corn ear worms, Helicoverpa zea, also produced the same four major PG products. We infer that insect midguts are competent to biosynthesize PGs, and speculate they exert important, albeit unrevealed, actions in midgut physiology.
[Mh] Termos MeSH primário: Manduca/metabolismo
Prostaglandinas/biossíntese
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/farmacologia
Técnicas de Cultura
Inibidores de Ciclo-Oxigenase/farmacologia
Sistema Digestório/efeitos dos fármacos
Sistema Digestório/metabolismo
Dinoprosta/biossíntese
Dinoprostona/biossíntese
Concentração de Íons de Hidrogênio
Indometacina/farmacologia
Proteínas de Insetos/metabolismo
Estrutura Molecular
Mariposas/metabolismo
Naproxeno/farmacologia
Prostaglandina D2/biossíntese
Prostaglandinas A/biossíntese
Prostaglandinas B/biossíntese
Frações Subcelulares
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cyclooxygenase Inhibitors); 0 (Insect Proteins); 0 (Prostaglandins); 0 (Prostaglandins A); 0 (Prostaglandins B); 57Y76R9ATQ (Naproxen); B7IN85G1HY (Dinoprost); BIU61O9T9T (prostaglandin B2); K6VT5BDY9E (prostaglandin A2); K7Q1JQR04M (Dinoprostone); RXY07S6CZ2 (Prostaglandin D2); XXE1CET956 (Indomethacin)
[Em] Mês de entrada:0205
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020312
[St] Status:MEDLINE


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[PMID]:11847277
[Au] Autor:Yang J; Petersen CE; Ha CE; Bhagavan NV
[Ad] Endereço:Department of Biochemistry and Biophysics, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA. bhagavan@hawaii.edu
[Ti] Título:Structural insights into human serum albumin-mediated prostaglandin catalysis.
[So] Source:Protein Sci;11(3):538-45, 2002 Mar.
[Is] ISSN:0961-8368
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies have shown that many arachidonic acid metabolites bind to human serum albumin (HSA) and that the metabolism of these molecules is altered as a result of binding. The present study attempted to gain insights into the mechanisms by which prostaglandins bound to subdomain 2A of HSA are metabolized by catalytic processes. The breakdown of the prostaglandin 15-keto-PGE(2) to 15-keto-PGA(2) and 15-keto-PGB(2) in the presence of wild-type HSA and a number of subdomain 2A mutants was examined using a previously validated spectroscopic method which monitors absorbance at 505 nm. The species examined using this method were wild-type HSA, K195M, K199M, F211V, W214L, R218M, R218P, R218H, R222M, H242V, R257M, and bovine serum albumin. Previous studies of HSA-mediated catalysis indicated that the breakdown of HSA-bound prostaglandins results from an alkaline microenvironment in the binding site. Our results show that the catalytic breakdown of HSA-bound 15-keto-PGE(2) to 15-keto-PGB(2) results from two specific processes which are modulated by specific amino acid residues. Specifically, some amino acid residues modulate the rate of step 1, the conversion of 15-keto-PGE(2) to 15-keto-PGA(2), while other residues modulate the rate of step 2, the conversion of 15-keto-PGA(2) to 15-keto-PGB(2). Some residues modulate the rate of steps 1 and 2. In total, while our results support the involvement of certain basic amino acid residues in the catabolism of HSA-bound 15-keto-PGE(2), our data suggest that metabolism of HSA-bound prostaglandins may be a more complex and specific process than previously thought.
[Mh] Termos MeSH primário: Dinoprostona/análogos & derivados
Dinoprostona/metabolismo
Albumina Sérica/metabolismo
[Mh] Termos MeSH secundário: Catálise
Clonagem Molecular
Seres Humanos
Concentração de Íons de Hidrogênio
Mutagênese Sítio-Dirigida
Prostaglandinas B/metabolismo
Estrutura Terciária de Proteína
Albumina Sérica/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Prostaglandins B); 0 (Serum Albumin); 2S0F1FTK13 (15-ketoprostaglandin E2); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:0209
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020216
[St] Status:MEDLINE


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[PMID]:10993336
[Au] Autor:Mikolajczyk M; Mikina M; Jankowiak A
[Ad] Endereço:Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódz, Sienkiewicza. marmikol@bilbo.cbmm.lodz.pl
[Ti] Título:A novel synthesis of racemic and enantiomeric forms of prostaglandin B1 methyl ester.
[So] Source:J Org Chem;65(17):5127-30, 2000 Aug 25.
[Is] ISSN:0022-3263
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:3-(Dimethoxyphosphorylmethyl)cyclopent-2-enone was converted into (+/-)-prostaglandin B1 methyl ester in two steps involving regioselective alkylation at C(2) with methyl 7-iodoheptanoate and subsequent Horner-Wittig reaction with dimer of 2-hydroxyheptanal (42% overall yield). The use of (R)- and (S)-2-(tert-butyldimethylsilyloxy)heptanal for the Horner olefination reaction gave, after deprotection of the hydroxy group, the enantiopure forms of the title compound in 28% overall yield.
[Mh] Termos MeSH primário: Prostaglandinas B/síntese química
[Mh] Termos MeSH secundário: Ésteres/química
Espectroscopia de Ressonância Magnética
Prostaglandinas B/química
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Esters); 0 (Prostaglandins B); 1TYI1PJ64T (prostaglandin B1)
[Em] Mês de entrada:0010
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000919
[St] Status:MEDLINE


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[PMID]:10903809
[Au] Autor:Cattan N; Mary D; Peleraux A; Mari B; Aussel C; Rossi B
[Ad] Endereço:INSERM U. 364, IFR50, Faculté de Médecine Pasteur, avenue de Valombrose, 06107 Nice Cedex 02, France.
[Ti] Título:Prostaglandin B(2) delivers a co-stimulatory signal leading to T cell activation.
[So] Source:Eur Cytokine Netw;11(2):293-9, 2000 Jun.
[Is] ISSN:1148-5493
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Most of the data accumulated to date on the immunoregulatory effects of prostaglandins (PG) on T cell activation stem from the archetypal inhibitory effect of PGE(2). In this study we provide instead, the first evidence that exogenous PGB(2), a catabolic metabolite of PGE(2), synergizes with signals delivered by T cell receptor (TCR) engagement to induce interleukin-2 (IL-2) production and IL-2 receptor (IL-2R) alpha-expression in Jurkat cells. Accordingly, PGB(2) enhances the proliferation of anti-CD3-activated peripheral blood lymphocytes (PBL). In terms of cellular signaling, we present evidence that PGB(2) activates tyrosine kinase activities and efficiently increases c-fos mRNA expression and nuclear factor-kappa B (NF-kappa B) translocation to the nucleus. Owing to these features, PGB(2) appears as a new lipid mediator capable of delivering an ancillary signal leading to T lymphocyte activation.
[Mh] Termos MeSH primário: Ativação Linfocitária/efeitos dos fármacos
Prostaglandinas B/farmacologia
Linfócitos T/efeitos dos fármacos
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Antígenos CD/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Divisão Celular/efeitos dos fármacos
Ativação Enzimática/efeitos dos fármacos
Genes fos/efeitos dos fármacos
Seres Humanos
Técnicas In Vitro
Interleucina-2/biossíntese
Interleucina-2/genética
Células Jurkat
Lectinas Tipo C
NF-kappa B/metabolismo
Proteínas Tirosina Quinases/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores de Antígenos de Linfócitos T/efeitos dos fármacos
Receptores de Antígenos de Linfócitos T/metabolismo
Receptores de Interleucina-2/genética
Receptores de Interleucina-2/metabolismo
Transdução de Sinais
Linfócitos T/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (Interleukin-2); 0 (Lectins, C-Type); 0 (NF-kappa B); 0 (Prostaglandins B); 0 (RNA, Messenger); 0 (Receptors, Antigen, T-Cell); 0 (Receptors, Interleukin-2); BIU61O9T9T (prostaglandin B2); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:0009
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000721
[St] Status:MEDLINE


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[PMID]:10672021
[Au] Autor:Tassin-Moindrot S; Caille A; Douliez JP; Marion D; Vovelle F
[Ad] Endereço:Centre de Biophysique Moléculaire, UPR 4301 CNRS, Orléans, France; Université d'Orléans, France.
[Ti] Título:The wide binding properties of a wheat nonspecific lipid transfer protein. Solution structure of a complex with prostaglandin B2.
[So] Source:Eur J Biochem;267(4):1117-24, 2000 Feb.
[Is] ISSN:0014-2956
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 3D solution structure of wheat nonspecific lipid transfer protein (ns-LTP) complexed with prostaglandin B2, a lipid with both vinyl and hydroxylated groups, has been determined by 1H 2D NMR. The global fold of the protein is close to the previously published structures of wheat, maize, barley and rice ns-LTPs. The ligand is almost completely embedded in the hydrophobic core of the protein. Structure comparisons of free and bound wheat ns-LTP reveal that the binding of prostaglandin B2 hardly affects the global fold of the protein. The structural data on this unusual complex are discussed and compared with other known ns-LTP lipid-complexes.
[Mh] Termos MeSH primário: Proteínas de Transporte/química
Proteínas de Transporte/metabolismo
Prostaglandinas B/metabolismo
Triticum/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Ácidos Graxos/metabolismo
Ligantes
Modelos Moleculares
Ressonância Magnética Nuclear Biomolecular
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Prostaglandinas B/química
Ligação Proteica
Conformação Proteica
Dobramento de Proteína
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Fatty Acids); 0 (Ligands); 0 (Plant Proteins); 0 (Prostaglandins B); 0 (Solutions); 0 (sterol carrier proteins); BIU61O9T9T (prostaglandin B2)
[Em] Mês de entrada:0003
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000215
[St] Status:MEDLINE



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