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[PMID]:28923202
[Au] Autor:Warner DR; Liu H; Miller ME; Ramsden CE; Gao B; Feldstein AE; Schuster S; McClain CJ; Kirpich IA
[Ad] Endereço:Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky.
[Ti] Título:Dietary Linoleic Acid and Its Oxidized Metabolites Exacerbate Liver Injury Caused by Ethanol via Induction of Hepatic Proinflammatory Response in Mice.
[So] Source:Am J Pathol;187(10):2232-2245, 2017 Oct.
[Is] ISSN:1525-2191
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alcoholic liver disease is a major human health problem leading to significant morbidity and mortality in the United States and worldwide. Dietary fat plays an important role in alcoholic liver disease pathogenesis. Herein, we tested the hypothesis that a combination of ethanol and a diet rich in linoleic acid (LA) leads to the increased production of oxidized LA metabolites (OXLAMs), specifically 9- and 13-hydroxyoctadecadienoic acids (HODEs), which contribute to a hepatic proinflammatory response exacerbating liver injury. Mice were fed unsaturated (with a high LA content) or saturated fat diets (USF and SF, respectively) with or without ethanol for 10 days, followed by a single binge of ethanol. Compared to SF+ethanol, mice fed USF+ethanol had elevated plasma alanine transaminase levels, enhanced hepatic steatosis, oxidative stress, and inflammation. Plasma and liver levels of 9- and 13-HODEs were increased in response to USF+ethanol feeding. We demonstrated that primarily 9-HODE, but not 13-HODE, induced the expression of several proinflammatory cytokines in vitro in RAW264.7 macrophages. Finally, deficiency of arachidonate 15-lipoxygenase, a major enzyme involved in LA oxidation and OXLAM production, attenuated liver injury and inflammation caused by USF+ethanol feeding but had no effect on hepatic steatosis. This study demonstrates that OXLAM-mediated induction of a proinflammatory response in macrophages is one of the potential mechanisms underlying the progression from alcohol-induced steatosis to alcoholic steatohepatitis.
[Mh] Termos MeSH primário: Gorduras na Dieta/efeitos adversos
Inflamação/patologia
Ácido Linoleico/efeitos adversos
Fígado/metabolismo
Fígado/patologia
[Mh] Termos MeSH secundário: Animais
Araquidonato 15-Lipoxigenase/metabolismo
Bebedeira
Composição Corporal
Citocinas/metabolismo
Modelos Animais de Doenças
Etanol
Ácidos Linoleicos/metabolismo
Ácidos Linoleicos Conjugados/metabolismo
Macrófagos/metabolismo
Metaboloma
Camundongos
Camundongos Endogâmicos C57BL
Oxirredução
Estresse Oxidativo
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Dietary Fats); 0 (Linoleic Acids); 0 (Linoleic Acids, Conjugated); 15514-85-9 (9-hydroxy-10,12-octadecadienoic acid); 3K9958V90M (Ethanol); 5204-88-6 (13-hydroxy-9,11-octadecadienoic acid); 9KJL21T0QJ (Linoleic Acid); EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28346333
[Au] Autor:Veselinovic M; Vasiljevic D; Vucic V; Arsic A; Petrovic S; Tomic-Lucic A; Savic M; Zivanovic S; Stojic V; Jakovljevic V
[Ad] Endereço:Department of Internal medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac 34000, Serbia. veselinovic.m@sbb.rs.
[Ti] Título:Clinical Benefits of n-3 PUFA and ɤ-Linolenic Acid in Patients with Rheumatoid Arthritis.
[So] Source:Nutrients;9(4), 2017 Mar 25.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:(1) Background: Marine -3 polyunsaturated fatty acids (PUFA) and ɤ-linolenic acid (GLA) are well-known anti-inflammatory agents that may help in the treatment of inflammatory disorders. Their effects were examined in patients with rheumatoid arthritis; (2) Methods: Sixty patients with active rheumatoid arthritis were involved in a prospective, randomized trial of a 12 week supplementation with fish oil (group I), fish oil with primrose evening oil (group II), or with no supplementation (group III). Clinical and laboratory evaluations were done at the beginning and at the end of the study; (3) Results: The Disease Activity Score 28 (DAS 28 score), number of tender joints and visual analogue scale (VAS) score decreased notably after supplementation in groups I and II ( < 0.001). In plasma phospholipids the -6/ -3 fatty acids ratio declined from 15.47 ± 5.51 to 10.62 ± 5.07 ( = 0.005), and from 18.15 ± 5.04 to 13.50 ± 4.81 ( = 0.005) in groups I and II respectively. The combination of -3 PUFA and GLA (group II) increased ɤ-linolenic acid (0.00 ± 0.00 to 0.13 ± 0.11, < 0.001), which was undetectable in all groups before the treatments; (4) Conclusion: Daily supplementation with -3 fatty acids alone or in combination with GLA exerted significant clinical benefits and certain changes in disease activity.
[Mh] Termos MeSH primário: Artrite Reumatoide/tratamento farmacológico
Ácidos Graxos Ômega-3/administração & dosagem
Ácido alfa-Linolênico/administração & dosagem
[Mh] Termos MeSH secundário: Idoso
Anti-Inflamatórios/administração & dosagem
Ácido Araquidônico/administração & dosagem
Ácido Araquidônico/sangue
Artrite Reumatoide/sangue
Suplementos Nutricionais
Método Duplo-Cego
Ácido Eicosapentaenoico/administração & dosagem
Ácido Eicosapentaenoico/sangue
Ácidos Graxos Ômega-3/sangue
Ácidos Graxos Ômega-6/sangue
Feminino
Óleos de Peixe/administração & dosagem
Seres Humanos
Ácidos Linoleicos/administração & dosagem
Ácidos Linoleicos/sangue
Meia-Idade
Fosfolipídeos/sangue
Óleos Vegetais/administração & dosagem
Estudos Prospectivos
Resultado do Tratamento
Ácido alfa-Linolênico/sangue
Ácido gama-Linolênico/administração & dosagem
Ácido gama-Linolênico/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Fatty Acids, Omega-3); 0 (Fatty Acids, Omega-6); 0 (Fish Oils); 0 (Linoleic Acids); 0 (Phospholipids); 0 (Plant Oils); 0RBV727H71 (alpha-Linolenic Acid); 27YG812J1I (Arachidonic Acid); 3Q9L08K71N (evening primrose oil); 78YC2MAX4O (gamma-Linolenic Acid); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


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[PMID]:28264934
[Au] Autor:Chen J; De Raeymaecker J; Hovgaard JB; Smaardijk S; Vandecaetsbeek I; Wuytack F; Møller JV; Eggermont J; De Maeyer M; Christensen SB; Vangheluwe P
[Ad] Endereço:From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
[Ti] Título:Structure/activity relationship of thapsigargin inhibition on the purified Golgi/secretory pathway Ca /Mn -transport ATPase (SPCA1a).
[So] Source:J Biol Chem;292(17):6938-6951, 2017 Apr 28.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Golgi/secretory pathway Ca /Mn -transport ATPase (SPCA1a) is implicated in breast cancer and Hailey-Hailey disease. Here, we purified recombinant human SPCA1a from and measured Ca -dependent ATPase activity following reconstitution in proteoliposomes. The purified SPCA1a displays a higher apparent Ca affinity and a lower maximal turnover rate than the purified sarco(endo)plasmic reticulum Ca -ATPase (SERCA1a). The lipids cholesteryl hemisuccinate, linoleamide/oleamide, and phosphatidylethanolamine inhibit and phosphatidic acid and sphingomyelin enhance SPCA1a activity. Moreover, SPCA1a is blocked by micromolar concentrations of the commonly used SERCA1a inhibitors thapsigargin (Tg), cyclopiazonic acid, and 2,5-di- -butylhydroquinone. Because tissue-specific targeting of SERCA2b by Tg analogues is considered for prostate cancer therapy, the inhibition of SPCA1a by Tg might represent an off-target risk. We assessed the structure-activity relationship (SAR) of Tg for SPCA1a by modeling, site-directed mutagenesis, and measuring the potency of a series of Tg analogues. These indicate that Tg and the analogues are bound via the Tg scaffold but with lower affinity to the same homologous cavity as on the membrane surface of SERCA1a. The lower Tg affinity may depend on a more flexible binding cavity in SPCA1a, with low contributions of the Tg O-3, O-8, and O-10 chains to the binding energy. Conversely, the protein interaction of the Tg O-2 side chain with SPCA1a appears comparable with that of SERCA1a. These differences define a SAR of Tg for SPCA1a distinct from that of SERCA1a, indicating that Tg analogues with a higher specificity for SPCA1a can probably be developed.
[Mh] Termos MeSH primário: ATPases Transportadoras de Cálcio/antagonistas & inibidores
ATPases Transportadoras de Cálcio/química
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores
Tapsigargina/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/uso terapêutico
Neoplasias da Mama/tratamento farmacológico
Cálcio/química
Colesterol/química
Desenho de Drogas
Feminino
Seres Humanos
Hidroquinonas/química
Indóis/química
Ácidos Linoleicos/química
Lipossomos/química
Masculino
Mutagênese Sítio-Dirigida
Ácidos Oleicos/química
Ácidos Fosfatídicos/química
Neoplasias da Próstata/tratamento farmacológico
Ligação Proteica
Conformação Proteica
Coelhos
Proteínas Recombinantes/química
Saccharomyces cerevisiae/metabolismo
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/química
Esfingomielinas/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Hydroquinones); 0 (Indoles); 0 (Linoleic Acids); 0 (Liposomes); 0 (Oleic Acids); 0 (Phosphatidic Acids); 0 (Recombinant Proteins); 0 (Sphingomyelins); 26XK13B61B (2,5-di-tert-butylhydroquinone); 5340S2UXSP (linoleamide); 67526-95-8 (Thapsigargin); 7L25QK8BWO (oleylamide); 97C5T2UQ7J (Cholesterol); EC 3.6.3.8 (ATP2A2 protein, human); EC 3.6.3.8 (ATP2C1 protein, human); EC 3.6.3.8 (Calcium-Transporting ATPases); EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases); SY7Q814VUP (Calcium); X9TLY4580Z (cyclopiazonic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.778431


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[PMID]:28182745
[Au] Autor:Bubici G; Carluccio AV; Stavolone L; Cillo F
[Ad] Endereço:Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Bari, Italy.
[Ti] Título:Prosystemin overexpression induces transcriptional modifications of defense-related and receptor-like kinase genes and reduces the susceptibility to Cucumber mosaic virus and its satellite RNAs in transgenic tomato plants.
[So] Source:PLoS One;12(2):e0171902, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Systemin is a plant signal peptide hormone involved in the responses to wounding and insect damage in the Solanaceae family. It works in the same signaling pathway of jasmonic acid (JA) and enhances the expression of proteinase inhibitors. With the aim of studying a role for systemin in plant antiviral responses, a tomato (Solanum lycopersicum) transgenic line overexpressing the prosystemin cDNA, i.e. the systemin precursor, was inoculated with Cucumber mosaic virus (CMV) strain Fny supporting either a necrogenic or a non-necrogenic satellite RNA (satRNA) variant. Transgenic plants showed reduced susceptibility to both CMV/satRNA combinations. While symptoms of the non-necrogenic inoculum were completely suppressed, a delayed onset of lethal disease occurred in about half of plants challenged with the necrogenic inoculum. RT-qPCR analysis showed a correlation between the systemin-mediated reduced susceptibility and the JA biosynthetic and signaling pathways (e.g. transcriptional alteration of lipoxygenase D and proteinase inhibitor II). Moreover, transgenically overexpressed systemin modulated the expression of a selected set of receptor-like protein kinase (RLK) genes, including some playing a known role in plant innate immunity. A significant correlation was found between the expression profiles of some RLKs and the systemin-mediated reduced susceptibility to CMV/satRNA. These results show that systemin can increase plant defenses against CMV/satRNA through transcriptional reprogramming of diverse signaling pathways.
[Mh] Termos MeSH primário: Cucumovirus/patogenicidade
Lycopersicon esculentum/imunologia
Peptídeos/genética
Imunidade Vegetal
Receptores Proteína Tirosina Quinases/genética
[Mh] Termos MeSH secundário: Cucumovirus/genética
Regulação da Expressão Gênica de Plantas
Ácidos Linoleicos/metabolismo
Lipoxigenase/genética
Lipoxigenase/metabolismo
Lycopersicon esculentum/genética
Lycopersicon esculentum/virologia
Peptídeos/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
RNA Satélite/genética
Receptores Proteína Tirosina Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Linoleic Acids); 0 (Peptides); 0 (Plant Proteins); 0 (RNA, Satellite); 0 (jasminic acid); 0 (proteinase inhibitor II protein, plant); 360F030D37 (systemin); EC 1.13.11.12 (Lipoxygenase); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171902


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[PMID]:28124089
[Au] Autor:Mosaad SM; Zaitone SA; Ahmed AA; Abo-Elmatty DM; El-Baz AA; Moustafa YM
[Ad] Endereço:Department of Pharmaceutical Inspection, Ministry of Health, Ismailia, 41111, Egypt.
[Ti] Título:Evening primrose oil or forskolin ameliorates celecoxib-enhanced upregulation of tissue factor expression in mice subjected to lipopolysaccharide-induced endotoxemia.
[So] Source:Naunyn Schmiedebergs Arch Pharmacol;390(5):483-492, 2017 May.
[Is] ISSN:1432-1912
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Celecoxib, a selective cyclooxygenase-2 inhibitor, produces thrombotic events in patients predisposed to cardiovascular risk factors. One theory reported an increase in endothelial expression of tissue factor (TF) as a predisposing factor. This work explored the effect of evening primrose oil (EPO), a source of prostaglandin E1, and forskolin (a cyclic adenosine monophosphate stimulator) against the prothrombotic effect of celecoxib in mice. Lipopolysaccharide mouse model of endotoxemia was used to induce an upregulation of TF activity. Male mice received celecoxib (25 mg/kg), celecoxib plus EPO, or celecoxib plus forskolin for 4 weeks and then subjected to a prothrombotic challenge in the form of an intraperitoneal injection of lipopolysaccharide. Results showed an increase in plasma TF activity, endothelial TF expression, and thrombin-antithrombin (TAT) but lower antithrombin III (ATIII) level in mice that received celecoxib in comparison to those that received the vehicle. Adding EPO or forskolin to celecoxib regimen significantly decreased the prothrombotic effect of celecoxib. A positive correlation (r = 0.8501) was found between TF activity and TAT. Co-administration of EPO or forskolin decreased the activity of TF and mitigated the prothrombotic effect of celecoxib. Therefore, these combinations may have the utility to abrogate the prothrombotic adverse effect of celecoxib in clinical setting.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Celecoxib
Colforsina/farmacologia
Inibidores de Ciclo-Oxigenase 2
Endotoxemia/induzido quimicamente
Fibrinolíticos/farmacologia
Ácidos Linoleicos/farmacologia
Lipopolissacarídeos
Óleos Vegetais/farmacologia
Tromboplastina/metabolismo
Trombose/prevenção & controle
Ácido gama-Linolênico/farmacologia
[Mh] Termos MeSH secundário: Animais
Antitrombina III/metabolismo
Modelos Animais de Doenças
Endotoxemia/sangue
Pulmão/efeitos dos fármacos
Pulmão/metabolismo
Masculino
Camundongos
Peptídeo Hidrolases/sangue
Trombose/sangue
Trombose/induzido quimicamente
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclooxygenase 2 Inhibitors); 0 (Fibrinolytic Agents); 0 (Linoleic Acids); 0 (Lipopolysaccharides); 0 (Plant Oils); 0 (antithrombin III-protease complex); 0 (lipopolysaccharide, Escherichia coli O111 B4); 1F7A44V6OU (Colforsin); 3Q9L08K71N (evening primrose oil); 78YC2MAX4O (gamma-Linolenic Acid); 9000-94-6 (Antithrombin III); 9035-58-9 (Thromboplastin); EC 3.4.- (Peptide Hydrolases); JCX84Q7J1L (Celecoxib)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1007/s00210-017-1342-y


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[PMID]:28109294
[Au] Autor:Muralikumar S; Vetrivel U; Narayanasamy A; N Das U
[Ad] Endereço:Centre for Bioinformatics, Kamalnayan Bajaj Institute for Research in Vision and Ophthalmology, Vision Research Foundation, Sankara Nethralaya, Chennai, 600 006, Tamil Nadu, India.
[Ti] Título:Probing the intermolecular interactions of PPARγ-LBD with polyunsaturated fatty acids and their anti-inflammatory metabolites to infer most potential binding moieties.
[So] Source:Lipids Health Dis;16(1):17, 2017 Jan 21.
[Is] ISSN:1476-511X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: PPARγ is an isoform of peroxisome proliferator-activated receptor (PPAR) belonging to a super family of nuclear receptors. PPARγ receptor is found to play a crucial role in the modulation of lipid and glucose homeostasis. Its commotion has been reported to play a significant role in a broad spectrum of diseases such as type 2 diabetes mellitus, inflammatory diseases, Alzheimer's disease, and in some cancers. Hence, PPARγ is an important therapeutic target. Polyunsaturated fatty acids (PUFAs) and their metabolites (henceforth referred to as bioactive lipids) are known to function as agonists of PPARγ. However, agonistic binding modes and affinity of these ligands to PPARγ are yet to be deciphered. METHODS: In this study, we performed a comparative molecular docking, binding free energy calculation and molecular dynamics simulation to infer and rank bioactive lipids based on the binding affinities with the ligand binding domain (LBD) of PPARγ. RESULTS: The results inferred affinity in the order of resolvin E1 > neuroprotectin D1 > hydroxy-linoleic acid > docosahexaenoic acid > lipoxin A4 > gamma-linolenic acid, arachidonic acid > alpha-linolenic acid > eicosapentaenoic acid > linoleic acid. Of all the bioactive lipids studied, resolvin E1, neuroprotectin D1 and hydroxy-linoleic acid showed significant affinity comparable to proven PPARγ agonist namely, rosiglitazone, in terms of Glide XP docking score, H-bond formation with the key residues, binding free energy and stable complex formation with LBD favouring co-activator binding, as inferred through Molecular Dynamics trajectory analysis. CONCLUSION: Hence, these three bioactive lipids (resolvin E1, neuroprotectin D1 and hydroxy-linoleic acid) may be favourably considered as ideal drug candidates in therapeutic modulation of clinical conditions such as type 2 DM, Alzheimer's disease and other instances where PPARγ is a key player.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Ácidos Docosa-Hexaenoicos/química
Ácido Eicosapentaenoico/análogos & derivados
Ácidos Linoleicos/química
Simulação de Acoplamento Molecular
PPAR gama/química
[Mh] Termos MeSH secundário: Anti-Inflamatórios/metabolismo
Ácido Araquidônico/química
Ácido Araquidônico/metabolismo
Sítios de Ligação
Ácidos Docosa-Hexaenoicos/metabolismo
Ácido Eicosapentaenoico/química
Ácido Eicosapentaenoico/metabolismo
Seres Humanos
Cinética
Ácido Linoleico/química
Ácido Linoleico/metabolismo
Ácidos Linoleicos/metabolismo
Lipoxinas/química
Lipoxinas/metabolismo
Simulação de Dinâmica Molecular
PPAR gama/metabolismo
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Estrutura Secundária de Proteína
Termodinâmica
Tiazolidinedionas/química
Tiazolidinedionas/metabolismo
Ácido alfa-Linolênico/química
Ácido alfa-Linolênico/metabolismo
Ácido gama-Linolênico/química
Ácido gama-Linolênico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid); 0 (Anti-Inflammatory Agents); 0 (Linoleic Acids); 0 (Lipoxins); 0 (PPAR gamma); 0 (Thiazolidinediones); 0 (lipoxin A4); 0 (protectin D1); 05V02F2KDG (rosiglitazone); 0RBV727H71 (alpha-Linolenic Acid); 102622-88-8 (8-hydroxylinoleic acid); 25167-62-8 (Docosahexaenoic Acids); 27YG812J1I (Arachidonic Acid); 78YC2MAX4O (gamma-Linolenic Acid); 9KJL21T0QJ (Linoleic Acid); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE
[do] DOI:10.1186/s12944-016-0404-3


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[PMID]:27886543
[Au] Autor:Wang L; Yi W; Ye J; Qin H; Long Y; Yang M; Li Q
[Ad] Endereço:Key Laboratory of Environmental Exposure and Health of Guangzhou City, School of Environment, Jinan University, Guangzhou, 510632, Guangdong, China; Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, 94598, CA, USA.
[Ti] Título:Interactions among triphenyltin degradation, phospholipid synthesis and membrane characteristics of Bacillus thuringiensis in the presence of d-malic acid.
[So] Source:Chemosphere;169:403-412, 2017 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Degradation pathway and surface biosorption of triphenyltin (TPT) by effective microbes have been investigated in the past. However, unclear interactions among membrane components and TPT binding and transport are still obstacles to understanding TPT biotransformation. To reveal the mechanism involved, the phospholipid expression, membrane potential, cellular mechanism and molecular dynamics between TPT and fatty acids (FAs) during the TPT degradation process in the presence of d-malic acid (DMA) were studied. The results show that the degradation efficiency of 1 mg L TPT by Bacillus thuringiensis (1 g L ) with 0.5 or 1 mg L DMA reached values up to approximately 90% due to the promotion of element metabolism and cellular activity, and the depression of FA synthesis induced by DMA. The addition of DMA caused conversion of more linoleic acid into 10-oxo-12(Z)-octadecenoic acid, increased the membrane permeability, and alleviated the decrease in membrane potential, resulting in TPT transport and degradation. Fluorescence analysis reveals that the endospore of B. thuringiensis could act as an indicator for membrane potential and cellular activities. The current findings are advantageous for acceleration of biosorption, transport and removal of pollutants from natural environments.
[Mh] Termos MeSH primário: Bacillus thuringiensis/metabolismo
Permeabilidade da Membrana Celular/fisiologia
Membrana Celular/metabolismo
Malatos/farmacologia
Potenciais da Membrana/fisiologia
Compostos Orgânicos de Estanho/metabolismo
Fosfolipídeos/biossíntese
[Mh] Termos MeSH secundário: Fluorescência
Ácido Linoleico/metabolismo
Ácidos Linoleicos/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (10-oxo-12-octadecenoic acid); 0 (Linoleic Acids); 0 (Malates); 0 (Organotin Compounds); 0 (Phospholipids); 817L1N4CKP (malic acid); 95T92AGN0V (triphenyltin); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


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[PMID]:27863255
[Au] Autor:Toporkova YY; Fatykhova VS; Gogolev YV; Khairutdinov BI; Mukhtarova LS; Grechkin AN
[Ad] Endereço:Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, Kazan 420111, Russia.
[Ti] Título:Epoxyalcohol synthase of Ectocarpus siliculosus. First CYP74-related enzyme of oxylipin biosynthesis in brown algae.
[So] Source:Biochim Biophys Acta;1862(2):167-175, 2017 02.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Enzymes of CYP74 family play the central role in the biosynthesis of physiologically important oxylipins in land plants. Although a broad diversity of oxylipins is known in the algae, no CYP74s or related enzymes have been detected in brown algae yet. Cloning of the first CYP74-related gene CYP5164B1 of brown alga Ectocarpus siliculosus is reported in present work. The recombinant protein was incubated with several fatty acid hydroperoxides. Linoleic acid 9-hydroperoxide (9-HPOD) was the preferred substrate, while linoleate 13-hydroperoxide (13-HPOD) was less efficient. α-Linolenic acid 9- and 13-hydroperoxides, as well as eicosapentaenoic acid 15-hydroperoxide were inefficient substrates. Both 9-HPOD and 13-HPOD were converted into epoxyalcohols. For instance, 9-HPOD was turned primarily into (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid. Both epoxide and hydroxyl oxygen atoms of the epoxyalcohol were incorporated mostly from [ O ]9-HPOD. Thus, the enzyme exhibits the activity of epoxyalcohol synthase (EsEAS). The results show that the EsEAS isomerizes the hydroperoxides into epoxyalcohols via epoxyallylic radical, a common intermediate of different CYP74s and related enzymes. EsEAS can be considered as an archaic prototype of CYP74 family enzymes.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Compostos de Epóxi/metabolismo
Oxilipinas/metabolismo
Feófitas/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Peróxido de Hidrogênio/metabolismo
Ácidos Linoleicos/metabolismo
Peróxidos Lipídicos/metabolismo
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Especificidade por Substrato
Ácido alfa-Linolênico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (9-hydroperoxy-10,12-octadecadienoic acid); 0 (Epoxy Compounds); 0 (Linoleic Acids); 0 (Lipid Peroxides); 0 (Oxylipins); 0 (Plant Proteins); 0 (Recombinant Proteins); 0RBV727H71 (alpha-Linolenic Acid); 23017-93-8 (13-hydroperoxy-9,11-octadecadienoic acid); 9035-51-2 (Cytochrome P-450 Enzyme System); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  9 / 6559 MEDLINE  
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[PMID]:27752771
[Au] Autor:Balen M; Gomes GR; Kratz JM; Simões CM; Valério A; de Oliveira D
[Ad] Endereço:Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
[Ti] Título:Enzymatic synthesis of ascorbyl ester derived from linoleic acid.
[So] Source:Bioprocess Biosyst Eng;40(2):265-270, 2017 Feb.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Antioxidants are substances that defend cells against damage, kidnapping and destroying free radicals. They have been largely used in the food industry due the possibility to control the oxidation process, aimed to increase shelf life. Thus, esterification reaction to obtain ascorbyl linoleate catalyzed by Novozym 435 lipase assisted by ultrasound bath was investigated. In this work, molecular sieve (4 Å) was added to the reaction medium to remove the water formed during the esterification reaction to improve the process performance. According to the results, ascorbyl linoleate production up to 90 % was reached after 1 h of reaction time carried out using ultrasound bath, 1:9 molar ratio of substrates L-ascorbic acid to linoleic acid, 20 mL of tert-butanol as organic solvent, 5 wt% of Novozym 435 lipase, 10 wt% of molecular sieve at 70 °C.
[Mh] Termos MeSH primário: Ácido Ascórbico/análogos & derivados
Ácidos Linoleicos/síntese química
Lipase/química
[Mh] Termos MeSH secundário: Ácido Ascórbico/síntese química
Ácido Ascórbico/química
Ácidos Linoleicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Linoleic Acids); 0 (ascorbyl linoleate); EC 3.1.1.- (Novozyme 435); EC 3.1.1.3 (Lipase); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-016-1694-6


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[PMID]:27595606
[Au] Autor:Yamamoto S; Takehara M; Ushimaru M
[Ad] Endereço:Department of Chemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan. Electronic address: s_yamamoto@ks.kyorin-u.ac.jp.
[Ti] Título:Inhibitory action of linoleamide and oleamide toward sarco/endoplasmic reticulum Ca -ATPase.
[So] Source:Biochim Biophys Acta;1861(1 Pt A):3399-3405, 2017 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: SERCA maintains intracellular Ca homeostasis by sequestering cytosolic Ca into SR/ER stores. Two primary fatty acid amides (PFAAs), oleamide (18:1 ) and linoleamide (18:2 ), induce an increase in intracellular Ca levels, which might be caused by their inhibition of SERCA. METHODS: Three major SERCA isoforms, rSERCA1a, hSERCA2b, and hSERCA3a, were individually overexpressed in COS-1 cells, and the inhibitory action of PFAAs on Ca -ATPase activity of SERCA was examined. RESULTS: The Ca -ATPase activity of each SERCA was inhibited in a concentration-dependent manner strongly by linoleamide (IC 15-53µM) and partially by oleamide (IC 8.3-34µM). Inhibition by other PFAAs, such as stearamide (18:0) and elaidamide (18:1 ), was hardly or slightly observed. With increasing dose, linoleamide decreased the apparent affinity for Ca and the apparent maximum velocity of Ca -ATPase activity of all SERCAs tested. Oleamide also lowered these values for hSERCA3a. Meanwhile, oleamide uniquely reduced the apparent Ca affinity of rSERCA1a and hSERCA2b: the reduction was considerably attenuated above certain concentrations of oleamide. The dissociation constants for SERCA interaction varied from 6 to 45µM in linoleamide and from 1.6 to 55µM in oleamide depending on the isoform. CONCLUSIONS: Linoleamide and oleamide inhibit SERCA activity in the micromolar concentration range, and in a different manner. Both amides mainly suppress SERCA activity by lowering the Ca affinity of the enzyme. GENERAL SIGNIFICANCE: Our findings imply a novel role of these PFAAs as modulators of intracellular Ca homeostasis via regulation of SERCA activity.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Ácidos Linoleicos/farmacologia
Ácidos Oleicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Isoenzimas/antagonistas & inibidores
Isoenzimas/metabolismo
Cinética
Coelhos
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Isoenzymes); 0 (Linoleic Acids); 0 (Oleic Acids); 5340S2UXSP (linoleamide); 7L25QK8BWO (oleylamide); EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE



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