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[PMID]:29367479
[Au] Autor:Pornputtapitak W; Pantakitcharoenkul J; Panpakdee R; Teeranachaideekul V; Sinchaipanid N
[Ad] Endereço:Department of Chemical Engineering, Faculty of Engineering, Mahidol University.
[Ti] Título:Development of γ-Oryzanol Rich Extract from Leum Pua Glutinous Rice Bran Loaded Nanostructured Lipid Carriers for Topical Delivery.
[So] Source:J Oleo Sci;67(2):125-133, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Leum Pua is native Thai glutinous rice that contains antioxidants higher than white rice and other colored rice. One of the major antioxidants in rice brans is γ-oryzanol (GO). In this study, Leum Pua glutinous rice bran was extracted by different solvents. Oleic acid (~40 g/100 g extract), linoleic acid (~30 g/100 g extract), and palmitic acid (~20 g/100 g extract) were found to be major lipid components in the extracts. Methanol extract showed less variety of lipid components compared to the others. However, hexane extract showed the highest percent of γ-oryzanol compared to other solvents. Therefore, the hexane extract was selected to prepare nanostructured lipid carriers (NLC). The prepared NLC had small particles in the size range of 142.9 ± 0.4 nm for extract-loaded NLC and 137.1 ± 0.5 nm for GO-loaded NLC with narrow size distribution (PI < 0.1) in both formulations. The release profile of extract-loaded NLC formulation was slightly higher than GO-loaded NLC formulation. However, they did not follow the Higuchi model because of small amounts of γ-oryzanol loaded in NLC particles.
[Mh] Termos MeSH primário: Antioxidantes/isolamento & purificação
Portadores de Fármacos
Nanoestruturas
Oryza/química
Fenilpropionatos/isolamento & purificação
Extratos Vegetais/análise
Extratos Vegetais/isolamento & purificação
[Mh] Termos MeSH secundário: Hexanos
Ácido Linoleico/análise
Ácido Oleico/análise
Ácido Palmítico/análise
Tamanho da Partícula
Fenilpropionatos/análise
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Drug Carriers); 0 (Hexanes); 0 (Phenylpropionates); 0 (Plant Extracts); 0 (Solvents); 2UMI9U37CP (Oleic Acid); 2V16EO95H1 (Palmitic Acid); 9KJL21T0QJ (Linoleic Acid); SST9XCL51M (gamma-oryzanol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17113


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[PMID]:28480734
[Au] Autor:Kumar A; Chand G; Agnihotri VK
[Ad] Endereço:a Academy of Scientific and Innovative Research , CSIR-Institute of Himalayan Bioresource Technology , Palampur , India.
[Ti] Título:A new oxo-sterol derivative from the rhizomes of Costus speciosus.
[So] Source:Nat Prod Res;32(1):18-22, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemical investigation of the rhizomes of Costus speciosus led to the isolation of a new compound, 22-ketocholesteryl palmitate (1) along with four known compounds, 24-methylenecycloartanol (2), cycloartanol (3), stigmasterol (4) and linoleic acid (5). The structure of new compound was characterised by extensive 1D-, 2D-NMR and mass spectrometry (GC-MS and HR-ESI-MS) techniques.
[Mh] Termos MeSH primário: Costus/química
Cetosteroides/química
Rizoma/química
Esteróis/química
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Ácido Linoleico/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Espectrometria de Massas por Ionização por Electrospray/métodos
Estigmasterol/química
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (24-methylenecycloartenol); 0 (Ketosteroids); 0 (Sterols); 0 (Triterpenes); 99WUK5D0Y8 (Stigmasterol); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1324962


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[PMID]:28456993
[Au] Autor:Serna-Marquez N; Diaz-Aragon R; Reyes-Uribe E; Cortes-Reynosa P; Salazar EP
[Ad] Endereço:Departamento de Biologia Celular, Cinvestav-IPN, Av IPN # 2508, San Pedro Zacatenco, 07360, Ciudad de Mexico, Mexico.
[Ti] Título:Linoleic acid induces migration and invasion through FFAR4- and PI3K-/Akt-dependent pathway in MDA-MB-231 breast cancer cells.
[So] Source:Med Oncol;34(6):111, 2017 Jun.
[Is] ISSN:1559-131X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An increased risk of developing breast cancer has been associated with high levels of dietary fat intake. Linoleic acid (LA) is an essential fatty acid and the major ω-6 polyunsaturated fatty acid in occidental diets, which is able to induce inappropriate inflammatory responses that contribute to several chronic diseases including cancer. In breast cancer cells, LA induces migration. However, the signal transduction pathways that mediate migration and whether LA induces invasion in MDA-MB-231 breast cancer cells have not been studied in detail. We demonstrate here that LA induces Akt2 activation, invasion, an increase in NFκB-DNA binding activity, miR34a upregulation and miR9 downregulation in MDA-MB-231 cells. Moreover, Akt2 activation requires EGFR and PI3K activity, whereas migration and invasion are dependent on FFAR4, EGFR and PI3K/Akt activity. Our findings demonstrate, for the first time, that LA induces migration and invasion through an EGFR-/PI3K-/Akt-dependent pathway in MDA-MB-231 breast cancer cells.
[Mh] Termos MeSH primário: Neoplasias da Mama/metabolismo
Movimento Celular/efeitos dos fármacos
Ácido Linoleico/farmacologia
Invasividade Neoplásica/fisiopatologia
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Feminino
Seres Humanos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (O3FAR1 protein, human); 0 (Receptors, G-Protein-Coupled); 9KJL21T0QJ (Linoleic Acid); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/s12032-017-0969-3


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[PMID]:29254300
[Au] Autor:Khalid M; Bilal M; Hassani D; Iqbal HMN; Huang D
[Ad] Endereço:School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
[Ti] Título:Antimicrobial, antioxidant, cytotoxicity and LC-MS analyses of Aerva javanica: an ethnomedicinally important plant.
[So] Source:J Biol Regul Homeost Agents;31(4):963-969, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:In this study, Aerva javanica was used to extract the essential oil with notable medicinal activities. The chemical composition was investigated by high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC-MS). Ten major chemical compounds were identified as flavonoids derivatives, dihydroxylated and glycosylated metabolites. The antimicrobial, antioxidant, and cytotoxicity activities were tested using agar well-diffusion assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging and linoleic acid oxidation assays and hemolytic assay against human erythrocytes (RBCs), respectively. Plant extracts exhibited different extents of antimicrobial activities against selected bacterial and fungal strains; however, the essential oil displayed potent antimicrobial activity against all the tested strains. The percentage inhibition of linoleic acid oxidation and inhibitory concentration (IC50) were recorded to be in the range of 42.45-96.21% and 14.21-38.18 µg/mL, respectively. Cytotoxicity profile of A. javanica extracts and essential oil was found in the range of 5.82 to 14.47%. In conclusion, A. javanica essential oil could be a potential alternative to chemical additives in the food and pharmaceutical industries.
[Mh] Termos MeSH primário: Amaranthaceae/química
Anti-Infecciosos/farmacologia
Antioxidantes/farmacologia
Citotoxinas/farmacologia
Flavonoides/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Antioxidantes/isolamento & purificação
Compostos de Bifenilo/antagonistas & inibidores
Compostos de Bifenilo/química
Citotoxinas/isolamento & purificação
Flavonoides/isolamento & purificação
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Concentração Inibidora 50
Ácido Linoleico/química
Testes de Sensibilidade Microbiana
Óleos Voláteis/isolamento & purificação
Oxirredução
Picratos/antagonistas & inibidores
Picratos/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Cytotoxins); 0 (Flavonoids); 0 (Oils, Volatile); 0 (Picrates); 0 (Plant Extracts); 9KJL21T0QJ (Linoleic Acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29235833
[Au] Autor:Molodchenkova OO; Adamovskaya VG; Ciselskaya LY; Bezkrovnaya LY; Kartuzova TV; Iablonska VB
[Ti] Título:Purification and properties of lipoxygenase from wheat seedlings infected by Fusarium graminearum and treated by salicylic acid.
[So] Source:Ukr Biochem J;88(6):26-34, 2016 Nov-Dec.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Lipoxygenase from wheat seedlings in normal conditions, infected by Fusarium graminearum and treated by salicylic acid was isolated. The isolated enzyme was purified by the methods of salting-out (60% ammonium sulphate), dialysis, gel-filtration and ion-exchange chromatography. Specific activity of the purified enzyme was 8.0-12.5 ΔЕ234/mg of protein, degree of purification ­ 11.6-15.3 times. The enzyme yield was 18.3-27.9%. Molecular mass of lipoxygenase is 90 kDa, amino acid composition is distinguished by a high content of glutamic acid, proline, valine, isoleucine, leucine and low level of histidine, tyrosine, phenylalanine, threonine, tryptophan, cystein. Research of lipoxygenase substrate dependence indicated that the enzyme catalysed with the maximum velocity of the reaction of arachidonic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2, the reaction of linoleic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2 and the reaction of linolenic acid oxidation at a substrate concentration of 9.0 mM at pH 8.0. The change of wheat lipoxygenase activity depending on genotype resistance to Fusarium graminearum and millieu of germination was shown. One of the manifestations of the protective effect of salicylic acid is its ability to induce changes of lipoxygenase activity.
[Mh] Termos MeSH primário: Fungicidas Industriais/farmacologia
Lipoxigenase/isolamento & purificação
Proteínas de Plantas/isolamento & purificação
Ácido Salicílico/farmacologia
Triticum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aminoácidos/química
Ácido Araquidônico/metabolismo
Cromatografia por Troca Iônica
Resistência à Doença
Suscetibilidade a Doenças/enzimologia
Suscetibilidade a Doenças/imunologia
Fusarium/efeitos dos fármacos
Fusarium/crescimento & desenvolvimento
Fusarium/patogenicidade
Expressão Gênica
Concentração de Íons de Hidrogênio
Cinética
Ácido Linoleico/metabolismo
Lipoxigenase/metabolismo
Peso Molecular
Doenças das Plantas/imunologia
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
Proteínas de Plantas/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/enzimologia
Plântulas/imunologia
Plântulas/microbiologia
Especificidade por Substrato
Triticum/enzimologia
Triticum/imunologia
Triticum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Fungicides, Industrial); 0 (Plant Proteins); 27YG812J1I (Arachidonic Acid); 9KJL21T0QJ (Linoleic Acid); EC 1.13.11.12 (Lipoxygenase); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.06.026


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[PMID]:27770821
[Au] Autor:Zappavigna S; Scuotto M; Cossu AM; Ingrosso D; De Rosa M; Schiraldi C; Filosa R; Caraglia M
[Ad] Endereço:Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, via L. De Crecchio 7, Naples, 80138, Italy.
[Ti] Título:The 1,4 benzoquinone-featured 5-lipoxygenase inhibitor RF-Id induces apoptotic death through downregulation of IAPs in human glioblastoma cells.
[So] Source:J Exp Clin Cancer Res;35(1):167, 2016 Oct 22.
[Is] ISSN:1756-9966
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Embelin is a potent dual inhibitor of 5-lipoxigenase (5-LOX) and microsomal prostaglandin E2 synthase (mPGES)-1 that suppresses proliferation of human glioma cells and induces apoptosis by inhibiting XIAP and NF-κB signaling pathway. Synthetic structural modification yielded the derivative 3-((decahydronaphthalen-6-yl)methyl)-2,5-dihydroxycyclohexa-2,5-diene-1,4-dione (RF-Id), an embelin constrained analogue, with improved efficiency against 5-LOX in human neutrophils and anti-inflammatory activity in vivo. Taking into account that lipoxygenase (LOX) metabolites, from arachidonic acid and linoleic acid, have been implicated in tumor progression, here, we determined whether RF-Id was able to hinder glioblastoma (GBM) cancer cell growth and the related mechanisms. METHODS: U87MG and LN229 cells were plated in 96-wells and treated with increasing concentrations of RF-Id. Cell viability was evaluated by MTT assay. The effects of the compounds on cell cycle, apoptosis, oxidative stress and autophagy were assessed by flow cytometry (FACS). The mode of action was confirmed by Taqman apoptosis array and evaluating caspase cascade and NFκB pathway by western blotting technique. RESULTS: Here, we found that RF-Id induced a stronger inhibition of GBM cell growth than treatment with embelin. Flow cytometry analysis showed that RF-Id induced about 30 % apoptosis and a slight increase of autophagy after 72 h on U87-MG cells. Moreover, the compound induced an increase in the percentage of cells in G2 and S phase that was paralleled by an increase of p21 and p27 expression but no significant changes of the mitochondrial membrane potential; array analysis showed a significant upregulation of CASP8 and a downregulation of IAP family and NFκB genes in cells treated with RF-Id. RF-Id induced a significant cleavage of caspases 8, 9, 3 and 7, blocked c-IAP2/XIAP interaction by inducing XIAP degradation and inhibited NFκB pathway. CONCLUSIONS: RF-Id induced a caspase-dependent apoptosis in GBM cells by inhibiting IAP family proteins and NFκB pathway and represents a promising lead compound for designing a new class of anti-cancer drugs with multiple targets.
[Mh] Termos MeSH primário: Benzoquinonas/farmacologia
Neoplasias Encefálicas/metabolismo
Regulação para Baixo
Inibidores Enzimáticos/farmacologia
Glioblastoma/metabolismo
Proteínas Inibidoras de Apoptose/metabolismo
[Mh] Termos MeSH secundário: Araquidonato 5-Lipoxigenase/metabolismo
Ácido Araquidônico/metabolismo
Autofagia
Benzoquinonas/síntese química
Benzoquinonas/química
Neoplasias Encefálicas/tratamento farmacológico
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Glioblastoma/tratamento farmacológico
Seres Humanos
Ácido Linoleico/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (Enzyme Inhibitors); 0 (Inhibitor of Apoptosis Proteins); 27YG812J1I (Arachidonic Acid); 9KJL21T0QJ (Linoleic Acid); EC 1.13.11.34 (Arachidonate 5-Lipoxygenase); EC 1.3.11.34 (ALOX5 protein, human); SHC6U8F5ER (embelin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28965514
[Au] Autor:Turkmen S; Zamorano MJ; Fernández-Palacios H; Hernández-Cruz CM; Montero D; Robaina L; Izquierdo M
[Ad] Endereço:Aquaculture Research Group (GIA),Research Institute in Sustainable Aquaculture and Marine Conservation (IU-ECOAQUA),Universidad de Las Palmas de Gran Canaria,Crta. Taliarte s/n,35214 Telde,Spain.
[Ti] Título:Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata).
[So] Source:Br J Nutr;118(7):500-512, 2017 Oct.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and low-fishmeal (FM) diets for 1 month. The present study further investigated the effects of broodstock feeding on the same offspring when they were 16 months old and were challenged for a second time with the low-FM and low-FO diet for 2 months. The results showed that replacement of parental moderate-FO feeding with LO, combined with juvenile feeding at 4 months old with low-FM and low-FO diets, significantly (P<0·05) improved offspring growth and feed utilisation of low-FM/FO diets even when they were 16 months old: that is, when they were on the verge of their first reproductive season. Liver fatty acid composition was significantly affected by broodstock or reminder diets as well as by their interaction. Moreover, the reduction of long-chain PUFA and increase in α-linolenic acid and linoleic acid in broodstock diets lead to a significant down-regulation of hepatic lipoprotein lipase (P<0·001) and elongation of very long-chain fatty acids protein 6 (P<0·01). Besides, fatty acid desaturase 2 values were positively correlated to hepatic levels of 18 : 4n-3, 18 : 3n-6, 20 : 5n-3, 22 : 6n-3 and 22 : 5n-6. Thus, this study demonstrated the long-term nutritional programming of gilthead sea bream through broodstock feeding, the effect of feeding a 'reminder' diet during juvenile stages to improve utilisation of low-FM/FO diets and fish growth as well as the regulation of gene expression along the fish's life-cycle.
[Mh] Termos MeSH primário: Ração Animal/análise
Dieta/veterinária
Metabolismo dos Lipídeos
Dourada/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Regulação para Baixo
Ácidos Graxos Dessaturases/genética
Ácidos Graxos Dessaturases/metabolismo
Ácidos Graxos/administração & dosagem
Ácidos Graxos Insaturados/administração & dosagem
Óleos de Peixe/administração & dosagem
Ácido Linoleico/administração & dosagem
Óleo de Semente do Linho/administração & dosagem
Lipase Lipoproteica/genética
Lipase Lipoproteica/metabolismo
Fígado/metabolismo
Ácido alfa-Linolênico/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fatty Acids, Unsaturated); 0 (Fish Oils); 0RBV727H71 (alpha-Linolenic Acid); 8001-26-1 (Linseed Oil); 9KJL21T0QJ (Linoleic Acid); EC 1.14.19.- (Fatty Acid Desaturases); EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002434


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[PMID]:28945791
[Au] Autor:Zhao A; Chen F; Ning C; Wu H; Song H; Wu Y; Chen R; Zhou K; Xu X; Lu Y; Gao J
[Ad] Endereço:Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, School of Laboratory Medicine& Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
[Ti] Título:Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells.
[So] Source:PLoS One;12(9):e0185470, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we developed a rapid strategy to screen a serum-free medium for culturing the anchorage-dependent PC-3 prostate cancer cells, which was going to be prepared in large scale to generate GM-CSF/TNFα-surface-modified whole cell prostate cancer vaccine. Automated real-time cellular analysis as a rapid and non-invasive technology was used to monitor the growth of PC-3 cells in 16-well plates. At the same time, Plackett-Burman design was employed to identify the most influential formulation by integrating relevant information statistically. The effects of the 16 selected factors were evaluated during exponential cell growth and three medium constituents (EGF, FGF and linoleic acid) were identified to have significant effects on the cell growth. Subsequently, the response surface methodology with central composite design was applied to determine the interactions among the three factors so that these factors were optimized to improve cell growth. Finally, the prediction of the best combination was made under the maximal response to optimize cell growth by Design-Expert software 7.0. A total of 20 experiments were conducted to construct a quadratic model and a second-order polynomial equation. With the optimized combination validated by the stability test of serial passaging PC-3 cells, the serum-free medium had similar cell density and cell viability to the original serum medium. In summary, this high-throughput scheme minimized the screening time and may thus provide a new platform to efficiently develop the serum-free media for adherent cells.
[Mh] Termos MeSH primário: Meios de Cultura Livres de Soro/química
Neoplasias da Próstata/patologia
[Mh] Termos MeSH secundário: Vacinas Anticâncer/isolamento & purificação
Adesão Celular
Técnicas de Cultura de Células/métodos
Técnicas de Cultura de Células/estatística & dados numéricos
Linhagem Celular Tumoral
Proliferação Celular
Sobrevivência Celular
Sistemas de Computação
Fator de Crescimento Epidérmico/análise
Fatores de Crescimento de Fibroblastos/análise
Ensaios de Triagem em Larga Escala
Seres Humanos
Ácido Linoleico/análise
Masculino
Neoplasias da Próstata/imunologia
Neoplasias da Próstata/metabolismo
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Cancer Vaccines); 0 (Culture Media, Serum-Free); 62031-54-3 (Fibroblast Growth Factors); 62229-50-9 (Epidermal Growth Factor); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185470


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[PMID]:28923202
[Au] Autor:Warner DR; Liu H; Miller ME; Ramsden CE; Gao B; Feldstein AE; Schuster S; McClain CJ; Kirpich IA
[Ad] Endereço:Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky.
[Ti] Título:Dietary Linoleic Acid and Its Oxidized Metabolites Exacerbate Liver Injury Caused by Ethanol via Induction of Hepatic Proinflammatory Response in Mice.
[So] Source:Am J Pathol;187(10):2232-2245, 2017 Oct.
[Is] ISSN:1525-2191
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alcoholic liver disease is a major human health problem leading to significant morbidity and mortality in the United States and worldwide. Dietary fat plays an important role in alcoholic liver disease pathogenesis. Herein, we tested the hypothesis that a combination of ethanol and a diet rich in linoleic acid (LA) leads to the increased production of oxidized LA metabolites (OXLAMs), specifically 9- and 13-hydroxyoctadecadienoic acids (HODEs), which contribute to a hepatic proinflammatory response exacerbating liver injury. Mice were fed unsaturated (with a high LA content) or saturated fat diets (USF and SF, respectively) with or without ethanol for 10 days, followed by a single binge of ethanol. Compared to SF+ethanol, mice fed USF+ethanol had elevated plasma alanine transaminase levels, enhanced hepatic steatosis, oxidative stress, and inflammation. Plasma and liver levels of 9- and 13-HODEs were increased in response to USF+ethanol feeding. We demonstrated that primarily 9-HODE, but not 13-HODE, induced the expression of several proinflammatory cytokines in vitro in RAW264.7 macrophages. Finally, deficiency of arachidonate 15-lipoxygenase, a major enzyme involved in LA oxidation and OXLAM production, attenuated liver injury and inflammation caused by USF+ethanol feeding but had no effect on hepatic steatosis. This study demonstrates that OXLAM-mediated induction of a proinflammatory response in macrophages is one of the potential mechanisms underlying the progression from alcohol-induced steatosis to alcoholic steatohepatitis.
[Mh] Termos MeSH primário: Gorduras na Dieta/efeitos adversos
Inflamação/patologia
Ácido Linoleico/efeitos adversos
Fígado/metabolismo
Fígado/patologia
[Mh] Termos MeSH secundário: Animais
Araquidonato 15-Lipoxigenase/metabolismo
Bebedeira
Composição Corporal
Citocinas/metabolismo
Modelos Animais de Doenças
Etanol
Ácidos Linoleicos/metabolismo
Ácidos Linoleicos Conjugados/metabolismo
Macrófagos/metabolismo
Metaboloma
Camundongos
Camundongos Endogâmicos C57BL
Oxirredução
Estresse Oxidativo
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Dietary Fats); 0 (Linoleic Acids); 0 (Linoleic Acids, Conjugated); 15514-85-9 (9-hydroxy-10,12-octadecadienoic acid); 3K9958V90M (Ethanol); 5204-88-6 (13-hydroxy-9,11-octadecadienoic acid); 9KJL21T0QJ (Linoleic Acid); EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28646265
[Au] Autor:Nicolai E; Sinibaldi F; Sannino G; Laganà G; Basoli F; Licoccia S; Cozza P; Santucci R; Piro MC
[Ad] Endereço:Department of Experimental Medicine and Surgery, University of Rome 'Tor Vergata', Via Montpellier 1, 00133, Rome, Italy.
[Ti] Título:Omega-3 and Omega-6 Fatty Acids Act as Inhibitors of the Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Activity.
[So] Source:Protein J;36(4):278-285, 2017 Aug.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Polyunsaturated fatty acids have been reported to play a protective role in a wide range of diseases characterized by an increased metalloproteinases (MMPs) activity. The recent finding that omega-3 and omega-6 fatty acids exert an anti-inflammatory effect in periodontal diseases has stimulated the present study, designed to determine whether such properties derive from a direct inhibitory action of these compounds on the activity of MMPs. To this issue, we investigated the effect exerted by omega-3 and omega-6 fatty acids on the activity of MMP-2 and MMP-9, two enzymes that actively participate to the destruction of the organic matrix of dentin following demineralization operated by bacteria acids. Data obtained (both in vitro and on ex-vivo teeth) reveal that omega-3 and omega-6 fatty acids inhibit the proteolytic activity of MMP-2 and MMP-9, two enzymes present in dentin. This observation is of interest since it assigns to these compounds a key role as MMPs inhibitors, and stimulates further study to better define their therapeutic potentialities in carious decay.
[Mh] Termos MeSH primário: Ácidos Docosa-Hexaenoicos/farmacologia
Ácido Linoleico/farmacologia
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Inibidores de Metaloproteinases de Matriz/farmacologia
Ácido alfa-Linolênico/farmacologia
Ácido gama-Linolênico/farmacologia
[Mh] Termos MeSH secundário: Dente Pré-Molar/efeitos dos fármacos
Dente Pré-Molar/enzimologia
Dente Pré-Molar/ultraestrutura
Dente Canino/efeitos dos fármacos
Dente Canino/enzimologia
Dente Canino/ultraestrutura
Dentina/efeitos dos fármacos
Dentina/enzimologia
Dentina/ultraestrutura
Ensaios Enzimáticos
Expressão Gênica
Seres Humanos
Cinética
Metaloproteinase 2 da Matriz/genética
Metaloproteinase 9 da Matriz/genética
Microscopia Eletrônica de Varredura
Técnicas de Cultura de Tecidos
Extração Dentária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Matrix Metalloproteinase Inhibitors); 0RBV727H71 (alpha-Linolenic Acid); 25167-62-8 (Docosahexaenoic Acids); 78YC2MAX4O (gamma-Linolenic Acid); 9KJL21T0QJ (Linoleic Acid); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-017-9727-9



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