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[PMID]:29185745
[Au] Autor:Ning Y; Chen F; Xu X; Jin Y; Wang Z; Yang K; Jia Y
[Ad] Endereço:School of Biological Engineering, Hebei University of Science and Technology , Shijiazhuang 050018, China.
[Ti] Título:Biosynthesis of Neokestose Laurate Catalyzed by Candida antarctica Lipase B and Its Antimicrobial Activity against Food Pathogenic and Spoilage Bacteria.
[So] Source:J Agric Food Chem;65(50):11092-11099, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To increase the functionality and broaden the potential application of neokestose, neokestose laurate was biosynthesized using Candida antarctica lipase B as biocatalyst, for which a mixture of 20% DMSO in 2-methyl-2-butanol (v/v) was chosen as the reaction medium. The optimum conditions for biosynthesis were as follows: a molar ratio of vinyl laurate to neokestose of 12, a temperature of 50 °C, molecular sieves of 100 g/L, and enzyme loading of 10 g/L. Under the optimal conditions, the conversion rate was achieved over 80%. The synthesized chemical 6'-O-lauroylneokestose confirmed by nuclear magnetic resonance (NMR) exhibited good emulsification with critical micelle concentration (CMC) of 352 µM and broad antibacterial activity against Gram-positive bacteria such as Staphylococcus aureus, Listeria monocytogenes, Streptococcus mutans, Bacillus subtilis, and Bacillus cereus. Conclusively, 6'-O-lauroylneokestose was evidenced to be a dual-functional agent with emulsification and antibacterial activity, showing promising application potential in the food industry.
[Mh] Termos MeSH primário: Antibacterianos/química
Proteínas Fúngicas/química
Lauratos/química
Lipase/química
Trissacarídeos/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Biocatálise
Bactérias Gram-Positivas/efeitos dos fármacos
Lauratos/farmacologia
Trissacarídeos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fungal Proteins); 0 (Laurates); 0 (Trisaccharides); 3688-75-3 (neokestose); EC 3.1.1.3 (Lipase); EC 3.1.1.3 (lipase B, Candida antarctica)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04608


  2 / 590 MEDLINE  
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[PMID]:28671839
[Au] Autor:Ikegawa C; Ogita A; Doi T; Kumazawa F; Fujita KI; Tanaka T
[Ti] Título:Involvement of Irreversible Vacuolar Membrane Fragmentation in the Lethality of Food Emulsifier Diglycerol Monolaurate against Budding Yeast.
[So] Source:J Agric Food Chem;65(28):5650-5656, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diglycerol monolaurate (DGL) has been manufactured as a novel type of food emulsifier and is being considered for further application as a food preservative. DGL lethality was thus examined against Saccharomyces cerevisiae as a model of a yeast that causes food spoilage. In spite of its molecular structure as a nonionic surfactant, DGL could exhibit lethality at a concentration lower than that which caused disruptive damage to the yeast plasma membrane. DGL lethality was rather accompanied by a dynamic intracellular event such as a marked vacuolar membrane fragmentation. In DGL-treated cells, the tiny dots or particles of fragmented vacuolar membranes failed to fuse into the original large rounded architecture after its removal from medium, which were distinguished from those generated as a result of vacuolar fission normally accelerated under hyperosmotic conditions. Such an irreversible structural damage of the organelle membrane was considered a cause of DGL lethality.
[Mh] Termos MeSH primário: Emulsificantes/farmacologia
Membranas Intracelulares/efeitos dos fármacos
Lauratos/farmacologia
Monoglicerídeos/farmacologia
Saccharomyces cerevisiae/efeitos dos fármacos
Vacúolos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Membranas Intracelulares/metabolismo
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
Vacúolos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsifying Agents); 0 (Laurates); 0 (Monoglycerides); 27215-38-9 (monolaurin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01580


  3 / 590 MEDLINE  
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[PMID]:28494801
[Au] Autor:Aron M; Browning R; Carugo D; Sezgin E; Bernardino de la Serna J; Eggeling C; Stride E
[Ad] Endereço:Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Oxford, OX3 7DQ, UK.
[Ti] Título:Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order.
[So] Source:BMC Bioinformatics;18(1):254, 2017 May 12.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP) parameter. With the development of commercial microscopes equipped with spectral detectors, spectral imaging has become a convenient and powerful technique for measuring GP and other membrane properties. The existing tools for spectral image processing, however, are insufficient for processing the large data sets afforded by this technological advancement, and are unsuitable for processing images acquired with rapidly internalized fluorescent probes. RESULTS: Here we present a MATLAB spectral imaging toolbox with the aim of overcoming these limitations. In addition to common operations, such as the calculation of distributions of GP values, generation of pseudo-colored GP maps, and spectral analysis, a key highlight of this tool is reliable membrane segmentation for probes that are rapidly internalized. Furthermore, handling for hyperstacks, 3D reconstruction and batch processing facilitates analysis of data sets generated by time series, z-stack, and area scan microscope operations. Finally, the object size distribution is determined, which can provide insight into the mechanisms underlying changes in membrane properties and is desirable for e.g. studies involving model membranes and surfactant coated particles. Analysis is demonstrated for cell membranes, cell-derived vesicles, model membranes, and microbubbles with environmentally-sensitive probes Laurdan, carboxyl-modified Laurdan (C-Laurdan), Di-4-ANEPPDHQ, and Di-4-AN(F)EPPTEA (FE), for quantification of the local lateral density of lipids or lipid packing. CONCLUSIONS: The Spectral Imaging Toolbox is a powerful tool for the segmentation and processing of large spectral imaging datasets with a reliable method for membrane segmentation and no ability in programming required. The Spectral Imaging Toolbox can be downloaded from https://uk.mathworks.com/matlabcentral/fileexchange/62617-spectral-imaging-toolbox .
[Mh] Termos MeSH primário: Membrana Celular/química
Processamento de Imagem Assistida por Computador/métodos
Lipídeos de Membrana/química
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: 2-Naftilamina/análogos & derivados
2-Naftilamina/química
Células A549
Corantes Fluorescentes/química
Seres Humanos
Lauratos/química
Microbolhas
Microscopia Confocal
Compostos de Piridínio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Laurates); 0 (Membrane Lipids); 0 (Pyridinium Compounds); 0 (di-4-ANEPPDHQ); CKR7XL41N4 (2-Naphthylamine); Y97FBL93VW (laurdan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1656-2


  4 / 590 MEDLINE  
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[PMID]:28472615
[Au] Autor:Cyboran-Mikolajczyk S; Zylka R; Jurkiewicz P; Pruchnik H; Oszmianski J; Hof M; Kleszczynska H
[Ad] Endereço:Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland. Electronic address: sylwia.cyboran@up.wroc.pl.
[Ti] Título:Interaction of procyanidin B with membrane lipids - Fluorescence, DSC and FTIR studies.
[So] Source:Biochim Biophys Acta;1859(8):1362-1371, 2017 08.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Procyanidins, contained in many products abundant in human diet, exhibit high biological activity. However, this activity has not been fully explained at cellular and molecular levels. In this study, we determine the mechanism of interaction of procyanidin B with model lipid membrane. This mechanism was established on the basis of changes induced by B in the physical properties of lipid bilayer. The changes were investigated using steady state and time-resolved fluorescence, DSC, and FTIR. We show that procyanidin B causes changes in the arrangement of the polar heads of lipids, order of their acyl chains and the main lipid phase transition temperature. Furthermore, its presence in the membrane leads to a reduction in membrane dipole potential. Procyanidin B is anchored to membrane via hydrogen bonds formed between its OH groups and the PO and CO groups of lipids, causing changes in both hydrophilic and hydrophobic regions of the membrane.
[Mh] Termos MeSH primário: 2-Naftilamina/análogos & derivados
Biflavonoides/química
Catequina/química
Di-Hidropiridinas/química
Dimiristoilfosfatidilcolina/química
Lauratos/química
Bicamadas Lipídicas/química
Proantocianidinas/química
[Mh] Termos MeSH secundário: 2-Naftilamina/química
Varredura Diferencial de Calorimetria
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Transição de Fase
Espectrometria de Fluorescência
Espectroscopia de Infravermelho com Transformada de Fourier
Temperatura Ambiente
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylic acid monomethyl ester); 0 (Biflavonoids); 0 (Dihydropyridines); 0 (Laurates); 0 (Lipid Bilayers); 0 (Proanthocyanidins); 2TC1A0KEAQ (procyanidin B3); 8R1V1STN48 (Catechin); CKR7XL41N4 (2-Naphthylamine); U86ZGC74V5 (Dimyristoylphosphatidylcholine); Y97FBL93VW (laurdan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


  5 / 590 MEDLINE  
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[PMID]:28302764
[Au] Autor:Lohrke J; Siebeneicher H; Berger M; Reinhardt M; Berndt M; Mueller A; Zerna M; Koglin N; Oden F; Bauser M; Friebe M; Dinkelborg LM; Huetter J; Stephens AW
[Ad] Endereço:Bayer AG, Drug Discovery, Berlin, Germany; and.
[Ti] Título:F-GP1, a Novel PET Tracer Designed for High-Sensitivity, Low-Background Detection of Thrombi.
[So] Source:J Nucl Med;58(7):1094-1099, 2017 Jul.
[Is] ISSN:1535-5667
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thromboembolic diseases such as myocardial infarction, stroke, transient ischemic attacks, and pulmonary embolism are major causes of morbidity and mortality worldwide. Glycoprotein IIb/IIIa (GPIIb/IIIa) is the key receptor involved in platelet aggregation and is a validated target for therapeutic approaches and diagnostic imaging. The aim of this study was to develop and characterize a specific small-molecule tracer for PET imaging that binds with high affinity to GPIIb/IIIa receptors and has suitable pharmacokinetic properties to overcome limitations of previous approaches. Binding of F-GP1 to GPIIb/IIIa receptors was investigated in competition binding assays and autoradiography using a fresh cardiac thrombus from an explanted human heart. The clot-to-blood ratio for F-GP1 was investigated by an in vitro blood flow model. Biodistribution and thrombus detection was investigated in cynomolgus monkeys after insertion of a roughened catheter into either the vena cava or the aorta. F-GP1 is an F-labeled small molecule for PET imaging of thrombi. The half maximal inhibitory concentration of F-GP1 to GPIIb/IIIa was 20 nM. F-GP1 bound to thrombi with a mean clot-to-blood ratio of 95. Binding was specific and can be displaced by excess nonradioactive derivative. Binding was not affected by anticoagulants such as aspirin or heparin. F-GP1 showed rapid blood clearance and a low background after intravenous injection in cynomolgus monkeys. Small arterial, venous thrombi, thrombotic depositions on damaged endothelial surface, and small cerebral emboli were detected in vivo by PET imaging. F-GP1 binds specifically with high affinity to the GPIIb/IIIa receptor involved in platelet aggregation. Because of its favorable preclinical characteristics, F-GP1 is currently being investigated in a human clinical study.
[Mh] Termos MeSH primário: Glutamina/análogos & derivados
Lauratos/farmacocinética
Imagem Molecular/métodos
Complexo Glicoproteico GPIIb-IIIa de Plaquetas/metabolismo
Tomografia por Emissão de Pósitrons/métodos
Trombose/diagnóstico por imagem
Trombose/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Radioisótopos de Flúor/química
Radioisótopos de Flúor/farmacocinética
Glutamina/farmacocinética
Seres Humanos
Marcação por Isótopo/métodos
Macaca fascicularis
Compostos Radiofarmacêuticos/síntese química
Compostos Radiofarmacêuticos/farmacocinética
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorine Radioisotopes); 0 (Laurates); 0 (Platelet Glycoprotein GPIIb-IIIa Complex); 0 (Radiopharmaceuticals); 0 (dibutyllauroylglutamide); 0RH81L854J (Glutamine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.2967/jnumed.116.188896


  6 / 590 MEDLINE  
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[PMID]:28126480
[Au] Autor:Brewer J; Thoke HS; Stock RP; Bagatolli LA
[Ad] Endereço:MEMPHYS - Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark. Electronic address: brewer@memphys.sdu.dk.
[Ti] Título:Enzymatic studies on planar supported membranes using a widefield fluorescence LAURDAN Generalized Polarization imaging approach.
[So] Source:Biochim Biophys Acta;1859(5):888-895, 2017 05.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We introduce a custom-built instrument designed to perform fast LAURDAN Generalized Polarization (GP) imaging on planar supported membranes. It is mounted on a widefield fluorescence microscope and allows kinetic analysis of the GP function in the millisecond time scale, largely improving the temporal resolution previously achieved using laser scanning based microscopes. A dedicated protocol to calibrate LAURDAN GP data obtained with charge-coupled device (CCD) cameras as detectors is also presented, enabling reliable assignment of GP values in the field of view. Using this methodology we studied structural and dynamical transformations induced by Sphingomyelinase D (SM-D) on planar supported membranes composed of N-lauroyl sphingomyelin (C SM). GP data show the evolution of an initially compositionally homogeneous symmetric bilayer existing in a single liquid disordered phase, to an intermediate configuration showing coexistence of liquid disordered and solid ordered domains, which are not always in-register across the axial plane of the bilayer. This intermediate state, caused by the transformation of C SM to C -ceramide-1-phosphate in the distal leaflet of the bilayer, evolved to a single solid ordered phase at longer time scales. Additionally, we comparatively studied this system using the membrane fluorophore DiIC The advantages and limitations of both fluorescent dyes are discussed, emphasizing the adequacy of LAURDAN GP imaging to explore this type of membrane phenomena.
[Mh] Termos MeSH primário: 2-Naftilamina/análogos & derivados
Polarização de Fluorescência
Corantes Fluorescentes
Lauratos
Bicamadas Lipídicas/química
Diester Fosfórico Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Imagem Óptica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Laurates); 0 (Lipid Bilayers); CKR7XL41N4 (2-Naphthylamine); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.41 (sphingomyelin phosphodiesterase D); Y97FBL93VW (laurdan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


  7 / 590 MEDLINE  
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[PMID]:28112032
[Au] Autor:Zhang Y; Yin J; Zhang L; Qi CC; Ma ZL; Gao LP; Wang DG; Jing YH
[Ad] Endereço:a Department of Neurology , People Hospital of Gansu Province , Lanzhou , P.R. China.
[Ti] Título:Spermidine preconditioning ameliorates laurate-induced brain injury by maintaining mitochondrial stability.
[So] Source:Neurol Res;39(3):248-258, 2017 Mar.
[Is] ISSN:1743-1328
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ischemic precondition plays a protective effect during cerebral ischemia. This effect partly depends on the autophagic activity. However, whether the activity of autophagy can exert the protective effects after cerebral ischemia is unclear. In this study, rats were treated with spermidine, an activator of autophagy, and injected with sodium laurate via the internal carotid artery to stimulate cerebral small vessel disease (CSVD). The effects of the spermidine precondition on brain injury were evaluated by behavioural test, histology assay, ultrastructure observation, and autophagic-related signals. Furthermore, the mitochondria of brain tissue were isolated, and mitDNA were extracted. The stability of mitDNA was analyzed by quantitative real-time PCR. Results showed that the penetrating artery of the striatum was damaged. This damage was accompanied by neural inflammation characterized by an increase in Fluoro-Jade C (FJC)-positive cells after sodium laurate injection. Spermidine pretreatment decreased the deletion of mitDNA and the autophagy hyperactivity induced by the laurate injection. Likewise, spermidine reduced the neurological deficit and FJC reactivation of striatum at 48 h after laurate injection. These results suggested sodium laurate injection through the internal carotid artery can induce the pathological features of CSVD characterized by the damage of penetrating artery, neurological deficit, mitochondrial impairment, and autophagic hyperactivity. Pretreatment with spermidine can ameliorate these outcomes. Further study indicated that the protective effect of the spermidine precondition is associated with the maintenance of mitochondrial stability and proper autophagy activity.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Doenças de Pequenos Vasos Cerebrais/prevenção & controle
Corpo Estriado/efeitos dos fármacos
DNA Mitocondrial/efeitos dos fármacos
Espermidina/farmacologia
[Mh] Termos MeSH secundário: Animais
Doenças de Pequenos Vasos Cerebrais/induzido quimicamente
Modelos Animais de Doenças
Lauratos/farmacologia
Masculino
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Laurates); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1080/01616412.2017.1283830


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[PMID]:28110048
[Au] Autor:Lopes LQ; Santos CG; de Almeida Vaucher R; Raffin RP; da Silva AS; Baretta D; Maccari AP; Giombelli LC; Volpato A; Arruda J; de Ávila Scheeren C; Baldisserotto B; Santos RC
[Ad] Endereço:Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post-Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil. Electronic address: leonardoquintanalopes@gmail.com.
[Ti] Título:Ecotoxicology of Glycerol Monolaurate nanocapsules.
[So] Source:Ecotoxicol Environ Saf;139:73-77, 2017 May.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Glycerol Monolaurate (GML) is a compound with known antimicrobial potential, however it is not much used due to its low solubility in water and high melting point. The nanoencapsulation of some drugs offers several advantages such as improved stability and solubility in water. The present study aimed to produce, characterize, and evaluate the ecotoxicity of GML nanocapsules. The nanocapsules were produced and presented a mean diameter of 210nm, polydispersity index of 0.044, and zeta potential of -23.4mV. The electron microscopy images showed the nanometric size and spherical shape. The assay in soil showed that GML has a high toxicity while the GML nanocapsules showed decreased toxic effects. Nanostructuration also protected the Rhamdia quelen against the toxic effects of GML. Concluding, the formulation shows positive results and is useful to predict the success of development besides not damaging the soil.
[Mh] Termos MeSH primário: Anti-Infecciosos
Artrópodes/crescimento & desenvolvimento
Peixes/crescimento & desenvolvimento
Lauratos/toxicidade
Monoglicerídeos/toxicidade
Nanocápsulas/toxicidade
Poluentes do Solo/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/administração & dosagem
Anti-Infecciosos/toxicidade
Artrópodes/efeitos dos fármacos
Ecotoxicologia
Exposição Ambiental
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Laurates); 0 (Monoglycerides); 0 (Nanocapsules); 0 (Soil Pollutants); 0 (Water Pollutants, Chemical); 27215-38-9 (monolaurin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


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[PMID]:27987460
[Au] Autor:Tanaka H; Oasa S; Kinjo M; Tange K; Nakai Y; Harashima H; Akita H
[Ad] Endereço:Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Sapporo City, Hokkaido 060-0812, Japan.
[Ti] Título:Temperature and pH sensitivity of a stabilized self-nanoemulsion formed using an ionizable lipid-like material via an oil-to-surfactant transition.
[So] Source:Colloids Surf B Biointerfaces;151:95-101, 2017 Mar 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lipids functionalized with tertiary amines (ionizable lipids) for a pH-dependent positive charge have been developed extensively as a carrier material for delivering nucleic acids. We previously developed an SS-cleavable proton-activated lipid-like material (ssPalm) as a component of a functionalized lipid envelope structure of a nanoparticle that encapsulated plasmid DNA and short interfering RNA. In this study, we report on the unique characteristics of such an ionizable lipid: the formation of a nano-sized emulsion (ave. 40nm) via pH-triggered self-emulsification in the absence of a cargo (nucleic acids). The particle has a neutral charge at physiological pH and is stabilized by helper lipids and polyethyleneglycol (PEG)-conjugated lipids. The generalized polarization of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan), which indicates the surface polarity caused by the invasion of water onto the surface, changes dynamically in response to pH and temperature, while the fluidity of the intra-particle compartment, as measured by the fluorescence anisotropy of 1,6-Diphenyl-1,3,5-hexatriene (DPH), is not affected. Even when the particle contains a high density of PEG on the surface, it shows a high fusogenecity to negatively charged liposomes in response to an acidic pH to a higher degree than a conventional cationic lipid. These characteristics suggest that the ssPalm particle possesses unique properties for delivering lipophilic drugs across the biomembrane.
[Mh] Termos MeSH primário: 2-Naftilamina/análogos & derivados
Lauratos/química
Lipídeos/química
Lipossomos/química
Tensoativos/química
[Mh] Termos MeSH secundário: 2-Naftilamina/química
Anisotropia
Difenilexatrieno/química
Emulsões
Etanol/química
Concentração de Íons de Hidrogênio
Nanopartículas/química
Ácidos Nucleicos/química
Óleos
Tamanho da Partícula
Plasmídeos/metabolismo
Polietilenoglicóis/química
RNA Interferente Pequeno/metabolismo
Solubilidade
Propriedades de Superfície
Temperatura Ambiente
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Laurates); 0 (Lipids); 0 (Liposomes); 0 (Nucleic Acids); 0 (Oils); 0 (RNA, Small Interfering); 0 (Surface-Active Agents); 059QF0KO0R (Water); 1720-32-7 (Diphenylhexatriene); 30IQX730WE (Polyethylene Glycols); 3K9958V90M (Ethanol); CKR7XL41N4 (2-Naphthylamine); Y97FBL93VW (laurdan)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27966664
[Au] Autor:Wei Y; Shi Q; Wang Z; Zhang R; Su L; Quamruzzaman Q; Rahman M; Chen F; Christiani DC
[Ad] Endereço:Department of Biostatistics, Ministry of Education Key Laboratory for Modern Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.
[Ti] Título:Maternal/fetal metabolomes appear to mediate the impact of arsenic exposure on birth weight: A pilot study.
[So] Source:J Expo Sci Environ Epidemiol;27(3):313-319, 2017 May.
[Is] ISSN:1559-064X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arsenic exposure has been associated with low birth weight. However, the underlying mechanisms are not well understood. Alterations to metabolites may act as causal mediators of the effect of arsenic exposure on low birth weight. This pilot study aimed to explore the role of metabolites in mediating the association of arsenic exposure on infant birth weight. Study samples were selected from a well-established prospectively enrolled cohort in Bangladesh comprising 35 newborns and a subset of 20 matched mothers. Metabolomics profiling was performed on 35 cord blood samples and 20 maternal peripheral blood samples collected during the second trimester of pregnancy. Inorganic arsenic (iAs) exposure was evaluated via cord blood samples and maternal toenail samples collected during the first trimester. Multiple linear regression and mediation analyses were used to explore the relationship between iAs exposure, metabolite alterations, and low birth weight. Cord blood arsenic level was correlated with elevated levels of 17-methylstearate, laurate (12:0) and 4-vinylphenol sulfate along with lower birth weight. Prenatal maternal toenail iAs level was associated with two peripheral blood metabolites (butyrylqlycine and tartarate), which likely contributed to higher cord blood iAs levels both independently and interactively. Findings of this pilot study indicate that both intrauterine and maternal peripheral blood metabolites appear to influence the toxic effect of inorganic arsenic exposure on low birth weight.
[Mh] Termos MeSH primário: Arsênico/efeitos adversos
Arsênico/metabolismo
Biomarcadores/sangue
Exposição Ambiental/efeitos adversos
Recém-Nascido de Baixo Peso
Exposição Materna/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Bangladesh
Peso ao Nascer
Exposição Ambiental/análise
Feminino
Sangue Fetal/química
Seres Humanos
Recém-Nascido
Lauratos
Modelos Lineares
Masculino
Metaboloma
Unhas/química
Fenóis/sangue
Projetos Piloto
Gravidez
Primeiro Trimestre da Gravidez
Estudos Prospectivos
Inquéritos e Questionários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Laurates); 0 (Phenols); N712M78A8G (Arsenic); OA7V1SM8YL (4-vinylphenol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE
[do] DOI:10.1038/jes.2016.74



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