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[PMID]:28462811
[Au] Autor:Iqbal J; Walsh MT; Hammad SM; Hussain MM
[Ad] Endereço:Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, New York, NY 11203, USA; King Abdullah International Medical Research Center, MNGHA, Al Ahsa 31982, Saudi Arabia.
[Ti] Título:Sphingolipids and Lipoproteins in Health and Metabolic Disorders.
[So] Source:Trends Endocrinol Metab;28(7):506-518, 2017 07.
[Is] ISSN:1879-3061
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sphingolipids are structurally and functionally diverse molecules with significant physiologic functions and are found associated with cellular membranes and plasma lipoproteins. The cellular and plasma concentrations of sphingolipids are altered in several metabolic disorders and may serve as prognostic and diagnostic markers. Here we discuss various sphingolipid transport mechanisms and highlight how changes in cellular and plasma sphingolipid levels contribute to cardiovascular disease, obesity, diabetes, insulin resistance, and nonalcoholic fatty liver disease (NAFLD). Understanding of the mechanisms involved in intracellular transport, secretion, and extracellular transport may provide novel information that might be amenable to therapeutic targeting for the treatment of various metabolic disorders.
[Mh] Termos MeSH primário: Saúde
Lipoproteínas/fisiologia
Doenças Metabólicas/etiologia
Esfingolipídeos/metabolismo
Esfingolipídeos/fisiologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Resistência à Insulina/fisiologia
Lipoproteínas/sangue
Lipoproteínas/metabolismo
Doenças Metabólicas/sangue
Doenças Metabólicas/metabolismo
Esfingolipídeos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Lipoproteins); 0 (Sphingolipids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29340525
[Au] Autor:França CN; Mendes CC; Ferreira CES
[Ad] Endereço:Pós Graduação em Ciências da Saúde, Universidade Santo Amaro, São Paulo, SP, Brasil.
[Ti] Título:Time collection and storage conditions of lipid profile.
[So] Source:Braz J Med Biol Res;51(3):e6955, 2018 Jan 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
[Mh] Termos MeSH primário: Coleta de Amostras Sanguíneas/métodos
Lipídeos/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Análise Química do Sangue
Preservação de Sangue
Coleta de Amostras Sanguíneas/instrumentação
Coleta de Amostras Sanguíneas/normas
Colesterol/sangue
Feminino
Seres Humanos
Laboratórios/normas
Lipoproteínas/sangue
Masculino
Meia-Idade
Estudos Prospectivos
Temperatura Ambiente
Fatores de Tempo
Triglicerídeos/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (Lipoproteins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:28449094
[Au] Autor:Ehrhardt N; Doche ME; Chen S; Mao HZ; Walsh MT; Bedoya C; Guindi M; Xiong W; Ignatius Irudayam J; Iqbal J; Fuchs S; French SW; Mahmood Hussain M; Arditi M; Arumugaswami V; Péterfy M
[Ad] Endereço:Department of Basic Medical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA.
[Ti] Título:Hepatic Tm6sf2 overexpression affects cellular ApoB-trafficking, plasma lipid levels, hepatic steatosis and atherosclerosis.
[So] Source:Hum Mol Genet;26(14):2719-2731, 2017 07 15.
[Is] ISSN:1460-2083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The human transmembrane 6 superfamily member 2 (TM6SF2) gene has been implicated in plasma lipoprotein metabolism, alcoholic and non-alcoholic fatty liver disease and myocardial infarction in multiple genome-wide association studies. To investigate the role of Tm6sf2 in metabolic homeostasis, we generated mice with elevated expression using adeno-associated virus (AAV)-mediated gene delivery. Hepatic overexpression of mouse Tm6sf2 resulted in phenotypes previously observed in Tm6sf2-deficient mice including reduced plasma lipid levels, diminished hepatic triglycerides secretion and increased hepatosteatosis. Furthermore, increased hepatic Tm6sf2 expression protected against the development of atherosclerosis in LDL-receptor/ApoB48-deficient mice. In cultured human hepatocytes, Tm6sf2 overexpression reduced apolipoprotein B secretion and resulted in its accumulation within the endoplasmic reticulum (ER) suggesting impaired ER-to-Golgi trafficking of pre-very low-density lipoprotein (VLDL) particles. Analysis of two metabolic trait-associated coding polymorphisms in the human TM6SF2 gene (rs58542926 and rs187429064) revealed that both variants impact TM6SF2 expression by affecting the rate of protein turnover. These data demonstrate that rs58542926 (E167K) and rs187429064 (L156P) are functional variants and suggest that they influence metabolic traits through altered TM6SF2 protein stability. Taken together, our results indicate that cellular Tm6sf2 level is an important determinant of VLDL metabolism and further implicate TM6SF2 as a causative gene underlying metabolic disease and trait associations at the 19p13.11 locus.
[Mh] Termos MeSH primário: Apolipoproteínas B/metabolismo
Aterosclerose/metabolismo
Fígado/metabolismo
Proteínas de Membrana/biossíntese
Hepatopatia Gordurosa não Alcoólica/metabolismo
[Mh] Termos MeSH secundário: Animais
Apolipoproteínas B/genética
Aterosclerose/sangue
Aterosclerose/genética
Células Cultivadas
Retículo Endoplasmático/metabolismo
Feminino
Estudo de Associação Genômica Ampla
Complexo de Golgi/metabolismo
Células Hep G2
Hepatócitos/metabolismo
Seres Humanos
Lipoproteínas/sangue
Masculino
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Hepatopatia Gordurosa não Alcoólica/sangue
Hepatopatia Gordurosa não Alcoólica/genética
Polimorfismo de Nucleotídeo Único
Transporte Proteico
Triglicerídeos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Apolipoproteins B); 0 (Lipoproteins); 0 (Membrane Proteins); 0 (TM6SF2 protein, human); 0 (Triglycerides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180225
[Lr] Data última revisão:
180225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/hmg/ddx159


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[PMID]:29304079
[Au] Autor:Okada T; Ohama T; Okazaki M; Kanno K; Matsuda H; Sairyo M; Zhu Y; Saga A; Kobayashi T; Masuda D; Koseki M; Nishida M; Sakata Y; Yamashita S
[Ad] Endereço:Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
[Ti] Título:Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency.
[So] Source:PLoS One;13(1):e0190875, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: We previously reported that patients with cholesteryl ester transfer protein (CETP) deficiency (CETP-D) have a higher prevalence of atherosclerotic cardiovascular disease, in spite of increased HDL-C levels. However, characterization of HDL in CETP-D has not been well described. Therefore, we examined HDL particle number (PN) rather than HDL-C level. APPROACH AND RESULTS: Nine patients with CETP-D and 9 normolipidemic subjects were enrolled. We performed gel permeation high-performance liquid chromatography (GP-HPLC) analysis, determined the cholesterol and triglyceride composition of all lipoprotein subclasses, and calculated the PN of each subclass, which consisted of 3 VLDL (large, medium, and small), 4 LDL (large, medium, small, and very small), and 5 HDL (very large, large, medium, small, and very small) subclasses. The PNs of large and medium LDL were significantly lower in CETP-D than that in healthy subjects (0.66- and 0.63-fold decrease, respectively; p<0.001), whereas the PN of very small LDL, which is known to be atherogenic, was significantly higher (1.36-fold increase, p = 0.016). The PNs of very large and large HDL in CETP-D were markedly higher than that in healthy subjects (19.9- and 4.5-fold increase, respectively; p<0.001), whereas the PNs of small and very small HDL, which have more potent anti-atherogenic functions, were significantly lower (0.76- and 0.61-fold decrease, respectively; p<0.001). CONCLUSION: We have assessed the PNs of detailed subclasses of patients with CETP-D for the first time. The PN of larger HDL was markedly increased, that of smaller HDL was decreased, and that of very small LDL was increased, suggesting that CETP-D has pro-atherogenic lipoprotein properties.
[Mh] Termos MeSH primário: Proteínas de Transferência de Ésteres de Colesterol/deficiência
Cromatografia em Gel/métodos
Cromatografia Líquida de Alta Pressão/métodos
Erros Inatos do Metabolismo Lipídico/sangue
Lipoproteínas/classificação
[Mh] Termos MeSH secundário: Adulto
Proteínas de Transferência de Ésteres de Colesterol/sangue
Feminino
Seres Humanos
Lipoproteínas/sangue
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholesterol Ester Transfer Proteins); 0 (Lipoproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190875


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[PMID]:28463110
[Au] Autor:Gong B; Shen W; Xiao W; Meng Y; Meng A; Jia S
[Ad] Endereço:State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
[Ti] Título:The Sec14-like phosphatidylinositol transfer proteins Sec14l3/SEC14L2 act as GTPase proteins to mediate Wnt/Ca signaling.
[So] Source:Elife;6, 2017 05 02.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The non-canonical Wnt/Ca signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that the zebrafish/human phosphatidylinositol transfer protein Sec14l3/SEC14L2 act as GTPase proteins to transduce Wnt signals from Frizzled to phospholipase C (PLC). Depletion of attenuates Wnt/Ca responsive activity and causes convergent and extension (CE) defects in zebrafish embryos. Biochemical analyses in mammalian cells indicate that Sec14l3-GDP forms complex with Frizzled and Dishevelled; Wnt ligand binding of Frizzled induces translocation of Sec14l3 to the plasma membrane; and then Sec14l3-GTP binds to and activates phospholipase Cδ4a (Plcδ4a); subsequently, Plcδ4a initiates phosphatidylinositol-4,5-bisphosphate (PIP ) signaling, ultimately stimulating calcium release. Furthermore, Plcδ4a can act as a GTPase-activating protein to accelerate the hydrolysis of Sec14l3-bound GTP to GDP. Our data provide a new insight into GTPase protein-coupled Wnt/Ca signaling transduction.
[Mh] Termos MeSH primário: Sinalização do Cálcio
Proteínas de Transporte/metabolismo
GTP Fosfo-Hidrolases/metabolismo
Lipoproteínas/metabolismo
Proteínas de Transferência de Fosfolipídeos/metabolismo
Transativadores/metabolismo
Via de Sinalização Wnt
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Receptores Frizzled/metabolismo
Seres Humanos
Fosfolipases Tipo C/metabolismo
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Frizzled Receptors); 0 (Lipoproteins); 0 (Phospholipid Transfer Proteins); 0 (SEC14L2 protein, human); 0 (SEC14L3 protein, human); 0 (Trans-Activators); EC 3.1.4.- (Type C Phospholipases); EC 3.6.1.- (GTP Phosphohydrolases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180211
[Lr] Data última revisão:
180211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29244927
[Au] Autor:Tinkov AA; Gatiatulina ER; Popova EV; Polyakova VS; Skalvaya AA; Agletdinov EF; Nikonorov AA; Radysh IV; Kkarganov MY; Skalny AV
[Ti] Título:The impact of adipogenic diet on rats' tissue trace elements content.
[So] Source:Patol Fiziol Eksp Ter;60(4):79-85, 2016 Oct-Dec.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The purpose: The influence of high-fat diet (HFD) on trace elements status, adipokine level, and markers of carbohydrate and lipid metabolism in weanling Wistar rats was investigated. Methods: A total of 20 male 1-months-old Wistar rats divided into two equal groups were used in the present study. The first group of animals obtained a standard diet (STD), whereas animals from the second group (NAFLD) were maintained on high-fat diet containing 10 and 31.6% of total calories from fat, respectively, during 1 month. Fat diet (HFD). Trace element status (using inductively coupled plasma mass spectrometry), serum levels of insulin, adiponectin, and leptin (using enzyme-linked immunosorbent assay), total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), glucose (spectrophotometrically), apolipoprotein A1 (ApoA1) and B (ApoB) (using immunoturbidimetric method) were assessed. Results: It was shown that 1-month HFD feeding resulted in significant increase of EDAT, RPAT, total adipose tissue mass, and adipocyte area. HFD-fed animals were also characterized by a significant increase in circulating leptin levels and leptin-to-adiponectin ratio as compared to the control ones. No significant HFD-related difference in serum lipid spectrum, adiponectin, apolipoproteins, glucose, insulin, and HOMA-IR were revealed. Liver Cu, I, Mn, Se, Zn; EDAT Cr, V, Co, Cu, Fe,I, and RPAT Co, Cu, I, Cr, V, Fe, and Zn were significantly decreased in HFD-fed rats in comparison with the control group levels. Hair Co, Mn, Si, and V levels significantly exceeded the respective control values, whereas Se and I content were decreased in studied animals. At the same time, only serum Cu was significantly decreased in HFD-fed rats. Conclusion: The interplay between the impaired trace elements metabolism of HFD-fed weanling Wistar rats and disorder of adipokine balance was demonstrated. It is supposed that the altered trace elements status is primary and precedes other metabolic obesity-related disturbances.
[Mh] Termos MeSH primário: Gorduras na Dieta/farmacologia
Lipídeos/sangue
Lipoproteínas/sangue
Oligoelementos/sangue
[Mh] Termos MeSH secundário: Animais
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Lipids); 0 (Lipoproteins); 0 (Trace Elements)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


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[PMID]:29176657
[Au] Autor:Agrawal S; Zaritsky JJ; Fornoni A; Smoyer WE
[Ad] Endereço:Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, and Department of Pediatrics, The Ohio State University, 700 Children's Drive, W303, Columbus, Ohio 43205, USA.
[Ti] Título:Dyslipidaemia in nephrotic syndrome: mechanisms and treatment.
[So] Source:Nat Rev Nephrol;14(1):57-70, 2018 Jan.
[Is] ISSN:1759-507X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nephrotic syndrome is a highly prevalent disease that is associated with high morbidity despite notable advances in its treatment. Many of the complications of nephrotic syndrome, including the increased risk of atherosclerosis and thromboembolism, can be linked to dysregulated lipid metabolism and dyslipidaemia. These abnormalities include elevated plasma levels of cholesterol, triglycerides and the apolipoprotein B-containing lipoproteins VLDL and IDL; decreased lipoprotein lipase activity in the endothelium, muscle and adipose tissues; decreased hepatic lipase activity; and increased levels of the enzyme PCSK9. In addition, there is an increase in the plasma levels of immature HDL particles and reduced cholesterol efflux. Studies from the past few years have markedly improved our understanding of the molecular pathogenesis of nephrotic syndrome-associated dyslipidaemia, and also heightened our awareness of the associated exacerbated risks of cardiovascular complications, progressive kidney disease and thromboembolism. Despite the absence of clear guidelines regarding treatment, various strategies are being increasingly utilized, including statins, bile acid sequestrants, fibrates, nicotinic acid and ezetimibe, as well as lipid apheresis, which seem to also induce partial or complete clinical remission of nephrotic syndrome in a substantial percentage of patients. Future potential treatments will likely also include inhibition of PCSK9 using recently-developed anti-PCSK9 monoclonal antibodies and small inhibitory RNAs, as well as targeting newly identified molecular regulators of lipid metabolism that are dysregulated in nephrotic syndrome.
[Mh] Termos MeSH primário: Anticolesterolemiantes/uso terapêutico
Dislipidemias/tratamento farmacológico
Ácidos Fíbricos/uso terapêutico
Inibidores de Hidroximetilglutaril-CoA Redutases/uso terapêutico
Síndrome Nefrótica/metabolismo
[Mh] Termos MeSH secundário: Colesterol/metabolismo
HDL-Colesterol/metabolismo
VLDL-Colesterol/metabolismo
Dislipidemias/complicações
Dislipidemias/metabolismo
Ezetimiba/uso terapêutico
Seres Humanos
Hipolipemiantes/uso terapêutico
Lipase/metabolismo
Lipase Lipoproteica/metabolismo
Lipoproteínas/metabolismo
Síndrome Nefrótica/complicações
Niacina/uso terapêutico
Pró-Proteína Convertase 9/metabolismo
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anticholesteremic Agents); 0 (Cholesterol, HDL); 0 (Cholesterol, VLDL); 0 (Fibric Acids); 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors); 0 (Hypolipidemic Agents); 0 (Lipoproteins); 0 (Triglycerides); 0 (lipoprotein cholesterol); 2679MF687A (Niacin); 97C5T2UQ7J (Cholesterol); EC 3.1.1.3 (Lipase); EC 3.1.1.3 (hepatic lipase, human); EC 3.1.1.34 (Lipoprotein Lipase); EC 3.4.21.- (PCSK9 protein, human); EC 3.4.21.- (Proprotein Convertase 9); EOR26LQQ24 (Ezetimibe)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1038/nrneph.2017.155


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[PMID]:27776640
[Au] Autor:Moreno-Gordaliza E; van der Lee SJ; Demirkan A; van Duijn CM; Kuiper J; Lindenburg PW; Hankemeier T
[Ad] Endereço:Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Faculty of Science, Universiteit Leiden, Einsteinweg 55, 2300 RA Leiden, The Netherlands. Electronic address: emorenog@ucm.es.
[Ti] Título:A novel method for serum lipoprotein profiling using high performance capillary isotachophoresis.
[So] Source:Anal Chim Acta;944:57-69, 2016 Nov 09.
[Is] ISSN:1873-4324
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new capillary isotachophoresis (cITP) method for lipoprotein profiling with superior lipoprotein coverage compared to previous methods has been developed, resolving twice as many lipoprotein species (18 peaks/fractions) in serum or plasma in less than 9.5 min. For this, a novel mixture of 24 spacers, including amino acids, dipeptides and sulfonic acids, was developed and fine-tuned, using predictive software (PeakMaster) and testing of spiked serum samples. Lipoprotein peaks were identified by serum-spiking with reference lipoproteins. Compatibility with common lipophilic stains for selective lipoprotein detection with either UV/Vis or laser-induced fluorescence was demonstrated. A special new capillary with a neutral coating (combining water-compatible OV1701-OH deactivation and methylation) was used for the first time for electrodriven separations, allowing very stable separations in a pH 8.8-9.4 gradient system, being functional for more than 100 injections. Excellent reproducibility was achieved, with coefficients of variation lower than 2.6% for absolute migration times. Comparison was performed with human plasma samples analyzed by NMR, leading to similar results with cITP after multivariate statistics, regarding group-clustering and lipoprotein species correlation. The new cITP method was applied to the analysis of serum samples from a LDL receptor knock-out mice model fed either a normal diet or a western-type diet. Differences in the lipoprotein levels and in the sublipoprotein types were detected, showing a shift to more atherogenic particles due to the high cholesterol diet. In summary, this novel method will allow more detailed and informative profiling of lipoprotein particle subtypes for cardiovascular disease research.
[Mh] Termos MeSH primário: Análise Química do Sangue/métodos
Eletroforese Capilar/métodos
Isotacoforese/métodos
Lipoproteínas/sangue
Lipoproteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Inativação de Genes
Seres Humanos
Camundongos
Receptores de LDL/deficiência
Receptores de LDL/genética
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipoproteins); 0 (Receptors, LDL)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:29284249
[Au] Autor:Schreterova E; Bhide M; Potocnakova L; Borszekova Pulzova L
[Ad] Endereço:Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, Slovakia. eva.kendrovska@gmail.com.
[Ti] Título:Design, construction and evaluation of multi-epitope antigens for diagnosis of Lyme disease.
[So] Source:Ann Agric Environ Med;24(4):696-701, 2017 Dec 23.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Introduction and objective. Lyme disease (LD) is the most common vector-borne disease in the temperate zone of the Northern Hemisphere. Diagnosis of LD is mainly based on clinical symptoms supported with serology (detection of anti-Borrelia antibodies) and is often misdiagnosed in areas of endemicity. MATERIAL AND METHODS: In this study, the chimeric proteins (A/C-2, A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA and OspC from Borrelia genospecies prevalent in Eastern Slovakia, were designed, over-expressed in E. coli, and used to detect specific anti-Borrelia antibodies in serologically characterized sera from patients with Lyme-like symptoms to evaluate their diagnostic potential. RESULTS: Results showed that chimeras vary in their immuno-reactivity when tested with human sera. Compared with the results obtained from a two-tier test, the application of recombinant multi-epitope chimeric proteins as diagnosis antigens, produced fair agreement in the case of A/C-2 (0.20<κ<0.40) and good agreement (0.60<κ<0.80) when A/C-7.1 was used as capture antigen. Chimera A/C-4 were excluded from further study due to loss of reactivity with OspA-specific antibodies. CONCLUSIONS: The combination of specific B-cell epitopes from OspA and OspC proteins may improve the diagnostic accuracy of serologic assays, but further studies are required to address this hypothesis.
[Mh] Termos MeSH primário: Borrelia burgdorferi/isolamento & purificação
Ensaio de Imunoadsorção Enzimática/métodos
Epitopos de Linfócito B/análise
Doença de Lyme/diagnóstico
[Mh] Termos MeSH secundário: Anticorpos Antibacterianos/análise
Anticorpos Antibacterianos/imunologia
Antígenos de Superfície/análise
Antígenos de Superfície/genética
Antígenos de Superfície/imunologia
Proteínas da Membrana Bacteriana Externa/análise
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/imunologia
Vacinas Bacterianas/análise
Vacinas Bacterianas/genética
Vacinas Bacterianas/imunologia
Borrelia burgdorferi/genética
Borrelia burgdorferi/imunologia
Epitopos de Linfócito B/imunologia
Seres Humanos
Lipoproteínas/análise
Lipoproteínas/genética
Lipoproteínas/imunologia
Doença de Lyme/imunologia
Doença de Lyme/microbiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Surface); 0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Vaccines); 0 (Epitopes, B-Lymphocyte); 0 (Lipoproteins); 0 (OspA protein)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


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[PMID]:29257832
[Au] Autor:Asmar AT; Ferreira JL; Cohen EJ; Cho SH; Beeby M; Hughes KT; Collet JF
[Ad] Endereço:de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
[Ti] Título:Communication across the bacterial cell envelope depends on the size of the periplasm.
[So] Source:PLoS Biol;15(12):e2004303, 2017 Dec.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cell envelope of gram-negative bacteria, a structure comprising an outer (OM) and an inner (IM) membrane, is essential for life. The OM and the IM are separated by the periplasm, a compartment that contains the peptidoglycan. The OM is tethered to the peptidoglycan via the lipoprotein, Lpp. However, the importance of the envelope's multilayered architecture remains unknown. Here, when we removed physical coupling between the OM and the peptidoglycan, cells lost the ability to sense defects in envelope integrity. Further experiments revealed that the critical parameter for the transmission of stress signals from the envelope to the cytoplasm, where cellular behaviour is controlled, is the IM-to-OM distance. Augmenting this distance by increasing the length of the lipoprotein Lpp destroyed signalling, whereas simultaneously increasing the length of the stress-sensing lipoprotein RcsF restored signalling. Our results demonstrate the physiological importance of the size of the periplasm. They also reveal that strict control over the IM-to-OM distance is required for effective envelope surveillance and protection, suggesting that cellular architecture and the structure of transenvelope protein complexes have been evolutionarily co-optimised for correct function. Similar strategies are likely at play in cellular compartments surrounded by 2 concentric membranes, such as chloroplasts and mitochondria.
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo
Proteínas da Membrana Bacteriana Externa/fisiologia
Periplasma/fisiologia
[Mh] Termos MeSH secundário: Membrana Celular/metabolismo
Parede Celular
Citoplasma/metabolismo
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Bactérias Gram-Negativas/metabolismo
Lipoproteínas/metabolismo
Proteínas de Membrana/metabolismo
Proteínas de Membrana/fisiologia
Peptidoglicano
Periplasma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Escherichia coli Proteins); 0 (Lipoproteins); 0 (Membrane Proteins); 0 (Peptidoglycan)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2004303



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