| [PMID]: | 29198709 |
| [Au] Autor: | Ruan B; Zhang B; Chen A; Yuan L; Liang J; Wang M; Zhang Z; Fan J; Yu X; Zhang X; Niu Z; Zheng Y; Gu S; Liu X; Du H; Wang J; Hu X; Gao L; Chen Z; Huang H; Wang X; Sun Q |
| [Ad] Endereço: | School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510000, PR China; Laboratory of Cell Engineering, Institute of Biotechnology, 20 Dongda Street, Beijing 100071, PR China. |
| [Ti] Título: | Cholesterol inhibits entotic cell-in-cell formation and actomyosin contraction. |
| [So] Source: | Biochem Biophys Res Commun;495(1):1440-1446, 2018 01 01. |
| [Is] ISSN: | 1090-2104 |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cell-in-cell structures by utilizing liposome as a vector. We found that Lipofectamine-2000, the reagent commonly used for routine transfection, could significantly reduce entotic cell-in-cell formation in a cell-specific manner, which is correlated with suppressed actomyosin contraction as indicated by reduced ß-actin expression and myosin light chain phosphorylation. The influence on cell-in-cell formation was likely dictated by specific liposome components as some liposomes affected cell-in-cell formation while some others didn't. Screening on a limited number of lipids, the major components of liposome, identified phosphatidylethanolamine (PE), stearamide (SA), lysophosphatidic acid (LPA) and cholesterol (CHOL) as the inhibitors of cell-in-cell formation. Importantly, cholesterol treatment significantly inhibited myosin light chain phosphorylation, which resembles the effect of Lipofectamine-2000, suggesting cholesterol might be partially responsible for liposomes' effects on cell-in-cell formation. Together, our findings supporting a role of membrane lipids and cholesterol in cell-in-cell formation probably via regulating actomyosin contraction. |
| [Mh] Termos MeSH primário: |
Actomiosina/metabolismo
Membrana Celular/metabolismo
Colesterol/administração & dosagem
Entose/fisiologia
Lipídeos/administração & dosagem
Lipídeos de Membrana/metabolismo
|
| [Mh] Termos MeSH secundário: |
Actomiosina/efeitos dos fármacos
Entose/efeitos dos fármacos
Seres Humanos
Células MCF-7
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Lipids); 0 (Lipofectamine); 0 (Membrane Lipids); 9013-26-7 (Actomyosin); 97C5T2UQ7J (Cholesterol) |
| [Em] Mês de entrada: | 1712 |
| [Cu] Atualização por classe: | 180105 |
[Lr] Data última revisão:
| 180105 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 171205 |
| [St] Status: | MEDLINE |
| |
|
