Base de dados : MEDLINE
Pesquisa : D10.570.755.375.760.400.800 [Categoria DeCS]
Referências encontradas : 29051 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2906 ir para página                         

  1 / 29051 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29353041
[Au] Autor:Hayashi Y; Shimamura A; Ishikawa T; Fujiwara Y; Ichi I
[Ad] Endereço:Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo, Japan.
[Ti] Título:FADS2 inhibition in essential fatty acid deficiency induces hepatic lipid accumulation via impairment of very low-density lipoprotein (VLDL) secretion.
[So] Source:Biochem Biophys Res Commun;496(2):549-555, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD). In this study, an obvious hepatic lipid accumulation was observed in EFAD mice treated with a FADS2 inhibitor. FADS2 inhibition in the EFAD state reduced secretion of very low-density lipoprotein (VLDL) and markedly diminished Mead acid in phosphatidylcholine (PC) in the liver and plasma. As the results, the amount of C20 HUFAs in hepatic and plasma PC dramatically reduced in the EFAD mice treated with a FADS2 inhibitor, whereas the decrease of C20 HUFA levels of PC in EFAD mice was not observed because of the increased Mead acid in PC. These results supposed that Mead acid in PC is important as a component of VLDL. It is possible that Mead acid plays the role of a substitute of HUFAs in VLDL secretion during EFAD.
[Mh] Termos MeSH primário: Ácidos Graxos Dessaturases/metabolismo
Ácidos Graxos Insaturados/metabolismo
Lipoproteínas VLDL/metabolismo
Fígado/metabolismo
[Mh] Termos MeSH secundário: Ácido 8,11,14-Eicosatrienoico/análogos & derivados
Ácido 8,11,14-Eicosatrienoico/metabolismo
Animais
Ácidos Graxos Dessaturases/antagonistas & inibidores
Fígado Gorduroso/metabolismo
Metabolismo dos Lipídeos
Masculino
Camundongos Endogâmicos C57BL
Oxirredução
Fosfatidilcolinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); 0 (Lipoproteins, VLDL); 0 (Phosphatidylcholines); 7324-41-6 (8,11,14-Eicosatrienoic Acid); EC 1.14.19.- (Fatty Acid Desaturases); EC 1.14.19.3 (FADS2 protein, mouse); JQS194YH3X (mead acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180122
[St] Status:MEDLINE


  2 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29413575
[Au] Autor:Takahashi R; Nakaya M; Kotaniguchi M; Shojo A; Kitamura S
[Ad] Endereço:Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai 599-8531, Japan; YMC Co., Ltd., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku, Kyoto 600-8106, Japan.
[Ti] Título:Analysis of phosphatidylethanolamine, phosphatidylcholine, and plasmalogen molecular species in food lipids using an improved 2D high-performance liquid chromatography system.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1077-1078:35-43, 2018 Mar 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phospholipids are an important class of lipids in cell membranes and food. Several high-performance liquid chromatography (HPLC) methods have been developed to analyze phospholipids at the molecular species level. We developed a two-dimensional HPLC system with a charged aerosol detector and mass spectrometry (MS) to analyze phosphatidylethanolamine (PE), phosphatidylcholine (PC), and their plasmalogens (pls) extracted from food materials. Accordingly, the phospholipid molecular species can be analyzed in a single step despite using smaller samples. We confirmed that chromatogram peaks from soybean lecithin are mostly baseline separated, assigned, and quantified (24 molecular species for PE and 27 for PC). In addition, it was confirmed that chromatograms of lipids extracted from chicken breast meat include plasmalogen peaks. The PE fraction in lipids extracted from chicken breast meat contained 17 types of ethanolamine plasmalogens, corresponding to approximately 57% of the total by weight. The PC fraction contained only four choline plasmalogens, corresponding to approximately 11% of the total weight. The composition of the pls-PC molecular species differed from that of pls-PEs. The polyunsaturated fatty acids connected at the sn-2 positions of the pls-PEs consisted of 20.5% 20:4 fatty acid and were independent of the carbon chain at the sn-1 position. However, the 18:1 fatty acid at the sn-2 position was dependent on the carbon chain at the sn-1 position.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Fosfatidilcolinas/análise
Fosfatidiletanolaminas/análise
Plasmalogênios/análise
[Mh] Termos MeSH secundário: Animais
Galinhas
Análise de Alimentos
Carne/análise
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 0 (Plasmalogens)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


  3 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29311523
[Au] Autor:Uyama M; Inoue K; Kinoshita K; Miyahara R; Yokoyama H; Nakano M
[Ad] Endereço:Shiseido Global Innovation Center.
[Ti] Título:Effect of Dialkyl Ammonium Cationic Surfactants on the Microfluidity of Membranes Containing Raft Domains.
[So] Source:J Oleo Sci;67(1):67-75, 2018.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:It has been reported that a lot of receptors localize in lipid raft domains and that the microfluidity of these domains regulates the activation of these receptors. In this study, we focused on the lipid raft and in order to evaluate the physicochemical effects of surfactants on microfluidity of lipid membranes, we used liposomes comprising of egg-yolk L-α-phosphatidylcholine, egg-yolk sphingomyelin, and cholesterol as a model of cell membranes containing raft domains. The microfluidity of the domains was characterized by fluorescence spectrometry using 1,6-diphenyl-1,3,5-hexatriene and 2-dimethylamino-6-lauroylnaphthalene. Among several surfactants, dialkylammonium-type cationic surfactants most efficiently increased the microfluidity. It is therefore concluded that (1) the electrostatic interaction between the cationic surfactant and eggPC/eggSM/cholesterol liposome could be important, (2) surfactants with alkyl chains more effectively inserted into membranes than those with acyl chains, and (3) cationic surfactants with lower T values have a greater ability to increase the fluidity.
[Mh] Termos MeSH primário: Compostos de Amônio
Membrana Celular
Fluidez de Membrana
Lipídeos de Membrana
Microdomínios da Membrana
Tensoativos
[Mh] Termos MeSH secundário: Cátions
Fenômenos Químicos
Colesterol
Gema de Ovo
Lipossomos
Fosfatidilcolinas
Espectrometria de Fluorescência
Esfingomielinas
Eletricidade Estática
Tensoativos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Cations); 0 (Liposomes); 0 (Membrane Lipids); 0 (Phosphatidylcholines); 0 (Sphingomyelins); 0 (Surface-Active Agents); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17124


  4 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29349456
[Au] Autor:Johnson QR; Mostofian B; Fuente Gomez G; Smith JC; Cheng X
[Ad] Endereço:National Institute for Mathematical and Biological Synthesis, University of Tennessee, Knoxville, TN 37996, USA.
[Ti] Título:Effects of carotenoids on lipid bilayers.
[So] Source:Phys Chem Chem Phys;20(5):3795-3804, 2018 Jan 31.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carotenoids have been found to be important in improving the integrity of biomembranes in eukaryotes. However, the molecular details of how carotenoids modulate the physical properties of biomembranes are unknown. To this end, we have conducted a series of molecular dynamics simulations of different biologically-relevant membranes in the presence of carotenoids. The carotenoid effect on the membrane was found to be specific to the identity of the carotenoid and the composition of the membrane itself. Therefore, different classes of carotenoids produce a different effect on the membrane, and different membrane phases are affected differently by carotenoids. It is apparent from our data that carotenoids do trigger the bilayer to become thinner. The mechanism by which this occurs depends on two competing factors, the ability of the lipid tails of opposing monolayers to either (1) compress or (2) interdigitate as the bilayer condenses. Indeed, carotenoids directly influence the physical properties via these two mechanisms, thus compacting the bilayer. However, the degree to which these competing mechanisms are utilized depends on the bilayer phase and the carotenoid identity.
[Mh] Termos MeSH primário: Carotenoides/metabolismo
Bicamadas Lipídicas/metabolismo
[Mh] Termos MeSH secundário: Carotenoides/química
Colesterol/química
Bicamadas Lipídicas/química
Simulação de Dinâmica Molecular
Fosfatidilcolinas/química
Zeaxantinas/química
Zeaxantinas/metabolismo
beta Caroteno/química
beta Caroteno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Phosphatidylcholines); 0 (Zeaxanthins); 01YAE03M7J (beta Carotene); 043IPI2M0K (1,2-distearoyllecithin); 36-88-4 (Carotenoids); 97C5T2UQ7J (Cholesterol); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07126d


  5 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29340383
[Au] Autor:Syryamina VN; De Zotti M; Toniolo C; Formaggio F; Dzuba SA
[Ad] Endereço:Institute of Chemical Kinetics and Combustion, RAS, Novosibirsk 630090, Russian Federation. dzuba@kinetics.nsc.ru.
[Ti] Título:Alamethicin self-assembling in lipid membranes: concentration dependence from pulsed EPR of spin labels.
[So] Source:Phys Chem Chem Phys;20(5):3592-3601, 2018 Jan 31.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antimicrobial action of the peptide antibiotic alamethicin (Alm) is commonly related to peptide self-assembling resulting in the formation of voltage-dependent channels in bacterial membranes, which induces ion permeation. To obtain a deeper insight into the mechanism of channel formation, it is useful to know the dependence of self-assembling on peptide concentration. With this aim, we studied Alm F50/5 spin-labeled analogs in a model 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, for peptide-to-lipid (P/L) ratios varying between 1/1500 and 1/100. Pulsed electron-electron double resonance (PELDOR) spectroscopy reveals that even at the lowest concentration investigated, the Alm molecules assemble into dimers. Moreover, under these conditions, electron spin echo envelope modulation (ESEEM) spectroscopy of D O-hydrated membranes shows an abrupt change from the in-plane to the trans-membrane orientation of the peptide. Therefore, we hypothesize that dimer formation and peptide reorientation are concurrent processes and represent the initial step of peptide self-assembling. By increasing peptide concentration, higher oligomers are formed. A simple kinetic model of equilibrium among monomers, dimers, and pentamers allows for satisfactorily describing the experimental PELDOR data. The inter-label distances in the oligomers obtained from PELDOR experiments become better resolved with increasing P/L ratio, thus suggesting that the supramolecular organization of the higher-order oligomers becomes more defined.
[Mh] Termos MeSH primário: Alameticina/química
Bicamadas Lipídicas/química
[Mh] Termos MeSH secundário: Alameticina/metabolismo
Sequência de Aminoácidos
Dimerização
Espectroscopia de Ressonância de Spin Eletrônica
Cinética
Bicamadas Lipídicas/metabolismo
Fosfatidilcolinas/química
Marcadores de Spin
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Phosphatidylcholines); 0 (Spin Labels); 059QF0KO0R (Water); 27061-78-5 (Alamethicin); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07298h


  6 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29177331
[Au] Autor:Ray A; Gräter F; Thukral L
[Ad] Endereço:CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, 110025, India. lipi.thukral@igib.res.in.
[Ti] Título:Probing molecular forces in multi-component physiological membranes.
[So] Source:Phys Chem Chem Phys;20(4):2155-2161, 2018 Jan 24.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological membranes are remarkably heterogeneous, composed of diverse lipid mixtures with distinct chemical structure and composition. By combining molecular dynamics simulations and the newly developed Lipid-Force Distribution Analysis (L-FDA), we explore force transmission in complex multi-component membrane models mimicking eukaryotic organelles. We found that the chemical-moiety based segmentation at membrane interfaces revealed a distinctive distribution of bonded and non-bonded forces in diverse membrane environment. Our molecular stress analysis could have far-reaching implications in describing the relationship between membrane mechanical properties and functional states of chemically distinct lipids.
[Mh] Termos MeSH primário: Bicamadas Lipídicas/química
[Mh] Termos MeSH secundário: Algoritmos
Análise por Conglomerados
Retículo Endoplasmático/química
Complexo de Golgi/química
Bicamadas Lipídicas/metabolismo
Mitocôndrias/química
Simulação de Dinâmica Molecular
Fosfatidilcolinas/química
Fosfatidiletanolaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 10015-88-0 (1-palmitoyl-2-oleoylphosphatidylethanolamine); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp05981g


  7 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27770747
[Au] Autor:Lee H
[Ad] Endereço:Department of Chemical Engineering, Dankook University, Yongin, 448-701, South Korea. Electronic address: leeh@dankook.ac.kr.
[Ti] Título:Effect of polyelectrolyte size on multilayer conformation and dynamics at different temperatures and salt concentrations.
[So] Source:J Mol Graph Model;70:246-252, 2016 11.
[Is] ISSN:1873-4243
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyelectrolyte bilayers, which consist of poly-l-lysine (PLL) and hyaluronic acid (HA) were simulated with lipid membranes at different temperatures and ion concentrations. Starting with the sequential deposition of PLL and HA above the membrane surface, PLL and HA become completely mixed, leading to the formation of stable bilayers. PLL/HA bilayers are thicker at higher salt concentration because of weakened electrostatic interactions between PLLs and membrane lipids, in agreement with experiments. This salt effect decreases as PLL size increases. Also, bilayers become thinner at higher temperature because of the increased surface area of membrane. In particular, regardless of temperature and salt concentration, larger PLLs induce thicker bilayers, although larger PLLs have lower diffusivities than do smaller ones. Bilayers with larger PLLs show larger vacancy (more water) inside the bilayer, indicating that larger PLLs are less densely stacked on membrane surface than do smaller ones and thus form the thicker bilayer. These findings show the lower diffusivity of larger polyelectrolytes, which supports the experimental observation regarding the restricted diffusion of large polymers, and also imply the dependence of bilayer thickness on the polymer size.
[Mh] Termos MeSH primário: Conformação Molecular
Polieletrólitos/química
Cloreto de Sódio/química
Temperatura Ambiente
[Mh] Termos MeSH secundário: Ânions
Difusão
Ácido Hialurônico/química
Bicamadas Lipídicas/química
Lipídeos/química
Simulação de Dinâmica Molecular
Fosfatidilcolinas/química
Polilisina/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anions); 0 (Lipid Bilayers); 0 (Lipids); 0 (Phosphatidylcholines); 0 (Polyelectrolytes); 059QF0KO0R (Water); 25104-18-1 (Polylysine); 451W47IQ8X (Sodium Chloride); 9004-61-9 (Hyaluronic Acid); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  8 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29388543
[Au] Autor:Park S; Choi SJ; Won SM; Yoon JH
[Ad] Endereço:Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
[Ti] Título:Jannaschia confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring.
[So] Source:Int J Syst Evol Microbiol;68(2):669-674, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated JSSK-16 , was isolated from the place where the ocean and a freshwater spring meet at Jeju Island, South Korea. Strain JSSK-16 grew optimally at 30 °C, at pH 6.5-8.0 and in the presence of 2.0-4.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JSSK-16 joined the clade comprising the type strains of Jannaschia species. Strain JSSK-16 exhibited 16S rRNA gene sequence similarity values of 97.5 and 97.1 % to the type strains of Jannaschia donghaensis and Jannaschia faecimaris, respectively, and of 94.1-96.6 % to the type strains of the other Jannaschia species. Strain JSSK-16 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c, 11-methyl C18 : 1ω7c and C18 : 0 as the major fatty acids. The major polar lipids detected in strain JSSK-16 were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JSSK-16T was 68.8 mol% and its DNA-DNA relatedness values with the type strains of J. donghaensis and J. faecimaris were 18 and 12, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain JSSK-16 is separated from recognized species of the genus Jannaschia. On the basis of the data presented, strain JSSK-16 is considered to represent a novel species of the genus Jannaschia, for which the name Jannaschia confluentis sp. nov. is proposed. The type strain is JSSK-16T (=KACC 19436 =KCTC 62137 =NBRC 113018 ).
[Mh] Termos MeSH primário: Água Doce/microbiologia
Filogenia
Rhodobacteraceae/classificação
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfatidilcolinas/química
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
República da Coreia
Rhodobacteraceae/genética
Rhodobacteraceae/isolamento & purificação
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002564


  9 / 29051 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29210387
[Au] Autor:Deplazes E; Poger D; Cornell B; Cranfield CG
[Ad] Endereço:School of Biomedical Sciences, Curtin Health Innovation Research Institute and Curtin Institute for Computation, Curtin University, Perth, WA 6845, Australia. evelyne.deplazes@curtin.edu.au.
[Ti] Título:The effect of hydronium ions on the structure of phospholipid membranes.
[So] Source:Phys Chem Chem Phys;20(1):357-366, 2017 Dec 20.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work seeks to identify the mechanisms by which hydronium ions (H O ) modulate the structure of phospholipid bilayers by studying the interactions of H O with phospholipids at the molecular level. For this, we carried out multiple microsecond-long unrestrained molecular dynamics (MD) simulations of a POPC bilayer at different H O concentrations. The results show that H O accumulates at the membrane surface where it displaces water and forms strong and long-lived hydrogen bonds with the phosphate and carbonyl oxygens in phospholipids. This results in a concentration-dependent reduction of the area per lipid and an increase in bilayer thickness. This study provides an important molecular-level insight into the mechanism of how H O modulates the structure of biological membranes and is a critical step towards a better understanding of the effect of low pH on mammalian and bacterial membranes.
[Mh] Termos MeSH primário: Bicamadas Lipídicas/química
Oniocompostos/química
Fosfolipídeos/química
[Mh] Termos MeSH secundário: Ligações de Hidrogênio
Simulação de Dinâmica Molecular
Fosfatidilcolinas/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Onium Compounds); 0 (Phosphatidylcholines); 0 (Phospholipids); 059QF0KO0R (Water); 5046UKT60S (hydronium ion); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06776c


  10 / 29051 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:29363925
[Au] Autor:Bykov MI; Basov AA; Esaulenko EE; Kurzanov AN
[Ti] Título:[Experimental study of influence of lipophilic products of phytogenic origin on lipid metabolism in rats].
[So] Source:Vopr Pitan;84(5):31-8, 2015.
[Is] ISSN:0042-8833
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The article presents the results of biochemical evaluation of metabolic effects of lipophilic products of plant origin among which such oils as linseed, black nuts and walnuts oils as well as medicine «Phosphogliv¼ were selected as the most promising ones. The influence of the studied substances on lipid metabolism in experiment on male rats (170­220 g body weight) with modeled acute hepatotoxicity with carbon tetrachloride (that was achieved by subcutaneous injection of 50% oil solution of carbon tetrachloride ­ 0.5 ml/100 g of the body mass once a day during 3 days) has been investigated. Liver function was assessed by triacylglycerols content in the serum, total, esterified and nonesterified cholesterol, cholesterol in the lipoproteins of high, low and very low density, as well as by the nonesterified cholesterol and phospholipids content in the hemolysate of red blood cells. Carbon tetrachloride hepatotoxic damage was accompanied by the development of severe hypercholesterolemia associated both with the increase in total cholesterol and its content in low density lipoproteins alongside the reducing of the cholesterol concentration in high density lipoproteins, resulted in secondary dyslipoproteinemia. Inhibition of the esterification of cholesterol processes as well as the decrease in the triacylglycerols concentration was observed. It is connected with the triacylglycerols endogenous synthesis blocking in the liver, resulted from its toxic damage. It is also confirmed by cholesterol content reducing in the lipoproteins of very low density. In erythrocytes of rats with CCl4 intoxication phospholipid content decreased while the amount of nonesterified cholesterol that is a component of cell membranes, influencing the proteins and lipids diffusion, which reduces the mobility of the fatty acid residues of phospholipids, increased. The injection of the black nuts and walnuts oils as well as flax oil (intragastric injections 0.2 ml daily in the morning before the main feeding from the 7th to the 30th day of the experiment, n=25 in each group) to rats with liver failure induced by carbon tetrachloride, contributed to the partial restoration of liver tissue structure and statistically reliable decrease of lipid metabolism. Decrease in the total cholesterol content by 17.5% in the group of animals treated with linseed oil was observed; LDL cholesterol also decreased under the influence of walnuts and black nuts oils by 36.7 and 40.6% respectively. The increase in the content of phospholipids in erythrocytes of rats when administered by the studied lipophilic products has made it possible to prove the improvement of the cell membranes rheological properties. The results of the study of the influence of linseed, black nuts and walnuts oils as well as medicine «Phosphogliv¼ on animals with hepatotoxicity by CCl4 have proved positive effect of these lipophilic substances on lipid metabolism.
[Mh] Termos MeSH primário: Ração Animal
Ácido Glicirrízico/farmacologia
Óleo de Semente do Linho/farmacologia
Metabolismo dos Lipídeos/efeitos dos fármacos
Fígado/metabolismo
Fosfatidilcolinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Intoxicação por Tetracloreto de Carbono/metabolismo
Intoxicação por Tetracloreto de Carbono/patologia
Combinação de Medicamentos
Fígado/patologia
Masculino
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Phosphatidylcholines); 0 (Phosphogliv); 6FO62043WK (Glycyrrhizic Acid); 8001-26-1 (Linseed Oil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE



página 1 de 2906 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde