Base de dados : MEDLINE
Pesquisa : D12.125.067 [Categoria DeCS]
Referências encontradas : 158 [refinar]
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[PMID]:27500592
[Au] Autor:Wang X; Huang W; Liu J; Yang Z; Huang B
[Ad] Endereço:College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
[Ti] Título:Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress.
[So] Source:Plant Biotechnol J;15(2):237-248, 2017 Feb.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heat stress transcription factors (HSFs) compose a large gene family, and different members play differential roles in regulating plant responses to abiotic stress. The objectives of this study were to identify and characterize an A2-type HSF, FaHsfA2c, in a cool-season perennial grass tall fescue (Festuca arundinacea Schreb.) for its association with heat tolerance and to determine the underlying physiological functions and regulatory mechanisms of FaHsfA2c imparting plant tolerance to heat stress. FaHsfA2c was localized in nucleus and exhibited a rapid transcriptional increase in leaves and roots during early phase of heat stress. Ectopic expression of FaHsfA2c improved basal and acquired thermotolerance in wild-type Arabidopsis and also restored heat-sensitive deficiency of hsfa2 mutant. Overexpression of FaHsfA2c in tall fescue enhanced plant tolerance to heat by triggering transcriptional regulation of heat-protective gene expression, improving photosynthetic capacity and maintaining plant growth under heat stress. Our results indicated that FaHsfA2c acted as a positive regulator conferring thermotolerance improvement in Arabidopsis and tall fescue, and it could be potentially used as a candidate gene for genetic modification and molecular breeding to develop heat-tolerant cool-season grass species.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/fisiologia
Festuca/fisiologia
Proteínas de Choque Térmico/fisiologia
Proteínas de Plantas/fisiologia
Termotolerância/genética
Fatores de Transcrição/fisiologia
[Mh] Termos MeSH secundário: Aminoácidos Acídicos
Arabidopsis/genética
Proteínas de Arabidopsis
Clorofila/metabolismo
Embaralhamento de DNA
Proteínas de Ligação a DNA/genética
Festuca/genética
Festuca/crescimento & desenvolvimento
Genes de Plantas
Fatores de Transcrição de Choque Térmico
Proteínas de Choque Térmico/genética
Temperatura Alta
Mutação
Fenótipo
Filogenia
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Estações do Ano
Alinhamento de Sequência
Estresse Fisiológico/genética
Taxa de Sobrevida
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Arabidopsis Proteins); 0 (DNA-Binding Proteins); 0 (HSFA2 protein, Arabidopsis); 0 (Heat Shock Transcription Factors); 0 (Heat-Shock Proteins); 0 (Plant Proteins); 0 (Transcription Factors); 1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12609


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[PMID]:26046278
[Au] Autor:Wu X; Zhao X; Li Y; Yang T; Yan X; Wang K
[Ad] Endereço:Department of Pediatric Dentistry, The Hospital of Stomatology, Jilin University, Changchun 130021, China.
[Ti] Título:In situ synthesis carbonated hydroxyapatite layers on enamel slices with acidic amino acids by a novel two-step method.
[So] Source:Mater Sci Eng C Mater Biol Appl;54:150-7, 2015 Sep.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In situ fabrication of carbonated hydroxyapatite (CHA) remineralization layer on an enamel slice was completed in a novel, biomimetic two-step method. First, a CaCO3 layer was synthesized on the surface of demineralized enamel using an acidic amino acid (aspartic acid or glutamate acid) as a soft template. Second, at the same concentration of the acidic amino acid, rod-like carbonated hydroxyapatite was produced with the CaCO3 layer as a sacrificial template and a reactant. The morphology, crystallinity and other physicochemical properties of the crystals were characterized using field emission scanning electron microscopy (FESEM), Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD) and energy-dispersive X-ray analysis (EDAX), respectively. Acidic amino acid could promote the uniform deposition of hydroxyapatite with rod-like crystals via absorption of phosphate and carbonate ions from the reaction solution. Moreover, compared with hydroxyapatite crystals coated on the enamel when synthesized by a one-step method, the CaCO3 coating that was synthesized in the first step acted as an active bridge layer and sacrificial template. It played a vital role in orienting the artificial coating layer through the template effect. The results show that the rod-like carbonated hydroxyapatite crystals grow into bundles, which are similar in size and appearance to prisms in human enamel, when using the two-step method with either aspartic acid or acidic glutamate (20.00 mmol/L).
[Mh] Termos MeSH primário: Aminoácidos Acídicos/química
Durapatita/síntese química
Remineralização Dentária/métodos
[Mh] Termos MeSH secundário: Ácido Aspártico/química
Biomimética
Carbonato de Cálcio/síntese química
Cristalização
Esmalte Dentário/química
Seres Humanos
Microscopia Eletrônica de Varredura
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 30KYC7MIAI (Aspartic Acid); 91D9GV0Z28 (Durapatite); H0G9379FGK (Calcium Carbonate)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150606
[Lr] Data última revisão:
150606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150606
[St] Status:MEDLINE


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[PMID]:25955787
[Au] Autor:Chen Z; Hu Y; Hong J; Hu J; Yang W; Xiang F; Yang F; Xie Z; Cao Z; Li W; Lin D; Wu Y
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
[Ti] Título:Toxin acidic residue evolutionary function-guided design of de novo peptide drugs for the immunotherapeutic target, the Kv1.3 channel.
[So] Source:Sci Rep;5:9881, 2015 May 08.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During the long-term evolution of animal toxins acting on potassium channels, the acidic residues can orientate the toxin binding interfaces by adjusting the molecular polarity. Based on the evolutionary function of toxin acidic residues, de novo peptide drugs with distinct binding interfaces were designed for the immunotherapeutic target, the Kv1.3 channel. Using a natural basic toxin, BmKTX, as a template, which contains 2 acidic residues (Asp19 and Asp33), we engineered two new peptides BmKTX-19 with 1 acidic residue (Asp33), and BmKTX-196 with 2 acidic residues (Asp6 and Asp33) through only adjusting acidic residue distribution for reorientation of BmKTX binding interface. Pharmacological experiments indicated that BmKTX-19 and BmKTX-196 peptides were specific inhibitors of the Kv1.3 channel and effectively suppressed cytokine secretion. In addition to the structural similarity between the designed and native peptides, both experimental alanine-scanning mutagenesis and computational simulation further indicated that the binding interface of wild-type BmKTX was successfully reoriented in BmKTX-19 and BmKTX-196, which adopted distinct toxin surfaces as binding interfaces. Together, these findings indicate not only the promising prospect of BmKTX-19 and BmKTX-196 as drug candidates but also the desirable feasibility of the evolution-guided peptide drug design for discovering numerous peptide drugs for the Kv1.3 channel.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/toxicidade
Desenho de Drogas
Evolução Molecular
Imunoterapia
Peptídeos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Citocinas/secreção
Células HEK293
Seres Humanos
Canal de Potássio Kv1.3/antagonistas & inibidores
Canal de Potássio Kv1.3/química
Modelos Moleculares
Dados de Sequência Molecular
Mutagênese/efeitos dos fármacos
Bloqueadores dos Canais de Potássio/farmacologia
Ligação Proteica/efeitos dos fármacos
Venenos de Escorpião/química
Soluções
Linfócitos T/efeitos dos fármacos
Linfócitos T/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Cytokines); 0 (KTX toxin, Buthus); 0 (Kv1.3 Potassium Channel); 0 (Peptides); 0 (Potassium Channel Blockers); 0 (Scorpion Venoms); 0 (Solutions)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150509
[St] Status:MEDLINE
[do] DOI:10.1038/srep09881


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[PMID]:25589253
[Au] Autor:Xu X; Jia Z; Shu Y; Liu L
[Ad] Endereço:School of Pharmaceutical Sciences, Southern Medical University, 1838, North Guangzhou Avenue, Guangzhou 510515, China.
[Ti] Título:Dynamic pH junction-sweeping technique for on-line concentration of acidic amino acids in human serum by capillary electrophoresis with indirect UV detection.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;980:20-7, 2015 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Glutamic acid (Glu) and aspartic acid (Asp), as two important neurotransmitters, have been the focus of increasingly intense research over the past several years. Glu and Asp are present in biological fluids such as serum at trace levels, but complex components in biological matrices make it difficult to determine them in biological samples. In this paper, a sensitive and simple method coupled with indirect UV detection, using benzoic acid (BA) as the UV-absorbing probe, was developed and validated for the quantitative determination of Glu and Asp in human serum and Compound Amino Acid Injection-18 AA. The method combines a dynamic pH junction with a sweeping technique using ß-cyclodextrin (ß-CD) as the complexing agent for sweeping. Employing this proposed method, low detection limits of 0.061µg/mL for Glu and 0.032µg/mL for Asp were obtained. The sensitivity was improved 30- and 55-fold for Glu and Asp compared to conventional CE method. Standard curves were linear (r>0.999) over the concentration range of 0.1-8.0µg/mL. To further improve the resolution of Asp from interfering substances in human serum, 6% (v/v) methanol was added to the sample matrix, and resulted in the detection limits of 0.125µg/mL for Glu and 0.057µg/mL for Asp. With a simple precipitation of protein, the method has been successfully applied to the analysis of human serum, and the recoveries (82% for Glu and 87% for Asp) were achieved with relative standard deviations of 1.9% and 2.0%, respectively.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/sangue
Eletroforese Capilar/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150121
[Lr] Data última revisão:
150121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150116
[St] Status:MEDLINE


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[PMID]:25588312
[Au] Autor:Takahashi T; Tanaka T; Tsushima Y; Muragaki K; Uehara K; Takeuchi S; Maeda H; Yamagata Y; Nakayama M; Yoshimi A; Abe K
[Ad] Endereço:Microbial Genomics Laboratory, New Industry Creation Hatchery Center, Tohoku University, Sendai, Miyagi, 981-8555, Japan.
[Ti] Título:Ionic interaction of positive amino acid residues of fungal hydrophobin RolA with acidic amino acid residues of cutinase CutL1.
[So] Source:Mol Microbiol;96(1):14-27, 2015 Apr.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hydrophobins are amphipathic proteins secreted by filamentous fungi. When the industrial fungus Aspergillus oryzae is grown in a liquid medium containing the polyester polybutylene succinate co-adipate (PBSA), it produces RolA, a hydrophobin, and CutL1, a PBSA-degrading cutinase. Secreted RolA attaches to the surface of the PBSA particles and recruits CutL1, which then condenses on the particles and stimulates the hydrolysis of PBSA. Here, we identified amino acid residues that are required for the RolA-CutL1 interaction by using site-directed mutagenesis. We quantitatively analyzed kinetic profiles of the interactions between RolA variants and CutL1 variants by using a quartz crystal microbalance (QCM). The QCM analyses revealed that Asp142, Asp171 and Glu31, located on the hydrophilic molecular surface of CutL1, and His32 and Lys34, located in the N-terminus of RolA, play crucial roles in the RolA-CutL1 interaction via ionic interactions. RolA immobilized on a QCM electrode strongly interacted with CutL1 (K(D) = 6.5 nM); however, RolA with CutL1 variants, or RolA variants with CutL1, showed markedly larger KD values, particularly in the interaction between the double variant RolA-H32S/K34S and the triple variant CutL1-E31S/D142S/D171S (K(D) = 78.0 nM). We discuss a molecular prototype model of hydrophobin-based enzyme recruitment at the solid-water interface.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/metabolismo
Aspergillus oryzae/genética
Aspergillus oryzae/metabolismo
Hidrolases de Éster Carboxílico/metabolismo
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Hidrolases de Éster Carboxílico/química
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Interações Hidrofóbicas e Hidrofílicas
Íons
Modelos Moleculares
Mutagênese Sítio-Dirigida
Poliésteres/metabolismo
Polímeros/metabolismo
Técnicas de Microbalança de Cristal de Quartzo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Fungal Proteins); 0 (Ions); 0 (Polyesters); 0 (Polymers); 0 (polybutylene succinate-co-adipate); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (cutinase)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150330
[Lr] Data última revisão:
150330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150116
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.12915


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[PMID]:25461159
[Au] Autor:Zhang Q; Song C; Zhao T; Fu HW; Wang HZ; Wang YJ; Kong DM
[Ad] Endereço:State Key Laboratory of Medicinal Chemical Biology, Research Centre for Analytical Sciences, Nankai University, Tianjin 300071, P.R. China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, P.R. China.
[Ti] Título:Photoluminescent sensing for acidic amino acids based on the disruption of graphene quantum dots/europium ions aggregates.
[So] Source:Biosens Bioelectron;65:204-10, 2015 Mar 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A simple mix-and-detect photoluminescence method was developed for the turn-on detection of acidic amino acids. To achieve this, graphene quantum dots (GQDs), which emit both down-conversion and up-conversion photoluminescence were prepared by solvothermal synthesis. The carboxylic acid-rich surface not only increases the water solubility of the prepared GQDs, but also makes Eu(3+)-triggered GQDs aggregation possible, thus causing the photoluminescence quenching of GQDs. The quenched photoluminescence can be recovered by the competition between acidic amino acids and GQDs for Eu(3+). Under optimized conditions, sensitive and specific acidic amino acids quantitation can be achieved by utilizing the changes in either down-conversion or up-conversion photoluminescence. Up-conversion mode gives a little lower detection limit than the down-conversion one. Nearly overlapped calibration curves were obtained for the two acidic amino acids, glutamic acid (Glu) and aspartic acid (Asp), thus suggesting that the proposed method can be used not only for the quantitation of individual acidic amino acids, but also for the detection of total amount of them.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/sangue
Európio/química
Grafite/química
Medições Luminescentes/métodos
Pontos Quânticos/química
[Mh] Termos MeSH secundário: Aminoácidos Acídicos/análise
Animais
Cátions/química
Bovinos
Limite de Detecção
Pontos Quânticos/ultraestrutura
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Cations); 444W947O8O (Europium); 7782-42-5 (Graphite)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170125
[Lr] Data última revisão:
170125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25315822
[Au] Autor:Freeman AD; Liu Y; Déclais AC; Gartner A; Lilley DM
[Ad] Endereço:Cancer Research UK Nucleic Acid Structure Research Group, MSI/WTB Complex, The University of Dundee, Dow Street, Dundee DD1 5EH, UK.
[Ti] Título:GEN1 from a thermophilic fungus is functionally closely similar to non-eukaryotic junction-resolving enzymes.
[So] Source:J Mol Biol;426(24):3946-59, 2014 Dec 12.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Processing of Holliday junctions is essential in recombination. We have identified the gene for the junction-resolving enzyme GEN1 from the thermophilic fungus Chaetomium thermophilum and expressed the N-terminal 487-amino-acid section. The protein is a nuclease that is highly selective for four-way DNA junctions, cleaving 1nt 3' to the point of strand exchange on two strands symmetrically disposed about a diagonal axis. CtGEN1 binds to DNA junctions as a discrete homodimer with nanomolar affinity. Analysis of the kinetics of cruciform cleavage shows that cleavage of the second strand occurs an order of magnitude faster than the first cleavage so as to generate a productive resolution event. All these properties are closely similar to those described for bacterial, phage and mitochondrial junction-resolving enzymes. CtGEN1 is also similar in properties to the human enzyme but lacks the problems with aggregation that currently prevent detailed analysis of the latter protein. CtGEN1 is thus an excellent enzyme with which to engage in biophysical and structural analysis of eukaryotic GEN1.
[Mh] Termos MeSH primário: Chaetomium/enzimologia
DNA Cruciforme/metabolismo
Proteínas Fúngicas/metabolismo
Resolvases de Junção Holliday/metabolismo
[Mh] Termos MeSH secundário: Algoritmos
Sequência de Aminoácidos
Aminoácidos Acídicos/genética
Aminoácidos Acídicos/metabolismo
Sequência de Bases
Ligação Competitiva
Chaetomium/genética
DNA Cruciforme/química
DNA Cruciforme/genética
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Resolvases de Junção Holliday/classificação
Resolvases de Junção Holliday/genética
Hidrólise
Cinética
Modelos Genéticos
Modelos Moleculares
Dados de Sequência Molecular
Mutação
Conformação de Ácido Nucleico
Filogenia
Ligação Proteica
Multimerização Proteica
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (DNA, Cruciform); 0 (Fungal Proteins); EC 3.1.21.- (Holliday Junction Resolvases)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141016
[St] Status:MEDLINE


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[PMID]:24901998
[Au] Autor:Xiao Q; Cui Y
[Ad] Endereço:From the Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.
[Ti] Título:Acidic amino acids in the first intracellular loop contribute to voltage- and calcium- dependent gating of anoctamin1/TMEM16A.
[So] Source:PLoS One;9(6):e99376, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anoctamin1 (Ano1, or TMEM16A) is a Ca2+-activated chloride channel that is gated by both voltage and Ca2+. We have previously identified that the first intracellular loop that contains a high density of acidic residues mediates voltage- and calcium-dependent gating of Ano1. Mutation of the four consecutive glutamates (444EEEE447) inhibits the voltage-dependent activation of Ano1, whereas deletion of these residues decreases apparent Ca2+ sensitivity. In the present study, we further found that deletion of 444EEEEEAVKD452 produced a more than 40-fold decrease in the apparent Ca2+ sensitivity with altered activation kinetics. We then systematically mutated each acidic residue into alanine, and analyzed the voltage- and calcium dependent activation of each mutation. Activation kinetics of wild type Ano1 consisted of a fast component (τfast) that represented voltage-dependent mode, and a slow component (τslow) that reflected the Ca2+-dependent modal gating. E444A, E445A, E446A, E447A, E448A, and E457A mutations showed a decrease in the τfast, significantly inhibited voltage-dependent activation of Ano1 in the absence of Ca2+, and greatly shifted the G-V curve to the right, suggesting that these glutamates are involved in voltage-gating of Ano1. Furthermore, D452A, E464A, E470A, and E475A mutations that did not alter voltage-dependent activation of the channel, significantly decreased Ca2+ dependence of G-V curve, exhibited an increase in the τslow, and produced a 2-3 fold decrease in the apparent Ca2+ sensitivity, suggesting that these acidic residues are involved in Ca2+-dependent gating of the channel. Our data show that acidic residues in the first intracellular loop are the important structural determinant that couples the voltage and calcium dependent gating of Ano1.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/metabolismo
Cálcio/metabolismo
Canais de Cloreto/metabolismo
Proteínas de Neoplasias/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Sequência de Aminoácidos
Anoctamina-1
Canais de Cloreto/química
Canais de Cloreto/genética
Células HEK293
Seres Humanos
Cinética
Mutação
Proteínas de Neoplasias/química
Proteínas de Neoplasias/genética
Técnicas de Patch-Clamp
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ANO1 protein, human); 0 (Amino Acids, Acidic); 0 (Anoctamin-1); 0 (Chloride Channels); 0 (Neoplasm Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0099376


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[PMID]:24840903
[Au] Autor:Wang L; Murai Y; Yoshida T; Okamoto M; Tachrim ZP; Hashidoko Y; Hashimoto M
[Ad] Endereço:Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan.
[Ti] Título:Utilization of acidic α-amino acids as acyl donors: an effective stereo-controllable synthesis of aryl-keto α-amino acids and their derivatives.
[So] Source:Molecules;19(5):6349-67, 2014 May 16.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Aryl-keto-containing α-amino acids are of great importance in organic chemistry and biochemistry. They are valuable intermediates for the construction of hydroxyl α-amino acids, nonproteinogenic α-amino acids, as well as other biofunctional components. Friedel-Crafts acylation is an effective method to prepare aryl-keto derivatives. In this review, we summarize the preparation of aryl-keto containing α-amino acids by Friedel-Crafts acylation using acidic α-amino acids as acyl-donors and Lewis acids or Brönsted acids as catalysts.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/química
Ácidos de Lewis/química
Mesilatos/química
[Mh] Termos MeSH secundário: Acilação
Aminoácidos Acídicos/metabolismo
Catálise
Ácidos de Lewis/metabolismo
Mesilatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Lewis Acids); 0 (Mesylates); JE2SY203E8 (trifluoromethanesulfonic acid)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140520
[Lr] Data última revisão:
140520
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140521
[St] Status:MEDLINE
[do] DOI:10.3390/molecules19056349


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[PMID]:24778431
[Au] Autor:Pless SA; Elstone FD; Niciforovic AP; Galpin JD; Yang R; Kurata HT; Ahern CA
[Ad] Endereço:Department of Anesthesiology, Pharmacology and Therapeutics, and 2 Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
[Ti] Título:Asymmetric functional contributions of acidic and aromatic side chains in sodium channel voltage-sensor domains.
[So] Source:J Gen Physiol;143(5):645-56, 2014 May.
[Is] ISSN:1540-7748
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Voltage-gated sodium (NaV) channels mediate electrical excitability in animals. Despite strong sequence conservation among the voltage-sensor domains (VSDs) of closely related voltage-gated potassium (KV) and NaV channels, the functional contributions of individual side chains in Nav VSDs remain largely enigmatic. To this end, natural and unnatural side chain substitutions were made in the S2 hydrophobic core (HC), the extracellular negative charge cluster (ENC), and the intracellular negative charge cluster (INC) of the four VSDs of the skeletal muscle sodium channel isoform (NaV1.4). The results show that the highly conserved aromatic side chain constituting the S2 HC makes distinct functional contributions in each of the four NaV domains. No obvious cation-pi interaction exists with nearby S4 charges in any domain, and natural and unnatural mutations at these aromatic sites produce functional phenotypes that are different from those observed previously in Kv VSDs. In contrast, and similar to results obtained with Kv channels, individually neutralizing acidic side chains with synthetic derivatives and with natural amino acid substitutions in the INC had little or no effect on the voltage dependence of activation in any of the four domains. Interestingly, countercharge was found to play an important functional role in the ENC of DI and DII, but not DIII and DIV. These results suggest that electrostatic interactions with S4 gating charges are unlikely in the INC and only relevant in the ENC of DI and DII. Collectively, our data highlight domain-specific functional contributions of highly conserved side chains in NaV VSDs.
[Mh] Termos MeSH primário: Aminoácidos Acídicos/química
Aminoácidos Aromáticos/química
Ativação do Canal Iônico
Proteínas Musculares/química
Canais de Sódio/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Substituição de Aminoácidos
Aminoácidos Acídicos/genética
Aminoácidos Aromáticos/genética
Animais
Potenciais da Membrana
Dados de Sequência Molecular
Proteínas Musculares/genética
Proteínas Musculares/metabolismo
Estrutura Terciária de Proteína
Ratos
Canais de Sódio/genética
Canais de Sódio/metabolismo
Xenopus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids, Acidic); 0 (Amino Acids, Aromatic); 0 (Muscle Proteins); 0 (Scn4a protein, rat); 0 (Sodium Channels)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140430
[St] Status:MEDLINE
[do] DOI:10.1085/jgp.201311036



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