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  1 / 20503 MEDLINE  
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[PMID]:28461448
[Au] Autor:Borchert AJ; Downs DM
[Ad] Endereço:Department of Microbiology, University of Georgia, Athens, Georgia, USA.
[Ti] Título:The Response to 2-Aminoacrylate Differs in Escherichia coli and Salmonella enterica, despite Shared Metabolic Components.
[So] Source:J Bacteriol;199(14), 2017 07 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The metabolic network of an organism includes the sum total of the biochemical reactions present. In microbes, this network has an impeccable ability to sense and respond to perturbations caused by internal or external stimuli. The metabolic potential (i.e., network structure) of an organism is often drawn from the genome sequence, based on the presence of enzymes deemed to indicate specific pathways. and are members of the family of Gram-negative bacteria that share the majority of their metabolic components and regulatory machinery as the "core genome." In , the ability of the enamine intermediate 2-aminoacrylate (2AA) to inactivate a number of pyridoxal 5'-phosphate (PLP)-dependent enzymes has been established In this study, 2AA metabolism and the consequences of its accumulation were investigated in The data showed that despite the conservation of all relevant enzymes, and differed in both the generation and detrimental consequences of 2AA. In total, these findings suggest that the structure of the metabolic network surrounding the generation and response to endogenous 2AA stress differs between and This work compared the metabolic networks surrounding the endogenous stressor 2-aminoacrylate in two closely related members of the The data showed that despite the conservation of all relevant enzymes in this metabolic node, the two closely related organisms diverged in their metabolic network structures. This work highlights how a set of conserved components can generate distinct network architectures and how this can impact the physiology of an organism. This work defines a model to expand our understanding of the 2-aminoacrylate stress response and the differences in metabolic structures and cellular milieus between and .
[Mh] Termos MeSH primário: Acrilatos/farmacologia
Proteínas de Bactérias/metabolismo
Escherichia coli/efeitos dos fármacos
Salmonella enterica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenina/farmacologia
Ácido Aspártico/farmacologia
Proteínas de Bactérias/genética
Escherichia coli/metabolismo
Deleção de Genes
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Regulação Enzimológica da Expressão Gênica/fisiologia
L-Serina Desidratase/genética
L-Serina Desidratase/metabolismo
Salmonella enterica/metabolismo
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Acrylates); 0 (Bacterial Proteins); 30KYC7MIAI (Aspartic Acid); EC 4.3.1.17 (L-Serine Dehydratase); JAC85A2161 (Adenine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 20503 MEDLINE  
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[PMID]:29311556
[Au] Autor:Springsteen G; Yerabolu JR; Nelson J; Rhea CJ; Krishnamurthy R
[Ad] Endereço:Department of Chemistry, Furman University, Greenville, SC, 29613, USA.
[Ti] Título:Linked cycles of oxidative decarboxylation of glyoxylate as protometabolic analogs of the citric acid cycle.
[So] Source:Nat Commun;9(1):91, 2018 01 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The development of metabolic approaches towards understanding the origins of life, which have focused mainly on the citric acid (TCA) cycle, have languished-primarily due to a lack of experimentally demonstrable and sustainable cycle(s) of reactions. We show here the existence of a protometabolic analog of the TCA involving two linked cycles, which convert glyoxylate into CO and produce aspartic acid in the presence of ammonia. The reactions proceed from either pyruvate, oxaloacetate or malonate in the presence of glyoxylate as the carbon source and hydrogen peroxide as the oxidant under neutral aqueous conditions and at mild temperatures. The reaction pathway demonstrates turnover under controlled conditions. These results indicate that simpler versions of metabolic cycles could have emerged under potential prebiotic conditions, laying the foundation for the appearance of more sophisticated metabolic pathways once control by (polymeric) catalysts became available.
[Mh] Termos MeSH primário: Dióxido de Carbono/química
Glioxilatos/química
Modelos Químicos
Origem da Vida
Ácido Oxaloacético/química
Ácido Pirúvico/química
[Mh] Termos MeSH secundário: Amônia/química
Ácido Aspártico/química
Descarboxilação
Peróxido de Hidrogênio/química
Concentração de Íons de Hidrogênio
Cinética
Malonatos/química
Redes e Vias Metabólicas
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Glyoxylates); 0 (Malonates); 142M471B3J (Carbon Dioxide); 2F399MM81J (Oxaloacetic Acid); 30KYC7MIAI (Aspartic Acid); 7664-41-7 (Ammonia); 8558G7RUTR (Pyruvic Acid); 9KX7ZMG0MK (malonic acid); BBX060AN9V (Hydrogen Peroxide); JQ39C92HH6 (glyoxylic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02591-0


  3 / 20503 MEDLINE  
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[PMID]:29338047
[Au] Autor:Hsia HP; Yang YH; Szeto WC; Nilsson BE; Lo CY; Ng AK; Fodor E; Shaw PC
[Ad] Endereço:Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
[Ti] Título:Amino acid substitutions affecting aspartic acid 605 and valine 606 decrease the interaction strength between the influenza virus RNA polymerase PB2 '627' domain and the viral nucleoprotein.
[So] Source:PLoS One;13(1):e0191226, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The influenza virus RNA genome is transcribed and replicated in the context of the viral ribonucleoprotein (vRNP) complex by the viral RNA polymerase. The nucleoprotein (NP) is the structural component of the vRNP providing a scaffold for the viral RNA. In the vRNP as well as during transcription and replication the viral polymerase interacts with NP but it is unclear which parts of the polymerase and NP mediate these interactions. Previously the C-terminal '627' domain (amino acids 538-693) of PB2 was shown to interact with NP. Here we report that a fragment encompassing amino acids 146-185 of NP is sufficient to mediate this interaction. Using NMR chemical shift perturbation assays we show that amino acid region 601 to 607 of the PB2 '627' domain interacts with this fragment of NP. Substitutions of these PB2 amino acids resulted in diminished RNP activity and surface plasmon resonance assays showed that amino acids D605 was essential for the interaction with NP and V606 may also play a partial role in the interaction. Collectively these results reveal a possible interaction surface between NP and the PB2 subunit of the RNA polymerase complex.
[Mh] Termos MeSH primário: Vírus da Influenza A Subtipo H5N1/química
Vírus da Influenza A Subtipo H5N1/genética
RNA Replicase/química
RNA Replicase/genética
Proteínas de Ligação a RNA/química
Proteínas de Ligação a RNA/genética
Proteínas do Core Viral/química
Proteínas do Core Viral/genética
Proteínas Virais/química
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Ácido Aspártico/química
Genoma Viral
Células HEK293
Seres Humanos
Vírus da Influenza A Subtipo H5N1/fisiologia
Influenza Humana/virologia
Modelos Moleculares
Ressonância Magnética Nuclear Biomolecular
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Ressonância de Plasmônio de Superfície
Valina/química
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (NP protein, Influenza A virus); 0 (PB2 protein, Influenzavirus A); 0 (RNA-Binding Proteins); 0 (Recombinant Proteins); 0 (Viral Core Proteins); 0 (Viral Proteins); 30KYC7MIAI (Aspartic Acid); EC 2.7.7.48 (RNA Replicase); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191226


  4 / 20503 MEDLINE  
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[PMID]:27772535
[Au] Autor:Ljungberg M; Nilsson MKL; Melin K; Jönsson L; Carlsson A; Carlsson Å; Forssell-Aronsson E; Ivarsson T; Carlsson M; Starck G
[Ad] Endereço:1Department of Radiation Physics,Institute of Clinical Sciences,Sahlgrenska Academy,University of Gothenburg,Göteborg,Sweden.
[Ti] Título:1H magnetic resonance spectroscopy evidence for occipital involvement in treatment-naive paediatric obsessive-compulsive disorder.
[So] Source:Acta Neuropsychiatr;29(3):179-190, 2017 Jun.
[Is] ISSN:1601-5215
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Obsessive-compulsive disorder (OCD) is a chronic psychiatric disorder leading to considerable distress and disability. Therapies are effective in a majority of paediatric patients, however, many only get partial response. It is therefore important to study the underlying pathophysiology of the disorder. METHODS: 1H magnetic resonance spectroscopy (MRS) was used to study the concentration of brain metabolites in four different locations (cingulate gyrus and sulcus, occipital cortex, thalamus and right caudate nucleus). Treatment-naive children and adolescents with OCD (13 subjects) were compared with a group of healthy age- and gender-matched subjects (11 subjects). Multivariate analyses were performed on the concentration values. RESULTS: No separation between controls and patients was found. However, a correlation between metabolite concentrations and symptom severity as measured with the Children's Yale-Brown Obsessive-Compulsive Scale (CY-BOCS) was found. Strongest was the correlation with the CY-BOCS obsession subscore and aspartate and choline in the caudate nucleus (positively correlated with obsessions), lipids at 2 and 0.9 ppm in thalamus, and occipital glutamate+glutamine, N-acetylaspartate and myo-inosytol (negatively correlated with obsessions). CONCLUSIONS: The observed correlations between 1H MRS and CY-BOCS in treatment-naive patients further supports an occipital involvement in OCD. The results are consistent with our previous study on adult OCD patients. The 1H MRS data were not supportive of a separation between the patient and control groups.
[Mh] Termos MeSH primário: Encéfalo/diagnóstico por imagem
Transtorno Obsessivo-Compulsivo/diagnóstico por imagem
Transtorno Obsessivo-Compulsivo/metabolismo
Lobo Occipital/diagnóstico por imagem
Espectroscopia de Prótons por Ressonância Magnética/métodos
[Mh] Termos MeSH secundário: Adolescente
Ácido Aspártico/análogos & derivados
Ácido Aspártico/metabolismo
Encéfalo/metabolismo
Criança
Colina/metabolismo
Feminino
Seres Humanos
Inositol/metabolismo
Masculino
Transtorno Obsessivo-Compulsivo/tratamento farmacológico
Transtorno Obsessivo-Compulsivo/fisiopatologia
Lobo Occipital/metabolismo
Índice de Gravidade de Doença
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
30KYC7MIAI (Aspartic Acid); 4L6452S749 (Inositol); 997-55-7 (N-acetylaspartate); N91BDP6H0X (Choline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1017/neu.2016.52


  5 / 20503 MEDLINE  
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[PMID]:29305262
[Au] Autor:Ågren R; Sahlholm K; Nilsson J; Århem P
[Ad] Endereço:Department of Neuroscience, Retzius väg 8, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Electronic address: richard.agren@stud.ki.se.
[Ti] Título:Point mutation of a conserved aspartate, D69, in the muscarinic M receptor does not modify voltage-sensitive agonist potency.
[So] Source:Biochem Biophys Res Commun;496(1):101-104, 2018 01 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The muscarinic M receptor (M R) has been shown to display voltage-sensitive agonist binding, based on G protein-activated inward rectifier potassium channel (GIRK) opening and radioligand binding at different membrane voltages. A conserved aspartate in transmembrane segment (TM) II of M R, D69, has been proposed as the voltage sensor. While a recent paper instead presented evidence of tyrosines in TMs III, VI, and VII acting as voltage sensors, these authors were not able to record GIRK channel activation by a D69N mutant M R. In the present study, we succeeded in recording ACh-induced GIRK channel activation by this mutant at -80 and 0 mV. The acetylcholine EC was about 2.5-fold higher at 0 mV, a potency shift very similar to that observed at wild-type M R, indicating that voltage sensitivity persists at the D69N mutant. Thus, our present observations corroborate the notion that D69 is not responsible for voltage sensitivity of the M R.
[Mh] Termos MeSH primário: Acetilcolina/administração & dosagem
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Potenciais da Membrana/efeitos dos fármacos
Potenciais da Membrana/fisiologia
Receptor Muscarínico M2/genética
Receptor Muscarínico M2/metabolismo
[Mh] Termos MeSH secundário: Animais
Ácido Aspártico/genética
Células Cultivadas
Sequência Conservada
Relação Dose-Resposta a Droga
Mutagênese Sítio-Dirigida
Oócitos
Mutação Puntual/genética
Receptor Muscarínico M2/efeitos dos fármacos
Relação Estrutura-Atividade
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Receptor, Muscarinic M2); 30KYC7MIAI (Aspartic Acid); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  6 / 20503 MEDLINE  
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[PMID]:28993025
[Au] Autor:Cathey A; Ferguson KK; McElrath TF; Cantonwine DE; Pace G; Alshawabkeh A; Cordero JF; Meeker JD
[Ad] Endereço:Department of Environmental Health Sciences, University of Michigan School of Public Health, 1415 Washington Heights, Ann Arbor, MI 48109, USA.
[Ti] Título:Distribution and predictors of urinary polycyclic aromatic hydrocarbon metabolites in two pregnancy cohort studies.
[So] Source:Environ Pollut;232:556-562, 2018 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pregnant women and their fetuses represent susceptible populations to environmental contaminants. Exposure to polycyclic aromatic hydrocarbons (PAHs) among pregnant women may contribute to adverse birth outcomes such as preterm birth. Multiple previous studies have assessed airborne sources of PAHs among pregnant women but few have measured urinary PAH metabolites which can capture total exposure through multiple routes. The aim of this study was to bridge this knowledge gap by assessing longitudinal urinary PAH metabolite concentrations over two time points in pregnancy cohorts in Boston (N = 200) and Puerto Rico (N = 50) to better understand exposure distributions throughout pregnancy and how they relate to demographic factors. Urine samples were analyzed for 1-NAP, 2-NAP, 2-FLU, 1-PHE, 2,3-PHE, 4-PHE, 9-PHE, and 1-PYR. Concentrations of 2-NAP, 1-PYR, and 4-PHE were higher in Puerto Rico, while all other metabolites were present in higher concentrations in Boston. In Puerto Rico, intraclass correlation coefficients (ICC) were weak to moderate, ranging from 0.06 to 0.42. PAH metabolite concentrations were significantly higher among younger, heavier (except 1-NAP and 9-PHE), and less educated individuals in Boston only. Consistent significant associations between PAH concentrations and measured covariates were not found in Puerto Rico. Our results suggest that potentially important differences in PAH exposure exist between these two populations. Additionally, our results indicate that multiple urinary measurements are required to accurately assess PAH exposure throughout pregnancy.
[Mh] Termos MeSH primário: Poluentes Ambientais/urina
Exposição Materna/estatística & dados numéricos
Hidrocarbonetos Aromáticos Policíclicos/urina
[Mh] Termos MeSH secundário: Adulto
Ácido Aspártico/análogos & derivados
Ácido Aspártico/urina
Biomarcadores/urina
Boston
Estudos de Coortes
Monitoramento Ambiental
Feminino
Seres Humanos
Naftalenossulfonatos/urina
Hidrocarbonetos Aromáticos Policíclicos/análise
Gravidez
Pirróis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1-nitro-2-acetylpyrrole); 0 (Biomarkers); 0 (Environmental Pollutants); 0 (Naphthalenesulfonates); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Pyrroles); 141577-40-4 (N-(2-naphthalenesulfonyl)aspartyl-(2-phenethyl)amide); 30KYC7MIAI (Aspartic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE


  7 / 20503 MEDLINE  
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[PMID]:27775336
[Au] Autor:Zercher B; Hopkins TA
[Ad] Endereço:Department of Chemistry, Butler University , 4600 Sunset Avenue, Indianapolis, Indiana 46208, United States.
[Ti] Título:Induction of Circularly Polarized Luminescence from Europium by Amino Acid Based Ionic Liquids.
[So] Source:Inorg Chem;55(21):10899-10906, 2016 Nov 07.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Materials that emit circularly polarized light have application in several important industries. Because they show large optical activity and emit sharp visible light transitions, europium complexes are often exploited in applications that require circularly polarized luminescence (CPL). Chiral and coordinating ionic liquids based on prolinate, valinate, and aspartate anions are used to induce CPL from a simple achiral europium triflate salt. The sign of the induced CPL is dependent on the handedness (l vs d) of the amino acid anion. Comparison of the CPL spectra in ionic liquid with proline and valine vs aspartate shows that the number of carboxylate groups in the amino acid anion influences the europium coordination environment. DFT calculations predict a chiral eight-coordinate Eu(Pro) structure in the prolinate ionic liquid and a chiral seven- or eight-coordinate Eu(Asp) structure in the aspartate ionic liquid.
[Mh] Termos MeSH primário: Aminoácidos/química
Európio/química
Líquidos Iônicos/química
Substâncias Luminescentes/química
Mesilatos/química
[Mh] Termos MeSH secundário: Ácido Aspártico/química
Complexos de Coordenação/química
Luminescência
Medições Luminescentes
Modelos Moleculares
Prolina/química
Valina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Coordination Complexes); 0 (Ionic Liquids); 0 (Luminescent Agents); 0 (Mesylates); 30KYC7MIAI (Aspartic Acid); 444W947O8O (Europium); 9DLQ4CIU6V (Proline); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 20503 MEDLINE  
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[PMID]:29253022
[Au] Autor:Ha S; Kim I; Takata T; Kinouchi T; Isoyama M; Suzuki M; Fujii N
[Ad] Endereço:Graduate School of Science, Kyoto University, Kyoto, Japan.
[Ti] Título:Identification of á´…-amino acid-containing peptides in human serum.
[So] Source:PLoS One;12(12):e0189972, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biologically uncommon d-aspartate (d-Asp) residues have been shown to accumulate in proteins associated with age-related human disorders, such as cataract and Alzheimer disease. Such d-Asp-containing proteins are unlikely to be broken down completely because metabolic enzymes recognize only proteins or peptides composed exclusively of l-amino acids. Therefore, undigested d-Asp-containing peptides may exist in blood and, if detectable, may be a useful biomarker for associated diseases. In this study, we investigated d-amino acid-containing peptides in adult human serum by a qualitative d-amino acid analysis based on a diastereomer method and LC-MS/MS method. As a result, two d-Asp-containing peptides were detected in serum, both derived from the fibrinogen ß-chain, a glycoprotein that helps in the formation of blood clots. One of the peptides was fibrinopeptide B, which prevents fibrinogen from forming polymers of fibrin, and the other was same peptide with C-terminal Arginine missing. To our knowledge, this is the first report of the presence of d-amino acid-containing peptides in serum and the approach described will provide a new direction on the serum proteome and fragmentome.
[Mh] Termos MeSH primário: Aminoácidos/sangue
Peptídeos/sangue
[Mh] Termos MeSH secundário: Adulto
Doença de Alzheimer/sangue
Aminoácidos/química
Ácido Aspártico/metabolismo
Biomarcadores/sangue
Catarata/sangue
Cromatografia Líquida
Cristalinas/metabolismo
Fibrinogênio/metabolismo
Fibrinopeptídeo B/análise
Seres Humanos
Meia-Idade
Proteômica
Espectrometria de Massas em Tandem
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Biomarkers); 0 (Crystallins); 0 (Peptides); 30KYC7MIAI (Aspartic Acid); 36204-23-6 (Fibrinopeptide B); 9001-32-5 (Fibrinogen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189972


  9 / 20503 MEDLINE  
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[PMID]:28465627
[Au] Autor:Falzone M; Crespo E; Jones K; Khan G; Korn VL; Patel A; Patel M; Patel K; Perkins C; Siddiqui S; Stenger D; Yu E; Gelber M; Scheffler R; Nayda V; Ravin A; Komal R; Rudolf JD; Shen B; Gullo V; Demain AL
[Ad] Endereço:Research Institute of Scientists Emeriti (RISE), Charles A. Dana Research Institute, Drew University, Madison, NJ, USA.
[Ti] Título:Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of l-aspartic acid.
[So] Source:J Antibiot (Tokyo);70(7):828-831, 2017 Jul.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Streptomyces platensis MA7327 is a bacterium producing interesting antibiotics, which act by the novel mechanism of inhibiting fatty acid biosynthesis. The antibiotics produced by this actinomycete are platensimycin and platencin plus some minor related antibiotics. Platensimycin and platencin have activity against antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus; they also lack toxicity in animal models. Platensimycin also has activity against diabetes in a mouse model. We have been interested in studying the effects of primary metabolites on production of these antibiotics in our chemically defined production medium. In the present work, we tested 32 primary metabolites for their effect. They included 20 amino acids, 7 vitamins and 5 nucleic acid derivatives. Of these, only l-aspartic acid showed stimulation of antibiotic production. We conclude that the stimulatory effect of aspartic acid is due to its role as a precursor involved in the biosynthesis of aspartate-4-semialdehyde, which is the starting point for the biosynthesis of the 3-amino-2,4-dihydroxy benzoic acid portion of the platensimycin molecule.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Ácido Aspártico/administração & dosagem
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Adamantano/isolamento & purificação
Aminoácidos/administração & dosagem
Aminoácidos/metabolismo
Aminobenzoatos/isolamento & purificação
Aminofenóis/isolamento & purificação
Anilidas/isolamento & purificação
Antibacterianos/biossíntese
Ácido Aspártico/química
Ácidos Nucleicos/administração & dosagem
Ácidos Nucleicos/metabolismo
Compostos Policíclicos/isolamento & purificação
Vitaminas/administração & dosagem
Vitaminas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Aminobenzoates); 0 (Aminophenols); 0 (Anilides); 0 (Anti-Bacterial Agents); 0 (Nucleic Acids); 0 (Polycyclic Compounds); 0 (Vitamins); 0 (platencin); 30KYC7MIAI (Aspartic Acid); PJY633525U (Adamantane); Q3DQ78KOFY (platensimycin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.49


  10 / 20503 MEDLINE  
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Fotocópia
[PMID]:29215236
[Au] Autor:Vetrile LA; Zakharova IA; Kudrin VS; Klodt PM
[Ti] Título:[Effect of intranasally administered glutamate antibodies on the content of excitatory and inhibitory amino acids in the rat`s hippocampus and hypothalamus at the combined stress exposure].
[So] Source:Patol Fiziol Eksp Ter;60(1):4-10, 2016 Jan-Mar.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Objective. We studied the effect of glutamate antibodies by intranasal administration on the development of stress reactions and aspartate, glycine and taurine content in the brain structures of rats with different initial behavioral activity (active and passive). Methods. Stress caused by placing the animals in the living cell with water (21°Ð¡) covered with a grid for 30 min. Glutamate antibodies in a dose of 250 mg/kg in a volume of 10 mkl were administered intranasally to the experimental group of rats immediately after the stress. After 1 h after stress exposure and antibodies administration in all rats was investigated motor activity in the test of the «open field¼. Amino acids aspartate, glycine and taurine in the brain structures (hippocampus and hypothalamus) were determined by HPLC with fluorescence detection. Results. Combined water-immersion stress caused significant changes in the behavioral activity of rats in the «open field¼, but a more pronounced decline in the total index were observed in the behaviorally passive group of rats. The stress was accompanied by a change in the content of neurotransmitter amino acids (glycine and taurine) in the hippocampus. The most significant changes in the levels of glycine (decrease) and taurine (an increase) was observed in the hippocampus behaviorally active rats. Glutamate antibodies at a dose of 250 mg/kg administered intranasally immediately after stress exposure prevents the development of behavioral stress reactions and contributed to an increase in the hippocampus the content of glycine and taurine, related to stress-limiting systems. Conclusions. The glutamate antibodies under stress act as endogenous bioregulators and prevent the development of stress reactions.
[Mh] Termos MeSH primário: Anticorpos/farmacologia
Ácido Aspártico/metabolismo
Ácido Glutâmico
Glicina/metabolismo
Hipocampo/metabolismo
Estresse Psicológico/metabolismo
Taurina/metabolismo
[Mh] Termos MeSH secundário: Administração Intranasal
Animais
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 1EQV5MLY3D (Taurine); 30KYC7MIAI (Aspartic Acid); 3KX376GY7L (Glutamic Acid); TE7660XO1C (Glycine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE



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