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  1 / 21559 MEDLINE  
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[PMID]:29216450
[Au] Autor:Mateo Y; Johnson KA; Covey DP; Atwood BK; Wang HL; Zhang S; Gildish I; Cachope R; Bellocchio L; Guzmán M; Morales M; Cheer JF; Lovinger DM
[Ad] Endereço:Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, US National Institutes of Health, Rockville, MD, USA.
[Ti] Título:Endocannabinoid Actions on Cortical Terminals Orchestrate Local Modulation of Dopamine Release in the Nucleus Accumbens.
[So] Source:Neuron;96(5):1112-1126.e5, 2017 Dec 06.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dopamine (DA) transmission mediates numerous aspects of behavior. Although DA release is strongly linked to firing of DA neurons, recent developments indicate the importance of presynaptic modulation at striatal dopaminergic terminals. The endocannabinoid (eCB) system regulates DA release and is a canonical gatekeeper of goal-directed behavior. Here we report that extracellular DA increases induced by selective optogenetic activation of cholinergic neurons in the nucleus accumbens (NAc) are inhibited by CB1 agonists and eCBs. This modulation requires CB1 receptors on cortical glutamatergic afferents. Dopamine increases driven by optogenetic activation of prefrontal cortex (PFC) terminals in the NAc are similarly modulated by activation of these CB1 receptors. We further demonstrate that this same population of CB1 receptors modulates optical self-stimulation sustained by activation of PFC afferents in the NAc. These results establish local eCB actions on PFC terminals within the NAc that inhibit mesolimbic DA release and constrain reward-driven behavior.
[Mh] Termos MeSH primário: Dopamina/metabolismo
Endocanabinoides/farmacologia
Núcleo Accumbens/efeitos dos fármacos
Núcleo Accumbens/metabolismo
Córtex Pré-Frontal/efeitos dos fármacos
Terminações Pré-Sinápticas/efeitos dos fármacos
Terminações Pré-Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Fenômenos Eletrofisiológicos/efeitos dos fármacos
Espaço Extracelular/efeitos dos fármacos
Espaço Extracelular/metabolismo
Glutamatos/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Núcleo Accumbens/citologia
Córtex Pré-Frontal/citologia
Receptor CB1 de Canabinoide/agonistas
Recompensa
Autoestimulação
Transmissão Sináptica/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endocannabinoids); 0 (Glutamates); 0 (Receptor, Cannabinoid, CB1); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  2 / 21559 MEDLINE  
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[PMID]:28873095
[Au] Autor:Liu Y; Zhao N; Li C; Chang Q; Liu X; Liao Y; Pan R
[Ad] Endereço:Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China.
[Ti] Título:Longistyline C acts antidepressant in vivo and neuroprotection in vitro against glutamate-induced cytotoxicity by regulating NMDAR/NR2B-ERK pathway in PC12 cells.
[So] Source:PLoS One;12(9):e0183702, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Depressive disorder is a common psychiatric disease which ranks among the leading cause of disability worldwide. The antidepressants presently used had low cure rate and caused a variety of side-effects. The screening of antidepressant drugs is usually used classic behavioural tests and neuroprotective strategy. Longistyline C, a natural stilbene isolated from the leaves of Cajanuscajan (L.) Millsp, was firstly investigated the antidepressant effect using animal behavioural tests, and studied the neuroprotection and its possible signaling pathways on glutamate-induced injury in PC12 cells. The results of animal test demonstrated that longistyline C had the antidepressant activity, which the effect is similar to the positive control. In current study, we investigated the effect of longistyline C on glutamate-induced injury in PC12 cells and explored its possible signaling pathways. The results demonstrated that pretreatment with longistyline C at the concentrations of 2-8 µmol/L for 24 h had a significant reduction of the cytotoxicity induced by glutamate (15 mmol/L) in PC12 cells using MTT, lactate dehydrogenase (LDH) release assay and Annexin V-PI double staining. Subsequently, we found that pretreatment with longistyline C (8 µmol/L) could drastically down-regulate the over-expression of NMDAR/NR2B and Ca2+/calmodulin-dependent protein kinase II (CaMKII), up-regulate the expressions of p-ERK and p-CREB and alleviate ER stress. In conclusison, longistyline C is most possibly through regulating NMDAR/NR2B-ERK1/2 related pathway and restoring endoplasmic reticulum function to exert neuroprotective effect against glutamate-induced injury in PC12 cells.
[Mh] Termos MeSH primário: Antidepressivos/farmacologia
Glutamatos/efeitos adversos
Receptores de N-Metil-D-Aspartato/metabolismo
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Comportamento Animal
Cálcio/metabolismo
Caspase 12/metabolismo
Caspase 9/metabolismo
Sobrevivência Celular
Teste de Esforço
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Proteínas de Choque Térmico/metabolismo
L-Lactato Desidrogenase/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos ICR
Neuroproteção
Células PC12
Ratos
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais
Natação
Fator de Transcrição CHOP/metabolismo
Proteína 1 de Ligação a X-Box/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidepressive Agents); 0 (Ddit3 protein, mouse); 0 (Glutamates); 0 (Heat-Shock Proteins); 0 (NR2B NMDA receptor); 0 (Reactive Oxygen Species); 0 (Receptors, N-Methyl-D-Aspartate); 0 (Stilbenes); 0 (X-Box Binding Protein 1); 0 (Xbp1 protein, mouse); 0 (longistyline C); 0 (molecular chaperone GRP78); 147336-12-7 (Transcription Factor CHOP); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.4.22.- (Caspase 12); EC 3.4.22.- (Caspase 9); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183702


  3 / 21559 MEDLINE  
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[PMID]:28807780
[Au] Autor:Jeong H; Paik YK
[Ad] Endereço:Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea; Department of Integrative Omics for Biomedical Science, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
[Ti] Título:MGL-1 on AIY neurons translates starvation to reproductive plasticity via neuropeptide signaling in Caenorhabditis elegans.
[So] Source:Dev Biol;430(1):80-89, 2017 10 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reproductive plasticity is the ability of an animal to modulate its reproductive functions in response to environmental changes. For example, Caenorhabditis elegans, a free-living nematode, can adjust the onsets of oogenesis and embryogenesis under harsh environmental conditions, including starvation. However, the molecular mechanisms used to perceive and translate environmental signals into reproductive functional adjustments remain largely uncharacterized. We discovered that in C. elegans, the glutamate receptor homolog MGL-1 initiated reproductive plasticity in response to starvation. A genetic analysis of the mutant gene, mgl-1(tm1811), suggested that starvation delayed the onsets of oogenesis and embryogenesis via MGL-1. Cell-specific rescues of mgl-1 deletion mutants, which used transgenic lines designed to express MGL-1 in different neurons (e.g., RMD, AIA, AIY, and NSM), suggested that only AIY-rescued animals exhibited normal delays in oogenesis and embryogenesis equivalent to those of wild-type animals, suggesting recovery. Furthermore, in AIY neurons, MGL-1 appears to use neuropeptide signaling, rather than glutamate, to translate starvation stimuli into delayed oogenesis and embryogenesis. Our findings, which reveal molecular linkages between starvation signals and reproductive alterations, may provide a basis for understanding energy reallocation mechanisms, as the mgl-1 deletion mutant exhibited more severe reductions in lifespan and fat accumulation than did wild-type animals under starvation conditions. Taken together, MGL-1 is the molecular driver underlying the translation of starvation signals to reproduction plasticity in an AIY neuron-specific manner.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/citologia
Caenorhabditis elegans/metabolismo
Neurônios/metabolismo
Neuropeptídeos/metabolismo
Transdução de Sinais
Inanição/metabolismo
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/embriologia
Embrião não Mamífero/citologia
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Deleção de Genes
Glutamatos/metabolismo
Neurônios/citologia
Oogênese
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Flp-1 protein, C elegans); 0 (Glutamates); 0 (Neuropeptides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  4 / 21559 MEDLINE  
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[PMID]:28796499
[Au] Autor:Cheng S; Fu X; Wang X; Liao Y; Zeng L; Dong F; Yang Z
[Ad] Endereço:Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences , Xingke Road 723, Tianhe District, Guangzhou 510650, China.
[Ti] Título:Studies on the Biochemical Formation Pathway of the Amino Acid l-Theanine in Tea (Camellia sinensis) and Other Plants.
[So] Source:J Agric Food Chem;65(33):7210-7216, 2017 Aug 23.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tea (Camellia sinensis) is the most widely consumed beverage aside from water. The flavor of tea is conferred by certain metabolites, especially l-theanine, in C. sinensis. To determine why more l-theanine accumulates in C. sinensis than in other plants, we compare l-theanine contents between C. sinensis and other plant species (Camellia nitidissima, Camellia japonica, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum) and use a stable isotope labeling approach to elucidate its biosynthetic route. We quantify relevant intermediates and metabolites by mass spectrometry. l-Glutamic acid, a precursor of l-theanine, is present in most plants, while ethylamine, another precursor of l-theanine, specifically accumulates in Camellia species, especially C. sinensis. Most plants contain the enzyme/gene catalyzing the conversion of ethylamine and l-glutamic acid to l-theanine. After supplementation with [ H ]ethylamine, all the plants produce [ H ]l-theanine, which suggests that ethylamine availability is the reason for the difference in l-theanine accumulation between C. sinensis and other plants.
[Mh] Termos MeSH primário: Camellia sinensis/metabolismo
Glutamatos/biossíntese
[Mh] Termos MeSH secundário: Amida Sintases/genética
Amida Sintases/metabolismo
Vias Biossintéticas
Camellia sinensis/enzimologia
Camellia sinensis/genética
Glutamato-Amônia Ligase/genética
Glutamato-Amônia Ligase/metabolismo
Ácido Glutâmico/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glutamates); 0 (Plant Proteins); 3KX376GY7L (Glutamic Acid); 8021PR16QO (theanine); EC 6.3.1.- (Amide Synthases); EC 6.3.1.2 (Glutamate-Ammonia Ligase); EC 6.3.1.6 (theanine synthetase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02437


  5 / 21559 MEDLINE  
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[PMID]:28738503
[Au] Autor:Gajaria TK; Suthar P; Baghel RS; Balar NB; Sharnagat P; Mantri VA; Reddy CRK
[Ad] Endereço:Division of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
[Ti] Título:Integration of protein extraction with a stream of byproducts from marine macroalgae: A model forms the basis for marine bioeconomy.
[So] Source:Bioresour Technol;243:867-873, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study describes an advanced biorefinery model for marine macroalgae that assumes significant importance in the context of marine bio-economy. The method investigated in this study integrates the extraction of crude proteins with recovery of minerals rich sap, lipids, ulvan and cellulose from fresh biomass of Ulva lactuca. The protein content extracted was 11±2.12% on dry weight basis with recovery efficiency of 68.75±4.01%. The amino acid composition of crude protein fraction showed iso-leucine as the most abundant amino acid with 16.51±0.03% followed by histidine, arginine, tyrosine, serine, aspartic acid, threonine, phenyl alanine, leucine, alanine, lysine, glycine and glutamic acid (0.22±0.24%). The digestibility of protein was as high as 85.86±5.92% indicating its suitability for use in food supplements. The protein production with co-recovery of other products would not only result in effective utilisation marine macroalgal resources but also forms the basis for marine bio-economy.
[Mh] Termos MeSH primário: Microalgas
Proteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Aminoácidos
Glutamatos
Alga Marinha
Treonina
Valina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Glutamates); 0 (Proteins); 2ZD004190S (Threonine); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  6 / 21559 MEDLINE  
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[PMID]:28674172
[Au] Autor:Tang S; Wang IJ; Yue C; Takano H; Terzic B; Pance K; Lee JY; Cui Y; Coulter DA; Zhou Z
[Ad] Endereço:Department of Genetics and.
[Ti] Título:Loss of CDKL5 in Glutamatergic Neurons Disrupts Hippocampal Microcircuitry and Leads to Memory Impairment in Mice.
[So] Source:J Neurosci;37(31):7420-7437, 2017 Aug 02.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclin-dependent kinase-like 5 (CDKL5) deficiency is a neurodevelopmental disorder characterized by epileptic seizures, severe intellectual disability, and autistic features. Mice lacking CDKL5 display multiple behavioral abnormalities reminiscent of the disorder, but the cellular origins of these phenotypes remain unclear. Here, we find that ablating CDKL5 expression specifically from forebrain glutamatergic neurons impairs hippocampal-dependent memory in male conditional knock-out mice. Hippocampal pyramidal neurons lacking CDKL5 show decreased dendritic complexity but a trend toward increased spine density. This morphological change is accompanied by an increase in the frequency of spontaneous miniature EPSCs and interestingly, miniature IPSCs. Using voltage-sensitive dye imaging to interrogate the evoked response of the CA1 microcircuit, we find that CA1 pyramidal neurons lacking CDKL5 show hyperexcitability in their dendritic domain that is constrained by elevated inhibition in a spatially and temporally distinct manner. These results suggest a novel role for CDKL5 in the regulation of synaptic function and uncover an intriguing microcircuit mechanism underlying impaired learning and memory. Cyclin-dependent kinase-like 5 (CDKL5) deficiency is a severe neurodevelopmental disorder caused by mutations in the gene. Although constitutive knock-out mice have recapitulated key aspects of human symptomatology, the cellular origins of CDKL5 deficiency-related phenotypes are unknown. Here, using conditional knock-out mice, we show that hippocampal-dependent learning and memory deficits in CDKL5 deficiency have origins in glutamatergic neurons of the forebrain and that loss of CDKL5 results in the enhancement of synaptic transmission and disruptions in neural circuit dynamics in a spatially and temporally specific manner. Our findings demonstrate that CDKL5 is an important regulator of synaptic function in glutamatergic neurons and serves a critical role in learning and memory.
[Mh] Termos MeSH primário: Glutamatos/metabolismo
Hipocampo/fisiopatologia
Transtornos da Memória/fisiopatologia
Rede Nervosa/fisiopatologia
Neurônios/metabolismo
Proteínas Serina-Treonina Quinases/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
Memória
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Proteínas Serina-Treonina Quinases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glutamates); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.22 (CDKL5 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.0539-17.2017


  7 / 21559 MEDLINE  
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[PMID]:28640602
[Au] Autor:Ye C; Zeng X; Zhu J; Liu Y; Ye Q; Qiao S; Zeng X
[Ad] Endereço:State Key Laboratory of Animal Nutrition, China Agricultural University , Beijing 100193, People's Republic of China.
[Ti] Título:Dietary N-Carbamylglutamate Supplementation in a Reduced Protein Diet Affects Carcass Traits and the Profile of Muscle Amino Acids and Fatty Acids in Finishing Pigs.
[So] Source:J Agric Food Chem;65(28):5751-5758, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate whether dietary N-carbamylglutamate (NCG) supplementation in a reduced protein diet affected carcass traits and meat quality in finishing pigs. A total of 120 gilts were randomly assigned to one of four treatments for 40 days, including a standard protein diet (SP), a reduced protein diet supplemented with 1.7% l-alanine (RP + Ala), a reduced protein diet supplemented with 1.0% l-arginine (RP + Arg), and a reduced protein diet supplemented with 0.1% NCG and 1.7% l-alanine (RP + NCG). NCG supplementation increased the endogenous synthesis of l-arginine. The RP + NCG diet significantly increased the loin eye area (p < 0.05) and tended to decrease the 10th rib fat depth (p = 0.08). NCG supplementation in a reduced protein diet was effective to produce functional pork with a high content of leucine (p < 0.05). The composition of several ω-6 and ω-3 polyunsaturated fatty acids (PUFAs) but not the ratio of ω-6/ω-3 PUFAs in muscles was altered in finishing pigs with dietary NCG supplementation. In conclusion, the RP + NCG diet is effective to increase the longissimus dorsi muscle area, decrease back fat accretion, and produce functional pork with a high content of leucine but without a negative impact on the muscle fatty acid profile in finishing pigs.
[Mh] Termos MeSH primário: Aminoácidos/metabolismo
Ração Animal/análise
Dieta com Restrição de Proteínas/veterinária
Suplementos Nutricionais/análise
Glutamatos/administração & dosagem
Carne/análise
Músculos/metabolismo
Suínos/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/química
Animais
Arginina/metabolismo
Glutamatos/metabolismo
Leucina/metabolismo
Músculos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Glutamates); 1188-38-1 (N-carbamylglutamate); 94ZLA3W45F (Arginine); GMW67QNF9C (Leucine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02301


  8 / 21559 MEDLINE  
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[PMID]:28554889
[Au] Autor:Siu CR; Beshara SP; Jones DG; Murphy KM
[Ad] Endereço:McMaster Integrative Neuroscience Discovery and Study Program, McMaster University, Hamilton, Ontario L8S 4K1, Canada.
[Ti] Título:Development of Glutamatergic Proteins in Human Visual Cortex across the Lifespan.
[So] Source:J Neurosci;37(25):6031-6042, 2017 Jun 21.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditionally, human primary visual cortex (V1) has been thought to mature within the first few years of life, based on anatomical studies of synapse formation, and establishment of intracortical and intercortical connections. Human vision, however, develops well beyond the first few years. Previously, we found prolonged development of some GABAergic proteins in human V1 (Pinto et al., 2010). Yet as >80% of synapses in V1 are excitatory, it remains unanswered whether the majority of synapses regulating experience-dependent plasticity and receptive field properties develop late, like their inhibitory counterparts. To address this question, we used Western blotting of postmortem tissue from human V1 (12 female, 18 male) covering a range of ages. Then we quantified a set of postsynaptic glutamatergic proteins (PSD-95, GluA2, GluN1, GluN2A, GluN2B), calculated indices for functional pairs that are developmentally regulated (GluA2:GluN1; GluN2A:GluN2B), and determined interindividual variability. We found early loss of GluN1, prolonged development of PSD-95 and GluA2 into late childhood, protracted development of GluN2A until ∼40 years, and dramatic loss of GluN2A in aging. The GluA2:GluN1 index switched at ∼1 year, but the GluN2A:GluN2B index continued to shift until ∼40 year before changing back to GluN2B in aging. We also identified young childhood as a stage of heightened interindividual variability. The changes show that human V1 develops gradually through a series of five orchestrated stages, making it likely that V1 participates in visual development and plasticity across the lifespan. Anatomical structure of human V1 appears to mature early, but vision changes across the lifespan. This discrepancy has fostered two hypotheses: either other aspects of V1 continue changing, or later changes in visual perception depend on extrastriate areas. Previously, we showed that some GABAergic synaptic proteins change across the lifespan, but most synapses in V1 are excitatory leaving unanswered how they change. So we studied expression of glutamatergic proteins in human V1 to determine their development. Here we report prolonged maturation of glutamatergic proteins, with five stages that map onto life-long changes in human visual perception. Thus, the apparent discrepancy between development of structure and function may be explained by life-long synaptic changes in human V1.
[Mh] Termos MeSH primário: Glutamatos/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Córtex Visual/crescimento & desenvolvimento
Córtex Visual/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Envelhecimento/fisiologia
Criança
Pré-Escolar
Proteína 4 Homóloga a Disks-Large
Feminino
Seres Humanos
Lactente
Recém-Nascido
Peptídeos e Proteínas de Sinalização Intracelular
Masculino
Proteínas de Membrana
Meia-Idade
Rede Nervosa/crescimento & desenvolvimento
Rede Nervosa/metabolismo
Plasticidade Neuronal/fisiologia
Receptores de Glutamato/metabolismo
Sinapses/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DLG4 protein, human); 0 (Disks Large Homolog 4 Protein); 0 (Glutamates); 0 (Intracellular Signaling Peptides and Proteins); 0 (Membrane Proteins); 0 (Nerve Tissue Proteins); 0 (Receptors, Glutamate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.2304-16.2017


  9 / 21559 MEDLINE  
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[PMID]:28434875
[Au] Autor:Martín-Gago P; Fansa EK; Winzker M; Murarka S; Janning P; Schultz-Fademrecht C; Baumann M; Wittinghofer A; Waldmann H
[Ad] Endereço:Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn Straße 11, 44227 Dortmund, Germany.
[Ti] Título:Covalent Protein Labeling at Glutamic Acids.
[So] Source:Cell Chem Biol;24(5):589-597.e5, 2017 May 18.
[Is] ISSN:2451-9456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Covalent labeling of amino acids in proteins by reactive small molecules, in particular at cysteine SH and lysine NH groups, is a powerful approach to identify and characterize proteins and their functions. However, for the less-reactive carboxylic acids present in Asp and Glu, hardly any methodology is available. Employing the lipoprotein binding chaperone PDE6δ as an example, we demonstrate that incorporation of isoxazolium salts that resemble the structure and reactivity of Woodward's reagent K into protein ligands provides a novel method for selective covalent targeting of binding site carboxylic acids in whole proteomes. Covalent adduct formation occurs via rapid formation of enol esters and the covalent bond is stable even in the presence of strong nucleophiles. This new method promises to open up hitherto unexplored opportunities for chemical biology research.
[Mh] Termos MeSH primário: Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/química
Glutamatos/química
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/metabolismo
Ligantes
Modelos Moleculares
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glutamates); 0 (Ligands); EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 6)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


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[PMID]:28429900
[Au] Autor:Wang D; Gao Q; Wang T; Qian F; Wang Y
[Ad] Endereço:State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science and Technology, Anhui Agricultural University, Anhui, PR China.
[Ti] Título:Theanine: the unique amino acid in the tea plant as an oral hepatoprotective agent.
[So] Source:Asia Pac J Clin Nutr;26(3):384-391, 2017 May.
[Is] ISSN:0964-7058
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:For thousands of years, humans have consumed tea made from leaves of Camellia sinensis, first as a medicinal herb and then as a widely popular beverage. In the past 10 years, theanine, a tea-derived, unique, nonproteinic amino acid, has been extensively studied for its health benefits. Recently, multiple lines of evidence have proven its beneficial effects on hepatic and immune functions. One possible mechanism for its biological activity involves the downregulation of the inflammatory response through the induction of nitric oxide production and glutathione synthesis. In this review, we summarize published results describing the potential mechanisms for these beneficial health effects and provide new insight into how theanine can be therapeutic for liver injury and chronic liver disease.
[Mh] Termos MeSH primário: Camellia sinensis/química
Glutamatos/administração & dosagem
Hepatopatias/prevenção & controle
Extratos Vegetais/administração & dosagem
Chá/química
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Glutamatos/farmacocinética
Glutamatos/uso terapêutico
Promoção da Saúde
Seres Humanos
Fatores Imunológicos
Extratos Vegetais/uso terapêutico
Folhas de Planta
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Glutamates); 0 (Immunologic Factors); 0 (Plant Extracts); 0 (Tea); 8021PR16QO (theanine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.6133/apjcn.032017.11



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