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  1 / 26808 MEDLINE  
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[PMID]:29309428
[Au] Autor:Sarika K; Hossain F; Muthusamy V; Zunjare RU; Baveja A; Goswami R; Thirunavukkarasu N; Jha SK; Gupta HS
[Ad] Endereço:Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
[Ti] Título:Opaque16, a high lysine and tryptophan mutant, does not influence the key physico-biochemical characteristics in maize kernel.
[So] Source:PLoS One;13(1):e0190945, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The enhancement of lysine and tryptophan in maize is so far basedon opaque2(o2) mutant, that along with the endosperm-modifiersled to development of Quality Protein Maize[QPM]. Though many mutants improving the endospermic protein quality were discovered, they could not be successfully deployed. Recently discovered opaque16 (o16)mutant enhances the lysine and tryptophan content in maize endosperm. In the present study, the influence of o16 on the endosperm modification was analyzed in four F2 populations, two each segregating for o16 allele alone and in combination with o2. The recessive o16o16 seed endosperm was found to be vitreousphenotypically similar to wild-O16O16. The mutant did not influence the degree of kernel opaqueness in o2o2 genetic background as opaqueness in o2o2/O16O16 and o2o2/o16o16 was similar. Grain hardness of o16o16 was comparable with the normal and QPM maize. The pattern of microscopic organization of proteinaceous matrix and starch granules, and zein profiling of the storage protein in o16o16 were found to be similar with normal maize endosperm, but distinct from the o2o2-soft genotype. The pattern in o2o2/o16o16 was unique and different from o2o2 and o16o16 as well. Here we demonstrated the effects of o16 on physico-biochemical characteristics of endosperm and report of o16 possessing negligible influence on kernel modification and hardness, which holds a great significance in maize quality breeding programme.
[Mh] Termos MeSH primário: Lisina/metabolismo
Mutação
Proteínas de Plantas/metabolismo
Triptofano/metabolismo
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Genes de Plantas
Marcadores Genéticos
Proteínas de Plantas/química
Proteínas de Plantas/genética
Zea mays/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Plant Proteins); 8DUH1N11BX (Tryptophan); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190945


  2 / 26808 MEDLINE  
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[PMID]:28461065
[Au] Autor:Hu D; Bowder D; Wei W; Thompson J; Wilson MA; Xiang SH
[Ad] Endereço:Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, United States; School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, United States.
[Ti] Título:Tryptophan 375 stabilizes the outer-domain core of gp120 for HIV vaccine immunogen design.
[So] Source:Vaccine;35(23):3067-3075, 2017 05 25.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The outer-domain core of gp120 may serve as a better HIV vaccine immunogen than the full-length gp120 because of its greater stability and immunogenicity. In our previous report, we introduced two disulfide bonds to the outer-domain core of gp120 to fix its conformation into a CD4-bound state, which resulted in a significant increase in its immunogenicity when compared to the wild-type outer-domain core. In this report, to further improve the immunogenicity of the outer-domain core based immunogen, we have introduced a Tryptophan residue at gp120 amino acid sequence position 375 (375S/W). Our data from immunized guinea pigs indeed shows a striking increase in the immune response due to this stabilized core outer-domain. Therefore, we conclude that the addition of 375W to the outer-domain core of gp120 further stabilizes the structure of immunogen and increases the immunogenicity.
[Mh] Termos MeSH primário: Vacinas contra a AIDS/imunologia
Anticorpos Anti-HIV/imunologia
Proteína gp120 do Envelope de HIV/química
Proteína gp120 do Envelope de HIV/imunologia
Imunogenicidade da Vacina
Triptofano/química
[Mh] Termos MeSH secundário: Vacinas contra a AIDS/administração & dosagem
Vacinas contra a AIDS/química
Substituição de Aminoácidos
Animais
Anticorpos Neutralizantes/imunologia
Antígenos CD4
Desenho de Drogas
Epitopos/química
Cobaias
Anticorpos Anti-HIV/sangue
HIV-1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AIDS Vaccines); 0 (Antibodies, Neutralizing); 0 (CD4 Antigens); 0 (Epitopes); 0 (HIV Antibodies); 0 (HIV Envelope Protein gp120); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29177308
[Au] Autor:Fang K; Wu S; Dong G; Wu Y; Chen S; Liu J; Wang W; Sheng C
[Ad] Endereço:School of Pharmacy, East China University of Science and Technology, Shanghai 200237, P. R. China. wwang@unm.edu.
[Ti] Título:Discovery of IDO1 and DNA dual targeting antitumor agents.
[So] Source:Org Biomol Chem;15(47):9992-9995, 2017 Dec 06.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The development of small molecules for cancer immunotherapy is highly challenging and indoleamine 2,3-dioxygenase 1 (IDO1) represents a promising target. Inspired by the synergistic effects between IDO1 inhibitors and traditional antitumor chemotherapeutics, the first orally active dual IDO1 and DNA targeting agents were designed by the pharmacophore fusion strategy. The bifunctional hybrids exhibited enhanced IDO1 enzyme inhibitory activity and in vitro cytotoxicity as compared to IDO1 inhibitor 1-methyl-tryptophan and DNA alkylating agent melphalan. In a murine LLC tumor model, the dual targeting agents demonstrated excellent antitumor efficacy, highlighting the advantages of this novel design strategy to improve the efficacy of small molecule cancer immunotherapy.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
DNA de Neoplasias/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Indolamina-Pirrol 2,3,-Dioxigenase/antagonistas & inibidores
Melfalan/farmacologia
Triptofano/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/síntese química
Antineoplásicos/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Descoberta de Drogas
Ensaios de Seleção de Medicamentos Antitumorais
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Seres Humanos
Imunoterapia
Indolamina-Pirrol 2,3,-Dioxigenase/metabolismo
Melfalan/síntese química
Melfalan/química
Camundongos
Estrutura Molecular
Neoplasias Experimentais/tratamento farmacológico
Relação Estrutura-Atividade
Triptofano/síntese química
Triptofano/química
Triptofano/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1-methyltryptophan); 0 (Antineoplastic Agents); 0 (DNA, Neoplasm); 0 (Enzyme Inhibitors); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (indoleamine 2,3-dioxygenase 1, human); 8DUH1N11BX (Tryptophan); Q41OR9510P (Melphalan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob02529g


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[PMID]:29396377
[Au] Autor:Anderson GM; Eighmie JT
[Ad] Endereço:Yale Child Study Center, Yale School of Medicine, 230 South Frontage Rd., New Haven, CT, USA; Department of Laboratory Medicine, Yale School of Medicine, 230 South Frontage Rd., New Haven, CT, USA. Electronic address: george.anderson@yale.edu.
[Ti] Título:The determination of 5-methoxytryptophan in human plasma.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:124-128, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Methoxyindoles have been of continued interest due to their biological effects. Recent studies using enzyme-linked immunosorbent assays (ELISAs) have reported micromolar levels of 5-methoxytryptophan (MeOTRP) in human plasma. This prompted our development of a method for the determination of MeOTRP in human plasma that involved solid phase extraction (SPE) followed by HPLC. The identity of a putative MeOTRP peak was confirmed through chromatographic, voltammometric and fluorometric characterization. Using a standard addition approach, actual mean human plasma MeOTRP levels were found to be approximately 2ng/mL, 20 to 100 times lower than previously reported. Prior studies on the physiological and possible biomarker roles of MeOTRP need to be reconsidered and future studies of MeOTRP need to take the present findings into consideration.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Extração em Fase Sólida/métodos
Triptofano/análogos & derivados
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Limite de Detecção
Masculino
Meia-Idade
Reprodutibilidade dos Testes
Triptofano/sangue
Triptofano/química
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
2504-22-5 (5-methoxytryptophan); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


  5 / 26808 MEDLINE  
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[PMID]:28454699
[Au] Autor:Murthi P; Wallace EM; Walker DW
[Ad] Endereço:Department of Medicine, School of Clinical Sciences, Monash University, Monash Medical Centre, Clayton, Victoria, 3168, Australia; The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright St., Clayton, Victoria, 3168, Australia.
[Ti] Título:Altered placental tryptophan metabolic pathway in human fetal growth restriction.
[So] Source:Placenta;52:62-70, 2017 Apr.
[Is] ISSN:1532-3102
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Tryptophan is a substrate for kynurenine pathway metabolism in the placenta. We investigated if kynurenine metabolites change over gestation, if they are different between pregnancies with normal and fetal growth restriction (FGR), and if the oxygen environment modulated kynurenine pathway activity in the human placenta. METHODS: Tryptophan, kynurenine, and downstream kynurenine metabolites were determined in maternal venous blood, umbilical cord blood, and placental samples obtained in 1st and 3rd trimester pregnancies including FGR, and in the media of placental explants incubated with 20% or 5-8% O for 24, 48 or 72 h. RESULTS: All the major kynurenine metabolites were present in cord blood, and in general were higher than in maternal blood. IDO and TDO mRNA and protein expression, responsible for kynurenine production from tryptophan, were significantly lower in placentas from FGR pregnancies compared with control. Explants prepared from 1st and 3rd trimester placentas actively produced all the major kynurenine pathway metabolites which, together with expression of IDO, TDO, KYN-OHase and 3HAO mRNAs, were significantly lower after 24 h exposure to 5-8% O compared to 20% O CONCLUSIONS: Expression and activity of the kynurenine pathway is present in the placenta from early gestation, and is down-regulated by hypoxia and in FGR pregnancies.
[Mh] Termos MeSH primário: Retardo do Crescimento Fetal/metabolismo
Redes e Vias Metabólicas/fisiologia
Placenta/metabolismo
Triptofano/metabolismo
[Mh] Termos MeSH secundário: Adulto
Feminino
Sangue Fetal/metabolismo
Seres Humanos
Cinurenina/metabolismo
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
343-65-7 (Kynurenine); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:27778165
[Au] Autor:Bai M; Liu H; Xu K; Oso AO; Wu X; Liu G; Tossou MC; Al-Dhabi NA; Duraipandiyan V; Xi Q; Yin Y
[Ad] Endereço:Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, 410125, Hunan, China.
[Ti] Título:A review of the immunomodulatory role of dietary tryptophan in livestock and poultry.
[So] Source:Amino Acids;49(1):67-74, 2017 01.
[Is] ISSN:1438-2199
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Tryptophan, a nutritionally essential amino acid, is active in the regulation of immune responses in animals. The products of tryptophan metabolism, such as indoleamine 2,3-dioxygenase, kynurenine, quinolinic acid, and melatonin, may improve immunity in an organism and induce anti-inflammatory responses. The immune tolerance processes mediated by tryptophan metabolites are not well understood. Recent studies have reported that the enzymes that break down tryptophan through the kynurenine metabolic pathway are found in numerous cell types, including immunocytes. Moreover, some tryptophan metabolites have been shown to play a role in the inhibition of T lymphocyte proliferation, elevation of immunoglobulin levels in the blood, and promotion of antigen-presenting organization in tissues. This review summarizes the effects and mechanisms of tryptophan and metabolites in immune functions in livestock and poultry. It also highlights the areas in which our understanding of the role(s) of tryptophan is incomplete and suggests possible future research that might prove of benefit to livestock and poultry producers.
[Mh] Termos MeSH primário: Suplementos Nutricionais
Imunomodulação/efeitos dos fármacos
Indolamina-Pirrol 2,3,-Dioxigenase/imunologia
Linfócitos/efeitos dos fármacos
Triptofano/imunologia
[Mh] Termos MeSH secundário: Ração Animal
Animais
Seres Humanos
Imunidade Inata
Indolamina-Pirrol 2,3,-Dioxigenase/metabolismo
Cinurenina/imunologia
Cinurenina/metabolismo
Gado
Linfócitos/citologia
Linfócitos/imunologia
Melatonina/imunologia
Melatonina/metabolismo
Aves Domésticas/imunologia
Ácido Quinolínico/imunologia
Ácido Quinolínico/metabolismo
Serotonina/imunologia
Serotonina/metabolismo
Suínos/imunologia
Triptofano/administração & dosagem
Triptofano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 333DO1RDJY (Serotonin); 343-65-7 (Kynurenine); 8DUH1N11BX (Tryptophan); F6F0HK1URN (Quinolinic Acid); JL5DK93RCL (Melatonin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1007/s00726-016-2351-8


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[PMID]:29247504
[Au] Autor:Gallegos-Tabanico A; Sarabia-Sainz JA; Sarabia-Sainz HM; Carrillo Torres R; Guzman-Partida AM; Monfort GR; Silva-Campa E; Burgara-Estrella AJ; Angulo-Molina A; Acosta-Elias M; Pedroza-Montero M; Vazquez-Moreno L
[Ad] Endereço:Departamento de Física, Universidad de Sonora, Hermosillo, Sonora, 83000, México.
[Ti] Título:Molecular recognition of glyconanoparticles by RCA and E. coli K88 - designing transports for targeted therapy.
[So] Source:Acta Biochim Pol;64(4):671-677, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The targeted drug delivery has been studied as one of the main methods in medicine to ensure successful treatments of diseases. Pharmaceutical sciences are using micro or nano carriers to obtain a controlled delivery of drugs, able to selectively interact with pathogens, cells or tissues. In this work, we modified bovine serum albumin (BSA) with lactose, obtaining a neoglycan (BSA-Lac). Subsequently, we synthesized glyconanoparticles (NPBSA-Lac) with the premise that it would be recognized by microbial galactose specific lectins. NPBSA-Lac were tested for bio-recognition with adhesins of E. coli K88 and Ricinus communis agglutinin I (RCA). Glycation of BSA with lactose was analyzed by electrophoresis, infrared spectroscopy and fluorescence. Approximately 41 lactoses per BSA molecule were estimated. Nanoparticles were obtained using water in oil emulsion method and spheroid morphology with a range size of 300-500 nm was observed. Specific recognition of NPBSA-Lac by RCA and E. coli K88 was displayed by aggregation of nanoparticles analyzed by dynamic light scattering and atomic force microscopy. The results indicate that the lactosylated nanovectors could be targeted at the E. coli K88 adhesin and potentially could be used as a transporter for an antibacterial drug.
[Mh] Termos MeSH primário: Antígenos de Bactérias/metabolismo
Portadores de Fármacos/metabolismo
Proteínas de Escherichia coli/metabolismo
Proteínas de Fímbrias/metabolismo
Nanopartículas/química
Lectinas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Portadores de Fármacos/química
Eletroforese em Gel de Poliacrilamida
Escherichia coli/metabolismo
Lactose/química
Microscopia de Força Atômica
Peso Molecular
Tamanho da Partícula
Soroalbumina Bovina/química
Espectrofotometria Infravermelho
Espectroscopia de Infravermelho com Transformada de Fourier
Triptofano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Drug Carriers); 0 (Escherichia coli Proteins); 0 (K88 antigen, E coli); 0 (Plant Lectins); 0 (Ricinus communis agglutinin-1); 147680-16-8 (Fimbriae Proteins); 27432CM55Q (Serum Albumin, Bovine); 8DUH1N11BX (Tryptophan); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2017_1639


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[PMID]:29294327
[Au] Autor:Ren C; Liu J; Zhou J; Liang H; Wang Y; Sun Y; Ma B; Yin Y
[Ad] Endereço:Departments of Human Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
[Ti] Título:Low levels of serum serotonin and amino acids identified in migraine patients.
[So] Source:Biochem Biophys Res Commun;496(2):267-273, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Migraine is a highly disabling primary headache associated with a high socioeconomic burden and a generally high prevalence. The clinical management of migraine remains a challenge. This study was undertaken to identify potential serum biomarkers of migraine. Using Liquid Chromatography coupled to Mass Spectrometry (LC-MS), the metabolomic profile of migraine was compared with healthy individuals. Principal component analysis (PCA) and Orthogonal partial least squares-discriminant analysis (orthoPLS-DA) showed the metabolomic profile of migraine is distinguishable from controls. Volcano plot analysis identified 10 serum metabolites significantly decreased during migraine. One of these was serotonin, and the other 9 were amino acids. Pathway analysis and enrichment analysis showed tryptophan metabolism (serotonin metabolism), arginine and proline metabolism, and aminoacyl-tRNA biosynthesis are the three most prominently altered pathways in migraine. ROC curve analysis indicated Glycyl-l-proline, N-Methyl-dl-Alanine and l-Methionine are potential sensitive and specific biomarkers for migraine. Our results show Glycyl-l-proline, N-Methyl-dl-Alanine and l-Methionine may be as specific or more specific for migraine than serotonin which is the traditional biomarker of migraine. We propose that therapeutic manipulation of these metabolites or metabolic pathways may be helpful in the prevention and treatment of migraine.
[Mh] Termos MeSH primário: Alanina/análogos & derivados
Dipeptídeos/sangue
Metionina/sangue
Transtornos de Enxaqueca/diagnóstico
Serotonina/sangue
[Mh] Termos MeSH secundário: Adulto
Alanina/sangue
Arginina/sangue
Biomarcadores/sangue
Estudos de Casos e Controles
Cromatografia Líquida de Alta Pressão/métodos
Análise Discriminante
Feminino
Seres Humanos
Masculino
Metaboloma
Transtornos de Enxaqueca/sangue
Transtornos de Enxaqueca/fisiopatologia
Análise de Componente Principal
Prolina/sangue
Aminoacil-RNA de Transferência/sangue
Curva ROC
Triptofano/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Dipeptides); 0 (RNA, Transfer, Amino Acyl); 333DO1RDJY (Serotonin); 600-21-5 (N-methylalanine); 704-15-4 (glycylproline); 8DUH1N11BX (Tryptophan); 94ZLA3W45F (Arginine); 9DLQ4CIU6V (Proline); AE28F7PNPL (Methionine); OF5P57N2ZX (Alanine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


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[PMID]:29277008
[Au] Autor:Gavrina AI; Shirmanova MV; Aksenova NA; Yuzhakova DV; Snopova LB; Solovieva AB; Тimashev PS; Dudenkova VV; Zagaynova EV
[Ad] Endereço:Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation; Lobachevsky State University, 603000 Nizhny Novgorod, Russian Federation. Electronic address: niibmt@nizhgma.ru.
[Ti] Título:Photodynamic therapy of mouse tumor model using chlorin e6- polyvinyl alcohol complex.
[So] Source:J Photochem Photobiol B;178:614-622, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The use of polymeric carriers to deliver hydrophobic photosensitizers has been widely discussed as a way to improve both fluorescence diagnostic and photodynamic therapy (PDT) of cancers; however, the photophysical and pharmacokinetic parameters, as well as the PDT activity, of such modifications have, until now, only been poorly investigated. The purpose of the present study was to explore the efficacy of PDT with the formulation of the photosensitizer chlorin e6 (Ce6) in combination with polyvinyl alcohol (PVA) in comparison with Ce6 alone and with the clinical drug, Photodithazine in a mouse tumor model. We also investigated the photoactivity of the Ce6-PVA in a model reaction of tryptophan oxidation, analyzed the polymer-Ce6 interaction using fluorescence spectroscopy and atomic-force microscopy, and tested the phototoxicity in vitro. Using fluorescence imaging in vivo we found that injection to mice of Ce6 in a formulation with PVA resulted in a higher tumor-to-normal ratio and greater photobleaching when compared with either the use of Ce6 alone, or with the effects of Photodithazine. Tumor growth study and histological examination of CT26 tumors revealed fast, reproducible tumor regression and more advanced necrosis after PDT with Ce6-PVA. The higher photoactivity of the Ce6-PVA complex was confirmed in a model reaction of tryptophan oxidation and in cultured cells. Therefore, encapsulation of Ce6 in PVA represents a promising strategy for further increasing the selectivity and efficacy of PDT.
[Mh] Termos MeSH primário: Fármacos Fotossensibilizantes/química
Álcool de Polivinil/química
Porfirinas/química
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Modelos Animais de Doenças
Camundongos
Camundongos Endogâmicos BALB C
Microscopia de Força Atômica
Microscopia Confocal
Neoplasias/tratamento farmacológico
Neoplasias/patologia
Oxirredução
Fotoquimioterapia
Fármacos Fotossensibilizantes/uso terapêutico
Fármacos Fotossensibilizantes/toxicidade
Espécies Reativas de Oxigênio
Espectrometria de Fluorescência
Transplante Homólogo
Triptofano/química
Imagem Corporal Total
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 0 (Porphyrins); 0 (Reactive Oxygen Species); 5S2CCF3T1Z (phytochlorin); 8DUH1N11BX (Tryptophan); 9002-89-5 (Polyvinyl Alcohol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171226
[St] Status:MEDLINE


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[PMID]:29195411
[Au] Autor:Li R; Goswami U; Walck M; Khan K; Chen J; Cesario TC; Rentzepis PM
[Ad] Endereço:Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas 77843, USA.
[Ti] Título:Hand-held synchronous scan spectrometer for in situ and immediate detection of live/dead bacteria ratio.
[So] Source:Rev Sci Instrum;88(11):114301, 2017 Nov.
[Is] ISSN:1089-7623
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The design, construction, and operation of a hand-held synchronously scanned, excitation-emission, double monochromator spectrometer is described. Data show that it is possible to record and display within minutes the fluorescence spectra and ratio of live/dead bacteria in situ. Excitation emission matrix contour plots display clearly bacteria fluorescence spectra before and after UV inactivation, respectively. The separation of the fluorescence band maxima of molecular components, such as tryptophan, tyrosine, and DNA, may be distinguished in the diffused fluorescence spectra of bacteria and mixtures.
[Mh] Termos MeSH primário: Bactérias
Espectrometria de Fluorescência
[Mh] Termos MeSH secundário: DNA/análise
Mortalidade
Triptofano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8DUH1N11BX (Tryptophan); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE
[do] DOI:10.1063/1.4991351



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