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  1 / 18603 MEDLINE  
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[PMID]:28987406
[Au] Autor:Szewczyk R; Kusmierska A; Bernat P
[Ad] Endereço:Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lódz, Banacha 12/16, 90-237 Lódz, Poland. Electronic address: rafal.szewczyk@biol.uni.lodz.pl.
[Ti] Título:Ametryn removal by Metarhizium brunneum: Biodegradation pathway proposal and metabolic background revealed.
[So] Source:Chemosphere;190:174-183, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ametryn is a representative of a class of s-triazine herbicides absorbed by plant roots and leaves and characterized as a photosynthesis inhibitor. It is still in use in some countries in the farming of pineapples, soybean, corn, cotton, sugar cane or bananas; however, due to the adverse effects of s-triazine herbicides on living organisms use of these pesticides in the European Union has been banned. In the current study, we characterized the biodegradation of ametryn (100 mg L ) by entomopathogenic fungal cosmopolite Metarhizium brunneum. Ametryn significantly inhibited the growth and glucose uptake in fungal cultures. The concentration of the xenobiotic drops to 87.75 mg L at the end of culturing and the biodegradation process leads to formation of four metabolites: 2-hydroxy atrazine, ethyl hydroxylated ametryn, S-demethylated ametryn and deethylametryn. Inhibited growth is reflected in the metabolomics data, where significant differences in concentrations of L-proline, gamma-aminobutyric acid, L-glutamine, 4-hydroxyproline, L-glutamic acid, ornithine and L-arginine were observed in the presence of the xenobiotic when compared to control cultures. The metabolomics data demonstrated that the presence of ametryn in the fungal culture induced oxidative stress and serious disruptions of the carbon and nitrogen metabolism. Our results provide deeper insights into the microorganism strategy for xenobiotic biodegradation which may result in future enhancements to ametryn removal by the tested strain.
[Mh] Termos MeSH primário: Herbicidas/isolamento & purificação
Metarhizium/metabolismo
Triazinas/metabolismo
[Mh] Termos MeSH secundário: Atrazina
Biodegradação Ambiental
Carbono/metabolismo
Ácido Glutâmico
Herbicidas/metabolismo
Herbicidas/farmacologia
Nitrogênio/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Prolina
Saccharum/metabolismo
Triazinas/isolamento & purificação
Triazinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Triazines); 1SPQ95183Y (ametryne); 3KX376GY7L (Glutamic Acid); 7440-44-0 (Carbon); 9DLQ4CIU6V (Proline); N762921K75 (Nitrogen); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  2 / 18603 MEDLINE  
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[PMID]:29251470
[Au] Autor:Bagdi DL; Bagri GK
[Ti] Título:Effect of saline irrigation water on gas exchange and proline metabolism in ber (Ziziphus).
[So] Source:J Environ Biol;37(5):873-9, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:An experiment was conducted in pots of 25 kg capacity to study the effect of saline irrigation (EC 0,5,10,15 and 20 dSm-1) prepared by mixing NaCl, NaSO4, CaCl and MgCl2 in 3:1 ratio of chloride and sulphate on gas exchange traits, membrane stability, chlorophyll stability index and osmolytic defense mechanism in Ziziphus rotundifolia and Ziziphus nummularia species of Indian jujube (Z.mauritiana). Result showed that net photosynthetic rate (PN), transpiration (e) and stomatal conductance were comparatively lower in Ziziphus nummularia, which further declined with increasing level of saline irrigation water. Chlorophyll stability and membrane stability also declined significantly in salt stress, with higher magnitude in Ziziphus nummularia. The activity of proline anabolic enzymes; Δ1-Pyrrolline-5-carboxylate reductase, Δ1-Pyrrolline-5-carboxylate synthetase and Ornithine-δ-aminotransferase were recorded higher in Ziziphus rotundifolia with decrease in proline dehydrogenase. The sodium content was observed higher in roots of Ziziphus rotundifolia and leaves of Ziziphus nummularia. Therefore, it is suggested that salt tolerance mechanism was more efficiently operative in Ziziphus rotundifolia owing to better management of physiological attributes, osmolytic defense mechanism and restricted translocation of sodium from root to leaves along with larger accumulation of potassium in its leaves.
[Mh] Termos MeSH primário: Irrigação Agrícola
Transpiração Vegetal/efeitos dos fármacos
Salinidade
Cloreto de Sódio/química
Água/química
Ziziphus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Clorofila/metabolismo
Fotossíntese
Prolina/metabolismo
Ziziphus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 1406-65-1 (Chlorophyll); 451W47IQ8X (Sodium Chloride); 9DLQ4CIU6V (Proline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  3 / 18603 MEDLINE  
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[PMID]:29294327
[Au] Autor:Ren C; Liu J; Zhou J; Liang H; Wang Y; Sun Y; Ma B; Yin Y
[Ad] Endereço:Departments of Human Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
[Ti] Título:Low levels of serum serotonin and amino acids identified in migraine patients.
[So] Source:Biochem Biophys Res Commun;496(2):267-273, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Migraine is a highly disabling primary headache associated with a high socioeconomic burden and a generally high prevalence. The clinical management of migraine remains a challenge. This study was undertaken to identify potential serum biomarkers of migraine. Using Liquid Chromatography coupled to Mass Spectrometry (LC-MS), the metabolomic profile of migraine was compared with healthy individuals. Principal component analysis (PCA) and Orthogonal partial least squares-discriminant analysis (orthoPLS-DA) showed the metabolomic profile of migraine is distinguishable from controls. Volcano plot analysis identified 10 serum metabolites significantly decreased during migraine. One of these was serotonin, and the other 9 were amino acids. Pathway analysis and enrichment analysis showed tryptophan metabolism (serotonin metabolism), arginine and proline metabolism, and aminoacyl-tRNA biosynthesis are the three most prominently altered pathways in migraine. ROC curve analysis indicated Glycyl-l-proline, N-Methyl-dl-Alanine and l-Methionine are potential sensitive and specific biomarkers for migraine. Our results show Glycyl-l-proline, N-Methyl-dl-Alanine and l-Methionine may be as specific or more specific for migraine than serotonin which is the traditional biomarker of migraine. We propose that therapeutic manipulation of these metabolites or metabolic pathways may be helpful in the prevention and treatment of migraine.
[Mh] Termos MeSH primário: Alanina/análogos & derivados
Dipeptídeos/sangue
Metionina/sangue
Transtornos de Enxaqueca/diagnóstico
Serotonina/sangue
[Mh] Termos MeSH secundário: Adulto
Alanina/sangue
Arginina/sangue
Biomarcadores/sangue
Estudos de Casos e Controles
Cromatografia Líquida de Alta Pressão/métodos
Análise Discriminante
Feminino
Seres Humanos
Masculino
Metaboloma
Transtornos de Enxaqueca/sangue
Transtornos de Enxaqueca/fisiopatologia
Análise de Componente Principal
Prolina/sangue
Aminoacil-RNA de Transferência/sangue
Curva ROC
Triptofano/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Dipeptides); 0 (RNA, Transfer, Amino Acyl); 333DO1RDJY (Serotonin); 600-21-5 (N-methylalanine); 704-15-4 (glycylproline); 8DUH1N11BX (Tryptophan); 94ZLA3W45F (Arginine); 9DLQ4CIU6V (Proline); AE28F7PNPL (Methionine); OF5P57N2ZX (Alanine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


  4 / 18603 MEDLINE  
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[PMID]:29199976
[Au] Autor:Lee S; Wang C; Liu H; Xiong J; Jiji R; Hong X; Yan X; Chen Z; Hammel M; Wang Y; Dai S; Wang J; Jiang C; Zhang G
[Ad] Endereço:Department of Biomedical Research, National Jewish Health, Denver, CO 80206, USA.
[Ti] Título:Hydrogen bonds are a primary driving force for de novo protein folding.
[So] Source:Acta Crystallogr D Struct Biol;73(Pt 12):955-969, 2017 Dec 01.
[Is] ISSN:2059-7983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The protein-folding mechanism remains a major puzzle in life science. Purified soluble activation-induced cytidine deaminase (AID) is one of the most difficult proteins to obtain. Starting from inclusion bodies containing a C-terminally truncated version of AID (residues 1-153; AID ), an optimized in vitro folding procedure was derived to obtain large amounts of AID , which led to crystals with good quality and to final structural determination. Interestingly, it was found that the final refolding yield of the protein is proline residue-dependent. The difference in the distribution of cis and trans configurations of proline residues in the protein after complete denaturation is a major determining factor of the final yield. A point mutation of one of four proline residues to an asparagine led to a near-doubling of the yield of refolded protein after complete denaturation. It was concluded that the driving force behind protein folding could not overcome the cis-to-trans proline isomerization, or vice versa, during the protein-folding process. Furthermore, it was found that successful refolding of proteins optimally occurs at high pH values, which may mimic protein folding in vivo. It was found that high pH values could induce the polarization of peptide bonds, which may trigger the formation of protein secondary structures through hydrogen bonds. It is proposed that a hydrophobic environment coupled with negative charges is essential for protein folding. Combined with our earlier discoveries on protein-unfolding mechanisms, it is proposed that hydrogen bonds are a primary driving force for de novo protein folding.
[Mh] Termos MeSH primário: Citidina Desaminase/química
Ligações de Hidrogênio
Dobramento de Proteína
[Mh] Termos MeSH secundário: Seres Humanos
Prolina/química
Conformação Proteica
Desnaturação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9DLQ4CIU6V (Proline); EC 3.5.4.- (AICDA (activation-induced cytidine deaminase)); EC 3.5.4.5 (Cytidine Deaminase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1107/S2059798317015303


  5 / 18603 MEDLINE  
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[PMID]:29253814
[Au] Autor:Faseela P; Puthur JT
[Ad] Endereço:Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, Malappuram, Kerala 673635, India.
[Ti] Título:The imprints of the high light and UV-B stresses in Oryza sativa L. 'Kanchana' seedlings are differentially modulated.
[So] Source:J Photochem Photobiol B;178:551-559, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:High light and ultraviolet-B radiation (UV-B) are generally considered to have negative impact on photosynthesis and plant growth. The present study evaluates the tolerance potential of three cultivars of Oryza sativa L. (Kanchana, Mattatriveni and Harsha) seedlings towards high light and UV-B stress on the basis of photosynthetic pigment degradation, chlorophyll a fluorescence parameters and rate of lipid peroxidation, expressed by malondialdehyde content. Surprisingly, it was revealed that Kanchana was the most sensitive cultivar towards high light and at the same time it was the most tolerant cultivar towards UV-B stress. This contrasting feature of Kanchana towards high light and UV-B tolerance was further studied by analyzing photosystem (PS) I and II activity, mitochondrial activity, chlorophyll a fluorescence transient, enzymatic and non-enzymatic antioxidant defense system. Due to the occurrence of more PS I and PSII damages, the inhibition of photochemical efficiency and emission of dissipated energy as heat or fluorescence per PSII reaction center was higher upon high light exposure than UV-B treatments in rice seedlings of Kanchana. The mitochondrial activity was also found to be drastically altered upon high light as compared to UV-B treatments. The UV-B induced accumulation of non-enzymatic antioxidants (proline, total phenolics, sugar and ascorbate) and enzymatic antioxidants (ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase and glutathione reductase) in rice seedlings than those subjected to high light exposure afforded more efficient protection against UV-B radiation in rice seedlings. Our results proved that high tolerance of Kanchana towards UV-B than high light treatments, correlated linearly with the protected photosynthetic and mitochondrial machinery which was provided by upregulation of antioxidants particularly by total phenolics, ascorbate and ascorbate peroxidase in rice seedlings. Data presented in this study conclusively proved that rice cultivar Kanchana respond to different environmental signals independently and tolerance mechanisms to individual stress factors was also varied.
[Mh] Termos MeSH primário: Oryza/efeitos da radiação
Raios Ultravioleta
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Ascorbato Peroxidases/metabolismo
Ácido Ascórbico/metabolismo
Clorofila/química
Clorofila/metabolismo
Malondialdeído/metabolismo
Mitocôndrias/metabolismo
Mitocôndrias/efeitos da radiação
Oryza/crescimento & desenvolvimento
Fenóis/metabolismo
Fotossíntese/efeitos da radiação
Complexo de Proteína do Fotossistema I/metabolismo
Complexo de Proteína do Fotossistema II/metabolismo
Prolina/metabolismo
Plântulas/metabolismo
Plântulas/efeitos da radiação
Espectrometria de Fluorescência
Regulação para Cima/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Photosystem I Protein Complex); 0 (Photosystem II Protein Complex); 1406-65-1 (Chlorophyll); 4Y8F71G49Q (Malondialdehyde); 9DLQ4CIU6V (Proline); EC 1.11.1.11 (Ascorbate Peroxidases); PQ6CK8PD0R (Ascorbic Acid); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  6 / 18603 MEDLINE  
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[PMID]:28749490
[Au] Autor:Dmitrieva OA; Fedotova MV; Buchner R
[Ad] Endereço:G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences, Akademicheskaya st. 1, 153045 Ivanovo, Russian Federation. hebrus@mail.ru.
[Ti] Título:Evidence for cooperative Na and Cl binding by strongly hydrated l-proline.
[So] Source:Phys Chem Chem Phys;19(31):20474-20483, 2017 Aug 09.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In nature the amino acid l-proline (Pro) is a ubiquitous and highly effective osmolyte protecting cells against osmotic stress. To understand this effect knowledge of the hydration of Pro and its interactions with dissolved salts is essential. We studied these properties by combining statistical mechanics and broadband dielectric spectroscopy and found that Pro remains strongly hydrated up to high amino-acid concentrations. This is also the case upon NaCl addition to a 0.6 M Pro solution. Here, additionally a Pro·NaCl aggregate is formed with a stability constant of K° ≈ 0.95…1.25 M , where Na and Cl cooperatively bind to adjacent carboxylate-oxygen and ammonium-hydrogen atoms, respectively.
[Mh] Termos MeSH primário: Cloretos/química
Prolina/química
Sódio/química
[Mh] Termos MeSH secundário: Espectroscopia Dielétrica
Íons/química
Conformação Molecular
Pressão Osmótica
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (Ions); 059QF0KO0R (Water); 9DLQ4CIU6V (Proline); 9NEZ333N27 (Sodium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp04335j


  7 / 18603 MEDLINE  
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[PMID]:27775336
[Au] Autor:Zercher B; Hopkins TA
[Ad] Endereço:Department of Chemistry, Butler University , 4600 Sunset Avenue, Indianapolis, Indiana 46208, United States.
[Ti] Título:Induction of Circularly Polarized Luminescence from Europium by Amino Acid Based Ionic Liquids.
[So] Source:Inorg Chem;55(21):10899-10906, 2016 Nov 07.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Materials that emit circularly polarized light have application in several important industries. Because they show large optical activity and emit sharp visible light transitions, europium complexes are often exploited in applications that require circularly polarized luminescence (CPL). Chiral and coordinating ionic liquids based on prolinate, valinate, and aspartate anions are used to induce CPL from a simple achiral europium triflate salt. The sign of the induced CPL is dependent on the handedness (l vs d) of the amino acid anion. Comparison of the CPL spectra in ionic liquid with proline and valine vs aspartate shows that the number of carboxylate groups in the amino acid anion influences the europium coordination environment. DFT calculations predict a chiral eight-coordinate Eu(Pro) structure in the prolinate ionic liquid and a chiral seven- or eight-coordinate Eu(Asp) structure in the aspartate ionic liquid.
[Mh] Termos MeSH primário: Aminoácidos/química
Európio/química
Líquidos Iônicos/química
Substâncias Luminescentes/química
Mesilatos/química
[Mh] Termos MeSH secundário: Ácido Aspártico/química
Complexos de Coordenação/química
Luminescência
Medições Luminescentes
Modelos Moleculares
Prolina/química
Valina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Coordination Complexes); 0 (Ionic Liquids); 0 (Luminescent Agents); 0 (Mesylates); 30KYC7MIAI (Aspartic Acid); 444W947O8O (Europium); 9DLQ4CIU6V (Proline); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 18603 MEDLINE  
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[PMID]:28452569
[Au] Autor:Sengupta M; Raychaudhuri SS
[Ad] Endereço:a Department of Biophysics, Molecular Biology and Bioinformatics , University of Calcutta , Kolkata , India.
[Ti] Título:Partial alleviation of oxidative stress induced by gamma irradiation in Vigna radiata by polyamine treatment.
[So] Source:Int J Radiat Biol;93(8):803-817, 2017 08.
[Is] ISSN:1362-3095
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Environmental changes generate free radicals and reactive oxygen species (ROS) resulting in abiotic stress in plants. This causes alterations in germination, morphology, growth and development ultimately leading to yield loss. Gamma irradiation was used to experimentally induce oxidative damage in an important pulse crop Vigna radiata (L.) Wilczek or mung bean. Our research was aimed towards augmentation of oxidative stress tolerance through treatment with a group of aliphatic amines known as polyamines. MATERIALS AND METHODS: We used sub-lethal doses of gamma irradiation to generate oxidative damage which was evaluated using Nitro blue tetrazolium (NBT) staining, total antioxidant activity, 1, 1-Diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, proline content and lipid peroxidation. Changes in internal free polyamines and messenger ribonucleic acid (mRNA) expression of key rate-limiting S-adenosylmethionine decarboxylase (SAMDC) enzyme in polyamine biosynthetic pathway was studied using real-time polymerase chain reaction (PCR). RESULTS: We observed increased oxidative damage with higher irradiation dose which was partially alleviated by putrescine treatment. Internal levels of putrescine and spermidine increased with 1 mM (50 and 100 Gy) and 2 mM putrescine treatment. Expression of SAMDC also increased with putrescine treatment. CONCLUSION: This study shows that treatment with putrescine can partially alleviate oxidative damage caused by gamma rays.
[Mh] Termos MeSH primário: Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/efeitos da radiação
Poliaminas/farmacologia
Vigna/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Compostos de Bifenilo/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos da radiação
Peroxidação de Lipídeos/efeitos dos fármacos
Peroxidação de Lipídeos/efeitos da radiação
Picratos/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Folhas de Planta/efeitos da radiação
Proteínas de Plantas/genética
Prolina/metabolismo
Vigna/efeitos dos fármacos
Vigna/genética
Vigna/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Plant Proteins); 0 (Polyamines); 9DLQ4CIU6V (Proline); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/09553002.2017.1321807


  9 / 18603 MEDLINE  
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[PMID]:29175388
[Au] Autor:Chen Q; Zheng Y; Luo L; Yang Y; Hu X; Kong X
[Ad] Endereço:Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
[Ti] Título:Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana.
[So] Source:Biochem Biophys Res Commun;495(1):1102-1107, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flowering at the right time is important for the reproductive success of plants and their response to environmental stress. In Arabidopsis, a major determinant of natural variation in flowering time is FRIGIDA (FRI). In the present study, we show that overexpression of the functional FRIGIDA gene in wild-type Col background (ColFRI) positively enhances the drought tolerance by activating P5CS1 expression and promoting proline accumulation during water stress. Furthermore, no significant changes in FRI gene and protein expression levels were observed with drought treatment, whereas P5CS1 protein expression significantly increased. In contrast, vernalization treatment efficiently reduced P5CS1 expression levels and resulted in a decrease in drought tolerance in the ColFRI plants. The flc mutants with a functional FRI background also relieved FRI-mediated activation of P5CS1 during drought tolerance. Taken together, our findings reveal the novel function of FRI in enhancing drought resistance through its downstream P5CS1 pathway during water-deficit stress, which is dependent on its target, the FLC gene.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Arabidopsis/fisiologia
Secas
Regulação da Expressão Gênica de Plantas/fisiologia
Glutamato-5-Semialdeído Desidrogenase/metabolismo
Redes e Vias Metabólicas/fisiologia
Complexos Multienzimáticos/metabolismo
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
Estresse Fisiológico/fisiologia
[Mh] Termos MeSH secundário: Alelos
Flores/genética
Flores/crescimento & desenvolvimento
Glutamato-5-Semialdeído Desidrogenase/genética
Complexos Multienzimáticos/genética
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Plantas Geneticamente Modificadas/fisiologia
Prolina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (FRI protein, Arabidopsis); 0 (Multienzyme Complexes); 0 (delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis); 9DLQ4CIU6V (Proline); EC 1.2.1.41 (Glutamate-5-Semialdehyde Dehydrogenase); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29190818
[Au] Autor:Cortes-Hernandez P; Domínguez-Ramírez L
[Ad] Endereço:Centro de Investigacion Biomedica de Oriente (CIBIOR), Instituto Mexicano del Seguro Social (IMSS), Metepec, Puebla, Mexico.
[Ti] Título:Role of cis-trans proline isomerization in the function of pathogenic enterobacterial Periplasmic Binding Proteins.
[So] Source:PLoS One;12(11):e0188935, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Periplasmic Binding Proteins (PBPs) trap nutrients for their internalization into bacteria by ABC transporters. Ligand binding triggers PBP closure by bringing its two domains together like a Venus flytrap. The atomic determinants that control PBP opening and closure for nutrient capture and release are not known, although it is proposed that opening and ligand release occur while in contact with the ABC transporter for concurrent substrate translocation. In this paper we evaluated the effect of the isomerization of a conserved proline, located near the binding site, on the propensity of PBPs to open and close. ArgT/LAO from Salmonella typhimurium and HisJ from Escherichia coli were studied through molecular mechanics at two different temperatures: 300 and 323 K. Eight microseconds were simulated per protein to analyze protein opening and closure in the absence of the ABC transporter. We show that when the studied proline is in trans, closed empty LAO and HisJ can open. In contrast, with the proline in cis, opening transitions were much less frequent and characterized by smaller changes. The proline in trans also renders the open trap prone to close over a ligand. Our data suggest that the isomerization of this conserved proline modulates the PBP mechanism: the proline in trans allows the exploration of conformational space to produce trap opening and closure, while in cis it restricts PBP movement and could limit ligand release until in productive contact with the ABC transporter. This is the first time that a proline isomerization has been related to the control of a large conformational change like the PBP flytrap mechanism.
[Mh] Termos MeSH primário: Enterobacteriaceae/metabolismo
Proteínas Periplásmicas de Ligação/metabolismo
Prolina/metabolismo
[Mh] Termos MeSH secundário: Isomerismo
Prolina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Periplasmic Binding Proteins); 9DLQ4CIU6V (Proline)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188935



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