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[PMID]:29183668
[Au] Autor:Zheng L; Zhao XE; Ji W; Wang X; Tao Y; Sun J; Xu Y; Wang X; Zhu S; You J
[Ad] Endereço:Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong, 273165, PR China.
[Ti] Título:Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline.
[So] Source:J Chromatogr A;1532:30-39, 2018 Jan 12.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this work, a novel core-shell magnetic molecularly imprinted polymers (MMIPs) for the measurement of L-Hydroxyproline (Hyp) in dairy products was prepared. The derivative of Hyp using N-hydroxysuccinimidyl rhodamine B ester (RBS) as derivatization reagent was employed as template to prepare RBS-Hyp-MMIPs (Fe O @MIPs for RBS-Hyp). A new analytical procedure of in situ derivatization with MMIPs (ISD-MMIPs) has been developed for the specific extraction and determination of Hyp in dairy products by ultra high performance liquid chromatography tandem mass spectrometry. The RBS-Hyp-MMIPs was characterized by fourier transform infrared spectrometer and transmission electron microscopy, and evaluated by adsorption experiments. The adsorption process followed Langumuir adsorption isotherm with maximum adsorption capacity of RBS-Hyp on RBS-Hyp-MMIPs at 96 mg/g. In addition, RBS-Hyp-MMIPs showed a short equilibrium time (15.0 min), rapid magnetic separation (5 s) and high stability (retained 95.3% after six cycles). Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N > 10) at 0.1 and 0.5 ng/mL, respectively. On account of the specific extraction performance of RBS-Hyp-MMIPs, not any interference peak from real sample matrix was observed in the chromatograms of milk powder, liquid milk and milk drink. The proposed procedure was successfully applied for selective determination of Hyp from dairy products with satisfactory validation results, which is of great significance to food safety.
[Mh] Termos MeSH primário: Laticínios/análise
Análise de Alimentos/métodos
Hidroxiprolina/análise
Hidroxiprolina/química
Polímeros/química
[Mh] Termos MeSH secundário: Adsorção
Limite de Detecção
Magnetismo
Microscopia Eletrônica de Transmissão
Impressão Molecular
Reprodutibilidade dos Testes
Rodaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polymers); 0 (Rhodamines); K7G5SCF8IL (rhodamine B); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


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[PMID]:29180002
[Au] Autor:Lebda MA; Sadek KM; Abouzed TK; Tohamy HG; El-Sayed YS
[Ad] Endereço:Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Egypt. Electronic address: mohamed.a.mohamed@alexu.edu.eg.
[Ti] Título:Melatonin mitigates thioacetamide-induced hepatic fibrosis via antioxidant activity and modulation of proinflammatory cytokines and fibrogenic genes.
[So] Source:Life Sci;192:136-143, 2018 Jan 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: The potential antifibrotic effects of melatonin against induced hepatic fibrosis were explored. MAIN METHODS: Rats were allocated into four groups: placebo; thioacetamide (TAA) (200mg/kg bwt, i.p twice weekly for two months); melatonin (5mg/kgbwt, i.p daily for a week before TAA and continued for an additional two months); and melatonin plus TAA. Hepatic fibrotic changes were evaluated biochemically and histopathologically. Hepatic oxidative/antioxidative indices were assessed. The expression of hepatic proinflammatory cytokines (tumor necrosis factor-α, and interleukin-1ß), fibrogenic-related genes (transforming growth factor-1ß, collagen I, collagen, III, laminin, and autotaxin) and an antioxidant-related gene (thioredoxin-1) were detected by qRT-PCR. KEY FINDINGS: In fibrotic rats, melatonin lowered serum aspartate aminotransferase, alanine aminotransferase, and autotaxin activities, bilirubin, hepatic hydroxyproline and plasma ammonia levels. Melatonin displayed hepatoprotective and antifibrotic potential as indicated by mild hydropic degeneration of some hepatocytes and mild fibroplasia. In addition, TAA induced the depletion of glutathione, glutathione s-transferase, glutathione peroxidase, superoxide dismutase, catalase, and paraoxonase-1 (PON-1), while inducing the accumulation of malondialdehyde, protein carbonyl (C=O) and nitric oxide (NO), and DNA fragmentation. These effects were restored by melatonin pretreatment. Furthermore, melatonin markedly attenuated the expression of proinflammatory cytokines and fibrogenic genes via the upregulation of thioredoxin-1 mRNA transcripts. SIGNIFICANCE: Melatonin exhibits potent anti-inflammatory, antioxidant and fibrosuppressive activities against TAA-induced hepatic fibrogenesis via the suppression of oxidative stress, DNA damage, proinflammatory cytokines and fibrogenic gene transcripts. In addition, we demonstrate that the antifibrotic activity of melatonin is mediated by the induction of thioredoxin-1 with attenuation of autotaxin expressions.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/uso terapêutico
Antioxidantes/uso terapêutico
Citocinas/genética
Cirrose Hepática/induzido quimicamente
Cirrose Hepática/prevenção & controle
Melatonina/uso terapêutico
Tioacetamida
[Mh] Termos MeSH secundário: Animais
Hidroxiprolina/metabolismo
Fígado/efeitos dos fármacos
Fígado/metabolismo
Cirrose Hepática/genética
Testes de Função Hepática
Masculino
Estresse Oxidativo/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antioxidants); 0 (Cytokines); 0 (Protective Agents); 075T165X8M (Thioacetamide); JL5DK93RCL (Melatonin); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:29180182
[Au] Autor:Chiang N; Rodda OA; Sleigh J; Vasudevan T
[Ad] Endereço:Department of Vascular Surgery, Waikato Hospital, Hamilton, New Zealand.
[Ti] Título:Perioperative warming, oxygen, and Ilomedin on oxygenation and healing in infrainguinal bypass surgery.
[So] Source:J Surg Res;220:197-205, 2017 Dec.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Perioperative adjuncts are utilized across surgical specialities with the goal of improving patient outcomes. High-dose oxygen and extended warming are shown to increase wound collagen deposition during abdominal surgery. Prostacyclin is shown to improve limb salvage and patency rate in infrainguinal bypass (IIB) surgery. This study evaluated the impact of these adjuncts on healing and perfusion post IIB surgery. METHODS: This randomized controlled study allocated patients undergoing IIB surgery into three treatment arms (perioperative high-dose oxygen, extended warming, and a synthetic prostacyclin) or a control group. The primary outcome was accumulation of hydroxyproline (OHP, collagen surrogate marker) as collected in polytetrafluoroethylene implants on day 5. Secondary outcomes included levels of growth factors and cytokines, and tissue oxygenation of the wound and foot as measured by hyperspectral technology and ankle-brachial pressure index. Clinical outcomes were observed to day 30, with long-term follow-up of 12 mo. RESULTS: Seventy-one patients completed the study. Comparing treatment groups with the control at day 5, there were no differences in OHP, growth factors or cytokines levels, or improvement in tissue oxygenation at the surgical incision. However, there was more flow to the foot (HT-SUM (%) change) in the Ilomedin group compared to control (0% versus -14.6%, P = 0.045). HT-deoxy was higher at the peripheries in the oxygen and temperature groups, suggesting decreased tissue oxygenation. CONCLUSIONS: The perioperative treatments did not dramatically improve oxygenation or healing of the surgical wound in IIB surgery; however, Ilomedin may result in greater flow to the peripheries.
[Mh] Termos MeSH primário: Temperatura Alta/uso terapêutico
Iloprosta/uso terapêutico
Oxigênio/administração & dosagem
Assistência Perioperatória/métodos
Enxerto Vascular
Cicatrização
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Hidroxiprolina/análise
Perna (Membro)/irrigação sanguínea
Perna (Membro)/cirurgia
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
JED5K35YGL (Iloprost); RMB44WO89X (Hydroxyproline); S88TT14065 (Oxygen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


  4 / 9903 MEDLINE  
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[PMID]:28977118
[Au] Autor:Chen XJ; Liu S; Gao GZ; Yan DX; Jiang WS
[Ad] Endereço:Department of Burn and Plastic Surgery, the 253rd Hospital of People's Liberation Army, Xincheng District, Hohhot, Inner Mongolia, China.
[Ti] Título:Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits.
[So] Source:Braz J Med Biol Res;50(12):e5837, 2017 Oct 02.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p-control) and periosteum-lacking wounds treated with VSD (p-VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with continuous -120 mmHg pressure. Finally, wound tissues were harvested for analysis of hydroxyproline content and histologic detection. VSD hastened the wound healing process significantly (P<0.05) compared to the corresponding control groups. VSD alleviated the inflammation reaction, accelerated re-epithelialization and facilitated the organization of collagen fibers into neat rows. During the wound healing process, the hydroxyproline content increased overtime [i.e., postoperative days (POD) 7, POD 10 and POD 15] in all four groups, and it peaked in the p+VSD group. VSD also promoted angiogenesis via increasing number and quality of collagen. We concluded that VSD can promote healing in bone-exposed wounds via increasing hydroxyproline content and vessel density, reducing inflammatory responses and generating ordered collagen arrangement.
[Mh] Termos MeSH primário: Bandagens
Drenagem/métodos
Tratamento de Ferimentos com Pressão Negativa/métodos
Crânio/lesões
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Hidroxiprolina/análise
Microvasos
Neovascularização Fisiológica
Coelhos
Crânio/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE


  5 / 9903 MEDLINE  
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[PMID]:28877257
[Au] Autor:Bahri S; Mies F; Ben Ali R; Mlika M; Jameleddine S; Mc Entee K; Shlyonsky V
[Ad] Endereço:Department of physiology, University of Tunis El Manar, La Rabta, Tunis, Tunisia.
[Ti] Título:Rosmarinic acid potentiates carnosic acid induced apoptosis in lung fibroblasts.
[So] Source:PLoS One;12(9):e0184368, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pulmonary fibrosis is characterized by over-population and excessive activation of fibroblasts and myofibroblasts disrupting normal lung structure and functioning. Rosemary extract rich in carnosic acid (CA) and rosmarinic acid (RA) was reported to cure bleomycin-(BLM)-induced pulmonary fibrosis. We demonstrate that CA decreased human lung fibroblast (HLF) viability with IC50 value of 17.13±1.06 µM, while RA had no cytotoxic effect. In the presence of 50 µM of RA, dose-response for CA shifted to IC50 value of 11.70±1.46 µM, indicating synergic action. TGFß-transformed HLF, rat lung fibroblasts and L929 cells presented similar sensitivity to CA and CA+RA (20µM+100µM, respectively) treatment. Rat alveolar epithelial cells died only under CA+RA treatment, while A549 cells were not affected. Annexin V staining and DNA quantification suggested that HLF are arrested in G0/G1 cell cycle phase and undergo apoptosis. CA caused sustained activation of phospho-Akt and phospho-p38 expression and inhibition of p21 protein.Addition of RA potentiated these effects, while RA added alone had no action.Only triple combination of inhibitors (MAPK-p38, pan-caspase, PI3K/Akt/autophagy) partially attenuated apoptosis; this suggests that cytotoxicity of CA+RA treatment has a complex mechanism involving several parallel signaling pathways. The in vivo antifibrotic effect of CA and RA was compared with that of Vitamine-E in BLM-induced fibrosis model in rats. We found comparable reduction in fibrosis score by CA, RA and CA+RA, attenuation of collagen deposition and normalization of oxidative stress markers. In conclusion, antifibrotic effect of CA+RA is due to synergistic pro-apoptotic action on lung fibroblasts and myofibroblasts.
[Mh] Termos MeSH primário: Apoptose
Cinamatos/farmacologia
Depsídeos/farmacologia
Diterpenos Abietanos/farmacologia
Fibroblastos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bleomicina
Catalase/metabolismo
Ciclo Celular
Linhagem Celular
Movimento Celular
Proliferação Celular/efeitos dos fármacos
Colágeno/metabolismo
Seres Humanos
Hidroxiprolina/metabolismo
Concentração Inibidora 50
Peroxidação de Lipídeos
Pulmão/citologia
Masculino
Camundongos
Estresse Oxidativo
Extratos Vegetais/farmacologia
Alvéolos Pulmonares/metabolismo
Fibrose Pulmonar/tratamento farmacológico
Ratos
Ratos Wistar
Transdução de Sinais
Superóxido Dismutase/metabolismo
Vitamina E/metabolismo
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cinnamates); 0 (Depsides); 0 (Diterpenes, Abietane); 0 (Plant Extracts); 11056-06-7 (Bleomycin); 1406-18-4 (Vitamin E); 9007-34-5 (Collagen); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); LI791SXT24 (salvin); MQE6XG29YI (rosmarinic acid); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184368


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[PMID]:28662409
[Au] Autor:Li F; Han F; Li H; Zhang J; Qiao X; Shi J; Yang L; Dong J; Luo M; Wei J; Liu X
[Ad] Endereço:Center of Laboratory Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China; Department of Laboratory Medicine, College of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
[Ti] Título:Human placental mesenchymal stem cells of fetal origins-alleviated inflammation and fibrosis by attenuating MyD88 signaling in bleomycin-induced pulmonary fibrosis mice.
[So] Source:Mol Immunol;90:11-21, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pulmonary fibrosis is a progressive lung disease that its pathogenic mechanism currently is incompletely understood. Toll-like receptor (TLR) signaling has recently been identified as a regulator of inflammation and pulmonary fibrosis. In addition, mesenchymal stem cells (MSCs) of different origins offer a great promise in treatment of idiopathic pulmonary fibrosis (IPF). However mechanisms of pathogenic roles of TLR signaling and therapeutic effects of MSCs in the IPF remain elusive. In present study, the involvement of TLR signaling and the therapeutic role of MSCs were interrogated in MyD88-deficient mice using human placental MSCs of fetal origins (hfPMSCs). The results showed an alleviated pulmonary inflammation and fibrosis in myeloid differentiation primary response gene 88 (MyD88)-deficient mice treated with bleomycin (BLM), accompanied with a reduced TGF-ß signaling and production of pro-fibrotic cytokines, including TNF-α, IL-1ß. An exposure of HLF1 lung fibroblasts, A549 epithelial cells and RAW264.7 macrophages to BLM led an increased expression of key components of MyD88 and TGF-ß signaling cascades. Of interest, enforced expression and inhibition of MyD88 protein resulted in an enhanced and a reduced TGF-ß signaling in above cells in the presence of BLM, respectively. However, the addition of TGF-ß1 showed a marginally inhibitory effect on MyD88 signaling in these cells in the absence of BLM. Importantly, the administration of hfPMSCs could significantly attenuate BLM-induced pulmonary fibrosis in mice, along with a reduced hydroxyproline (HYP) deposition, MyD88 and TGF-ß signaling activation, and production of pro-fibrotic cytokines. These results may suggest an importance of MyD88/TGF-ß signaling axis in the tissue homeostasis and functional integrity of lung in response to injury, which may offer a novel target for treatment of pulmonary fibrosis.
[Mh] Termos MeSH primário: Transplante de Células-Tronco Mesenquimais
Células Mesenquimais Estromais/citologia
Fator 88 de Diferenciação Mieloide/metabolismo
Fibrose Pulmonar/patologia
Fibrose Pulmonar/terapia
[Mh] Termos MeSH secundário: Células A549
Animais
Bleomicina/toxicidade
Linhagem Celular
Feminino
Seres Humanos
Hidroxiprolina/metabolismo
Inflamação/patologia
Inflamação/terapia
Interleucina-1beta/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Fator 88 de Diferenciação Mieloide/antagonistas & inibidores
Fator 88 de Diferenciação Mieloide/genética
Placenta/citologia
Gravidez
Fibrose Pulmonar/induzido quimicamente
Transdução de Sinais
Fator de Crescimento Transformador beta/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL1B protein, mouse); 0 (Interleukin-1beta); 0 (Myd88 protein, mouse); 0 (Myeloid Differentiation Factor 88); 0 (Transforming Growth Factor beta); 0 (Tumor Necrosis Factor-alpha); 11056-06-7 (Bleomycin); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE


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[PMID]:28638889
[Au] Autor:Liu X; Jia H; Xia H
[Ad] Endereço:Orthopedics Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100007, China.
[Ti] Título:REDUCTION OF INTRA-ARTICULAR ADHESION BY TOPICAL APPLICATION OF DAIDZEIN FOLLOWING KNEE SURGERY IN RABBITS.
[So] Source:Afr J Tradit Complement Altern Med;14(4):265-271, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Intra-articular adhesion is the commonest complication that is faced by orthopedic surgeons after knee surgery. Thus, the present investigation evaluates the effect of daidzein on intra-articular adhesion in rabbits. MATERIAL AND METHODS: All the rabbits were separated in to four different groups each group carries ten rabbits. Cancellous bone was exposed in each rabbit by removing cortical bone from both side of the femoral condyle. Following daidzein (2.5, 5 and 10 mg/ml) was topically applied for the duration of 10 min to the decorticated areas. Thereafter for the period of 4 week surgical limb was fixed. Effect of daidzein on intra articular adhesion was estimated by visual score through macroscopic examination, histopathology study, hydroxyproline content, fibroblast and collage density. RESULTS: Data obtained in the study suggest that topical application of daidzein (5 and 10 mg/ml) loose the collagen and significantly decreases the adhesion at the decorticated areas. Moreover there were significant reduction in the fibroblast density, hydroxyproline content and optical density of collagen tissue in daidzein (5 and 10 mg/ml) treated group than control. CONCLUSION: Thus present study concludes that topical application of daidzein reduces intra-articular adhesion around the knee.
[Mh] Termos MeSH primário: Isoflavonas/administração & dosagem
Articulação do Joelho/cirurgia
Complicações Pós-Operatórias/tratamento farmacológico
Aderências Teciduais/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Colágeno/metabolismo
Seres Humanos
Hidroxiprolina/metabolismo
Articulação do Joelho/metabolismo
Complicações Pós-Operatórias/etiologia
Complicações Pós-Operatórias/metabolismo
Coelhos
Aderências Teciduais/etiologia
Aderências Teciduais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoflavones); 6287WC5J2L (daidzein); 9007-34-5 (Collagen); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i4.29


  8 / 9903 MEDLINE  
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[PMID]:28583732
[Au] Autor:Chiang N; Rodda OA; Sleigh J; Vasudevan T
[Ad] Endereço:Department of Vascular Surgery, Waikato Hospital, Hamilton, New Zealand.
[Ti] Título:Effects of topical negative pressure therapy on tissue oxygenation and wound healing in vascular foot wounds.
[So] Source:J Vasc Surg;66(2):564-571, 2017 Aug.
[Is] ISSN:1097-6809
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Topical negative pressure (TNP) therapy is widely used in the treatment of acute wounds in vascular patients on the basis of proposed multifactorial benefits. However, numerous recent systematic reviews have concluded that there is inadequate evidence to support its benefits at a scientific level. This study evaluated the changes in wound volume, surface area, depth, collagen deposition, and tissue oxygenation when using TNP therapy compared with traditional dressings in patients with acute high-risk foot wounds. METHODS: This study was performed with hospitalized vascular patients. Forty-eight patients were selected with an acute lower extremity wound after surgical débridement or minor amputation that had an adequate blood supply without requiring further surgical revascularization and were deemed suitable for TNP therapy. The 22 patients who completed the study were randomly allocated to a treatment group receiving TNP or to a control group receiving regular topical dressings. Wound volume and wound oxygenation were analyzed using a modern stereophotographic wound measurement system and a hyperspectral transcutaneous oxygenation measurement system, respectively. Laboratory analysis was conducted on wound biopsy samples to determine hydroxyproline levels, a surrogate marker to collagen. RESULTS: Differences in clinical or demographic characteristics or in the location of the foot wounds were not significant between the two groups. All patients, with the exception of two, had diabetes. The two patients who did not have diabetes had end-stage renal failure. There was no significance in the primary outcome of wound volume reduction between TNP and control patients on day 14 (44.2% and 20.9%, respectively; P = .15). Analyses of secondary outcomes showed a significant result of better healing rates in the TNP group by demonstrating a reduction in maximum wound depth at day 14 (36.0% TNP vs 17.6% control; P = .03). No significant findings were found for the other outcomes of changes in hydroxyproline levels (58.0% TNP vs 94.5% control; P = .32) or tissue perfusion by tissue oxyhemoglobin saturation (19.4% TNP vs 12.0% control; P = .07) at day 14. At 1 year of follow-up, there were no significant outcomes in the analysis of wound failure, major amputation, and overall survival rates between the two groups. CONCLUSIONS: In this pilot study, applying TNP to acute high-risk foot wounds in patients with diabetes or end-stage renal failure improved the wound healing rate in reference to wound depth. This suggests that TNP may play a role in enhancing wound healing. This study sets the foundation for larger studies to evaluate the superiority of TNP over traditional dressings in high-risk foot wounds.
[Mh] Termos MeSH primário: Pé Diabético/terapia
/irrigação sanguínea
Tratamento de Ferimentos com Pressão Negativa
Consumo de Oxigênio
Cicatrização
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/metabolismo
Biópsia
Monitorização Transcutânea dos Gases Sanguíneos
Colágeno/metabolismo
Pé Diabético/diagnóstico
Pé Diabético/metabolismo
Pé Diabético/patologia
Feminino
Hospitalização
Seres Humanos
Hidroxiprolina/metabolismo
Masculino
Meia-Idade
Tratamento de Ferimentos com Pressão Negativa/efeitos adversos
Nova Zelândia
Projetos Piloto
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Biomarkers); 9007-34-5 (Collagen); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE


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[PMID]:28559297
[Au] Autor:Watanabe S; Fukumori F; Miyazaki M; Tagami S; Watanabe Y
[Ad] Endereço:Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime, Japan irab@agr.ehime-u.ac.jp.
[Ti] Título:Characterization of a Novel -3-Hydroxy-l-Proline Dehydratase and a -3-Hydroxy-l-Proline Dehydratase from Bacteria.
[So] Source:J Bacteriol;199(16), 2017 Aug 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydroxyprolines, such as -4-hydroxy-l-proline (T4LHyp), -3-hydroxy-l-proline (T3LHyp), and -3-hydroxy-l-proline (C3LHyp), are present in some proteins including collagen, plant cell wall, and several peptide antibiotics. In bacteria, genes involved in the degradation of hydroxyproline are often clustered on the genome (l-Hyp gene cluster). We recently reported that an aconitase X (AcnX)-like gene from an l-Hyp gene cluster functions as a monomeric C3LHyp dehydratase (AcnX ). However, the physiological role of C3LHyp dehydratase remained unclear. We here demonstrate that NBRC 102289, an aerobic nitrogen-fixing bacterium, robustly grows using not only T4LHyp and T3LHyp but also C3LHyp as the sole carbon source. The small and large subunits of the gene ( and , respectively) from NBRC 102289 are located separately from the l-Hyp gene cluster and encode a C3LHyp dehydratase with a novel heterodimeric structure (AcnX ). A strain disrupted in the gene did not grow on C3LHyp, suggesting its involvement in C3LHyp metabolism. Furthermore, C3LHyp induced transcription of not only the genes but also the gene encoding Δ -pyrroline-2-carboxylate reductase, which is involved in T3LHyp, d-proline, and d-lysine metabolism. On the other hand, the l-Hyp gene cluster of some other bacteria contained not only the AcnX gene but also two putative proline racemase-like genes ( and ). Despite having the same active sites (a pair of Cys/Cys) as hydroxyproline 2-epimerase, which is involved in the metabolism of T4LHyp, the dominant reaction by HypA2 was clearly the dehydration of T3LHyp, a novel type of T3LHyp dehydratase that differed from the known enzyme (Cys/Thr). More than 50 years after the discovery of -4-hydroxy-l-proline (generally called l-hydroxyproline) degradation in aerobic bacteria, its genetic and molecular information has only recently been elucidated. l-Hydroxyproline metabolic genes are often clustered on bacterial genomes. These loci frequently contain a hypothetical gene(s), whose novel enzyme functions are related to the metabolism of -3-hydroxyl-proline and/or -3-hydroxyl-proline, a relatively rare l-hydroxyproline in nature. Several l-hydroxyproline metabolic enzymes show no sequential similarities, suggesting their emergence by convergent evolution. Furthermore, transcriptional regulation by -4-hydroxy-l-proline, -3-hydroxy-l-proline, and/or -3-hydroxy-l-proline significantly differs between bacteria. The results of the present study show that several l-hydroxyprolines are available for bacteria as carbon and energy sources and may contribute to the discovery of potential metabolic pathways of another hydroxyproline(s).
[Mh] Termos MeSH primário: Azospirillum brasilense/enzimologia
Hidroliases/isolamento & purificação
Hidroliases/metabolismo
Hidroxiprolina/metabolismo
[Mh] Termos MeSH secundário: Azospirillum brasilense/genética
Azospirillum brasilense/crescimento & desenvolvimento
Azospirillum brasilense/metabolismo
Carbono/metabolismo
Técnicas de Inativação de Genes
Hidroxiprolina/genética
Família Multigênica
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Subunits); 7440-44-0 (Carbon); EC 4.2.1.- (Hydro-Lyases); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE


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[PMID]:28479002
[Au] Autor:Pan J; Li D; Xu Y; Zhang J; Wang Y; Chen M; Lin S; Huang L; Chung EJ; Citrin DE; Wang Y; Hauer-Jensen M; Zhou D; Meng A
[Ad] Endereço:Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
[Ti] Título:Inhibition of Bcl-2/xl With ABT-263 Selectively Kills Senescent Type II Pneumocytes and Reverses Persistent Pulmonary Fibrosis Induced by Ionizing Radiation in Mice.
[So] Source:Int J Radiat Oncol Biol Phys;99(2):353-361, 2017 Oct 01.
[Is] ISSN:1879-355X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Ionizing radiation (IR)-induced pulmonary fibrosis (PF) is an irreversible and severe late effect of thoracic radiation therapy. The goal of this study was to determine whether clearance of senescent cells with ABT-263, a senolytic drug that can selectively kill senescent cells, can reverse PF. METHODS AND MATERIALS: C57BL/6J mice were exposed to a single dose of 17 Gy on the right side of the thorax. Sixteen weeks after IR, they were treated with 2 cycles of vehicle or ABT-263 (50 mg/kg per day for 5 days per cycle) by gavage. The effects of ABT-263 on IR-induced increases in senescent cells; elevation in the expression of selective inflammatory cytokines, matrix metalloproteinases, and tissue inhibitors of matrix metalloproteinases; and the severity of the tissue injury and fibrosis in the irradiated lungs were evaluated 3 weeks after the last treatment, in comparison with the changes observed in the irradiated lungs before treatment or after vehicle treatment. RESULTS: At 16 weeks after exposure of C57BL/6 mice to a single dose of 17 Gy, thoracic irradiation resulted in persistent PF associated with a significant increase in senescent cells. Treatment of the irradiated mice with ABT-263 after persistent PF had developed reduced senescent cells and reversed the disease. CONCLUSIONS: To our knowledge, this is the first study to demonstrate that PF can be reversed by a senolytic drug such as ABT-263 after it becomes a progressive disease. Therefore, ABT-263 has the potential to be developed as a new treatment for PF.
[Mh] Termos MeSH primário: Células Epiteliais Alveolares/efeitos dos fármacos
Compostos de Anilina/uso terapêutico
Antineoplásicos/uso terapêutico
Senescência Celular
Pneumonite por Radiação/tratamento farmacológico
Sulfonamidas/uso terapêutico
Proteína X Associada a bcl-2/antagonistas & inibidores
[Mh] Termos MeSH secundário: Células Epiteliais Alveolares/patologia
Animais
Antineoplásicos/administração & dosagem
Inibidor p16 de Quinase Dependente de Ciclina/análise
Inibidor p16 de Quinase Dependente de Ciclina/genética
Citocinas/análise
Hidroxiprolina/análise
Mediadores da Inflamação/metabolismo
Pulmão/química
Pulmão/enzimologia
Pulmão/efeitos da radiação
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Reação em Cadeia da Polimerase
Proteínas Proto-Oncogênicas c-bcl-2/análise
Proteínas Proto-Oncogênicas c-bcl-2/genética
RNA Mensageiro/análise
Pneumonite por Radiação/genética
Pneumonite por Radiação/patologia
Radiação Ionizante
Proteína X Associada a bcl-2/metabolismo
beta-Galactosidase/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Antineoplastic Agents); 0 (Cdkn2a protein, mouse); 0 (Cyclin-Dependent Kinase Inhibitor p16); 0 (Cytokines); 0 (Inflammation Mediators); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (RNA, Messenger); 0 (Sulfonamides); 0 (bcl-2-Associated X Protein); EC 3.2.1.23 (beta-Galactosidase); RMB44WO89X (Hydroxyproline); XKJ5VVK2WD (navitoclax)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE



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