Base de dados : MEDLINE
Pesquisa : D12.125.142 [Categoria DeCS]
Referências encontradas : 2009 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 201 ir para página                         

  1 / 2009 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29326050
[Au] Autor:Nayak A; Akpunarlieva S; Barrett M; Burchmore R
[Ad] Endereço:Institute of Infection, Immunity and Inflammation and Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
[Ti] Título:A defined medium for Leishmania culture allows definition of essential amino acids.
[So] Source:Exp Parasitol;185:39-52, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the sustained growth of promastigotes over multiple passages and which yields parasites that have similar infectivity to macrophages to parasites grown in a conventional semi-defined medium. We have exploited this medium to investigate the amino acid requirements of promastigotes in culture and have found that phenylalanine, tryptophan, arginine, leucine, lysine and valine are essential for viability in culture. Most of the 20 proteogenic amino acids promote growth of Leishmania promastigotes, with the exception of alanine, asparagine, and glycine. This defined medium will be useful for further studies of promastigote substrate requirements, and will facilitate future proteomic and metabolomic analyses.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/metabolismo
Meios de Cultura/química
Leishmania/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Anfotericina B/farmacologia
Animais
Antiprotozoários/farmacologia
Concentração Inibidora 50
Leishmania/efeitos dos fármacos
Leishmania donovani/crescimento & desenvolvimento
Leishmania major/crescimento & desenvolvimento
Leishmania mexicana/crescimento & desenvolvimento
Metotrexato/farmacologia
Pentamidina/farmacologia
Inoculações Seriadas
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Essential); 0 (Antiprotozoal Agents); 0 (Culture Media); 673LC5J4LQ (Pentamidine); 7XU7A7DROE (Amphotericin B); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  2 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28455617
[Au] Autor:Peng Y; Yu K; Mu C; Hang S; Che L; Zhu W
[Ad] Endereço:Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People's Republic of China.
[Ti] Título:Progressive response of large intestinal bacterial community and fermentation to the stepwise decrease of dietary crude protein level in growing pigs.
[So] Source:Appl Microbiol Biotechnol;101(13):5415-5426, 2017 Jul.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The study aimed to determine the effects of reduction of dietary crude protein (CP) level with balanced essential amino acids (EAA) on intestinal bacteria and their metabolites of growing pigs. Forty pigs (initial BW 13.50 ± 0.50 kg, 45 ± 2 days of age) were randomly assigned to four dietary treatments containing CP levels at 20.00% (normal crude protein, NP); 17.16% (medium crude protein, MP); 15.30% (low crude protein, LP); and 13.90% (extremely low crude protein, ELP), respectively. Crystalline AAs were added to meet the EAA requirement of pigs. After 4-week feeding, eight pigs per treatment (n = 8) were randomly selected and slaughtered for sampling of ileal, cecal, and colonic digesta and mucosa. Pigs with moderately reduced CP level had increased bacterial diversity, with the Shannon diversity indices for the colon digesta in the LP group and mucosa in the MP and LP groups significantly (P < 0.05) higher than those in the NP and ELP groups. As the CP level reduces, the Bifidobacterium population were linearly decreased (P < 0.05) both in ileum, cecum, and colon, and the ELP group had the lowest Bifidobacterium population in the cecum and colon, with its value significantly lower than NP and MP groups (P < 0.05). However, the ELP group had the highest population of Escherichia coli in the colon, with its value significantly higher than the LP group (P < 0.05). For bacterial metabolites, as CP level decreased, total short-chain fatty acid (T-SCFA), acetate, and butyrate were linearly increased (linear, P < 0.05) in the ileum, while all SCFAs except formate in the cecum and T-SCFA and acetate in the colon, were linearly decreased (P < 0.05). Reducing CP level led to a linear decrease of microbial crude protein (MCP) in the ileum (P < 0.05) and ammonia in all intestine segments (P < 0.05). The spermidine in cecum and total amines, cadaverine, methylamine, and spermidine in colon were shown a quadratic change (P < 0.05) as dietary CP decreases, with the highest concentration in LP group. These findings suggest that moderate reduction of dietary CP level may benefit large intestinal bacterial community and its fermentation, which was negatively affected by extremely low CP diet.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/administração & dosagem
Ração Animal
Ceco/microbiologia
Colo/microbiologia
Proteínas na Dieta/administração & dosagem
Fermentação
Consórcios Microbianos/fisiologia
[Mh] Termos MeSH secundário: Aminas/análise
Aminoácidos Essenciais/análise
Ração Animal/análise
Animais
Bifidobacterium/isolamento & purificação
Proteínas na Dieta/análise
Proteínas na Dieta/química
Digestão
Escherichia coli/isolamento & purificação
Ácidos Graxos Voláteis/metabolismo
Íleo/microbiologia
Distribuição Aleatória
Espermidina/análise
Suínos
Desmame
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Amino Acids, Essential); 0 (Dietary Proteins); 0 (Fatty Acids, Volatile); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8285-6


  3 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28573795
[Au] Autor:Abelilla JJ; Liu Y; Stein HH
[Ad] Endereço:Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
[Ti] Título:Digestible indispensable amino acid score (DIAAS) and protein digestibility corrected amino acid score (PDCAAS) in oat protein concentrate measured in 20- to 30-kilogram pigs.
[So] Source:J Sci Food Agric;98(1):410-414, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Oat protein concentrate is often used in human food, but the quality of this protein has not been characterized. Therefore, the objectives of this experiment were to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in oat protein concentrate and to determine differences in protein quality estimates between the protein digestibility-corrected AA score (PDCAAS) and the digestible indispensable AA score (DIAAS) when using growing pigs for both measurements. RESULTS: For infants, the most limiting AA in oat protein concentrate was the aromatic AA (Phe + Tyr), for which the DIAAS value was 41 and the PDCAAS was 43. For children (6 months to 3 years) and children older than 3 years, the most limiting AA in oat protein concentrate was Lys, for which the DIAAS was 56 and 67 and the PDCAAS was 58 and 69, respectively. CONCLUSION: The DIAAS value for oat protein concentrate was close to the calculated value for PDCAAS, but below the recommended intake for protein. Therefore, to satisfy the daily human AA requirement, oat protein needs to be complemented by other proteins of higher quality and specifically with greater lysine concentrations. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/metabolismo
Aminoácidos/metabolismo
Ração Animal/análise
Avena/metabolismo
Proteínas de Plantas/metabolismo
Suínos/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/análise
Aminoácidos Essenciais/análise
Animais
Avena/química
Digestão
Proteínas de Plantas/análise
Suínos/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Amino Acids, Essential); 0 (Plant Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8457


  4 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28745686
[Au] Autor:Milovanova SY; Milovanov YS; Taranova MV; Dobrosmyslov IA
[Ad] Endereço:I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia, Moscow, Russia.
[Ti] Título:[Effects of keto/amino acids and a low-protein diet on the nutritional status of patients with Stages 3B-4 chronic kidney disease].
[Ti] Título:Vliianie keto/aminokislot i ogranicheniia belka na status pitaniia bol'nykh khronicheskoi bolezn'iu pochek IIIB-IV stadii..
[So] Source:Ter Arkh;89(6):30-33, 2017.
[Is] ISSN:0040-3660
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:AIM: To evaluate the efficacy of keto/amino acids in maintaining protein balance and preventing mineral metabolic disturbances and the development of uremic hyperparathyroidism in the long-term use of a low-protein diet (LPD) in patients with Stages 3B-4 chronic kidney disease (CKD). SUBJECTS AND METHODS: Ninety patients with CKD caused by chronic latent glomerulonephritis in 65 patients and chronic tubulointerstitial nephritis of various etiologies (gout, drug-induced, and infection) in 25 were examined. The investigators conducted clinical, laboratory, and instrumental examinations, including bioelectrical impedance analysis (body mass index (BMI), the percentages of lean and fat mass), echocardiography and radiography of the abdominal aorta in the lateral projection (the presence of cardiac valvular and aortic calcification), and pulse wave velocity measurements using a Sphygmocor apparatus (vessel stiffness estimation). The stages of CKD were defined according to the 2012 Kidney Disease: Improving Global Outcomes (KDIGO) criteria; glomerular filtration rate was calculated using the CKD EPI equation. According to the diet used, all the patients were divided into 3 groups: 1) 30 patients who took LPD (0.6 g of protein per kg of body weight/day) in combination with the keto/amino acid ketosteril (1 tablet per 5 kg of body weight/day; Diet One); 2) 30 patients who used LPD in combination with the other keto/amino acid ketoaminol at the same dose (Diet Two); 3) 30 patients had LPD without using the keto/amino acids (Diet Three) (a control group). RESULTS: During a follow-up, there were no signs of malnutrition in Groups 1 and 2 patients receiving LPD (0.6 g protein per kg/day) in combination with the keto/amino acids ketosteril and ketaminol, respectively. At the same time, 11 (36.6%) patients in Group 3 (a control group) who did not take the keto/amino acids showed a BMI decrease from 24 (23; 26) kg/m2 to 18.5 (17; 19.2) kg/m2 (p < 0.05), including that of lean body mass from 37.4 (36; 38.8) to 30 (29.1; 34.7)% in the men (p<0.05) and from 29.8 (26.8; 31) to 23.9 (22; 25.7)% in the women (p<0.01). In addition, at the end of the study, there were elevated serum phosphorus levels (p<0.05) and mainly higher parathyroid hormone concentrations in Group 3 patients who received LPD without using the amino/keto acids than in Groups 1 and 2. As compared to Group 3, Groups 1 and 2 displayed no differences in the quantity of cardiac and aortic calcification and in the augmentation index (arterial stiffness). The ketosteril and ketaminol groups versus the control group had also higher s-Klotho levels (p<0.01) that were inversely correlated with glomerular filtration rate (r =-0.467; p<0.01). CONCLUSION: The keto/amino acids ketosteril or ketoaminol are an important component of LPD, which prevents malnutrition and an additional source of calcium that inhibits hyperphosphatemia and slows the development of uremic hyperparathyroidism. Incorporation of keto/amino acids into LPD leads to a less pronounced reduction in s-Klotho protein in relation to the degree of renal failure than does LPD without keto/amino acids.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/farmacologia
Aminoácidos/farmacologia
Dieta com Restrição de Proteínas/métodos
Glucuronidase/sangue
Cetoácidos/farmacologia
Avaliação de Resultados (Cuidados de Saúde)
Insuficiência Renal Crônica
[Mh] Termos MeSH secundário: Adulto
Idoso
Aminoácidos/administração & dosagem
Aminoácidos Essenciais/administração & dosagem
Terapia Combinada
Dieta com Restrição de Proteínas/efeitos adversos
Feminino
Seguimentos
Seres Humanos
Cetoácidos/administração & dosagem
Masculino
Meia-Idade
Insuficiência Renal Crônica/sangue
Insuficiência Renal Crônica/diagnóstico
Insuficiência Renal Crônica/dietoterapia
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Amino Acids, Essential); 0 (Keto Acids); 68934-50-9 (ketosteril); EC 3.2.1.31 (Glucuronidase); EC 3.2.1.31 (klotho protein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.17116/terarkh201789630-33


  5 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29053970
[Au] Autor:Wyant GA; Abu-Remaileh M; Wolfson RL; Chen WW; Freinkman E; Danai LV; Vander Heiden MG; Sabatini DM
[Ad] Endereço:Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA; Koch Institute for Integrative Cancer Research, 77 Massachuset
[Ti] Título:mTORC1 Activator SLC38A9 Is Required to Efflux Essential Amino Acids from Lysosomes and Use Protein as a Nutrient.
[So] Source:Cell;171(3):642-654.e12, 2017 Oct 19.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mTORC1 kinase is a master growth regulator that senses many environmental cues, including amino acids. Activation of mTORC1 by arginine requires SLC38A9, a poorly understood lysosomal membrane protein with homology to amino acid transporters. Here, we validate that SLC38A9 is an arginine sensor for the mTORC1 pathway, and we uncover an unexpectedly central role for SLC38A9 in amino acid homeostasis. SLC38A9 mediates the transport, in an arginine-regulated fashion, of many essential amino acids out of lysosomes, including leucine, which mTORC1 senses through the cytosolic Sestrin proteins. SLC38A9 is necessary for leucine generated via lysosomal proteolysis to exit lysosomes and activate mTORC1. Pancreatic cancer cells, which use macropinocytosed protein as a nutrient source, require SLC38A9 to form tumors. Thus, through SLC38A9, arginine serves as a lysosomal messenger that couples mTORC1 activation to the release from lysosomes of the essential amino acids needed to drive cell growth.
[Mh] Termos MeSH primário: Sistemas de Transporte de Aminoácidos/metabolismo
Aminoácidos Essenciais/metabolismo
Lisossomos/metabolismo
Complexos Multiproteicos/metabolismo
Serina-Treonina Quinases TOR/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sistemas de Transporte de Aminoácidos/química
Sistemas de Transporte de Aminoácidos/genética
Animais
Arginina/metabolismo
Linhagem Celular
Linhagem Celular Tumoral
Seres Humanos
Masculino
Alvo Mecanístico do Complexo 1 de Rapamicina
Camundongos
Camundongos Endogâmicos C57BL
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acid Transport Systems); 0 (Amino Acids, Essential); 0 (Multiprotein Complexes); 0 (SLC38A9 protein, human); 0 (SLC38A9 protein, mouse); 94ZLA3W45F (Arginine); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


  6 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28988535
[Au] Autor:Malinovsky AV
[Ad] Endereço:Biofizpribor, Branch of Federal Medical-Biological Agency, St. Petersburg, 197183, Russia. info@biofizpribor.ru.
[Ti] Título:Reason for Indispensability of Threonine in Humans and Other Mammals in Comparative Aspect.
[So] Source:Biochemistry (Mosc);82(9):1055-1060, 2017 Sep.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The essential amino acid threonine is not synthesized in vertebrates, so it must be obtained from food. During evolution, the decomposition of threonine has changed. Because the decomposition of threonine catalyzed by threonine dehydratase is irreversible, in the present work attention is focused on threonine dehydrogenase to show the inability of this enzyme to synthesize threonine in a reaction that would be the reverse of the reaction of threonine decomposition. The reason why threonine dehydrogenase cannot be used for the biosynthesis of threonine in mammalian tissues is discussed. It is concluded that some quantity of threonine is involved in transamination.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/metabolismo
Aminoácidos Essenciais
Mamíferos/metabolismo
Treonina/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Amino Acids, Essential); 2ZD004190S (Threonine); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.103 (L-threonine 3-dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917090097


  7 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28615378
[Au] Autor:van der Wielen N; Moughan PJ; Mensink M
[Ad] Endereço:Division of Human Nutrition, Wageningen University, Wageningen, Netherlands; and.
[Ti] Título:Amino Acid Absorption in the Large Intestine of Humans and Porcine Models.
[So] Source:J Nutr;147(8):1493-1498, 2017 Aug.
[Is] ISSN:1541-6100
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dietary protein quality has been recognized as a critical issue by international authorities because it can affect important functions of the body. To predict protein quality, the FAO introduced the Digestible Indispensable Amino Acid Score. This score depends on ileal amino acid (AA) digestibility; therefore, the assumption is made that AAs are not absorbed in nutritionally relevant amounts from the large intestine. This article reviews the evidence for this assumption by considering the role of the mammalian large intestine in dietary protein and AA digestion and absorption, with particular reference to adult humans. Although most dietary AAs and peptides are absorbed in the small intestine, substantial amounts can enter the large intestine. Nitrogen is absorbed in the large intestine, and a series of animal experiments indicate a potential small degree of AA absorption. In humans, colonocytes have the capacity for AA absorption because AA transporters are present in the large intestine. The absorption of nutritionally relevant amounts of dietary indispensable AAs and peptides in the human large intestine has not been convincingly demonstrated, however.
[Mh] Termos MeSH primário: Sistemas de Transporte de Aminoácidos/metabolismo
Aminoácidos Essenciais/farmacocinética
Proteínas na Dieta/farmacocinética
Digestão
Absorção Intestinal
Intestino Grosso/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos Essenciais/metabolismo
Animais
Proteínas na Dieta/metabolismo
Proteínas na Dieta/normas
Seres Humanos
Íleo/metabolismo
Nitrogênio/metabolismo
Peptídeos/metabolismo
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Amino Acid Transport Systems); 0 (Amino Acids, Essential); 0 (Dietary Proteins); 0 (Peptides); N762921K75 (Nitrogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.3945/jn.117.248187


  8 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28483523
[Au] Autor:Saito Y; Iwatsuki K; Hanyu H; Maruyama N; Aihara E; Tadaishi M; Shimizu M; Kobayashi-Hattori K
[Ad] Endereço:Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
[Ti] Título:Effect of essential amino acids on enteroids: Methionine deprivation suppresses proliferation and affects differentiation in enteroid stem cells.
[So] Source:Biochem Biophys Res Commun;488(1):171-176, 2017 Jun 17.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the effects of essential amino acids on intestinal stem cell proliferation and differentiation using murine small intestinal organoids (enteroids) from the jejunum. By selectively removing individual essential amino acids from culture medium, we found that 24 h of methionine (Met) deprivation markedly suppressed cell proliferation in enteroids. This effect was rescued when enteroids cultured in Met deprivation media for 12 h were transferred to complete medium, suggesting that Met plays an important role in enteroid cell proliferation. In addition, mRNA levels of the stem cell marker leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) decreased in enteroids grown in Met deprivation conditions. Consistent with this observation, Met deprivation also attenuated Lgr5-EGFP fluorescence intensity in enteroids. In contrast, Met deprivation enhanced mRNA levels of the enteroendocrine cell marker chromogranin A (ChgA) and markers of K cells, enterochromaffin cells, goblet cells, and Paneth cells. Immunofluorescence experiments demonstrated that Met deprivation led to an increase in the number of ChgA-positive cells. These results suggest that Met deprivation suppresses stem cell proliferation, thereby promoting differentiation. In conclusion, Met is an important nutrient in the maintenance of intestinal stem cells and Met deprivation potentially affects cell differentiation.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/farmacologia
Diferenciação Celular/efeitos dos fármacos
Metionina/farmacologia
Organoides/química
Células-Tronco/citologia
Células-Tronco/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Jejuno/química
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Essential); AE28F7PNPL (Methionine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE


  9 / 2009 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28441450
[Au] Autor:Leitão-Gonçalves R; Carvalho-Santos Z; Francisco AP; Fioreze GT; Anjos M; Baltazar C; Elias AP; Itskov PM; Piper MDW; Ribeiro C
[Ad] Endereço:Behavior and Metabolism Laboratory, Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, Portugal.
[Ti] Título:Commensal bacteria and essential amino acids control food choice behavior and reproduction.
[So] Source:PLoS Biol;15(4):e2000862, 2017 Apr.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Choosing the right nutrients to consume is essential to health and wellbeing across species. However, the factors that influence these decisions are poorly understood. This is particularly true for dietary proteins, which are important determinants of lifespan and reproduction. We show that in Drosophila melanogaster, essential amino acids (eAAs) and the concerted action of the commensal bacteria Acetobacter pomorum and Lactobacilli are critical modulators of food choice. Using a chemically defined diet, we show that the absence of any single eAA from the diet is sufficient to elicit specific appetites for amino acid (AA)-rich food. Furthermore, commensal bacteria buffer the animal from the lack of dietary eAAs: both increased yeast appetite and decreased reproduction induced by eAA deprivation are rescued by the presence of commensals. Surprisingly, these effects do not seem to be due to changes in AA titers, suggesting that gut bacteria act through a different mechanism to change behavior and reproduction. Thus, eAAs and commensal bacteria are potent modulators of feeding decisions and reproductive output. This demonstrates how the interaction of specific nutrients with the microbiome can shape behavioral decisions and life history traits.
[Mh] Termos MeSH primário: Acetobacter/fisiologia
Aminoácidos Essenciais/metabolismo
Drosophila melanogaster/microbiologia
Comportamento Alimentar
Microbioma Gastrointestinal
Lactobacillus/fisiologia
Simbiose
[Mh] Termos MeSH secundário: Acetobacter/genética
Acetobacter/crescimento & desenvolvimento
Acetobacteraceae/genética
Acetobacteraceae/crescimento & desenvolvimento
Acetobacteraceae/fisiologia
Aminoácidos Essenciais/administração & dosagem
Aminoácidos Essenciais/análise
Aminoácidos Essenciais/deficiência
Animais
Animais Geneticamente Modificados
Regulação do Apetite
Comportamento Animal
Misturas Complexas/administração & dosagem
Misturas Complexas/química
Proteínas de Drosophila/antagonistas & inibidores
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/genética
Drosophila melanogaster/fisiologia
Enterococcus faecalis/genética
Enterococcus faecalis/crescimento & desenvolvimento
Enterococcus faecalis/fisiologia
Feminino
Preferências Alimentares
Técnicas de Inativação de Genes
Interações Hospedeiro-Parasita
Lactobacillus/genética
Lactobacillus/crescimento & desenvolvimento
Oviposição
Especificidade da Espécie
Fermento Seco/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Essential); 0 (Complex Mixtures); 0 (Drosophila Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2000862


  10 / 2009 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28391610
[Au] Autor:Bonfili L; Cecarini V; Cuccioloni M; Angeletti M; Flati V; Corsetti G; Pasini E; Dioguardi FS; Eleuteri AM
[Ad] Endereço:School of Biosciences and Veterinary Medicine, University of Camerino, Italy.
[Ti] Título:Essential amino acid mixtures drive cancer cells to apoptosis through proteasome inhibition and autophagy activation.
[So] Source:FEBS J;284(11):1726-1737, 2017 Jun.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cancer cells require both energy and material to survive and duplicate in a competitive environment. Nutrients, such as amino acids (AAs), are not only a caloric source, but can also modulate cell metabolism and modify hormone homeostasis. Our hypothesis is that the environmental messages provided by AAs rule the dynamics of cancer cell life or death, and the alteration of the balance between essential amino acids (EAAs) and non-essential amino acids (NEAAs) (lower and higher than 50%, respectively) present in nutrients may represent a key instrument to alter environment-dependent messages, thus mastering cancer cells destiny. In this study, two AA mixtures, one exclusively consisting of EAAs and the other consisting of 85% EAAs and 15% NEAAs, were tested to explore their effects on the viability of both normal and cancer cell lines and to clarify the molecular mechanisms involved. Both mixtures exerted a cell-dependent anti-proliferative, cytotoxic effect involving the inhibition of proteasome activity and the consequent activation of autophagy and apoptosis. These results, besides further validating the notion of the peculiar interdependence and extensive crosstalk between the ubiquitin-proteasome system (UPS) and autophagy, indicate that variation in the ratio of EAAs and NEAAs can deeply influence cancer cell survival. Consequently, customization of dietary ratios among EAAs and NEAAs by specific AA mixtures may represent a promising anticancer strategy able to selectively induce death of cancer cells through the induction of apoptosis via both UPS inhibition and autophagy activation.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/farmacologia
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Células HCT116/efeitos dos fármacos
Células HeLa/efeitos dos fármacos
Complexo de Endopeptidases do Proteassoma/efeitos dos fármacos
Inibidores de Proteassoma/farmacologia
[Mh] Termos MeSH secundário: Clorometilcetonas de Aminoácidos/farmacologia
Mama/citologia
Células CACO-2/efeitos dos fármacos
Células CACO-2/enzimologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Células Epiteliais/efeitos dos fármacos
Feminino
Células HCT116/enzimologia
Células HeLa/enzimologia
Células Hep G2/efeitos dos fármacos
Células Hep G2/enzimologia
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acid Chloromethyl Ketones); 0 (Amino Acids, Essential); 0 (Proteasome Inhibitors); 0 (benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14081



página 1 de 201 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde