Base de dados : MEDLINE
Pesquisa : D12.644.050.200.050 [Categoria DeCS]
Referências encontradas : 972 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 98 ir para página                         

  1 / 972 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29437053
[Au] Autor:Tarabichi M; Shohat N; Goswami K; Parvizi J
[Ad] Endereço:The Rothman Institute at Thomas Jefferson University, 125 South 9th Street, Suite 1000, Philadelphia PA 19107, USA.
[Ti] Título:Can next generation sequencing play a role in detecting pathogens in synovial fluid?
[So] Source:Bone Joint J;100-B(2):127-133, 2018 02.
[Is] ISSN:2049-4408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The diagnosis of periprosthetic joint infection can be difficult due to the high rate of culture-negative infections. The aim of this study was to assess the use of next-generation sequencing for detecting organisms in synovial fluid. MATERIALS AND METHODS: In this prospective, single-blinded study, 86 anonymized samples of synovial fluid were obtained from patients undergoing aspiration of the hip or knee as part of the investigation of a periprosthetic infection. A panel of synovial fluid tests, including levels of C-reactive protein, human neutrophil elastase, total neutrophil count, alpha-defensin, and culture were performed prior to next-generation sequencing. RESULTS: Of these 86 samples, 30 were alpha-defensin-positive and culture-positive (Group I), 24 were alpha-defensin-positive and culture-negative (Group II) and 32 were alpha-defensin-negative and culture-negative (Group III). Next-generation sequencing was concordant with 25 results for Group I. In four of these, it detected antibiotic resistant bacteria whereas culture did not. In another four samples with relatively low levels of inflammatory biomarkers, culture was positive but next-generation sequencing was negative. A total of ten samples had a positive next-generation sequencing result and a negative culture. In five of these, alpha-defensin was positive and the levels of inflammatory markers were high. In the other five, alpha-defensin was negative and the levels of inflammatory markers were low. While next-generation sequencing detected several organisms in each sample, in most samples with a higher probability of infection, there was a predominant organism present, while in those presumed not to be infected, many organisms were identified with no predominant organism. CONCLUSION: Pathogens causing periprosthetic infection in both culture-positive and culture-negative samples of synovial fluid could be identified by next-generation sequencing. Cite this article: 2018;100-B:127-33.
[Mh] Termos MeSH primário: Artroplastia de Quadril
Artroplastia do Joelho
Infecções Bacterianas/microbiologia
Micoses/microbiologia
Infecções Relacionadas à Prótese/microbiologia
Análise de Sequência de DNA/métodos
Líquido Sinovial/química
Líquido Sinovial/microbiologia
[Mh] Termos MeSH secundário: Biomarcadores/análise
Proteína C-Reativa/análise
Seres Humanos
Elastase de Leucócito/análise
Reação em Cadeia da Polimerase
Estudos Prospectivos
Sensibilidade e Especificidade
Método Simples-Cego
alfa-Defensinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (alpha-Defensins); 9007-41-4 (C-Reactive Protein); EC 3.4.21.37 (Leukocyte Elastase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180214
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.100B2.BJJ-2017-0531.R2


  2 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29253854
[Au] Autor:Abdossamadi Z; Seyed N; Zahedifard F; Taheri T; Taslimi Y; Montakhab-Yeganeh H; Badirzadeh A; Vasei M; Gharibzadeh S; Rafati S
[Ad] Endereço:Department of Immunotherapy and Leishmania Vaccine Research, Pasteur institute of Iran, Tehran, Iran.
[Ti] Título:Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice.
[So] Source:PLoS Negl Trop Dis;11(12):e0006123, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human Neutrophil Peptide 1 (HNP1) produced by neutrophils, is a well-known antimicrobial peptide which plays a role both in innate as well as in adaptive immunity and is under intensive investigation as a potential therapeutic agent. Previous in vitro experiments have indicated the leishmaniacidal effect of recombinant HNP1 on Leishmania major (L. major) promastigotes and amastigotes. In the current study, we further extended the idea to explore the remedial effect of HNP1 in the two modalities of peptide therapy (folded HNP1) and gene therapy in L. major infected BALB/c mice. To this end, mice in five different groups received synthetic folded HNP1 (G1), pcDNA-HNP1-EGFP (G2), pcDNA-EGFP (G3), Amphotericin B (G4) and PBS (G5), which was started three weeks after infection for three consecutive weeks. Footpad swelling was monitored weekly and a day after the therapy ended, IFN-γ, IL-4, IL-10, IL-6 and nitric oxide produced by splenocytes were analyzed together with the parasite load in draining lymph nodes. Arginase activity and dermal histopathological changes were also analyzed in the infected footpads. We demonstrated that both therapeutic approaches effectively induced Th1 polarization and restricted parasite burden. It can control disease progression in contrast to non-treated groups. However, pcDNA-HNP1-EGFP is more promising in respect to parasite control than folded HNP1, but less effective than AmB treatment. We concluded with the call for a future approach, that is, a DNA-based expression of HNP1 combined with AmB as it can improve the leishmaniacidal efficacy.
[Mh] Termos MeSH primário: Imunoterapia/métodos
Leishmania major/efeitos dos fármacos
Leishmaniose/tratamento farmacológico
Células Th1/imunologia
Tripanossomicidas/uso terapêutico
alfa-Defensinas/uso terapêutico
[Mh] Termos MeSH secundário: Anfotericina B/uso terapêutico
Animais
Arginase/metabolismo
Células COS
Linhagem Celular
Cercopithecus aethiops
Citocinas/sangue
Feminino
Proteínas de Fluorescência Verde/genética
Leishmaniose/parasitologia
Camundongos
Camundongos Endogâmicos BALB C
Óxido Nítrico/metabolismo
Carga Parasitária
Proteínas Recombinantes/genética
Proteínas Recombinantes/uso terapêutico
alfa-Defensinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Recombinant Proteins); 0 (Trypanocidal Agents); 0 (alpha-Defensins); 0 (enhanced green fluorescent protein); 0 (human neutrophil peptide 1); 147336-22-9 (Green Fluorescent Proteins); 31C4KY9ESH (Nitric Oxide); 7XU7A7DROE (Amphotericin B); EC 3.5.3.1 (Arginase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006123


  3 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29305453
[Au] Autor:Suen K; Keeka M; Ailabouni R; Tran P
[Ad] Endereço:Western Health, 160 Gordon St, Footscray, Victoria 3011, Australia.
[Ti] Título:Synovasure 'quick test' is not as accurate as the laboratory-based α-defensin immunoassay: a systematic review and meta-analysis.
[So] Source:Bone Joint J;100-B(1):66-72, 2018 Jan.
[Is] ISSN:2049-4408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: α-defensin is a biomarker which has been described as having a high degree of accuracy in the diagnosis of periprosthetic joint infection (PJI). Current meta-analyses are based on the α-defensin laboratory-based immunoassay rather than the quick on-table lateral flow test kit. This study is the first meta-analysis to compare the accuracy of the α-defensin laboratory-based immunoassay and the lateral flow test kit for the diagnosis of PJI. MATERIALS AND METHODS: A systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Inclusion criteria were all clinical studies where the diagnosis of PJI was uncertain. All studies selected used the Musculoskeletal Infection Society (MSIS) or modified MSIS criteria. Two independent reviewers reviewed the studies and extracted data. A meta-analysis of results was carried out: pooled sensitivity, specificity, positive and negative likelihood ratio, heterogeneity and areas under curves are reported. RESULTS: Ten studies (759 patients) were included. Of these, seven studies (640 patients) evaluated the laboratory-based α-defensin immunoassay and three (119 patients) the lateral flow test. The pooled sensitivity and specificity of the qualitative α-defensin laboratory immunoassay was 0.953 (95% confidence interval (CI) 0.87 to 0.984) and 0.965 (95% CI 0.943 to 0.979) respectively. The pooled positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were 34.86 (95% CI 19.34 to 62.85) and 0.02 (95% CI 0.00 to 0.11). The pooled sensitivity and specificity of the lateral flow test were 0.774 (95% CI 0.637 to 0.870) and 0.913 (95% CI 0.828 to 0.958), respectively. The pooled PLR and NLR were 8.675 (95% CI 4.229 to 17.794) and 0.248 (95% CI 0.147 to 0.418), respectively. CONCLUSION: The pooled sensitivity and specificity of the lateral flow test were lower than those of the α-defensin laboratory-based immunoassay test. Hence, care must be taken with interpretation of the lateral flow test when relying on its results for the intra-operative diagnosis of PJI. Cite this article: 2018;100-B:66-72.
[Mh] Termos MeSH primário: Prótese Articular/efeitos adversos
Infecções Relacionadas à Prótese/diagnóstico
Kit de Reagentes para Diagnóstico
Líquido Sinovial/química
alfa-Defensinas/análise
[Mh] Termos MeSH secundário: Biomarcadores/análise
Seres Humanos
Imunoensaio
Falha de Prótese/etiologia
Reoperação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Reagent Kits, Diagnostic); 0 (alpha-Defensins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.100B1.BJJ-2017-0630.R1


  4 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28449029
[Au] Autor:Munz M; Willenborg C; Richter GM; Jockel-Schneider Y; Graetz C; Staufenbiel I; Wellmann J; Berger K; Krone B; Hoffmann P; van der Velde N; Uitterlinden AG; de Groot LCPGM; Sawalha AH; Direskeneli H; Saruhan-Direskeneli G; Guzeldemir-Akcakanat E; Keceli G; Laudes M; Noack B; Teumer A; Holtfreter B; Kocher T; Eickholz P; Meyle J; Doerfer C; Bruckmann C; Lieb W; Franke A; Schreiber S; Nohutcu RM; Erdmann J; Loos BG; Jepsen S; Dommisch H; Schaefer AS
[Ad] Endereço:Department of Periodontology and Synoptic Dentistry, Institute of Dental, Oral and Maxillary Medicine, Charité - University Medicine Berlin, Germany.
[Ti] Título:A genome-wide association study identifies nucleotide variants at SIGLEC5 and DEFA1A3 as risk loci for periodontitis.
[So] Source:Hum Mol Genet;26(13):2577-2588, 2017 07 01.
[Is] ISSN:1460-2083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Periodontitis is one of the most common inflammatory diseases, with a prevalence of 11% worldwide for the severe forms and an estimated heritability of 50%. The disease is characterized by destruction of the alveolar bone due to an aberrant host inflammatory response to a dysbiotic oral microbiome. Previous genome-wide association studies (GWAS) have reported several suggestive susceptibility loci. Here, we conducted a GWAS using a German and Dutch case-control sample of aggressive periodontitis (AgP, 896 cases, 7,104 controls), a rare but highly severe and early-onset form of periodontitis, validated the associations in a German sample of severe forms of the more moderate phenotype chronic periodontitis (CP) (993 cases, 1,419 controls). Positive findings were replicated in a Turkish sample of AgP (223 cases, 564 controls). A locus at SIGLEC5 (sialic acid binding Ig-like lectin 5) and a chromosomal region downstream of the DEFA1A3 locus (defensin alpha 1-3) showed association with both disease phenotypes and were associated with periodontitis at a genome-wide significance level in the pooled samples, with P = 1.09E-08 (rs4284742,-G; OR = 1.34, 95% CI = 1.21-1.48) and P = 5.48E-10 (rs2738058,-T; OR = 1.28, 95% CI = 1.18-1.38), respectively. SIGLEC5 is expressed in various myeloid immune cells and classified as an inhibitory receptor with the potential to mediate tyrosine phosphatases SHP-1/-2 dependent signaling. Alpha defensins are antimicrobial peptides with expression in neutrophils and mucosal surfaces and a role in phagocyte-mediated host defense. This study identifies the first shared genetic risk loci of AgP and CP with genome-wide significance and highlights the role of innate and adaptive immunity in the etiology of periodontitis.
[Mh] Termos MeSH primário: Antígenos CD/genética
Antígenos de Diferenciação Mielomonocítica/genética
Periodontite Crônica/genética
Lectinas/genética
Peptídeos Cíclicos/genética
alfa-Defensinas/genética
[Mh] Termos MeSH secundário: Adulto
Periodontite Agressiva/genética
Antígenos CD/metabolismo
Antígenos de Diferenciação Mielomonocítica/metabolismo
Estudos de Casos e Controles
Feminino
Loci Gênicos
Predisposição Genética para Doença
Estudo de Associação Genômica Ampla
Genótipo
Seres Humanos
Lectinas/metabolismo
Masculino
Meia-Idade
Nucleotídeos
Peptídeos Cíclicos/metabolismo
Fenótipo
Polimorfismo de Nucleotídeo Único/genética
Fatores de Risco
Turquia
alfa-Defensinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (DEFA1A3 protein, human); 0 (Lectins); 0 (Nucleotides); 0 (Peptides, Cyclic); 0 (SIGLEC5 protein, human); 0 (alpha-Defensins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/hmg/ddx151


  5 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29257013
[Au] Autor:Lee YS; Koo KH; Kim HJ; Tian S; Kim TY; Maltenfort MG; Chen AF
[Ad] Endereço:Department of Orthopedic Surgery, Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.
[Ti] Título:Synovial Fluid Biomarkers for the Diagnosis of Periprosthetic Joint Infection: A Systematic Review and Meta-Analysis.
[So] Source:J Bone Joint Surg Am;99(24):2077-2084, 2017 Dec 20.
[Is] ISSN:1535-1386
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The search for a single standard reference test for determining periprosthetic joint infection (PJI) through analysis of synovial fluid has yielded numerous biomarkers as potential candidates. The purpose of the present systematic review and meta-analysis was to evaluate the diagnostic accuracy of synovial fluid biomarkers and to determine which test has the highest diagnostic odds ratio (DOR) for the diagnosis of PJI. METHODS: An online literature search of the MEDLINE, Embase, and Cochrane databases identified 33 articles reporting a total of 13 major parameters for diagnosing PJI through analysis of synovial fluid. Each of the included articles was independently analyzed for risk of bias and for concerns regarding applicability utilizing the QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies-2) tool. The mada (meta-analysis of diagnostic accuracy) tool was used to generate forest plots for sensitivity, specificity, and the log of the DOR, as well as summary statistics. RESULTS: In this analysis, 13 index tests (leukocyte count; measurement of the percentage of polymorphonucleocytes [PMN%] and the levels of C-reactive protein [CRP], α-defensin, leukocyte esterase [LE], interleukin [IL]-6, IL-8, IL-10, IL-1ß, vascular endothelial growth factor [VEGF], and granulocyte-colony stimulating factor [G-CSF]; culture; and polymerase chain reaction [PCR] analysis) were evaluated on the basis of ≥2 articles. Of these tests, 8 (leukocyte count, PMN%, CRP, α-defensin, LE, IL-6, IL-8, and culture) were appropriate for pooled analysis. The overall sensitivity of these 8 markers was 0.85, and all but culture showed a sensitivity of ≥0.8. All markers showed a specificity of ≥0.9. Of the 8 tests, measurement of the α-defensin level showed the highest log DOR. CONCLUSIONS: Synovial fluid leukocyte count, PMN%, CRP, α-defensin, LE, IL-6, and IL-8 all demonstrated high sensitivity for diagnosing PJI, with α-defensin being the best synovial marker based on the highest log DOR. However, other synovial fluid tests that demonstrate good diagnostic performance can also be used in combination for the diagnosis of PJI. LEVEL OF EVIDENCE: Diagnostic Level IV. See Instructions for Authors for a complete description of levels of evidence.
[Mh] Termos MeSH primário: Artroplastia de Substituição/efeitos adversos
Infecções Relacionadas à Prótese/diagnóstico
Líquido Sinovial/metabolismo
alfa-Defensinas/metabolismo
[Mh] Termos MeSH secundário: Artroplastia de Substituição/métodos
Biomarcadores/análise
Proteína C-Reativa/metabolismo
Feminino
Seres Humanos
Masculino
Sensibilidade e Especificidade
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Vascular Endothelial Growth Factor A); 0 (alpha-Defensins); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.2106/JBJS.17.00123


  6 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28466260
[Au] Autor:Singh SB; Wilson M; Ritz N; Lin HC
[Ad] Endereço:Section of Gastroenterology, Medicine Service, New Mexico VA Health Care System, 1501 San Pedro SE, Albuquerque, NM, 87108, USA.
[Ti] Título:Autophagy Genes of Host Responds to Disruption of Gut Microbial Community by Antibiotics.
[So] Source:Dig Dis Sci;62(6):1486-1497, 2017 06.
[Is] ISSN:1573-2568
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Defective autophagic machinery, such as that in Crohn's disease patients homozygous for ATG16L1 risk allele, is associated with alteration of resident gut bacterial communities. However, whether or not host autophagy responds to changes in the resident gut microbial community is not known. Here, we investigated the effect of antibiotic-induced disruption of the gut microbiome (dysbiosis) on autophagy gene expression and the expression of antimicrobial peptides/protein (AMP) over time. AIM: To test the hypothesis that antibiotic treatment may cause time-dependent changes in gut bacterial density, autophagy genes, and antimicrobial protein/peptide gene expression. METHODS: Mice (n = 8 per group) were treated with antibiotic cocktail and sacrificed at different intervals of recovery (days 3, 7, 10, 14, 21, 28, 35, and 42) post-antibiotics. DNA and RNA were extracted from small intestinal tissues. Bacterial density, expression of host autophagy genes, and AMP genes were analyzed by relative quantitative PCR. Fold change difference in comparison with untreated control group was calculated using 2 method. Statistical analysis was performed using nonparametric Mann-Whitney test. RESULTS: Gut bacterial density changed in a time-dependent fashion in response to antibiotic treatment. These changes were concurrent with upregulation of autophagy genes and antimicrobial peptide/protein gene expression. We further showed that an oral gavage of a resident microbe Desulfovibrio, which bloomed in antibiotic-treated animals, induced Atg5 and lysozyme (Lyz) gene expression. CONCLUSION: Autophagy genes respond to dysbiosis induced by antibiotics. This response may be a host mechanism to detect and possibly correct dysbiosis by activating antimicrobial peptides/proteins that control the microbial load in the gut.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Peptídeos Catiônicos Antimicrobianos/genética
Autofagia/genética
Disbiose/microbiologia
Microbioma Gastrointestinal/efeitos dos fármacos
RNA Ribossômico 16S/análise
[Mh] Termos MeSH secundário: Animais
Proteína 5 Relacionada à Autofagia/genética
Proteínas Relacionadas à Autofagia/genética
Bacteroidetes
Células Cultivadas
Desulfovibrio
Desulfovibrio vulgaris
Disbiose/induzido quimicamente
Disbiose/genética
Células Epiteliais/efeitos dos fármacos
Feminino
Firmicutes
Expressão Gênica
Intestino Delgado/citologia
Intestino Delgado/microbiologia
Macrófagos/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos C57BL
Muramidase/genética
Proteínas Associadas a Pancreatite
Proteínas/genética
Fatores de Tempo
Regulação para Cima
alfa-Defensinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antimicrobial Cationic Peptides); 0 (Atg5 protein, mouse); 0 (Autophagy-Related Protein 5); 0 (Autophagy-Related Proteins); 0 (Pancreatitis-Associated Proteins); 0 (Proteins); 0 (RNA, Ribosomal, 16S); 0 (Reg3g protein, mouse); 0 (alpha-Defensins); 0 (cryptdin 4, mouse); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s10620-017-4589-8


  7 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28910369
[Au] Autor:Kumar NP; Moideen K; Viswanathan V; Sivakumar S; Menon PA; Kornfeld H; Babu S
[Ad] Endereço:National Institutes of Health-NIRT-International Center for Excellence in Research, Chennai, India.
[Ti] Título:Heightened circulating levels of antimicrobial peptides in tuberculosis-Diabetes co-morbidity and reversal upon treatment.
[So] Source:PLoS One;12(9):e0184753, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The association of antimicrobial peptides (AMPs) with tuberculosis-diabetes comorbidity (PTB-DM) is not well understood. METHODS: To study the association of AMPs with PTB-DM, we examined the systemic levels of cathelicidin (LL37), human beta defensin- 2 (HBD2), human neutrophil peptides 1-3, (HNP1-3) and granulysin in individuals with either PTB-DM, PTB, latent TB (LTB) or no TB infection (NTB). RESULTS: Circulating levels of cathelicidin and HBD2 were significantly higher and granulysin levels were significantly lower in PTB-DM compared to PTB, LTB or NTB, while the levels of HNP1-3 were significantly higher in PTB-DM compared to LTB or NTB individuals. Moreover, the levels of cathelicidin and/or HBD2 were significantly higher in PTB-DM or PTB individuals with bilateral and cavitary disease and also exhibited a significant positive relationship with bacterial burden. Cathelidin, HBD2 and HNP1-3 levels exhibited a positive relationship with HbA1c and/or fasting blood glucose levels. Finally, anti-tuberculosis therapy resulted in significantly diminished levels of cathelicidin, HBD2, granulysin and significantly enhanced levels of HNP1-3 and granulysin in PTB-DM and/or PTB individuals. CONCLUSION: Therefore, our data demonstrate that PTB-DM is associated with markedly enhanced levels of AMPs and diminished levels of granulysin.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/sangue
Antituberculosos/uso terapêutico
Diabetes Mellitus Tipo 2/metabolismo
Tuberculose Pulmonar/tratamento farmacológico
alfa-Defensinas/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Antígenos de Diferenciação de Linfócitos T/sangue
Catelicidinas/sangue
Comorbidade
Feminino
Seres Humanos
Masculino
Meia-Idade
Tuberculose Pulmonar/metabolismo
Adulto Jovem
beta-Defensinas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Differentiation, T-Lymphocyte); 0 (Antimicrobial Cationic Peptides); 0 (Antitubercular Agents); 0 (Cathelicidins); 0 (DEFB4A protein, human); 0 (GNLY protein, human); 0 (alpha-Defensins); 0 (beta-Defensins); 0 (cathelicidin antimicrobial peptide); 0 (human neutrophil peptide 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184753


  8 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28860397
[Au] Autor:Berger P; Van Cauter M; Driesen R; Neyt J; Cornu O; Bellemans J
[Ad] Endereço:University Hospitals Leuven, Weligerveld 1, 3212 Pellenberg, Belgium.
[Ti] Título:Diagnosis of prosthetic joint infection with alpha-defensin using a lateral flow device: a multicentre study.
[So] Source:Bone Joint J;99-B(9):1176-1182, 2017 Sep.
[Is] ISSN:2049-4408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The purpose of this current multicentre study is to analyse the presence of alpha-defensin proteins in synovial fluid using the Synovasure lateral flow device and to determine its diagnostic reliability and accuracy compared with the prosthetic joint infection (PJI) criteria produced by the Musculoskeletal Infection Society (MSIS). PATIENTS AND METHODS: A cohort of 121 patients comprising 85 total knee arthroplasties and 36 total hip arthroplasties was prospectively evaluated between May 2015 and June 2016 in three different orthopaedic centres. The tests were performed on patients with a chronically painful prosthesis undergoing a joint aspiration in a diagnostic pathway or during revision surgery. RESULTS: Based on the MSIS criteria, 34 patients (28%) would have had a PJI, and 87 patients had no PJI. Testing with the lateral flow device had a sensitivity of 97.1% (95% confidence intervals (CI) 84.5 to 99.9) and a specificity of 96.6% (95% CI 90.3 to 99.2). The positive predictive value was 91.7% (95% CI 77.7% to 98.3), and the negative predictive value was 98.8% (95% CI 93.6 to 99.9). Receiver operator characteristics analysis demonstrated an area under the curve for the Synovasure test of 0.97 (95% CI 0.93 to 1.00). CONCLUSION: Our findings suggest that the Synovasure test has an excellent diagnostic performance to confirm or reject the diagnosis of a PJI. The results are promising for the care of the painful or problematic knee and hip joint arthroplasty and the test should be considered as part of the diagnostic toolbox for PJIs. Cite this article: 2017;99-B:1176-82.
[Mh] Termos MeSH primário: Artroplastia de Quadril
Artroplastia do Joelho
Infecções Relacionadas à Prótese/diagnóstico
Infecções Relacionadas à Prótese/metabolismo
alfa-Defensinas/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/metabolismo
Feminino
Seres Humanos
Masculino
Meia-Idade
Valor Preditivo dos Testes
Estudos Prospectivos
Infecções Relacionadas à Prótese/terapia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Biomarkers); 0 (alpha-Defensins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.99B9.BJJ-2016-1345.R2


  9 / 972 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28817680
[Au] Autor:Williams AD; Korolkova OY; Sakwe AM; Geiger TM; James SD; Muldoon RL; Herline AJ; Goodwin JS; Izban MG; Washington MK; Smoot DT; Ballard BR; Gazouli M; M'Koma AE
[Ad] Endereço:Department of Microbiology and Immunology, Meharry Medical College School of Medicine, Nashville, Tennessee, United States of America.
[Ti] Título:Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease.
[So] Source:PLoS One;12(8):e0179710, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inability to distinguish Crohn's colitis from ulcerative colitis leads to the diagnosis of indeterminate colitis. This greatly effects medical and surgical care of the patient because treatments for the two diseases vary. Approximately 30 percent of inflammatory bowel disease patients cannot be accurately diagnosed, increasing their risk of inappropriate treatment. We sought to determine whether transcriptomic patterns could be used to develop diagnostic biomarker(s) to delineate inflammatory bowel disease more accurately. Four patients groups were assessed via whole-transcriptome microarray, qPCR, Western blot, and immunohistochemistry for differential expression of Human α-Defensin-5. In addition, immunohistochemistry for Paneth cells and Lysozyme, a Paneth cell marker, was also performed. Aberrant expression of Human α-Defensin-5 levels using transcript, Western blot, and immunohistochemistry staining levels was significantly upregulated in Crohn's colitis, p< 0.0001. Among patients with indeterminate colitis, Human α-Defensin-5 is a reliable differentiator with a positive predictive value of 96 percent. We also observed abundant ectopic crypt Paneth cells in all colectomy tissue samples of Crohn's colitis patients. In a retrospective study, we show that Human α-Defensin-5 could be used in indeterminate colitis patients to determine if they have either ulcerative colitis (low levels of Human α-Defensin-5) or Crohn's colitis (high levels of Human α-Defensin-5). Twenty of 67 patients (30 percent) who underwent restorative proctocolectomy for definitive ulcerative colitis were clinically changed to de novo Crohn's disease. These patients were profiled by Human α-Defensin-5 immunohistochemistry. All patients tested strongly positive. In addition, we observed by both hematoxylin and eosin and Lysozyme staining, a large number of ectopic Paneth cells in the colonic crypt of Crohn's colitis patient samples. Our experiments are the first to show that Human α-Defensin-5 is a potential candidate biomarker to molecularly differentiate Crohn's colitis from ulcerative colitis, to our knowledge. These data give us both a potential diagnostic marker in Human α-Defensin-5 and insight to develop future mechanistic studies to better understand crypt biology in Crohn's colitis.
[Mh] Termos MeSH primário: Biomarcadores
Doenças Inflamatórias Intestinais/metabolismo
alfa-Defensinas/metabolismo
[Mh] Termos MeSH secundário: Biópsia
Colite Ulcerativa/diagnóstico
Colite Ulcerativa/metabolismo
Doença de Crohn/diagnóstico
Doença de Crohn/metabolismo
Diagnóstico Diferencial
Perfilação da Expressão Gênica
Seres Humanos
Imuno-Histoquímica
Doenças Inflamatórias Intestinais/diagnóstico
Doenças Inflamatórias Intestinais/genética
Doenças Inflamatórias Intestinais/cirurgia
Mucosa Intestinal/metabolismo
Mucosa Intestinal/patologia
Muramidase/metabolismo
Proctocolectomia Restauradora
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (alpha-Defensins); 0 (alpha-defensin 5, human); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179710


  10 / 972 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28715972
[Au] Autor:Holly MK; Diaz K; Smith JG
[Ad] Endereço:Department of Microbiology, University of Washington, Seattle, Washington 98195; email: jgsmith2@uw.edu.
[Ti] Título:Defensins in Viral Infection and Pathogenesis.
[So] Source:Annu Rev Virol;4(1):369-391, 2017 Sep 29.
[Is] ISSN:2327-0578
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:α, ß, and θ defensins are effectors of the innate immune system with potent antibacterial, antiviral, and antifungal activity. Defensins have direct antiviral activity in cell culture, with varied mechanisms for individual viruses, although some common themes have emerged. In addition, defensins have potent immunomodulatory activity that can alter innate and adaptive immune responses to viral infection. In some cases, there is evidence for paradoxical escape from defensin neutralization or enhancement of viral infection. The direct and indirect activities of defensins have led to their development as therapeutics and vaccine components. The major area of investigation that continues to lag is the connection between the effects of defensins in cell culture models and viral pathogenesis in vivo. Model systems to study defensin biology, including more physiologic models designed to bridge this gap, are also discussed.
[Mh] Termos MeSH primário: Defensinas/metabolismo
Viroses/imunologia
alfa-Defensinas/metabolismo
beta-Defensinas/metabolismo
[Mh] Termos MeSH secundário: Adenoviridae/efeitos dos fármacos
Adenoviridae/patogenicidade
Animais
Antivirais/farmacologia
Defensinas/genética
Defensinas/farmacologia
Defensinas/uso terapêutico
HIV/efeitos dos fármacos
HIV/patogenicidade
Herpesviridae/efeitos dos fármacos
Herpesviridae/patogenicidade
Seres Humanos
Imunidade Inata
Imunomodulação
Camundongos
Papillomaviridae/efeitos dos fármacos
Papillomaviridae/patogenicidade
Viroses/tratamento farmacológico
alfa-Defensinas/genética
alfa-Defensinas/farmacologia
alfa-Defensinas/uso terapêutico
beta-Defensinas/genética
beta-Defensinas/farmacologia
beta-Defensinas/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Defensins); 0 (alpha-Defensins); 0 (beta-Defensins); 0 (theta-defensin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-virology-101416-041734



página 1 de 98 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde