Base de dados : MEDLINE
Pesquisa : D12.644.233.800 [Categoria DeCS]
Referências encontradas : 8204 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 821 ir para página                         

  1 / 8204 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28747404
[Au] Autor:Lim H; Yu CY; Jou TS
[Ad] Endereço:Graduate Institute of Molecular Medicine National Taiwan University, Taipei, Taiwan.
[Ti] Título:Galectin-8 regulates targeting of Gp135/podocalyxin and lumen formation at the apical surface of renal epithelial cells.
[So] Source:FASEB J;31(11):4917-4927, 2017 11.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Establishment of apical-basal polarity, through correct targeting of polarity determinants to distinct domains of the plasma membrane, is a fundamental process for the development of functioning epithelial tubules. Here we report that galectin (Gal)-8 regulates apical-basal polarity of Madin-Darby canine kidney (MDCK) cells apical targeting of 135-kDa glycoprotein (Gp135). Gal-8 interacts with newly synthesized Gp135 in a glycan-dependent manner. Gal-8 knockdown induces aberrant lumens at the lateral domain and mistargeting of Gp135 to this structure, thus disrupting the kidney epithelial polarity of MDCK cells, which organize lumens at the apical surface. The -glycosylation deletion mutant of Gp135 phenocopies the effect of Gal-8 knockdown, which suggests that Gal-8 is the decoding machinery for the apical sorting signals of Gp135 residing at its -glycosylation-rich region. Collectively, our results reveal a new role of Gal-8 in the development of luminal organs by regulating targeting of apical polarity protein Gp135.-Lim, H., Yu, C.-Y., Jou, T.-S. Galectin-8 regulates targeting of Gp135/podocalyxin and lumen formation at the apical surface of renal epithelial cells.
[Mh] Termos MeSH primário: Polaridade Celular/fisiologia
Células Epiteliais/metabolismo
Galectinas/metabolismo
Rim/metabolismo
Sialoglicoproteínas/metabolismo
[Mh] Termos MeSH secundário: Animais
Cães
Células Epiteliais/citologia
Galectinas/genética
Rim/citologia
Células Madin Darby de Rim Canino
Sialoglicoproteínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Galectins); 0 (Sialoglycoproteins); 0 (podocalyxin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201601386R


  2 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29259037
[Au] Autor:Falch CM; Sundaram AYM; Øystese KA; Normann KR; Lekva T; Silamikelis I; Eieland AK; Andersen M; Bollerslev J; Olarescu NC
[Ad] Endereço:Section of Specialized EndocrinologyDepartment of Endocrinology cafal14@student.sdu.dk.
[Ti] Título:Gene expression profiling of fast- and slow-growing non-functioning gonadotroph pituitary adenomas.
[So] Source:Eur J Endocrinol;178(3):295-307, 2018 Mar.
[Is] ISSN:1479-683X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Reliable biomarkers associated with aggressiveness of non-functioning gonadotroph adenomas (GAs) are lacking. As the growth of tumor remnants is highly variable, molecular markers for growth potential prediction are necessary. We hypothesized that fast- and slow-growing GAs present different gene expression profiles and reliable biomarkers for tumor growth potential could be identified, focusing on the specific role of epithelial-mesenchymal transition (EMT). DESIGN AND METHODS: Eight GAs selected for RNA sequencing were equally divided into fast- and slow-growing group by the tumor volume doubling time (TVDT) median (27.75 months). Data were analyzed by tophat2, cufflinks and cummeRbund pipeline. 40 genes were selected for RT-qPCR validation in 20 GAs based on significance, fold-change and pathway analyses. The effect of silencing (metadherin) and (endomucin) on migration of human adenoma cells was evaluated. RESULTS: 350 genes were significantly differentially expressed (282 genes upregulated and 68 downregulated in the fast group, -adjusted <0.05). Among 40 selected genes, 11 showed associations with TVDT (-0.669< <-0.46, < 0.05). These were and six EMT-related genes ( and ). , but not , demonstrated involvement in cell migration and association with EMT markers. CONCLUSIONS: Fast- and slow-growing GAs present different gene expression profiles, and genes related to EMT have higher expression in fast-growing tumors. In addition to , identified as an important contributor to aggressiveness, the other genes might represent markers for tumor growth potential and possible targets for drug therapy.
[Mh] Termos MeSH primário: Adenoma/genética
Transição Epitelial-Mesenquimal/genética
Neoplasias Hipofisárias/genética
RNA Mensageiro/metabolismo
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/genética
Adenoma/metabolismo
Adulto
Idoso
Caderinas/genética
Moléculas de Adesão Celular/genética
Movimento Celular/genética
Subunidades Catalíticas da Proteína Quinase Dependente de AMP Cíclico/genética
Feminino
Hormônio Foliculoestimulante/metabolismo
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Inativação Gênica
Seres Humanos
Técnicas In Vitro
Peptídeos e Proteínas de Sinalização Intracelular/genética
Hormônio Luteinizante/metabolismo
Masculino
Glicoproteínas de Membrana/genética
Proteínas Associadas aos Microtúbulos/genética
Meia-Idade
Miosina Tipo I/genética
Proteínas do Tecido Nervoso/genética
Neoplasias Hipofisárias/metabolismo
Proteínas Proto-Oncogênicas/genética
Receptores de Superfície Celular/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ribonucleases/genética
Sialoglicoproteínas/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Cadherins); 0 (Cell Adhesion Molecules); 0 (EMCN protein, human); 0 (GPM6A protein, human); 0 (Intracellular Signaling Peptides and Proteins); 0 (MTDH protein, human); 0 (MYO1B protein, human); 0 (Membrane Glycoproteins); 0 (Microtubule-Associated Proteins); 0 (Nerve Tissue Proteins); 0 (PCDH18 protein, human); 0 (Proto-Oncogene Proteins); 0 (RNA, Messenger); 0 (Receptors, Cell Surface); 0 (SKIL protein, human); 0 (SPAG9 protein, human); 0 (Sialoglycoproteins); 0 (UNC5H4 protein, human); 0 (hook1 protein, human); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Catalytic Subunits); EC 2.7.11.11 (PRKACB protein, human); EC 3.1.- (CNOT6L protein, human); EC 3.1.- (Ribonucleases); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1530/EJE-17-0702


  3 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29223396
[Au] Autor:Matsuishi YI; Kato H; Masuda K; Yamaza H; Hirofuji Y; Sato H; Wada H; Kiyoshima T; Nonaka K
[Ad] Endereço:Section of Oral Medicine for Children, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
[Ti] Título:Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1.
[So] Source:Biochem Biophys Res Commun;495(2):1655-1660, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Undifferentiated odontogenic epithelium and dental papilla cells differentiate into ameloblasts and odontoblasts, respectively, both of which are essential for tooth development. These differentiation processes involve dramatic functional and morphological changes of the cells. For these changes to occur, activation of mitochondrial functions, including ATP production, is extremely important. In addition, these changes are closely related to mitochondrial fission and fusion, known as mitochondrial dynamics. However, few studies have focused on the role of mitochondrial dynamics in tooth development. The purpose of this study was to clarify this role. We used mouse tooth germ organ cultures and a mouse dental papilla cell line with the ability to differentiate into odontoblasts, in combination with knockdown of the mitochondrial fission factor, dynamin related protein (DRP)1. In organ cultures of the mouse first molar, tooth germ developed to the early bell stage. The amount of dentin formed under DRP1 inhibition was significantly larger than that of the control. In experiments using a mouse dental papilla cell line, differentiation into odontoblasts was enhanced by inhibiting DRP1. This was associated with increased mitochondrial elongation and ATP production compared to the control. These results suggest that DRP1 inhibition accelerates dentin formation through mitochondrial elongation and activation. This raises the possibility that DRP1 might be a therapeutic target for developmental disorders of teeth.
[Mh] Termos MeSH primário: Dentinogênese/fisiologia
Dinaminas/antagonistas & inibidores
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/biossíntese
Ameloblastos/citologia
Ameloblastos/fisiologia
Animais
Diferenciação Celular/genética
Diferenciação Celular/fisiologia
Linhagem Celular
Dinaminas/genética
Dinaminas/fisiologia
Proteínas da Matriz Extracelular/biossíntese
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Dinâmica Mitocondrial/fisiologia
Odontoblastos/citologia
Odontoblastos/fisiologia
Técnicas de Cultura de Órgãos
Fosfoproteínas/biossíntese
Gravidez
RNA Interferente Pequeno/genética
Sialoglicoproteínas/biossíntese
Germe de Dente/citologia
Germe de Dente/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (RNA, Small Interfering); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.5.5 (Dnm1l protein, mouse); EC 3.6.5.5 (Dynamins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE


  4 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28471445
[Au] Autor:Wang L; Zhou F; Zhang P; Wang H; Qu Z; Jia P; Yao Z; Shen G; Li G; Zhao G; Li J; Mao Y; Xie Z; Xu W; Xu Y; Xu Y
[Ad] Endereço:Department of Orthopaedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
[Ti] Título:Human type H vessels are a sensitive biomarker of bone mass.
[So] Source:Cell Death Dis;8(5):e2760, 2017 May 04.
[Is] ISSN:2041-4889
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vascularization is fundamental for bone formation and bone tissue homeostasis. However, in human subjects, a direct molecular relationship has not been identified between angiogenesis and agents that promote bone disease or factors related to age. Osteopenia is a condition in which bone mineral density is lower than normal, and it represents a sign of normal aging. Here we tested whether the type H vessel, which was recently identified as strongly positive for CD31 and Endomucin (CD31 Emcn ) in mice, is an important indicator of aging and osteopenia in human subjects. We found that age-dependent losses of type H vessels in human bone sections conform to the observations in aged mice. The abundance of human type H vessels and osteoprogenitors may be relevant to changes in the skeletal microarchitecture and advanced osteopenia. Furthermore, ovariectomized mice, a widely used model for postmenopausal osteoporosis, exhibited significantly reduced type H vessels accompanied by reduced osteoprogenitors, which is consistent with impaired bone microarchitecture and osteoporosis, suggesting that this feature is an indicator of bone mass independent of aging. More importantly, administration of desferrioxamine led to significantly increased bone mass via enhanced angiogenesis and increased type H vessels in ovariectomized mice. Altogether, these data represent a novel finding that type H vessels are regulated in aged and osteopenia subjects. The abundance of human type H vessels is an early marker of bone loss and represents a potential target for improving bone quality via the induction of type H vessels.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Vasos Sanguíneos/metabolismo
Densidade Óssea/fisiologia
Osso e Ossos/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Envelhecimento
Animais
Vasos Sanguíneos/patologia
Doenças Ósseas Metabólicas/metabolismo
Doenças Ósseas Metabólicas/patologia
Osso e Ossos/diagnóstico por imagem
Osso e Ossos/patologia
Modelos Animais de Doenças
Fêmur/irrigação sanguínea
Fêmur/metabolismo
Fêmur/patologia
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Meia-Idade
Osteoporose/metabolismo
Osteoporose/patologia
Osteoporose/veterinária
Sialoglicoproteínas/metabolismo
Células-Tronco/metabolismo
Células-Tronco/patologia
Tíbia/irrigação sanguínea
Tíbia/metabolismo
Tíbia/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (EMCN protein, human); 0 (Sialoglycoproteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1038/cddis.2017.36


  5 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28958711
[Au] Autor:Xu L; Zhang Z; Sun X; Wang J; Xu W; Shi L; Lu J; Tang J; Liu J; Su X
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Soochow University Medical College, Suzhou 215123, China. Electronic address: xulan@suda.edu.cn.
[Ti] Título:Glycosylation status of bone sialoprotein and its role in mineralization.
[So] Source:Exp Cell Res;360(2):413-420, 2017 Nov 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. However, the structural details of its different glycosylation linkages have not been well studied and their functions in bone homeostasis are not clear. Previous studies suggested that the O-glycans, but not the N-glycans on BSP, are highly sialylated. Herein, we employed tandem mass spectrometry (MS/MS) to demonstrate that the N-glycanson the recombinant human integrin binding sialoprotein (rhiBSP) are also enriched in sialic acids (SAs) at their termini. We also identified multiple novel sites of N-glycan modification. Treatment of rhiBSP enhances osteoblast differentiation and mineralization of MC3T3-E1 cells and this effect could be partially reversed by efficient enzymatic removal of its N-glycans. Removal of all terminal SAs has a greater effect in reversing the effect of rhiBSP on osteogenesis, especially on mineralization, suggesting that sialylation at the termini of both N-glycans and O-glycans plays an important role in this regulation. Moreover, BSP-conjugated SAs may affect mineralization via ERK activation of VDR expression. Collectively, our results identified novel N-glycans enriched in SAs on the rhiBSP and demonstrated that SAs at both N- and O-glycans are important for BSP regulation of osteoblast differentiation and mineralization in vitro.
[Mh] Termos MeSH primário: Osso e Ossos/metabolismo
Calcificação Fisiológica
Osteoblastos/metabolismo
Sialoglicoproteínas/metabolismo
[Mh] Termos MeSH secundário: Animais
Metabolismo dos Carboidratos
Sequência de Carboidratos
Linhagem Celular
Glicosilação
Camundongos
Polissacarídeos/metabolismo
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 0 (Sialoglycoproteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE


  6 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28842487
[Au] Autor:Song K; Fu J; Song J; Herzog BH; Bergstrom K; Kondo Y; McDaniel JM; McGee S; Silasi-Mansat R; Lupu F; Chen H; Bagavant H; Xia L
[Ad] Endereço:From the Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104.
[Ti] Título:Loss of mucin-type -glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney.
[So] Source:J Biol Chem;292(40):16491-16497, 2017 Oct 06.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The kidney's filtration activity is essential for removing toxins and waste products from the body. The vascular endothelial cells of the glomerulus are fenestrated, flattened, and surrounded by podocytes, specialized cells that support glomerular endothelial cells. Mucin-type core 1-derived glycans ( -glycans) are highly expressed on both glomerular capillary endothelial cells and their supporting podocytes, but their biological role is unclear. Biosynthesis of core 1-derived -glycans is catalyzed by the glycosyltransferase core 1 ß1,3-galactosyltransferase (C1galt1). Here we report that neonatal or adult mice with inducible deletion of ( ) exhibit spontaneous proteinuria and rapidly progressing glomerulosclerosis. Ultrastructural analysis of the glomerular filtration barrier components revealed that loss of -glycans results in altered podocyte foot processes. Further analysis indicated that -glycan is essential for the normal signaling function of podocalyxin, a podocyte foot process-associated glycoprotein. Our results reveal a new function of -glycosylation in the integrity of the glomerular filtration barrier.
[Mh] Termos MeSH primário: Galactosiltransferases/metabolismo
Mucinas
Podócitos/metabolismo
Polissacarídeos/metabolismo
Sialoglicoproteínas/metabolismo
Transdução de Sinais/fisiologia
[Mh] Termos MeSH secundário: Animais
Galactosiltransferases/genética
Camundongos
Camundongos Knockout
Polissacarídeos/genética
Sialoglicoproteínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucins); 0 (Polysaccharides); 0 (Sialoglycoproteins); 0 (podocalyxin); EC 2.4.1.- (C1galt1 protein, mouse); EC 2.4.1.- (Galactosyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.798512


  7 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28796952
[Au] Autor:Mangione F; EzEldeen M; Bardet C; Lesieur J; Bonneau M; Decup F; Salmon B; Jacobs R; Chaussain C; Opsahl-Vital S
[Ad] Endereço:1 EA 2496 Laboratory Orofacial Pathologies, Imagery and Biotherapies, Dental School and Life imaging Platform (PIV), University Paris Descartes Sorbonne Paris Cité, Montrouge, France.
[Ti] Título:Implanted Dental Pulp Cells Fail to Induce Regeneration in Partial Pulpotomies.
[So] Source:J Dent Res;96(12):1406-1413, 2017 Nov.
[Is] ISSN:1544-0591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell-based partial pulp regeneration is one of the promising approaches to obtain newly formed functional dentin-pulp complex. It relies on the preservation of the healthy tissue while regenerating the damaged pulp. The aim of this study was to investigate whether this regenerative process could be achieved by implanting porcine dental pulp cells (pDPCs) in pulp defects in the minipig. By split-mouth model, self-assembling injectable nanopeptide hydrogel, with and without pDPCs, was implanted after cameral pulpotomy in premolars and molars. At day 21 after surgery, 3-dimensional morphometric characterization, Masson's trichrome staining, and immunolabeling for DSP and BSP (dentin sialoprotein and bone sialoprotein) were performed on treated teeth. This study demonstrated no pulp regeneration but systematic reparative dentinogenesis. In fact, regardless of the presence of pDPCs in the scaffold, an osteodentin bridge-the microarchitecture of which significantly differed from the native dentin-was systematically obtained. Furthermore, the presence of pDPCs significantly affected the microstructure of the dentin bridges. In the radicular area of each treated tooth, hyperemia in the remaining pulp and external root resorptions were observed. Under the conditions tested in this work, pulp regeneration was not achieved, which highlights the need of further investigations to develop favorable regenerative microenvironment.
[Mh] Termos MeSH primário: Polpa Dentária/citologia
Pulpotomia
Regeneração
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Dentina Secundária/fisiologia
Proteínas da Matriz Extracelular/análise
Hidrogéis
Sialoproteína de Ligação à Integrina/análise
Fosfoproteínas/análise
Sialoglicoproteínas/análise
Coloração e Rotulagem
Suínos
Porco Miniatura
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Hydrogels); 0 (Integrin-Binding Sialoprotein); 0 (Phosphoproteins); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1177/0022034517725523


  8 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28707474
[Au] Autor:Lindström I; Dogan J
[Ad] Endereço:Department of Biochemistry and Biophysics, Stockholm University , 10691 Stockholm, Sweden.
[Ti] Título:Native Hydrophobic Binding Interactions at the Transition State for Association between the TAZ1 Domain of CBP and the Disordered TAD-STAT2 Are Not a Requirement.
[So] Source:Biochemistry;56(32):4145-4153, 2017 Aug 15.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A significant fraction of the eukaryotic proteome consists of proteins that are either partially or completely disordered under native-like conditions. Intrinsically disordered proteins (IDPs) are common in protein-protein interactions and are involved in numerous cellular processes. Although many proteins have been identified as disordered, much less is known about the binding mechanisms of the coupled binding and folding reactions involving IDPs. Here we have analyzed the rate-limiting transition state for binding between the TAZ1 domain of CREB binding protein and the intrinsically disordered transactivation domain of STAT2 (TAD-STAT2) by site-directed mutagenesis and kinetic experiments (Φ-value analysis) and found that the native protein-protein binding interface is not formed at the transition state for binding. Instead, native hydrophobic binding interactions form late, after the rate-limiting barrier has been crossed. The association rate constant in the absence of electrostatic enhancement was determined to be rather high. This is consistent with the Φ-value analysis, which showed that there are few or no obligatory native contacts. Also, linear free energy relationships clearly demonstrate that native interactions are cooperatively formed, a scenario that has usually been observed for proteins that fold according to the so-called nucleation-condensation mechanism. Thus, native hydrophobic binding interactions at the rate-limiting transition state for association between TAD-STAT2 and TAZ1 are not a requirement, which is generally in agreement with previous findings on other IDP systems and might be a common mechanism for IDPs.
[Mh] Termos MeSH primário: Proteínas Intrinsicamente Desordenadas/química
Modelos Químicos
Fragmentos de Peptídeos/química
Fator de Transcrição STAT2/química
Sialoglicoproteínas/química
[Mh] Termos MeSH secundário: Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Proteínas Intrinsicamente Desordenadas/genética
Proteínas Intrinsicamente Desordenadas/metabolismo
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Ligação Proteica
Domínios Proteicos
Fator de Transcrição STAT2/genética
Fator de Transcrição STAT2/metabolismo
Sialoglicoproteínas/genética
Sialoglicoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Intrinsically Disordered Proteins); 0 (Peptide Fragments); 0 (STAT2 Transcription Factor); 0 (STAT2 protein, human); 0 (Sialoglycoproteins); 0 (bone sialoprotein (35-62), human)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00428


  9 / 8204 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28648376
[Au] Autor:Benoit YD; Mitchell RR; Risueño RM; Orlando L; Tanasijevic B; Boyd AL; Aslostovar L; Salci KR; Shapovalova Z; Russell J; Eguchi M; Golubeva D; Graham M; Xenocostas A; Trus MR; Foley R; Leber B; Collins TJ; Bhatia M
[Ad] Endereço:McMaster Stem Cell and Cancer Research Institute, Faculty of Health Sciences, McMaster University, 1280 Main Street West, MDCL 5029, Hamilton, ON L8S 4L8, Canada.
[Ti] Título:Sam68 Allows Selective Targeting of Human Cancer Stem Cells.
[So] Source:Cell Chem Biol;24(7):833-844.e9, 2017 Jul 20.
[Is] ISSN:2451-9456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Targeting of human cancer stem cells (CSCs) requires the identification of vulnerabilities unique to CSCs versus healthy resident stem cells (SCs). Unfortunately, dysregulated pathways that support transformed CSCs, such as Wnt/ß-catenin signaling, are also critical regulators of healthy SCs. Using the ICG-001 and CWP family of small molecules, we reveal Sam68 as a previously unappreciated modulator of Wnt/ß-catenin signaling within CSCs. Disruption of CBP-ß-catenin interaction via ICG-001/CWP induces the formation of a Sam68-CBP complex in CSCs that alters Wnt signaling toward apoptosis and differentiation induction. Our study identifies Sam68 as a regulator of human CSC vulnerability.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Proteínas de Ligação a DNA/metabolismo
Células-Tronco Neoplásicas/metabolismo
Fragmentos de Peptídeos/metabolismo
Proteínas de Ligação a RNA/metabolismo
Sialoglicoproteínas/metabolismo
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/antagonistas & inibidores
Proteínas Adaptadoras de Transdução de Sinal/genética
Adulto
Idoso
Animais
Apoptose/efeitos dos fármacos
Compostos Azabicíclicos/farmacologia
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Neoplasias do Colo/metabolismo
Neoplasias do Colo/patologia
Proteínas de Ligação a DNA/antagonistas & inibidores
Proteínas de Ligação a DNA/genética
Feminino
Seres Humanos
Leucemia Mieloide Aguda/metabolismo
Leucemia Mieloide Aguda/patologia
Masculino
Camundongos
Camundongos Endogâmicos NOD
Meia-Idade
Células-Tronco Neoplásicas/citologia
Células-Tronco Neoplásicas/transplante
Organofosfatos/farmacologia
Fragmentos de Peptídeos/antagonistas & inibidores
Fragmentos de Peptídeos/genética
Proteínas Proto-Oncogênicas c-myc/metabolismo
Pirimidinonas/farmacologia
Interferência de RNA
Proteínas de Ligação a RNA/antagonistas & inibidores
Proteínas de Ligação a RNA/genética
Sialoglicoproteínas/antagonistas & inibidores
Sialoglicoproteínas/genética
Sumoilação/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
Via de Sinalização Wnt/efeitos dos fármacos
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Azabicyclo Compounds); 0 (Bridged Bicyclo Compounds, Heterocyclic); 0 (CWP232228); 0 (DNA-Binding Proteins); 0 (ICG 001); 0 (KHDRBS1 protein, human); 0 (Organophosphates); 0 (Peptide Fragments); 0 (Proto-Oncogene Proteins c-myc); 0 (Pyrimidinones); 0 (RNA-Binding Proteins); 0 (Sialoglycoproteins); 0 (beta Catenin); 0 (bone sialoprotein (35-62), human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


  10 / 8204 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28521605
[Au] Autor:Ogata M; Koizumi A; Otsubo T; Ikeda K; Sakamoto M; Aita R; Kato T; Park EY; Yamanaka T; Hidari KIPJ
[Ad] Endereço:a Department of Chemistry and Biochemistry, National Institute of Technology , Fukushima College , Iwaki , Japan.
[Ti] Título:Chemoenzymatic synthesis and characterization of N-glycolylneuraminic acid-carrying sialoglycopolypeptides as effective inhibitors against equine influenza virus hemagglutination.
[So] Source:Biosci Biotechnol Biochem;81(8):1520-1528, 2017 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of novel sialoglycopolypeptides carrying N-glycolylneuraminic acid (Neu5Gc)-containing trisaccharides having α(2 â†’ 3)- and α(2 â†’ 6)-linkages in the side chains of γ-polyglutamic acid (γ-PGA) were designed as competitive inhibitors against equine influenza viruses (EIV), which critically recognize the Neu5Gc residue for receptor binding. Using horse red blood cells (HRBC) we successfully evaluated the binding activity of the multivalent Neu5Gc ligands to both equine and canine influenza viruses in the hemagglutination inhibition (HI) assay. Our findings show the multivalent α2,3-linked Neu5Gc-ligands (3a-c and 7) selectively inhibit hemagglutination mediated by both influenza viruses and display a strong inhibitory activity. Our results indicate that the multivalent Neu5Gc-ligands can be used as novel probes to elucidate the mechanism of infection/adhesion of Neu5Gc-binding influenza viruses.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Hemaglutinação/efeitos dos fármacos
Orthomyxoviridae/efeitos dos fármacos
Sialoglicoproteínas/farmacologia
Sialiltransferases/química
[Mh] Termos MeSH secundário: Animais
Antivirais/química
Antivirais/metabolismo
Ligação Competitiva
Bombyx
Sequência de Carboidratos
Clonagem Molecular
Cães
Eritrócitos/efeitos dos fármacos
Eritrócitos/virologia
Expressão Gênica
Testes de Inibição da Hemaglutinação
Hemolinfa/química
Cavalos
Seres Humanos
Ácidos Neuramínicos/química
Nucleopolyhedrovirus/genética
Nucleopolyhedrovirus/metabolismo
Ácido Poliglutâmico/análogos & derivados
Ácido Poliglutâmico/química
Ácido Poliglutâmico/metabolismo
Ratos
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Sialoglicoproteínas/biossíntese
Sialoglicoproteínas/química
Sialiltransferases/genética
Sialiltransferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Neuraminic Acids); 0 (Recombinant Proteins); 0 (Sialoglycoproteins); 0 (poly(gamma-glutamic acid)); 1113-83-3 (N-glycolylneuraminic acid); 25513-46-6 (Polyglutamic Acid); EC 2.4.99.- (Sialyltransferases); EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1325315



página 1 de 821 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde