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[PMID]:28386549
[Au] Autor:Pérez-Alvarado C; Gómez C; Reyes M; García M; Pérez E; Pérez de la Mora C; Sanchez V; Pérez Ishiwara DG
[Ad] Endereço:ENMH, Instituto Politécnico Nacional, Ciudad de México, Mexico.
[Ti] Título:Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model.
[So] Source:Biomed Res Int;2017:1832853, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To evaluate the anti-inflammatory properties of Dialyzable Leukocyte Extract (DLE) in a murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Histopathological characterization, prostatein Enzyme-Linked Immunosorbent Assay, and immunohistochemical analysis for CD45, TNF- , IFN- , IL-6, IL-17, and IL-4 molecules were done in prostatic Wistar rats treated with DLE, placebo, or Dexamethasone. Histopathological analysis of animals induced to prostatitis showed inflammatory infiltrate, mainly constituted by leucocytes and mast cells as well as Benign Prostatic Hyperplasia. Serum prostatein concentrations were 14 times higher than those displayed by healthy animals. After DLE and Dexamethasone treatments, the inflammatory infiltrate decreased; the tissue morphology was similar to that of a normal prostate, and the prostatein decreased to the basal levels of healthy animals. DLE treatment produced a decreased expression of the cell surface marker CD45 and the proinflammatory cytokines TNF- , IFN- , IL-6, and IL-17. On the other hand, the expression of anti-inflammatory cytokine IL-4 increased in both the Dexamethasone and DLE groups. DLE is able to modulate the inflammatory response in Experimental Autoimmune Prostatitis (EAP).
[Mh] Termos MeSH primário: Doenças Autoimunes/tratamento farmacológico
Inflamação/tratamento farmacológico
Prostatite/tratamento farmacológico
Fator de Transferência/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Doenças Autoimunes/sangue
Doenças Autoimunes/patologia
Dexametasona
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Inflamação/sangue
Inflamação/patologia
Interleucina-17/biossíntese
Interleucina-4/biossíntese
Interleucina-6/biossíntese
Antígenos Comuns de Leucócito/biossíntese
Masculino
Camundongos
Prostateína/sangue
Hiperplasia Prostática/sangue
Hiperplasia Prostática/tratamento farmacológico
Hiperplasia Prostática/patologia
Prostatite/sangue
Prostatite/patologia
Ratos
Fator de Necrose Tumoral alfa/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-17); 0 (Interleukin-6); 0 (Prostatein); 0 (Transfer Factor); 0 (Tumor Necrosis Factor-alpha); 207137-56-2 (Interleukin-4); 7S5I7G3JQL (Dexamethasone); EC 3.1.3.48 (Leukocyte Common Antigens); EC 3.1.3.48 (Ptprc protein, rat)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1155/2017/1832853


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[PMID]:28189890
[Au] Autor:Cardoso FM; Tomkova M; Petrovajova D; Bubanova M; Ragac O; Hornakova T
[Ad] Endereço:R&D Department, Imuna Pharm a. s., Jarková 17, 082 22 Sarisské Michalany, Slovak Republic. Electronic address: miguel.lopez.cardoso@gmail.com.
[Ti] Título:New and cost effective cell-based assay for Dialyzed Leukocyte Extract (DLE)-induced Jurkat cells proliferation under azathioprine treatment.
[So] Source:J Pharm Biomed Anal;138:100-108, 2017 May 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The human Dialyzed Leukocyte Extract (DLE) is a heterogeneous mix of oligopeptides of <10kDa, extracted from leukocytes of healthy donors. There is significant clinical evidence of improvement using DLE during treatment of allergies, cancer,immunodeficiencies, and in mycotic and viral infections. Nevertheless, the DLE exact nature and mechanism of action have been elusive for more than 50 years. DLE biological activity testing is necessary in DLE production and quality control. Both in vitro and in vivo assays exist: E-rosette test, induction of delayed type hypersensitivity in mice, leukocyte migration and IFN-γ secretion. The animal-origin materials and in vivo assays convey a considerable logistic, ethic and economic burden, meanwhile the available in vitro assays have been reported with limited reproducibility and sometimes contradictory results. Here we are reporting a new DLE biological activity cell-based assay. The A20 and Jurkat cell lines were treated with (+Aza) or without (-Aza) azathioprine, DLE (+DLE) or both (+Aza/+DLE). After 72h, the cell proliferation was analyzed by the MTT or BrdU incorporation assays. In +Aza/+DLE treated cells, we observed a significant higher proliferation, when compared with +Aza/-DLE. In the absence of Aza, cells did not present any proliferation difference between -DLE or +DLE treatments. Both assays, MTT and BrdU showed similar results, being the MTT test more cost effective and we select it for validation as DLE biological assay using Jurkat cells only. We tested three different lyophilized DLE batches and we found consistent results with acceptable assay reproducibility and linearity. The DLE capacity for rescuing Jurkat cell proliferation during +Aza treatment was consistent using different liquid and lyophilized DLE batches, presenting also consistent chromatographic profiles. Finally, DLE treatment in Jurkat cells did not result into significant IL-2 of IFN-γ secretion, and known lymphocyte proliferative drugs failed to rescue Jurkat cells viability in presence of +Aza, as +DLE treatment did in our MTT assay. In conclusion, our new cell-based MTT assay has excellent DLE biological activity consistency, robustness and is cost effective, presenting important advantages over previous DLE activity in vitro and in vivo assays.
[Mh] Termos MeSH primário: Azatioprina/farmacologia
Proliferação Celular/efeitos dos fármacos
Células Jurkat/efeitos dos fármacos
Células Jurkat/fisiologia
Leucócitos/efeitos dos fármacos
Leucócitos/fisiologia
Fator de Transferência/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Análise Custo-Benefício/métodos
Seres Humanos
Camundongos
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transfer Factor); MRK240IY2L (Azathioprine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE


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[PMID]:27847830
[Au] Autor:Ramírez-Ramírez D; Vadillo E; Arriaga-Pizano LA; Mayani H; Estrada-Parra S; Velasco-Velázquez MA; Pérez-Tapia SM; Pelayo R
[Ad] Endereço:Oncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, Mexico; Department of Immunology, National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Sa
[Ti] Título:Early Differentiation of Human CD11c NK Cells with T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts.
[So] Source:J Immunol Res;2016:4097642, 2016.
[Is] ISSN:2314-7156
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:Reconstitution of the hematopoietic system during immune responses and immunological and neoplastic diseases or upon transplantation depends on the emergent differentiation of hematopoietic stem/progenitor cells within the bone marrow. Although in the last decade the use of dialyzable leukocyte extracts (DLE) as supportive therapy in both infectious and malignant settings has increased, its activity on the earliest stages of human hematopoietic development remains poorly understood. Here, we have examined the ability of DLE to promote replenishment of functional lymphoid lineages from CD34 cells. Our findings suggest that DLE increases their differentiation toward a conspicuous CD56 CD16 CD11c NK-like cell population endowed with properties such as IFNy production, tumor cell cytotoxicity, and the capability of inducing T lymphocyte proliferation. Of note, long-term coculture controlled systems showed the bystander effect of DLE-stromal cells by providing NK progenitors with signals to overproduce this cell subset. Thus, by direct effect on progenitor cells and through activation and remodeling of the supporting hematopoietic microenvironment, DLE may contribute a robust innate immune response by promoting the emerging lymphopoiesis of functional CD11c NK cells in a partially TLR-related manner. Unraveling the identity and mechanisms of the involved DLE components may be fundamental to advance the NK cell-based therapy field.
[Mh] Termos MeSH primário: Células Matadoras Naturais/efeitos dos fármacos
Células Matadoras Naturais/imunologia
Ativação Linfocitária
Linfopoese
Subpopulações de Linfócitos T/imunologia
Fator de Transferência/farmacologia
[Mh] Termos MeSH secundário: Antígeno CD11c/análise
Células Cultivadas
Técnicas de Cocultura
Células-Tronco Hematopoéticas/fisiologia
Seres Humanos
Imunofenotipagem
Interferon gama/biossíntese
Células Matadoras Naturais/fisiologia
Receptores de Antígenos de Linfócitos T gama-delta
Células Estromais/fisiologia
Subpopulações de Linfócitos T/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD11c Antigen); 0 (Receptors, Antigen, T-Cell, gamma-delta); 0 (Transfer Factor); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE


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[PMID]:27513196
[Au] Autor:Tagami K; Howard BJ; Uchida S
[Ad] Endereço:Office of Biospheric Assessment for Waste Disposal, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology : Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan.
[Ti] Título:The Time-Dependent Transfer Factor of Radiocesium from Soil to Game Animals in Japan after the Fukushima Dai-ichi Nuclear Accident.
[So] Source:Environ Sci Technol;50(17):9424-31, 2016 Sep 06.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since the Fukushima Dai-ichi accident, monitoring of tissues from hunted game animals ensures compliance with the standard food limits for radionuclides in Japan. We quantified the transfer of (137)Cs from contaminated land to game animals using the Aggregated transfer factor (Tag = activity concentration in meat [Bq kg(-1) fw]/amount in soil [Bq m(-2)]) of (137)Cs for Asian black bear, wild boar, sika deer, green pheasant, copper pheasant and wild duck, collected between 2011 and 2015. Open data sources were used from Fukushima, Miyagi, Ibaraki, Tochigi, and Gunma prefectures. Our initially compiled data showed that the maximum reported (137)Cs activity concentration in wild boar after the Fukushima Dai-ichi accident were lower than those reported after the Chernobyl accident. The geometric mean Tag values (m(2)kg(-1) fw) of (137)Cs in 2015 for Asian black bear, wild boar, sika deer and copper pheasant were similar (1.9-5.1) × 10(-3) while those for green pheasant and wild duck were about 1 order of magnitude lower at (1.0-2.2) × 10(-4). Effective half-lives were 1.2-6.9 y except for sika deer and copper pheasant where no decreases were found. In contrast to the Chernobyl accident, no seasonal change occurred in the meat (137)Cs activity concentrations of the wild animals during the study period.
[Mh] Termos MeSH primário: Radioisótopos de Césio
Acidente Nuclear de Fukushima
[Mh] Termos MeSH secundário: Animais
Japão
Monitoramento de Radiação
Solo
Fator de Transferência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cesium Radioisotopes); 0 (Soil); 0 (Transfer Factor)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b03011


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[PMID]:27155835
[Au] Autor:Pérez-Carrera A; Alvarez-Gonçalvez CV; Fernández-Cirelli A
[Ad] Endereço:Instituto de Investigaciones en Producción Animal (INPA), CONICET-UBA, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín N° 280, C1427CWO, Ciudad de Buenos Aires, Argentina.
[Ti] Título:Transference factors as a tool for the estimation of arsenic milk concentration.
[So] Source:Environ Sci Pollut Res Int;23(16):16329-35, 2016 Aug.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The Chaco Pampean Plain of central Argentina represents one of the largest regions with high levels of arsenic (As) in groundwater. The aim of this study was the assessment of a biotransference factor (BTF) as a tool for the estimation of As concentration in cow's milk from As drinking water concentration. Total As content in livestock drinking water, soil, forage, and milk was determined in farms located in an area of high As groundwater, in order to analyze the relation between As uptake and its transfer to milk. The concentrations of As in milk ranged from 0.5 to 8.0 µg/L. From the results obtained, drinking water may be considered the main source of exposure to As, and the biotransference factor for milk ranges from 1.5 × 10(-5) to 4.3 × 10(-4). Therefore, BTF provides a simple tool for the estimation of arsenic levels in milk through the As livestock drinking water content.
[Mh] Termos MeSH primário: Arsênico/análise
Monitoramento Ambiental/métodos
Poluentes Ambientais/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Argentina
Bovinos
Água Potável/química
Feminino
Água Subterrânea/química
Fator de Transferência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water); 0 (Environmental Pollutants); 0 (Transfer Factor); N712M78A8G (Arsenic)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160509
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-6731-0


  6 / 1061 MEDLINE  
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Corrêa, B
[PMID]:27098310
[Au] Autor:Acosta P; Pérez N; Pérez E; Correa B; Pérez C; Gómez C; Sánchez V; Pérez DG
[Ad] Endereço:a Laboratory of Molecular Biomedicine , National School of Medicine and Homeopathy (ENMH), National Polytechnic Institute (IPN) , Mexico City , Mexico.
[Ti] Título:Anti-inflammatory effect of dialysable leucocyte extract in a rat model of osteoarthritis: histopathological and molecular characterization.
[So] Source:Scand J Rheumatol;45(6):528-535, 2016 Nov.
[Is] ISSN:1502-7732
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To evaluate the effect of dialysable leucocyte extract (DLE) on pro- and anti-inflammatory profiles in a rat model of osteoarthritis (OA). METHOD: Forty-eight male Wistar rats were divided into three groups: normal rats without treatment, OA rats treated with placebo, and OA rats treated with DLE. After treatment, the animals were killed to obtain cartilage for histological analysis and to determine the expression of pro- and anti-inflammatory cytokines by reverse transcription multiplex polymerase chain reaction (RT-MPCR) and immunohistofluorescence analyses. RESULTS: Histological analysis revealed that OA cartilage from rats treated with DLE displayed similar characteristics to non-OA cartilage from the control group. The OA cartilage treated with placebo showed alterations in the cellular architecture and in chondrocyte cluster formation. Analysis of cytokine expression by RT-MPCR showed that OA cartilage from DLE-treated rats expressed platelet-derived growth factor (PDGF), interferon (IFN)-γ, and fibroblast growth factor (FGF)-2, similar to non-OA cartilage from the control group. However, OA cartilage from rats treated with placebo expressed interleukin (IL)-1, PDGF, and I kappa B (IκB). Confocal immunodetection of FGF-2, PDGF, and non-phosphorylated IκB showed that they were distributed in the cytoplasm of most chondrocytes in OA cartilage from DLE-treated rats whereas no nuclear factor kappa B (NF-κB) expression was observed in the nuclei. Instead, in OA cartilage from the placebo group, only weak FGF-2 staining was observed, PDGF and IκB were not detected, and NF-κB was strongly observed in both cytoplasm and nuclei. CONCLUSIONS: Our findings suggest that DLE treatment modifies the OA process, promoting the expression of anti-inflammatory cytokines and diminishing the inflammatory effects, avoiding the nuclear translocation of NF-κB in chondrocytes.
[Mh] Termos MeSH primário: Artrite Experimental/tratamento farmacológico
Osteoartrite/tratamento farmacológico
Fator de Transferência/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Artrite Experimental/metabolismo
Artrite Experimental/patologia
Condrócitos/efeitos dos fármacos
Condrócitos/metabolismo
Citocinas/metabolismo
Avaliação Pré-Clínica de Medicamentos
Fator 2 de Crescimento de Fibroblastos/metabolismo
Proteínas I-kappa B/metabolismo
Masculino
NF-kappa B/metabolismo
Osteoartrite/metabolismo
Osteoartrite/patologia
Fator de Crescimento Derivado de Plaquetas/metabolismo
Ratos Wistar
Fator de Transferência/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (I-kappa B Proteins); 0 (NF-kappa B); 0 (Platelet-Derived Growth Factor); 0 (Transfer Factor); 103107-01-3 (Fibroblast Growth Factor 2)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE


  7 / 1061 MEDLINE  
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[PMID]:24825752
[Au] Autor:Zhou J; Kong C; Wang X; Shao J; Feng L; Zhang Z
[Ad] Endereço:Clinical Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong, People's Republic of China.
[Ti] Título:Preparation and identification of transfer factor specific to Staphylococcus aureus in vitro.
[So] Source:Biotechnol Appl Biochem;62(1):112-20, 2015 Jan-Feb.
[Is] ISSN:1470-8744
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of the study was to explore the methods for preparing transfer factor specific to Staphylococcus aureus (SA-STF) in vitro. Under the optimum conditions, the spleen cells of rabbits were immunized with SA in vitro to prepare SA-STF, and the immune activities were identified with the phagocytosis and sterilization, skin delayed-type hypersensitivity, and immune protection tests. The concentration of polypeptide was 2.26 ± 0.27 mg/mL, and ribose was 0.684 ± 0.094 mg/mL. The phagocytosis and sterilization rates of the STF group were 70.9 ± 12.4% and 62.1 ± 12.2%, respectively, and compared with the non-specific transfer factor (NTF) group, there were no significant differences (P = 0.074 and 0.069, respectively). The skin was inflamed and marked nodules formed at the injection site in the mice of the STF group rather than the NTF and control groups. The survival rate of the STF-1 group was significantly higher than the survival rates of the STF-2 (P = 0.024) and NTF groups (P = 0.016). SA-STF was prepared and characterized successfully in vitro, and it probably is a biological candidate for therapy or adjuvant therapy for diseases caused by Staphylococcus aureus.
[Mh] Termos MeSH primário: Staphylococcus aureus/imunologia
Fator de Transferência/imunologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Químicos
Hipersensibilidade Tardia/imunologia
Camundongos
Fagocitose
Coelhos
Pele/imunologia
Especificidade da Espécie
Fator de Transferência/efeitos adversos
Fator de Transferência/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Transfer Factor)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140515
[St] Status:MEDLINE
[do] DOI:10.1002/bab.1241


  8 / 1061 MEDLINE  
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[PMID]:25629265
[Au] Autor:Zajícová A; Javorková E; Trosan P; Chudícková M; Krulová M; Holán V
[Ad] Endereço:Department of Transplantation Immunology, Institute of Experimental Medicine, AS CR, v. v. i., Prague, Czech Republic.
[Ti] Título:A low-molecular-weight dialysable leukocyte extract selectively enhances development of CD4⁺RORγt⁺ T cells and IL-17 production.
[So] Source:Folia Biol (Praha);60(6):253-60, 2014.
[Is] ISSN:0015-5500
[Cp] País de publicação:Czech Republic
[La] Idioma:eng
[Ab] Resumo:A low-molecular-weight (under 10 kDa) dialysable leukocyte extract (called transfer factor, TF) has been shown to be a prospective substance to improve or modulate immune response in autoimmunity, inflammation, infectious diseases or cancers. However, the use of TF has been limited by the absence of any data on the mechanism of its action. Here we show that TF prepared from peripheral blood leukocytes of healthy human donors displays multiple regulatory effects on individual parameters of the immune system. TF decreases proliferation of T and B lymphocytes and partially alters the production of cytokines and nitric oxide by activated macrophages. TF also inhibits production of T helper 1 (Th1) cytokines interleukin 2 (IL-2) and interferon γ, slightly stimulates production of Th2 cytokine IL-10 and considerably enhances the secretion of IL-17 by activated mouse spleen T cells. At the molecular level, TF enhances expression of genes for transcription factor RORγt and for IL-17. The enhanced expression of the RORgt gene corresponds with an increase in the number of RORγt⁺CD4⁺ Th17 cells and with enhanced IL-17 production. In contrast, the expression of the Foxp3 gene and the proportion of CD4⁺CD25⁺Foxp3⁺ regulatory T cells are not significantly changed in the presence of TF. These results suggest that the activation of pro-inflammatory Th17 cells, which have multiple immunoregulatory properties, could be the main mechanism of the immunomodulatory action of a low-molecular-weight leukocyte extract.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Linfócitos T CD4-Positivos/efeitos dos fármacos
Interleucina-17/biossíntese
Subpopulações de Linfócitos/efeitos dos fármacos
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/biossíntese
Fator de Transferência/farmacologia
[Mh] Termos MeSH secundário: Animais
Linfócitos B/efeitos dos fármacos
Linfócitos T CD4-Positivos/metabolismo
Divisão Celular/efeitos dos fármacos
Células Cultivadas
Concanavalina A/farmacologia
Avaliação Pré-Clínica de Medicamentos
Fatores de Transcrição Forkhead/biossíntese
Fatores de Transcrição Forkhead/genética
Regulação da Expressão Gênica/efeitos dos fármacos
Interferon gama/biossíntese
Interferon gama/genética
Interleucina-17/genética
Interleucinas/biossíntese
Interleucinas/genética
Ativação Linfocitária/efeitos dos fármacos
Subpopulações de Linfócitos/metabolismo
Macrófagos Peritoneais/efeitos dos fármacos
Macrófagos Peritoneais/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Peso Molecular
Óxido Nítrico/biossíntese
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/análise
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/genética
Reação em Cadeia da Polimerase em Tempo Real
Baço/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Forkhead Transcription Factors); 0 (Foxp3 protein, mouse); 0 (Interleukin-17); 0 (Interleukins); 0 (Nuclear Receptor Subfamily 1, Group F, Member 3); 0 (Transfer Factor); 11028-71-0 (Concanavalin A); 31C4KY9ESH (Nitric Oxide); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150129
[Lr] Data última revisão:
150129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150129
[St] Status:MEDLINE


  9 / 1061 MEDLINE  
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[PMID]:24678833
[Au] Autor:Zhang Y; Liu B; Li X; Ouyang Z; Huang L; Hong Y; Zhang H; Li D; Song F
[Ti] Título:The de novo biosynthesis of vitamin B6 is required for disease resistance against Botrytis cinerea in tomato.
[So] Source:Mol Plant Microbe Interact;27(7):688-99, 2014 Jul.
[Is] ISSN:0894-0282
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vitamin B6 (VB6), an essential cofactor for numerous metabolic enzymes, has recently been shown to act as a potent antioxidant and play important roles in developmental processes and stress responses. However, little is known about the possible function of VB6 in plant disease resistance response against pathogen infection. In the present study, we explored the possible involvement of VB6 in defense response against Botrytis cinerea through functional analysis of tomato VB6 biosynthetic genes. Three de novo VB6 biosynthetic genes (SlPDX1.2, SlPDX1.3, and SlPDX2) and one salvage pathway gene (SlSOS4) were identified and the SlPDX1.2, SlPDX1.3, and SlPDX2 genes were shown to encode functional enzymes involved in de novo biosynthesis of VB6, as revealed by complementation of the VB6 prototrophy in yeast snz1 and sno1 mutants. Expression of SlPDX1.2, SlPDX1.3, and SlSOS4 genes was induced by infection with B. cinerea. Virus-induced gene silencing-mediated knockdown of SlPDX1.2 or SlPDX1.3 but not SlPDX2 and SlSOS4 led to increased severity of disease caused by B. cinerea, indicating that the VB6 de novo biosynthetic pathway but not the salvage pathway is involved in tomato defense response against B. cinerea. Furthermore, the SlPDX1.2- and SlPDX1.3-silenced tomato plants exhibited reduced levels of VB6 contents and reactive oxygen species scavenging capability, increased levels of superoxide anion and H2O2 generation, and increased activity of superoxide dismutase after infection by B. cinerea. Our results suggest that VB6 and its de novo biosynthetic pathway play important roles in regulation of defense response against B. cinerea through modulating cellular antioxidant capacity.
[Mh] Termos MeSH primário: Botrytis/imunologia
Lycopersicon esculentum/metabolismo
Doenças das Plantas/microbiologia
Vitamina B 6/biossíntese
[Mh] Termos MeSH secundário: Agrobacterium
Deleção de Genes
Regulação da Expressão Gênica de Plantas
Doenças das Plantas/imunologia
Folhas de Planta
Espécies Reativas de Oxigênio
Simbiose
Fator de Transferência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 0 (Transfer Factor); 8059-24-3 (Vitamin B 6)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140530
[Lr] Data última revisão:
140530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140401
[St] Status:MEDLINE
[do] DOI:10.1094/MPMI-01-14-0020-R


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[PMID]:24369255
[Au] Autor:Xu Y; Zhang Q; Zhan X; Xie D; Dai G; Yang H
[Ad] Endereço:Jiangxi Academy of Medical Sciences, Nanchang 330006, China. E-mail: ypxu430@163.com.
[Ti] Título:[Preparation and immunological evaluation of oral solution of egg yolk-derived hepatitis B virus-specific transfer factor].
[So] Source:Nan Fang Yi Ke Da Xue Xue Bao;33(12):1827-30, 2013 Dec.
[Is] ISSN:1673-4254
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To prepare the oral solution of egg yolk hepatitis B virus (HBV)-specific transfer factor (EYHBV-TF) and evaluate its immunological activity as an immune regulator against hepatitis B. METHODS: From hens immunized with the Hepatitis B vaccine the egg yolk was isolated to extract the specific transfer factor EYHBV-TF, and its physicochemical properties were examined. Leukocyte adhesion inhibition test (LAI) was performed to detect the immunogenic activity of EYHBV-TF. The solution of EYHBV-TF was then administered orally in normal mice, and the specific cellular immune activity induced was assayed with delayed type skin hypersensitivity test (DTH), with the non-specific immune activity assessed with immune organ index. The immune responses induced by oral EYHBV-STF solution were compared with those by EYHBV-STF injection and by different dosages (injection and oral) of porcine spleen HBV-specific transfer factor (PSHBV-STF), porcine spleen nonspecific transfer factor, and egg yolk extracts from non-immunized hens. RESULTS: The prepared EYHBV-STF oral solution, which met the standards for biological products, could inhibit leukocyte adhesion in vitro and significantly enhance mouse foot pad swelling, demonstrating its capability of transferring antigen-specific delayed type hypersensitivity reactions to naive recipient. EYHBV-STF oral solution also significantly improved the immune organ index in mice (P<0 01) with similar effects to those caused by EYHBV-STF injections and by PSHBV-STF injection and oral solution. CONCLUSION: Orally administered EYHBV-STF and EYHBV-STF injection both possess hepatitis B antigen-specific cellular immune activity and can significantly enhance specific cellular immune responses.
[Mh] Termos MeSH primário: Vírus da Hepatite B/efeitos dos fármacos
Hepatite B/tratamento farmacológico
Imunidade Celular
Fator de Transferência/farmacologia
[Mh] Termos MeSH secundário: Animais
Galinhas
Gema de Ovo/química
Antígenos da Hepatite B
Imunização
Camundongos
Suínos
Fator de Transferência/administração & dosagem
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hepatitis B Antigens); 0 (Transfer Factor)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:131226
[Lr] Data última revisão:
131226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131227
[St] Status:MEDLINE



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