[PMID]: | 10502070 |
[Au] Autor: | Jenkinson KM; Morgan JM; Furness JB; Southwell BR |
[Ad] Endereço: | Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria 3052, Australia, k.Jenkinson@anatomy.unimelb.edu.au |
[Ti] Título: | Neurons bearing NK(3) tachykinin receptors in the guinea-pig ileum revealed by specific binding of fluorescently labelled agonists. |
[So] Source: | Histochem Cell Biol;112(3):233-46, 1999 Sep. |
[Is] ISSN: | 0948-6143 |
[Cp] País de publicação: | Germany |
[La] Idioma: | eng |
[Ab] Resumo: | The localisation of NK(3) tachykinin receptors in guinea-pig ileum was studied using the fluorescently labelled agonists, Cy3. 5-neurokinin A and Cy3.5-kassinin. Binding to nerve cell bodies in the myenteric and submucosal plexuses was visualised using confocal microscopy. Binding to NK(1) receptors was blocked by the NK(1) receptor antagonist, CP-99994. NK(3) receptors, demonstrated by binding in the presence of CP-99994, occurred in 72% of myenteric and 38% of submucosal neurons. Colocalisation with other markers was examined to deduce the classes of neurons with NK(3) receptors. In myenteric ganglia, NK(3) receptors were present on the following: 73% of calbindin-immunoreactive (IR) intrinsic primary afferent neurons, 63% of calretinin-IR excitatory motor neurons and ascending interneurons, 63% of nitric oxide synthase-IR inhibitory motor neurons and descending interneurons, 79% of strongly neuropeptide Y (NPY)-IR secretomotor neurons, 67% of weakly NPY-IR descending interneurons and motor neurons, and 46% of NK(1) receptor-IR neurons. In submucosal ganglia, NK(3) receptors were on 65% of calretinin-IR secretomotor/vasodilator neurons, 81% of NPY-IR cholinergic secretomotor neurons, 2% of vasoactive intestinal peptide-IR non-cholinergic secretomotor neurons and were completely absent from substance P-IR intrinsic primary afferent neurons. The results support physiological studies suggesting that NK(3) receptors mediate tachykinin transmission between myenteric sensory neurons and to interneurons and/or motor neurons in descending inhibitory and ascending excitatory pathways. |
[Mh] Termos MeSH primário: |
Íleo/metabolismo Cassinina/metabolismo Neurocinina A/metabolismo Neurônios/metabolismo Receptores da Neurocinina-3/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Carbocianinas/química Contagem de Células Feminino Corantes Fluorescentes/química Cobaias Técnicas In Vitro Cassinina/química Masculino Microscopia Confocal Plexo Mientérico/citologia Plexo Mientérico/metabolismo Neurocinina A/química Neurônios/citologia Piperidinas/farmacologia Receptores da Neurocinina-3/antagonistas & inibidores Plexo Submucoso/citologia Plexo Submucoso/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (Piperidines); 0 (Receptors, Neurokinin-3); 0 (SR 142801); 0 (cyanine dye 3); 136982-36-0 (3-(2-methoxybenzylamino)-2-phenylpiperidine); 63968-82-1 (Kassinin); 86933-74-6 (Neurokinin A) |
[Em] Mês de entrada: | 9911 |
[Cu] Atualização por classe: | 141120 |
[Lr] Data última revisão:
| 141120 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 990929 |
[St] Status: | MEDLINE |
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