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[PMID]:29025656
[Au] Autor:Xia B; Lv Y
[Ad] Endereço:Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, PR China; Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, Chongqing 400044, PR China.
[Ti] Título:Dual-delivery of VEGF and NGF by emulsion electrospun nanofibrous scaffold for peripheral nerve regeneration.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:253-264, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Controlled delivery of multiple therapeutic agents can be considered an effective approach in nerve injury due to its multifunction. In this study, recombinant human vascular endothelial growth factor (VEGF) and recombinant human nerve growth factor (NGF) were loaded on the surface and in the core of emulsion electrospun poly (l-lactic acid) (PLLA) nanofibrous scaffold, respectively. The in vitro studies showed that VEGF and NGF had a sequential release pattern in which most of the VEFG was released in the first few days but the NGF could be continuously released for >1month. The dual-delivery scaffold could enhance the neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) in vitro. Furthermore, this scaffold was applied to a critical sized defect in rat sciatic nerve model. Footprint analysis, electrophysiological tests, and histological analysis revealed that a significant improvement of neovascularization as well as nerve healing after 3months post-operation could be achieved by dual-delivery of VEGF and NGF. Taken together, the present study indicated that VEGF and NGF in emulsion electrospun nanofibrous scaffold had a synergistic effect on regeneration of vascularized nerve tissue.
[Mh] Termos MeSH primário: Emulsões/química
Nanofibras/química
Fator de Crescimento Neural/química
Nervo Isquiático/fisiologia
Tecidos Suporte/química
Fator A de Crescimento do Endotélio Vascular/química
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Feminino
Expressão Gênica/efeitos dos fármacos
Proteína Glial Fibrilar Ácida/genética
Proteína Glial Fibrilar Ácida/metabolismo
Células-Tronco Pluripotentes Induzidas/citologia
Células-Tronco Pluripotentes Induzidas/metabolismo
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Fator de Crescimento Neural/farmacologia
Regeneração Nervosa/efeitos dos fármacos
Células-Tronco Neurais/citologia
Células-Tronco Neurais/metabolismo
Poliésteres/química
Ratos
Ratos Sprague-Dawley
Recuperação de Função Fisiológica
Nervo Isquiático/patologia
Fator A de Crescimento do Endotélio Vascular/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Glial Fibrillary Acidic Protein); 0 (Microtubule-Associated Proteins); 0 (Polyesters); 0 (Vascular Endothelial Growth Factor A); 459TN2L5F5 (poly(lactide)); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:29283518
[Au] Autor:Ivanova VV; Milto IV; Sukhodolo IV; Serebryakova ON; Buzenkova AV
[Ti] Título:Digestive and Nondigestive Functions of Rodents' Salivary Glands.
[So] Source:Usp Fiziol Nauk;48(1):66-79, 2017 Jan-Mar.
[Is] ISSN:0301-1798
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Major salivary glands play a role not only in digestion, but also in regulation of other functions in rodents. In this review, we analyzed and summarized the data about the rodents' parotid, submandibular and sublingual salivary glands functions, which is not limited to the production of saliva and action of its hydrolytic enzymes on food in the oral cavity. In recent decades significantly expanded understanding of major salivary glands nondigestive functions. They are involved in excretion of metabolic products, maintaining fluid and electrolyte balance. Special attention has been paid to the characteristics of specific (parotin, sialorphin, etc.) and nonspecific (epidermal growth factor, nerve growth factor, kallikrein, etc.) active substances of the major salivary glands and their involvement in wound healing, mineral metabolism, regulation of hematopoiesis and immunity system. Summarized and analyzed major salivary glands endocrine function in the organs and systems. Available literature data suggest: the structure of the major salivary glands, as well as the synthesis and secretion of a number of biologically active substances are controlled by sex hormones. In turn, these biologically active factors of the salivary glands, as epidermal growth factor, and parotin, sialorphin, whose expression is regulated by androgens, have an impact on the morphological and functional state of the gonads. Thus, major salivary glands operate a wide range of functions and involved in the regulation of sexual behavior of reproductive function and maintaining homeostasis in the body.
[Mh] Termos MeSH primário: Glândula Parótida/fisiologia
Roedores/fisiologia
Proteínas e Peptídeos Salivares/metabolismo
Glândula Sublingual/fisiologia
Glândula Submandibular/fisiologia
[Mh] Termos MeSH secundário: Animais
Fator de Crescimento Epidérmico/genética
Fator de Crescimento Epidérmico/metabolismo
Regulação da Expressão Gênica
Hormônios Esteroides Gonadais/genética
Hormônios Esteroides Gonadais/metabolismo
Hematopoese/fisiologia
Imunidade Inata/efeitos dos fármacos
Calicreínas/genética
Calicreínas/metabolismo
Fator de Crescimento Neural/genética
Fator de Crescimento Neural/metabolismo
Saliva/química
Saliva/fisiologia
Proteínas e Peptídeos Salivares/genética
Proteínas e Peptídeos Salivares/farmacologia
Equilíbrio Hidroeletrolítico/fisiologia
Cicatrização/efeitos dos fármacos
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Gonadal Steroid Hormones); 0 (Salivary Proteins and Peptides); 62229-50-9 (Epidermal Growth Factor); 9061-61-4 (Nerve Growth Factor); EC 3.4.21.- (Kallikreins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE


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[PMID]:28964814
[Au] Autor:Gomita Y; Esumi S; Kitamura Y; Motoda H; Sendo T; Sagara H; Araki H; Mio M; Inoue S; Kano Y
[Ad] Endereço:Department of Pharmacy, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. Electronic address: pnh78qrk@okayama-u.ac.jp.
[Ti] Título:Intracranial self-stimulation and immobilization had different effects on neurite extension and the p38 MAPK pathway in PC12m3 cells.
[So] Source:Life Sci;190:78-83, 2017 Dec 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM: In mammals, rewarding and aversive states are motivational drivers of behavioral expression. However, it is unclear whether such states affect neuronal functions at the level of individual neurons. In the present study, the neuronal effects of rewarding and aversive states were investigated in using PC12 mutant cells (PC12m3 cells) with low sensitivity to nerve growth factor. MAIN METHODS: The intracranial self-stimulation (ICSS) and immobilization (IMM) methods were used to create rewarding and aversive states, respectively, in rats. Furthermore, experiments involving voluntary running on a wheel and forced running on a rotating rod were used to evaluate the effects of behavioral excitement on neurons. Then, the effects of plasma samples collected from the animals on neurite extension were examined microscopically, and p38 mitogen-activated protein kinase (MAPK) activity was assessed using Western blotting. KEY FINDINGS: Plasma samples from the ICSS and IMM rats facilitated neurite outgrowth to different degrees. However, their effects were not influenced by behavioral excitement. Furthermore, the plasma from the ICSS rats also induced upregulated p38 MAPK activity, whereas that from the IMM rats produced the same or slightly lower levels of MAPK activity to the control plasma. SIGNIFICANCE: These findings indicate that rewarding and aversive states might cause morphological changes, such as neurite extension. As for the effects of these states on p38 MAPK activity, the former state might directly increase p38 MAPK activity, but the latter state might have no effect or cause a slight reduction in p38 MAPK activity.
[Mh] Termos MeSH primário: Imobilização/psicologia
Neuritos/metabolismo
Recompensa
Autoestimulação
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Mh] Termos MeSH secundário: Animais
Aprendizagem da Esquiva/fisiologia
Comportamento Animal
Western Blotting
Masculino
Fator de Crescimento Neural/metabolismo
Células PC12
Ratos
Ratos Wistar
Corrida/fisiologia
Regulação para Cima
Proteínas Quinases p38 Ativadas por Mitógeno/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9061-61-4 (Nerve Growth Factor); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171002
[St] Status:MEDLINE


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[PMID]:28910445
[Au] Autor:Di G; Qi X; Zhao X; Zhang S; Danielson P; Zhou Q
[Ad] Endereço:Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
[Ti] Título:Corneal Epithelium-Derived Neurotrophic Factors Promote Nerve Regeneration.
[So] Source:Invest Ophthalmol Vis Sci;58(11):4695-4702, 2017 Sep 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To explore the neurotrophic factor expression in corneal epithelium and evaluate their effects on the trigeminal ganglion (TG) neurite outgrowth and corneal nerve regeneration in mice. Methods: The expression of neurotrophic factors was compared among the intact, regenerating, and regenerated mouse corneal epithelium. Mouse primary TG neurons were treated with the conditioned medium of mouse corneal epithelial cells. Nerve growth factor (NGF) neutralizing antibody and glial cell-derived neurotrophic factor (GDNF) neutralizing antibody were used to evaluate their roles in mouse corneal nerve regeneration and TG neurite outgrowth. The promoting effects of NGF and GDNF for the corneal nerve regeneration were further evaluated in the diabetic mice. Results: The expression of NGF and GDNF showed significant up-regulation in regenerating corneal epithelium and return to the preinjury levels in the regenerated epithelium, which was consistent with the progress of corneal subbasal nerve regeneration. The conditioned medium of corneal epithelial cells promoted the TG neurite outgrowth with extended branching and elongation. Furthermore, the blockage of either NGF or GDNF significantly impaired the promotion of the neurite outgrowth by the conditioned medium or the corneal nerve regeneration in normal mice. Moreover, the expression of NGF and GDNF was attenuated in the diabetic regenerating corneal epithelium as compared to that in normal mice, while exogenous NGF or GDNF supplement promoted the corneal epithelial and nerve regeneration in diabetic mice. Conclusions: Corneal epithelium expresses multiple neurotrophic factors, among which NGF and GDNF may play an important role in the corneal nerve regeneration.
[Mh] Termos MeSH primário: Córnea/inervação
Diabetes Mellitus Experimental/metabolismo
Epitélio Anterior/metabolismo
Fator Neurotrófico Derivado de Linhagem de Célula Glial/metabolismo
Fator de Crescimento Neural/metabolismo
Regeneração Nervosa/fisiologia
Nervo Trigêmeo/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Diabetes Mellitus Experimental/genética
Ensaio de Imunoadsorção Enzimática
Técnica Indireta de Fluorescência para Anticorpo
Fator Neurotrófico Derivado de Linhagem de Célula Glial/genética
Camundongos
Camundongos Endogâmicos C57BL
Fator de Crescimento Neural/genética
Neuritos/fisiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gdnf protein, mouse); 0 (Glial Cell Line-Derived Neurotrophic Factor); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21372


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[PMID]:28841320
[Au] Autor:Suh WS; Kim CS; Subedi L; Kim SY; Choi SU; Lee KR
[Ad] Endereço:Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
[Ti] Título:Iridoid Glycosides from the Twigs of Sambucus williamsii var. coreana and Their Biological Activities.
[So] Source:J Nat Prod;80(9):2502-2508, 2017 Sep 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Six new iridoid glycosides, sambucusides A-F (1-6), and two known derivatives (7 and 8) were isolated from a methanol extract of the twigs of Sambucus williamsii var. coreana. Their chemical structures were elucidated by spectroscopic methods, including NMR ( H and C NMR, H- H COSY, HMQC, HMBC, and NOESY) and HRMS. All isolated compounds (1-8) were evaluated for their antiproliferative activities against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and Bt549). Their effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells and their neuroprotective effects through induction of nerve growth factor (NGF) in C6 glioma cells were also examined. Compounds 2, 3, and 5 showed cytotoxic effects (IC 1.3-8.7 µM) against the SK-MEL-2 and Bt549 cell lines and inhibitory effects on NO production (IC of 0.9, 1.3, and 1.2 µM, respectively). Compounds 2, 4, and 8 exhibited NGF-releasing effects (147.0 ± 5.8%, 158.7 ± 5.2%, and 152.6 ± 7.3%, respectively).
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Glicosídeos Iridoides/isolamento & purificação
Glicosídeos Iridoides/farmacologia
Lipopolissacarídeos/farmacologia
Fator de Crescimento Neural/metabolismo
Fármacos Neuroprotetores/metabolismo
Óxido Nítrico/análise
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Linhagem Celular Tumoral
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Glicosídeos Iridoides/química
Lipopolissacarídeos/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Fator de Crescimento Neural/química
Fármacos Neuroprotetores/química
Sambucus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Iridoid Glycosides); 0 (Lipopolysaccharides); 0 (NGF protein, human); 0 (Neuroprotective Agents); 0 (sambucuside); 31C4KY9ESH (Nitric Oxide); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00410


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[PMID]:28827818
[Au] Autor:Youk J; Kim YS; Lim JA; Shin DY; Koh Y; Lee ST; Kim I
[Ad] Endereço:Division of Hematology/Oncology, Department of Internal Medicine, Seoul National University Hospital, Seoul, South Korea.
[Ti] Título:Depletion of nerve growth factor in chemotherapy-induced peripheral neuropathy associated with hematologic malignancies.
[So] Source:PLoS One;12(8):e0183491, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To investigate whether the depletion of nerve growth factor (NGF) is associated with the development of chemotherapy-induced peripheral neuropathy (CIPN) in patients with hematologic malignancy. METHODS: We prospectively enrolled hematologic cancer patients who had a plan to receive bortezomib, thalidomide, or vincristine. Baseline NGF levels were measured within one week before the start date of chemotherapy. Follow-up NGF levels were measured after four months from the start date of chemotherapy or the date when CIPN was initially diagnosed. RESULTS: Baseline and follow-up NGF pairs were measured in 45 patients (male/female = 27/18, median age = 63 years old). CIPN has developed in 28 patients. In the CIPN group, the level of NGF was significantly decreased after chemotherapy compared to the baseline (â–³NGF = -3.52 ±5.72; p-value = 0.003), while the NGF level of the no-CIPN group was not changed after chemotherapy. The differences in â–³NGF levels between the CIPN and no-CIPN group were more profound when analyzed in the subgroup of newly diagnosed multiple myeloma patients (â–³NGF = -4.14 ± 4.87 pg/ml for the CIPN group and +2.52 ± 8.39 pg/ml for the no-CIPN group; p-value = 0.043). CONCLUSIONS: This study shows that the depletion of NGF occurs during the development of CIPN, suggesting pathogenesis based on the role of NGF and therapeutic implications.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos
Neoplasias Hematológicas/tratamento farmacológico
Fator de Crescimento Neural/sangue
Doenças do Sistema Nervoso Periférico/induzido quimicamente
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Doenças do Sistema Nervoso Periférico/sangue
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183491


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[PMID]:28798232
[Au] Autor:Trabjerg E; Kartberg F; Christensen S; Rand KD
[Ad] Endereço:From the Department of Pharmacy, University of Copenhagen, 2100 Copenhagen E, Denmark and.
[Ti] Título:Conformational characterization of nerve growth factor-ß reveals that its regulatory pro-part domain stabilizes three loop regions in its mature part.
[So] Source:J Biol Chem;292(40):16665-16676, 2017 Oct 06.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nerve growth factor-ß (NGF) is essential for the correct development of the nervous system. NGF exists in both a mature form and a pro-form (proNGF). The two forms have opposing effects on neurons: NGF induces proliferation, whereas proNGF induces apoptosis via binding to a receptor complex of the common neurotrophin receptor (p75NTR) and sortilin. The overexpression of both proNGF and sortilin has been associated with several neurodegenerative diseases. Insights into the conformational differences between proNGF and NGF are central to a better understanding of the opposing mechanisms of action of NGF and proNGF on neurons. However, whereas the structure of NGF has been determined by X-ray crystallography, the structural details for proNGF remain elusive. Here, using a sensitive MS-based analytical method to measure the hydrogen/deuterium exchange of proteins in solution, we analyzed the conformational properties of proNGF and NGF. We detected the presence of a localized higher-order structure motif in the pro-part of proNGF. Furthermore, by comparing the hydrogen/deuterium exchange in the mature part of NGF and proNGF, we found that the presence of the pro-part in proNGF causes a structural stabilization of three loop regions in the mature part, possibly through a direct molecular interaction. Moreover, using tandem MS analyses, we identified two -linked and two -linked glycosylations in the pro-part of proNGF. These results advance our knowledge of the conformational properties of proNGF and NGF and help provide a rationale for the diverse biological effects of NGF and proNGF at the molecular level.
[Mh] Termos MeSH primário: Fator de Crescimento Neural/química
Precursores de Proteínas/química
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Medição da Troca de Deutério
Glicosilação
Seres Humanos
Fator de Crescimento Neural/genética
Domínios Proteicos
Precursores de Proteínas/genética
Estabilidade Proteica
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Precursors); 0 (pro-nerve growth factor, human); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.803320


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[PMID]:28716979
[Au] Autor:Bustamante A; López-Cancio E; Pich S; Penalba A; Giralt D; García-Berrocoso T; Ferrer-Costa C; Gasull T; Hernández-Pérez M; Millan M; Rubiera M; Cardona P; Cano L; Quesada H; Terceño M; Silva Y; Castellanos M; Garces M; Reverté S; Ustrell X; Marés R; Baiges JJ; Serena J; Rubio F; Salas E; Dávalos A; Montaner J
[Ad] Endereço:From the Neurovascular Research Laboratory, Vall d'Hebron Institut de Recerca (VHIR), Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Spain (A.B., A.P., D.G., T.G.-B., J.M.); Stroke Unit, Hospital Universitari Germans Trias i Pujol, Barcelona, Spain (E.L.-C., M.H.-P., M.M., A
[Ti] Título:Blood Biomarkers for the Early Diagnosis of Stroke: The Stroke-Chip Study.
[So] Source:Stroke;48(9):2419-2425, 2017 Sep.
[Is] ISSN:1524-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: Stroke diagnosis could be challenging in the acute phase. We aimed to develop a blood-based diagnostic tool to differentiate between real strokes and stroke mimics and between ischemic and hemorrhagic strokes in the hyperacute phase. METHODS: The Stroke-Chip was a prospective, observational, multicenter study, conducted at 6 Stroke Centers in Catalonia. Consecutive patients with suspected stroke were enrolled within the first 6 hours after symptom onset, and blood samples were drawn immediately after admission. A 21-biomarker panel selected among previous results and from the literature was measured by immunoassays. Outcomes were differentiation between real strokes and stroke mimics and between ischemic and hemorrhagic strokes. Predictive models were developed by combining biomarkers and clinical variables in logistic regression models. Accuracy was evaluated with receiver operating characteristic curves. RESULTS: From August 2012 to December 2013, 1308 patients were included (71.9% ischemic, 14.8% stroke mimics, and 13.3% hemorrhagic). For stroke versus stroke mimics comparison, no biomarker resulted included in the logistic regression model, but it was only integrated by clinical variables, with a predictive accuracy of 80.8%. For ischemic versus hemorrhagic strokes comparison, NT-proBNP (N-Terminal Pro-B-Type Natriuretic Peptide) >4.9 (odds ratio, 2.40; 95% confidence interval, 1.55-3.71; <0.0001) and endostatin >4.7 (odds ratio, 2.02; 95% confidence interval, 1.19-3.45; =0.010), together with age, sex, blood pressure, stroke severity, atrial fibrillation, and hypertension, were included in the model. Predictive accuracy was 80.6%. CONCLUSIONS: The studied biomarkers were not sufficient for an accurate differential diagnosis of stroke in the hyperacute setting. Additional discovery of new biomarkers and improvement on laboratory techniques seem necessary for achieving a molecular diagnosis of stroke.
[Mh] Termos MeSH primário: Isquemia Encefálica/sangue
Hemorragia Cerebral/sangue
Acidente Vascular Cerebral/sangue
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Amina Oxidase (contendo Cobre)/sangue
Apolipoproteína C-III/sangue
Biomarcadores/sangue
Isquemia Encefálica/diagnóstico
Estudos de Casos e Controles
Caspase 3/sangue
Moléculas de Adesão Celular/sangue
Hemorragia Cerebral/diagnóstico
Quimiocina CXCL1/sangue
Endostatinas/sangue
Proteína Ligante Fas/sangue
Feminino
Produtos de Degradação da Fibrina e do Fibrinogênio/metabolismo
Fibronectinas/sangue
Proteínas de Choque Térmico HSC70/sangue
Seres Humanos
Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina/sangue
Subunidade gama Comum de Receptores de Interleucina/sangue
Interleucina-17/sangue
Interleucina-6/sangue
Modelos Logísticos
Masculino
Metaloproteinase 9 da Matriz/sangue
Meia-Idade
Peptídeo Natriurético Encefálico/sangue
Fator de Crescimento Neural/sangue
Moléculas de Adesão de Célula Nervosa/sangue
Razão de Chances
Fragmentos de Peptídeos/sangue
Fosfopiruvato Hidratase/sangue
Estudos Prospectivos
Curva ROC
Receptores Tipo I de Fatores de Necrose Tumoral/sangue
Subunidade beta da Proteína Ligante de Cálcio S100/sangue
Acidente Vascular Cerebral/diagnóstico
Fator de von Willebrand/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Apolipoprotein C-III); 0 (Biomarkers); 0 (Cell Adhesion Molecules); 0 (Chemokine CXCL1); 0 (Endostatins); 0 (FASLG protein, human); 0 (Fas Ligand Protein); 0 (Fibrin Fibrinogen Degradation Products); 0 (Fibronectins); 0 (HSC70 Heat-Shock Proteins); 0 (HSPA8 protein, human); 0 (IGFBP3 protein, human); 0 (IL17A protein, human); 0 (IL2RG protein, human); 0 (IL6 protein, human); 0 (Insulin-Like Growth Factor Binding Protein 3); 0 (Interleukin Receptor Common gamma Subunit); 0 (Interleukin-17); 0 (Interleukin-6); 0 (NGF protein, human); 0 (Neural Cell Adhesion Molecules); 0 (Peptide Fragments); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (S100 Calcium Binding Protein beta Subunit); 0 (S100B protein, human); 0 (fibrin fragment D); 0 (pro-brain natriuretic peptide (1-76)); 0 (von Willebrand Factor); 114471-18-0 (Natriuretic Peptide, Brain); 9061-61-4 (Nerve Growth Factor); EC 1.4.3.21 (AOC3 protein, human); EC 1.4.3.21 (Amine Oxidase (Copper-Containing)); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (Caspase 3); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1161/STROKEAHA.117.017076


  9 / 6405 MEDLINE  
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[PMID]:28685530
[Au] Autor:Da Silva JT; Evangelista BG; Venega RAG; Oliveira ME; Chacur M
[Ad] Endereço:Department of Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, United States of America.
[Ti] Título:Early and late behavioral changes in sciatic nerve injury may be modulated by nerve growth factor and substance P in rats: a chronic constriction injury long-term evaluation.
[So] Source:J Biol Regul Homeost Agents;31(2):309-319, 2017 Apr-Jun.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Chronic constriction injury (CCI) simulates the symptoms of chronic nerve compression, which is characterized by allodynia and hyperalgesia. Nerve growth factor (NGF) is released after nerve injury by immune and Schwann cells and transported in retrograde fashion to the dorsal root ganglion (DRG), resulting in increased synthesis of Substance P (SP) and the triggering of neuropathic pain. Here we performed long-term evaluation of allodynia and hyperalgesia in a CCI model, and evaluated the effects of NGF and SP on the peripheral and central nervous systems. Most previous studies have shown deficits and molecular changes 14 days after surgery, however, the long-term effects have not been evaluated. We performed Randall-Selitto, Von Frey, Hargreaves and acetone tests for the entire 56 days post-surgery. Several of these deficits increased 14 to 56 days after CCI and we measured a constant increase in NGF levels in the DRG and spinal cord over the course of the experiment. In contrast, SP optical density maintained enhanced expression in DRG tissue from 14 to 56 days after CCI, whereas it was significantly increased only 56 days post-surgery in spinal cord. We perform long-term evaluation of symptoms associated with CCI and measure associated molecular changes. Moreover, by characterizing the behavioral signatures of this model, our work supports future studies.
[Mh] Termos MeSH primário: Hiperalgesia/metabolismo
Fator de Crescimento Neural/metabolismo
Nervo Isquiático/lesões
Nervo Isquiático/metabolismo
Substância P/metabolismo
[Mh] Termos MeSH secundário: Animais
Doença Crônica
Gânglios Espinais/metabolismo
Gânglios Espinais/patologia
Gânglios Espinais/fisiopatologia
Hiperalgesia/patologia
Hiperalgesia/fisiopatologia
Masculino
Ratos
Ratos Wistar
Nervo Isquiático/patologia
Nervo Isquiático/fisiopatologia
Medula Espinal/metabolismo
Medula Espinal/patologia
Medula Espinal/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
33507-63-0 (Substance P); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


  10 / 6405 MEDLINE  
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[PMID]:28677145
[Au] Autor:Takano S; Uchida K; Inoue G; Miyagi M; Aikawa J; Iwase D; Iwabuchi K; Matsumoto T; Satoh M; Mukai M; Minatani A; Takaso M
[Ad] Endereço:Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan.
[Ti] Título:Nerve growth factor regulation and production by macrophages in osteoarthritic synovium.
[So] Source:Clin Exp Immunol;190(2):235-243, 2017 Nov.
[Is] ISSN:1365-2249
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nerve growth factor (NGF) functions to modulate osteoarthritis (OA)-associated pain. Although recent studies suggest that tumour necrosis factor (TNF)-α and interleukin (IL)-1ß mediate NGF activity in human synovial fibroblasts, the regulation of NGF expression in human synovial macrophages remains unclear. Here, we examined the role of macrophages in the production and regulation of synovial (SYN) NGF in osteoarthritic knee joints by examining the mRNA expression of TNF-α and IL-1ß in freshly isolated CD14-positive (macrophage-rich fraction) and CD14-negative cells (fibroblast-rich fraction) in synovial tissue from OA patients by quantitative polymerase chain reaction. We also examined the effects of IL-1ß and TNF-α on NGF mRNA expression in cultured CD14-positive (macrophage-rich fraction) and CD14-negative cells (fibroblast-rich fraction). In addition, to examine the contribution of macrophages to NGF, TNF-α and IL-1ß expression, we injected clodronate liposomes systemically into STR/Ort mice, an osteoarthritis animal model, to deplete macrophages. TNF-α and IL-1ß mRNA levels in CD14-positive cells from the SYN of OA patients was significantly higher than that in CD14-negative cells, while NGF expression did not differ markedly between the two cell fractions. In addition, treatment of human cultured CD14-positive and -negative cells with IL-1ß and TNF-α enhanced NGF mRNA and protein levels. Expression of NGF, IL-1ß and TNF-α was also reduced significantly in STR/Ort mice upon macrophage depletion. These findings suggest that IL-1ß and TNF-α regulate NGF expression and production in synovial macrophages and fibroblasts in osteoarthritic joints.
[Mh] Termos MeSH primário: Macrófagos/metabolismo
Fator de Crescimento Neural/biossíntese
Fator de Crescimento Neural/genética
Osteoartrite do Joelho/metabolismo
Osteoartrite/metabolismo
Membrana Sinovial/imunologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Animais
Células Cultivadas
Ácido Clodrônico/administração & dosagem
Modelos Animais de Doenças
Feminino
Fibroblastos/efeitos dos fármacos
Regulação da Expressão Gênica
Seres Humanos
Interleucina-1beta/genética
Interleucina-1beta/metabolismo
Interleucina-1beta/farmacologia
Receptores de Lipopolissacarídeos/imunologia
Lipossomos
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Masculino
Camundongos
Fator de Crescimento Neural/imunologia
Osteoartrite/imunologia
Osteoartrite do Joelho/imunologia
Reação em Cadeia da Polimerase
Membrana Sinovial/metabolismo
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-1beta); 0 (Lipopolysaccharide Receptors); 0 (Liposomes); 0 (Tumor Necrosis Factor-alpha); 0813BZ6866 (Clodronic Acid); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE
[do] DOI:10.1111/cei.13007



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