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[PMID]: | 28188175 |
[Au] Autor: | Liu T; Wang Q; Li W; Mao F; Yue S; Liu S; Liu X; Xiao S; Xia L |
[Ad] Endereço: | State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and. |
[Ti] Título: | Gcn5 determines the fate of germline stem cells through degradation of Cyclin A. |
[So] Source: | FASEB J;31(5):2185-2194, 2017 May. | [Is] ISSN: | 1530-6860 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | The fluctuating CDK-CYCLIN complex plays a general role in cell-cycle control. Many types of stem cells use unique features of the cell cycle to facilitate asymmetric division. However, the manner in which these features are established remains poorly understood. The cell cycle of female germline stem cells (GSCs) is characterized by short G and very long G phases, making it an excellent model for the study of cell cycle control in stem cell fate determination. Using a female GSC model, we found Gcn5, the first discovered histone acetyltransferase, to maintain germline stem cells in ovaries. Results showed that Gcn5 is dispensable for the transcriptional silencing of , but interacts with Cyclin A to facilitate proper turnover in GSCs. Results also showed that Gcn5 promotes Cyclin A ubiquitination, which is dependent on its acetylating activity. Finally, results showed that knockdown of Cyclin A rescued the GSC-loss phenotype caused by lack of Gcn5. Collectively, these findings support the conclusion that Gcn5 acts through acetylation to facilitate Cyclin A ubiquitination and proper turnover, thereby determining the fate of GSCs.-Liu, T., Wang, Q., Li, W., Mao, F., Yue, S., Liu, S., Liu, X., Xiao, S., Xia, L. Gcn5 determines the fate of germline stem cells through degradation of Cyclin A. |
[Mh] Termos MeSH primário: |
Ciclo Celular/fisiologia Diferenciação Celular/genética Ciclina A/metabolismo Proteínas de Drosophila/metabolismo Células Germinativas/metabolismo Histona Acetiltransferases/metabolismo Células-Tronco/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Divisão Celular/fisiologia Drosophila melanogaster Feminino Ovário/metabolismo Fenótipo Transdução de Sinais Células-Tronco/citologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cyclin A); 0 (Drosophila Proteins); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (Pcaf protein, Drosophila) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171003 |
[Lr] Data última revisão:
| 171003 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170212 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1096/fj.201601217R |
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