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[PMID]:27939374
[Au] Autor:Vinci P; Bastone A; Schiarea S; Cappuzzello C; Del Prete A; Dander E; Biondi A; D'Amico G
[Ad] Endereço:Centro di Ricerca Tettamanti, Clinica Pediatrica, Università di Milano-Bicocca, Monza, Italy.
[Ti] Título:Mesenchymal stromal cell-secreted chemerin is a novel immunomodulatory molecule driving the migration of ChemR23-expressing cells.
[So] Source:Cytotherapy;19(2):200-210, 2017 Feb.
[Is] ISSN:1477-2566
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent cells characterized by broad immunomodulatory properties exploited for the treatment of inflammatory disorders. However, the efficacy of MSC-based therapy is highly variable and tightly linked to MSC culture conditions and treatment schedule. Thus, the identification of novel key molecules regulating MSC immunomodulatory activities in vivo might constitute a crucial step toward the optimization of currently available clinical protocols. In this regard, herein, we sought to determine whether the newly identified chemotactic protein, chemerin, plays a role in MSC-mediated regulation of inflammation. METHODS: Chemerin production by human MSCs was investigated under different culture conditions using enzyme-linked immunosorbent assay (ELISA). After purification, MSC-secreted chemerin was identified using mass spectrometry analysis and the biological activity of secreted isoforms was evaluated using migration assay. RESULTS: Bone marrow-derived MSCs secrete chemerin and express its receptors ChemR23 and CCRL2. Chemerin production is dependent on culture conditions and increases upon stimulation with inflammatory cytokines. In particular, platelet lysate (PL)-MSCs produce higher levels of chemerin compared with fetal bovine serum (FBS)-MSCs. Furthermore, chemerin is secreted by MSCs as an inactive precursor, which can be converted into its active form by exogenous chemerin-activating serine and cysteine proteases. DISCUSSION: Our data indicate that, in response to various inflammatory stimuli, MSCs secrete high amounts of inactive chemerin, which can then be activated by inflammation-induced tissue proteases. In light of these initial findings, we propose that further analysis of chemerin functions in vivo might constitute a crucial step toward optimizing MSC-based therapy for inflammatory diseases.
[Mh] Termos MeSH primário: Quimiotaxia/efeitos dos fármacos
Proteínas Quimerinas/farmacologia
Imunomodulação/efeitos dos fármacos
Células Mesenquimais Estromais/secreção
Receptores de Quimiocinas/metabolismo
[Mh] Termos MeSH secundário: Plaquetas/química
Técnicas de Cultura de Células
Extratos Celulares/química
Extratos Celulares/farmacologia
Células Cultivadas
Quimiotaxia/genética
Proteínas Quimerinas/genética
Proteínas Quimerinas/secreção
Meios de Cultura/metabolismo
Meios de Cultura/farmacologia
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Imunomodulação/genética
Inflamação/metabolismo
Inflamação/terapia
Transplante de Células-Tronco Mesenquimais/métodos
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Receptores de Quimiocinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CMKLR1 protein, human); 0 (Cell Extracts); 0 (Chimerin Proteins); 0 (Culture Media); 0 (Receptors, Chemokine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27222326
[Au] Autor:Gong X; Yin H; Shi Y; He X; Yu Y; Guan S; Kuai Z; Haji NM; Haji NM; Kong W; Shan Y
[Ad] Endereço:National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, Jilin Province, China.
[Ti] Título:Evaluation of the immunogenicity and protective effects of a trivalent chimeric norovirus P particle immunogen displaying influenza HA2 from subtypes H1, H3 and B.
[So] Source:Emerg Microbes Infect;5:e51, 2016 May 25.
[Is] ISSN:2222-1751
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ectodomain of the influenza A virus (IAV) hemagglutinin (HA) stem is highly conserved across strains and has shown promise as a universal influenza vaccine in a mouse model. In this study, potential B-cell epitopes were found through sequence alignment and epitope prediction in a stem fragment, HA2:90-105, which is highly conserved among virus subtypes H1, H3 and B. A norovirus (NoV) P particle platform was used to express the HA2:90-105 sequences from subtypes H1, H3 and B in loops 1, 2 and 3 of the protrusion (P) domain, respectively. Through mouse immunization and microneutralization assays, the immunogenicity and protective efficacy of the chimeric NoV P particle (trivalent HA2-PP) were tested against infection with three subtypes (H1N1, H3N2 and B) of IAV in Madin-Darby canine kidney cells. The protective efficacy of the trivalent HA2-PP was also evaluated preliminarily in vivo by virus challenge in the mouse model. The trivalent HA2-PP immunogen induced significant IgG antibody responses, which could be enhanced by a virus booster vaccination. Moreover, the trivalent HA2-PP immunogen also demonstrated in vitro neutralization of the H3 and B viruses, and in vivo protection against the H3 virus. Our results support the notion that a broadly protective vaccine approach using an HA2-based NoV P particle platform can provide cross-protection against challenge viruses of different IAV subtypes. The efficacy of the immunogen should be further enhanced for practicality, and a better understanding of the protective immune mechanism will be critical for the development of HA2-based multivalent vaccines.
[Mh] Termos MeSH primário: Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia
Imunogenicidade da Vacina
Vírus da Influenza A Subtipo H1N1/genética
Vírus da Influenza A Subtipo H3N2/genética
Vírus da Influenza B/genética
Vacinas contra Influenza/imunologia
Norovirus/genética
[Mh] Termos MeSH secundário: Células A549
Animais
Anticorpos Antivirais/sangue
Proteínas Quimerinas/administração & dosagem
Proteínas Quimerinas/genética
Proteínas Quimerinas/imunologia
Proteção Cruzada
Cães
Epitopos de Linfócito B/genética
Epitopos de Linfócito B/imunologia
Feminino
Seres Humanos
Imunização
Imunoglobulina G/sangue
Vírus da Influenza A Subtipo H1N1/imunologia
Vírus da Influenza A Subtipo H3N2/imunologia
Vírus da Influenza B/imunologia
Células Madin Darby de Rim Canino
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/prevenção & controle
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Chimerin Proteins); 0 (Epitopes, B-Lymphocyte); 0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Immunoglobulin G); 0 (Influenza Vaccines)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.1038/emi.2016.51


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[PMID]:26847448
[Au] Autor:Ahn KY; Lee MK; Kim DI; Park J; Min J; In Yang H; Lee J; Oh M; An J; Lee JW; Chu SH; Meyerhardt JA; Kim NK; Jeon JY
[Ad] Endereço:Cancer Prevention Center, Yonsei Cancer Canter, Department of Medicine, Yonsei Unversity College of Medicine, Yonsei University, Seoul, Republic of Korea.
[Ti] Título:Cardiopulmonary fitness, adiponectin, chemerin associated fasting insulin level in colorectal cancer patients.
[So] Source:Support Care Cancer;24(7):2927-35, 2016 07.
[Is] ISSN:1433-7339
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Since circulating level of insulin is associated with colorectal cancer prognosis, it is important to identify factors contributing to fasting insulin level in colorectal cancer patients. The purpose of the current study is to investigate the association of physical fitness, adiponectin, and chemerin levels with circulating level of insulin in colorectal cancer patients. METHODS: A total of 123 stage II-III colorectal cancer patients who completed standard cancer treatment were recruited. Anthropometric characteristics, fitness measurements, fasting insulin level, homeostasis model assessment of insulin resistance, lipid profiles, and adiponectin and chemerin levels were analyzed. RESULT: Cardiopulmonary fitness level inversely associated with fasting insulin levels (the least fit (1st tertile): 8.11 ± 0.64, moderately fit (2nd tertile): 6.02 ± 0.63, and highly fit (3rd tertile): 5.58 ± 0.66 µU/ml, unfit vs. moderately fit, p < 0.01; unfit vs. highly fit, p < 0.05) after adjustment for gender, age, stage, and BMI. In addition, fasting adiponectin and chemerin levels were associated with fasting insulin levels after adjustment for gender, age, stage, and BMI. In our combined analyses, participants with high adiponectin and low chemerin levels showed significantly lower fasting insulin levels (4.92 ± 0.75 vs. 8.07 ± 0.80 µU/ml, p < 0.01) compared with participants with low adiponectin and high chemerin levels. Multiple linear regression analysis confirmed that cardiopulmonary fitness and adiponectin levels (ß = -0.299, p = 0.002; ß = -0.201, p = 0.033) were independently associated with fasting insulin level. CONCLUSION: Our results suggest that physical fitness and adiponectin and chemerin levels may contribute to circulating levels of insulin. These results suggest that exercise may influence the prognosis of colorectal cancer patients by influencing physical fitness level, circulating levels of adiponectin and chemerin.
[Mh] Termos MeSH primário: Adiponectina/metabolismo
Proteínas Quimerinas/metabolismo
Exercício/fisiologia
Insulina/metabolismo
[Mh] Termos MeSH secundário: Neoplasias Colorretais
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adiponectin); 0 (Chimerin Proteins); 0 (Insulin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160206
[St] Status:MEDLINE
[do] DOI:10.1007/s00520-016-3095-4


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[PMID]:26315110
[Au] Autor:Alvi MA; McArt DG; Kelly P; Fuchs MA; Alderdice M; McCabe CM; Bingham V; McGready C; Tripathi S; Emmert-Streib F; Loughrey MB; McQuaid S; Maxwell P; Hamilton PW; Turkington R; James JA; Wilson RH; Salto-Tellez M
[Ad] Endereço:Northern Ireland Molecular Pathology Laboratory, Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, Northern Ireland, UK.
[Ti] Título:Comprehensive molecular pathology analysis of small bowel adenocarcinoma reveals novel targets with potential for clinical utility.
[So] Source:Oncotarget;6(25):20863-74, 2015 Aug 28.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Small bowel accounts for only 0.5% of cancer cases in the US but incidence rates have been rising at 2.4% per year over the past decade. One-third of these are adenocarcinomas but little is known about their molecular pathology and no molecular markers are available for clinical use. Using a retrospective 28 patient matched normal-tumor cohort, next-generation sequencing, gene expression arrays and CpG methylation arrays were used for molecular profiling. Next-generation sequencing identified novel mutations in IDH1, CDH1, KIT, FGFR2, FLT3, NPM1, PTEN, MET, AKT1, RET, NOTCH1 and ERBB4. Array data revealed 17% of CpGs and 5% of RNA transcripts assayed to be differentially methylated and expressed respectively (p < 0.01). Merging gene expression and DNA methylation data revealed CHN2 as consistently hypermethylated and downregulated in this disease (Spearman -0.71, p < 0.001). Mutations in TP53 which were found in more than half of the cohort (15/28) and Kazald1 hypomethylation were both were indicative of poor survival (p = 0.03, HR = 3.2 and p = 0.01, HR = 4.9 respectively). By integrating high-throughput mutational, gene expression and DNA methylation data, this study reveals for the first time the distinct molecular profile of small bowel adenocarcinoma and highlights potential clinically exploitable markers.
[Mh] Termos MeSH primário: Adenocarcinoma/patologia
Adenocarcinoma/terapia
Neoplasias Intestinais/patologia
Neoplasias Intestinais/terapia
Intestino Delgado/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais
Proteínas Quimerinas/genética
Ilhas de CpG
Metilação de DNA
Análise Mutacional de DNA
Epigênese Genética
Feminino
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Genes p53
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Meia-Idade
Mutação
Análise de Sequência com Séries de Oligonucleotídeos
Patologia Molecular
Estudos Retrospectivos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Chimerin Proteins); 0 (chimaerin-beta3 protein, human)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150829
[St] Status:MEDLINE


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[PMID]:25057852
[Au] Autor:Finalet Ferreiro J; Rouhigharabaei L; Urbankova H; van der Krogt JA; Michaux L; Shetty S; Krenacs L; Tousseyn T; De Paepe P; Uyttebroeck A; Verhoef G; Taghon T; Vandenberghe P; Cools J; Wlodarska I
[Ad] Endereço:Center for Human Genetics, KU Leuven, Leuven, Belgium.
[Ti] Título:Integrative genomic and transcriptomic analysis identified candidate genes implicated in the pathogenesis of hepatosplenic T-cell lymphoma.
[So] Source:PLoS One;9(7):e102977, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatosplenic T-cell lymphoma (HSTL) is an aggressive lymphoma cytogenetically characterized by isochromosome 7q [i(7)(q10)], of which the molecular consequences remain unknown. We report here results of an integrative genomic and transcriptomic (expression microarray and RNA-sequencing) study of six i(7)(q10)-positive HSTL cases, including HSTL-derived cell line (DERL-2), and three cases with ring 7 [r(7)], the recently identified rare variant aberration. Using high resolution array CGH, we profiled all cases and mapped the common deleted region (CDR) at 7p22.1p14.1 (34.88 Mb; 3506316-38406226 bp) and the common gained region (CGR) at 7q22.11q31.1 (38.77 Mb; 86259620-124892276 bp). Interestingly, CDR spans a smaller region of 13 Mb (86259620-99271246 bp) constantly amplified in cases with r(7). In addition, we found that TCRG (7p14.1) and TCRB (7q32) are involved in formation of r(7), which seems to be a byproduct of illegitimate somatic rearrangement of both loci. Further transcriptomic analysis has not identified any CDR-related candidate tumor suppressor gene. Instead, loss of 7p22.1p14.1 correlated with an enhanced expression of CHN2 (7p14.1) and the encoded ß2-chimerin. Gain and amplification of 7q22.11q31.1 are associated with an increased expression of several genes postulated to be implicated in cancer, including RUNDC3B, PPP1R9A and ABCB1, a known multidrug resistance gene. RNA-sequencing did not identify any disease-defining mutation or gene fusion. Thus, chromosome 7 imbalances remain the only driver events detected in this tumor. We hypothesize that the Δ7p22.1p14.1-associated enhanced expression of CHN2/ß2-chimerin leads to downmodulation of the NFAT pathway and a proliferative response, while upregulation of the CGR-related genes provides growth advantage for neoplastic δγT-cells and underlies their intrinsic chemoresistance. Finally, our study confirms the previously described gene expression profile of HSTL and identifies a set of 24 genes, including three located on chromosome 7 (CHN2, ABCB1 and PPP1R9A), distinguishing HSTL from other malignancies.
[Mh] Termos MeSH primário: Proteínas Quimerinas/genética
Aberrações Cromossômicas
Cromossomos Humanos Par 7
Neoplasias Hepáticas/genética
Linfoma de Células T/genética
Proteínas dos Microfilamentos/genética
Proteínas de Neoplasias/genética
Proteínas do Tecido Nervoso/genética
Neoplasias Esplênicas/genética
[Mh] Termos MeSH secundário: Subfamília B de Transportador de Cassetes de Ligação de ATP/genética
Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo
Adolescente
Adulto
Criança
Proteínas Quimerinas/metabolismo
Mapeamento Cromossômico
Feminino
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Loci Gênicos
Genoma Humano
Seres Humanos
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/patologia
Linfoma de Células T/metabolismo
Linfoma de Células T/patologia
Masculino
Proteínas dos Microfilamentos/metabolismo
Meia-Idade
Proteínas de Neoplasias/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Neoplasias Esplênicas/metabolismo
Neoplasias Esplênicas/patologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ABCB1 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (Chimerin Proteins); 0 (Microfilament Proteins); 0 (Neoplasm Proteins); 0 (Nerve Tissue Proteins); 0 (beta-chimaerin); 0 (chimaerin-beta3 protein, human); 0 (neurabin)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140725
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0102977


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[PMID]:24854763
[Au] Autor:Chen M; Lin WR; Lu CH; Chen CC; Huang YC; Liao WL; Tsai FJ
[Ad] Endereço:Department of Urology, Mackay Memorial Hospital, Taipei, Taiwan; Mackay Medicine, Nursing and Management College, Taipei, Taiwan; School of Medicine, Mackay Medical College, New Taipei City, Taiwan.
[Ti] Título:Chimerin 2 genetic polymorphisms are associated with non-proliferative diabetic retinopathy in Taiwanese type 2 diabetic patients.
[So] Source:J Diabetes Complications;28(4):460-3, 2014 Jul-Aug.
[Is] ISSN:1873-460X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: To investigate whether chimerin 2 (CHN2) genetic polymorphisms were associated with the susceptibility to diabetic retinopathy (DR) in Taiwanese individuals with type 2 diabetes. METHODS: This case-control study comprised of 171 individuals with DR and 548 without DR. Four rs39059, rs2023908, rs1002630 and rs1362363 polymorphism of CHN2 were genotyped for each subjects. All subjects underwent a complete ophthalmologic examination, and basic information (age, gender, age at diagnosis of diabetes, and ocular history of the patient) was record. Several clinical parameters (systolic and diastolic blood pressure, waist and hip circumferences, body mass index levels, fasting glucose and HbA1c) were measured. RESULTS: Logistic regressions were used to analyze odds ratios between SNPs and DR after controlling for gender, systolic blood pressure, waist and hip ratio, duration of diabetes, serum HbA1c levels and nephropathy classification. A protective effect of rs1002630 (GA+AA) and rs1362363 (AG+GG) [odds ratio (OR) (95% confidence interval)=0.45 (0.22-0.88), 0.66 (0.44-0.99), respectively) was observed. Furthermore, the protective effect of rs1002630 was observed when compared subjects with non-proliferative DR with subjects without DR [OR=0.25 (95%C.I. = 0.09-0.73)]. CONCLUSIONS: This study showed that the rs1002630 of CHN2 were associated with DR risk and non-proliferative DR risk in Taiwanese individuals with type 2 diabetes. Variations at this locus may contribute to the pathogenesis of DR.
[Mh] Termos MeSH primário: Proteínas Quimerinas/genética
Diabetes Mellitus Tipo 2/complicações
Retinopatia Diabética/genética
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Idoso
Alelos
Grupo com Ancestrais do Continente Asiático
Estudos de Casos e Controles
Proteínas Quimerinas/metabolismo
China/etnologia
Estudos Transversais
Retinopatia Diabética/metabolismo
Retinopatia Diabética/patologia
Retinopatia Diabética/fisiopatologia
Feminino
Estudos de Associação Genética
Predisposição Genética para Doença
Seres Humanos
Modelos Logísticos
Masculino
Meia-Idade
Retina/patologia
Índice de Gravidade de Doença
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chimerin Proteins); 0 (chimaerin-beta3 protein, human)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:140624
[Lr] Data última revisão:
140624
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140524
[St] Status:MEDLINE


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[PMID]:24702860
[Au] Autor:Fülöp P; Seres I; Lorincz H; Harangi M; Somodi S; Paragh G
[Ad] Endereço:Division of Metabolic Diseases, Department of Internal Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
[Ti] Título:Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients.
[So] Source:J Cell Mol Med;18(7):1313-20, 2014 Jul.
[Is] ISSN:1582-4934
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The prevalence of obesity has been increasing worldwide. Chemerin is a recently discovered adipokine secreted by the enlarged adipose tissue with diverse biological effects that are not well detailed yet. This study aimed to elucidate the potential role of chemerin in oxidative stress and inflammation that are characteristics for excess weight and may eventually lead to insulin resistance and atherosclerotic complications. We also analysed the associations between chemerin and classical adipokines, namely leptin and adiponectin. Therefore, we investigated non-diabetic obese patients without manifest cardiovascular disease and compared their data to healthy lean individuals. Chemerin correlated positively with markers of oxidative stress and inflammation, while it showed a negative correlation with the measure of antioxidant status, characterized by the HDL-linked paraoxonase-1 enzyme. Chemerin also correlated positively with leptin and negatively with adiponectin respectively. In our study population, oxidized low-density lipoprotein and high-sensitivity C-reactive protein were found to be the strongest predictors of chemerin level. We conclude that chemerin may contribute to chronic inflammation and increased oxidative stress in obese individuals, even in the absence of manifest insulin resistance.
[Mh] Termos MeSH primário: Adipocinas/metabolismo
Biomarcadores/metabolismo
Proteínas Quimerinas/metabolismo
Inflamação/patologia
Obesidade/patologia
Estresse Oxidativo
[Mh] Termos MeSH secundário: Adiponectina/metabolismo
Tecido Adiposo/metabolismo
Tecido Adiposo/patologia
Arildialquilfosfatase/metabolismo
Estudos de Casos e Controles
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Inflamação/complicações
Resistência à Insulina
Leptina/metabolismo
Lipoproteínas LDL/metabolismo
Obesidade/etiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adipokines); 0 (Adiponectin); 0 (Biomarkers); 0 (Chimerin Proteins); 0 (Leptin); 0 (Lipoproteins, LDL); 0 (oxidized low density lipoprotein); EC 3.1.8.1 (Aryldialkylphosphatase)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140408
[St] Status:MEDLINE
[do] DOI:10.1111/jcmm.12282


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[PMID]:24506805
[Au] Autor:Li L; Huang C; Zhang X; Wang J; Ma P; Liu Y; Xiao T; Zabel BA; Zhang JV
[Ad] Endereço:Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
[Ti] Título:Chemerin-derived peptide C-20 suppressed gonadal steroidogenesis.
[So] Source:Am J Reprod Immunol;71(3):265-77, 2014 Mar.
[Is] ISSN:1600-0897
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:PROBLEM: Chemerin is a novel chemo-attractant and adipokine involved in leukocyte recruitment, inflammation, adipogenesis, lipid/carbohydrate metabolism, and reproduction. Based on the bioinformatic search for putative small peptides in the conserved region of pre-pro-chemerin, an evolutionary conserved region flanked by potential convertase cleavage sites was identified and we named it as C-20. The binding capacity of C-20 to chemerin receptors and its potential bioactivities were investigated in this study. METHOD OF STUDY: Radioligand binding assay, receptor internalization assay, and early response gene C-FOS simulation, cAMP assay were carried out in chemokine-like receptor 1 (CMKLR1)/HEK293 transfectants and G protein-coupled receptor 1 (GPR1)/HEK293 transfectants. In vitro transwell chemotaxis assay in CMKLR1/L1.2 transfectants, primary Leydig cell culture, and antral follicle culture was explored to investigate the bioactivity of C-20. RESULTS: C-20 bound to chemerin receptors CMKLR1 and GPR1 with high affinity triggered CMKLR1 internalization and stimulated subsequent signal C-FOS expression and cAMP production. C-20, such as chemerin, showed CMKLR1-dependent chemotactic property. Furthermore, in primary Leydig cells and antral follicles, C-20 showed similar but less potent suppressive effect on human chorionic gonadotropin-stimulated testosterone production and progesterone production, compared with chemerin. CONCLUSION: The novel chemerin-derived C-20 peptide binds to chemerin receptors CMKLR1 and GPR1 and showed similar but less potent bioactivity in chemotaxis and the suppression of gonadal steroidogenesis, suggesting that after optimization, C-20 is possible to be a useful experimental tool for the understanding of the biological functions of chemerin/CMKLR1 and chemerin/GPR1 signaling.
[Mh] Termos MeSH primário: Proteínas Quimerinas/metabolismo
Fragmentos de Peptídeos/metabolismo
Progesterona/biossíntese
Testículo/fisiologia
Testosterona/biossíntese
[Mh] Termos MeSH secundário: Quimiotaxia
Proteínas Quimerinas/genética
Biologia Computacional
AMP Cíclico/metabolismo
Células HEK293
Seres Humanos
Células Intersticiais do Testículo
Masculino
Fragmentos de Peptídeos/genética
Ligação Proteica
Proteínas Proto-Oncogênicas c-fos/genética
Proteínas Proto-Oncogênicas c-fos/metabolismo
Receptores de Quimiocinas/genética
Receptores de Quimiocinas/metabolismo
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CMKLR1 protein, human); 0 (Chimerin Proteins); 0 (GPR1 protein, human); 0 (Peptide Fragments); 0 (Proto-Oncogene Proteins c-fos); 0 (Receptors, Chemokine); 0 (Receptors, G-Protein-Coupled); 0 (chemerin-derived peptide C-20); 3XMK78S47O (Testosterone); 4G7DS2Q64Y (Progesterone); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140211
[St] Status:MEDLINE
[do] DOI:10.1111/aji.12164


  9 / 51 MEDLINE  
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[PMID]:24430297
[Au] Autor:Zubeldia-Brenner L; Gutierrez-Uzquiza A; Barrio-Real L; Wang H; Kazanietz MG; Leskow FC
[Ad] Endereço:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, IQUIBICEN-CONICET, Universidad de Buenos Aires, C1428EGA, Buenos Aires, Argentina.
[Ti] Título:ß3-chimaerin, a novel member of the chimaerin Rac-GAP family.
[So] Source:Mol Biol Rep;41(4):2067-76, 2014.
[Is] ISSN:1573-4978
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (ß1- and ß2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that ß1- and ß2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named ß3-chimaerin. Expression profile analysis revealed predominantly low levels for the ß3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, ß3-chimaerin has a unique N-terminal domain. Studies in cells established that ß3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of ß3-chimaerin for phorbol ester-induced translocation relative to ß2-chimaerin suggests differential ligand accessibility to the C1 domain.
[Mh] Termos MeSH primário: Proteínas Quimerinas/genética
Proteínas Quimerinas/metabolismo
Isoformas de Proteínas
[Mh] Termos MeSH secundário: Processamento Alternativo
Sequência de Aminoácidos
Animais
Sequência de Bases
Células COS
Linhagem Celular
Cercopithecus aethiops
Proteínas Quimerinas/química
Ativação Enzimática
Expressão Gênica
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/efeitos dos fármacos
Ordem dos Genes
Seres Humanos
Dados de Sequência Molecular
Especificidade de Órgãos/genética
Regiões Promotoras Genéticas
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Acetato de Tetradecanoilforbol/farmacologia
Proteínas rac de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chimerin Proteins); 0 (Protein Isoforms); 0 (chimaerin-beta3 protein, human); EC 3.6.5.2 (rac GTP-Binding Proteins); NI40JAQ945 (Tetradecanoylphorbol Acetate)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140117
[St] Status:MEDLINE
[do] DOI:10.1007/s11033-014-3055-3


  10 / 51 MEDLINE  
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[PMID]:24389278
[Au] Autor:Bagheri S; Mousavi Gargari SL; Rasooli I; Nazarian S; Alerasol M
[Ad] Endereço:Department of Biology, Shahed University, Tehran, Iran.
[Ti] Título:A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli.
[So] Source:Braz J Infect Dis;18(3):308-14, 2014 May-Jun.
[Is] ISSN:1678-4391
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells. METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated. RESULTS: Antibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells. DISCUSSION: The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.
[Mh] Termos MeSH primário: Antígenos de Bactérias/genética
Proteínas Quimerinas/imunologia
Escherichia coli Enterotoxigênica/genética
Infecções por Escherichia coli/microbiologia
Proteínas de Escherichia coli/genética
[Mh] Termos MeSH secundário: Animais
Western Blotting
Proteínas Quimerinas/química
Escherichia coli Enterotoxigênica/imunologia
Infecções por Escherichia coli/imunologia
Feminino
Camundongos
Camundongos Endogâmicos BALB C
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (CS6 antigen, E coli); 0 (Chimerin Proteins); 0 (Escherichia coli Proteins)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140519
[Lr] Data última revisão:
140519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140107
[St] Status:MEDLINE



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