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[PMID]:28647372
[Au] Autor:Zhao P; Kuai J; Gao J; Sun L; Wang Y; Yao L
[Ad] Endereço:Department of Anesthesiology, Third Hospital of Xi'an, 10 Fengcheng Three Road, Xi'an, Shanxi 710018, China.
[Ti] Título:Delta opioid receptor agonist attenuates lipopolysaccharide-induced myocardial injury by regulating autophagy.
[So] Source:Biochem Biophys Res Commun;492(1):140-146, 2017 Oct 07.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Previous studies have described the protective effects of DADLE on myocardial injury in sepsis. Recently, autophagy has been shown to be an innate defense mechanism in sepsis-related myocardial injury. However, whether DADLE has an pro-autophagic effect is yet to be elucidated. The present study aimed to investigate the effect of DADLE on the regulation of autophagy during sepsis. METHODS: Male mice were subjected to LPS or vehicle intraperitoneal injection. After LPS injection, mice received either DADLE, Naltrindole or vehicle. ELISA and JC-1 were used to evaluate the level cTnI and Mitochondrial membrane potential. Cardiac ultrastructural and autophagosomes were visualized by transmission electron microscopy. The relative protein levels were analyzed by Western blot. RESULTS: The results showed that treatment with DADLE both immediately or 4 h after LPS intraperitoneal injection could improve the survival rate of mice with endotoxemic. DADLE could ease myocardium ultrastructure injury induced by LPS, this cardioprotective effect was also seen in increased MMP levels, and decreased cTnI levels. Through observation of transmission electron microscopy and Western blot we have discovered that the amount of autophagosome and the expression of autophagy related protein LC3II, Beclin1 were significantly increased with DADLE treatment. DADLE promoted LPS-induced autophagosome maturation as indicated by the increased LAMP-1 protein level and decreased SQSTM1/p62 protein level. The selective δ-opioid receptor antagonist Naltrindole play an opposite effects. CONCLUSIONS: DADLE could improve the survival and protect myocardial dysfunction in mice with LPS-induced endotoxemia. This effect was related to the increase of autophagy.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Leucina Encefalina-2-Alanina/farmacologia
Lipopolissacarídeos/farmacologia
Isquemia Miocárdica/tratamento farmacológico
Receptores Opioides delta/agonistas
[Mh] Termos MeSH secundário: Animais
Injeções Intraperitoneais
Lipopolissacarídeos/administração & dosagem
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Eletrônica de Transmissão
Isquemia Miocárdica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Receptors, Opioid, delta); 63631-40-3 (Enkephalin, Leucine-2-Alanine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE


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[PMID]:28173925
[Au] Autor:Abbood A; Smadja C; Taverna M; Herrenknecht C
[Ad] Endereço:Institut Galien Paris-Sud, UMR CNRS 8612, Proteins and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France; Faculty of Pharmacy, Tishreen University, Boulevard Aleppo, Latakia, Syria.
[Ti] Título:Hydrophilic interaction liquid chromatography for dalargin separation from its structural analogues and side products.
[So] Source:J Chromatogr A;1498:155-162, 2017 May 19.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Retention behaviour of Dalargin and five peptide analogues of Leu-enkephalin, has been extensively studied by hydrophilic interaction liquid chromatography (HILIC) on a bare silica stationary phase (Atlantis HILIC silica). The influence of buffer pH, ionic strength, and organic modifier content on peptide retentions was examined. Variation of organic modifier content (70-90% ACN) shows that, as expected, the most polar peptide, Dalargin, is the most retained. Moreover, at acidic pH, the retention mechanism for all the peptides studied seems to rely, mainly, on adsorption phenomenon. By varying the pswH buffer (between 4.4-7.5), we observed that the retention of all the peptides was mainly governed by their total number of charges, whatever the variation (increase or decrease) of their retention factor. At pswH 7.5, an increase of the cationic counter-ion concentration (NH ) lead to a decrease of the retention factor of Dalargin, suggesting a weak cation exchange for this peptide. For the other peptides, the variation of the retention factors was negligible between 5-15mM. Above 15mM, the retention factors of all the peptides increased, probably due to an increase of the water layer thickness at the surface of the stationary phase. In the second part of the study, qualitative analysis of non-purified dalargin, resulting from solid-phase synthesis, was realized. Optimisation of the separation of the target peptide from its side products has been first performed with UV detection. Then, by coupling the HILIC column with ESI-MS, using the optimal separation conditions, it was possible to identify Dalargin and to propose the amino-acids sequence of its side-products.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Leucina Encefalina-2-Alanina/análogos & derivados
Encefalina Leucina/análise
[Mh] Termos MeSH secundário: Compostos de Amônio/química
Encefalina Leucina/química
Encefalina Leucina/isolamento & purificação
Leucina Encefalina-2-Alanina/análise
Leucina Encefalina-2-Alanina/química
Leucina Encefalina-2-Alanina/isolamento & purificação
Concentração de Íons de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Íons/química
Concentração Osmolar
Peptídeos/análise
Peptídeos/isolamento & purificação
Dióxido de Silício/química
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Ions); 0 (Peptides); 58822-25-6 (Enkephalin, Leucine); 63631-40-3 (Enkephalin, Leucine-2-Alanine); 7631-86-9 (Silicon Dioxide); V13505565P (enkephalin-Leu, Ala(2)-Arg(6)-)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE


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[PMID]:27627741
[Au] Autor:Merkenschlager A; Bloßfeld M; Nieber K
[Ad] Endereço:Klinik und Poliklinik für Kinder und Jugendliche der Universität Leipzig, Abteilung Neuropädiatrie, Leipzig, Germany.
[Ti] Título:The Delta-Opioid Receptor Agonist D-Ala2-D-Leu2-Enkephalin Enhances Hypoxic Depression of Excitatory Synaptic Transmission and Improves Recovery of Rat Cortical Neurons from Hypoxia in vitro.
[So] Source:Pharmacology;99(1-2):9-18, 2017.
[Is] ISSN:1423-0313
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:We investigated the influence of the delta-opioid receptor-preferring agonist D-Ala2-D-Leu2-enkephalin (DADLE) in vitro during long- and short-term hypoxia on the single cortical neuron membrane currents, the postsynaptic currents (PSCs), and the postsynaptic potentials (PSPs) in rats. Rat cortical pyramidal neurons showed 2 distinct and prognostically relevant responses to hypoxia. Type A neurons that responded to hypoxia by an inward current, followed by a steady outward current, were shown to recover during subsequent reoxygenation. In contrast, type B neurons that responded by a steady inward current, indicative of gradual anoxic depolarisation, suffered irreversible membrane dysfunction and did not recover completely during reoxygenation. Pre-treatment with 1 µmol/l DADLE attenuated the hypoxic inward current and favored complete recovery of holding current and input resistance during reoxygenation, even when neurons were challenged by a second exposure to hypoxia. DADLE enhanced the inhibitory effect of hypoxia on PSPs and PSCs. We assume that this neuroprotective effect is transmitted by the additive effects of DADLE on the hypoxic PSP/PSC suppression, thereby inhibiting presynaptic glutamate release.
[Mh] Termos MeSH primário: Córtex Cerebral/efeitos dos fármacos
Leucina Encefalina-2-Alanina/farmacologia
Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Receptores Opioides delta/agonistas
Transmissão Sináptica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Analgésicos Opioides/farmacologia
Animais
Hipóxia Celular/efeitos dos fármacos
Hipóxia Celular/fisiologia
Córtex Cerebral/citologia
Córtex Cerebral/fisiologia
Potenciais Pós-Sinápticos Excitadores/fisiologia
Feminino
Masculino
Neurônios/fisiologia
Técnicas de Cultura de Órgãos
Ratos
Ratos Wistar
Receptores Opioides delta/fisiologia
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics, Opioid); 0 (Receptors, Opioid, delta); 63631-40-3 (Enkephalin, Leucine-2-Alanine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE
[do] DOI:10.1159/000449468


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[PMID]:27909965
[Au] Autor:Pinaeva OG; Sazonova EN; Lebed'ko OA; Timoshin SS
[Ad] Endereço:Far Eastern State Medical University, Ministry of Health of Russian Federation, Khabarovsk, Russia. pinaeva_og@mail.ru.
[Ti] Título:Correction of Negative Effect of Antenatal Hypoxia on Liver Tissue Homeostasis in Newborn Albino Rats with Opioid Peptides.
[So] Source:Bull Exp Biol Med;162(2):203-206, 2016 Dec.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We studied the possibility of correction of the negative effects of antenatal hypoxia on the liver tissue homeostasis in 7-day-old albino rats by administration of opioid peptides in a dose of 100 µg/kg on postnatal days 2-6. Administration of mixed µ/δ-opioid receptor agonist Dalargin neutralized deviations of gravimetric indicators, parameters of proliferative activity, and activity of the nucleolar apparatus of hepatocytes. Administration of the non-opiate Leu-enkephalin analogue did not normalize gravimetric parameters and nucleolar apparatus parameters, however, it significantly increased the pool of proliferating hepatocytes. Both peptides significantly reduced the intensity of free radical oxidation, improved antioxidant antiradical defense and resistance to peroxidation in the liver tissue of animals subjected to antenatal hypoxia.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Leucina Encefalina-2-Alanina/análogos & derivados
Hepatócitos/efeitos dos fármacos
Hipóxia/tratamento farmacológico
Fígado/efeitos dos fármacos
Peptídeos Opioides/farmacologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Peso Corporal/efeitos dos fármacos
Núcleo Celular/efeitos dos fármacos
Núcleo Celular/metabolismo
Núcleo Celular/ultraestrutura
Leucina Encefalina-2-Alanina/farmacologia
Feminino
Radicais Livres/antagonistas & inibidores
Hepatócitos/metabolismo
Hepatócitos/patologia
Homeostase/efeitos dos fármacos
Hipóxia/metabolismo
Hipóxia/patologia
Fígado/metabolismo
Fígado/patologia
Tamanho do Órgão/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Free Radicals); 0 (Opioid Peptides); 63631-40-3 (Enkephalin, Leucine-2-Alanine); V13505565P (enkephalin-Leu, Ala(2)-Arg(6)-)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170210
[Lr] Data última revisão:
170210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE


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[PMID]:27016387
[Au] Autor:Wang SY; Duan YL; Zhao B; Wang XR; Zhao Z; Zhang GM
[Ad] Endereço:Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China.
[Ti] Título:Effect of delta opioid receptor activation on spatial cognition and neurogenesis in cerebral ischemic rats.
[So] Source:Neurosci Lett;620:20-6, 2016 May 04.
[Is] ISSN:1872-7972
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:This study aimed to investigate whether a selective delta opioid receptor agonist, [D-Ala2, D-Leu5]-Enkephalin (DADLE), regulates neurogenesis in the hippocampus of ischemic rats. Using an intracerebral cannula, rats were subjected to cerebral ischemia using the standard four-vessel occlusion. DADLE (2.5nmol), DADLE (2.5nmol) with naltrindole (NAL) (2.5nmol), or vehicle was administered at the onset of reperfusion. Bromodeoxyuridine (BrdU, 100mg/kg, intraperitoneal) was used to label newly formed cells from days 1 to 7 after ischemia. Immunohistochemistry was used to evaluate cell proliferation and apoptosis and differentiation 7days 28 days, respectively, after ischemia. Morris water maze test was conducted to test spatial learning and memory 23-27 days after ischemia. We found that DADLE treatment improved performance in the Morris water maze test, promoted proliferation and differentiation of newly formed neurons, and inhibited differentiation into astrocytes in a rat model of cerebral ischemia. Furthermore, the protective effects of DADLE were significantly reversed by co-administration of NAL (P<0.05), a highly potent and selective delta opioid receptor antagonist. Our findings suggest that DADLE promotes spatial cognitive function recovery and regulates neurogenesis after ischemia, which may provide a promising therapeutic strategy for cerebral ischemia.
[Mh] Termos MeSH primário: Isquemia Encefálica/tratamento farmacológico
Leucina Encefalina-2-Alanina/uso terapêutico
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores/uso terapêutico
Receptores Opioides delta/agonistas
Aprendizagem Espacial/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose
Encéfalo/efeitos dos fármacos
Encéfalo/patologia
Isquemia Encefálica/patologia
Isquemia Encefálica/psicologia
Diferenciação Celular
Proliferação Celular
Leucina Encefalina-2-Alanina/farmacologia
Masculino
Aprendizagem em Labirinto/efeitos dos fármacos
Neurogênese
Neurônios/patologia
Fármacos Neuroprotetores/farmacologia
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Receptors, Opioid, delta); 63631-40-3 (Enkephalin, Leucine-2-Alanine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160327
[St] Status:MEDLINE


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[PMID]:25592480
[Au] Autor:Liu H; Chen B; Li S; Yao J
[Ad] Endereço:Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:Dose-dependent neuroprotection of delta-opioid peptide [D-Ala(2), D-Leu(5)] enkephalin on spinal cord ischemia-reperfusion injury by regional perfusion into the abdominal aorta in rabbits.
[So] Source:J Vasc Surg;63(4):1074-81, 2016 Apr.
[Is] ISSN:1097-6809
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: In our prior study, we showed that delta-opioid peptide [D-Ala(2), D-Leu(5)] enkephalin (DADLE), by regional perfusion into the abdominal aorta, could protect the spinal cord against ischemia-reperfusion (I/R) injury caused by aortic occlusion. However, the relative dose-response effects of DADLE still remain unclear. This study investigated whether DADLE has a dose-dependent efficiency on spinal cord I/R injury. METHODS: New Zealand White rabbits were randomly divided into one of six groups: normal saline (NS; n = 8), DADLE (D) groups D0.0005 (n = 8), D0.005 (n = 8), D0.05 (n = 8), and D0.5 mg/kg (n = 8), and a sham group (n = 6). In the NS and DADLE groups, spinal cord ischemia was induced by infrarenal aortic occlusion for 30 minutes. During the occlusion, the same volume of NS or DADLE at the indicated doses was infused continuously through a catheter to the distally clamped abdominal aorta. Heart rate, blood pressure, and core temperature were monitored continuously to evaluate the potential adverse effects of DADLE. Neurologic behavioral function was assessed with the Tarlov scale system at 1, 6, 24, 48, and 72 hours after reperfusion. Neuronal injury evaluation in the ventral horn of the gray matter was evaluated by counting the normal motor neurons at 72 hours after reperfusion. RESULTS: The therapeutic benefits increased at the doses of DADLE from 0.0005 to 0.05 mg/kg and decreased at 0.5 mg/kg, whereas the hemodynamic parameter was suppressed temporarily at the dose of 0.5 mg/kg. CONCLUSIONS: These data revealed that regional administration of DADLE through the abdominal aorta provided dose-dependent protection on spinal cord I/R in rabbits.
[Mh] Termos MeSH primário: Aorta Abdominal/cirurgia
Cateterismo Periférico
Leucina Encefalina-2-Alanina/administração & dosagem
Fármacos Neuroprotetores/administração & dosagem
Traumatismo por Reperfusão/prevenção & controle
Isquemia do Cordão Espinal/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Aorta Abdominal/fisiopatologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Feminino
Infusões Intravenosas
Ligadura
Masculino
Neurônios Motores/efeitos dos fármacos
Neurônios Motores/patologia
Coelhos
Fluxo Sanguíneo Regional
Traumatismo por Reperfusão/patologia
Traumatismo por Reperfusão/fisiopatologia
Isquemia do Cordão Espinal/patologia
Isquemia do Cordão Espinal/fisiopatologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neuroprotective Agents); 63631-40-3 (Enkephalin, Leucine-2-Alanine)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160328
[Lr] Data última revisão:
160328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150117
[St] Status:MEDLINE


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[PMID]:26591199
[Au] Autor:Dontsov AV
[Ti] Título:[THE ANTIOXIDANT EFFECT OF DALARGIN IN PATIENTS WITH CORONARY HEART DISEASE AND METABOLIC SYNDROME].
[So] Source:Eksp Klin Farmakol;78(7):3-6, 2015.
[Is] ISSN:0869-2092
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim of this study was to evaluate the effect of dalargin on the state of lipid peroxidation (LPO) and antioxidant system in patients with coronary heart disease (CHD) and on the background of metabolic syndrome (MS) in a group of 123 patients with stable coronary artery disease and MS (mean age 56.7 ± 5.1 years). For this purpose, the blood redox potential (EP), total antioxidant activity (TAA), level of oxidized low density lipoproteins (LDL), and activity of superoxide dismutase (SOD) were compared between the group receiving a standard medical therapy (ST) for coronary heart disease (group 1, n = 63) and that with supplementary dalargin administration (ST + D) in a dose of 1 mg intranasally twice a day for 10 days (group 2, n = 60), using the same dose for 10 days in the next two months (total 3 courses over 3 months). It was found that patients with CHD + MS upon 3-month ST showed no statistically significant changes in parameters characterizing the oxidative potential of blood (EP) and antioxidant protection of blood (oxidized LDL level, SOD activity). The inclusion of dalargin into therapy (ST + D) led to a significant decrease in the oxidative stress parameters (blood EP by 10.5%, oxidized LDL level by 14%, p < 0.001) and increase in the blood antioxidant properties (SOD activity by 36.1%, TAA by 25.3%, p < 0.001).
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/administração & dosagem
Antioxidantes/metabolismo
Doença das Coronárias
Leucina Encefalina-2-Alanina/análogos & derivados
Peroxidação de Lipídeos/efeitos dos fármacos
Síndrome Metabólica
[Mh] Termos MeSH secundário: Doença das Coronárias/sangue
Doença das Coronárias/tratamento farmacológico
Leucina Encefalina-2-Alanina/administração & dosagem
Feminino
Seres Humanos
Lipoproteínas LDL/sangue
Masculino
Síndrome Metabólica/sangue
Síndrome Metabólica/tratamento farmacológico
Meia-Idade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antioxidants); 0 (Lipoproteins, LDL); 0 (oxidized low density lipoprotein); 63631-40-3 (Enkephalin, Leucine-2-Alanine); V13505565P (enkephalin-Leu, Ala(2)-Arg(6)-)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151124
[St] Status:MEDLINE


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[PMID]:26126577
[Au] Autor:Wang Z; Tang B; Tang F; Li Y; Zhang G; Zhong L; Dong C; He S
[Ad] Endereço:Department of Gastrointestinal Surgery, Guilin Medical University, Affiliated Hospital, Guilin, Guangxi 541001, P.R. China.
[Ti] Título:Protection of rat intestinal epithelial cells from ischemia/reperfusion injury by (D-Ala2, D-Leu5)-enkephalin through inhibition of the MKK7-JNK signaling pathway.
[So] Source:Mol Med Rep;12(3):4079-88, 2015 Sep.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Previous studies have demonstrated that (D­Ala2, D­Leu5)­enkephalin (DADLE) protects rats from hepatic ischemia/reperfusion (I/R) injury. In the present study, DADLE was also observed to alleviate IR­induced intestinal epithelial cell injury in rats by inhibiting mitogen­activated protein kinase kinase 7 (MKK7)­c­Jun N­terminal kinase (JNK) pathway signaling. To investigate the protective effect of DADLE on hypoxia/reoxygenation injury in rat intestinal epithelial cells, rat intestinal epithelial cells were treated with different concentrations of DADLE, following which the cell survival rate was determined using a tetrazolium (MTT) colorimetric assay, and apoptosis was determined using flow cytometry. To confirm whether the protective effect of DADLE was due to its effect on MKK7­JNK signaling, the phosphorylation levels of MKK7 and JNK were analyzed using western blot analysis following treatment with different concentrations of DADLE. The results demonstrated that, following treatment with DADLE, the survival rate of the rat intestinal cells subjected to I/R­induced injury increased significantly and the apoptotic rate decreased in a concentration­dependent manner. In addition, the levels of phosphorylated MKK7 and JNK decreased in a concentration­dependent manner following treatment with DADLE. Silencing the gene expression of MKK7 using small interfering RNA prior to DADLE treatment resulted in a reduction in the protective effects of DADLE on the rat intestinal epithelial cells subjected to I/R injury. Collectively, the results of the present study demonstrated that the protective effects of DADLE in I/R injury in rat intestinal cells occurred through inhibition of the MKK7­JNK pathway.
[Mh] Termos MeSH primário: Leucina Encefalina-2-Alanina/farmacologia
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
MAP Quinase Quinase 7/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
[Mh] Termos MeSH secundário: Animais
Proteínas Reguladoras de Apoptose/metabolismo
Linhagem Celular
Proliferação Celular
Sobrevivência Celular/efeitos dos fármacos
Modelos Animais de Doenças
Células Epiteliais/citologia
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Feminino
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Intestinos/citologia
MAP Quinase Quinase 7/antagonistas & inibidores
MAP Quinase Quinase 7/genética
Masculino
Fosforilação/efeitos dos fármacos
Interferência de RNA
RNA Interferente Pequeno/metabolismo
Ratos
Ratos Sprague-Dawley
Traumatismo por Reperfusão/metabolismo
Traumatismo por Reperfusão/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Neuroprotective Agents); 0 (RNA, Small Interfering); 63631-40-3 (Enkephalin, Leucine-2-Alanine); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.12.2 (MAP Kinase Kinase 7)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:151201
[Lr] Data última revisão:
151201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150702
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2015.3991


  9 / 1536 MEDLINE  
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[PMID]:26027228
[Au] Autor:Cherpakov RA; Grebenchikov OA; Plotnikov EJ; Likhvantsev VV
[Ti] Título:[Comparison of pharmacological renal preconditioning with dalargin and lithium ions in the model of gentamycin-induced acute renal failure].
[So] Source:Anesteziol Reanimatol;60(1):58-63, 2015 Jan-Feb.
[Is] ISSN:0201-7563
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:PURPOSE: To examine the efficacy of renal preconditioning effect of dalargin and lithium ions by observing the model of gentamycin-induced acute renalfailure. MATERIALS AND METHODS: The experiments were performed on white rats, male. The influence of dalargin and lithium ions on the development of gentamycin-induced acute renalfailure was studied in vivo. On the first 24 hours after dalargin injections were terminated, the rats were euthanized humanly. After this we took the blood for a biochemistry study and a renal culture for biochemical test and also for the test of gsk-3ß activity. Concentrations of creatinine and urea were studied in serum. The culture samples of renal tubular epithelium before insertion of gentamycin were incubated in dalargin or lithium ions in different concentrations. After that the substratum was immediately changed to gentamycin in different concentrations also and the incubated for 24 hours. After all the standards MTT-test was performed (based on the ability of living cells to reduce the unpainted form by 3-4,5-dimethylthiazol-2-yl-2,5-difenilterarazola to blue crystalline farmazan). RESULTS: Lithium precondition leads to the 250% increase of gsk-3ß concentration (p = 0.035). The same results were observed after injection of dalargin in 50 mcg/kg concentration. Concentration of creatinine was 44% lower in the dalargin group than in the control group (p = 0.022). Concentration of creatinine was 32% lower in the lithium group than in the control group (p = 0.030). Concentration of urea was 27% lower in the lithium group than in the control group (p = 0.049). Morphological inflammatory changes in the control group were more significant also. In vitro studies showed the maximum efficacy in the lithium group. The most effective dalargin concentration was 5 mg/ml. CONCLUSION: Lithium and dalargine preconditioning lowers the signs of gentamycine induced acute renal failure and damage rate of renal parenchyma in vivo and in vitro.
[Mh] Termos MeSH primário: Lesão Renal Aguda/prevenção & controle
Leucina Encefalina-2-Alanina/análogos & derivados
Gentamicinas/farmacologia
Isquemia/prevenção & controle
Precondicionamento Isquêmico/métodos
Rim/irrigação sanguínea
Cloreto de Lítio/uso terapêutico
[Mh] Termos MeSH secundário: Lesão Renal Aguda/induzido quimicamente
Lesão Renal Aguda/enzimologia
Lesão Renal Aguda/patologia
Animais
Células Cultivadas
Modelos Animais de Doenças
Leucina Encefalina-2-Alanina/administração & dosagem
Leucina Encefalina-2-Alanina/uso terapêutico
Quinase 3 da Glicogênio Sintase/antagonistas & inibidores
Quinase 3 da Glicogênio Sintase/metabolismo
Glicogênio Sintase Quinase 3 beta
Isquemia/complicações
Isquemia/enzimologia
Isquemia/patologia
Rim/efeitos dos fármacos
Rim/enzimologia
Rim/patologia
Testes de Função Renal
Cloreto de Lítio/administração & dosagem
Masculino
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/enzimologia
Fosforilação
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gentamicins); 63631-40-3 (Enkephalin, Leucine-2-Alanine); EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta); EC 2.7.11.1 (Gsk3b protein, rat); EC 2.7.11.26 (Glycogen Synthase Kinase 3); G4962QA067 (Lithium Chloride); V13505565P (enkephalin-Leu, Ala(2)-Arg(6)-)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150602
[St] Status:MEDLINE


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[PMID]:25283992
[Au] Autor:Liu H; Chen B; Zhang Y; Qiu Y; Xia Y; Li S; Yao J
[Ad] Endereço:Department of Anesthesiology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
[Ti] Título:Protective effect of delta opioid agonist [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits.
[So] Source:Neurosci Lett;584:1-6, 2015 Jan 01.
[Is] ISSN:1872-7972
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:[D-Ala(2), D-Leu(5)] enkephalin (DADLE) has been reported to exhibit protective effects against hypoxic or ischemic induced brain insult. However its efficacy on the spinal cord ischemia-reperfusion injury remains unclear. Here we investigate whether DADLE could attenuate ischemia and reperfusion induced neural injury in the rabbit spinal cord. New Zealand white rabbits were subjected to spinal cord ischemia by infrarenal aortic occlusion for 30 min. In the period of spinal cord ischemia, DADLE 0.5 mg/kg or NS were infused continuously into the distal clamped abdominal aorta. The heart rate, blood pressure, and core temperature were monitored continuously during the whole experimental procedure. Then the neurological behavioral function was assessed with Tarlov scale system at 1h, 6h, 24h, 48 h after reperfusion, and neuronal injury evaluation in the ventral horn of gray matter was measured by counting the normal motor neurons at 48 h after reperfusion. Comparing with the control group, the Tarlov scores were significantly higher and the incidences of paraplegia were significantly lower in the DADLE group at four time-point recorded. In addition, the normal neurons numbers in the DADLE group were significant more than those in the control group at 48 h after reperfusion. These results suggested that DADLE infused into the abdominal aorta during ischemia period could attenuate behavioral retardation and the loss of normal motor neuron induced by ischemia-reperfusion in rabbits.
[Mh] Termos MeSH primário: Leucina Encefalina-2-Alanina/uso terapêutico
Fármacos Neuroprotetores/uso terapêutico
Receptores Opioides delta/agonistas
Traumatismo por Reperfusão/prevenção & controle
Isquemia do Cordão Espinal/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Aorta Abdominal
Leucina Encefalina-2-Alanina/administração & dosagem
Feminino
Hemodinâmica/efeitos dos fármacos
Masculino
Neurônios/efeitos dos fármacos
Neurônios/patologia
Fármacos Neuroprotetores/administração & dosagem
Perfusão
Coelhos
Traumatismo por Reperfusão/patologia
Traumatismo por Reperfusão/fisiopatologia
Isquemia do Cordão Espinal/patologia
Isquemia do Cordão Espinal/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Receptors, Opioid, delta); 63631-40-3 (Enkephalin, Leucine-2-Alanine)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:141216
[Lr] Data última revisão:
141216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141007
[St] Status:MEDLINE



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