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[PMID]:27979143
[Au] Autor:Jozanovic M; Hajdukovic M; Galovic O; Kralik G; Kralik Z; Sakac N; Medvidovic-Kosanovic M; Sak-Bosnar M
[Ad] Endereço:Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia. Electronic address: mhorvat2@kemija.unios.hr.
[Ti] Título:Determination of anti-oxidative histidine dipeptides in poultry by microchip capillary electrophoresis with contactless conductivity detection.
[So] Source:Food Chem;221:1658-1665, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A home-made microchip electrophoresis (MCE) device was used to quantitate two biologically important histidine dipeptides, carnosine and anserine, using capacitively coupled contactless conductivity detection (C D), at pH 2.7. The C D detector exhibited a linear response to both carnosine and anserine in the range of 0-200µM for the individual dipeptides and in the range of 0-100µM for each dipeptide when both were present as a mixture. The limit of detections (LOD) for the dipeptides in the mixture were 0.10µM for carnosine and 0.16µM for anserine. Standard addition was used to detemine the accuracy of the method. For carnosine and anserine the recoveries were in the range of 96.7±4.9-106.0±7.5% and 95.3±4.5-105.0±5.1% in thigh muscle and 97.5±5.1-105.0±7.5% and 95.3±5.4-97.3±5.6% in breast muscle, respectively.
[Mh] Termos MeSH primário: Anserina/análise
Carnosina/análise
Eletroforese Capilar/métodos
Músculo Esquelético/química
[Mh] Termos MeSH secundário: Animais
Galinhas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8HO6PVN24W (Carnosine); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27664620
[Au] Autor:Sundekilde UK; Rasmussen MK; Young JF; Bertram HC
[Ad] Endereço:Department of Food Science, Kirstinebjergvej 10, 5792 Årslev and Blichers allé 20, 8830 Tjele, Aarhus University, Denmark.
[Ti] Título:High resolution magic angle spinning NMR spectroscopy reveals that pectoralis muscle dystrophy in chicken is associated with reduced muscle content of anserine and carnosine.
[So] Source:Food Chem;217:151-154, 2017 Feb 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Increased incidences of pectoralis muscle dystrophy are observed in commercial chicken products, but the muscle physiological causes for the condition remain to be identified. In the present study a high-resolution magic angle spinning (HR-MAS) proton ((1)H) NMR spectroscopic examination of intact pectoralis muscle samples (n=77) were conducted to explore metabolite perturbations associated with the muscle dystrophy condition for the very first time. Both in chicken with an age of 21 and 31days, respectively, pectoralis muscle dystrophy was associated with a significantly lower content of anserine (p=0.034), carnosine (p=0.019) and creatine (p=0.049). These findings must be considered intriguing as they corroborate that characteristic muscle di-peptides composed of ß-alanine and histidine derivatives such as anserine are extremely important in homeostasis of contractile muscles as a results of their role as buffering, anti-oxidative, and anti-glycation capacities.
[Mh] Termos MeSH primário: Anserina/análise
Carnosina/análise
Espectroscopia de Ressonância Magnética/métodos
Distrofias Musculares
Músculos Peitorais/química
[Mh] Termos MeSH secundário: Animais
Galinhas/metabolismo
Distrofias Musculares/patologia
Músculos Peitorais/patologia
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8HO6PVN24W (Carnosine); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27584013
[Au] Autor:Vistoli G; Colzani M; Mazzolari A; Maddis DD; Grazioso G; Pedretti A; Carini M; Aldini G
[Ad] Endereço:Department of Pharmaceutical Sciences, Università degli Studi di Milano, via Mangiagalli 25, 20133 Milan, Italy.
[Ti] Título:Computational approaches in the rational design of improved carbonyl quenchers: focus on histidine containing dipeptides.
[So] Source:Future Med Chem;8(14):1721-37, 2016 Sep.
[Is] ISSN:1756-8927
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: The inhibition of protein carbonylation can play therapeutic roles in several oxidative-based diseases and direct carbonyl quenching appears the most effective inhibition strategies. l-carnosine derivatives are effective and selective quenchers toward 4-hydroxy-2-nonenal even though their activity was never investigated in a fully comparable way. RESULTS: The reported results revealed that anserine, homocarnosine and carnosinamide retain a remarkable quenching activity combined with a satisfactory selectivity. In silico analyses confirmed the key role of flexibility, lipophilicity and nucleophilicity parameters in rationalizing the measured reactivity. CONCLUSION: This study confirms that in silico approaches can be successfully used in the rational design of improved carbonyl quenchers. Physicochemical and stereoelectronic descriptors appear really informative especially when explored by their corresponding property spaces.
[Mh] Termos MeSH primário: Anserina/química
Carnosina/análogos & derivados
Simulação por Computador
Dipeptídeos/química
Dipeptídeos/síntese química
Desenho de Drogas
Histidina/química
[Mh] Termos MeSH secundário: Animais
Carnosina/química
Seres Humanos
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dipeptides); 3650-73-5 (homocarnosine); 4QD397987E (Histidine); 8HO6PVN24W (Carnosine); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE
[do] DOI:10.4155/fmc-2016-0088


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[PMID]:27062388
[Au] Autor:Blancquaert L; Baba SP; Kwiatkowski S; Stautemas J; Stegen S; Barbaresi S; Chung W; Boakye AA; Hoetker JD; Bhatnagar A; Delanghe J; Vanheel B; Veiga-da-Cunha M; Derave W; Everaert I
[Ad] Endereço:Department of Movement and Sports Sciences, Ghent University, Ghent, Belgium.
[Ti] Título:Carnosine and anserine homeostasis in skeletal muscle and heart is controlled by ß-alanine transamination.
[So] Source:J Physiol;594(17):4849-63, 2016 Sep 01.
[Is] ISSN:1469-7793
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:KEY POINTS: Using recombinant DNA technology, the present study provides the first strong and direct evidence indicating that ß-alanine is an efficient substrate for the mammalian transaminating enzymes 4-aminobutyrate-2-oxoglutarate transaminase and alanine-glyoxylate transaminase. The concentration of carnosine and anserine in murine skeletal and heart muscle depends on circulating availability of ß-alanine, which is in turn controlled by degradation of ß-alanine in liver and kidney. Chronic oral ß-alanine supplementation is a popular ergogenic strategy in sports because it can increase the intracellular carnosine concentration and subsequently improve the performance of high-intensity exercises. The present study can partly explain why the ß-alanine supplementation protocol is so inefficient, by demonstrating that exogenous ß-alanine can be effectively routed toward oxidation. ABSTRACT: The metabolic fate of orally ingested ß-alanine is largely unknown. Chronic ß-alanine supplementation is becoming increasingly popular for improving high-intensity exercise performance because it is the rate-limiting precursor of the dipeptide carnosine (ß-alanyl-l-histidine) in muscle. However, only a small fraction (3-6%) of the ingested ß-alanine is used for carnosine synthesis. Thus, the present study aimed to investigate the putative contribution of two ß-alanine transamination enzymes, namely 4-aminobutyrate-2-oxoglutarate transaminase (GABA-T) and alanine-glyoxylate transaminase (AGXT2), to the homeostasis of carnosine and its methylated analogue anserine. We found that, when transfected into HEK293T cells, recombinant mouse and human GABA-T and AGXT2 are able to transaminate ß-alanine efficiently. The reaction catalysed by GABA-T is inhibited by vigabatrin, whereas both GABA-T and AGXT2 activity is inhibited by aminooxyacetic acid (AOA). Both GABA-T and AGXT2 are highly expressed in the mouse liver and kidney and the administration of the inhibitors effectively reduced their enzyme activity in liver (GABA-T for vigabatrin; GABA-T and AGXT2 for AOA). In vivo, injection of AOA in C57BL/6 mice placed on ß-alanine (0.1% w/v in drinking water) for 2 weeks lead to a 3-fold increase in circulating ß-alanine levels and to significantly higher levels of carnosine and anserine in skeletal muscle and heart. By contrast, specific inhibition of GABA-T by vigabatrin did not affect carnosine and anserine levels in either tissue. Collectively, these data demonstrate that homeostasis of carnosine and anserine in mammalian skeletal muscle and heart is controlled by circulating ß-alanine levels, which are suppressed by hepatic and renal ß-alanine transamination upon oral ß-alanine intake.
[Mh] Termos MeSH primário: Anserina/metabolismo
Carnosina/metabolismo
Músculo Esquelético/metabolismo
Miocárdio/metabolismo
Transaminases/metabolismo
beta-Alanina/metabolismo
[Mh] Termos MeSH secundário: Ácido Amino-Oxiacético/farmacologia
Animais
Encéfalo/metabolismo
Inibidores Enzimáticos/farmacologia
GABAérgicos/farmacologia
Células HEK293
Homeostase
Seres Humanos
Rim/metabolismo
Fígado/metabolismo
Masculino
Camundongos Endogâmicos C57BL
RNA Mensageiro/metabolismo
Transaminases/antagonistas & inibidores
Transaminases/genética
Vigabatrina/farmacologia
beta-Alanina/sangue
beta-Alanina/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (GABA Agents); 0 (RNA, Messenger); 11P2JDE17B (beta-Alanine); 14I68GI3OQ (Aminooxyacetic Acid); 8HO6PVN24W (Carnosine); EC 2.6.1.- (Transaminases); EC 2.6.1.44 (Alanine-glyoxylate transaminase); GR120KRT6K (Vigabatrin); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE
[do] DOI:10.1113/JP272050


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[PMID]:26926606
[Au] Autor:Pompan DC
[Ad] Endereço:Salinas, CA, USA.
[Ti] Título:Pes Anserine Bursitis: An Underdiagnosed Cause of Knee Pain in Overweight Women.
[So] Source:Am Fam Physician;93(3):170, 2016 Feb 01.
[Is] ISSN:1532-0650
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Anserina/metabolismo
Artralgia/etiologia
Bursite/complicações
Articulação do Joelho/diagnóstico por imagem
Sobrepeso
[Mh] Termos MeSH secundário: Artralgia/diagnóstico
Bursite/diagnóstico
Bursite/metabolismo
Feminino
Seres Humanos
Meia-Idade
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE


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[PMID]:26682691
[Au] Autor:Hisatsune T; Kaneko J; Kurashige H; Cao Y; Satsu H; Totsuka M; Katakura Y; Imabayashi E; Matsuda H
[Ad] Endereço:Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.
[Ti] Título:Effect of Anserine/Carnosine Supplementation on Verbal Episodic Memory in Elderly People.
[So] Source:J Alzheimers Dis;50(1):149-59, 2016.
[Is] ISSN:1875-8908
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Our goal in this study was to determine whether or not anserine/carnosine supplementation (ACS) is capable of preserving cognitive function of elderly people. In a double-blind randomized controlled trial, volunteers were randomly assigned to an ACS or placebo group at a 1:1 ratio. The ACS group took 1.0 g of an anserine/carnosine (3:1) formula daily for 3 months. Participants were evaluated by psychological tests before and after the 3-month supplementation period. Thirty-nine healthy elderly volunteers (60-78 years old) completed the follow-up tests. Among the tests, delayed recall verbal memory assessed by the Wechsler Memory Scale-Logical Memory showed significant preservation in the ACS group, compared to the placebo group (p = 0.0128). Blood analysis revealed a decreased secretion of inflammatory cytokines, including CCL-2 and IL-8, in the ACS group. MRI analysis using arterial spin labeling showed a suppression in the age-related decline in brain blood flow in the posterior cingulate cortex area in the ACS group, compared to the placebo group (p = 0.0248). In another randomized controlled trial, delayed recall verbal memory showed significant preservation in the ACS group, compared to the placebo group (p = 0.0202). These results collectively suggest that ACS may preserve verbal episodic memory and brain perfusion in elderly people, although further study is needed.
[Mh] Termos MeSH primário: Envelhecimento
Anserina/farmacologia
Carnosina/farmacologia
Memória Episódica
Aprendizagem Verbal/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Idoso
Encéfalo/anatomia & histologia
Encéfalo/efeitos dos fármacos
Citocinas/sangue
Citocinas/genética
Suplementos Nutricionais
Método Duplo-Cego
Feminino
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Processamento de Imagem Assistida por Computador
Masculino
Meia-Idade
Testes Neuropsicológicos
Análise de Sequência com Séries de Oligonucleotídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 8HO6PVN24W (Carnosine); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151220
[St] Status:MEDLINE
[do] DOI:10.3233/JAD-150767


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[PMID]:26574038
[Au] Autor:Kopec W; Wiliczkiewicz A; Jamroz D; Biazik E; Pudlo A; Hikawczuk T; Skiba T; Korzeniowska M
[Ad] Endereço:Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
[Ti] Título:Antioxidant status of turkey breast meat and blood after feeding a diet enriched with histidine.
[So] Source:Poult Sci;95(1):53-61, 2016 Jan.
[Is] ISSN:0032-5791
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to investigate the effects of 1) spray dried blood cells rich in histidine and 2) pure histidine added to feed on the antioxidant status and concentration of carnosine related components in the blood and breast meat of female turkeys. The experiment was performed on 168 Big7 turkey females randomly assigned to 3 dietary treatments: control; control with the addition of 0.18% L-histidine (His); and control with the addition of spray dried blood cells (SDBC). Birds were raised for 103 d on a floor with sawdust litter, with drinking water and feed ad libitum. The antioxidant status of blood plasma and breast muscle was analyzed by ferric reducing ability (FRAP) and by 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging ability. The activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) was analyzed in the blood and breast meat, with the content of carnosine and anserine quantified by HPLC. Proximate analysis as well as amino acid profiling were carried out for the feed and breast muscles. Growth performance parameters also were calculated. Histidine supplementation of the turkey diet resulted in increased DPPH radical scavenging capacity in the breast muscles and blood, but did not result in higher histidine dipeptide concentrations. The enzymatic antioxidant system of turkey blood was affected by the diet with SDBC. In the plasma, the SDBC addition increased both SOD and GPx activity, and decreased GPx activity in the erythrocytes. Feeding turkeys with an SDBC containing diet increased BW and the content of isoleucine and valine in breast muscles.
[Mh] Termos MeSH primário: Anserina/metabolismo
Antioxidantes/metabolismo
Carnosina/metabolismo
Dieta/veterinária
Histidina/metabolismo
Carne/análise
Perus/metabolismo
[Mh] Termos MeSH secundário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Animais
Anserina/sangue
Células Sanguíneas/química
Carnosina/sangue
Suplementos Nutricionais/análise
Feminino
Alimentos em Conserva/análise
Histidina/análise
Músculo Esquelético/química
Oxirredução
Distribuição Aleatória
Perus/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 4QD397987E (Histidine); 8HO6PVN24W (Carnosine); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160122
[Lr] Data última revisão:
160122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151118
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pev311


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[PMID]:26206726
[Au] Autor:Peters V; Klessens CQ; Baelde HJ; Singler B; Veraar KA; Zutinic A; Drozak J; Zschocke J; Schmitt CP; de Heer E
[Ad] Endereço:University Children's Hospital, University of Heidelberg, Im Neuenheimer Feld 672, 69120, Heidelberg, Germany.
[Ti] Título:Intrinsic carnosine metabolism in the human kidney.
[So] Source:Amino Acids;47(12):2541-50, 2015 Dec.
[Is] ISSN:1438-2199
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Histidine-containing dipeptides like carnosine and anserine have protective functions in both health and disease. Animal studies suggest that carnosine can be metabolized within the kidney. The goal of this study was to obtain evidence of carnosine metabolism in the human kidney and to provide insight with regards to diabetic nephropathy. Expression, distribution, and localization of carnosinase-1 (CNDP1), carnosine synthase (CARNS), and taurine transporters (TauT) were measured in human kidneys. CNDP1 and CARNS activities were measured in vitro. CNDP1 and CARNS were located primarily in distal and proximal tubules, respectively. Specifically, CNDP1 levels were high in tubular cells and podocytes (20.3 ± 3.4 and 15 ± 3.2 ng/mg, respectively) and considerably lower in endothelial cells (0.5 ± 0.1 ng/mg). CNDP1 expression was correlated with the degradation of carnosine and anserine (r = 0.88 and 0.81, respectively). Anserine and carnosine were also detectable by HPLC in the renal cortex. Finally, TauT mRNA and protein were found in all renal epithelial cells. In diabetic patients, CNDP1 seemed to be reallocated to proximal tubules. We report compelling evidence that the kidney has an intrinsic capacity to metabolize carnosine. Both CNDP1 and CARNS are expressed in glomeruli and tubular cells. Carnosine-synthesizing and carnosine-hydrolyzing enzymes are localized in distinct compartments in the nephron and increased CNDP1 levels suggest a higher CNDP1 activity in diabetic kidneys.
[Mh] Termos MeSH primário: Carnosina/metabolismo
Regulação da Expressão Gênica
Rim/metabolismo
[Mh] Termos MeSH secundário: Anserina/metabolismo
Cromatografia Líquida de Alta Pressão
Neuropatias Diabéticas/metabolismo
Dipeptidases/metabolismo
Células Endoteliais/metabolismo
Células Epiteliais/metabolismo
Perfilação da Expressão Gênica
Seres Humanos
Hidrólise
Imuno-Histoquímica
Túbulos Renais/metabolismo
Glicoproteínas de Membrana/metabolismo
Proteínas de Membrana Transportadoras/metabolismo
Néfrons/metabolismo
Peptídeo Sintases/metabolismo
Podócitos/metabolismo
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Glycoproteins); 0 (Membrane Transport Proteins); 0 (RNA, Messenger); 148686-53-7 (taurine transporter); 8HO6PVN24W (Carnosine); EC 3.4.13.- (CNDP1 protein, human); EC 3.4.13.- (Dipeptidases); EC 6.3.2.- (Peptide Synthases); EC 6.3.2.11 (carnosine synthetase); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150725
[St] Status:MEDLINE
[do] DOI:10.1007/s00726-015-2045-7


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[PMID]:26001783
[Au] Autor:Drozak J; Piecuch M; Poleszak O; Kozlowski P; Chrobok L; Baelde HJ; de Heer E
[Ad] Endereço:From the Department of Metabolic Regulation and jdrozak@biol.uw.edu.pl.
[Ti] Título:UPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase.
[So] Source:J Biol Chem;290(28):17190-205, 2015 Jul 10.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anserine (ß-alanyl-N(Pi)-methyl-L-histidine), a methylated derivative of carnosine (ß-alanyl-L-histidine), is an abundant constituent of vertebrate skeletal muscles. Although it has been suggested to serve as a proton buffer and radical scavenger, its physiological function remains mysterious. The formation of anserine is catalyzed by carnosine N-methyltransferase, recently identified in chicken as histamine N-methyltransferase-like (HNMT-like) protein. Although the HNMT-like gene is absent in mammalian genomes, the activity of carnosine N-methyltransferase was reported in most mammalian species. In the present investigation, we purified carnosine N-methyltransferase from rat muscles about 2600-fold. Three polypeptides of ∼ 45, 50, and 70 kDa coeluting with the enzyme activity were identified in the preparation. Mass spectrometry analysis of these polypeptides resulted in the identification of UPF0586 protein C9orf41 homolog as the only meaningful candidate. Rat UPF0586 and its yeast, chicken, and human orthologs were expressed in COS-7 cells and purified to homogeneity. Although all recombinant proteins catalyzed the formation of anserine, as confirmed by chromatographic and mass spectrometry analysis, rat UPF0586 was more active on carnosine than other orthologs. Confocal microscopy of HeLa cells expressing recombinant UPF5086 proteins revealed their presence in both cytosol and nucleus. Carnosine and Gly-His were the best substrates for all UPF0586 orthologs studied, although the enzymes also methylated other l-histidine-containing di- and tripeptides. Finally, cotransfection of COS-7 cells with rat or human UPF0586 and carnosine synthase transformed the cells into efficient anserine producers. We conclude that UPF0586 is mammalian carnosine N-methyltransferase and hypothesize that it may also serve as a peptide or protein methyltransferase in eukaryotes.
[Mh] Termos MeSH primário: Anserina/biossíntese
Metiltransferases de Proteína/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Células COS
Carnosina/metabolismo
Cercopithecus aethiops
Galinhas
DNA/genética
Células HEK293
Células HeLa
Seres Humanos
Dados de Sequência Molecular
Músculo Esquelético/enzimologia
Filogenia
Metiltransferases de Proteína/química
Metiltransferases de Proteína/genética
Ratos
Ratos Wistar
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
Homologia de Sequência de Aminoácidos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Recombinant Proteins); 0 (Saccharomyces cerevisiae Proteins); 8HO6PVN24W (Carnosine); 9007-49-2 (DNA); EC 2.1.1.- (Protein Methyltransferases); EC 2.1.1.- (UPF0586 protein, rat); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150524
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.640037


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[PMID]:25997178
[Au] Autor:Akkaya Y; Balci K; Goren Y; Akyuz S; Stricker MC; Stover DD; Ritzhaupt G; Collier WB
[Ad] Endereço:Istanbul University, Faculty of Science, Department of Physics, Vezneciler, 34134 Istanbul, Turkey. Electronic address: yaseminb@istanbul.edu.tr.
[Ti] Título:A vibrational spectroscopy study on anserine and its aqueous solutions.
[So] Source:Spectrochim Acta A Mol Biomol Spectrosc;149:812-21, 2015.
[Is] ISSN:1873-3557
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study based on vibrational spectroscopic measurements and Density Functional Theory (DFT), we aimed for a reliable interpretation of the IR and Raman spectra recorded for anserine in the solid phase and water (H2O) and heavy water (D2O) solutions. Initial DFT calculations at the B3LYP/6-31G(d) searched possible conformers of the anserine zwitterion using a systematic conformational search. The corresponding equilibrium geometrical parameters and vibrational spectral data were determined for each of the stable conformers (in water) by the geometry optimization and hessian calculations performed at the same level of theory using the polarized continuum model (PCM). The same calculations were repeated to determine the most energetically preferred dimer structure for the molecule and the associated geometry, force field and vibrational spectral data. The harmonic force constants obtained from these calculations were scaled by the Scaled Quantum Mechanical Force Field (SQM) method and then used in the calculation of the refined wavenumbers, potential energy distributions, IR and Raman intensities. These refined theoretical data, which confirm the zwitterion structure for anserine in the solid phase or aqueous solvents, revealed the remarkable effects of intermolecular hydrogen bonding on the structural properties and observed IR and Raman spectra of this molecule.
[Mh] Termos MeSH primário: Anserina/química
Análise Espectral Raman
Vibração
[Mh] Termos MeSH secundário: Dimerização
Conformação Molecular
Soluções
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Solutions); HDQ4N37UGV (Anserine)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:150810
[Lr] Data última revisão:
150810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150522
[St] Status:MEDLINE



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