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[PMID]:28120195
[Au] Autor:Lee JM; Lee J; Nam GH; Son BS; Jang MU; Lee SW; Hurh BS; Kim TJ
[Ad] Endereço:Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju, 28644, Republic of Korea.
[Ti] Título:Heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from Bacillus amyloliquefaciens.
[So] Source:J Microbiol;55(2):147-152, 2017 Feb.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:γ-Glutamyltranspeptidase (GGT) catalyzes the cleavage of γ-glutamyl compounds and the transfer of γ-glutamyl moiety to water or to amino acid/peptide acceptors. GGT can be utilized for the generation of γ-glutamyl peptides or glutamic acid, which are used as food taste enhancers. In the present study, Bacillus amyloliquefaciens SMB469 with high GGT activity was isolated from Doenjang, a traditional fermented soy food of Korea. The gene encoding GGT from B. amyloliquefaciens SMB469 (BaGGT469) was cloned from the isolate, and heterologously expressed in E. coli and B. subtilis. For comparison, three additional GGT genes were cloned from B. subtilis 168, B. licheniformis DSM 13, and B. amyloliquefaciens FZB42. The BaGGT469 protein was composed of 591 amino acids. The final protein comprises two separate polypeptide chains of 45.7 and 19.7 kDa, generated via autocatalytic cleavage. The specific activity of BaGGT469 was determined to be 17.8 U/mg with γ-L-glutamyl-p-nitroanilide as the substrate and diglycine as the acceptor. GGTs from B. amyloliquefaciens showed 1.4- and 1.7-fold higher transpeptidase activities than those from B. subtilis and B. licheniformis, respectively. Especially, recombinant B. subtilis expressing BaGGT469 demonstrated 11- and 23-fold higher GGT activity than recombinant E. coli and the native B. amyloliquefaciens, respectively, did. These results suggest that BaGGT469 can be utilized for the enzymatic production of various γ-glutamyl compounds.
[Mh] Termos MeSH primário: Bacillus amyloliquefaciens/enzimologia
Bacillus amyloliquefaciens/genética
gama-Glutamiltransferase/genética
gama-Glutamiltransferase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacillus/enzimologia
Bacillus/genética
Bacillus amyloliquefaciens/metabolismo
Clonagem Molecular
Escherichia coli/genética
Fermentação
Glicilglicina
Peso Molecular
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
República da Coreia
Alimentos de Soja/microbiologia
Especificidade por Substrato
gama-Glutamiltransferase/química
gama-Glutamiltransferase/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 10525P22U0 (Glycylglycine); EC 2.3.2.2 (gamma-Glutamyltransferase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-017-6638-6


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[PMID]:27941311
[Au] Autor:Abousaab A; Warsi J; Salker MS; Lang F
[Ad] Endereço:Department of Cardiology, Vascular Medicine and Physiology, University of Tuebingen, Tuebingen, Germany.
[Ti] Título:ß-Klotho as a Negative Regulator of the Peptide Transporters PEPT1 and PEPT2.
[So] Source:Cell Physiol Biochem;40(5):874-882, 2016.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: ß-Klotho, a transmembrane protein expressed in several tissues including the brain and the kidney, is critically important for inhibition of 1,25(OH)2D3 formation by FGF23. The extracellular domain of Klotho protein could be cleaved off, thus being released into blood or cerebrospinal fluid. Soluble klotho is a ß-glucuronidase participating in the regulation of several ion channels and carriers. The present study explored the effect of ß-Klotho protein on the peptide transporters PEPT1 and PEPT2. METHODS: cRNA encoding PEPT1 or PEPT2 was injected into Xenopus laevis oocytes and glycine-glycine (2 mM)-induced inward current (IGly) taken as measure of glycine-glycine transport. Measurements were made without or with prior 24 h treatment with soluble ß-Klotho protein (30 ng/ml) in the absence and presence of ß-glucuronidase inhibitor D-saccharic acid 1,4-lactone monohydrate (DSAL,10 µM). Ussing chamber experiments were employed to determine electrogenic peptide transport across intestinal epithelia of klotho deficient (kl-/-) and corresponding wild type (kl+/+) mice. RESULTS: IGly was observed in PEPT1 and in PEPT2 expressing oocytes but not in water injected oocytes. In both, PEPT1 and PEPT2 expressing oocytes IGly was significantly decreased by treatment with soluble ß-Klotho protein. As shown for PEPT1, ß-klotho protein decreased significantly the maximal transport rate without significantly modifying the affinity of the carrier. The effect of ß-Klotho on PEPT1 was reversed by DSAL. Intestinal IGly was significantly larger in kl-/- than in kl+/+ mice. CONCLUSION: ß-Klotho participates in the regulation of the peptide transporters PEPT1 and PEPT2.
[Mh] Termos MeSH primário: Glucuronidase/metabolismo
Simportadores/metabolismo
[Mh] Termos MeSH secundário: Animais
Transporte Biológico/efeitos dos fármacos
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Glicoproteínas/farmacologia
Glicilglicina/farmacologia
Seres Humanos
Camundongos
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
Transportador 1 de Peptídeos
Proteínas Recombinantes/farmacologia
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Peptide Transporter 1); 0 (Recombinant Proteins); 0 (Symporters); 0 (beta-glucuronidase inhibitor); 0 (hydrogen-coupled oligopeptide transporter PepT2); 10525P22U0 (Glycylglycine); EC 3.2.1.31 (Glucuronidase); EC 3.2.1.31 (klotho protein)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27438891
[Au] Autor:Jiang X; Andersson M; Chau BT; Wong LY; Villafuerte MK; Kaback HR
[Ti] Título:Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.
[So] Source:Biochemistry;55(31):4326-32, 2016 Aug 09.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:On the periplasmic side of LacY, two conserved Gly-Gly pairs in helices II and XI (Gly46 and Gly370, respectively) and helices V and VIII (Gly159 and Gly262, respectively) allow close packing of each helix pair in the outward (periplasmic)-closed conformation. Previous studies demonstrate that replacing one Gly residue in each Gly-Gly pair with Trp leads to opening of the periplasmic cavity with abrogation of transport activity, but an increased rate of galactoside binding. To further investigate the role of the Gly-Gly pairs, 11 double-replacement mutants were constructed for each pair at positions 46 (helix II) and 262 (helix VIII). Replacement with Ala or Ser results in decreased but significant transport activity, while replacements with Thr, Val, Leu, Asn, Gln, Tyr, Trp, Glu, or Lys exhibit very little or no transport. Remarkably, however, the double mutants bind galactoside with affinities 10-20-fold higher than that of the pseudo-WT or WT LacY. Moreover, site-directed alkylation of a periplasmic Cys replacement indicates that the periplasmic cavity becomes readily accessible in the double-replacement mutants. Molecular dynamics simulations with the WT and double-Leu mutant in the inward-open/outward-closed conformation provide support for this interpretation.
[Mh] Termos MeSH primário: Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/genética
Proteínas de Transporte de Monossacarídeos/química
Proteínas de Transporte de Monossacarídeos/genética
Simportadores/química
Simportadores/genética
[Mh] Termos MeSH secundário: Alquilação
Sequência de Aminoácidos
Substituição de Aminoácidos
Transporte Biológico Ativo
Sequência Conservada
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Glicilglicina/química
Glicilglicina/genética
Lactose/metabolismo
Modelos Moleculares
Simulação de Dinâmica Molecular
Proteínas de Transporte de Monossacarídeos/metabolismo
Mutagênese Sítio-Dirigida
Nitrofenilgalactosídeos/metabolismo
Periplasma/metabolismo
Conformação Proteica
Conformação Proteica em alfa-Hélice
Simportadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (LacY protein, E coli); 0 (Monosaccharide Transport Proteins); 0 (Symporters); 10525P22U0 (Glycylglycine); 28347-45-7 (Nitrophenylgalactosides); 3150-24-1 (4-nitrophenylgalactoside); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b00666


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[PMID]:26967897
[Au] Autor:Li C; Li P; Tan YM; Lam SH; Chan EC; Gong Z
[Ad] Endereço:Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
[Ti] Título:Metabolomic Characterizations of Liver Injury Caused by Acute Arsenic Toxicity in Zebrafish.
[So] Source:PLoS One;11(3):e0151225, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arsenic is one of the most common metalloid contaminants in groundwater and it has both acute and chronic toxicity affecting multiple organs. Details of the mechanism of arsenic toxicity are still lacking and profile studies at metabolic level are very limited. Using gas chromatography coupled with mass spectroscopy (GC/MS), we first generated metabolomic profiles from the livers of arsenic-treated zebrafish and identified 34 significantly altered metabolite peaks as potential markers, including four prominent ones: cholic acid, glycylglycine, glycine and hypotaurine. Combined results from GC/MS, histological examination and pathway analyses suggested a series of alterations, including apoptosis, glycogenolysis, changes in amino acid metabolism and fatty acid composition, accumulation of bile acids and fats, and disturbance in glycolysis related energy metabolism. The alterations in glycolysis partially resemble Warburg effect commonly observed in many cancer cells. However, cellular damages were not reflected in two conventional liver function tests performed, Bilirubin assay and alanine aminotransferase (ALT) assay, probably because the short arsenate exposure was insufficient to induce detectable damage. This study demonstrated that metabolic changes could reflect mild liver impairments induced by arsenic exposure, which underscored their potential in reporting early liver injury.
[Mh] Termos MeSH primário: Arsênico/toxicidade
Doença Hepática Induzida por Substâncias e Drogas/fisiopatologia
Fígado/efeitos dos fármacos
Metaboloma/efeitos dos fármacos
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Animais
Ácidos e Sais Biliares/metabolismo
Bilirrubina/análise
Biomarcadores/análise
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Ácido Cólico/análise
Análise por Conglomerados
Metabolismo Energético/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Glicólise/efeitos dos fármacos
Glicilglicina/análise
Fígado/metabolismo
Fígado/patologia
Análise de Componente Principal
Taurina/análogos & derivados
Taurina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bile Acids and Salts); 0 (Biomarkers); 10525P22U0 (Glycylglycine); 1EQV5MLY3D (Taurine); 5L08GE4332 (hypotaurine); EC 2.6.1.2 (Alanine Transaminase); G1JO7801AE (Cholic Acid); N712M78A8G (Arsenic); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151225


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[PMID]:26627952
[Au] Autor:Marsh BM; Voss JM; Garand E
[Ad] Endereço:Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA.
[Ti] Título:A dual cryogenic ion trap spectrometer for the formation and characterization of solvated ionic clusters.
[So] Source:J Chem Phys;143(20):204201, 2015 Nov 28.
[Is] ISSN:1089-7690
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new experimental approach is presented in which two separate cryogenic ion traps are used to reproducibly form weakly bound solvent clusters around electrosprayed ions and messenger-tag them for single-photon infrared photodissociation spectroscopy. This approach thus enables the vibrational characterization of ionic clusters comprised of a solvent network around large and non-volatile ions. We demonstrate the capabilities of the instrument by clustering water, methanol, and acetone around a protonated glycylglycine peptide. For water, cluster sizes with greater than twenty solvent molecules around a single ion are readily formed. We further demonstrate that similar water clusters can be formed around ions having a shielded charge center or those that do not readily form hydrogen bonds. Finally, infrared photodissociation spectra of D2-tagged GlyGlyH(+)⋅(H2O)1-4 are presented. They display well-resolved spectral features and comparisons with calculations reveal detailed information on the solvation structures of this prototypical peptide.
[Mh] Termos MeSH primário: Acetona/química
Glicilglicina/química
Espectrometria de Massas/instrumentação
Metanol/química
Água/química
[Mh] Termos MeSH secundário: Íons/química
Prótons
Teoria Quântica
Solubilidade
Espectrofotometria Infravermelho
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Ions); 0 (Protons); 059QF0KO0R (Water); 10525P22U0 (Glycylglycine); 1364PS73AF (Acetone); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151202
[Lr] Data última revisão:
151202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151203
[St] Status:MEDLINE
[do] DOI:10.1063/1.4936360


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[PMID]:26599585
[Au] Autor:Ly HG; Mihaylov T; Absillis G; Pierloot K; Parac-Vogt TN
[Ad] Endereço:Laboratory of Bioinorganic Chemistry and ‡Laboratory of Computational Coordination Chemistry, Department of Chemistry, Katholieke Universiteit Leuven , Celestijnenlaan 200F, 3001 Leuven, Belgium.
[Ti] Título:Reactivity of Dimeric Tetrazirconium(IV) Wells-Dawson Polyoxometalate toward Dipeptide Hydrolysis Studied by a Combined Experimental and Density Functional Theory Approach.
[So] Source:Inorg Chem;54(23):11477-92, 2015 Dec 07.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Detailed kinetic studies on the hydrolysis of glycylglycine (Gly-Gly) in the presence of the dimeric tetrazirconium(IV)-substituted Wells-Dawson-type polyoxometalate Na14[Zr4(P2W16O59)2(µ3-O)2(OH)2(H2O)4] · 57H2O (1) were performed by a combination of (1)H, (13)C, and (31)P NMR spectroscopies. The catalyst was shown to be stable under a broad range of reaction conditions. The effect of pD on the hydrolysis of Gly-Gly showed a bell-shaped profile with the fastest hydrolysis observed at pD 7.4. The observed rate constant for the hydrolysis of Gly-Gly at pD 7.4 and 60 °C was 4.67 × 10(-7) s(-1), representing a significant acceleration as compared to the uncatalyzed reaction. (13)C NMR data were indicative for coordination of Gly-Gly to 1 via its amide oxygen and amine nitrogen atoms, resulting in a hydrolytically active complex. Importantly, the effective hydrolysis of a series of Gly-X dipeptides with different X side chain amino acids in the presence of 1 was achieved, and the observed rate constant was shown to be dependent on the volume, chemical nature, and charge of the X amino acid side chain. To give a mechanistic explanation of the observed catalytic hydrolysis of Gly-Gly, a detailed quantum-chemical study was performed. The theoretical results confirmed the nature of the experimentally suggested binding mode in the hydrolytically active complex formed between Gly-Gly and 1. To elucidate the role of 1 in the hydrolytic process, both the uncatalyzed and the polyoxometalate-catalyzed reactions were examined. In the rate-determining step of the uncatalyzed Gly-Gly hydrolysis, a carboxylic oxygen atom abstracts a proton from a solvent water molecule and the nascent OH nucleophile attacks the peptide carbon atom. Analogous general-base activity of the free carboxylic group was found to take place also in the case of polyoxometalate-catalyzed hydrolysis as the main catalytic effect originates from the -C═O···Zr(IV) binding.
[Mh] Termos MeSH primário: Glicilglicina/química
Óxidos/química
Compostos de Tungstênio/química
Zircônio/química
[Mh] Termos MeSH secundário: Catálise
Dimerização
Concentração de Íons de Hidrogênio
Hidrólise
Cinética
Modelos Químicos
Teoria Quântica
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oxides); 0 (Tungsten Compounds); 059QF0KO0R (Water); 10525P22U0 (Glycylglycine); C6V6S92N3C (Zirconium)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151207
[Lr] Data última revisão:
151207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.5b02122


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[PMID]:26363582
[Au] Autor:Rossini AJ; Schlagnitweit J; Lesage A; Emsley L
[Ad] Endereço:Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland; Institut des Sciences Analytiques, Centre de RMN à très hauts champs (CNRS/ENS-Lyon, UCB-Lyon1), Université de Lyon, 69100 Villeurbanne, France.
[Ti] Título:High-resolution NMR of hydrogen in organic solids by DNP enhanced natural abundance deuterium spectroscopy.
[So] Source:J Magn Reson;259:192-8, 2015 Oct.
[Is] ISSN:1096-0856
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We demonstrate that high field (9.4 T) dynamic nuclear polarization (DNP) at cryogenic (∼100 K) sample temperatures enables the rapid acquisition of natural abundance (1)H-(2)H cross-polarization magic angle spinning (CPMAS) solid-state NMR spectra of organic solids. Spectra were obtained by impregnating substrates with a solution of the stable DNP polarizing agent TEKPol in tetrachloroethane. Tetrachloroethane is a non-solvent for the solids, and the unmodified substrates are then polarized through spin diffusion. High quality natural abundance (2)H CPMAS spectra of histidine hydrochloride monohydrate, glycylglycine and theophylline were acquired in less than 2h, providing direct access to hydrogen chemical shifts and quadrupolar couplings. The spectral resolution of the (2)H solid-state NMR spectra is comparable to that of (1)H spectra obtained with state of the art homonuclear decoupling techniques.
[Mh] Termos MeSH primário: Deutério/análise
Espectroscopia de Ressonância Magnética/métodos
[Mh] Termos MeSH secundário: Campos Eletromagnéticos
Etano/análogos & derivados
Etano/química
Glicilglicina/química
Histidina/química
Hidrocarbonetos Clorados/química
Hidrogênio/química
Indicadores e Reagentes
Temperatura Ambiente
Teofilina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hydrocarbons, Chlorinated); 0 (Indicators and Reagents); 10525P22U0 (Glycylglycine); 31UK5E8W7C (tetrachloroethane); 4QD397987E (Histidine); 7YNJ3PO35Z (Hydrogen); AR09D82C7G (Deuterium); C137DTR5RG (Theophylline); L99N5N533T (Ethane)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150914
[St] Status:MEDLINE


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[PMID]:26163404
[Au] Autor:Zhao C; Du H; Xu L; Wang J; Tang L; Cao Y; Li C; Wang Q; Liu Y; Shan F; Feng J; Xu F; Gao P
[Ad] Endereço:Department of Neurology, Shengjing Hospital of China Medical University, 110004 Shenyang, Liaoning, China; Department of Neurology, Dalian (Municipal) Friendship Hospital (Dalian Red Cross Hospital),116001 Dalian, China.
[Ti] Título:Metabolomic analysis revealed glycylglycine accumulation in astrocytes after methionine enkephalin administration exhibiting neuron protective effects.
[So] Source:J Pharm Biomed Anal;115:48-54, 2015 Nov 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Owing to its unrevealed etiology, multiple sclerosis lacks specific therapies up to now. Experiential administration of methionine enkephalin (MENK) on mouse model improved disease manifestations to some extent. In order to gain more insight on the significance of MENK application, a capillary electrophoresis-mass spectrometry (CE-MS) technique was employed to profile intracellular metabolite fluctuation in 5 astrocytoma cell lines challenged by MENK. The processed data were first evaluated through a bioinformatic process to ensure their compatibility with the study aims and then subjected to multivariate analysis. The results indicated that MENK administration increased intracellular tyrosine, phenylalanine, methionine and glycylglycine. Exemplified by U87 cells, glycylglycine inhibited cell proliferation as well as MENK but it also decreased cell nitric oxide excretion which could not be evoked by MENK. The neuron protective effects were also mirrored by the increased expression of some genes related to remyelination. This study demonstrated CE-MS to be a promising tool for cell metabolomic analysis and benefited the therapeutic exploring of multiple sclerosis with respect to metabolism intervention.
[Mh] Termos MeSH primário: Astrócitos/efeitos dos fármacos
Encefalina Metionina/farmacologia
Glicilglicina/metabolismo
Metabolômica/métodos
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
[Mh] Termos MeSH secundário: Animais
Astrócitos/imunologia
Astrócitos/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Análise por Conglomerados
Citocinas/genética
Eletroforese Capilar
Glicilglicina/farmacologia
Seres Humanos
Espectrometria de Massas
Metabolômica/instrumentação
Esclerose Múltipla/metabolismo
Análise Multivariada
Óxido Nítrico/metabolismo
Ratos
Receptores Opioides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Neuroprotective Agents); 0 (Receptors, Opioid); 0 (methionine-enkephalin receptor); 10525P22U0 (Glycylglycine); 31C4KY9ESH (Nitric Oxide); 58569-55-4 (Enkephalin, Methionine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150921
[Lr] Data última revisão:
150921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150712
[St] Status:MEDLINE


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[PMID]:26149753
[Au] Autor:Koyambo-Konzapa SJ; Minguirbara A; Nsangou M
[Ad] Endereço:Centre for Atomic, Molecular Physics and Quantum Optics, Faculty of Science, The University of Douala, PO Box 8580, Douala, Cameroon, koyamboj@yahoo.fr.
[Ti] Título:Solvent effects on the structures and vibrational features of zwitterionic dipeptides: L-diglycine and L-dialanine.
[So] Source:J Mol Model;21(8):189, 2015 Aug.
[Is] ISSN:0948-5023
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Calculations were done by applying the B3LYP/6-31++G(d) method on the zwitterionic L-diglycine and L-dialanine to study the solvent effects on their structures and vibrational features. Three models of solvation (implicit, explicit, and explicit in implicit) were used and the subsequent resulting values compared. Even though both dipeptides surrounded by 12 water molecules seem sufficient to stabilize their zwitterionic characters, notably to avoid the proton transfer between the backbone (N t H[Formula: see text], COO (-)) groups, the hybrid model of solvation (explicit in implicit noted 12W/Continuum) appears to be in better agreement with available IR and Raman experiments than explicit and implicit models. The harmonic vibrational modes derived from geometry optimization of L-diglycine and L-dialanine in 12W/Continuum, agree with the available IR and Raman experimental values within 1 % for L-diglycine and 2 % for L-dialanine, and they appear more accurate than those found using the explicit model (12W). Graphical Abstract DFT/6-31++G∗ Optimized structures of L-diglycine (top) and L-dialanine (bottom) surrounded by 12 water molecules all embedded in a continuum.
[Mh] Termos MeSH primário: Dipeptídeos/química
Glicilglicina/química
Modelos Moleculares
Solventes/química
Água/química
[Mh] Termos MeSH secundário: Análise Espectral Raman
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dipeptides); 0 (Solvents); 059QF0KO0R (Water); 10525P22U0 (Glycylglycine); 2867-20-1 (alanylalanine)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150708
[St] Status:MEDLINE
[do] DOI:10.1007/s00894-015-2718-x


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[PMID]:26138652
[Au] Autor:Ma G; Shi B; Liu J; Zhang H; YinTao Z; Lou X; Liang D; Hou Y; Wan S; Yang W
[Ad] Endereço:Department of General Intensive Care Unit, Songjiang Hospital Affiliated to First People's Hospital, Shanghai Jiao Tong University, No. 746, Zhongshan Road, Shanghai, 201600, China.
[Ti] Título:Nod2-Rip2 Signaling Contributes to Intestinal Injury Induced by Muramyl Dipeptide Via Oligopeptide Transporter in Rats.
[So] Source:Dig Dis Sci;60(11):3264-70, 2015 Nov.
[Is] ISSN:1573-2568
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: PepT1 can transport bacterial oligopeptide products and induce intestinal inflammation. Our aim was to investigate the mechanism of the small intestine injury induced by bacterial oligopeptide product muramyl dipeptide (MDP) which is transported by PepT1. METHODS: We perfused the jejunum with a solution with or without MDP, or with a solution of MDP + Gly-Gly and explored the degree of inflammation to determine the role of PepT1-Nod2 signaling pathway in small intestine mucosa. RESULTS: MDP perfusion induced inflammatory cell accumulation and intestinal damage, accompanied by an increase in mucosal Nod2 and Rip2 transcript expression. NFκB activity and inflammatory cytokine expression, including serum levels of TNF-α, IL-1ß, and IL-6, increased in the MDP group compared to the controls; these effects were reversed by perfusion of the nutritional dipeptide Gly-Gly. CONCLUSION: MDP can be transported through PepT1, causing inflammatory damage in the rat small intestine. Nod2-Rip2-NFκB signaling involved in the small intestinal inflammatory injury caused by MDP which is transported through PepT1.
[Mh] Termos MeSH primário: Acetilmuramil-Alanil-Isoglutamina/toxicidade
Enterite/induzido quimicamente
Mucosa Intestinal/efeitos dos fármacos
Jejuno/efeitos dos fármacos
Proteína Adaptadora de Sinalização NOD2/metabolismo
Proteína Serina-Treonina Quinase 2 de Interação com Receptor/metabolismo
Simportadores/metabolismo
[Mh] Termos MeSH secundário: Acetilmuramil-Alanil-Isoglutamina/metabolismo
Animais
Citocinas/metabolismo
Enterite/enzimologia
Enterite/patologia
Glicilglicina/farmacologia
Mediadores da Inflamação/metabolismo
Mucosa Intestinal/enzimologia
Mucosa Intestinal/patologia
Jejuno/enzimologia
Jejuno/patologia
Masculino
NF-kappa B/metabolismo
Transportador 1 de Peptídeos
Ratos Sprague-Dawley
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators); 0 (NF-kappa B); 0 (NOD2 protein, rat); 0 (Nod2 Signaling Adaptor Protein); 0 (Peptide Transporter 1); 0 (Slc15a1 protein, rat); 0 (Symporters); 10525P22U0 (Glycylglycine); 53678-77-6 (Acetylmuramyl-Alanyl-Isoglutamine); EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinase 2)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150704
[St] Status:MEDLINE
[do] DOI:10.1007/s10620-015-3762-1



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