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Pesquisa : D12.644.504 [Categoria DeCS]
Referências encontradas : 152 [refinar]
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[PMID]:28772192
[Au] Autor:Sica VP; Rees ER; Raja HA; Rivera-Chávez J; Burdette JE; Pearce CJ; Oberlies NH
[Ad] Endereço:Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27402, United States.
[Ti] Título:In situ mass spectrometry monitoring of fungal cultures led to the identification of four peptaibols with a rare threonine residue.
[So] Source:Phytochemistry;143:45-53, 2017 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peptaibols are an intriguing class of fungal metabolites due both to their wide range of reported bioactivities and to the structural variability that can be generated by the exchange of variable amino acid building blocks. In an effort to streamline the discovery of structurally diverse peptaibols, a mass spectrometry surface sampling technique was applied to screen the chemistry of fungal cultures in situ. Four previously undescribed peptaibols, all containing a rare threonine residue, were identified from a fungal culture (MSX53554), which was identified as Nectriopsis Maire (Bionectriaceae, Hypocreales, Ascomycota). These compounds not only increased the known threonine-containing peptaibols by nearly 20%, but also, the threonine residue was situated in a unique place compared to the other reported threonine-containing peptaibols. After the initial in situ detection and characterization, a large-scale solid fermentation culture was grown. The four peptaibols were isolated and characterized by mass spectrometry. In addition, one of the peptaibols was fully characterized by NMR and amino acid analysis using Marfey's reagent and exhibited moderate in vitro anticancer activity.
[Mh] Termos MeSH primário: Hypocreales/química
Peptaibols/química
Peptaibols/isolamento & purificação
Treonina/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Aminoácidos/metabolismo
Antibacterianos/química
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Trichoderma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Anti-Bacterial Agents); 0 (Peptaibols); 2ZD004190S (Threonine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


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[PMID]:28636916
[Au] Autor:Putzu M; Kara S; Afonin S; Grage SL; Bordessa A; Chaume G; Brigaud T; Ulrich AS; Kubar T
[Ad] Endereço:Center for Functional Nanostructures and Institute of Physical Chemistry, Karlsruhe Institute of Technology, Karlsruhe, Germany.
[Ti] Título:Structural Behavior of the Peptaibol Harzianin HK VI in a DMPC Bilayer: Insights from MD Simulations.
[So] Source:Biophys J;112(12):2602-2614, 2017 Jun 20.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microsecond molecular dynamics simulations of harzianin HK VI (HZ) interacting with a dimyristoylphosphatidylcholine bilayer were performed at the condition of low peptide-to-lipid ratio. Two orientations of HZ molecule in the bilayer were found and characterized. In the orientation perpendicular to the bilayer surface, HZ induces a local thinning of the bilayer. When inserted into the bilayer parallel to its surface, HZ is located nearly completely within the hydrophobic region of the bilayer. A combination of solid-state NMR and circular dichroism experiments found the latter orientation to be dominant. An extended sampling simulation provided qualitative results and showed the same orientation to be a global minimum of free energy. The secondary structure of HZ was characterized, and it was found to be located in the 3 -helical family. The specific challenges of computer simulation of nonpolar peptides are discussed briefly.
[Mh] Termos MeSH primário: Dimiristoilfosfatidilcolina/química
Proteínas Fúngicas/química
Bicamadas Lipídicas/química
Peptaibols/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Interações Hidrofóbicas e Hidrofílicas
Espectroscopia de Ressonância Magnética
Simulação de Dinâmica Molecular
Peptaibols/genética
Peptaibols/metabolismo
Estrutura Secundária de Proteína
Trichoderma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Lipid Bilayers); 0 (Peptaibols); U86ZGC74V5 (Dimyristoylphosphatidylcholine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE


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[PMID]:28594169
[Au] Autor:Rivera-Chávez J; Raja HA; Graf TN; Burdette JE; Pearce CJ; Oberlies NH
[Ad] Endereço:Department of Chemistry and Biochemistry, University of North Carolina at Greensboro , P.O. Box 26170, Greensboro, North Carolina 27412, United States.
[Ti] Título:Biosynthesis of Fluorinated Peptaibols Using a Site-Directed Building Block Incorporation Approach.
[So] Source:J Nat Prod;80(6):1883-1892, 2017 Jun 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synthetic biological approaches, such as site-directed biosynthesis, have contributed to the expansion of the chemical space of natural products, making possible the biosynthesis of unnatural metabolites that otherwise would be difficult to access. Such methods may allow the incorporation of fluorine, an atom rarely found in nature, into complex secondary metabolites. Organofluorine compounds and secondary metabolites have both played pivotal roles in the development of drugs; however, their discovery and development are often via nonintersecting tracks. In this context, we used the biosynthetic machinery of Trichoderma arundinaceum (strain MSX70741) to incorporate a fluorine atom into peptaibol-type molecules in a site-selective manner. Thus, fermentation of strain MSX70741 in media containing ortho- and meta-F-phenylalanine resulted in the biosynthesis of two new fluorine-containing alamethicin F50 derivatives. The fluorinated products were characterized using spectroscopic (1D and 2D NMR, including F) and spectrometric (HRESIMS/MS ) methods, and their absolute configurations were established by Marfey's analysis. Fluorine-containing alamethicin F50 derivatives exhibited potency analogous to the nonfluorinated parent when evaluated against a panel of human cancer cell lines. Importantly, the biosynthesis of fluorinated alamethicin F50 derivatives by strain MSX70741 was monitored in situ using a droplet-liquid microjunction-surface sampling probe coupled to a hyphenated system.
[Mh] Termos MeSH primário: Produtos Biológicos/química
Hidrocarbonetos Fluorados/metabolismo
Peptaibols/metabolismo
Trichoderma/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antibacterianos/química
Produtos Biológicos/metabolismo
Seres Humanos
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Peptaibols/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biological Products); 0 (Hydrocarbons, Fluorinated); 0 (Peptaibols)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00189


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[PMID]:28261948
[Au] Autor:Marik T; Urbán P; Tyagi C; Szekeres A; Leitgeb B; Vágvölgyi M; Manczinger L; Druzhinina IS; Vágvölgyi C; Kredics L
[Ad] Endereço:Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
[Ti] Título:Diversity Profile and Dynamics of Peptaibols Produced by Green Mould Trichoderma Species in Interactions with Their Hosts Agaricus bisporus and Pleurotus ostreatus.
[So] Source:Chem Biodivers;14(6), 2017 Jun.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Certain Trichoderma species are causing serious losses in mushroom production worldwide. Trichoderma aggressivum and Trichoderma pleuroti are among the major causal agents of the green mould diseases affecting Agaricus bisporus and Pleurotus ostreatus, respectively. The genus Trichoderma is well-known for the production of bioactive secondary metabolites, including peptaibols, which are short, linear peptides containing unusual amino acid residues and being synthesised via non-ribosomal peptide synthetases (NRPSs). The aim of this study was to get more insight into the peptaibol production of T. aggressivum and T. pleuroti. HPLC/MS-based methods revealed the production of peptaibols closely related to hypomurocins B by T. aggressivum, while tripleurins representing a new group of 18-residue peptaibols were identified in T. pleuroti. Putative NRPS genes enabling the biosynthesis of the detected peptaibols could be found in the genomes of both Trichoderma species. In vitro experiments revealed that peptaibols are potential growth inhibitors of mushroom mycelia, and that the host mushrooms may have an influence on the peptaibol profiles of green mould agents.
[Mh] Termos MeSH primário: Agaricales/crescimento & desenvolvimento
Peptaibols/biossíntese
Trichoderma/metabolismo
[Mh] Termos MeSH secundário: Agaricales/efeitos dos fármacos
Agaricus
Genes Fúngicos
Genoma Fúngico
Inibidores do Crescimento
Micoses
Peptaibols/genética
Peptaibols/toxicidade
Pleurotus
Trichoderma/genética
Trichoderma/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Growth Inhibitors); 0 (Peptaibols)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700033


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[PMID]:28000507
[Au] Autor:Tamandegani PR; Zafari D; Marik T; Szekeres A; Vágvölgyi C; Kredics L
[Ad] Endereço:Department of Plant Protection, Bu Ali Sina University , Hamedan , Iran.
[Ti] Título:Peptaibol profiles of Iranian Trichoderma isolates.
[So] Source:Acta Biol Hung;67(4):431-441, 2016 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Five Iranian Trichoderma isolates from species T. viride, T. viridescens, T. asperellum, T. longibrachiatum and T. citrinoviride - selected from the Fungal Collection of the Bu Ali Sina University, Hamedan, Iran - were investigated for their peptaibol production. All examined isolates showed remarkable antibacterial activities during the screening of their extracts for peptaibol content with a Micrococcus luteus test culture. HPLC-ESI-IT MS was used for identification and elucidation of the amino acid sequences of peptaibols. The detected peptaibol compounds contain 20 or 18 amino acid residues and belong to the trichobrachin and trichotoxin groups of peptaibols, respectively. T. longibrachiatum and T. citrinoviride produced trichobrachins, while trichotoxins could be detected in T. viride, T. viridescens and T. asperellum. Out of 37 sequences detetermined, 26 proved to be new, yet undescribed compounds, while others were identified as previously reported trichotoxins (trichotoxin A-50s and T5D2) and trichobrachins (longibrachins AI, AII, AIII, BII and BIII). Compounds within the two groups of detected peptaibols differed from each other only by a single or just a few amino acid changes.
[Mh] Termos MeSH primário: Peptaibols/metabolismo
Trichoderma/metabolismo
[Mh] Termos MeSH secundário: Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
Cromatografia Líquida de Alta Pressão
Irã (Geográfico)
Micrococcus luteus/efeitos dos fármacos
Peptaibols/farmacologia
Peptídeos/metabolismo
Peptídeos/farmacologia
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Peptaibols); 0 (Peptides); 0 (longibrachin LGA I); 0 (longibrachin LGB II); 0 (longibrachin LGB III); 65454-19-5 (trichotoxin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1556/018.67.2016.4.9


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[PMID]:27443977
[Au] Autor:Degenkolb T; Götze L; von Döhren H; Vilcinskas A; Brückner H
[Ad] Endereço:Institute for Insect Biotechnology, Department of Applied Entomology, Interdisciplinary Research Center for BioSystems, Land Use and Nutrition (IFZ), Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
[Ti] Título:Sequences of stilboflavin C: towards the peptaibiome of the filamentous fungus Stilbella (= Trichoderma) flavipes.
[So] Source:J Pept Sci;22(8):517-24, 2016 Aug.
[Is] ISSN:1099-1387
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Filamentous fungi of the genus Stilbella are recognized as an abundant source of naturally occurring α-aminoisobutyric acid-containing peptides. The culture broth of Stilbella (Trichoderma) flavipes CBS 146.81 yielded a mixture of peptides named stilboflavins (SF), and these were isolated and separated by preparative TLC into groups named SF-A, SF-B, and SF-C. Although all three of these groups resolved as single spots on thin-layer chromatograms, HPLC analysis revealed that each of the groups represents very microheterogeneous mixtures of closely related peptides. Here, we report on the sequence analysis of SF-C peptides, formerly isolated by preparative TLC. HPLC coupled to QqTOF-ESI-HRMS provided the sequences of 10 16-residue peptides and five 19-residue peptides, all of which were N-terminally acetylated. In contrast to the previously described SF-A and SF-B peptaibols, SF-C peptaibols contain Ser-Alaol or Ser-Leuol, which are rarely found as C-termini, and repetitive Leu-Aib-Gly sequences, which have not been detected in peptaibols before. Taking the previously determined sequences of SF-A and SF-B into account, the entirety of peptides produced by S. flavipes (the 'peptaibiome') approaches or exceeds 100 non-ribosomally biosynthesized peptaibiotics. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Proteínas Fúngicas/química
Peptaibols/química
Proteoma/química
Trichoderma/química
[Mh] Termos MeSH secundário: Acetilação
Sequência de Aminoácidos
Ácidos Aminoisobutíricos/isolamento & purificação
Cromatografia Líquida de Alta Pressão
Cromatografia em Camada Delgada
Meios de Cultura/química
Proteínas Fúngicas/classificação
Proteínas Fúngicas/isolamento & purificação
Peptaibols/classificação
Peptaibols/isolamento & purificação
Estrutura Secundária de Proteína
Proteoma/classificação
Proteoma/isolamento & purificação
Análise de Sequência de Proteína
Trichoderma/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoisobutyric Acids); 0 (Culture Media); 0 (Fungal Proteins); 0 (Peptaibols); 0 (Proteome); 1E7ZW41IQU (2-aminoisobutyric acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1002/psc.2897


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[PMID]:27039838
[Au] Autor:Dalzini A; Bergamini C; Biondi B; De Zotti M; Panighel G; Fato R; Peggion C; Bortolus M; Maniero AL
[Ad] Endereço:Dipartimento di Chimica, Università di Padova, via Marzolo 1, 35131, Padova, Italy.
[Ti] Título:The rational search for selective anticancer derivatives of the peptide Trichogin GA IV: a multi-technique biophysical approach.
[So] Source:Sci Rep;6:24000, 2016 Apr 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peptaibols are peculiar peptides produced by fungi as weapons against other microorganisms. Previous studies showed that peptaibols are promising peptide-based drugs because they act against cell membranes rather than a specific target, thus lowering the possibility of the onset of multi-drug resistance, and they possess non-coded α-amino acid residues that confer proteolytic resistance. Trichogin GA IV (TG) is a short peptaibol displaying antimicrobial and cytotoxic activity. In the present work, we studied thirteen TG analogues, adopting a multidisciplinary approach. We showed that the cytotoxicity is tuneable by single amino-acids substitutions. Many analogues maintain the same level of non-selective cytotoxicity of TG and three analogues are completely non-toxic. Two promising lead compounds, characterized by the introduction of a positively charged unnatural amino-acid in the hydrophobic face of the helix, selectively kill T67 cancer cells without affecting healthy cells. To explain the determinants of the cytotoxicity, we investigated the structural parameters of the peptides, their cell-binding properties, cell localization, and dynamics in the membrane, as well as the cell membrane composition. We show that, while cytotoxicity is governed by the fine balance between the amphipathicity and hydrophobicity, the selectivity depends also on the expression of negatively charged phospholipids on the cell surface.
[Mh] Termos MeSH primário: Lipopeptídeos/química
Peptaibols/síntese química
Peptaibols/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Proliferação Celular
Sobrevivência Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Células HeLa
Seres Humanos
Peptaibols/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipopeptides); 0 (Peptaibols); 0 (trichogin GA IV)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE
[do] DOI:10.1038/srep24000


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[PMID]:27009018
[Au] Autor:Mohamed-Benkada M; François Pouchus Y; Vérité P; Pagniez F; Caroff N; Ruiz N
[Ad] Endereço:Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d'Oran-Mohamed Boudiaf (USTO-MB), El Mnaouar, B.P. 1505, Bir El Djir, 31000, Oran, Algeria. professionnelle@hotmail.ca.
[Ti] Título:Identification and Biological Activities of Long-Chain Peptaibols Produced by a Marine-Derived Strain of Trichoderma longibrachiatum.
[So] Source:Chem Biodivers;13(5):521-30, 2016 May.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Six long-chain peptaibols, 1 - 6, were identified from agar cultures of a marine-derived Trichoderma longibrachiatum Rifai strain (MMS151) isolated from blue mussels. The structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (ESI-IT-MS) and GC/EI-MS. The long-chain peptaibols exhibited the general building scheme Ac-Aib-Ala-Aib-Ala-Aib-XXX-Gln-Aib-Vxx-Aib-Gly-XXX-Aib-Pro-Vxx-Aib-XXX-Gln-Gln-Pheol and were similar or identical to recurrent 20-residue peptaibols produced by Trichoderma spp. Three new sequences were identified and were called longibrachins A-0, A-II-a, and A-IV-b. The isolated peptaibols were assayed for cytotoxic, antibacterial, and antifungal activities, and acute toxicity on Dipteran larvae.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antifúngicos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Produtos Biológicos/farmacologia
Peptaibols/farmacologia
Trichoderma/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/isolamento & purificação
Antifúngicos/química
Antifúngicos/isolamento & purificação
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Bactérias/efeitos dos fármacos
Produtos Biológicos/química
Produtos Biológicos/isolamento & purificação
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Dípteros/efeitos dos fármacos
Dípteros/embriologia
Ensaios de Seleção de Medicamentos Antitumorais
Fungos/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Peptaibols/química
Peptaibols/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Biological Products); 0 (Peptaibols)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160325
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201500159


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[PMID]:26953507
[Au] Autor:Otto A; Laub A; Wendt L; Porzel A; Schmidt J; Palfner G; Becerra J; Krüger D; Stadler M; Wessjohann L; Westermann B; Arnold N
[Ad] Endereço:Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry , Weinberg 3, D-06120 Halle (Saale), Germany.
[Ti] Título:Chilenopeptins A and B, Peptaibols from the Chilean Sepedonium aff. chalcipori KSH 883.
[So] Source:J Nat Prod;79(4):929-38, 2016 Apr 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Chilean Sepedonium aff. chalcipori strain KSH 883, isolated from the endemic Boletus loyo Philippi, was studied in a polythetic approach based on chemical, molecular, and biological data. A taxonomic study of the strain using molecular data of the ITS, EF1-α, and RPB2 barcoding genes confirmed the position of the isolated strain within the S. chalcipori clade, but also suggested the separation of this clade into three different species. Two new linear 15-residue peptaibols, named chilenopeptins A (1) and B (2), together with the known peptaibols tylopeptins A (3) and B (4) were isolated from the semisolid culture of strain KSH 883. The structures of 1 and 2 were elucidated on the basis of HRESIMS(n) experiments in conjunction with comprehensive 1D and 2D NMR analysis. Thus, the sequence of chilenopeptin A (1) was identified as Ac-Aib(1)-Ser(2)-Trp(3)-Aib(4)-Pro(5)-Leu(6)-Aib(7)-Aib(8)-Gln(9)-Aib(10)-Aib(11)-Gln(12)-Aib(13)-Leu(14)-Pheol(15), while chilenopeptin B (2) differs from 1 by the replacement of Trp(3) by Phe(3). Additionally, the total synthesis of 1 and 2 was accomplished by a solid-phase approach, confirming the absolute configuration of all chiral amino acids as l. Both the chilenopeptins (1 and 2) and tylopeptins (3 and 4) were evaluated for their potential to inhibit the growth of phytopathogenic organisms.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Peptaibols/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antibacterianos/química
Antibacterianos/farmacologia
Basidiomycota/metabolismo
Chile
Hypocreales/química
Estrutura Molecular
Peptaibols/química
Peptaibols/farmacologia
Peptídeos/química
Peptídeos/isolamento & purificação
Peptídeos/farmacologia
Trichoderma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Peptaibols); 0 (Peptides); 0 (chilenopeptin A); 0 (chilenopeptin B); 0 (tylopeptin A); 0 (tylopeptin B)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160422
[Lr] Data última revisão:
160422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160309
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b01018


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[PMID]:26850879
[Au] Autor:Shi WL; Chen XL; Wang LX; Gong ZT; Li S; Li CL; Xie BB; Zhang W; Shi M; Li C; Zhang YZ; Song XY
[Ad] Endereço:State Key Laboratory of Microbial Technology, Marine Biotechnology Research, Shandong University, Jinan 250100, China State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 10010
[Ti] Título:Cellular and molecular insight into the inhibition of primary root growth of Arabidopsis induced by peptaibols, a class of linear peptide antibiotics mainly produced by Trichoderma spp.
[So] Source:J Exp Bot;67(8):2191-205, 2016 Apr.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Trichoderma spp. are well known biocontrol agents that produce a variety of antibiotics. Peptaibols are a class of linear peptide antibiotics mainly produced by Trichoderma Alamethicin, the most studied peptaibol, is reported as toxic to plants at certain concentrations, while the mechanisms involved are unclear. We illustrated the toxic mechanisms of peptaibols by studying the growth-inhibitory effect of Trichokonin VI (TK VI), a peptaibol from Trichoderma longibrachiatum SMF2, on Arabidopsis primary roots. TK VI inhibited root growth by suppressing cell division and cell elongation, and disrupting root stem cell niche maintenance. TK VI increased auxin content and disrupted auxin response gradients in root tips. Further, we screened the Arabidopsis TK VI-resistant mutant tkr1. tkr1 harbors a point mutation in GORK, which encodes gated outwardly rectifying K(+)channel proteins. This mutation alleviated TK VI-induced suppression of K(+)efflux in roots, thereby stabilizing the auxin gradient. The tkr1 mutant also resisted the phytotoxicity of alamethicin. Our results indicate that GORK channels play a key role in peptaibol-plant interaction and that there is an inter-relationship between GORK channels and maintenance of auxin homeostasis. The cellular and molecular insight into the peptaibol-induced inhibition of plant root growth advances our understanding of Trichoderma-plant interactions.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Arabidopsis/crescimento & desenvolvimento
Peptaibols/farmacologia
Peptídeos/farmacologia
Raízes de Plantas/crescimento & desenvolvimento
Trichoderma/química
[Mh] Termos MeSH secundário: Alameticina/análogos & derivados
Alameticina/farmacologia
Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/metabolismo
Proliferação Celular/efeitos dos fármacos
Clonagem Molecular
Ácidos Indolacéticos/metabolismo
Meristema/efeitos dos fármacos
Meristema/crescimento & desenvolvimento
Mutação/genética
Fenótipo
Raízes de Plantas/efeitos dos fármacos
Canais de Potássio/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/crescimento & desenvolvimento
Nicho de Células-Tronco/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Arabidopsis Proteins); 0 (Indoleacetic Acids); 0 (Peptaibols); 0 (Peptides); 0 (Potassium Channels); 27061-78-5 (Alamethicin); 97242-16-5 (trichokonin VI)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160207
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erw023



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