Base de dados : MEDLINE
Pesquisa : D12.776.034.180 [Categoria DeCS]
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[PMID]:29254927
[Au] Autor:Guangqi L; Congjiao S; Guiqin W; Fengying S; Aiqiao L; Hao S; Ning Y
[Ad] Endereço:1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. Beijing Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China.
[Ti] Título:Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness.
[So] Source:Yi Chuan;39(11):1102-1111, 2017 Nov 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Brown eggs are popular in many countries, and consumers regard eggshell brownness as an important indicator of egg quality. Brown eggshell color is controlled by polygene. However, the responsible genes and detailed molecular mechanisms regulating eggshell brownness have not been defined. In the present study, we applied the RNA-seq technology to analyze the transcriptome data of the shell gland epithelium of hens and investigated the candidate genes associated with eggshell brownness. The results indicated that 8461 genes were expressed in the shell gland epithelium, of which 34 genes were differentially expressed in hens laying dark vs. light brown eggs. Functional analysis revealed that two genes, ovotransferrin (TF) and heat-shock protein 70 (HSP70), as well as the oxidative phosphorylation pathway were involved in the synthesis and transport of protoporphyrin â…¨, which might influence the formation of eggshell brownness and result in different shades of brown.
[Mh] Termos MeSH primário: Galinhas/genética
Casca de Ovo
Genes Reguladores/fisiologia
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Cor
Conalbumina/fisiologia
Proteínas de Choque Térmico HSP70/fisiologia
Protoporfirinas/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HSP70 Heat-Shock Proteins); 0 (Protoporphyrins); 1391-06-6 (Conalbumin); C2K325S808 (protoporphyrin IX)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.16288/j.yczz.17-111


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[PMID]:28710283
[Au] Autor:Constantinescu P; Brown RA; Wyatt AR; Ranson M; Wilson MR
[Ti] Título:Amorphous protein aggregates stimulate plasminogen activation, leading to release of cytotoxic fragments that are clients for extracellular chaperones.
[So] Source:J Biol Chem;292(35):14425-14437, 2017 Sep 01.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The misfolding of proteins and their accumulation in extracellular tissue compartments as insoluble amyloid or amorphous protein aggregates are a hallmark feature of many debilitating protein deposition diseases such as Alzheimer's disease, prion diseases, and type II diabetes. The plasminogen activation system is best known as an extracellular fibrinolytic system but was previously reported to also be capable of degrading amyloid fibrils. Here we show that amorphous protein aggregates interact with tissue-type plasminogen activator and plasminogen, via an exposed lysine-dependent mechanism, to efficiently generate plasmin. The insoluble aggregate-bound plasmin is shielded from inhibition by α -antiplasmin and degrades amorphous protein aggregates to release smaller, soluble but relatively hydrophobic fragments of protein (plasmin-generated protein fragments (PGPFs)) that are cytotoxic. , both endothelial and microglial cells bound and internalized PGPFs before trafficking them to lysosomes. Clusterin and α -macroglobulin bound to PGPFs to significantly ameliorate their toxicity. On the basis of these findings, we hypothesize that, as part of the extracellular proteostasis system, the plasminogen activation system may work synergistically with extracellular chaperones to safely clear large and otherwise pathological protein aggregates from the body.
[Mh] Termos MeSH primário: Fibrinolisina/metabolismo
Microglia/efeitos dos fármacos
Fragmentos de Peptídeos/toxicidade
Ativadores de Plasminogênio/toxicidade
Agregados Proteicos
Ativador de Plasminogênio Tecidual/metabolismo
alfa 2-Antiplasmina/metabolismo
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Animais
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Clusterina/química
Clusterina/metabolismo
Conalbumina/química
Conalbumina/metabolismo
Endotélio Vascular/efeitos dos fármacos
Endotélio Vascular/metabolismo
Endotélio Vascular/patologia
Endotélio Vascular/ultraestrutura
Fibrinolisina/antagonistas & inibidores
Fibrinolisina/química
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Camundongos
Microglia/metabolismo
Microglia/patologia
Microglia/ultraestrutura
Mutação
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Plasminogênio/química
Plasminogênio/metabolismo
Ativadores de Plasminogênio/química
Ativadores de Plasminogênio/genética
Ativadores de Plasminogênio/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Solubilidade
Superóxido Dismutase-1/química
Superóxido Dismutase-1/genética
Superóxido Dismutase-1/metabolismo
Ativador de Plasminogênio Tecidual/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CLU protein, human); 0 (Clusterin); 0 (Peptide Fragments); 0 (Protein Aggregates); 0 (Recombinant Proteins); 0 (SERPINF2 protein, human); 0 (SOD1 protein, human); 0 (alpha-2-Antiplasmin); 1391-06-6 (Conalbumin); 9001-91-6 (Plasminogen); EC 1.15.1.1 (Superoxide Dismutase-1); EC 3.4.21.- (Plasminogen Activators); EC 3.4.21.68 (PLAT protein, human); EC 3.4.21.68 (Tissue Plasminogen Activator); EC 3.4.21.7 (Fibrinolysin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.786657


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[PMID]:28648632
[Au] Autor:Chaipayang S; Songsiriritthigul C; Chen CJ; Palacios PM; Pierce BS; Jangpromma N; Klaynongsruang S
[Ad] Endereço:Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
[Ti] Título:Purification, characterization, cloning and structural analysis of Crocodylus siamensis ovotransferrin for insight into functions of iron binding and autocleavage.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;212:59-69, 2017 Oct.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ovotransferrin (OTf), the major protein constituent of egg white, is of great interest due to its pivotal role in biological iron transport and storage processes and its spontaneous autocleavage into peptidic fragments with alternative biological properties, such as antibacterial and antioxidant activities. However, despite being well-investigated in avian, a detailed elucidation of the structure-function relationship of ovotransferrins in the closely related order of Crocodilia has not been reported to date. In this study, electron paramagnetic resonance (EPR) confirmed the presence of two spectroscopically distinct ferric iron binding sites in Crocodylus siamensis OTf (cOTf), but implied a five-fold lower quantity of bound iron than in hen OTf (hOTf). In addition, quantitative estimation of free sulfhydryl groups revealed slight differences to hOTf. To gain a better structural understanding of cOTf, we found a cOTf gene consisting of an open reading frame of 2040bp and encoding a protein of 679 amino acids. In silico prediction of the three-dimensional structure of cOTf and comparison with hOTf revealed four evolutionarily conserved iron-binding sites in both N- and C-lobes, as well as the presence of only 13 of the 15 disulfide bonds in hOTf. This evolutionary loss of disulfide linkages in conjunction with the lack of hydrogen bonding from a dilysine trigger in the C-lobe are presumed to affect the iron binding and autocleavage character of cOTf. As a result, cOTf may be capable of exerting a more diverse array of functions compared to its avian counterparts; for instance, ion buffering, antioxidant and antimicrobial activities.
[Mh] Termos MeSH primário: Jacarés e Crocodilos/genética
Jacarés e Crocodilos/metabolismo
Conalbumina/genética
Conalbumina/metabolismo
Ferro/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação
Conalbumina/química
Dissulfetos/química
Espectroscopia de Ressonância de Spin Eletrônica
Feminino
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/metabolismo
Modelos Moleculares
Filogenia
Ligação Proteica
Dobramento de Proteína
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Free Radical Scavengers); 0 (Recombinant Proteins); 1391-06-6 (Conalbumin); E1UOL152H7 (Iron)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


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[PMID]:28386310
[Au] Autor:Chen S; Jiang H; Peng H; Wu X; Fang J
[Ad] Endereço:College of Bioscience and Biotechnology and College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
[Ti] Título:The Utility of Ovotransferrin and Ovotransferrin-Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases.
[So] Source:Oxid Med Cell Longev;2017:6504518, 2017.
[Is] ISSN:1942-0994
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several of the most prevalent etiological factors which contribute towards global death rates are associated with cardiovascular diseases (CVDs), which include a range of conditions such as angina, rheumatic heart disease, and venous thrombosis. Extensive research has been conducted into the role played by oxidative stress and inflammation in the functional transformations associated with the progression of CVDs, while the research findings from these investigations have been both fruitful and informative. In view of the adverse secondary effects that result from the clinical administration of many synthetic medications, research which explored the treatment of severe and long-lasting conditions, including CVDs, has primarily centered on the potential benefits displayed by natural agents, one of which is food protein-based bioactive peptides. Most importantly, previous research has revealed the possible benefits associated with these products' anti-inflammatory and antioxidant characteristics. In light of these considerations, this paper aims to review the degree to which ovotransferrin (otrf, also referred to as conalbumin) and otrf-derived peptides, including IRW, IQW, and KVREGT, are, by virtue of their anti-inflammatory and antioxidant characteristics, viable treatment agents for endothelial dysfunction and the prevention of CVD.
[Mh] Termos MeSH primário: Doenças Cardiovasculares/prevenção & controle
Doenças Cardiovasculares/terapia
Conalbumina/fisiologia
Ovalbumina/farmacologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Conalbumina/metabolismo
Células Endoteliais/efeitos dos fármacos
Seres Humanos
Ovalbumina/genética
Peptídeos/genética
Peptídeos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Peptides); 1391-06-6 (Conalbumin); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1155/2017/6504518


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[PMID]:28275769
[Au] Autor:Liu L; Xu M; Tu Y; Du H; Zhou Y; Zhu G
[Ad] Endereço:Key Laboratory of Natural Product and Functional Food of Jiangxi, Nanchang 330045, China.
[Ti] Título:Immunomodulatory effect of protease hydrolysates from ovotransferrin.
[So] Source:Food Funct;8(4):1452-1459, 2017 Apr 19.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proteins and bioactive peptides in avian egg white exert diverse biological activities. This study is designed to investigate the effect of protease hydrolysates from ovotransferrin (OVT) on bone marrow-derived dendritic cells (BMDCs) maturation. The results show that OVT-derived pepsin hydrolysate effectively inhibits lipopolysaccharide (LPS)-induced BMDCs maturation by reducing the expression levels of MHC-II, CD83, CD86 and the production of TNF-α, IL-12p70, and RANTES, but increases the production of IL-10. In addition, OVT-derived pepsin hydrolysate impairs the ability of LPS-stimulated BMDCs to induce allogeneic T lymphocyte proliferation and decreases the production of IFN-γ by activated T cells. In contrast, OVT-derived trypsin hydrolysate induces DCs maturation in terms of increasing the expression levels of MHC-II and the costimulatory molecules CD83 and CD86 and the production of TNF-α, IL-12p70 and RANTES. Furthermore, OVT-derived trypsin hydrolysate improves the ability of LPS-stimulated DCs to induce allogeneic T lymphocyte activation. Blockage of LPS-induced p38 MAPK and JNK activation and inducing ERK activation contribute to the inhibitory effect of OVT-derived pepsin hydrolysate on DCs, whereas OVT-derived trypsin hydrolysate induces DCs maturation through JNK and ERK activation. These results indicate that OVT-derived protease hydrolysate have an immunomodulatory function and could be applied as a potential functional food ingredient to regulate body immunity by modulating DC maturation.
[Mh] Termos MeSH primário: Conalbumina/química
Conalbumina/farmacologia
Células Dendríticas/efeitos dos fármacos
Fatores Imunológicos/química
Fatores Imunológicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD/genética
Antígenos CD/imunologia
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Galinhas
Células Dendríticas/citologia
Células Dendríticas/imunologia
Feminino
Hidrólise
Imunoglobulinas/genética
Imunoglobulinas/imunologia
Interleucina-10/genética
Interleucina-10/imunologia
Interleucina-12/genética
Interleucina-12/imunologia
Glicoproteínas de Membrana/genética
Glicoproteínas de Membrana/imunologia
Camundongos
Camundongos Endogâmicos ICR
Hidrolisados de Proteína/química
Hidrolisados de Proteína/farmacologia
Tripsina/química
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD83 antigen); 0 (Immunoglobulins); 0 (Immunologic Factors); 0 (Membrane Glycoproteins); 0 (Protein Hydrolysates); 0 (Tumor Necrosis Factor-alpha); 130068-27-8 (Interleukin-10); 1391-06-6 (Conalbumin); 187348-17-0 (Interleukin-12); EC 3.4.21.4 (Trypsin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1039/c6fo01669c


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[PMID]:28159460
[Au] Autor:Prajanban BO; Jangpromma N; Araki T; Klaynongsruang S
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand; Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
[Ti] Título:Antimicrobial effects of novel peptides cOT2 and sOT2 derived from Crocodylus siamensis and Pelodiscus sinensis ovotransferrins.
[So] Source:Biochim Biophys Acta;1859(5):860-869, 2017 05.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In light of the increasing threat of bacterial drug resistance to human health on a global scale, research and development of antimicrobial peptides as a novel class of potent antibiotics has gained considerable attention. The present study focuses on the structural evaluation and membrane interaction of two new cationic antimicrobial peptides, cOT2 and sOT2, derived from Siamese crocodile (Crocodylus siamensis) and Chinese softshell turtle (Pelodiscus sinensis) ovotransferrins. Here, cOT1 (+3) and sOT1 (+3) were derived from reptile ovotransferrins by chromatographic purification and characterized by mass spectrometry and N-terminal sequencing analysis. In order to increase the antimicrobial efficacy, two novel peptides, cOT2 (+6) and sOT2 (+5), were designed and synthesized as "naturally-engineered" by primary amino acid sequence extension of cOT1 and sOT1, respectively. These rational designs of modified peptides were assayed in term of antimicrobial activity. These peptides display strong antimicrobial activity against several bacterial strains, e.g. Vibrio cholerae, Bacillus megaterium, and Bacillus pumilus TISTR 905, with MICs of 7-16.1µM. In terms of structural conformation in mimic environments, CD spectroscopic analysis of the secondary peptides structure features revealed fairly the similarity on α-helical content with magainin II. Hence, the modes of actions have been speculated as toroidal and carpet model. Furthermore, the disruption of intact bacterial cells induced by cOT2 and sOT2 was investigated by SEM and AFM. The results provided evidence that cOT2 and sOT2 have the potential to cause different morphological changes of bacterial cells and that these effects can be enhanced by increasing the peptide concentration.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Peptídeos Catiônicos Antimicrobianos/farmacologia
Conalbumina/análise
[Mh] Termos MeSH secundário: Jacarés e Crocodilos
Sequência de Aminoácidos
Animais
Conalbumina/química
Microscopia de Força Atômica
Estrutura Secundária de Proteína
Tartarugas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antimicrobial Cationic Peptides); 1391-06-6 (Conalbumin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


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[PMID]:27744055
[Au] Autor:Khan MV; Ishtikhar M; Rabbani G; Zaman M; Abdelhameed AS; Khan RH
[Ad] Endereço:Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.
[Ti] Título:Polyols (Glycerol and Ethylene glycol) mediated amorphous aggregate inhibition and secondary structure restoration of metalloproteinase-conalbumin (ovotransferrin).
[So] Source:Int J Biol Macromol;94(Pt A):290-300, 2017 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Under physical or chemical stress, proteins tend to form aggregates either highly ordered (amyloid) or unordered (amorphous) causing many pathological disorders in human and loss of proteins functionality in both laboratory conditions and industries during production and storage at commercial level. We investigated the effect of increasing temperature on Conalbumin (CA) and induced aggregation at 65°C. The enhanced Thioflavin T (ThT) and ANS (1-anilinonaphtalene 8-sulfonic acid) fluorescence intensity, show no shift on Congo red binding, additionally, transmission and scanning electron microscopy (TEM) (SEM) reveal amorphous morphology of the aggregate. Our investigation clearly demonstrated that polyols namely Glycerol (GL) and Ethylene glycol (EG) are so staunch to inhibit amorphous aggregates via restoring secondary conformation. Addition of polyols (15% GL and 35% EG) significantly decrease the turbidity, Rayleigh scattering ThT and ANS fluorescence intensity. The dynamic light scattering (DLS) data show that hydrodynamic radii (R ) of the aggregates is ∼20 times higher than native CA while nearly similar for GL and EG protected CA due to condensation of core size with little difference.
[Mh] Termos MeSH primário: Conalbumina/química
Etilenoglicol/química
Glicerol/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
Difusão Dinâmica da Luz
Metaloproteases/química
Agregados Proteicos
Estrutura Secundária de Proteína
Tiazóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Aggregates); 0 (Thiazoles); 1391-06-6 (Conalbumin); 2390-54-7 (thioflavin T); EC 3.4.- (Metalloproteases); FC72KVT52F (Ethylene Glycol); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170612
[Lr] Data última revisão:
170612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161017
[St] Status:MEDLINE


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[PMID]:27649793
[Au] Autor:Liao W; Chakrabarti S; Davidge ST; Wu J
[Ad] Endereço:Department of Agricultural, Food & Nutritional Science, ‡Department of Obstetrics & Gynecology, §Department of Physiology, ∥Cardiovascular Research Centre, and ⊥Women and Children's Health Research Institute, University of Alberta , Edmonton, Alberta T6G 2P5, Canada.
[Ti] Título:Modulatory Effects of Egg White Ovotransferrin-Derived Tripeptide IRW (Ile-Arg-Trp) on Vascular Smooth Muscle Cells against Angiotensin II Stimulation.
[So] Source:J Agric Food Chem;64(39):7342-7347, 2016 Oct 05.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The renin angiotensin system (RAS) is a key mediator of blood pressure regulation. Angiotensin II (Ang II), the active component of RAS, is a potent vasoconstrictor that also causes abnormal proliferation, oxidative stress, and inflammation in vascular smooth muscle cells (VSMCs) that contribute to atherosclerotic changes. Egg white ovotransferrin-derived tripeptide IRW (Ile-Arg-Trp) was previously shown to exert antihypertensive effect by reducing Ang II synthesis as well as endothelial cell inflammation and endothelial dysfunction. However, the effects of IRW on VSMCs are still unclear. In the present study, we evaluated the antiproliferative, antioxidant, and anti-inflammatory effects of IRW on VSMCs in the presence of Ang II stimulation. It was found that IRW treatment could attenuate Ang II-stimulated proliferation, superoxide production, and inflammation in VSMCs. These beneficial effects appeared to involve modulation of the NF-κB pathway. These findings could further our understanding on the antihypertensive mechanism of IRW beyond vascular endothelium.
[Mh] Termos MeSH primário: Angiotensina II/farmacologia
Conalbumina/química
Proteínas do Ovo/química
Clara de Ovo/química
Miócitos de Músculo Liso/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Hipertensivos/química
Técnicas de Cultura de Células
Linhagem Celular
Proliferação Celular
Inflamação
Músculo Liso Vascular/citologia
NF-kappa B/metabolismo
Estresse Oxidativo
Ratos
Sistema Renina-Angiotensina
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antihypertensive Agents); 0 (Egg Proteins); 0 (NF-kappa B); 11062-77-4 (Superoxides); 11128-99-7 (Angiotensin II); 1391-06-6 (Conalbumin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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[PMID]:27436445
[Au] Autor:Shimakura H; Uchiyama J; Saito T; Miyaji K; Fujimura M; Masuda K; Okamoto N; DeBoer DJ; Sakaguchi M
[Ad] Endereço:Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
[Ti] Título:IgE reactivity to hen egg white allergens in dogs with cutaneous adverse food reactions.
[So] Source:Vet Immunol Immunopathol;177:52-7, 2016 Sep.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dogs with cutaneous adverse food reactions (CAFR) often have specific IgE to food allergens. Egg white, which is majorly composed of ovomucoid, ovalbumin, ovotransferrin, and lysozyme, is a food allergen in dogs. Information of the IgE reactivity to purified egg white allergens supports accurate diagnosis and efficiency treatment in humans. However, to the best of our knowledge, there have been no studies on the IgE reactivity to purified egg white allergens in dogs. Here, we investigated the IgE reactivity to crude and purified allergens of hen egg white in dogs with CAFR. First, when we examined serum samples from 82 dogs with CAFR for specific IgE to crude egg white by ELISA, 9.8% (8/82) of the dogs with CAFR showed the IgE reactivity to crude egg white. We then used sera from the eight dogs with positive IgE reactivity to crude egg white to examine the IgE reactivity to four purified allergens, ovomucoid, ovalbumin, ovotransferrin, and lysozyme, by ELISA. We found that 75% (6/8) of the dogs showed IgE reactivity to both ovomucoid and ovalbumin, and that 37.5% (3/8) of the dogs showed IgE reactivity to ovotransferrin. None (0/8) showed IgE reactivity to lysozyme. Moreover, validating these results, the immunoblot analyses were performed using the sera of the three dogs showing the highest IgE reactivity to crude egg white. Both anti-ovomucoid and anti-ovalbumin IgE were detected in the sera of these dogs, while anti-ovotransferrin IgE was not detected. Considering these, ovomucoid and ovalbumin appears to be the major egg white allergens in dogs with CAFR.
[Mh] Termos MeSH primário: Alérgenos
Doenças do Cão/imunologia
Hipersensibilidade a Ovo/veterinária
Clara de Ovo/efeitos adversos
Imunoglobulina E/sangue
[Mh] Termos MeSH secundário: Alérgenos/isolamento & purificação
Animais
Especificidade de Anticorpos
Galinhas
Conalbumina/imunologia
Dermatite Atópica/imunologia
Dermatite Atópica/veterinária
Cães
Hipersensibilidade a Ovo/imunologia
Muramidase/imunologia
Ovalbumina/imunologia
Ovomucina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 1391-06-6 (Conalbumin); 37281-36-0 (Ovomucin); 37341-29-0 (Immunoglobulin E); 9006-59-1 (Ovalbumin); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE


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[PMID]:27153130
[Au] Autor:Chatelain M; Gasparini J; Haussy C; Frantz A
[Ti] Título:Trace Metals Affect Early Maternal Transfer of Immune Components in the Feral Pigeon.
[So] Source:Physiol Biochem Zool;89(3):206-12, 2016 May-Jun.
[Is] ISSN:1537-5293
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maternal early transfers of immune components influence eggs' hatching probability and nestlings' survival. They depend on females' own immunity and, because they are costly, on their physiological state. Therefore, trace metals, whether toxic and immunosuppressive (e.g., lead, cadmium, etc.) or necessary and immunostimulant (e.g., zinc, copper, iron, etc.), are likely to affect the amount of immune components transferred into the eggs. It may also vary with plumage eumelanin level, which is known to be linked to immunity, to transfer of antibodies, and to metal detoxification. In feral pigeons (Columba livia) injected with an antigen and experimentally exposed to lead and/or zinc (two highly abundant trace metals in urban areas), we measured specific antibody transfer and concentrations of two antimicrobial proteins (lysozyme and ovotransferrin) in eggs. As expected, lead had negative effects on specific antibody transfer, while zinc positively affected lysozyme egg concentrations. Moreover, eggs from lead-exposed females exhibited higher ovotransferrin concentrations; because it binds metal ions, ovotransferrin may enable egg detoxification and embryo protection. Finally, eggs' lysozyme concentrations increased with plumage darkness of females not exposed to zinc, while the relation was opposite among zinc-exposed females, suggesting that benefits and costs of plumage melanism depend on trace metal environmental levels. Overall, our study underlines the potential ecotoxicological effects of trace metals on maternal transfers of immune components and the role of plumage melanism in modulating these effects.
[Mh] Termos MeSH primário: Anticorpos/metabolismo
Columbidae/metabolismo
Poluentes Ambientais/toxicidade
Imunidade Materno-Adquirida/efeitos dos fármacos
Chumbo/toxicidade
Zinco/toxicidade
[Mh] Termos MeSH secundário: Animais
Columbidae/imunologia
Conalbumina/metabolismo
Feminino
Hemocianinas/imunologia
Muramidase/metabolismo
Óvulo/imunologia
Oligoelementos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Environmental Pollutants); 0 (Trace Elements); 1391-06-6 (Conalbumin); 2P299V784P (Lead); 9013-72-3 (Hemocyanins); EC 3.2.1.17 (Muramidase); FV4Y0JO2CX (keyhole-limpet hemocyanin); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1086/685511



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