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Pesquisa : D12.776.053.100 [Categoria DeCS]
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[PMID]:27293017
[Au] Autor:Du L; Betti M
[Ad] Endereço:Department of Agricultural, Food and Nutritional Sciences, University of Alberta , Edmonton, AB, Canada T6G 2H1.
[Ti] Título:Identification and Evaluation of Cryoprotective Peptides from Chicken Collagen: Ice-Growth Inhibition Activity Compared to That of Type I Antifreeze Proteins in Sucrose Model Systems.
[So] Source:J Agric Food Chem;64(25):5232-40, 2016 Jun 29.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability of chicken collagen peptides to inhibit the growth of ice crystals was evaluated and compared to that of fish antifreeze proteins (AFPs). This ice inhibition activity was assessed using a polarized microscope by measuring ice crystal dimensions in a sucrose model system with and without collagen peptides after seven thermal cycles. The system was stabilized at -25 °C and cycled between -16 and -12 °C. Five candidate peptides with ice inhibition activity were identified using liquid chromatography and tandem mass spectrometry and were then synthesized. Their ice inhibition capacity was compared to that of type I AFPs in a 23% sucrose model system. Specific collagen peptides with certain amino acid sequences reduced the extent of ice growth by approximately 70% at a relatively low concentration (1 mg/mL). These results suggest that specific collagen peptides may act in a noncolligative manner, inhibiting ice crystal growth like type I AFPs, but less efficiently.
[Mh] Termos MeSH primário: Proteínas Anticongelantes/química
Colágeno/química
Crioprotetores/química
Peptídeos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas Anticongelantes Tipo I/química
Galinhas
Cristalização
Congelamento
Gelo/análise
Espectrometria de Massas
Dados de Sequência Molecular
Sacarose/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifreeze Proteins); 0 (Antifreeze Proteins, Type I); 0 (Cryoprotective Agents); 0 (Ice); 0 (Peptides); 57-50-1 (Sucrose); 9007-34-5 (Collagen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b01911


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[PMID]:27283678
[Au] Autor:Chen J; Cai D; Zhang Y
[Ad] Endereço:Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Centre for Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
[Ti] Título:Rapid determination of lipid peroxidation using a novel pyridoxamine-participating ferrous oxidation-sulfosalicylic acid spectrophotometric method.
[So] Source:Food Chem;211:637-44, 2016 Nov 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel method is developed to rapidly analyze lipid peroxidation in edible oils and fatty foods at room temperature, which is called the pyridoxamine-participating ferrous oxidation-sulfosalicylic acid (PFOS) method. The PFOS method evaluates the lipid peroxide value colorimetrically via detecting the pyridoxamine-mediated pigment produced by 5-sulfosalicylic acid and Fe(3+) at 500nm, while the latter is converted from Fe(2+) in the presence of lipid peroxides. The optimized formulation was ethanol (70%, v/v), Fe(2+) (4mmol/L), 5-sulfosalicylic acid (40mmol/L) and pyridoxamine (18mmol/L). The limit of quantitation is 0.087mmol Fe(3+)/L with acceptable reproducibility. In addition, current method has a significant linear correlation with both conventional thiobarbituric acid (R(2)=0.9999) and ferric thiocyanate assays (R(2)=0.9675). This method offers a rapid technique for evaluating lipid peroxidation without heating and sophisticated instrumental procedures. Besides, current method provides a new option to evaluate the lipid peroxidation state and improve the reproducibility of ferrous-oxidation.
[Mh] Termos MeSH primário: Benzenossulfonatos/química
Compostos Ferrosos/química
Peroxidação de Lipídeos/fisiologia
Peróxidos Lipídicos/análise
Piridoxamina/química
Salicilatos/química
[Mh] Termos MeSH secundário: Animais
Proteínas Anticongelantes Tipo I
Oxirredução
Reprodutibilidade dos Testes
Espectrofotometria/métodos
Suínos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Benzenesulfonates); 0 (Ferrous Compounds); 0 (Lipid Peroxides); 0 (Salicylates); 6466NM3W93 (Pyridoxamine); G7036X8B5H (ferrous oxide); L8XED79U3U (sulfosalicylic acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE


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[PMID]:26020125
[Au] Autor:Nishio M; Murakami H; Horiike A; Takahashi T; Hirai F; Suenaga N; Tajima T; Tokushige K; Ishii M; Boral A; Robson M; Seto T
[Ad] Endereço:*The Cancer Institute Hospital of JFCR, Tokyo, Japan; †Shizuoka Cancer Center, Shizuoka, Japan; ‡National Kyushu Cancer Center, Fukuoka, Japan; §Novartis Pharma K.K., Tokyo, Japan; ¶Novartis Pharmaceuticals Corporation, East Hanover, New Jersey; and ‖Novartis Institutes for Biomedical Research, Cambridge, Massachusetts.
[Ti] Título:Phase I Study of Ceritinib (LDK378) in Japanese Patients with Advanced, Anaplastic Lymphoma Kinase-Rearranged Non-Small-Cell Lung Cancer or Other Tumors.
[So] Source:J Thorac Oncol;10(7):1058-66, 2015 Jul.
[Is] ISSN:1556-1380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Anaplastic lymphoma kinase (ALK)-rearranged non-small-cell lung cancer (NSCLC) is sensitive to ALK inhibitors, but resistance develops. This study assessed the maximum-tolerated dose, safety, pharmacokinetics (PK), and antitumor activity of ceritinib, a novel ALK inhibitor (ALKi), in Japanese patients with ALK-rearranged malignancies. METHODS: This phase I, multicenter, open-label study (NCT01634763) enrolled adult patients with ALK-rearranged (by fluorescence in situ hybridization and/or immunohistochemistry) locally advanced/metastatic malignancy that had progressed despite standard therapy. The study comprised two parts: dose escalation and dose expansion. Ceritinib (single-dose) was administered orally in the 3-day PK run-in period, then once daily, in 21-day cycles. Adaptive dose escalations were guided by a Bayesian model. RESULTS: Twenty patients (80% with ALKi treatment history [ALKi-pretreated]; 19 NSCLC; one inflammatory myofibroblastic tumor) received ceritinib 300 to 750 mg (19 during dose escalation, one in dose expansion). Two dose-limiting toxicities occurred: grade 3 lipase increase (600 mg); grade 3 drug-induced liver injury (750 mg). The most common adverse events were gastrointestinal (nausea: 95%; diarrhea, vomiting: 75%). Ceritinib PK profile was dose proportional across 300 to 750 mg dosages; steady state was reached by day 15. Overall response rate was 55% (11 of 20 patients). Among patients with NSCLC, partial response was observed in two of four ALKi-naive patients, five of nine crizotinib-pretreated patients, two of four alectinib-pretreated patients, and one of two crizotinib and alectinib/ASP3026 pretreated patients. The ASP3026-pretreated inflammatory myofibroblastic tumor patient achieved partial response. CONCLUSIONS: Ceritinib maximum-tolerated dose was 750 mg once daily in Japanese patients. Antitumor activity was observed irrespective of prior ALKi treatment history. Dose expansion, examining the activity of ceritinib in alectinib-resistant patients, is ongoing.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico
Neoplasias Pulmonares/tratamento farmacológico
Inibidores de Proteínas Quinases/administração & dosagem
Pirimidinas/administração & dosagem
Receptores Proteína Tirosina Quinases/antagonistas & inibidores
Receptores Proteína Tirosina Quinases/genética
Sulfonas/administração & dosagem
[Mh] Termos MeSH secundário: Idoso
Proteínas Anticongelantes Tipo I
Grupo com Ancestrais do Continente Asiático
Carcinoma Pulmonar de Células não Pequenas/enzimologia
Carcinoma Pulmonar de Células não Pequenas/patologia
Relação Dose-Resposta a Droga
Feminino
Rearranjo Gênico
Seres Humanos
Imuno-Histoquímica
Neoplasias Pulmonares/enzimologia
Neoplasias Pulmonares/genética
Neoplasias Pulmonares/patologia
Masculino
Meia-Idade
Inibidores de Proteínas Quinases/efeitos adversos
Pirimidinas/efeitos adversos
Receptores Proteína Tirosina Quinases/metabolismo
Sulfonas/efeitos adversos
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE; MULTICENTER STUDY; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Antineoplastic Agents); 0 (Protein Kinase Inhibitors); 0 (Pyrimidines); 0 (Sulfones); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.10.1 (anaplastic lymphoma kinase); K418KG2GET (ceritinib)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150529
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1097/JTO.0000000000000566


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[PMID]:25786071
[Au] Autor:Bagherzadeh SA; Alavi S; Ripmeester JA; Englezos P
[Ad] Endereço:Department of Chemical and Biological Engineering, The University of British Columbia, 2360 East Mall, Vancouver BC, Canada V6T1Z3. peter.englezos@ubc.ca.
[Ti] Título:Why ice-binding type I antifreeze protein acts as a gas hydrate crystal inhibitor.
[So] Source:Phys Chem Chem Phys;17(15):9984-90, 2015 Apr 21.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antifreeze proteins (AFPs) prevent ice growth by binding to a specific ice plane. Some AFPs have been found to inhibit the formation of gas hydrates which are a serious safety and operational challenge for the oil and gas industry. Molecular dynamics simulations are used to determine the mechanism of action of the winter flounder AFP (wf-AFP) in inhibiting methane hydrate growth. The wf-AFP adsorbs onto the methane hydrate surface via cooperative binding of a set of hydrophobic methyl pendant groups to the empty half-cages at the hydrate/water interface. Each binding set is composed of the methyl side chain of threonine and two alanine residues, four and seven places further down in the sequence of the protein. Understanding the principle of action of AFPs can lead to the rational design of green hydrate inhibitor molecules with potential superior performance.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/química
Gases/química
Água/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação
Cristalização
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Modelos Moleculares
Simulação de Dinâmica Molecular
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Gases); 059QF0KO0R (Water)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150402
[Lr] Data última revisão:
150402
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150319
[St] Status:MEDLINE
[do] DOI:10.1039/c4cp05003g


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[PMID]:24531972
[Au] Autor:Sun T; Lin FH; Campbell RL; Allingham JS; Davies PL
[Ad] Endereço:Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada.
[Ti] Título:An antifreeze protein folds with an interior network of more than 400 semi-clathrate waters.
[So] Source:Science;343(6172):795-8, 2014 Feb 14.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:When polypeptide chains fold into a protein, hydrophobic groups are compacted in the center with exclusion of water. We report the crystal structure of an alanine-rich antifreeze protein that retains ~400 waters in its core. The putative ice-binding residues of this dimeric, four-helix bundle protein point inwards and coordinate the interior waters into two intersecting polypentagonal networks. The bundle makes minimal protein contacts between helices, but is stabilized by anchoring to the semi-clathrate water monolayers through backbone carbonyl groups in the protein interior. The ordered waters extend outwards to the protein surface and likely are involved in ice binding. This protein fold supports both the anchored-clathrate water mechanism of antifreeze protein adsorption to ice and the water-expulsion mechanism of protein folding.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/química
Proteínas de Peixes/química
Dobramento de Proteína
[Mh] Termos MeSH secundário: Alanina/química
Animais
Cristalografia por Raios X
Linguado
Gelo
Estrutura Secundária de Proteína
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Fish Proteins); 0 (Ice); 059QF0KO0R (Water); OF5P57N2ZX (Alanine)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:140328
[Lr] Data última revisão:
140328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140218
[St] Status:MEDLINE
[do] DOI:10.1126/science.1247407


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[PMID]:24531963
[Au] Autor:Sharp KA
[Ad] Endereço:Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
[Ti] Título:Biochemistry. Protein folding, interrupted.
[So] Source:Science;343(6172):743-4, 2014 Feb 14.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/química
Proteínas de Peixes/química
Dobramento de Proteína
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Fish Proteins)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:140217
[Lr] Data última revisão:
140217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140218
[St] Status:MEDLINE
[do] DOI:10.1126/science.1249405


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[PMID]:24112663
[Au] Autor:Kamisli S; Ciftci O; Cetin A; Kaya K; Kamisli O; Celik H
[Ti] Título:Fish oil protects the peripheral and central nervous systems against cisplatin-induced neurotoxicity.
[So] Source:Nutr Neurosci;17(3):116-26, 2014 Apr.
[Is] ISSN:1476-8305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The protective effects of fish oil (FO) on cisplatin (CP)-induced central and peripheral neurotoxicity were investigated in rats. METHODS: Rats (n = 28) were divided equally into four groups, the first group was kept as a control. In the second and third groups, CP and FO were given at doses of 7 mg/kg and 1 softgel/rat/day, respectively. In the fourth group, CP and FO were given together at the same doses. RESULTS: Although CP caused significant oxidative damage, via induction of lipid peroxidation and reduction in the antioxidant defense system potency, FO treatment largely reversed these effects. CP also resulted in histopathological damage, such as apoptosis, and electromyographical changes in the sciatic nerve. FO treatment partially prevented the histopathological and electromyographical effects of CP. DISCUSSION: CP has severe central and peripheral neurotoxic effects in rats and these effects were largely prevented by FO treatment. Thus, it appears that co-administration of FO with CP may be a useful approach to attenuate the negative effects of CP on the nervous system.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/administração & dosagem
Encefalopatias/prevenção & controle
Encéfalo/efeitos dos fármacos
Cisplatino/toxicidade
Doenças do Sistema Nervoso Periférico/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Apoptose/efeitos dos fármacos
Encéfalo/patologia
Encéfalo/fisiopatologia
Encefalopatias/induzido quimicamente
Encefalopatias/fisiopatologia
Eletromiografia
Peroxidação de Lipídeos/efeitos dos fármacos
Masculino
Doenças do Sistema Nervoso Periférico/induzido quimicamente
Doenças do Sistema Nervoso Periférico/fisiopatologia
Ratos
Ratos Sprague-Dawley
Nervo Isquiático/efeitos dos fármacos
Nervo Isquiático/patologia
Nervo Isquiático/fisiopatologia
Substâncias Reativas com Ácido Tiobarbitúrico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Antioxidants); 0 (Thiobarbituric Acid Reactive Substances); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140326
[Lr] Data última revisão:
140326
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131012
[St] Status:MEDLINE
[do] DOI:10.1179/1476830513Y.0000000074


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[PMID]:23860838
[Au] Autor:Ba Y; Mao Y; Galdino L; Günsen Z
[Ad] Endereço:Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA. yba@calstatela.edu
[Ti] Título:Effects of a type I antifreeze protein (AFP) on the melting of frozen AFP and AFP+solute aqueous solutions studied by NMR microimaging experiment.
[So] Source:J Biol Phys;39(1):131-44, 2013 Jan.
[Is] ISSN:0092-0606
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The effects of a type I AFP on the bulk melting of frozen AFP solutions and frozen AFP+solute solutions were studied through an NMR microimaging experiment. The solutes studied include sodium chloride and glucose and the amino acids alanine, threonine, arginine, and aspartic acid. We found that the AFP is able to induce the bulk melting of the frozen AFP solutions at temperatures lower than 0 °C and can also keep the ice melted at higher temperatures in the AFP+solute solutions than those in the corresponding solute solutions. The latter shows that the ice phases were in super-heated states in the frozen AFP+solute solutions. We have tried to understand the first experimental phenomenon via the recent theoretical prediction that type I AFP can induce the local melting of ice upon adsorption to ice surfaces. The latter experimental phenomenon was explained with the hypothesis that the adsorption of AFP to ice surfaces introduces a less hydrophilic water-AFP-ice interfacial region, which repels the ionic/hydrophilic solutes. Thus, this interfacial region formed an intermediate chemical potential layer between the water phase and the ice phase, which prevented the transfer of water from the ice phase to the water phase. We have also attempted to understand the significance of the observed melting phenomena to the survival of organisms that express AFPs over cold winters.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/química
Cloreto de Sódio/química
Temperatura de Transição
Água/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Espectroscopia de Ressonância Magnética
Dados de Sequência Molecular
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Solutions); 059QF0KO0R (Water); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130718
[St] Status:MEDLINE
[do] DOI:10.1007/s10867-012-9291-7


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[PMID]:23085291
[Au] Autor:Hobbs RS; Fletcher GL
[Ad] Endereço:Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL, Canada A1C 5S7.
[Ti] Título:Epithelial dominant expression of antifreeze proteins in cunner suggests recent entry into a high freeze-risk ecozone.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;164(1):111-8, 2013 Jan.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Most marine teleost fishes residing in a high freeze-risk ecozone, such as the coastal waters of Newfoundland during winter, avoid freezing by secreting high concentrations of antifreeze proteins (AFP) into their blood plasma where they can bind to and prevent the growth of ice that enter the fish. Cunner (Tautogolabrus adspersus), which overwinter in such shallow waters are the only known exception. Although this species does produce type I AFP, the plasma levels are too low to be of value as a freeze protectant. Southern and Northern blot analyses carried out in this study establish that the cunner AFP genes belong to a multigene family that is predominantly expressed in external epithelia (skin and gill filaments). These results support the hypothesis that the survival of cunner in icy waters is attributable in part to epithelial AFP that help block ice propagation into their interior milieu. In contrast to the cunner, heterospecifics occupying the same habitat have greater freeze protection because they produce AFP in the liver for export to the plasma as well as in external epithelia. Since the external epithelia would be the first tissue to come into contact with ice it is possible that one of the earliest steps involved in the evolution of freeze resistant fish could have been the expression of AFP in tissues such as the skin. We suggest that this epithelial-dominant AFP expression represents a primitive stage in AFP evolution and propose that cunner began to inhabit "freeze-risk ecozones" more recently than heterospecifics.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/metabolismo
Epitélio/metabolismo
Proteínas de Peixes/metabolismo
Perciformes/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Anticongelantes Tipo I/genética
Sequência de Bases
Transporte Biológico
Northern Blotting
Southern Blotting
Clonagem Molecular/métodos
Temperatura Baixa
DNA Complementar/genética
DNA Complementar/metabolismo
Ecossistema
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Peixes/genética
Regulação da Expressão Gênica
Brânquias/metabolismo
Brânquias/fisiologia
Gelo
Fígado/metabolismo
Dados de Sequência Molecular
Família Multigênica
Perciformes/genética
Perciformes/fisiologia
RNA/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Estações do Ano
Pele/metabolismo
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (DNA, Complementary); 0 (Fish Proteins); 0 (Ice); 63231-63-0 (RNA)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:121126
[Lr] Data última revisão:
121126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121023
[St] Status:MEDLINE


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[PMID]:22657839
[Au] Autor:Calvaresi M; Höfinger S; Zerbetto F
[Ad] Endereço:Dipartimento di Chimica G. Ciamician, Università di Bologna, Bologna, Italy. matteo.calvaresi@studio.unibo.it
[Ti] Título:Local ice melting by an antifreeze protein.
[So] Source:Biomacromolecules;13(7):2046-52, 2012 Jul 09.
[Is] ISSN:1526-4602
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antifreeze proteins, AFP, impede freezing of bodily fluids and damaging of cellular tissues by low temperatures. Adsorption-inhibition mechanisms have been developed to explain their functioning. Using in silico Molecular Dynamics, we show that type I AFP can also induce melting of the local ice surface. Simulations of antifreeze-positive and antifreeze-negative mutants show a clear correlation between melting induction and antifreeze activity. The presence of local melting adds a function to type I AFPs that is unique to these proteins. It may also explain some apparently conflicting experimental results where binding to ice appears both quasipermanent and reversible.
[Mh] Termos MeSH primário: Proteínas Anticongelantes Tipo I/química
Proteínas de Peixes/química
Congelamento
Simulação de Dinâmica Molecular
Água/química
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Ligações de Hidrogênio
Análise de Componente Principal
Estrutura Secundária de Proteína
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifreeze Proteins, Type I); 0 (Fish Proteins); 059QF0KO0R (Water)
[Em] Mês de entrada:1211
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120605
[St] Status:MEDLINE
[do] DOI:10.1021/bm300366f



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